Suguru Takatsuto

Involvement of C-22-Hydroxylated Brassinosteroids in Auxin-Induced Lamina Joint Bending in Rice (2009)

Nakamura, Ayako, Fujioka, Shozo, Takatsuto, Suguru, Tsujimoto, Masafumi, Kitano, Hidemi, Yoshida, Shigeo, ...

The rice lamina joint is ideal material for investigating the activity of brassinosteroids (BRs) and auxin because of its high sensitivity to these compounds. Using a series of rice BR biosynthetic...

A Double Mutant for the CYP85A1 and CYP85A2 Genes of Arabidopsis Exhibits a Brassinosteroid Dwarf Phenotype (2008)

Kwon, Mi, Fujioka, Shozo, Jeon, Ji Hyun, Kim, Ho Bang, Takatsuto, Suguru, Yoshida, Shigeo, ...

Brassinosteroid (BR)-6-oxidases mediate the bridge reactions that connect the late and early C-6 oxidation pathways by converting 6-deoxoBR to 6-oxoBRs. Two similar genes of Arabidopsis, CYP85A1...

An Arabidopsis short root and dwarfism Mutant Defines a Novel Locus That Mediates Both Cell Division and Elongation (2008)

Lee, Hyun-Kyung, Kwon, Mi, Jeon, Ji-Hyun, Fujioka, Shozo, Kim, Ho-Bang, Park, So-Young, ...

Arabidopsis leaf morphology is determined by the coordinated action of cell division and elongation. Of all the hormones that control leaf shape, the brassinosteroids (BRs) are active components in...

Co-Regulation of Brassinosteroid Biosynthesis-Related Genes During Xylem Cell Differentiation (2007)

Yamamoto, Ryo, Fujioka, Shozo, Iwamoto, Kuninori, Demura, Taku, Takatsuto, Suguru, Yoshida, Shigeo, ...

To understand the regulatory mechanisms of brassinosteroid (BR) biosynthesis in specific plant developmental processes, we first investigated the accumulation profiles of BRs and sterols in xylem...

Brassinosteroid-6-oxidases from Arabidopsis and tomato catalyze multiple C-6 oxidations in brassinosteroid biosynthesis (2001)

Shimada, Yukihisa, Fujioka, Shozo, Miyauchi, Narumasa, Kushiro, Masayo, Takatsuto, Suguru, Nomura, Takahito, ...

Brassinosteroids (BRs) are steroidal plant hormones that are essential for growth and development. It has been proposed that BRs are synthesized via two parallel pathways, the early and late C-6...

Brassinolide and [26, 28-2H6]Brassinolide Are Differently Demethylated by Loss of C-26 and C-28, Respectively, in Marchantia polymorpha (2000)

Kim, Tae-Wuk, Chang, Soo Chul, Choo, Jongkil, Watanabe, Tsuyoshi, Takatsuto, Suguru, Yokota, Takao, ...

Metabolism of brassinolide in Marchantia polymorpha was investigated by use of in vivo suspension cultured cells. GC-MS analysis of metabolites derived from non-labelled brassinolide and [26,...

In Vivo and in Vitro Conversion of Teasterone to Typhasterol in Cultured Cells of Marchantia polymorpha (1999)

Park, Seung-Hyun, Han, Kwang-Seok, Kim, Tae-Wuk, Shim, Jae-Kuk, Takatsuto, Suguru, Yokota, Takao, ...

Identification by full-scan GC-MS revealed that [2H6]-teasterone fed to suspension cultured cells of Marchantia polymorpha was converted to [2H6]3-dehydroteasterone and [2H6]typhasterol. This...

Identification of Castasterone, 6-Deoxocastasterone, Typhasterol and 6-Deoxotyphasterol from the Shoots of Arabidopsis thaliana (1996)

Fujioka, Shozo, Choi, Yong-Hwa, Takatsuto, Suguru, Yokota, Takao, Li, Jianming, Chory, Joanne, ...

Endogenous brassinosteroids in the shoots of Arabidopsis thaliana were investigated. Castasterone, 6-deoxocastasterone, typhasterol and 6-deoxotyphasterol were identified by GC-MS. The co-occurrence...

The tomato DWARF enzyme catalyses C-6 oxidation in brassinosteroid biosynthesis

Bishop, Gerard J., Nomura, Takahito, Yokota, Takao, Harrison, Kate, Noguchi, Takahiro, Fujioka, Shozo, ...

