Biodegradable Vaccine Microcapsules for Systemic and Mucosal Immunization against VEE. (1998)
The overall goal of this contract effort was to determine the effectiveness of microencapsulated vaccines compared to the conventional vaccines in inducing protective immune responses against...
Barletta, Raul G., Michalek, Suzanne M., Curtis, Roy
The Streptococcus mutans serotype c gtfA gene encodes a 55-kilodalton protein which catalyzes the synthesis of a small glucan (1.5 kilodaltons) from sucrose (J. P. Robeson, R. G. Barletta, and R....
Childers, Noel K., Tong, Giang, Mitchell, Stephen, Kirk, Katharine, Russell, Michael W., Michalek, Suzanne M.
Recent attention to mucosal immunization strategies has been focused on the nasal route for vaccine delivery. This study was designed to determine the effectiveness of a liposome-protein vaccine...
Jespersgaard, Christina, Hajishengallis, George, Greenway, Terrence E., Smith, Daniel J., Russell, Michael W., Michalek, Suzanne M.
Glucosyltransferase (GTF) enzymes of mutans streptococci are considered virulence factors due to their ability to synthesize adhesive glucans, which facilitate cell-to-cell adherence and...
Virulence of a spaP Mutant of Streptococcus mutans in a Gnotobiotic Rat Model
Crowley, Paula J., Brady, L. Jeannine, Michalek, Suzanne M., Bleiweis, Arnold S.
Streptococcus mutans, the principal etiologic agent of dental caries in humans, possesses a variety of virulence traits that enable it to establish itself in the oral cavity and initiate disease. A...
Katz, Jannet, Black, Kelley P., Michalek, Suzanne M.
Porphyromonas gingivalis, a gram-negative, black-pigmented anaerobe, is among the microorganisms implicated in the etiology of adult periodontal disease. This bacterium possesses a number of factors,...
Jespersgaard, Christina, Hajishengallis, George, Huang, Yan, Russell, Michael W., Smith, Daniel J., Michalek, Suzanne M.
Here we present the construction and characterization of a chimeric vaccine protein combining the glucan-binding domain (GLU) of the gtfB-encoded water-insoluble glucan-synthesizing...
Martin, Michael, Metzger, Daniel J., Michalek, Suzanne M., Connell, Terry D., Russell, Michael W.
Cholera toxin (CT) and the heat-labile enterotoxin of Escherichia coli (LT-I) are members of the serogroup I heat-labile enterotoxins (HLT) and can serve as systemic and mucosal adjuvants. However,...
Katz, Jannet, Sambandam, Vijaya, Wu, John H., Michalek, Suzanne M., Balkovetz, Daniel F.
Porphyromonas gingivalis is considered among the etiological agents of human adult periodontitis. Although in vitro studies have shown that P. gingivalis has the ability to invade epithelial cell...
Huang, Yan, Hajishengallis, George, Michalek, Suzanne M.
Attenuated Salmonella enterica serovar Typhimurium has been used for targeted delivery of recombinant antigens to the gut-associated lymphoid tissues. One potential problem associated with this...
Martin, Michael, Hajishengallis, George, Metzger, Daniel J., Michalek, Suzanne M., Connell, Terry D., Russell, Michael W.
The ADP-ribosylating enterotoxins, cholera toxin (CT) and the Escherichia coli heat-labile toxin (LT-IIa), have been shown to enhance mucosal and systemic antibody (Ab) responses to coadministered...
Hirschfeld, Matthew, Weis, Janis J., Toshchakov, Vladimir, Salkowski, Cindy A., Cody, M. Joshua, Ward, Dawn C., ...
Lipopolysaccharide (LPS) derived from the periodontal pathogen Porphyromonas gingivalis has been reported to differ structurally and functionally from enterobacterial LPS. These studies demonstrate...
Huang, Yan, Hajishengallis, George, Michalek, Suzanne M.
The purpose of the present study was to evaluate the effectiveness of an attenuated Salmonella enterica serovar Typhimurium vaccine strain expressing the saliva-binding region (SBR) of the...
