Susan L. Forsburg

Efficient labeling of fission yeast Schizosaccharomyces pombe with thymidine and BUdR (2003)

Hodson, Jeffrey A., Bailis, Julie M., Forsburg, Susan L.

In this paper we report the construction of a Schizosaccharomyces pombe strain that facilitates analysis of replicating DNA. The strain co‐expresses the Herpes simplex virus thymidine kinase...

RNAi hushes heterochromatin (2002)

Bailis, Julie M, Forsburg, Susan L

Abstract Repeated DNA elements and region-specific protein modifications combine within chromosomes to form a transcriptionally silent chromatin structure called heterochromatin. Recent work in the...

Fission yeast enters a joyful new era (2002)

Gómez, Eliana B, Bailis, Julie M, Forsburg, Susan L

A report on the Second International Fission Yeast Meeting, Kyoto, Japan, 25-30 March 2002.

Dynamic chromosomes (2000)

Pasion, Sally G, Gómez, Eliana B, Forsburg, Susan L

A report on the sixth biannual FASEB conference on Yeast Chromosome Structure, Replication and Segregation held in Snowmass, Colorado, 19-24 August, 2000.

Isolation of an essential Schizosaccharomyces pombe gene, prp31+, that links splicing and meiosis (2000)

Bishop, Danielle T., McDonald, W. Hayes, Gould, Kathleen L., Forsburg, Susan L.

We carried out a screen for mutants that arrest prior to premeiotic S phase. One of the strains we isolated contains a temperature-sensitive allele mutation in the fission yeast prp31+ gene. The...

Multiple Domains of Fission Yeast Cdc19p (MCM2) Are Required for Its Association with the Core MCM Complex

Sherman, Daniel A., Pasion, Sally G., Forsburg, Susan L.

The members of the MCM protein family are essential eukaryotic DNA replication factors that form a six-member protein complex. In this study, we use antibodies to four MCM proteins to investigate the...

Identification of a Second Myosin-II in Schizosaccharomyces pombe:: Myp2p Is Conditionally Required for Cytokinesis

Bezanilla, Magdalena, Forsburg, Susan L., Pollard, Thomas D.

As in many eukaryotic cells, fission yeast cytokinesis depends on the assembly of an actin ring. We cloned myp2+, a myosin-II in Schizosaccharomyces pombe, conditionally required for cytokinesis....

Nuclear Localization of Schizosaccharomyces pombe Mcm2/Cdc19p Requires MCM Complex Assembly

Pasion, Sally G., Forsburg, Susan L.

The minichromosome maintenance (MCM) proteins MCM2–MCM7 are conserved eukaryotic replication factors that assemble in a heterohexameric complex. In fission yeast, these proteins are nuclear...

Schizosaccharomyces pombe Hsk1p Is a Potential Cds1p Target Required for Genome Integrity

Snaith, Hilary A., Brown, Grant W., Forsburg, Susan L.

The fission yeast Hsk1p kinase is an essential activator of DNA replication. Here we report the isolation and characterization of a novel mutant allele of the gene. Consistent with its role in the...

The Schizosaccharomyces pombe Aurora–related Kinase Ark1 Interacts with the Inner Centromere Protein Pic1 and Mediates Chromosome Segregation and Cytokinesis

Leverson, Joel D., Huang, Han-kuei, Forsburg, Susan L., Hunter, Tony

The chromosomal passenger proteins aurora-B, survivin, and inner centromere protein (INCENP) have been implicated in coordinating chromosome segregation with cell division. This work describes the...

Isolation of an essential Schizosaccharomyces pombe gene, prp31+, that links splicing and meiosis

Bishop, Danielle T., McDonald, W. Hayes, Gould, Kathleen L., Forsburg, Susan L.

We carried out a screen for mutants that arrest prior to premeiotic S phase. One of the strains we isolated contains a temperature-sensitive allele mutation in the fission yeast prp31+ gene. The...