Brassinosteroids (BRs) are steroidal plant hormones essential for normal plant growth and development. Mutants in the biosynthesis or perception of BRs are usually dwarf. The tomato Dwarf gene (D),...

BAS1: A gene regulating brassinosteroid levels and light responsiveness in Arabidopsis

Neff, Michael M., Nguyen, Serena M., Malancharuvil, Elizabeth J., Fujioka, Shozo, Noguchi, Takahiro, Seto, Hideharu, ...

The Arabidopsis bas1-D mutation suppresses the long hypocotyl phenotype caused by mutations in the photoreceptor phytochrome B (phyB). The adult phenotype of bas1-D phyB-4 double mutants mimics that...

Brassinosteroid/Sterol Synthesis and Plant Growth as Affected by lka and lkb Mutations of Pea1

Nomura, Takahito, Kitasaka, Yukiko, Takatsuto, Suguru, Reid, James B., Fukami, Motohiro, Yokota, Takao

The dwarf pea (Pisum sativum) mutants lka and lkb are brassinosteroid (BR) insensitive and deficient, respectively. The dwarf phenotype of the lkb mutant was rescued to wild type by exogenous...

The Arabidopsis dwarf1 Mutant Is Defective in the Conversion of 24-Methylenecholesterol to Campesterol in Brassinosteroid Biosynthesis1

Choe, Sunghwa, Dilkes, Brian P., Gregory, Brian D., Ross, Amanda S., Yuan, Heng, Noguchi, Takahiro, ...

Since the isolation and characterization of dwarf1-1 (dwf1-1) from a T-DNA insertion mutant population, phenotypically similar mutants, including deetiolated2 (det2), constitutive photomorphogenesis...

A Putative Role for the Tomato Genes DUMPY and CURL-3 in Brassinosteroid Biosynthesis and Response1

Koka, Chala V., Cerny, R. Eric, Gardner, Randy G., Noguchi, Takahiro, Fujioka, Shozo, Takatsuto, Suguru, ...

The dumpy (dpy) mutant of tomato (Lycopersicon esculentum Mill.) exhibits short stature, reduced axillary branching, and altered leaf morphology. Application of brassinolide and castasterone rescued...

Characterization of Brassinazole, a Triazole-Type Brassinosteroid Biosynthesis Inhibitor1

Asami, Tadao, Min, Yong Ki, Nagata, Noriko, Yamagishi, Kazutoshi, Takatsuto, Suguru, Fujioka, Shozo, ...

Screening for brassinosteroid (BR) biosynthesis inhibitors was performed to find chemicals that induce dwarfism in Arabidopsis, mutants that resembled BR biosynthesis mutants that can be rescued by...

Biosynthetic Pathways of Brassinolide in Arabidopsis1

Noguchi, Takahiro, Fujioka, Shozo, Choe, Sunghwa, Takatsuto, Suguru, Tax, Frans E., Yoshida, Shigeo, ...

Our previous studies on the endogenous brassinosteroids (BRs) in Arabidopsis have provided suggestive evidence for the operation of the early C6-oxidation and the late C6-oxidation pathways, leading...

Arabidopsis det2 Is Defective in the Conversion of (24R)-24-Methylcholest-4-En-3-One to (24R)-24-Methyl-5α-Cholestan-3-One in Brassinosteroid Biosynthesis1

Noguchi, Takahiro, Fujioka, Shozo, Takatsuto, Suguru, Sakurai, Akira, Yoshida, Shigeo, Li, Jianming, ...

Previously, we have shown that the Arabidopsis det2 (deetiolated2) mutant is defective in the biosynthesis of brassinosteroids (BR) and that DET2 (a steroid 5α-reductase) acts early in the proposed...

Brassinosteroid-Insensitive Dwarf Mutants of Arabidopsis Accumulate Brassinosteroids1

Noguchi, Takahiro, Fujioka, Shozo, Choe, Sunghwa, Takatsuto, Suguru, Yoshida, Shigeo, Yuan, Heng, ...

Seven dwarf mutants resembling brassinosteroid (BR)-biosynthetic dwarfs were isolated that did not respond significantly to the application of exogenous BRs. Genetic and molecular analyses revealed...