Genetic Characterization of a Streptococcus mutans LraI Family Operon and Role in Virulence
Kitten, Todd, Munro, Cindy L., Michalek, Suzanne M., Macrina, Francis L.
Proteins belonging to the LraI (for “lipoprotein receptor antigen”) family function as adhesins in several streptococci, as a virulence factor for endocarditis in at least one of these species,...
Martin, Michael, Metzger, Daniel J., Michalek, Suzanne M., Connell, Terry D., Russell, Michael W.
Cholera toxin (CT) and the type II heat-labile enterotoxins (HLT) LT-IIa and LT-IIb act as potent systemic and mucosal adjuvants and induce distinct T-helper (Th)-cell cytokine profiles. In the...
Jespersgaard, Christina, Zhang, Ping, Hajishengallis, George, Russell, Michael W., Michalek, Suzanne M.
Attenuated Salmonella enterica serovar Typhimurium has been used for targeted delivery of recombinant antigens to gut- and nose-associated lymphoid tissues. Contradictory reports have described the...
Childers, Noel K., Miller, Keri L., Tong, Giang, Llarena, Juan Carlos, Greenway, Terrance, Ulrich, J. Terry, ...
The effectiveness of monophosphoryl lipid A (MPL) as a mucosal adjuvant was investigated following oral or intranasal (i.n.) administration of an aqueous adjuvant formulation of MPL (MPL-AF) added to...
Kent, Leigh W., Rahemtulla, Firoz, Hockett, Richard D., Gilleland, Rebecca C., Michalek, Suzanne M.
Cytokines are hormone-like proteins which mediate and regulate inflammatory and immune responses. The purpose of this study was to investigate the effect of lipopolysaccharide (LPS) and inflammatory...
Hajishengallis, George, Russell, Michael W., Michalek, Suzanne M.
Previous studies have identified an N-terminal saliva-binding region (SBR) on Streptococcus mutans surface antigen I/II (AgI/II) and suggested its importance in the initial adherence of S. mutans to...
Hazlett, Karsten R. O., Michalek, Suzanne M., Banas, Jeffrey A.
Glucan-binding protein A (GbpA) of Streptococcus mutans has been hypothesized to promote sucrose-dependent adherence and the cohesiveness of plaque and therefore to contribute to caries formation. We...
Harokopakis, Evlambia, Hajishengallis, George, Michalek, Suzanne M.
Liposomes appear to be a promising oral antigen delivery system for the development of vaccines against infectious diseases, although their uptake efficiency by Peyer’s patches in the gut and the...
Jespersgaard, Christina, Hajishengallis, George, Russell, Michael W., Michalek, Suzanne M.
Saliva contains an array of nonimmunoglobulin defense factors which are thought to contribute to the protection of the hard and soft tissue surfaces of the oral cavity by modulating microbial...
Hydrolysis of Epithelial Junctional Proteins by Porphyromonas gingivalis Gingipains
Katz, Jannet, Yang, Qiu-Bo, Zhang, Ping, Potempa, Jan, Travis, James, Michalek, Suzanne M., ...
Porphyromonas gingivalis has been implicated as an etiologic agent of adult periodontitis. We have previously shown that P. gingivalis can degrade the epithelial cell-cell junction complexes, thus...
Yang, Qiu-Bo, Martin, Michael, Michalek, Suzanne M., Katz, Jannet
Porphyromonas gingivalis, a gram-negative, black-pigmented anaerobe, is among the microorganisms implicated in the etiology of adult periodontal disease. This bacterium possesses a number of factors,...
Zhang, Ping, Jespersgaard, Christina, Lamberty-Mallory, Leticia, Katz, Jannet, Huang, Yan, Hajishengallis, George, ...
The saliva-binding region (SBR) of the cell surface antigen I/II (AgI/II) and the glucan-binding region (GLU) of the glucosyltransferase enzyme of Streptococcus mutans have been implicated in the...
Role of Innate Immune Factors in the Adjuvant Activity of Monophosphoryl Lipid A
Martin, Michael, Michalek, Suzanne M., Katz, Jannet
Monophosphoryl lipid A (MPL) is a nontoxic derivative of lipopolysaccharide (LPS) that exhibits adjuvant properties similar to those of the parent LPS molecule. However, the mechanism by which MPL...
