Schaller, Matthew A., Neupane, Rupak, Rudd, Brian D., Kunkel, Steven L., Kallal, Lara E., Lincoln, Pamela, ...
Recent data have indicated that an important instructive class of signals regulating the immune response is Notch ligand-mediated activation. Using quantitative polymerase chain reaction, we observed...
Hogaboam, Cory M., Blease, K., Kunkel, Steven L.
Fungus-induced asthmatic disease is characterized by persistent airway hyperreactivity and remodeling.¶ Objective and design: To determine the role of IL-18 in the allergic airway response to...
Matsukawa, Akihiro, Lukacs, Nicholas W., Hogaboam, Cory M., Chensue, Stephen W., Kunkel, Steven L.
Chemokines constitute a large family of chemotactic cytokines that belong to a super-gene family of 8–10 kDa proteins. The chemokines are considered to be primarily beneficial in host defense...
Chemokines and their role in airway hyper-reactivity (2000)
Blease, Kate, Lukacs, Nicholas W, Hogaboam, Cory M, Kunkel, Steven L
Abstract Airway hyper-reactivity is a characteristic feature of many inflammatory lung diseases and is defined as an exaggerated degree of airway narrowing. Chemokines and their receptors are...
Hogaboam, Cory M., Blease, K., Kunkel, Steven L.
Objective and Design: This study examined he role of nitric oxide in changes in airway physiology and inflammation in a murine model of fungal allergy induced by Aspergillus fumigatus (A. fumigatus)...
Macrophage infiltration in human non-small-cell lung cancer: the role of CC chemokines (2000)
DiGiovine, Bruno, Burdick, Marie D., Arenberg, Douglas A., Kunkel, Steven L., Strieter, Robert M., Strom, Scott R. B., ...
Bronchogenic carcinoma is the leading cause of malignancy-related mortality in the United States, with an overall 5-year survival rate of less than 15%. This aggressive behavior reflects, among...
Chemokines in rheumatoid arthritis (1998)
Kunkel, Steven L., Szekanec, Zoltan, Koch, Alisa E., Strieter, Robert M.
Peer Reviewed
Kunkel, Steven L., Chensue, Stephen W., Warmington, K., Ruth, Jeffrey H.
Objective and Design: This study examines the local and regional effects of exogenously administered interleukins 10 (IL-10) and 12 (IL-12) on pulmonary granulomas mediated by Th1/type 1-(IFN-) and...
Nickoloff, Brian J., Fivenson, David P., Kunkel, Steven L., Strieter, Robert M., Turka, Laurence A.
Despite the highly diverse reaction patterns of benign and malignant skin diseases involving T lymphocytes, polymerase chain reaction analysis of cytokine mRNAs present in biopsy samples has revealed...
Orens, Jonathan B., Lukacs, Nicholas W., Kunkel, Steven L., Burdick, Marie D., Wilke, Carol A., Walz, Alfred, ...
Previous studies have shown that during the development of a mixed lymphocyte reaction (MLR) levels of the chemotactic cytokines IL-8 and MCP-1 (members of the C-X-C and C-C supergene families,...
Wei, Ru-Qi, Lin, Hua, Chen, Gwo-Hsiao, Beer, David G., Kunkel, Steven L., Bolling, Steven F.
Tumor necrosis factor-[alpha] (TNF) is a multifunctional cytokine involved in the immunopathologic consequences of allograft rejection. We have previously demonstrated that anti-TNF antibody...
Lukacs, Nicholas W., Strieter, Robert M., Evanoff, Holly L., Burdick, Marie D., Kunkel, Steven L.
The allogeneic mixed lymphocyte reaction (MLR) is regarded as an effective model for examining the events which occur during an allospecific immune response. Numerous studies have delineated the role...
The role of chemokines in Schistosoma mansoni granuloma formation (1994)
Lukacs, Nicholas W., Kunkel, Steven L., Strieter, Robert M., Chensue, Stephen W.
The eradication of parasitic diseases, such as schistosomiasis, has been the focus of investigations worldwide for many decades. However, attempts to control their continual spread have, at best,...
Donnelly, S. C., Haslett, C., Strieter, Robert M., Kunkel, Steven L., Walz, Alfred, Robertson, C. R., ...
