Shingler, William H., Chikoti, Priscilla, Kingsman, Susan M., Harrop, Richard
The cancer vaccine TroVax, modified vaccinia Ankara encoding the tumor-associated antigen 5T4, has been tested in phase I and II studies in colorectal cancer patients. Monitoring of 5T4-specific...
Ralph, G Scott, Walmsley, Lucy E, Mitrophanous, Kyriacos A, Kingsman, Susan M, ...
Amyotrophic lateral sclerosis (ALS) causes adult-onset, progressive motor neuron degeneration in the brain and spinal cord, resulting in paralysis and death three to five years after onset in most...
Mazarakis, Nicholas D., Azzouz, Mimoun, Rohll, Jonathan B., Ellard, Fiona M., Wilkes, Fraser J., Olsen, Anna L., ...
In this report it is demonstrated for the first time that rabies-G envelope of the rabies virus is sufficient to confer retrograde axonal transport to a heterologous virus/vector. After delivery...
Soneoka, Yuko, Cannon, Paula M., Ramsdale, Emma E., Griffiths, Joanne C., Romano, Gaetano, Kingsman, Susan M., ...
We have constructed a series of MLV-based retroviral vectors and packaging components expressed from the CMV promoter and carried on plasmids containing SV40 origins of replication. These two...
Braddock, Martin, Muckenthaler, Martina, White, Michael R.H., Thorbum, Andrew M., Sommerville, John, Kingsman, Alan J., ...
The translation of a capped, polyadenylated RNA after Injection Into the nucleus of Xenopus oocytes occurs only If the RNA contains an Intron. A single point mutation In the splice donor site...
Identification of a novel HIV-1 TAR RNA bulge binding protein (1994)
Baker, Bernadette, Muckenthaler, Martina, Vives, Eric, Blanchard, Andy, Braddock, Martin, Nacken, W., ...
The Tat protein binds to TAR RNA to stimulate the expression of the human immunodeficiency virus type 1 (HIV-1) genome. Tat is an 86 amino acid protein that contains a short region of basic residues...
Characterisation of the DNA binding domain of the yeast RAP1 protein (1990)
Henry, Yves A.L., Chambers, Alistair, Tsang, Jimmy S.H., Kingsman, Alan J., Kingsman, Susan M.
The 827 amino acid yeast RAP1 protein interacts with DNA to regulate gene expression at numerous unrelated loci in the yeast genome. By a combination of amino, carboxy and internal deletions, we have...
Chambers, Alistair, Stanway, Clive, Tsang, Jimmy S.H., Henry, Yves, Kingsman, Alan J., Kingsman, Susan M.
The UAS of the yeast gene encoding the glycolytic enzyme phosphoglycerate kinase (PGK) contains several different sequence elements involved in transcriptional activation. These elements include the...
Tsang, Jimmy S.H., Henry, Yves A.L., Chambers, Alistair, Kingsman, Alan J., Kingsman, Susan M.
Yeast repressor activator protein 1 (RAP 1) binds in vitro to specific DNA sequences that are found in diverse genetic elements. Expression of the yeast phosphoglycerate kinase gene (PGK) requires...
Stanway, Clive A., Chambers, Alistair, Kingsman, Alan J., Kingsman, Susan M.
The upstream activator (UAS) of the yeast PGK gene comprises three different sequence elements. These are 1) a region of strong protein binding called the YFP, 2) three repeats of the motif CTTCC and...
Adams, Sally E., Johnson, Ian D., Braddock, Martin, Kingsman, Alan J., Kingsman, Susan M., Edwards, R.Mark
The synthesis of a gene for the HIV TAT protein is described using a novel approach that capitalises on the ability to synthesise oligonucleotides of greater than 100 bp in length. It involves the...
The UAS of the yeast PGK gene is composed of multiple functional elements (1988)
Chambers, Alistair, Stanway, Clive, Kingsman, Alan J., Kingsman, Susan M.
The UAS ( upstream activator sequence ) of the yeast PGK gene contains a transcriptional activator domain located between bases −479 and −402 upstream from the initiating ATG. This region of the...
Upstream and downstream transcriptional control signals in the yeast retrotransposon, TY (1988)
Fulton, Alexandra M., Rathjen, Peter D., Kingsman, Susan M., Kingsman, Alan J.
The yeast retrotransposon, Ty, shares many structural and functional features with retroviral proviruses. These include production of a terminally redundant major transcript. There are also two leas...
The UAS of the yeast PGK gene contains functionally distinct domains (1987)
Stanway, Clive, Mellor, Jane, Ogden, Jill E., Kingsman, Alan J., Kingsman, Susan M.
The upstream activation site (UAS) of the yeast phosphoglycerate kinase gene (PGK) has been localised by deletion analysis (1). Here we show that the UASPGK contains two functionally distinct...
The productton of hybrid Ty: IFN virus-like particles in yeast (1987)
Malim, Michael H., Adams, Sally E., Gull, Keith, Kingsman, Alan J., Kingsman, Susan M.
The yeast retrotransposon Ty encodes proteins that assemble into virus-like particles (Ty-VLPs) which can be readily purified (1, 2). We have recently shown that expression of the p1 protein encoded...
Wilson, Wilma, Malim, Michael H., Mellor, Jane, Kingsman, Alan J., Kingsman, Susan M.
The Ty element of yeast is a member of a class of eukaryotic transposons which bear a striking resemblance to retroviral proviruses in their structure and expression strategies (1,2). A direct...