Brassinosteroid Levels Increase Drastically Prior to Morphogenesis of Tracheary Elements

Yamamoto, Ryo, Fujioka, Shozo, Demura, Taku, Takatsuto, Suguru, Yoshida, Shigeo, Fukuda, Hiroo

As the first step toward understanding the involvement of endogenous brassinosteroids (BRs) in cytodifferentiation, we analyzed biosynthetic activities of BRs in zinnia (Zinnia elegans L. cv Canary...

Brassinosteroid-6-Oxidases from Arabidopsis and Tomato Catalyze Multiple C-6 Oxidations in Brassinosteroid Biosynthesis1

Shimada, Yukihisa, Fujioka, Shozo, Miyauchi, Narumasa, Kushiro, Masayo, Takatsuto, Suguru, Nomura, Takahito, ...

Brassinosteroids (BRs) are steroidal plant hormones that are essential for growth and development. It has been proposed that BRs are synthesized via two parallel pathways, the early and late C-6...

Loss of Function of a Rice brassinosteroid insensitive1 Homolog Prevents Internode Elongation and Bending of the Lamina Joint

Yamamuro, Chizuko, Ihara, Yoshihisa, Wu, Xiong, Noguchi, Takahiro, Fujioka, Shozo, Takatsuto, Suguru, ...

Brassinosteroids (BRs) are plant growth–promoting natural products required for plant growth and development. Physiological studies have demonstrated that exogenous BR, alone or in combination with...

The Identification of CVP1 Reveals a Role for Sterols in Vascular Patterning

Carland, Francine M., Fujioka, Shozo, Takatsuto, Suguru, Yoshida, Shigeo, Nelson, Timothy

Vascular cell axialization refers to the uniform alignment of vascular strands. In the Arabidopsis cotyledon vascular pattern1 (cvp1) mutant, vascular cells are not arranged in parallel files and are...

An Early C-22 Oxidation Branch in the Brassinosteroid Biosynthetic Pathway

Fujioka, Shozo, Takatsuto, Suguru, Yoshida, Shigeo

The natural occurrence of 22-hydroxylated steroids in cultured Catharanthus roseus cells and in Arabidopsis seedlings was investigated. Using full-scan gas chromatography-mass spectrometry analysis,...

Arabidopsis Brassinosteroid-Insensitive dwarf12 Mutants Are Semidominant and Defective in a Glycogen Synthase Kinase 3β-Like Kinase1

Choe, Sunghwa, Schmitz, Robert J., Fujioka, Shozo, Takatsuto, Suguru, Lee, Mi-Ok, Yoshida, Shigeo, ...

Mutants defective in the biosynthesis or signaling of brassinosteroids (BRs), plant steroid hormones, display dwarfism. Loss-of-function mutants for the gene encoding the plasma membrane-located BR...

Organ-Specific Expression of Brassinosteroid-Biosynthetic Genes and Distribution of Endogenous Brassinosteroids in Arabidopsis

Shimada, Yukihisa, Goda, Hideki, Nakamura, Ayako, Takatsuto, Suguru, Fujioka, Shozo, Yoshida, Shigeo

Brassinosteroids (BRs) are steroidal plant hormones that are essential for growth and development. There is only limited information on where BRs are synthesized and used. We studied the organ...

Sterols Regulate Development and Gene Expression in Arabidopsis1

He, Jun-Xian, Fujioka, Shozo, Li, Tsai-Chi, Kang, Shin Gene, Seto, Hideharu, Takatsuto, Suguru, ...

Sterols are important not only for structural components of eukaryotic cell membranes but also for biosynthetic precursors of steroid hormones. In plants, the diverse functions of sterol-derived...

A Semidwarf Phenotype of Barley uzu Results from a Nucleotide Substitution in the Gene Encoding a Putative Brassinosteroid Receptor

Chono, Makiko, Honda, Ichiro, Zeniya, Haruko, Yoneyama, Koichi, Saisho, Daisuke, Takeda, Kazuyoshi, ...

Brassinosteroids (BRs) play important roles throughout plant growth and development. Despite the importance of clarifying the mechanism of BR-related growth regulation in cereal crops, BR-related...

A Rice Brassinosteroid-Deficient Mutant, ebisu dwarf (d2), Is Caused by a Loss of Function of a New Member of Cytochrome P450

Hong, Zhi, Ueguchi-Tanaka, Miyako, Umemura, Kazuto, Uozu, Sakurako, Fujioka, Shozo, Takatsuto, Suguru, ...