Neutrophils have been implicated in the pathogenesis of the adult respiratory distress syndrome (ARDS). We have measured concentrations of the neutrophil attractant interleukin-8 in blood and...
Koch, Alisa E., Kunkel, Steven L., Chensue, Stephen W., Haines, G. Kenneth, Strieter, Robert M.
Interleukin-1 (IL-1) has protean effects in the pathogenesis of rheumatoid arthritis (RA). These effects include production of prostaglandins and collagenase from rheumatoid fibroblasts as well as...
Elner, Victor M., Scales, Wendy E., Elner, Susan G., Danforth, Jean, Kunkel, Steven L., Strieter, Robert M.
Retinal and choroidal inflammatory lesions are important causes of visual loss, but the mechanisms regulating intraocular inflammation remain poorly understood. By virtue of its position at the...
Role of CC chemokine CCL6/C10 as a monocyte chemoattractant in a murine acute peritonitis (1992)
Andrew M. LaFleur, Nicholas W. Lukacs, Steven L. Kunkel, Akihiro Matsukawa
THE aim of this study was to determine the role of CC chemokine CCL6/C10 in acute inflammation. Intraperitoneal injection of thioglycollate increased peritoneal CCL6, which peaked at 4 h and remained...
Guice, K. S., Oldham, Keith T., Remick, Daniel G., Kunkel, Steven L., Ward, Peter A.
The pathogenesis of acute pancreatitis is incompletely defined, but the outcome is determined in part by an acute inflammatory process. Pancreatitis-associated inflammation appears to play a role in...
Basha, Michael A., Meyer, G. Scott, Kunkel, Steven L., Strieter, Robert M., Rivers, Emanuel P.
Tumor necrosis factor-alpha (TNF) was measured in 21 patients who presented to the emergency department in normothermic, nontraumatic cardiopulmonary arrest and who had eventual return of spontaneous...
Thornton, Amanda J., Ham, John, Kunkel, Steven L.
Communication circuits operating between activated monocytes/macrophages and adjacent hepatocytes in the liver effect important alterations in hepatocyte function. We demonstrate here that primary...
Krasnow, Stephanie W., Zhang, Liqian, Leung, Kwanyee, Osborn, Laurelee, Kunkel, Steven L., Nabel, Gary J.
Gene expression in eukaryotic cells can be altered in different ways by extracellular agents, including mitogens and cytokines. Such differential gene expression is mediated in part through the...
The role of TNF in diverse pathologic processes (1991)
Kunkel, Steven L., Strieter, Robert M., Chensue, Stephen W., Campbell, Darrell A., Remick, Daniel G.
Peer Reviewed
DeMarco, Deborah, Kunkel, Steven L., Strieter, Robert M., Basha, Michael A., Zurier, Robert B.
We report here that human synovial cells stimulated by interleukin-1[alpha] and interleukin-1[beta] express mRNA for both IL-8 (neutrophil chemotactic peptide) and monocyte chemotactic protein. IL-1...
Human neutrophils exhibit disparate chemotactic factor gene expression (1990)
Strieter, Robert M., Kasahara, K., Allen, R., Showell, H.J., Standiford, T.J., Kunkel, Steven L.
The evolution of acute inflammation from initiation through resolution is associated with the changing character of the infiltrating leukocytes. Recruitment of these leukocytes is dependent upon the...
Standiford, T. J., Strieter, Robert M., Kasahara, K., Kunkel, Steven L.
The role(s) of novel chemotactic cytokines as mediators in the pathogenesis of many acute and chronic disease states is becoming increasingly apparent. One of these cytokines, interleukin 8, is a...
Disparate gene expression of chemotactic cytokines by human mononuclear phagocytes (1990)
Strieter, Robert M., Chensue, Stephen W., Standiford, T. J., Basha, Michael A., Showell, H. J., Kunkel, Steven L.
Chemotactic cytokines are becoming increasingly recognized as important participants in the coordinate recruitment of specific inflammatory cells. In this manuscript we present data demonstrating...
Strieter, Robert M., Wiggins, R., Phan, Sem H., Wharram, B. L., Showell, H. J., Remick, Daniel G., ...
A number of cytokines are active during the evolution of an inflammatory response, including tumor necrosis factor-[alpha], interleukin-1, and novel chemotactic cytokines. This latter group of...
