Sumiko Araki

Removal of histone tails from nucleosome dissects the physical mechanisms of salt-induced aggregation, linker histone H1-induced compaction, and 30-nm fiber formation of the nucleosome array (2009)

Hizume, Kohji, Nakai, Tonau, Araki, Sumiko, Prieto, Eloise, Yoshikawa, Kenichi, Takeyasu, Kunio

In order to reveal the roles of histone tails in the formation of higher-order chromatin structures, we employed atomic force microscopy (AFM), and an in vitro reconstitution system to examine the...

Topoisomerase II, scaffold component, promotes chromatin compaction in vitro in a linker-histone H1-dependent manner (2007)

Hizume, Kohji, Araki, Sumiko, Yoshikawa, Kenichi, Takeyasu, Kunio

TopoisomeraseII (Topo II) is a major component of chromosomal scaffolds and essential for mitotic chromosome condensation, but the mechanism of this action remains unknown. Here, we used an in vitro...

Topoisomerase II, scaffold component, promotes chromatin compaction in vitro in a linker-histone H1-dependent manner (2007)

Hizume, Kohji, Araki, Sumiko, Yosihkawa, Kenichi, Takeyasu, Kunio

TopoisomeraseII (Topo II) is a major component of chromosomal scaffolds and essential for mitotic chromosome condensation, but the mechanism of this action remains unknown. Here, we used an in vitro...

Protective effect of vitamin C against double-strand breaks in reconstituted chromatin visualized by single-molecule observation (2006)

Yoshikawa, Yuko, Hizume, Kohji, Oda, Yoshiko, Takeyasu, Kunio, Araki, Sumiko, Yoshikawa, Kenichi

Direct attack to genomic DNA by reactive oxygen species causes various types of lesions, including base modi. cations and strand breaks. The most significant lesion is considered to be an unrepaired...

Hydrodynamic radius of circular DNA is larger than that of linear DNA (2006)

Araki, Sumiko, Nakai, Tonau, Hizume, Kohji, Takeyasu, Kunio, Yoshikawa, Kenichi

It is generally accepted that linear polymers are larger, such as with regard to the end-to-end distance and gyration radius, than the corresponding circular polymers. We measured the Brownian motion...

Protective Effect of Vitamin C against Double-Strand Breaks in Reconstituted Chromatin Visualized by Single-Molecule Observation

Yoshikawa, Yuko, Hizume, Kohji, Oda, Yoshiko, Takeyasu, Kunio, Araki, Sumiko, Yoshikawa, Kenichi

Direct attack to genomic DNA by reactive oxygen species causes various types of lesions, including base modifications and strand breaks. The most significant lesion is considered to be an unrepaired...

Topoisomerase II, scaffold component, promotes chromatin compaction in vitro in a linker-histone H1-dependent manner

Hizume, Kohji, Araki, Sumiko, Yoshikawa, Kenichi, Takeyasu, Kunio

TopoisomeraseII (Topo II) is a major component of chromosomal scaffolds and essential for mitotic chromosome condensation, but the mechanism of this action remains unknown. Here, we used an in vitro...

ATP-Induced Shrinkage of DNA with MukB Protein and the MukBEF Complex of Escherichia coli▿

Chen, Ning, Zinchenko, Anatoly A., Yoshikawa, Yuko, Araki, Sumiko, Adachi, Shun, Yamazoe, Mitsuyoshi, ...

Fluorescence microscopic observation of individual T4 DNA molecules revealed that the MukBEF complex (bacterial condensin) and its subunit, the MukB (a member of the SMC [structural maintenance of...