The nucleosome: from wallflower to Queen of the Ball (2001)
Cubizolles, Fabien, Gasser, Susan M
A report on the FASEB meeting on Chromatin and Transcription, Snowmass, USA, 7-12 July 2001.
Functional Characterization of the N Terminus of Sir3p
Gotta, Monica, Palladino, Francesca, Gasser, Susan M.
Silent information regulator 3 is an essential component of the Saccharomyces cerevisiae silencing complex that functions at telomeres and the silent mating-type loci, HMR and HML. We show that...
Duncker, Bernard P., Pasero, Philippe, Braguglia, Diego, Heun, Patrick, Weinreich, Michael, Gasser, Susan M.
Nuclear extracts from Saccharomyces cerevisiae cells synchronized in S phase support the semiconservative replication of supercoiled plasmids in vitro. We examined the dependence of this reaction on...
Duncker, Bernard P., Shimada, Kenji, Tsai-Pflugfelder, Monika, Pasero, Philippe, Gasser, Susan M.
The Dbf4/Cdc7 kinase acts at the level of individual origins to promote the initiation of DNA replication. We demonstrate through both immunoprecipitation and two-hybrid assays that a domain...
The nucleosome: from wallflower to Queen of the Ball
Cubizolles, Fabien, Gasser, Susan M
A report on the FASEB meeting on Chromatin and Transcription, Snowmass, USA, 7-12 July 2001.
Perrod, Séverine, Cockell, Moira M., Laroche, Thierry, Renauld, Hubert, Ducrest, Anne-Lyse, Bonnard, Claude, ...
In budding yeast, the silent information regulator Sir2p is a nuclear NAD-dependent deacetylase that is essential for both telomeric and rDNA silencing. All eukaryotic species examined to date have...
DNA polymerase stabilization at stalled replication forks requires Mec1 and the RecQ helicase Sgs1
Cobb, Jennifer A., Bjergbaek, Lotte, Shimada, Kenji, Frei, Christian, Gasser, Susan M.
To ensure proper replication and segregation of the genome, eukaryotic cells have evolved surveillance systems that monitor and react to impaired replication fork progression. In budding yeast, the...
ORC and the intra-S-phase checkpoint: a threshold regulates Rad53p activation in S phase
Shimada, Kenji, Pasero, Philippe, Gasser, Susan M.
The intra-S-phase checkpoint in yeast responds to stalled replication forks by activating the ATM-like kinase Mec1 and the CHK2-related kinase Rad53, which in turn inhibit spindle elongation and late...
Vilain, Nathalie, Tsai-Pflugfelder, Monika, Benoit, Audrey, Gasser, Susan M., Leroy, Didier
Epipodophyllotoxins are effective antitumour drugs that trap eukaryotic DNA topoisomerase II in a covalent complex with DNA. Based on DNA cleavage assays, the mode of interaction of these drugs was...
Frei, Christian, Gasser, Susan M.
We have examined the cellular function of Sgs1p, a nonessential yeast DNA helicase, homologs of which are implicated in two highly debilitating hereditary human diseases (Werner's and Bloom's...
Pasero, Philippe, Duncker, Bernard P., Schwob, Etienne, Gasser, Susan M.
Using a reconstituted DNA replication assay from yeast, we demonstrate that two kinase complexes are essential for the promotion of replication in vitro. An active Clb/Cdc28 kinase complex, or its...
Separation of silencing from perinuclear anchoring functions in yeast Ku80, Sir4 and Esc1 proteins
Taddei, Angela, Hediger, Florence, Neumann, Frank R, Bauer, Christoph, Gasser, Susan M
In budding yeast, the nuclear periphery forms a subcompartment in which telomeres cluster and SIR proteins concentrate. To identify the proteins that mediate chromatin anchorage to the nuclear...
Bystricky, Kerstin, Heun, Patrick, Gehlen, Lutz, Langowski, Jörg, Gasser, Susan M.
Little is known about how chromatin folds in its native state. Using optimized in situ hybridization and live imaging techniques have determined compaction ratios and fiber flexibility for interphase...
Mechanistically distinct roles for Sgs1p in checkpoint activation and replication fork maintenance
Bjergbaek, Lotte, Cobb, Jennifer A, Tsai-Pflugfelder, Monica, Gasser, Susan M
The RecQ helicase Sgs1p forms a complex with the type 1 DNA topoisomerase Top3p that resolves double Holliday junctions resulting from Rad51-mediated exchange. We find, however, that Sgs1p functions...
Ku-deficient yeast strains exhibit alternative states of silencing competence
Maillet, Laurent, Gaden, Florence, Brevet, Vanessa, Fourel, Geneviève, Martin, Sophie G., Dubrana, Karine, ...