Cyclosporine a inhibits TNF production without decreasing TNF mRNA levels (1989)
Remick, Daniel G., Nguyen, Dung T., Eskandari, Mark K., Strieter, Robert M., Kunkel, Steven L.
The role of cytokines in health and disease has received increasing attention and numberous investigations have explored the regulation of cytokine gene expression. Tumor necrosis factor-[alpha]...
The beta form is the dominant interleukin released by murine peritoneal macrophages (1989)
Chensue, Stephen W., Shmyr-Forsch, C., Otterness, Ivan G., Kunkel, Steven L.
Using highly specific polyclonal antisera raised against recombinant murine IL-1 [alpha] and [beta], we performed solid-phase immunoabsorption studies on supernates of resident and adjuvant-elicited...
Strieter, Robert M., Kunkel, Steven L., Showell, H.J., Marks, Rory M.
Monokines have been increasingly recognized as communication signals that interact with both immune and non-immune cells during inflammation. Specifically, interleukin-1alpha (IL-lalpha),...
Cellular and molecular regulation of tumor necrosis factor-alpha production by pentoxofylline (1988)
Strieter, Robert M., Remick, Daniel G., Ward, Peter A., Spengler, Robert N., Lynch III, J.P., Larrick, James W., ...
Tumor necrosis factor-alpha (TNF), a mononuclear phagocyte (MO)-derived peptide, is increasingly being recognized for its pleomorphic immunologic effects. A number of investigations have demonstrated...
The Role of Tumor Necrosis Factor and Interleukin 1 in the Immunoinflammatory Response (1988)
Larrick, James W., Kunkel, Steven L.
Monocytes and tissue macrophages produce at least two groups of protein mediators of inflammation, interleukin 1 (IL-1) and tumor necrosis factor (TNF). Recent studies have emphasized that TNF and...
Remick, Daniel G., Larrick, James W., Nguyen, Dung T., Kunkel, Steven L.
Interleukin 2 (IL-2) is a potent lymphokine involved in the regulation of immune responses and is classically regarded as a stimulus for the activation and growth of T-cells. Recent reports have...
Arachidonic acid metabolism is altered in sarcoid alveolar macrophages, (1987)
Bachwich, Peter R., Kunkel, Steven L.
Macrophages produce various arachidonic acid (AA) metabolites which may either enhance or suppress inflammatory processes. We investigated AA metabolite production by alveolar macrophages (AMs) from...
Tumor necrosis factor-induced alterations in circulating leukocyte populations (1986)
Remick, Daniel G., Larrick, James W., Kunkel, Steven L.
Tumor necrosis factor is a potent agent possessing diverse biological functions. We investigated the effects of intravenous administration of human recombinant tumor necrosis factor (TNF) on immune...
Reduction of myocardial infarct size by neutrophil depletion: Effect of duration of occlusion (1986)
Jolly, Stanley R., Kane, William J., Hook, Bruce G., Abrams, Gerald D., Kunkel, Steven L., Lucchesi, Benedict Robert
Experiments were performed in the dog to examine the effects of neutropenia on ultimate infarct size resulting from short (90 minutes) or prolonged (4 hours) circumflex coronary artery occlusion....
Regulation of macrophage tumor necrosis factor production by prostaglandin E2 (1986)
Kunkel, Steven L., Wiggins, R. C., Chensue, Stephen W., Larrick, James W.
We have studied the role of prostaglandin E2 on the modulation of tumor necrosis factor by immunologically elicited and lipopolysaccharide treated murine macrophages. Indomethacin, a potent inhibitor...
Bachwich, Peter R., Chensue, Stephen W., Larrick, James W., Kunkel, Steven L.
We have investigated the effect of tumor necrosis factor on the release of interleukin-1 and PGE2 from murine resident peritoneal macrophages. Tumor necrosis factor causes an increase in the...
Kunkel, Steven L., Chensue, Stephen W., Higashi, Gene I.
The expression of Ia antigen by various murine and human macrophage populations and the ability of prostaglandins of the E series to regulate Ia antigen expression were explored. Monocytes and...
Platelet depletion in experimental myocardial infarction (1985)
Kunkel, Steven L., Lucchesi, Benedict Robert, Liddicoat, J., Jolly, Stanley R., Abrams, Gerald D., Schumacher, William A.
