Improving Gene-finding in Chlamydomonas reinhardtii:GreenGenie2 (2009)
Kwan, Alan L, Li, Linya, Kulp, David C, Dutcher, Susan K, Stormo, Gary D
Abstract Background The availability of whole-genome sequences allows for the identification of the entire set of protein coding genes as well as their regulatory regions. This can be accomplished...
Procom: a web-based tool to compare multiple eukaryotic proteomes (2005)
Li, Jin Billy, Zhang, Miao, Dutcher, Susan K., Stormo, Gary D.
Summary: Each organism has traits that are shared with some, but not all, organisms. Identification of genes needed for a particular trait can be accomplished by a comparative genomics approach using...
Procom: a web-based tool to compare multiple eukaryotic proteomes (2004)
Li, Jin Billy, Zhang, Miao, Dutcher, Susan K., Stormo, Gary D.
Summary: Each organism has traits that are shared with some, but not all organisms. Identification of genes needed for a particular trait can be accomplished by a comparative genomics approach using...
Procom: a web-based tool to compare multiple eukaryotic proteomes (2004)
Li, Jin Billy, Zhang, Miao, Dutcher, Susan K., Stormo, Gary D.
Summary: Each organism has traits that are shared with some, but not all organisms. Identification of genes needed for a particular trait can be accomplished by a comparative genomics approach using...
Procom: a web-based tool to compare multiple eukaryotic proteomes (2004)
Li, Jin Billy, Zhang, Miao, Dutcher, Susan K., Stormo, Gary D.
Summary: Each organism has traits that are shared with some, but not all organisms. Identification of genes needed for a particular trait can be accomplished by a comparative genomics approach using...
Procom: a web-based tool to compare multiple eukaryotic proteomes (2004)
Li, Jin Billy, Zhang, Miao, Dutcher, Susan K., Stormo, Gary D.
Summary: Each organism has traits that are shared with some, but not all organisms. Identification of genes needed for a particular trait can be accomplished by a comparative genomics approach using...
JIN BILLY LI, SHAOPING LIN, HONGGUI JIA, HONGMIN WU, BRUCE A. ROE, DAVID KULP, ...
Chlamydomonas reinhardtii is a unicellular green alga that has been used as a model organism for the study of flagella and basal bodies as well as photosynthesis. This report analyzes finished...
Chlamydomonas reinhardtii: Biological Rationale for Genomics1 (2000)
Chlamydomonas reinhardtii has been the subject of genetic, biochemical, cytological, and molecular analyses for over 50 years. It is an ideal model system for the study of flagella and basal bodies...
Dutcher, Susan K., Trabuco, Emanuel C.
We have cloned the UNI3 gene in Chlamydomonas and find that it encodes a new member of the tubulin superfamily. Although Uni3p shares significant sequence identity with α-, β-, and γ-tubulins,...
Palombella, Anthony L., Dutcher, Susan K.
We report the isolation of a Chlamydomonas reinhardtii cDNA that encodes the β-subunit of tryptophan synthase (TSB). This cDNA was cloned by functional complementation of a trp-operon-deleted strain...
ε-Tubulin Is an Essential Component of the Centriole
Dutcher, Susan K., Morrissette, Naomi S., Preble, Andrea M., Rackley, Craig, Stanga, John
Centrioles and basal bodies are cylinders composed of nine triplet microtubule blades that play essential roles in the centrosome and in flagellar assembly. Chlamydomonas cells with the bld2-1...
O'Toole, Eileen T., Giddings, Thomas H., McIntosh, J. Richard, Dutcher, Susan K.
Improved methods of specimen preparation and dual-axis electron tomography have been used to study the structure and organization of basal bodies in the unicellular alga Chlamydomonas reinhardtii....
Mutant Kinesin-2 Motor Subunits Increase Chromosome Loss
Miller, Mark S., Esparza, Jessica M., Lippa, Andrew M., Lux, Fordyce G., Cole, Douglas G., Dutcher, Susan K.
The Chlamydomonas anterograde intraflagellar transport motor, kinesin-2, is isolated as a heterotrimeric complex containing two motor subunits and a nonmotor subunit known as kinesin-associated...
The Role of S. CEREVISIAE Cell Division Cycle Genes in Nuclear Fusion
Dutcher, Susan K., Hartwell, Leland H.
Forty temperature-sensitive cell division cycle (cdc) mutants of Saccharomyces cerevisiae were examined for their ability to complete nuclear fusion during conjugation in crosses to a CDC parent...
Mutations in CDC genes of S. cerevisiae disrupt the cell cycle at specific stages. The experiments reported here demonstrate that two CDC genes, CDC5 and CDC27, are necessary for mitochondrial...
Bowers, Amber K, Keller, Jennifer A, Dutcher, Susan K
To take advantage of available expressed sequence tags and genomic sequence, we have developed 64 PCR-based molecular markers in Chlamydomonas reinhardtii that map to the 17 linkage groups. These...
Dutcher, Susan K., Trabuco, Emanuel C.
We have cloned the UNI3 gene in Chlamydomonas and find that it encodes a new member of the tubulin superfamily. Although Uni3p shares significant sequence identity with α-, β-, and γ-tubulins,...
Palombella, Anthony L., Dutcher, Susan K.
We report the isolation of a Chlamydomonas reinhardtii cDNA that encodes the β-subunit of tryptophan synthase (TSB). This cDNA was cloned by functional complementation of a trp-operon-deleted strain...
ε-Tubulin Is an Essential Component of the Centriole
Dutcher, Susan K., Morrissette, Naomi S., Preble, Andrea M., Rackley, Craig, Stanga, John
Centrioles and basal bodies are cylinders composed of nine triplet microtubule blades that play essential roles in the centrosome and in flagellar assembly. Chlamydomonas cells with the bld2-1...
O'Toole, Eileen T., Giddings, Thomas H., McIntosh, J. Richard, Dutcher, Susan K.
Improved methods of specimen preparation and dual-axis electron tomography have been used to study the structure and organization of basal bodies in the unicellular alga Chlamydomonas reinhardtii....
Mutant Kinesin-2 Motor Subunits Increase Chromosome Loss
Miller, Mark S., Esparza, Jessica M., Lippa, Andrew M., Lux, Fordyce G., Cole, Douglas G., Dutcher, Susan K.
The Chlamydomonas anterograde intraflagellar transport motor, kinesin-2, is isolated as a heterotrimeric complex containing two motor subunits and a nonmotor subunit known as kinesin-associated...
The Role of S. CEREVISIAE Cell Division Cycle Genes in Nuclear Fusion
Dutcher, Susan K., Hartwell, Leland H.
Forty temperature-sensitive cell division cycle (cdc) mutants of Saccharomyces cerevisiae were examined for their ability to complete nuclear fusion during conjugation in crosses to a CDC parent...
Mutations in CDC genes of S. cerevisiae disrupt the cell cycle at specific stages. The experiments reported here demonstrate that two CDC genes, CDC5 and CDC27, are necessary for mitochondrial...
Bowers, Amber K, Keller, Jennifer A, Dutcher, Susan K
To take advantage of available expressed sequence tags and genomic sequence, we have developed 64 PCR-based molecular markers in Chlamydomonas reinhardtii that map to the 17 linkage groups. These...
King, Stephen J., Dutcher, Susan K.
To gain a further understanding of axonemal dynein regulation, mutant strains of Chlamydomonas reinhardtii that had defects in both phototactic behavior and flagellar motility were identified and...