Beall, Bernard, Gherardi, Giovanni, Facklam, Richard R., Hollingshead, Susan K.
In a recent genotypic survey of β-lactam-resistant pneumococci recovered in different areas of United States during 1997, eight clonal types that each represented 3 to 40 isolates accounted for 134...
Briles, David E., Tart, Rebecca Creech, Swiatlo, Edwin, Dillard, Joseph P., Smith, Patricia, Benton, Kimberly A., ...
Streptococcus pneumoniae is a problematic infectious agent, whose seriousness to human health has been underscored by the recent rise in the frequency of isolation of multidrug-resistant strains....
The Autolytic Enzyme LytA of Streptococcus pneumoniae Is Not Responsible for Releasing Pneumolysin
Balachandran, Priya, Hollingshead, Susan K., Paton, James C., Briles, David E.
It was previously proposed that autolysin's primary role in the virulence of pneumococci was to release pneumolysin to an extracellular location. This interpretation came into question when...
Brooks-Walter, Alexis, Briles, David E., Hollingshead, Susan K.
PspC is one of three designations for a pneumococcal surface protein whose gene is present in approximately 75% of all Streptococcus pneumoniae strains. Under the name SpsA, the protein has been...
Yung, Der-Li, McIver, Kevin S., Scott, June R., Hollingshead, Susan K.
The attenuated expression of virulence genes found in a group A streptococcal strain that is naturally pathogenic for mice was postulated to result from a defect in the strain's multigene regulator,...
Briles, David E., Ades, Eddie, Paton, James C., Sampson, Jacquelyn S., Carlone, George M., Huebner, Robert C., ...
Acquisition of pneumococci is generally from carriers rather than from infected individuals. Therefore, to induce herd immunity against Streptococcus pneumoniae it will be necessary to elicit...
Ogunniyi, A. David, Folland, Rebekah L., Briles, David E., Hollingshead, Susan K., Paton, James C.
The vaccine potential of a combination of three pneumococcal virulence proteins was evaluated in an active-immunization–intraperitoneal-challenge model in BALB/c mice, using very high challenge...
Tandem Repeat Deletion in the Alpha C Protein of Group B Streptococcus Is recA Independent
Puopolo, Karen M., Hollingshead, Susan K., Carey, Vincent J., Madoff, Lawrence C.
Group B streptococci (GBS) contain a family of protective surface proteins characterized by variable numbers of repeating units within the proteins. The prototype alpha C protein of GBS from the type...
Diversity of PspA: Mosaic Genes and Evidence for Past Recombination in Streptococcus pneumoniae
Hollingshead, Susan K., Becker, Robert, Briles, David E.
Pneumococcal surface protein A (PspA) is a serologically variable protein of Streptococcus pneumoniae. Twenty-four diverse alleles of the pspA gene were sequenced to investigate the genetic basis for...
McDaniel, Larry S., McDaniel, D. Olga, Hollingshead, Susan K., Briles, David E.
PspA (pneumococcal surface protein A) is a serologically varied virulence factor of Streptococcus pneumoniae. In mice, PspA has been shown to elicit an antibody response that protects against fatal...
Balachandran, Priya, Brooks-Walter, Alexis, Virolainen-Julkunen, Anni, Hollingshead, Susan K., Briles, David E.
Previous studies suggested that PspC is important in adherence and colonization within the nasopharynx. In this study, we conducted mutational studies to further identify the role PspC plays in the...
Ren, Bing, Szalai, Alexander J., Thomas, Orlanda, Hollingshead, Susan K., Briles, David E.
Pneumococcal surface protein A (PspA), a virulence factor of Streptococcus pneumoniae, is exceptionally diverse, being classified into two major families which are over 50% divergent by sequence...
Roche, Hazeline, Håkansson, Anders, Hollingshead, Susan K., Briles, David E.
Pneumococcal surface protein A (PspA) can elicit protection against Streptococcus pneumoniae in mouse infection models. PspA is classified by serology and amino acid sequence into two major families...
Evolution and Virulence of Serogroup 6 Pneumococci on a Global Scale
Robinson, D. Ashley, Briles, David E., Crain, Marilyn J., Hollingshead, Susan K.