Accumulation of platelets in the microvasculature after acute myocardial ischemia may exacerbate tissue injury through the formation of microthrombi and by the release of vasoactive substances. To...
Arachidonic acid metabolites regulate interleukin-1 production (1985)
Kunkel, Steven L., Chensue, Stephen W.
We have investigated the role of arachidonic acid metabolites in the regulation of interleukin-1 production by murine peritoneal macrophages. Indomethacin a potent inhibitor of prostaglandin...
Role of oxygen reactive species in egg-induced granulomatous inflammation (1984)
Chensue, Stephen W., Quinlan, L., Higashi, G. I., Kunkel, Steven L.
The role of oxygen reactive species in granulomatous hypersensitivity was explored using a model of pulmonary granulomas induced by intravenous injection of eggs from the parasite . Macrophages from...
Kunkel, Steven L., Duque, Ricardo E.
Mononuclear phagocytes are undoubtedly the sine qua non of chronic inflammatory reactions. This is demonstrated by their unique ability to function as phagocytic, secretory, or effector cells during...
Artificial anterior chamber for the growing of membranes on lens implants (1983)
Kunkel, Steven L., Wolter, J. Reimer
Intraocular lens implants made of glass and plastic were placed into the lumen of short pieces of clear plastic tubing. These were incompletely closed at both ends with nylon sutures, filled with...
Kunkel, Steven L., Manderino, George L., Marasco, Wayne A., Kaercher, Kip, Hirata, Arthur A., Ward, Peter A.
An enzyme-linked immunosorbent assay has been developed to detect human C5a antigen. This ELISA methodology has been shown to be a highly sensitive technique capable of detecting C5a antigen...
Abdominal implantation of intraocular lenses resulting in the formation of reactive membranes (1983)
Wolter, J. Reimer, Kunkel, Steven L.
For a better understanding of the cytological principles involved in the formation of reactive membranes on lens implants in the human eye, two kinds of modern implants were placed into the...
Kunkel, Steven L., Kaercher, Kip, Plewa, Michael C., Fantone, Joseph C., Ward, Peter A.
Mononuclear phagocytes are knwon to play a key role in various phlogistic reactions by synthesizing and releasing products that may potentiate or inhibit inflammatory processes. The expression of...
Kunkel, Steven L., Plewa, Michael C., Fantone, Joseph C., Ward, Peter A.
Peer Reviewed
Fantone, Joseph C., Kunkel, Steven L., Weingarten, Brenda
Peer Reviewed
Purification of human C5a des arg by immunoadsorbent and molecular sieve chromatography (1982)
Manderino, George L., Suarez, Adoracion F., Kunkel, Steven L., Ward, Peter A., Hirata, Arthur A., Showell, Henry
Human C5a des arg was isolated from complement-activated serum by immunoadsorption followed by Sephadex G-75 chromatography. C5a des arg obtained by this 2-step procedure was shown to be...
Suppression of acute and chronic inflammation by orally administered prostaglandins (1981)
Kunkel, Steven L., Ogawa, Hiroshi, Conran, Philip B., Ward, Peter A., Zurier, Robert B.
Oral administration of a stable analog of prostaglandin E, (PGE 1 ), 15-(S)-15-methyl-prostaglandin E 1 , can suppress both chronic adjuvant-induced polyarthritis and acute immune complex-induced...
Fantone, Joseph C., Kunkel, Steven L., Ward, Peter A., Zurier, Robert B.
In two of three patients with peripheral vascular disease, systemic infusion of PGE1 inhibited chemotactic factor induced secretion of glucosaminidase from neutrophils.
Chemokines and their role in airway hyper-reactivity
Blease, Kate, Lukacs, Nicholas W, Hogaboam, Cory M, Kunkel, Steven L
Airway hyper-reactivity is a characteristic feature of many inflammatory lung diseases and is defined as an exaggerated degree of airway narrowing. Chemokines and their receptors are involved in...
Jedrzkiewicz, Sean, Kataeva, Galina, Hogaboam, Cory M., Kunkel, Steven L., Strieter, Robert M., McKay, Derek M.