In Saccharomyces cerevisiae, efficient silencer function requires telomere proximity, i.e. compartments of the nucleoplasm enriched in silencing factors. Accordingly, silencers located far from...
Intracellular trafficking of yeast telomerase components
Teixeira, M. Teresa, Förstemann, Klaus, Gasser, Susan M., Lingner, Joachim
Telomerase uses an internal RNA moiety as template for the synthesis of telomere repeats. In Saccharomyces cerevisiae, the telomerase holoenzyme contains the telomerase reverse transcriptase subunit...
A molecular dissection of nuclear function
Spector, David L., Gasser, Susan M.
Conference on The Dynamic Nucleus: Questions and Implications
Cobb, Jennifer A., Schleker, Thomas, Rojas, Vanesa, Bjergbaek, Lotte, Tercero, José Antonio, Gasser, Susan M.
The yeast checkpoint kinases Mec1 and Rad53 are required for genomic stability in the presence of replicative stress. When replication forks stall, the stable maintenance of replisome components...
Functional Characterization of the N Terminus of Sir3p
Gotta, Monica, Palladino, Francesca, Gasser, Susan M.
Silent information regulator 3 is an essential component of the Saccharomyces cerevisiae silencing complex that functions at telomeres and the silent mating-type loci, HMR and HML. We show that...
Duncker, Bernard P., Pasero, Philippe, Braguglia, Diego, Heun, Patrick, Weinreich, Michael, Gasser, Susan M.
Nuclear extracts from Saccharomyces cerevisiae cells synchronized in S phase support the semiconservative replication of supercoiled plasmids in vitro. We examined the dependence of this reaction on...
Duncker, Bernard P., Shimada, Kenji, Tsai-Pflugfelder, Monika, Pasero, Philippe, Gasser, Susan M.
The Dbf4/Cdc7 kinase acts at the level of individual origins to promote the initiation of DNA replication. We demonstrate through both immunoprecipitation and two-hybrid assays that a domain...
The nucleosome: from wallflower to Queen of the Ball
Cubizolles, Fabien, Gasser, Susan M
A report on the FASEB meeting on Chromatin and Transcription, Snowmass, USA, 7-12 July 2001.
Perrod, Séverine, Cockell, Moira M., Laroche, Thierry, Renauld, Hubert, Ducrest, Anne-Lyse, Bonnard, Claude, ...
In budding yeast, the silent information regulator Sir2p is a nuclear NAD-dependent deacetylase that is essential for both telomeric and rDNA silencing. All eukaryotic species examined to date have...
DNA polymerase stabilization at stalled replication forks requires Mec1 and the RecQ helicase Sgs1
Cobb, Jennifer A., Bjergbaek, Lotte, Shimada, Kenji, Frei, Christian, Gasser, Susan M.
To ensure proper replication and segregation of the genome, eukaryotic cells have evolved surveillance systems that monitor and react to impaired replication fork progression. In budding yeast, the...
ORC and the intra-S-phase checkpoint: a threshold regulates Rad53p activation in S phase
Shimada, Kenji, Pasero, Philippe, Gasser, Susan M.
The intra-S-phase checkpoint in yeast responds to stalled replication forks by activating the ATM-like kinase Mec1 and the CHK2-related kinase Rad53, which in turn inhibit spindle elongation and late...
Vilain, Nathalie, Tsai-Pflugfelder, Monika, Benoit, Audrey, Gasser, Susan M., Leroy, Didier
Epipodophyllotoxins are effective antitumour drugs that trap eukaryotic DNA topoisomerase II in a covalent complex with DNA. Based on DNA cleavage assays, the mode of interaction of these drugs was...
Frei, Christian, Gasser, Susan M.
We have examined the cellular function of Sgs1p, a nonessential yeast DNA helicase, homologs of which are implicated in two highly debilitating hereditary human diseases (Werner's and Bloom's...
Pasero, Philippe, Duncker, Bernard P., Schwob, Etienne, Gasser, Susan M.
Using a reconstituted DNA replication assay from yeast, we demonstrate that two kinase complexes are essential for the promotion of replication in vitro. An active Clb/Cdc28 kinase complex, or its...
Separation of silencing from perinuclear anchoring functions in yeast Ku80, Sir4 and Esc1 proteins
Taddei, Angela, Hediger, Florence, Neumann, Frank R, Bauer, Christoph, Gasser, Susan M
In budding yeast, the nuclear periphery forms a subcompartment in which telomeres cluster and SIR proteins concentrate. To identify the proteins that mediate chromatin anchorage to the nuclear...
Bystricky, Kerstin, Heun, Patrick, Gehlen, Lutz, Langowski, Jörg, Gasser, Susan M.
