Development of a Universal Intimin Antiserum and PCR Primers
Batchelor, Miranda, Knutton, Stuart, Caprioli, Alfredo, Huter, Veronika, Zanial, Mazlina, Dougan, Gordon, ...
Enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli (EHEC) constitute a significant risk to human health worldwide. A hallmark of both pathogens is their ability to produce...
Daniell, Sarah J., Delahay, Robin M., Shaw, Robert K., Hartland, Elizabeth L., Pallen, Mark J., Booy, Frank, ...
Many animal and plant pathogens use type III secretion systems to secrete key virulence factors, some directly into the host cell cytosol. However, the basis for such protein translocation has yet to...
Adu-Bobie, Jeannette, Frankel, Gad, Bain, Christopher, Goncalves, Azizedite Guedes, Trabulsi, Luiz R., Douce, Gill, ...
Intimins are outer membrane proteins expressed by enteric bacterial pathogens capable of inducing intestinal attachment-and-effacement lesions. A eukaryotic cell-binding domain is located within a...
Bain, Christopher, Keller, Rogeria, Collington, Georgina K., Trabulsi, Luiz R., Knutton, Stuart
Elevated concentrations of intracellular calcium ([Ca]i) have been implicated as an important signalling event during attaching and effacing (A/E) lesion formation by enteropathogenic Escherichia...
Collington, Georgina K., Booth, Ian W., Donnenberg, Michael S., Kaper, James B., Knutton, Stuart
The pathophysiology of enteropathogenic Escherichia coli (EPEC) diarrhea remains uncertain. In vitro, EPEC stimulates a rapid increase in short-circuit current (Isc) across Caco-2 cell monolayers...
Afa, a Diffuse Adherence Fibrillar Adhesin Associated with Enteropathogenic Escherichia coli
Keller, Rogéria, Ordoñez, Juana G., De Oliveira, Rosana R., Trabulsi, Luiz R., Baldwin, Thomas J., Knutton, Stuart
O55 is one of the most frequent enteropathogenic Escherichia coli (EPEC) O serogroups implicated in infantile diarrhea in developing countries. Multilocus enzyme electrophoresis analysis showed that...
Polymorphisms within EspA Filaments of Enteropathogenic and Enterohemorrhagic Escherichia coli
Neves, Bianca C., Shaw, Robert K., Frankel, Gad, Knutton, Stuart
Enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli (EHEC) possess a filamentous type III secretion system (TTSS) employed to deliver effector proteins into host cells. EspA is a...
Neves, Bianca C., Mundy, Rosanna, Petrovska, Liljana, Dougan, Gordon, Knutton, Stuart, Frankel, Gad
Enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli are extracellular pathogens that employ a type III secretion system to export translocator and effector proteins, proteins which...
Transcriptome of Enterohemorrhagic Escherichia coli O157 Adhering to Eukaryotic Plasma Membranes
Dahan, Sivan, Knutton, Stuart, Shaw, Robert K., Crepin, Valerie F., Dougan, Gordon, Frankel, Gad
Using a DNA microarray, we determined changes in enterohemorrhagic Escherichia coli O157:H7 gene expression during binding to plasma membranes. Analysis of the complete transcriptomes of the bound...
Zhang, Lihong, Chaudhuri, Roy R., Constantinidou, Chrystala, Hobman, Jon L., Patel, Mala D., Jones, Antony C., ...
Enterohemorrhagic Escherichia coli (EHEC) O157:H7 subverts host cells through a type III secretion system encoded by the locus for enterocyte effacement (LEE). Genome sequencing of this pathotype...
Dahan, Sivan, Wiles, Siouxsie, La Ragione, Roberto M., Best, Angus, Woodward, Martin J., Stevens, Mark P., ...
Enterohemorrhagic Escherichia coli, enteropathogenic E. coli, and Citrobacter rodentium are highly adapted enteropathogens that successfully colonize their host's gastrointestinal tract via the...
Shaw, Robert K, Cleary, Jennifer, Murphy, Michael S., Frankel, Gad, Knutton, Stuart
Enteropathogenic Escherichia coli (EPEC) strains deliver effector proteins Tir, EspB, Map, EspF, EspH, and EspG into host cells to induce brush border remodeling and produce attaching and effacing...
Polarity of Enteropathogenic Escherichia coli EspA Filament Assembly and Protein Secretion
Crepin, Valérie F., Shaw, Robert, Abe, Cecilia M., Knutton, Stuart, Frankel, Gad
Type III secretion systems (TTSS) are sophisticated macromolecular structures that play an imperative role in bacterial infections and human disease. The TTSS needle complex is conserved among...
