Hasmik Keshishian, Terri Addona, Michael Burgess, Eric Kuhn, Steven A. Carr
Biomarker discovery produces lists of candidate markers whose presence and level must be subsequently verified in serum or plasma. Verification represents a paradigm shift from unbiased discovery...
Jacob D. Jaffe, D. R. Mani, Kyriacos C. Leptos, George M. Church, Michael A. Gillette, Steven A. Carr
Quantitative proteomics holds considerable promise for elucidation of basic biology and for clinical biomarker discovery. However, it has been difficult to fulfill this promise due to over-reliance...
Michael J. Huddleston, S. Annan, Steven A. Carr, Smithkline Beecham Pharmaceuticals
Phosphorylation is one of the most important protein posttranslational modifications, playing a critical role in many aspects of cellular signaling. Defining the sites of phosphorylation in a protein...
Xiaolong Zhang, Michael J. Huddleston, Yong Chi, Ray Deshaies, Steven A. Carr, Smithkline Beecham Pharmaceuticals
Yeast Gcn4 protein is a transcription factor that is responsible for the activation of more than 30 genes required for amino acid and purine biosynthesis in response to amino acid or purine...
PEPPeR, a Platform for Experimental Proteomic Pattern Recognition (2006)
Jacob D. Jaffe, D. R. Mani, Kyriacos C. Leptos, George M. Church, Michael A. Gillette, Steven A. Carr
Quantitative proteomics holds considerable promise for elucidation of basic biology and for clinical biomarker discovery. However, it has been difficult to fulfill this promise due to over-reliance...
Chen, Susan Loughery, Huddleston, Michael J., Shou, Wenying, Deshaies, Raymond J., Annan, Roland S., Carr, Steven A.
Prior to anaphase in Saccharomyces cerevisiae, Cdc14 protein phosphatase is sequestered within the nucleolus and inhibited by Net1, a component of the RENT complex in budding yeast. During anaphase...
Negative regulation of Gcn4 and Msn2 transcription factors by Srb10 cyclin-dependent kinase (2001)
Chi, Yong, Huddleston, Michael J., Zhang, Xiaolong, Young, Richard A., Annan, Roland S., Carr, Steven A., ...
Management of risks for physical educators / (1998)
Thesis (M.S.)--St. Cloud State University, 1998.
Michael J. Huddleston, S. Annan, Mark F. Bean, Steven A. Carr
A mass spectrometry-based method that does not involve the use of radioIabeling was developed for selective detection of phosphopeptides in complex mixtures. Mixtures of phosphorylated and...
S. A. Carr, M. J. Huddleston, M. F. Bean, Email Alerting, Steven A. Carr, Michael J. Huddleston, ...
Selective identification and differentiation of N- and O-linked oligosaccharides in glycoproteins by liquid chromatography-mass spectrometry
References Email alerting service Notes Downloaded from (1992)
S. A. Carr, M. J. Huddleston, M. F. Bean, Steven A. Carr, Michael J. Huddleston, ...
Selective identification and differentiation of N- and O-linked oligosaccharides in glycoproteins by liquid chromatography-mass spectrometry
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 1981.
Design of potent and selective human cathepsin K inhibitors that span the active site
Thompson, Scott K., Halbert, Stacie M., Bossard, Mary J., Tomaszek, Thaddeus A., Levy, Mark A., Zhao, Baoguang, ...
Potent and selective active-site-spanning inhibitors have been designed for cathepsin K, a cysteine protease unique to osteoclasts. They act by mechanisms that involve tight binding intermediates,...
Negative regulation of Gcn4 and Msn2 transcription factors by Srb10 cyclin-dependent kinase
Chi, Yong, Huddleston, Michael J., Zhang, Xiaolong, Young, Richard A., Annan, Roland S., Carr, Steven A., ...
The budding yeast transcriptional activator Gcn4 is rapidly degraded in an SCFCdc4-dependent manner in vivo. Upon fractionation of yeast extracts to identify factors that mediate Gcn4 ubiquitination,...
Design of potent and selective human cathepsin K inhibitors that span the active site
Thompson, Scott K., Halbert, Stacie M., Bossard, Mary J., Tomaszek, Thaddeus A., Levy, Mark A., Zhao, Baoguang, ...
Potent and selective active-site-spanning inhibitors have been designed for cathepsin K, a cysteine protease unique to osteoclasts. They act by mechanisms that involve tight binding intermediates,...
Negative regulation of Gcn4 and Msn2 transcription factors by Srb10 cyclin-dependent kinase
Chi, Yong, Huddleston, Michael J., Zhang, Xiaolong, Young, Richard A., Annan, Roland S., Carr, Steven A., ...
The budding yeast transcriptional activator Gcn4 is rapidly degraded in an SCFCdc4-dependent manner in vivo. Upon fractionation of yeast extracts to identify factors that mediate Gcn4 ubiquitination,...
Proteomic screen defines the Polo-box domain interactome and identifies Rock2 as a Plk1 substrate
Lowery, Drew M, Clauser, Karl R, Hjerrild, Majbrit, Lim, Dan, Alexander, Jes, Kishi, Kazuhiro, ...
Polo-like kinase-1 (Plk1) phosphorylates a number of mitotic substrates, but the diversity of Plk1-dependent processes suggests the existence of additional targets. Plk1 contains a specialized...
Shaham, Oded, Wei, Ru, Wang, Thomas J, Ricciardi, Catherine, Lewis, Gregory D, Vasan, Ramachandran S, ...
Glucose ingestion after an overnight fast triggers an insulin-dependent, homeostatic program that is altered in diabetes. The full spectrum of biochemical changes associated with this transition is...
Lewis, Gregory D., Wei, Ru, Liu, Emerson, Yang, Elaine, Shi, Xu, Martinovic, Maryann, ...
Emerging metabolomic tools have created the opportunity to establish metabolic signatures of myocardial injury. We applied a mass spectrometry–based metabolite profiling platform to 36 patients...
Burkard, Mark E., Maciejowski, John, Rodriguez-Bravo, Verónica, Repka, Michael, Lowery, Drew M., Clauser, Karl R., ...
Self-regulated movement of Polo-like kinase 1 to the midzone of the mitotic spindle initiates a local signaling cascade that activates the cell division machinery at the cell's equator.
Identifying the proteins to which small-molecule probes and drugs bind in cells
Ong, Shao-En, Schenone, Monica, Margolin, Adam A., Li, Xiaoyu, Do, Kathy, Doud, Mary K., ...
Most small-molecule probes and drugs alter cell circuitry by interacting with 1 or more proteins. A complete understanding of the interacting proteins and their associated protein complexes, whether...
PEPPeR: A Platform for Experimental Proteomic Pattern Recognition
Jaffe, Jacob D., Mani, D. R., Leptos, Kyriacos C., Church, George M., Gillette, Michael A., Carr, Steven A.
Jaffe, Jacob D., Keshishian, Hasmik, Chang, Betty, Addona, Theresa A., Gillette, Michael A., Carr, Steven A.
Verification of candidate biomarker proteins in blood is typically done using multiple reaction monitoring (MRM) of peptides by LC-MS/MS on triple quadrupole MS systems. MRM assay development for...