To study the evolution and virulence of pneumococcal populations, we used multilocus sequence typing to identify the major clones among 212 carriage and invasive isolates expressing capsular...
Strand, Tor A., Hollingshead, Susan K., Julshamn, Kåre, Briles, David E., Blomberg, Bjørn, Sommerfelt, Halvor
Streptococcus pneumoniae is a major cause of illness and death in children in developing countries. In these children, zinc deficiency is associated with an increased risk of acute respiratory tract...
Johnson, Crystal N., Moser, Stephen A., Hollingshead, Susan K., Zheng, Xiaotian, Crain, Marilyn J., ...
We characterized 32 levofloxacin-nonsusceptible Streptococcus pneumoniae (LNSP) isolates obtained from a broad geographic region of North America over a 5-year period by using capsular serotypes,...
Waites, Ken B., Jones, Kellie E., Kim, Kyung Hyo, Moser, Stephen A., Johnson, Crystal N., Hollingshead, Susan K., ...
Macrolide resistance was detected in 64 of 77 (83.1%) Streptococcus pneumoniae isolates from South Korea. Seven (10.9%) isolates contained only mef(A), 32 (50%) contained only erm(B), and 25 (39.1%)...
Ren, Bing, Szalai, Alexander J., Hollingshead, Susan K., Briles, David E.
Streptococcus pneumoniae infection is a frequent cause of pneumonia, otitis media, meningitis, and septicemia. Pneumococcal surface protein A (PspA) is an important virulence factor on the pathogen...
Shaper, Mirza, Hollingshead, Susan K., Benjamin, William H., Briles, David E.
Lactoferrin is an important component of innate immunity through its sequestration of iron, bactericidal activity, and immune modulatory activity. Apolactoferrin (ALF) is the iron-depleted form of...
Johnston, Jason W., Myers, Lisa E., Ochs, Martina M., Benjamin, William H., Briles, David E., Hollingshead, Susan K.
PsaA of Streptococcus pneumoniae, originally believed to be an adhesin, is the lipoprotein component of an Mn2+ transporter. Mutations in psaA cause deficiencies in growth, virulence, adherence, and...
Evolutionary Genetics of the Capsular Locus of Serogroup 6 Pneumococci
Mavroidi, Angeliki, Godoy, Daniel, Aanensen, David M., Robinson, D. Ashley, Hollingshead, Susan K., Spratt, Brian G.
The evolution of the capsular biosynthetic (cps) locus of serogroup 6 Streptococcus pneumoniae was investigated by analyzing sequence variation within three serotype-specific cps genes from 102...
Palaniappan, Ravichandran, Singh, Shailesh, Singh, Udai P., Sakthivel, Senthil Kumar K., Ades, Edwin W., Briles, David E., ...
Larger numbers of pneumococci were detected in the nasal tract compared to the lung, cervical lymph nodes, and spleen 1, 2, 4, 7, 14, and 21 days after nasal challenge with Streptococcus pneumoniae...
Johnston, Jason W., Briles, David E., Myers, Lisa E., Hollingshead, Susan K.
The concentration of Mn2+ is 1,000-fold higher in secretions than it is at internal sites of the body, making it a potential signal by which bacteria can sense a shift from a mucosal environment to a...
Validation of a Multiplex Pneumococcal Serotyping Assay with Clinical Samples
Lin, Jisheng, Kaltoft, Margit S., Brandao, Angela P., Echaniz-Aviles, Gabriela, Brandileone, M. Cristina C., Hollingshead, Susan K., ...
We have recently developed a rapid pneumococcal serotyping method called “multibead assay” (J. Yu et al., J. Clin. Microbiol. 43:156-162, 2005) based on a multiplexed immunoassay for capsular...
Beall, Bernard, Gherardi, Giovanni, Facklam, Richard R., Hollingshead, Susan K.
In a recent genotypic survey of β-lactam-resistant pneumococci recovered in different areas of United States during 1997, eight clonal types that each represented 3 to 40 isolates accounted for 134...
Briles, David E., Tart, Rebecca Creech, Swiatlo, Edwin, Dillard, Joseph P., Smith, Patricia, Benton, Kimberly A., ...
Streptococcus pneumoniae is a problematic infectious agent, whose seriousness to human health has been underscored by the recent rise in the frequency of isolation of multidrug-resistant strains....