Han, Seung Hyun, Kim, Je Hak, Martin, Michael, Michalek, Suzanne M., Nahm, Moon H.
Streptococcus pneumoniae is a leading cause of gram-positive sepsis, and lipoteichoic acid (LTA) may be important in causing gram-positive bacterial septic shock. Even though pneumococcal LTA is...
Zhang, Ping, Martin, Michael, Yang, Qiu-Bo, Michalek, Suzanne M., Katz, Jannet
In addition to antigen-specific signals mediated through the T-cell receptor, T cells also require antigen nonspecific costimulation for activation. The B7 family of molecules on antigen-presenting...
Kiyono, Hiroshi, McGhee, Jerry R., Wannemuehler, Michael J., Frangakis, Maria V., Spalding, David M., Michalek, Suzanne M., ...
The first line of defense against pathogens that enter the host by the oral route appears to involve the gut-associated lymphoreticular tissue—e.g., Peyer's patches (PP). Although animals can...
Garcia, Carlos A., Martin, Michael, Michalek, Suzanne M.
The purpose of the present study was to evaluate the ability of an attenuated Salmonella enterica serovar Typhimurium vaccine strain to up-regulate B7-1 and B7-2 on antigen-presenting cells and to...
Hirasawa, Masatomo, Kiyono, Hiroshi, Shiota, Tetsuo, Hull, Richard A., Curtiss, Roy, Michalek, Suzanne M., ...
Previous studies have shown that a mutant (designated C4) of Streptococcus mutans 6715 wild type (WT) is defective in glucosyltransferase (GTF)-synthesized insoluble glucan and is avirulent in...
Hirasawa, Masatoma, Kiyono, Hiroshi, Babb, James L., Shiota, Tetsuo, Michalek, Suzanne M., McGhee, Jerry R.
A mutant of Streptococcus mutans 6715 wild type (WT), designated C4, has been shown previously to be defective in glucosyltransferase synthesis of insoluble glucan and to have low virulence in...
Pino, Oscar, Martin, Michael, Michalek, Suzanne M.
An expanding area of interest is the utilization of microbe-based components to augment mucosal and systemic immune responses to target antigens. Thus, the aim of the present study was to assess if...
Han, Seung Hyun, Kim, Je Hak, Seo, Ho Seong, Martin, Michael H., Chung, Gook-Hyun, Michalek, Suzanne M., ...
NO production by macrophages in response to lipoteichoic acid (LTA) and a synthetic lipopeptide (Pam3CSK4) was investigated. LTA and Pam3CSK4 induced the production of both TNF-α and NO. Inhibitors...
Zhang, Ping, Martin, Michael, Michalek, Suzanne M., Katz, Jannet
Hemagglutinin B (HagB) is a nonfimbrial adhesin expressed on the surface of Porphyromonas gingivalis and has been implicated as a potential virulence factor involved in mediating the attachment of...
Toll-Like Receptor 2 Is Required for Inflammatory Responses to Francisella tularensis LVS
Katz, Jannet, Zhang, Ping, Martin, Michael, Vogel, Stefanie N., Michalek, Suzanne M.
Francisella tularensis, a gram-negative bacterium, is the etiologic agent of tularemia and has recently been classified as a category A bioterrorism agent. Infections with F. tularensis result in an...
Childers, Noel K., Tong, Giang, Mitchell, Stephen, Kirk, Katharine, Russell, Michael W., Michalek, Suzanne M.
Recent attention to mucosal immunization strategies has been focused on the nasal route for vaccine delivery. This study was designed to determine the effectiveness of a liposome-protein vaccine...
Jespersgaard, Christina, Hajishengallis, George, Greenway, Terrence E., Smith, Daniel J., Russell, Michael W., Michalek, Suzanne M.
Glucosyltransferase (GTF) enzymes of mutans streptococci are considered virulence factors due to their ability to synthesize adhesive glucans, which facilitate cell-to-cell adherence and...
Virulence of a spaP Mutant of Streptococcus mutans in a Gnotobiotic Rat Model
Crowley, Paula J., Brady, L. Jeannine, Michalek, Suzanne M., Bleiweis, Arnold S.