We characterized a rice dwarf mutant, ebisu dwarf (d2). It showed the pleiotropic abnormal phenotype similar to that of the rice brassinosteroid (BR)-insensitive mutant, d61. The dwarf phenotype of...

CYP72B1 Inactivates Brassinosteroid Hormones: An Intersection between Photomorphogenesis and Plant Steroid Signal Transduction1

Turk, Edward M., Fujioka, Shozo, Seto, Hideharu, Shimada, Yukihisa, Takatsuto, Suguru, Yoshida, Shigeo, ...

Active brassinosteroids, such as brassinolide (BL) and castasterone, are growth promoting plant hormones. An Arabidopsis cytochrome P450 monooxygenase encoded by CYP72B1 has been implicated in...

A critical role of sterols in embryonic patterning and meristem programming revealed by the fackel mutants of Arabidopsis thaliana

Jang, Jyan-Chyun, Fujioka, Shozo, Tasaka, Masao, Seto, Hideharu, Takatsuto, Suguru, Ishii, Akira, ...

Here we report a novel Arabidopsis dwarf mutant, fackel-J79, whose adult morphology resembles that of brassinosteroid-deficient mutants but also displays distorted embryos, supernumerary cotyledons,...

Synthesis and determination of configuration of natural 25-hydroxyvitamin D3 26,23-lactone*

Eguchi, Tadashi, Takatsuto, Suguru, Ishiguro, Masaji, Ikekawa, Nobuo, Tanaka, Yoko, DeLuca, Hector F.

The four stereoisomers of 25-hydroxyvitamin D3 26,23-lactone were synthesized by a stereoselective lactonization method. Natural 25-hydroxyvitamin D3 26,23-lactone was produced from...

Novel Biosynthetic Pathway of Castasterone from Cholesterol in Tomato1

Kim, Tae-Wuk, Chang, Soo Chul, Lee, June Seung, Takatsuto, Suguru, Yokota, Takao, Kim, Seong-Ki

Endogenous brassinosteroids (BRs) in tomato (Lycopersicon esculentum) seedlings are known to be composed of C27- and C28-BRs. The biosynthetic pathways of C27-BRs were examined using a cell-free...

Brassinosteroid Deficiency Due to Truncated Steroid 5α-Reductase Causes Dwarfism in the lk Mutant of Pea1

Nomura, Takahito, Jager, Corinne E., Kitasaka, Yukiko, Takeuchi, Keiichi, Fukami, Motohiro, Yoneyama, Koichi, ...

The endogenous brassinosteroids in the dwarf mutant lk of pea (Pisum sativum) were quantified by gas chromatography-selected ion monitoring. The levels of castasterone, 6-deoxocastasterone, and...

A Novel Cytochrome P450 Is Implicated in Brassinosteroid Biosynthesis via the Characterization of a Rice Dwarf Mutant, dwarf11, with Reduced Seed Length

Tanabe, Sumiyo, Ashikari, Motoyuki, Fujioka, Shozo, Takatsuto, Suguru, Yoshida, Shigeo, Yano, Masahiro, ...

We have characterized a rice (Oryza sativa) dwarf mutant, dwarf11 (d11), that bears seeds of reduced length. To understand the mechanism by which seed length is regulated, the D11 gene was isolated...

Triadimefon, a fungicidal triazole-type P450 inhibitor, induces brassinosteroid deficiency-like phenotypes in plants and binds to DWF4 protein in the brassinosteroid biosynthesis pathway.

Asami, Tadao, Mizutani, Masaharu, Shimada, Yukihisa, Goda, Hideki, Kitahata, Nobutaka, Sekimata, Katsuhiko, ...

Triadimefon (Bayleton), a widely used triazole-type fungicide, affects gibberellin (GA) biosynthesis and 14 alpha-demethylase in sterol biosynthesis. The present study revealed that the phenotype of...

The UGT73C5 of Arabidopsis thaliana glucosylates brassinosteroids

Poppenberger, Brigitte, Fujioka, Shozo, Soeno, Kazuo, George, Gilu L., Vaistij, Fabián E., Hiranuma, Sayoko, ...

Steroid hormones are essential for development, and the precise control of their homeostasis is a prerequisite for normal growth. UDP-glycosyltransferases (UGTs) are considered to play an important...