The Autolytic Enzyme LytA of Streptococcus pneumoniae Is Not Responsible for Releasing Pneumolysin
Balachandran, Priya, Hollingshead, Susan K., Paton, James C., Briles, David E.
It was previously proposed that autolysin's primary role in the virulence of pneumococci was to release pneumolysin to an extracellular location. This interpretation came into question when...
Brooks-Walter, Alexis, Briles, David E., Hollingshead, Susan K.
PspC is one of three designations for a pneumococcal surface protein whose gene is present in approximately 75% of all Streptococcus pneumoniae strains. Under the name SpsA, the protein has been...
Yung, Der-Li, McIver, Kevin S., Scott, June R., Hollingshead, Susan K.
The attenuated expression of virulence genes found in a group A streptococcal strain that is naturally pathogenic for mice was postulated to result from a defect in the strain's multigene regulator,...
Briles, David E., Ades, Eddie, Paton, James C., Sampson, Jacquelyn S., Carlone, George M., Huebner, Robert C., ...
Acquisition of pneumococci is generally from carriers rather than from infected individuals. Therefore, to induce herd immunity against Streptococcus pneumoniae it will be necessary to elicit...
Ogunniyi, A. David, Folland, Rebekah L., Briles, David E., Hollingshead, Susan K., Paton, James C.
The vaccine potential of a combination of three pneumococcal virulence proteins was evaluated in an active-immunization–intraperitoneal-challenge model in BALB/c mice, using very high challenge...
Tandem Repeat Deletion in the Alpha C Protein of Group B Streptococcus Is recA Independent
Puopolo, Karen M., Hollingshead, Susan K., Carey, Vincent J., Madoff, Lawrence C.
Group B streptococci (GBS) contain a family of protective surface proteins characterized by variable numbers of repeating units within the proteins. The prototype alpha C protein of GBS from the type...
Diversity of PspA: Mosaic Genes and Evidence for Past Recombination in Streptococcus pneumoniae
Hollingshead, Susan K., Becker, Robert, Briles, David E.
Pneumococcal surface protein A (PspA) is a serologically variable protein of Streptococcus pneumoniae. Twenty-four diverse alleles of the pspA gene were sequenced to investigate the genetic basis for...
McDaniel, Larry S., McDaniel, D. Olga, Hollingshead, Susan K., Briles, David E.
PspA (pneumococcal surface protein A) is a serologically varied virulence factor of Streptococcus pneumoniae. In mice, PspA has been shown to elicit an antibody response that protects against fatal...
Balachandran, Priya, Brooks-Walter, Alexis, Virolainen-Julkunen, Anni, Hollingshead, Susan K., Briles, David E.
Previous studies suggested that PspC is important in adherence and colonization within the nasopharynx. In this study, we conducted mutational studies to further identify the role PspC plays in the...
Ren, Bing, Szalai, Alexander J., Thomas, Orlanda, Hollingshead, Susan K., Briles, David E.
Pneumococcal surface protein A (PspA), a virulence factor of Streptococcus pneumoniae, is exceptionally diverse, being classified into two major families which are over 50% divergent by sequence...
Roche, Hazeline, Håkansson, Anders, Hollingshead, Susan K., Briles, David E.
Pneumococcal surface protein A (PspA) can elicit protection against Streptococcus pneumoniae in mouse infection models. PspA is classified by serology and amino acid sequence into two major families...
Evolution and Virulence of Serogroup 6 Pneumococci on a Global Scale
Robinson, D. Ashley, Briles, David E., Crain, Marilyn J., Hollingshead, Susan K.
To study the evolution and virulence of pneumococcal populations, we used multilocus sequence typing to identify the major clones among 212 carriage and invasive isolates expressing capsular...
Strand, Tor A., Hollingshead, Susan K., Julshamn, Kåre, Briles, David E., Blomberg, Bjørn, Sommerfelt, Halvor
Streptococcus pneumoniae is a major cause of illness and death in children in developing countries. In these children, zinc deficiency is associated with an increased risk of acute respiratory tract...
Johnson, Crystal N., Moser, Stephen A., Hollingshead, Susan K., Zheng, Xiaotian, Crain, Marilyn J., ...