Identification of an Escherichia coli Operon Required for Formation of the O-Antigen Capsule
Peleg, Adi, Shifrin, Yulia, Ilan, Ophir, Nadler-Yona, Chen, Nov, Shani, Koby, Simi, ...
Escherichia coli produces polysaccharide capsules that, based on their mechanisms of synthesis and assembly, have been classified into four groups. The group 4 capsule (G4C) polysaccharide is...
Batchelor, Miranda, Guignot, Julie, Patel, Amit, Cummings, Nicola, Cleary, Jennifer, Knutton, Stuart, ...
While remaining extracellular, enteropathogenic Escherichia coli (EPEC) establish direct links with the cytoskeleton of the target epithelial cell leading to the formation of actin-rich pedestals...
Batchelor, Miranda, Guignot, Julie, Patel, Amit, Cummings, Nicola, Cleary, Jennifer, Knutton, Stuart, ...
Shaw, Robert K., Smollett, Katherine, Cleary, Jennifer, Garmendia, Junkal, Straatman-Iwanowska, Ania, Frankel, Gad, ...
Enteropathogenic Escherichia coli infection of intestinal epithelial cells leads to localized depletion of the microtubule cytoskeleton, an effect that is dependent on delivery of type III...
EspF of Enteropathogenic Escherichia coli Binds Sorting Nexin 9
Marchès, Oliver, Batchelor, Miranda, Shaw, Robert K., Patel, Amit, Cummings, Nicola, Nagai, Takeshi, ...
EspF of enteropathogenic Escherichia coli targets mitochondria and subverts a number of cellular functions. EspF consists of six putative Src homology 3 (SH3) domain binding motifs. In this study we...
Development of a Universal Intimin Antiserum and PCR Primers
Batchelor, Miranda, Knutton, Stuart, Caprioli, Alfredo, Huter, Veronika, Zanial, Mazlina, Dougan, Gordon, ...
Enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli (EHEC) constitute a significant risk to human health worldwide. A hallmark of both pathogens is their ability to produce...
Daniell, Sarah J., Delahay, Robin M., Shaw, Robert K., Hartland, Elizabeth L., Pallen, Mark J., Booy, Frank, ...
Many animal and plant pathogens use type III secretion systems to secrete key virulence factors, some directly into the host cell cytosol. However, the basis for such protein translocation has yet to...
Adu-Bobie, Jeannette, Frankel, Gad, Bain, Christopher, Goncalves, Azizedite Guedes, Trabulsi, Luiz R., Douce, Gill, ...
Intimins are outer membrane proteins expressed by enteric bacterial pathogens capable of inducing intestinal attachment-and-effacement lesions. A eukaryotic cell-binding domain is located within a...
Bain, Christopher, Keller, Rogeria, Collington, Georgina K., Trabulsi, Luiz R., Knutton, Stuart
Elevated concentrations of intracellular calcium ([Ca]i) have been implicated as an important signalling event during attaching and effacing (A/E) lesion formation by enteropathogenic Escherichia...
Collington, Georgina K., Booth, Ian W., Donnenberg, Michael S., Kaper, James B., Knutton, Stuart
The pathophysiology of enteropathogenic Escherichia coli (EPEC) diarrhea remains uncertain. In vitro, EPEC stimulates a rapid increase in short-circuit current (Isc) across Caco-2 cell monolayers...
Afa, a Diffuse Adherence Fibrillar Adhesin Associated with Enteropathogenic Escherichia coli
Keller, Rogéria, Ordoñez, Juana G., De Oliveira, Rosana R., Trabulsi, Luiz R., Baldwin, Thomas J., Knutton, Stuart
O55 is one of the most frequent enteropathogenic Escherichia coli (EPEC) O serogroups implicated in infantile diarrhea in developing countries. Multilocus enzyme electrophoresis analysis showed that...
Polymorphisms within EspA Filaments of Enteropathogenic and Enterohemorrhagic Escherichia coli
Neves, Bianca C., Shaw, Robert K., Frankel, Gad, Knutton, Stuart
Enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli (EHEC) possess a filamentous type III secretion system (TTSS) employed to deliver effector proteins into host cells. EspA is a...
Neves, Bianca C., Mundy, Rosanna, Petrovska, Liljana, Dougan, Gordon, Knutton, Stuart, Frankel, Gad
Enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli are extracellular pathogens that employ a type III secretion system to export translocator and effector proteins, proteins which...
Transcriptome of Enterohemorrhagic Escherichia coli O157 Adhering to Eukaryotic Plasma Membranes
Dahan, Sivan, Knutton, Stuart, Shaw, Robert K., Crepin, Valerie F., Dougan, Gordon, Frankel, Gad
Using a DNA microarray, we determined changes in enterohemorrhagic Escherichia coli O157:H7 gene expression during binding to plasma membranes. Analysis of the complete transcriptomes of the bound...