Dynamic chromosomes

Pasion, Sally G, Gómez, Eliana B, Forsburg, Susan L

A report on the sixth biannual FASEB conference on Yeast Chromosome Structure, Replication and Segregation held in Snowmass, Colorado, 19-24 August, 2000.

Fission yeast enters a joyful new era

Gómez, Eliana B, Bailis, Julie M, Forsburg, Susan L

A report on the Second International Fission Yeast Meeting, Kyoto, Japan, 25-30 March 2002.

RNAi hushes heterochromatin

Bailis, Julie M, Forsburg, Susan L

Recent work in the fission yeast Schizosaccharomyces pombe reveals that RNA is an integral component of silent heterochromatin.

Schizosaccharomyces pombe Rdh54 (TID1) Acts with Rhp54 (RAD54) to Repair Meiotic Double-Strand Breaks V⃞

Catlett, Michael G., Forsburg, Susan L.

We report the characterization of rdh54+, the second fission yeast Schizosaccharomyces pombe Rad54 homolog. rdh54+ shares sequence and functional homology to budding yeast RDH54/TID1. Rdh54p is...

Efficient labeling of fission yeast Schizosaccharomyces pombe with thymidine and BUdR

Hodson, Jeffrey A., Bailis, Julie M., Forsburg, Susan L.

In this paper we report the construction of a Schizosaccharomyces pombe strain that facilitates analysis of replicating DNA. The strain co-expresses the Herpes simplex virus thymidine kinase gene...

Eukaryotic MCM Proteins: Beyond Replication Initiation

Forsburg, Susan L.

The minichromosome maintenance (or MCM) protein family is composed of six related proteins that are conserved in all eukaryotes. They were first identified by genetic screens in yeast and...

Meiotic S-Phase Damage Activates Recombination without Checkpoint ArrestD⃞

Pankratz, Daniel G., Forsburg, Susan L.

Checkpoints operate during meiosis to ensure the completion of DNA synthesis and programmed recombination before the initiation of meiotic divisions. Studies in the fission yeast Schizosaccharomyces...

Suppressors of Bir1p (Survivin) Identify Roles for the Chromosomal Passenger Protein Pic1p (INCENP) and the Replication Initiation Factor Psf2p in Chromosome Segregation¶

Huang, Han-Kuei, Bailis, Julie M., Leverson, Joel D., Gómez, Eliana B., Forsburg, Susan L., Hunter, Tony

Fission yeast Bir1p/Cut17p/Pbh1p, the homolog of human Survivin, is a conserved chromosomal passenger protein that is required for cell division and cytokinesis. To study how Bir1p promotes accurate...

Schizosaccharomyces pombe mst2+ Encodes a MYST Family Histone Acetyltransferase That Negatively Regulates Telomere Silencing†

Gómez, Eliana B., Espinosa, Joaquín M., Forsburg, Susan L.

Histone acetylation and deacetylation are associated with transcriptional activity and the formation of constitutively silent heterochromatin. Increasingly, histone acetylation is also implicated in...

A Screen for Schizosaccharomyces pombe Mutants Defective in Rereplication Identifies New Alleles of rad4+, cut9+ and psf2+

Gómez, Eliana B., Angeles, Vanessa T., Forsburg, Susan L.

Fission yeast mutants defective in DNA replication have widely varying morphological phenotypes. We designed a screen for temperature-sensitive mutants defective in the process of replication...

Conserved Locus-Specific Silencing Functions of Schizosaccharomyces pombe sir2+

Freeman-Cook, Lisa L., Gómez, Eliana B., Spedale, Erik J., Marlett, John, Forsburg, Susan L., Pillus, Lorraine, ...

In Schizosaccharomyces pombe, three genes, sir2+, hst2+, and hst4+, encode members of the Sir2 family of conserved NAD+-dependent protein deacetylases. The S. pombe sir2+ gene encodes a nuclear...