Streptococcus mutans, the principal etiologic agent of dental caries in humans, possesses a variety of virulence traits that enable it to establish itself in the oral cavity and initiate disease. A...
Katz, Jannet, Black, Kelley P., Michalek, Suzanne M.
Porphyromonas gingivalis, a gram-negative, black-pigmented anaerobe, is among the microorganisms implicated in the etiology of adult periodontal disease. This bacterium possesses a number of factors,...
Jespersgaard, Christina, Hajishengallis, George, Huang, Yan, Russell, Michael W., Smith, Daniel J., Michalek, Suzanne M.
Here we present the construction and characterization of a chimeric vaccine protein combining the glucan-binding domain (GLU) of the gtfB-encoded water-insoluble glucan-synthesizing...
Martin, Michael, Metzger, Daniel J., Michalek, Suzanne M., Connell, Terry D., Russell, Michael W.
Cholera toxin (CT) and the heat-labile enterotoxin of Escherichia coli (LT-I) are members of the serogroup I heat-labile enterotoxins (HLT) and can serve as systemic and mucosal adjuvants. However,...
Katz, Jannet, Sambandam, Vijaya, Wu, John H., Michalek, Suzanne M., Balkovetz, Daniel F.
Porphyromonas gingivalis is considered among the etiological agents of human adult periodontitis. Although in vitro studies have shown that P. gingivalis has the ability to invade epithelial cell...
Huang, Yan, Hajishengallis, George, Michalek, Suzanne M.
Attenuated Salmonella enterica serovar Typhimurium has been used for targeted delivery of recombinant antigens to the gut-associated lymphoid tissues. One potential problem associated with this...
Martin, Michael, Hajishengallis, George, Metzger, Daniel J., Michalek, Suzanne M., Connell, Terry D., Russell, Michael W.
The ADP-ribosylating enterotoxins, cholera toxin (CT) and the Escherichia coli heat-labile toxin (LT-IIa), have been shown to enhance mucosal and systemic antibody (Ab) responses to coadministered...
Hirschfeld, Matthew, Weis, Janis J., Toshchakov, Vladimir, Salkowski, Cindy A., Cody, M. Joshua, Ward, Dawn C., ...
Lipopolysaccharide (LPS) derived from the periodontal pathogen Porphyromonas gingivalis has been reported to differ structurally and functionally from enterobacterial LPS. These studies demonstrate...
Huang, Yan, Hajishengallis, George, Michalek, Suzanne M.
The purpose of the present study was to evaluate the effectiveness of an attenuated Salmonella enterica serovar Typhimurium vaccine strain expressing the saliva-binding region (SBR) of the...
Genetic Characterization of a Streptococcus mutans LraI Family Operon and Role in Virulence
Kitten, Todd, Munro, Cindy L., Michalek, Suzanne M., Macrina, Francis L.
Proteins belonging to the LraI (for “lipoprotein receptor antigen”) family function as adhesins in several streptococci, as a virulence factor for endocarditis in at least one of these species,...
Martin, Michael, Metzger, Daniel J., Michalek, Suzanne M., Connell, Terry D., Russell, Michael W.
Cholera toxin (CT) and the type II heat-labile enterotoxins (HLT) LT-IIa and LT-IIb act as potent systemic and mucosal adjuvants and induce distinct T-helper (Th)-cell cytokine profiles. In the...
Jespersgaard, Christina, Zhang, Ping, Hajishengallis, George, Russell, Michael W., Michalek, Suzanne M.
Attenuated Salmonella enterica serovar Typhimurium has been used for targeted delivery of recombinant antigens to gut- and nose-associated lymphoid tissues. Contradictory reports have described the...
Childers, Noel K., Miller, Keri L., Tong, Giang, Llarena, Juan Carlos, Greenway, Terrance, Ulrich, J. Terry, ...
The effectiveness of monophosphoryl lipid A (MPL) as a mucosal adjuvant was investigated following oral or intranasal (i.n.) administration of an aqueous adjuvant formulation of MPL (MPL-AF) added to...