The Rice brassinosteroid-deficient dwarf2 Mutant, Defective in the Rice Homolog of Arabidopsis DIMINUTO/DWARF1, Is Rescued by the Endogenously Accumulated Alternative Bioactive Brassinosteroid, Dolichosterone

Hong, Zhi, Ueguchi-Tanaka, Miyako, Fujioka, Shozo, Takatsuto, Suguru, Yoshida, Shigeo, Hasegawa, Yasuko, ...

We have identified a rice (Oryza sativa) brassinosteroid (BR)-deficient mutant, BR-deficient dwarf2 (brd2). The brd2 locus contains a single base deletion in the coding region of Dim/dwf1, a homolog...

Arabidopsis CYP85A2, a Cytochrome P450, Mediates the Baeyer-Villiger Oxidation of Castasterone to Brassinolide in Brassinosteroid Biosynthesis

Kim, Tae-Wuk, Hwang, Jung-Yun, Kim, Young-Soo, Joo, Se-Hwan, Chang, Soo Chul, Lee, June Seung, ...

The conversion of castasterone (CS) to brassinolide (BL), a Baeyer-Villiger oxidation, represents the final and rate-limiting step in the biosynthesis of BL in plants. Heterologously expressed...

The tomato DWARF enzyme catalyses C-6 oxidation in brassinosteroid biosynthesis

Bishop, Gerard J., Nomura, Takahito, Yokota, Takao, Harrison, Kate, Noguchi, Takahiro, Fujioka, Shozo, ...

Brassinosteroids (BRs) are steroidal plant hormones essential for normal plant growth and development. Mutants in the biosynthesis or perception of BRs are usually dwarf. The tomato Dwarf gene (D),...

BAS1: A gene regulating brassinosteroid levels and light responsiveness in Arabidopsis

Neff, Michael M., Nguyen, Serena M., Malancharuvil, Elizabeth J., Fujioka, Shozo, Noguchi, Takahiro, Seto, Hideharu, ...

The Arabidopsis bas1-D mutation suppresses the long hypocotyl phenotype caused by mutations in the photoreceptor phytochrome B (phyB). The adult phenotype of bas1-D phyB-4 double mutants mimics that...

Brassinosteroid/Sterol Synthesis and Plant Growth as Affected by lka and lkb Mutations of Pea1

Nomura, Takahito, Kitasaka, Yukiko, Takatsuto, Suguru, Reid, James B., Fukami, Motohiro, Yokota, Takao

The dwarf pea (Pisum sativum) mutants lka and lkb are brassinosteroid (BR) insensitive and deficient, respectively. The dwarf phenotype of the lkb mutant was rescued to wild type by exogenous...

The Arabidopsis dwarf1 Mutant Is Defective in the Conversion of 24-Methylenecholesterol to Campesterol in Brassinosteroid Biosynthesis1

Choe, Sunghwa, Dilkes, Brian P., Gregory, Brian D., Ross, Amanda S., Yuan, Heng, Noguchi, Takahiro, ...

Since the isolation and characterization of dwarf1-1 (dwf1-1) from a T-DNA insertion mutant population, phenotypically similar mutants, including deetiolated2 (det2), constitutive photomorphogenesis...

A Putative Role for the Tomato Genes DUMPY and CURL-3 in Brassinosteroid Biosynthesis and Response1

Koka, Chala V., Cerny, R. Eric, Gardner, Randy G., Noguchi, Takahiro, Fujioka, Shozo, Takatsuto, Suguru, ...

The dumpy (dpy) mutant of tomato (Lycopersicon esculentum Mill.) exhibits short stature, reduced axillary branching, and altered leaf morphology. Application of brassinolide and castasterone rescued...

Characterization of Brassinazole, a Triazole-Type Brassinosteroid Biosynthesis Inhibitor1

Asami, Tadao, Min, Yong Ki, Nagata, Noriko, Yamagishi, Kazutoshi, Takatsuto, Suguru, Fujioka, Shozo, ...

Screening for brassinosteroid (BR) biosynthesis inhibitors was performed to find chemicals that induce dwarfism in Arabidopsis, mutants that resembled BR biosynthesis mutants that can be rescued by...

Biosynthetic Pathways of Brassinolide in Arabidopsis1

Noguchi, Takahiro, Fujioka, Shozo, Choe, Sunghwa, Takatsuto, Suguru, Tax, Frans E., Yoshida, Shigeo, ...