We characterized 32 levofloxacin-nonsusceptible Streptococcus pneumoniae (LNSP) isolates obtained from a broad geographic region of North America over a 5-year period by using capsular serotypes,...
Waites, Ken B., Jones, Kellie E., Kim, Kyung Hyo, Moser, Stephen A., Johnson, Crystal N., Hollingshead, Susan K., ...
Macrolide resistance was detected in 64 of 77 (83.1%) Streptococcus pneumoniae isolates from South Korea. Seven (10.9%) isolates contained only mef(A), 32 (50%) contained only erm(B), and 25 (39.1%)...
Ren, Bing, Szalai, Alexander J., Hollingshead, Susan K., Briles, David E.
Streptococcus pneumoniae infection is a frequent cause of pneumonia, otitis media, meningitis, and septicemia. Pneumococcal surface protein A (PspA) is an important virulence factor on the pathogen...
Shaper, Mirza, Hollingshead, Susan K., Benjamin, William H., Briles, David E.
Lactoferrin is an important component of innate immunity through its sequestration of iron, bactericidal activity, and immune modulatory activity. Apolactoferrin (ALF) is the iron-depleted form of...
Johnston, Jason W., Myers, Lisa E., Ochs, Martina M., Benjamin, William H., Briles, David E., Hollingshead, Susan K.
PsaA of Streptococcus pneumoniae, originally believed to be an adhesin, is the lipoprotein component of an Mn2+ transporter. Mutations in psaA cause deficiencies in growth, virulence, adherence, and...
Evolutionary Genetics of the Capsular Locus of Serogroup 6 Pneumococci
Mavroidi, Angeliki, Godoy, Daniel, Aanensen, David M., Robinson, D. Ashley, Hollingshead, Susan K., Spratt, Brian G.
The evolution of the capsular biosynthetic (cps) locus of serogroup 6 Streptococcus pneumoniae was investigated by analyzing sequence variation within three serotype-specific cps genes from 102...
Palaniappan, Ravichandran, Singh, Shailesh, Singh, Udai P., Sakthivel, Senthil Kumar K., Ades, Edwin W., Briles, David E., ...
Larger numbers of pneumococci were detected in the nasal tract compared to the lung, cervical lymph nodes, and spleen 1, 2, 4, 7, 14, and 21 days after nasal challenge with Streptococcus pneumoniae...
Johnston, Jason W., Briles, David E., Myers, Lisa E., Hollingshead, Susan K.
The concentration of Mn2+ is 1,000-fold higher in secretions than it is at internal sites of the body, making it a potential signal by which bacteria can sense a shift from a mucosal environment to a...
Validation of a Multiplex Pneumococcal Serotyping Assay with Clinical Samples
Lin, Jisheng, Kaltoft, Margit S., Brandao, Angela P., Echaniz-Aviles, Gabriela, Brandileone, M. Cristina C., Hollingshead, Susan K., ...
We have recently developed a rapid pneumococcal serotyping method called “multibead assay” (J. Yu et al., J. Clin. Microbiol. 43:156-162, 2005) based on a multiplexed immunoassay for capsular...
Genetic Basis for the New Pneumococcal Serotype, 6C▿
Park, In Ho, Park, Saeyoung, Hollingshead, Susan K., Nahm, Moon H.
We have recently reported a new pneumococcal serotype (6C), which is closely related to serotype 6A (I. H. Park et al., J. Clin. Microbiol. 45:1225-1233, 2007). To investigate the genetic basis for...
Basset, Alan, Thompson, Claudette M., Hollingshead, Susan K., Briles, David E., Ades, Edwin W., Lipsitch, Marc, ...
Immunity to pneumococcal colonization in mice by exposure to live or killed pneumococci has been shown to be antibody independent but dependent on CD4+ T cells. Here we show that intranasal...
Li, Jie, Glover, David T., Szalai, Alexander J., Hollingshead, Susan K., Briles, David E.
Pneumococcal surface protein A (PspA) and PspC are important virulence factors. Their absence has been shown to allow improved clearance of pneumococci from the blood of mice and to decrease...
Glover, David T., Hollingshead, Susan K., Briles, David E.
Previous studies have suggested that pneumococcal choline binding protein A (PcpA) is important for the full virulence of Streptococcus pneumoniae, and its amino acid sequence suggests that it may...