Different phenotypes in vivo are associated with ATPase motif mutations in Schizosaccharomyces pombe minichromosome maintenance proteins.

Gómez, Eliana B, Catlett, Michael G, Forsburg, Susan L

The six conserved MCM proteins are essential for normal DNA replication. They share a central core of homology that contains sequences related to DNA-dependent and AAA(+) ATPases. It has been...

Multiple Domains of Fission Yeast Cdc19p (MCM2) Are Required for Its Association with the Core MCM Complex

Sherman, Daniel A., Pasion, Sally G., Forsburg, Susan L.

The members of the MCM protein family are essential eukaryotic DNA replication factors that form a six-member protein complex. In this study, we use antibodies to four MCM proteins to investigate the...

Identification of a Second Myosin-II in Schizosaccharomyces pombe:: Myp2p Is Conditionally Required for Cytokinesis

Bezanilla, Magdalena, Forsburg, Susan L., Pollard, Thomas D.

As in many eukaryotic cells, fission yeast cytokinesis depends on the assembly of an actin ring. We cloned myp2+, a myosin-II in Schizosaccharomyces pombe, conditionally required for cytokinesis....

Nuclear Localization of Schizosaccharomyces pombe Mcm2/Cdc19p Requires MCM Complex Assembly

Pasion, Sally G., Forsburg, Susan L.

The minichromosome maintenance (MCM) proteins MCM2–MCM7 are conserved eukaryotic replication factors that assemble in a heterohexameric complex. In fission yeast, these proteins are nuclear...

Schizosaccharomyces pombe Hsk1p Is a Potential Cds1p Target Required for Genome Integrity

Snaith, Hilary A., Brown, Grant W., Forsburg, Susan L.

The fission yeast Hsk1p kinase is an essential activator of DNA replication. Here we report the isolation and characterization of a novel mutant allele of the gene. Consistent with its role in the...

The Schizosaccharomyces pombe Aurora–related Kinase Ark1 Interacts with the Inner Centromere Protein Pic1 and Mediates Chromosome Segregation and Cytokinesis

Leverson, Joel D., Huang, Han-kuei, Forsburg, Susan L., Hunter, Tony

The chromosomal passenger proteins aurora-B, survivin, and inner centromere protein (INCENP) have been implicated in coordinating chromosome segregation with cell division. This work describes the...

Isolation of an essential Schizosaccharomyces pombe gene, prp31+, that links splicing and meiosis

Bishop, Danielle T., McDonald, W. Hayes, Gould, Kathleen L., Forsburg, Susan L.

We carried out a screen for mutants that arrest prior to premeiotic S phase. One of the strains we isolated contains a temperature-sensitive allele mutation in the fission yeast prp31+ gene. The...

Dynamic chromosomes

Pasion, Sally G, Gómez, Eliana B, Forsburg, Susan L

A report on the sixth biannual FASEB conference on Yeast Chromosome Structure, Replication and Segregation held in Snowmass, Colorado, 19-24 August, 2000.

Fission yeast enters a joyful new era

Gómez, Eliana B, Bailis, Julie M, Forsburg, Susan L

A report on the Second International Fission Yeast Meeting, Kyoto, Japan, 25-30 March 2002.

RNAi hushes heterochromatin

Bailis, Julie M, Forsburg, Susan L

Recent work in the fission yeast Schizosaccharomyces pombe reveals that RNA is an integral component of silent heterochromatin.

Schizosaccharomyces pombe Rdh54 (TID1) Acts with Rhp54 (RAD54) to Repair Meiotic Double-Strand Breaks V⃞

Catlett, Michael G., Forsburg, Susan L.

We report the characterization of rdh54+, the second fission yeast Schizosaccharomyces pombe Rad54 homolog. rdh54+ shares sequence and functional homology to budding yeast RDH54/TID1. Rdh54p is...

Efficient labeling of fission yeast Schizosaccharomyces pombe with thymidine and BUdR

Hodson, Jeffrey A., Bailis, Julie M., Forsburg, Susan L.

