Nitric Oxide Is a Signal for NNR-Mediated Transcription Activation in Paracoccus denitrificans
Houben, Edith, Reijnders, Willem N. M., Spiro, Stephen, Westerhoff, Hans V., Saunders, Neil
By using the ′lacZ gene, the activities of the nirI, nirS, and norC promoters were assayed in the wild type and in NNR-deficient mutants of Paracoccus denitrificans grown under various growth...
Shearer, Neil, Hinsley, Andrew P., Spiro, Stephen
A pleiotropic mutant of Paracoccus denitrificans, which has a severe defect that affects its anaerobic growth when either nitrate, nitrite, or nitrous oxide is used as the terminal electron acceptor...
Heterologous NNR-Mediated Nitric Oxide Signaling in Escherichia coli
Hutchings, Matthew I., Shearer, Neil, Wastell, Sarah, Spiro, Stephen
The transcription factor NNR from Paracoccus denitrificans was expressed in a strain of Escherichia coli carrying a plasmid-borne fusion of the melR promoter to lacZ, with a consensus FNR-binding...
Butland, Gareth, Spiro, Stephen, Watmough, Nicholas J., Richardson, David J.
The bacterial nitric oxide reductase (NOR) is a divergent member of the family of respiratory heme-copper oxidases. It differs from other family members in that it contains an FeB–heme-Fe dinuclear...
Hutchings, Matthew I., Mandhana, Neeraj, Spiro, Stephen
The Escherichia coli norVW genes encode a flavorubredoxin and NADH:(flavo)rubredoxin reductase, respectively, which are involved in nitric oxide detoxification under anaerobic growth conditions. Here...
Hutchings, Matthew I., Crack, Jason C., Shearer, Neil, Thompson, Benjamin J., Thomson, Andrew J., Spiro, Stephen
The Paracoccus denitrificans transcription factor FnrP has been characterized using artificial FNR-dependent promoter-lacZ fusion plasmids in Escherichia coli. FnrP can activate both class I and...
Tucker, Nicholas P., D'Autréaux, Benoît, Studholme, David J., Spiro, Stephen, Dixon, Ray
The Escherichia coli nitric oxide sensor NorR was shown to bind to the promoter region of the norVW transcription unit, forming at least two distinct complexes detectable by gel retardation. Three...
The yjeB (nsrR) Gene of Escherichia coli Encodes a Nitric Oxide-Sensitive Transcriptional Regulator
Bodenmiller, Diane M., Spiro, Stephen
Microarray studies of the Escherichia coli response to nitric oxide and nitrosative stress have suggested that additional transcriptional regulators of this response remain to be characterized. We...
Ryan, Robert P., Fouhy, Yvonne, Lucey, Jean F., Crossman, Lisa C., Spiro, Stephen, He, Ya-Wen, ...
HD-GYP is a protein domain of unknown biochemical function implicated in bacterial signaling and regulation. In the plant pathogen Xanthomonas campestris pv. campestris, the synthesis of virulence...
Nitric Oxide Is a Signal for NNR-Mediated Transcription Activation in Paracoccus denitrificans
Houben, Edith, Reijnders, Willem N. M., Spiro, Stephen, Westerhoff, Hans V., Saunders, Neil
By using the ′lacZ gene, the activities of the nirI, nirS, and norC promoters were assayed in the wild type and in NNR-deficient mutants of Paracoccus denitrificans grown under various growth...
Shearer, Neil, Hinsley, Andrew P., Spiro, Stephen
A pleiotropic mutant of Paracoccus denitrificans, which has a severe defect that affects its anaerobic growth when either nitrate, nitrite, or nitrous oxide is used as the terminal electron acceptor...
Heterologous NNR-Mediated Nitric Oxide Signaling in Escherichia coli
Hutchings, Matthew I., Shearer, Neil, Wastell, Sarah, Spiro, Stephen
The transcription factor NNR from Paracoccus denitrificans was expressed in a strain of Escherichia coli carrying a plasmid-borne fusion of the melR promoter to lacZ, with a consensus FNR-binding...
Butland, Gareth, Spiro, Stephen, Watmough, Nicholas J., Richardson, David J.
The bacterial nitric oxide reductase (NOR) is a divergent member of the family of respiratory heme-copper oxidases. It differs from other family members in that it contains an FeB–heme-Fe dinuclear...
Hutchings, Matthew I., Mandhana, Neeraj, Spiro, Stephen
The Escherichia coli norVW genes encode a flavorubredoxin and NADH:(flavo)rubredoxin reductase, respectively, which are involved in nitric oxide detoxification under anaerobic growth conditions. Here...
Hutchings, Matthew I., Crack, Jason C., Shearer, Neil, Thompson, Benjamin J., Thomson, Andrew J., Spiro, Stephen
The Paracoccus denitrificans transcription factor FnrP has been characterized using artificial FNR-dependent promoter-lacZ fusion plasmids in Escherichia coli. FnrP can activate both class I and...
Tucker, Nicholas P., D'Autréaux, Benoît, Studholme, David J., Spiro, Stephen, Dixon, Ray
The Escherichia coli nitric oxide sensor NorR was shown to bind to the promoter region of the norVW transcription unit, forming at least two distinct complexes detectable by gel retardation. Three...
The yjeB (nsrR) Gene of Escherichia coli Encodes a Nitric Oxide-Sensitive Transcriptional Regulator
Bodenmiller, Diane M., Spiro, Stephen
Microarray studies of the Escherichia coli response to nitric oxide and nitrosative stress have suggested that additional transcriptional regulators of this response remain to be characterized. We...
Ryan, Robert P., Fouhy, Yvonne, Lucey, Jean F., Crossman, Lisa C., Spiro, Stephen, He, Ya-Wen, ...
HD-GYP is a protein domain of unknown biochemical function implicated in bacterial signaling and regulation. In the plant pathogen Xanthomonas campestris pv. campestris, the synthesis of virulence...
Filenko, Nina, Spiro, Stephen, Browning, Douglas F., Squire, Derrick, Overton, Tim W., Cole, Jeff, ...
Successful pathogens must be able to protect themselves against reactive nitrogen species generated either as part of host defense mechanisms or as products of their own metabolism. The regulatory...
Kilic, Mehmet A., Spiro, Stephen, Moore, Geoffrey R.
The stability of Rhodobacter capsulatus bacterioferritin, a 24-meric homopolymer, toward denaturation on variation in pH and temperature, and increasing concentrations of urea and guanidine.HCl was...