Our previous studies on the endogenous brassinosteroids (BRs) in Arabidopsis have provided suggestive evidence for the operation of the early C6-oxidation and the late C6-oxidation pathways, leading...

Arabidopsis det2 Is Defective in the Conversion of (24R)-24-Methylcholest-4-En-3-One to (24R)-24-Methyl-5α-Cholestan-3-One in Brassinosteroid Biosynthesis1

Noguchi, Takahiro, Fujioka, Shozo, Takatsuto, Suguru, Sakurai, Akira, Yoshida, Shigeo, Li, Jianming, ...

Previously, we have shown that the Arabidopsis det2 (deetiolated2) mutant is defective in the biosynthesis of brassinosteroids (BR) and that DET2 (a steroid 5α-reductase) acts early in the proposed...

Brassinosteroid-Insensitive Dwarf Mutants of Arabidopsis Accumulate Brassinosteroids1

Noguchi, Takahiro, Fujioka, Shozo, Choe, Sunghwa, Takatsuto, Suguru, Yoshida, Shigeo, Yuan, Heng, ...

Seven dwarf mutants resembling brassinosteroid (BR)-biosynthetic dwarfs were isolated that did not respond significantly to the application of exogenous BRs. Genetic and molecular analyses revealed...

Brassinosteroid Levels Increase Drastically Prior to Morphogenesis of Tracheary Elements

Yamamoto, Ryo, Fujioka, Shozo, Demura, Taku, Takatsuto, Suguru, Yoshida, Shigeo, Fukuda, Hiroo

As the first step toward understanding the involvement of endogenous brassinosteroids (BRs) in cytodifferentiation, we analyzed biosynthetic activities of BRs in zinnia (Zinnia elegans L. cv Canary...

Brassinosteroid-6-Oxidases from Arabidopsis and Tomato Catalyze Multiple C-6 Oxidations in Brassinosteroid Biosynthesis1

Shimada, Yukihisa, Fujioka, Shozo, Miyauchi, Narumasa, Kushiro, Masayo, Takatsuto, Suguru, Nomura, Takahito, ...

Brassinosteroids (BRs) are steroidal plant hormones that are essential for growth and development. It has been proposed that BRs are synthesized via two parallel pathways, the early and late C-6...

Loss of Function of a Rice brassinosteroid insensitive1 Homolog Prevents Internode Elongation and Bending of the Lamina Joint

Yamamuro, Chizuko, Ihara, Yoshihisa, Wu, Xiong, Noguchi, Takahiro, Fujioka, Shozo, Takatsuto, Suguru, ...

Brassinosteroids (BRs) are plant growth–promoting natural products required for plant growth and development. Physiological studies have demonstrated that exogenous BR, alone or in combination with...

The Identification of CVP1 Reveals a Role for Sterols in Vascular Patterning

Carland, Francine M., Fujioka, Shozo, Takatsuto, Suguru, Yoshida, Shigeo, Nelson, Timothy

Vascular cell axialization refers to the uniform alignment of vascular strands. In the Arabidopsis cotyledon vascular pattern1 (cvp1) mutant, vascular cells are not arranged in parallel files and are...

An Early C-22 Oxidation Branch in the Brassinosteroid Biosynthetic Pathway

Fujioka, Shozo, Takatsuto, Suguru, Yoshida, Shigeo

The natural occurrence of 22-hydroxylated steroids in cultured Catharanthus roseus cells and in Arabidopsis seedlings was investigated. Using full-scan gas chromatography-mass spectrometry analysis,...

Arabidopsis Brassinosteroid-Insensitive dwarf12 Mutants Are Semidominant and Defective in a Glycogen Synthase Kinase 3β-Like Kinase1

Choe, Sunghwa, Schmitz, Robert J., Fujioka, Shozo, Takatsuto, Suguru, Lee, Mi-Ok, Yoshida, Shigeo, ...

Mutants defective in the biosynthesis or signaling of brassinosteroids (BRs), plant steroid hormones, display dwarfism. Loss-of-function mutants for the gene encoding the plasma membrane-located BR...

Organ-Specific Expression of Brassinosteroid-Biosynthetic Genes and Distribution of Endogenous Brassinosteroids in Arabidopsis

Shimada, Yukihisa, Goda, Hideki, Nakamura, Ayako, Takatsuto, Suguru, Fujioka, Shozo, Yoshida, Shigeo

Brassinosteroids (BRs) are steroidal plant hormones that are essential for growth and development. There is only limited information on where BRs are synthesized and used. We studied the organ...