Bacterial superantigens (SAgs) have been implicated in inflammatory disease, and SAg-treated mice have increased jejunal T cells. Here we show that T84 cells (a human epithelial cell line) display...
Hogaboam, Cory M., Steinhauser, Matthew L., Schock, Harold, Lukacs, Nicholas, Strieter, Robert M., Standiford, Theodore, ...
This study demonstrates that the therapeutic effect of a nitric oxide inhibitor in a murine model of fecal peritonitis is mediated in part by increased levels of interleukin-10 (IL-10) and monocyte...
Elevated Chemokine Responses Are Maintained in Lungs after Clearance of Viral Infection
Weinberg, Jason B., Lutzke, Mary L., Efstathiou, Stacey, Kunkel, Steven L., Rochford, Rosemary
We observed two patterns of chemokine expression in the lungs of mice infected with murine gammaherpesvirus 68: peaks of chemokine expression correlated with or occurred after the peak of viral gene...
Critical role for the chemokine MCP-1/CCR2 in the pathogenesis of bronchiolitis obliterans syndrome
Belperio, John A., Keane, Michael P., Burdick, Marie D., Lynch, Joseph P., Xue, Ying Ying, Berlin, Aaron, ...
Bronchiolitis obliterans syndrome (BOS) is the major limitation to survival after lung transplantation. Acute rejection, its main risk factor, is characterized by perivascular/bronchiolar leukocyte...
Factors Affecting Release of Heat-Labile Enterotoxin by Enterotoxigenic Escherichia coli
Kunkel, Steven L., Robertson, Donald C.
Various conditions affecting the release of heat-labile enterotoxin (LT) by enterotoxigenic Escherichia coli have been examined. The pH of a defined medium containing three amino acids, M-9 salts,...
Kunkel, Steven L., Robertson, Donald C.
Heat-labile enterotoxin (LT) produced by a human strain of enterotoxigenic Escherichia coli (286C2) was purified to homogeneity from pH extracts of fermentor-grown cells by ultrafiltration, (NH4)2SO4...
Zeng, Xianying, Moore, Thomas A., Newstead, Michael W., Deng, Jane C., Kunkel, Steven L., Luster, Andrew D., ...
CXC chemokines that lack the ELR motif, including interferon-inducible protein 10 [IP-10 (CXCL10)] and monokine induced by gamma interferon (IFN-γ) [MIG (CXCL9)], have been shown to mediate the...
Chemokines and their role in airway hyper-reactivity
Blease, Kate, Lukacs, Nicholas W, Hogaboam, Cory M, Kunkel, Steven L
Airway hyper-reactivity is a characteristic feature of many inflammatory lung diseases and is defined as an exaggerated degree of airway narrowing. Chemokines and their receptors are involved in...
Jedrzkiewicz, Sean, Kataeva, Galina, Hogaboam, Cory M., Kunkel, Steven L., Strieter, Robert M., McKay, Derek M.
Bacterial superantigens (SAgs) have been implicated in inflammatory disease, and SAg-treated mice have increased jejunal T cells. Here we show that T84 cells (a human epithelial cell line) display...
Hogaboam, Cory M., Steinhauser, Matthew L., Schock, Harold, Lukacs, Nicholas, Strieter, Robert M., Standiford, Theodore, ...
This study demonstrates that the therapeutic effect of a nitric oxide inhibitor in a murine model of fecal peritonitis is mediated in part by increased levels of interleukin-10 (IL-10) and monocyte...
Elevated Chemokine Responses Are Maintained in Lungs after Clearance of Viral Infection
Weinberg, Jason B., Lutzke, Mary L., Efstathiou, Stacey, Kunkel, Steven L., Rochford, Rosemary
We observed two patterns of chemokine expression in the lungs of mice infected with murine gammaherpesvirus 68: peaks of chemokine expression correlated with or occurred after the peak of viral gene...
Critical role for the chemokine MCP-1/CCR2 in the pathogenesis of bronchiolitis obliterans syndrome
Belperio, John A., Keane, Michael P., Burdick, Marie D., Lynch, Joseph P., Xue, Ying Ying, Berlin, Aaron, ...
Bronchiolitis obliterans syndrome (BOS) is the major limitation to survival after lung transplantation. Acute rejection, its main risk factor, is characterized by perivascular/bronchiolar leukocyte...