Little is known about how chromatin folds in its native state. Using optimized in situ hybridization and live imaging techniques have determined compaction ratios and fiber flexibility for interphase...
Mechanistically distinct roles for Sgs1p in checkpoint activation and replication fork maintenance
Bjergbaek, Lotte, Cobb, Jennifer A, Tsai-Pflugfelder, Monica, Gasser, Susan M
The RecQ helicase Sgs1p forms a complex with the type 1 DNA topoisomerase Top3p that resolves double Holliday junctions resulting from Rad51-mediated exchange. We find, however, that Sgs1p functions...
Ku-deficient yeast strains exhibit alternative states of silencing competence
Maillet, Laurent, Gaden, Florence, Brevet, Vanessa, Fourel, Geneviève, Martin, Sophie G., Dubrana, Karine, ...
In Saccharomyces cerevisiae, efficient silencer function requires telomere proximity, i.e. compartments of the nucleoplasm enriched in silencing factors. Accordingly, silencers located far from...
Intracellular trafficking of yeast telomerase components
Teixeira, M. Teresa, Förstemann, Klaus, Gasser, Susan M., Lingner, Joachim
Telomerase uses an internal RNA moiety as template for the synthesis of telomere repeats. In Saccharomyces cerevisiae, the telomerase holoenzyme contains the telomerase reverse transcriptase subunit...
Cobb, Jennifer A., Schleker, Thomas, Rojas, Vanesa, Bjergbaek, Lotte, Tercero, José Antonio, Gasser, Susan M.
The yeast checkpoint kinases Mec1 and Rad53 are required for genomic stability in the presence of replicative stress. When replication forks stall, the stable maintenance of replisome components...
A molecular dissection of nuclear function
Spector, David L., Gasser, Susan M.
Conference on The Dynamic Nucleus: Questions and Implications
Measuring Limits of Telomere Movement on Nuclear Envelope
Rosa, Angelo, Maddocks, John H., Neumann, Frank R., Gasser, Susan M., Stasiak, Andrzej
The dynamic behavior of the decondensed chromatin can be monitored by real-time fluorescence confocal microscopy. It can be observed that different chromosomal sites enjoy different degrees of...
Subtelomeric factors antagonize telomere anchoring and Tel1-independent telomere length regulation
Hediger, Florence, Berthiau, Anne-Sophie, Van Houwe, Griet, Gilson, Eric, Gasser, Susan M
Yeast telomeres are anchored at the nuclear envelope (NE) through redundant pathways that require the telomere-binding factors yKu and Sir4. Significant variation is observed in the efficiency with...
Meister, Peter, Taddei, Angela, Ponti, Aaron, Baldacci, Giuseppe, Gasser, Susan M
Although the molecular enzymology of DNA replication is well characterised, how and why it occurs in discrete nuclear foci is unclear. Using fission yeast, we show that replication takes place in a...
Bystricky, Kerstin, Laroche, Thierry, Van Houwe, Griet, Blaszczyk, Marek, Gasser, Susan M.
Long-range chromosome organization is known to influence nuclear function. Budding yeast centromeres cluster near the spindle pole body, whereas telomeres are grouped in five to eight perinuclear...
Temporal separation of replication and recombination requires the intra-S checkpoint
Meister, Peter, Taddei, Angela, Vernis, Laurence, Poidevin, Mickaël, Gasser, Susan M., Baldacci, Giuseppe
In response to DNA damage and replication pausing, eukaryotes activate checkpoint pathways that prevent genomic instability by coordinating cell cycle progression with DNA repair. The intra-S-phase...
The Positioning and Dynamics of Origins of Replication in the Budding Yeast Nucleus
Heun, Patrick, Laroche, Thierry, Raghuraman, M.K., Gasser, Susan M.
We have analyzed the subnuclear position of early- and late-firing origins of DNA replication in intact yeast cells using fluorescence in situ hybridization and green fluorescent protein...
Controlled exchange of chromosomal arms reveals principles driving telomere interactions in yeast
Schober, Heiko, Kalck, Véronique, Vega-Palas, Miguel A., Van Houwe, Griet, Sage, Daniel, Unser, Michael, ...
The 32 telomeres in the budding yeast genome cluster in three to seven perinuclear foci. Although individual telomeres and telomeric foci are in constant motion, preferential juxtaposition of some...
Distinct roles for SWR1 and INO80 chromatin remodeling complexes at chromosomal double-strand breaks
Van Attikum, Haico, Fritsch, Olivier, Gasser, Susan M
INO80 and SWR1 are two closely related ATP-dependent chromatin remodeling complexes that share several subunits. Ino80 was reported to be recruited to the HO endonuclease-induced double-strand break...