Sterols Regulate Development and Gene Expression in Arabidopsis1

He, Jun-Xian, Fujioka, Shozo, Li, Tsai-Chi, Kang, Shin Gene, Seto, Hideharu, Takatsuto, Suguru, ...

Sterols are important not only for structural components of eukaryotic cell membranes but also for biosynthetic precursors of steroid hormones. In plants, the diverse functions of sterol-derived...

A Semidwarf Phenotype of Barley uzu Results from a Nucleotide Substitution in the Gene Encoding a Putative Brassinosteroid Receptor

Chono, Makiko, Honda, Ichiro, Zeniya, Haruko, Yoneyama, Koichi, Saisho, Daisuke, Takeda, Kazuyoshi, ...

Brassinosteroids (BRs) play important roles throughout plant growth and development. Despite the importance of clarifying the mechanism of BR-related growth regulation in cereal crops, BR-related...

A Rice Brassinosteroid-Deficient Mutant, ebisu dwarf (d2), Is Caused by a Loss of Function of a New Member of Cytochrome P450

Hong, Zhi, Ueguchi-Tanaka, Miyako, Umemura, Kazuto, Uozu, Sakurako, Fujioka, Shozo, Takatsuto, Suguru, ...

We characterized a rice dwarf mutant, ebisu dwarf (d2). It showed the pleiotropic abnormal phenotype similar to that of the rice brassinosteroid (BR)-insensitive mutant, d61. The dwarf phenotype of...

CYP72B1 Inactivates Brassinosteroid Hormones: An Intersection between Photomorphogenesis and Plant Steroid Signal Transduction1

Turk, Edward M., Fujioka, Shozo, Seto, Hideharu, Shimada, Yukihisa, Takatsuto, Suguru, Yoshida, Shigeo, ...

Active brassinosteroids, such as brassinolide (BL) and castasterone, are growth promoting plant hormones. An Arabidopsis cytochrome P450 monooxygenase encoded by CYP72B1 has been implicated in...

A critical role of sterols in embryonic patterning and meristem programming revealed by the fackel mutants of Arabidopsis thaliana

Jang, Jyan-Chyun, Fujioka, Shozo, Tasaka, Masao, Seto, Hideharu, Takatsuto, Suguru, Ishii, Akira, ...

Here we report a novel Arabidopsis dwarf mutant, fackel-J79, whose adult morphology resembles that of brassinosteroid-deficient mutants but also displays distorted embryos, supernumerary cotyledons,...

Synthesis and determination of configuration of natural 25-hydroxyvitamin D3 26,23-lactone*

Eguchi, Tadashi, Takatsuto, Suguru, Ishiguro, Masaji, Ikekawa, Nobuo, Tanaka, Yoko, DeLuca, Hector F.

The four stereoisomers of 25-hydroxyvitamin D3 26,23-lactone were synthesized by a stereoselective lactonization method. Natural 25-hydroxyvitamin D3 26,23-lactone was produced from...

Novel Biosynthetic Pathway of Castasterone from Cholesterol in Tomato1

Kim, Tae-Wuk, Chang, Soo Chul, Lee, June Seung, Takatsuto, Suguru, Yokota, Takao, Kim, Seong-Ki

Endogenous brassinosteroids (BRs) in tomato (Lycopersicon esculentum) seedlings are known to be composed of C27- and C28-BRs. The biosynthetic pathways of C27-BRs were examined using a cell-free...

Brassinosteroid Deficiency Due to Truncated Steroid 5α-Reductase Causes Dwarfism in the lk Mutant of Pea1

Nomura, Takahito, Jager, Corinne E., Kitasaka, Yukiko, Takeuchi, Keiichi, Fukami, Motohiro, Yoneyama, Koichi, ...

The endogenous brassinosteroids in the dwarf mutant lk of pea (Pisum sativum) were quantified by gas chromatography-selected ion monitoring. The levels of castasterone, 6-deoxocastasterone, and...

A Novel Cytochrome P450 Is Implicated in Brassinosteroid Biosynthesis via the Characterization of a Rice Dwarf Mutant, dwarf11, with Reduced Seed Length

Tanabe, Sumiyo, Ashikari, Motoyuki, Fujioka, Shozo, Takatsuto, Suguru, Yoshida, Shigeo, Yano, Masahiro, ...