Kent, Leigh W., Rahemtulla, Firoz, Hockett, Richard D., Gilleland, Rebecca C., Michalek, Suzanne M.
Cytokines are hormone-like proteins which mediate and regulate inflammatory and immune responses. The purpose of this study was to investigate the effect of lipopolysaccharide (LPS) and inflammatory...
Hajishengallis, George, Russell, Michael W., Michalek, Suzanne M.
Previous studies have identified an N-terminal saliva-binding region (SBR) on Streptococcus mutans surface antigen I/II (AgI/II) and suggested its importance in the initial adherence of S. mutans to...
Hazlett, Karsten R. O., Michalek, Suzanne M., Banas, Jeffrey A.
Glucan-binding protein A (GbpA) of Streptococcus mutans has been hypothesized to promote sucrose-dependent adherence and the cohesiveness of plaque and therefore to contribute to caries formation. We...
Harokopakis, Evlambia, Hajishengallis, George, Michalek, Suzanne M.
Liposomes appear to be a promising oral antigen delivery system for the development of vaccines against infectious diseases, although their uptake efficiency by Peyer’s patches in the gut and the...
Jespersgaard, Christina, Hajishengallis, George, Russell, Michael W., Michalek, Suzanne M.
Saliva contains an array of nonimmunoglobulin defense factors which are thought to contribute to the protection of the hard and soft tissue surfaces of the oral cavity by modulating microbial...
Hydrolysis of Epithelial Junctional Proteins by Porphyromonas gingivalis Gingipains
Katz, Jannet, Yang, Qiu-Bo, Zhang, Ping, Potempa, Jan, Travis, James, Michalek, Suzanne M., ...
Porphyromonas gingivalis has been implicated as an etiologic agent of adult periodontitis. We have previously shown that P. gingivalis can degrade the epithelial cell-cell junction complexes, thus...
Yang, Qiu-Bo, Martin, Michael, Michalek, Suzanne M., Katz, Jannet
Porphyromonas gingivalis, a gram-negative, black-pigmented anaerobe, is among the microorganisms implicated in the etiology of adult periodontal disease. This bacterium possesses a number of factors,...
Zhang, Ping, Jespersgaard, Christina, Lamberty-Mallory, Leticia, Katz, Jannet, Huang, Yan, Hajishengallis, George, ...
The saliva-binding region (SBR) of the cell surface antigen I/II (AgI/II) and the glucan-binding region (GLU) of the glucosyltransferase enzyme of Streptococcus mutans have been implicated in the...
Role of Innate Immune Factors in the Adjuvant Activity of Monophosphoryl Lipid A
Martin, Michael, Michalek, Suzanne M., Katz, Jannet
Monophosphoryl lipid A (MPL) is a nontoxic derivative of lipopolysaccharide (LPS) that exhibits adjuvant properties similar to those of the parent LPS molecule. However, the mechanism by which MPL...
Han, Seung Hyun, Kim, Je Hak, Martin, Michael, Michalek, Suzanne M., Nahm, Moon H.
Streptococcus pneumoniae is a leading cause of gram-positive sepsis, and lipoteichoic acid (LTA) may be important in causing gram-positive bacterial septic shock. Even though pneumococcal LTA is...
Zhang, Ping, Martin, Michael, Yang, Qiu-Bo, Michalek, Suzanne M., Katz, Jannet
In addition to antigen-specific signals mediated through the T-cell receptor, T cells also require antigen nonspecific costimulation for activation. The B7 family of molecules on antigen-presenting...
Kiyono, Hiroshi, McGhee, Jerry R., Wannemuehler, Michael J., Frangakis, Maria V., Spalding, David M., Michalek, Suzanne M., ...
The first line of defense against pathogens that enter the host by the oral route appears to involve the gut-associated lymphoreticular tissue—e.g., Peyer's patches (PP). Although animals can...
Garcia, Carlos A., Martin, Michael, Michalek, Suzanne M.
The purpose of the present study was to evaluate the ability of an attenuated Salmonella enterica serovar Typhimurium vaccine strain to up-regulate B7-1 and B7-2 on antigen-presenting cells and to...