Drosophila ARSs contain the yeast ARS consensus sequence and a replication enhancer (1986)
Mills, John S., Kingsman, Alan J., Kingsman, Susan M.
A number of restriction fragments that function as autonomously replicating sequences (ARSs) in yeast have been isolated from Drosophila melanogaster DNA. The behaviour in yeast of plasmids...
Mellor, Jane, Fulton, Alexandra M., Dobson, Melanie J., Roberts, Nichola A., Wilson, Wilma, Kingsman, Alan J., ...
Two new Ty determined proteins have been identified by placing a Ty transcriptional unit under the control of a high efficiency yeast expression vector. One of these proteins is the product of a...
Fulton, Alexandra M., Mellor, Jane, Dobson, Melanie J., Chester, John, Warmington, John R., Indge, Keith J., ...
The Ty transposable elements of Saccharomyces cerevisiae form a heterogeneous family within which two broad structural classes (I and II) exist. The two classes differ by two large substitutions and...
Minimal Requirement for a Lentivirus Vector Based on Human Immunodeficiency Virus Type 1
Kim, V. Narry, Mitrophanous, Kyriacos, Kingsman, Susan M., Kingsman, Alan J.
The use of human immunodeficiency virus vectors for gene therapy is hampered by concern over their safety. This concern might be ameliorated, in part, if the viral accessory genes and proteins could...
Split-Intron Retroviral Vectors: Enhanced Expression with Improved Safety
Ismail, Said I., Kingsman, Susan M., Kingsman, Alan J., Uden, Mark
The inclusion of retrovirus-derived introns within retrovirus-based expression vectors leads to a fraction of the resulting transcripts being spliced. Such splicing has been shown to markedly improve...
Kotsopoulou, Ekaterini, Kim, V. Narry, Kingsman, Alan J., Kingsman, Susan M., Mitrophanous, Kyriacos A.
The human immunodeficiency virus (HIV) genome is AU rich, and this imparts a codon bias that is quite different from the one used by human genes. The codon usage is particularly marked for the gag,...
Use of Intron-Disrupted Polyadenylation Sites To Enhance Expression and Safety of Retroviral Vectors
Ismail, Said I., Rohll, Jonathan B., Kingsman, Susan M., Kingsman, Alan J., Uden, Mark
Normal mRNA polyadenylation signals are composed of an AAUAAA motif and G/U box spaced 20 to 30 bp apart. If this spacing is increased further, then polyadenylation is disrupted. Previously it has...
Kingsman, Susan M., Smith, Mark D., Samuel, Charles E.
The effect of interferon treatment on proteins synthesized in simian virus 40 (SV40)-infected cells in the presence of cytosine arabinoside was investigated. The following results were obtained: (i)...
Lentivector-mediated SMN replacement in a mouse model of spinal muscular atrophy
Azzouz, Mimoun, Le, Thanh, Ralph, G. Scott, Walmsley, Lucy, Monani, Umrao R., Lee, Debbie C.P., ...
Spinal muscular atrophy (SMA) is a frequent recessive autosomal disorder. It is caused by mutations or deletion of the telomeric copy of the survival motor neuron (SMN) gene, leading to depletion in...
Minimal Requirement for a Lentivirus Vector Based on Human Immunodeficiency Virus Type 1
Kim, V. Narry, Mitrophanous, Kyriacos, Kingsman, Susan M., Kingsman, Alan J.
The use of human immunodeficiency virus vectors for gene therapy is hampered by concern over their safety. This concern might be ameliorated, in part, if the viral accessory genes and proteins could...
Split-Intron Retroviral Vectors: Enhanced Expression with Improved Safety
Ismail, Said I., Kingsman, Susan M., Kingsman, Alan J., Uden, Mark
The inclusion of retrovirus-derived introns within retrovirus-based expression vectors leads to a fraction of the resulting transcripts being spliced. Such splicing has been shown to markedly improve...
Kotsopoulou, Ekaterini, Kim, V. Narry, Kingsman, Alan J., Kingsman, Susan M., Mitrophanous, Kyriacos A.
The human immunodeficiency virus (HIV) genome is AU rich, and this imparts a codon bias that is quite different from the one used by human genes. The codon usage is particularly marked for the gag,...
Use of Intron-Disrupted Polyadenylation Sites To Enhance Expression and Safety of Retroviral Vectors
Ismail, Said I., Rohll, Jonathan B., Kingsman, Susan M., Kingsman, Alan J., Uden, Mark
Normal mRNA polyadenylation signals are composed of an AAUAAA motif and G/U box spaced 20 to 30 bp apart. If this spacing is increased further, then polyadenylation is disrupted. Previously it has...
Kingsman, Susan M., Smith, Mark D., Samuel, Charles E.
The effect of interferon treatment on proteins synthesized in simian virus 40 (SV40)-infected cells in the presence of cytosine arabinoside was investigated. The following results were obtained: (i)...
Lentivector-mediated SMN replacement in a mouse model of spinal muscular atrophy
Azzouz, Mimoun, Le, Thanh, Ralph, G. Scott, Walmsley, Lucy, Monani, Umrao R., Lee, Debbie C.P., ...
Spinal muscular atrophy (SMA) is a frequent recessive autosomal disorder. It is caused by mutations or deletion of the telomeric copy of the survival motor neuron (SMN) gene, leading to depletion in...