Therapeutic intervention based on protein prenylation and associated modifications (2006)
Gelb, Michael H., Brunsveld, Lucas, Hrycyna, Christine A., Michaelis, Susan, Tamanoi, Fuyuhiko, Van Voorhis, Wesley C., ...
Gregory Huyer, Gaby L. Longsworth, Deborah L. Mason, Monica P. Mallampalli, J. Michael, Robin L. Wright, ...
endoplasmic reticulum-associated compartment (ERAC)
Schmidt, Walter K., Tam, Amy, Fujimura-Kamada, Konomi, Michaelis, Susan
Proteins terminating in the CAAX motif, for example Ras and the yeast a-factor mating pheromone, are prenylated, trimmed of their last three amino acids, and carboxyl-methylated. The enzymes that...
Romano, Julia D., Schmidt, Walter K., Michaelis, Susan
Eukaryotic proteins containing a C-terminal CAAX motif undergo a series of posttranslational CAAX-processing events that include isoprenylation, C-terminal proteolytic cleavage, and carboxyl...
Loayza, Diego, Tam, Amy, Schmidt, Walter K., Michaelis, Susan
We are studying the intracellular trafficking of the multispanning membrane protein Ste6p, the a-factor transporter in Saccharomyces cerevisiae and a member of the ATP-binding cassette superfamily of...
Zhang, Yimao, Nijbroek, Gaby, Sullivan, Mara L., McCracken, Ardythe A., Watkins, Simon C., Michaelis, Susan, ...
Membrane and secretory proteins fold in the endoplasmic reticulum (ER), and misfolded proteins may be retained and targeted for ER-associated protein degradation (ERAD). To elucidate the mechanism by...
Romano, Julia D., Michaelis, Susan
Eukaryotic proteins that terminate in a CaaX motif undergo three processing events: isoprenylation, C-terminal proteolytic cleavage, and carboxyl methylation. In Saccharomyces cerevisiae, the latter...
Loayza, Diego, Michaelis, Susan
Ste6p, the a-factor transporter in Saccharomyces cerevisiae, is a multispanning membrane protein with 12 transmembrane spans and two cytosolic ATP binding domains. Ste6p belongs to the ATP binding...
Sharma, Kailash Gulshan, Mason, Deborah L., Liu, Guosheng, Rea, Philip A., Bachhawat, Anand K., Michaelis, Susan
Saccharomyces cerevisiae Bpt1p is an ATP-binding cassette (ABC) protein that belongs to the MRP subfamily and is a close homologue of the glutathione conjugate (GS conjugate) transporter Ycf1p. The...
Bergo, Martin O., Gavino, Bryant, Ross, Jed, Schmidt, Walter K., Hong, Christine, Kendall, Lonnie V., ...
Zmpste24 is an integral membrane metalloproteinase of the endoplasmic reticulum. Biochemical studies of tissues from Zmpste24-deficient mice (Zmpste24−/−) have indicated a role for Zmpste24 in...
Mason, Deborah L., Michaelis, Susan
Ycf1p is the prototypical member of the yeast multidrug resistance-associated protein (MRP) subfamily of ATP-binding cassette (ABC) transporters. Ycf1p resides in the vacuolar membrane and mediates...
Mason, Deborah L., Mallampalli, Monica P., Huyer, Gregory, Michaelis, Susan
Ycf1p, a member of the yeast multidrug resistance-associated protein (MRP) subfamily of ATP-binding cassette proteins, is a vacuolar membrane transporter that confers resistance to a variety of toxic...
Huyer, Gregory, Longsworth, Gaby L., Mason, Deborah L., Mallampalli, Monica P., McCaffery, J. Michael, Wright, Robin L., ...
The folding of nascent secretory and membrane proteins is monitored by the endoplasmic reticulum (ER) quality control system. Misfolded proteins are retained in the ER and can be removed by...
Prelamin A endoproteolytic processing in vitro by recombinant Zmpste24
Corrigan, Douglas P., Kuszczak, Danuta, Rusinol, Antonio E., Thewke, Douglas P., Hrycyna, Christine A., Michaelis, Susan, ...
The nuclear lamins form a karyoskeleton providing structural rigidity to the nucleus. One member of the lamin family, lamin A, is first synthesized as a 74 kDa precursor, prelamin A. After the...
Mallampalli, Monica P., Huyer, Gregory, Bendale, Pravin, Gelb, Michael H., Michaelis, Susan
Hutchinson-Gilford progeria syndrome (HGPS) is a devastating premature aging disease resulting from a mutation in the LMNA gene, which encodes nuclear lamins A and C. Lamin A is synthesized as a...
Schmidt, Walter K., Tam, Amy, Fujimura-Kamada, Konomi, Michaelis, Susan
Proteins terminating in the CAAX motif, for example Ras and the yeast a-factor mating pheromone, are prenylated, trimmed of their last three amino acids, and carboxyl-methylated. The enzymes that...
Romano, Julia D., Schmidt, Walter K., Michaelis, Susan
Eukaryotic proteins containing a C-terminal CAAX motif undergo a series of posttranslational CAAX-processing events that include isoprenylation, C-terminal proteolytic cleavage, and carboxyl...
Loayza, Diego, Tam, Amy, Schmidt, Walter K., Michaelis, Susan
We are studying the intracellular trafficking of the multispanning membrane protein Ste6p, the a-factor transporter in Saccharomyces cerevisiae and a member of the ATP-binding cassette superfamily of...
Zhang, Yimao, Nijbroek, Gaby, Sullivan, Mara L., McCracken, Ardythe A., Watkins, Simon C., Michaelis, Susan, ...