In this paper we report the construction of a Schizosaccharomyces pombe strain that facilitates analysis of replicating DNA. The strain co-expresses the Herpes simplex virus thymidine kinase gene...

Eukaryotic MCM Proteins: Beyond Replication Initiation

Forsburg, Susan L.

The minichromosome maintenance (or MCM) protein family is composed of six related proteins that are conserved in all eukaryotes. They were first identified by genetic screens in yeast and...

Meiotic S-Phase Damage Activates Recombination without Checkpoint ArrestD⃞

Pankratz, Daniel G., Forsburg, Susan L.

Checkpoints operate during meiosis to ensure the completion of DNA synthesis and programmed recombination before the initiation of meiotic divisions. Studies in the fission yeast Schizosaccharomyces...

Suppressors of Bir1p (Survivin) Identify Roles for the Chromosomal Passenger Protein Pic1p (INCENP) and the Replication Initiation Factor Psf2p in Chromosome Segregation¶

Huang, Han-Kuei, Bailis, Julie M., Leverson, Joel D., Gómez, Eliana B., Forsburg, Susan L., Hunter, Tony

Fission yeast Bir1p/Cut17p/Pbh1p, the homolog of human Survivin, is a conserved chromosomal passenger protein that is required for cell division and cytokinesis. To study how Bir1p promotes accurate...

Schizosaccharomyces pombe mst2+ Encodes a MYST Family Histone Acetyltransferase That Negatively Regulates Telomere Silencing†

Gómez, Eliana B., Espinosa, Joaquín M., Forsburg, Susan L.

Histone acetylation and deacetylation are associated with transcriptional activity and the formation of constitutively silent heterochromatin. Increasingly, histone acetylation is also implicated in...

A Screen for Schizosaccharomyces pombe Mutants Defective in Rereplication Identifies New Alleles of rad4+, cut9+ and psf2+

Gómez, Eliana B., Angeles, Vanessa T., Forsburg, Susan L.

Fission yeast mutants defective in DNA replication have widely varying morphological phenotypes. We designed a screen for temperature-sensitive mutants defective in the process of replication...

Conserved Locus-Specific Silencing Functions of Schizosaccharomyces pombe sir2+

Freeman-Cook, Lisa L., Gómez, Eliana B., Spedale, Erik J., Marlett, John, Forsburg, Susan L., Pillus, Lorraine, ...

In Schizosaccharomyces pombe, three genes, sir2+, hst2+, and hst4+, encode members of the Sir2 family of conserved NAD+-dependent protein deacetylases. The S. pombe sir2+ gene encodes a nuclear...

Different phenotypes in vivo are associated with ATPase motif mutations in Schizosaccharomyces pombe minichromosome maintenance proteins.

Gómez, Eliana B, Catlett, Michael G, Forsburg, Susan L

The six conserved MCM proteins are essential for normal DNA replication. They share a central core of homology that contains sequences related to DNA-dependent and AAA(+) ATPases. It has been...

Minichromosome Maintenance Proteins Interact with Checkpoint and Recombination Proteins To Promote S-Phase Genome Stability▿ †

Bailis, Julie M., Luche, Douglas D., Hunter, Tony, Forsburg, Susan L.

The minichromosome maintenance (MCM) complex plays essential, conserved roles throughout DNA synthesis: first, as a component of the prereplication complex at origins and, then, as a helicase...

Schizosaccharomyces pombe Histone Acetyltransferase Mst1 (KAT5) Is an Essential Protein Required for Damage Response and Chromosome Segregation

Gómez, Eliana B., Nugent, Rebecca L., Laria, Sebastián, Forsburg, Susan L.

Schizosaccharomyces pombe Mst1 is a member of the MYST family of histone acetyltransferases and is the likely ortholog of Saccharomyces cerevisiae Esa1 and human Tip60 (KAT5). We have isolated a...