Zhang, Lihong, Chaudhuri, Roy R., Constantinidou, Chrystala, Hobman, Jon L., Patel, Mala D., Jones, Antony C., ...
Enterohemorrhagic Escherichia coli (EHEC) O157:H7 subverts host cells through a type III secretion system encoded by the locus for enterocyte effacement (LEE). Genome sequencing of this pathotype...
Dahan, Sivan, Wiles, Siouxsie, La Ragione, Roberto M., Best, Angus, Woodward, Martin J., Stevens, Mark P., ...
Enterohemorrhagic Escherichia coli, enteropathogenic E. coli, and Citrobacter rodentium are highly adapted enteropathogens that successfully colonize their host's gastrointestinal tract via the...
Shaw, Robert K, Cleary, Jennifer, Murphy, Michael S., Frankel, Gad, Knutton, Stuart
Enteropathogenic Escherichia coli (EPEC) strains deliver effector proteins Tir, EspB, Map, EspF, EspH, and EspG into host cells to induce brush border remodeling and produce attaching and effacing...
Polarity of Enteropathogenic Escherichia coli EspA Filament Assembly and Protein Secretion
Crepin, Valérie F., Shaw, Robert, Abe, Cecilia M., Knutton, Stuart, Frankel, Gad
Type III secretion systems (TTSS) are sophisticated macromolecular structures that play an imperative role in bacterial infections and human disease. The TTSS needle complex is conserved among...
Shaw, Robert K., Smollett, Katherine, Cleary, Jennifer, Garmendia, Junkal, Straatman-Iwanowska, Ania, Frankel, Gad, ...
Enteropathogenic Escherichia coli infection of intestinal epithelial cells leads to localized depletion of the microtubule cytoskeleton, an effect that is dependent on delivery of type III...
Identification of an Escherichia coli Operon Required for Formation of the O-Antigen Capsule
Peleg, Adi, Shifrin, Yulia, Ilan, Ophir, Nadler-Yona, Chen, Nov, Shani, Koby, Simi, ...
Escherichia coli produces polysaccharide capsules that, based on their mechanisms of synthesis and assembly, have been classified into four groups. The group 4 capsule (G4C) polysaccharide is...
Batchelor, Miranda, Guignot, Julie, Patel, Amit, Cummings, Nicola, Cleary, Jennifer, Knutton, Stuart, ...
While remaining extracellular, enteropathogenic Escherichia coli (EPEC) establish direct links with the cytoskeleton of the target epithelial cell leading to the formation of actin-rich pedestals...
Batchelor, Miranda, Guignot, Julie, Patel, Amit, Cummings, Nicola, Cleary, Jennifer, Knutton, Stuart, ...
EspF of Enteropathogenic Escherichia coli Binds Sorting Nexin 9
Marchès, Oliver, Batchelor, Miranda, Shaw, Robert K., Patel, Amit, Cummings, Nicola, Nagai, Takeshi, ...
EspF of enteropathogenic Escherichia coli targets mitochondria and subverts a number of cellular functions. EspF consists of six putative Src homology 3 (SH3) domain binding motifs. In this study we...
Bhakta, Sanjib, Besra, Gurdyal S., Upton, Anna M., Parish, Tanya, Gibson, Kevin J.C., ...
Mycolic acids represent a major component of the unique cell wall of mycobacteria. Mycolic acid biosynthesis is inhibited by isoniazid, a key frontline antitubercular drug that is inactivated by...
Singh, Mona P., Shaw, Robert K., Knutton, Stuart, Pallen, Mark J., Crepin, Valerie F., Frankel, Gad
Enteropathogenic Escherichia coli employs a filamentous type III secretion system, made by homopolymerization of the translocator protein EspA. In this study, we have shown that the N-terminal region...
Enterohemorrhagic Escherichia coli Exploits EspA Filaments for Attachment to Salad Leaves▿
Shaw, Robert K., Berger, Cedric N., Feys, Bart, Knutton, Stuart, Pallen, Mark J., Frankel, Gad
Enterohemorrhagic Escherichia coli (EHEC) strains are important food-borne pathogens that use a filamentous type III secretion system (fT3SS) for colonization of the gut epithelium. In this study we...
Shifrin, Yulia, Peleg, Adi, Ilan, Ophir, Nadler, Chen, Kobi, Simi, Baruch, Kobi, ...
Enterohemorrhagic and enteropathogenic Escherichia coli (EHEC and EPEC, respectively) strains represent a major global health problem. Their virulence is mediated by the concerted activity of an...