Membrane and secretory proteins fold in the endoplasmic reticulum (ER), and misfolded proteins may be retained and targeted for ER-associated protein degradation (ERAD). To elucidate the mechanism by...
Romano, Julia D., Michaelis, Susan
Eukaryotic proteins that terminate in a CaaX motif undergo three processing events: isoprenylation, C-terminal proteolytic cleavage, and carboxyl methylation. In Saccharomyces cerevisiae, the latter...
Loayza, Diego, Michaelis, Susan
Ste6p, the a-factor transporter in Saccharomyces cerevisiae, is a multispanning membrane protein with 12 transmembrane spans and two cytosolic ATP binding domains. Ste6p belongs to the ATP binding...
Sharma, Kailash Gulshan, Mason, Deborah L., Liu, Guosheng, Rea, Philip A., Bachhawat, Anand K., Michaelis, Susan
Saccharomyces cerevisiae Bpt1p is an ATP-binding cassette (ABC) protein that belongs to the MRP subfamily and is a close homologue of the glutathione conjugate (GS conjugate) transporter Ycf1p. The...
Bergo, Martin O., Gavino, Bryant, Ross, Jed, Schmidt, Walter K., Hong, Christine, Kendall, Lonnie V., ...
Zmpste24 is an integral membrane metalloproteinase of the endoplasmic reticulum. Biochemical studies of tissues from Zmpste24-deficient mice (Zmpste24−/−) have indicated a role for Zmpste24 in...
Mason, Deborah L., Michaelis, Susan
Ycf1p is the prototypical member of the yeast multidrug resistance-associated protein (MRP) subfamily of ATP-binding cassette (ABC) transporters. Ycf1p resides in the vacuolar membrane and mediates...
Mason, Deborah L., Mallampalli, Monica P., Huyer, Gregory, Michaelis, Susan
Ycf1p, a member of the yeast multidrug resistance-associated protein (MRP) subfamily of ATP-binding cassette proteins, is a vacuolar membrane transporter that confers resistance to a variety of toxic...
Huyer, Gregory, Longsworth, Gaby L., Mason, Deborah L., Mallampalli, Monica P., McCaffery, J. Michael, Wright, Robin L., ...
The folding of nascent secretory and membrane proteins is monitored by the endoplasmic reticulum (ER) quality control system. Misfolded proteins are retained in the ER and can be removed by...
Prelamin A endoproteolytic processing in vitro by recombinant Zmpste24
Corrigan, Douglas P., Kuszczak, Danuta, Rusinol, Antonio E., Thewke, Douglas P., Hrycyna, Christine A., Michaelis, Susan, ...
The nuclear lamins form a karyoskeleton providing structural rigidity to the nucleus. One member of the lamin family, lamin A, is first synthesized as a 74 kDa precursor, prelamin A. After the...
Mallampalli, Monica P., Huyer, Gregory, Bendale, Pravin, Gelb, Michael H., Michaelis, Susan
Hutchinson-Gilford progeria syndrome (HGPS) is a devastating premature aging disease resulting from a mutation in the LMNA gene, which encodes nuclear lamins A and C. Lamin A is synthesized as a...
Huyer, Gregory, Kistler, Amy, Nouvet, Franklin J., George, Carolyn M., Boyle, Meredith L., Michaelis, Susan
The Saccharomyces cerevisiae mating pheromone a-factor provides a paradigm for understanding the biogenesis of prenylated fungal pheromones. The biogenesis of a-factor involves multiple steps: (i)...
Biogenesis of the Saccharomyces cerevisiae Mating Pheromone a-Factor
Chen, Peng, Sapperstein, Stephanie K., Choi, Jonathan D., Michaelis, Susan
The Saccharomyces cerevisiae mating pheromone a-factor is a prenylated and carboxyl methylated extracellular peptide signaling molecule. Biogenesis of the a-factor precursor proceeds via a...
Fujimura-Kamada, Konomi, Nouvet, Franklin J., Michaelis, Susan
Many secreted bioactive signaling molecules, including the yeast mating pheromones a-factor and α-factor, are initially synthesized as precursors requiring multiple intracellular processing enzymes...
Tam, Amy, Nouvet, Franklin J., Fujimura-Kamada, Konomi, Slunt, Hilda, Sisodia, Sangram S., Michaelis, Susan
Maturation of the Saccharomyces cerevisiae a-factor precursor involves COOH-terminal CAAX processing (prenylation, AAX tripeptide proteolysis, and carboxyl methylation) followed by cleavage of an...
Analysis of Prelamin A Biogenesis Reveals the Nucleus to be a CaaX Processing Compartment
Barrowman, Jemima, Hamblet, Corinne, George, Carolyn M., Michaelis, Susan
Proteins establish and maintain a distinct intracellular localization by means of targeting, retention, and retrieval signals, ensuring most proteins reside predominantly in one cellular location....
Metzger, Meredith Boyle, Michaelis, Susan
ER quality control (ERQC) prevents the exit of misfolded secretory and membrane proteins from the ER. A critical aspect of ERQC is a transcriptional response called the unfolded protein response...
Paumi, Christian M., Chuk, Matthew, Chevelev, Igor, Stagljar, Igor, Michaelis, Susan
The yeast vacuolar membrane protein Ycf1p and its mammalian counterpart, MRP1, belong to the ABCC subfamily of ATP-binding cassette (ABC) transporters that rid cells of toxic endogenous and...
Degradation of a Cytosolic Protein Requires Endoplasmic Reticulum-associated Degradation Machinery*
Metzger, Meredith Boyle, Maurer, Matthew J., Dancy, Beverley M., Michaelis, Susan
Protein misfolding is monitored by a variety of cellular “quality control” systems. Endoplasmic reticulum (ER) quality control handles misfolded secretory and membrane proteins and is well...