Steven A. Short

Nucleotide sequence of the CytR regulatory gene of E. coli K-12 (1986)

Valentin-Hansen, Poul, Larsen, Jens Erik Løve, Højrup, Peter, Short, Steven A., Barbier, Claire S.

We have determined the nucleotide sequence of the cytR gene, which codes for the Cyt repressor (CytR). The coding region consists of 1023 or 1029 bp. The subunits of CytR are thus predicted to...

Anatomy of a proficient enzyme: The structure of orotidine 5′-monophosphate decarboxylase in the presence and absence of a potential transition state analog

Miller, Brian G., Hassell, Anne M., Wolfenden, Richard, Milburn, Michael V., Short, Steven A.

Orotidine 5′-phosphate decarboxylase produces the largest rate enhancement that has been reported for any enzyme. The crystal structure of the recombinant Saccharomyces cerevisiae enzyme has been...

Metabolism of Phosphatidylglycerol, Lysylphosphatidylglycerol, and Cardiolipin of Staphylococcus aureus

Short, Steven A., White, David C.

Staphylococcus aureus accumulated cardiolipin (CL) and lost phosphatidylglycerol (PG) during the stationary phase of growth. The minor lipids, phosphatidylethanolamine and phosphatidylglucose, also...

Metabolism of the Glucosyl Diglycerides and Phosphatidylglucose of Staphylococcus aureus

Short, Steven A., White, David C.

A glucose containing lipid, phosphatidylglucose (probably 3-sn-phosphatidyl-1′-glucose) and a lipid tentatively identified as phosphatidylethanolamine have been characterized in the lipids of...

Phospholipid Metabolism in Ferrobacillus ferrooxidans

Short, Steven A., White, David C., Aleem, M. I. H.

The lipid composition of the chemoautotroph Ferrobacillus ferrooxidans has been examined. Fatty acids represent 2% of the dry weight of the cells and 86% of the total are extractable with organic...

Biosynthesis of Cardiolipin from Phosphatidylglycerol in Staphylococcus aureus

Short, Steven A., White, David C.

Cardiolipin (CL) synthetase from Staphylococcus aureus catalyzes the complete conversion of two molecules of phosphatidylglycerol (PG) to one molecule of CL and one molecule of glycerol. The fatty...

D-Lactate Dehydrogenase Binding in Escherichia coli dld- Membrane Vesicles Reconstituted for Active Transport*

Short, Steven A., Kaback, H. Ronald, Kohn, Leonard D.

When membrane vesicles prepared from a D-lactate dehydrogenase mutant of E. coli ML 308-225 are treated with a homogeneous preparation of D-lactate dehydrogenase, the enzyme binds to the vesicles and...

Fourier transform ion cyclotron resonance MS reveals the presence of a water molecule in an enzyme transition-state analogue complex

Borchers, Christoph H., Marquez, Victor E., Schroeder, Gottfried K., Short, Steven A., Snider, Mark J., Speir, J. Paul, ...

The structures of several powerful inhibitors of hydrolytic enzymes resemble that of the altered substrate in the transition state, except that a hydrogen atom replaces one substituent (typically the...

Antiviral Activity of GW678248, a Novel Benzophenone Nonnucleoside Reverse Transcriptase Inhibitor

Ferris, Robert G., Hazen, Richard J., Roberts, Grace B., St. Clair, Marty H., Chan, Joseph H., Romines, Karen R., ...

The compound GW678248 is a novel benzophenone nonnucleoside reverse transcriptase inhibitor (NNRTI). Preclinical assessment of GW678248 indicates that this compound potently inhibits wild-type (WT)...

Anatomy of a proficient enzyme: The structure of orotidine 5′-monophosphate decarboxylase in the presence and absence of a potential transition state analog

Miller, Brian G., Hassell, Anne M., Wolfenden, Richard, Milburn, Michael V., Short, Steven A.

Orotidine 5′-phosphate decarboxylase produces the largest rate enhancement that has been reported for any enzyme. The crystal structure of the recombinant Saccharomyces cerevisiae enzyme has been...

Metabolism of Phosphatidylglycerol, Lysylphosphatidylglycerol, and Cardiolipin of Staphylococcus aureus

Short, Steven A., White, David C.

Staphylococcus aureus accumulated cardiolipin (CL) and lost phosphatidylglycerol (PG) during the stationary phase of growth. The minor lipids, phosphatidylethanolamine and phosphatidylglucose, also...

Metabolism of the Glucosyl Diglycerides and Phosphatidylglucose of Staphylococcus aureus

Short, Steven A., White, David C.

A glucose containing lipid, phosphatidylglucose (probably 3-sn-phosphatidyl-1′-glucose) and a lipid tentatively identified as phosphatidylethanolamine have been characterized in the lipids of...

Phospholipid Metabolism in Ferrobacillus ferrooxidans

Short, Steven A., White, David C., Aleem, M. I. H.

The lipid composition of the chemoautotroph Ferrobacillus ferrooxidans has been examined. Fatty acids represent 2% of the dry weight of the cells and 86% of the total are extractable with organic...

Biosynthesis of Cardiolipin from Phosphatidylglycerol in Staphylococcus aureus

Short, Steven A., White, David C.

Cardiolipin (CL) synthetase from Staphylococcus aureus catalyzes the complete conversion of two molecules of phosphatidylglycerol (PG) to one molecule of CL and one molecule of glycerol. The fatty...

D-Lactate Dehydrogenase Binding in Escherichia coli dld- Membrane Vesicles Reconstituted for Active Transport*

Short, Steven A., Kaback, H. Ronald, Kohn, Leonard D.

When membrane vesicles prepared from a D-lactate dehydrogenase mutant of E. coli ML 308-225 are treated with a homogeneous preparation of D-lactate dehydrogenase, the enzyme binds to the vesicles and...

Fourier transform ion cyclotron resonance MS reveals the presence of a water molecule in an enzyme transition-state analogue complex

Borchers, Christoph H., Marquez, Victor E., Schroeder, Gottfried K., Short, Steven A., Snider, Mark J., Speir, J. Paul, ...

The structures of several powerful inhibitors of hydrolytic enzymes resemble that of the altered substrate in the transition state, except that a hydrogen atom replaces one substituent (typically the...

Antiviral Activity of GW678248, a Novel Benzophenone Nonnucleoside Reverse Transcriptase Inhibitor

Ferris, Robert G., Hazen, Richard J., Roberts, Grace B., St. Clair, Marty H., Chan, Joseph H., Romines, Karen R., ...

The compound GW678248 is a novel benzophenone nonnucleoside reverse transcriptase inhibitor (NNRTI). Preclinical assessment of GW678248 indicates that this compound potently inhibits wild-type (WT)...