We have characterized a rice (Oryza sativa) dwarf mutant, dwarf11 (d11), that bears seeds of reduced length. To understand the mechanism by which seed length is regulated, the D11 gene was isolated...

The Rice brassinosteroid-deficient dwarf2 Mutant, Defective in the Rice Homolog of Arabidopsis DIMINUTO/DWARF1, Is Rescued by the Endogenously Accumulated Alternative Bioactive Brassinosteroid, Dolichosterone

Hong, Zhi, Ueguchi-Tanaka, Miyako, Fujioka, Shozo, Takatsuto, Suguru, Yoshida, Shigeo, Hasegawa, Yasuko, ...

We have identified a rice (Oryza sativa) brassinosteroid (BR)-deficient mutant, BR-deficient dwarf2 (brd2). The brd2 locus contains a single base deletion in the coding region of Dim/dwf1, a homolog...

Arabidopsis CYP85A2, a Cytochrome P450, Mediates the Baeyer-Villiger Oxidation of Castasterone to Brassinolide in Brassinosteroid Biosynthesis

Kim, Tae-Wuk, Hwang, Jung-Yun, Kim, Young-Soo, Joo, Se-Hwan, Chang, Soo Chul, Lee, June Seung, ...

The conversion of castasterone (CS) to brassinolide (BL), a Baeyer-Villiger oxidation, represents the final and rate-limiting step in the biosynthesis of BL in plants. Heterologously expressed...

Triadimefon, a fungicidal triazole-type P450 inhibitor, induces brassinosteroid deficiency-like phenotypes in plants and binds to DWF4 protein in the brassinosteroid biosynthesis pathway.

Asami, Tadao, Mizutani, Masaharu, Shimada, Yukihisa, Goda, Hideki, Kitahata, Nobutaka, Sekimata, Katsuhiko, ...

Triadimefon (Bayleton), a widely used triazole-type fungicide, affects gibberellin (GA) biosynthesis and 14 alpha-demethylase in sterol biosynthesis. The present study revealed that the phenotype of...

The UGT73C5 of Arabidopsis thaliana glucosylates brassinosteroids

Poppenberger, Brigitte, Fujioka, Shozo, Soeno, Kazuo, George, Gilu L., Vaistij, Fabián E., Hiranuma, Sayoko, ...

Steroid hormones are essential for development, and the precise control of their homeostasis is a prerequisite for normal growth. UDP-glycosyltransferases (UGTs) are considered to play an important...

The Regulation of DWARF4 Expression Is Likely a Critical Mechanism in Maintaining the Homeostasis of Bioactive Brassinosteroids in Arabidopsis1

Kim, Ho Bang, Kwon, Mi, Ryu, Hojin, Fujioka, Shozo, Takatsuto, Suguru, Yoshida, Shigeo, ...

Mutants that are defective in brassinosteroid (BR) biosynthesis or signaling display severely retarded growth patterns due to absence of growth-promoting effects by BRs. Arabidopsis (Arabidopsis...

The Role of OsBRI1 and Its Homologous Genes, OsBRL1 and OsBRL3, in Rice1

Nakamura, Ayako, Fujioka, Shozo, Sunohara, Hidehiko, Kamiya, Noriko, Hong, Zhi, Inukai, Yoshiaki, ...

Since first identifying two alleles of a rice (Oryza sativa) brassinosteroid (BR)-insensitive mutant, d61, that were also defective in an orthologous gene in Arabidopsis (Arabidopsis thaliana)...

Roles of Brassinosteroids and Related mRNAs in Pea Seed Growth and Germination1[W][OA]

Nomura, Takahito, Ueno, Masaaki, Yamada, Yumiko, Takatsuto, Suguru, Takeuchi, Yasutomo, Yokota, Takao

The levels of endogenous brassinosteroids (BRs) and the expression of the biosynthesis/metabolism/perception genes involved have been investigated during the development and germination of pea (Pisum...

Brassinosteroids Regulate Grain Filling in Rice[W][OA]

Wu, Chuan-yin, Trieu, Anthony, Radhakrishnan, Parthiban, Kwok, Shing F., Harris, Sam, Zhang, Ke, ...

Genes controlling hormone levels have been used to increase grain yields in wheat (Triticum aestivum) and rice (Oryza sativa). We created transgenic rice plants expressing maize (Zea mays), rice, or...