Sylvain Brisse

Genome Microevolution of Chikungunya Viruses Causing the Indian Ocean Outbreak (2006)

Isabelle Schuffenecker, Isabelle Iteman, Alain Michault, Séverine Murri, Lionel Frangeul, Marie-Christine Vaney, ...

Viral genome sequence isolated from 6 patients and partial sequences of isolates from 121 patients at different stages and locations of the outbreak reveals unique and evolving genetic features.

Genome Microevolution of Chikungunya Viruses Causing the Indian Ocean Outbreak (2006)

Isabelle Schuffenecker, Isabelle Iteman, Alain Michault, Séverine Murri, Lionel Frangeul, Marie-Christine Vaney, ...

BackgroundA chikungunya virus outbreak of unprecedented magnitude is currently ongoing in Indian Ocean territories. In Réunion Island, this alphavirus has already infected about one-third of the...

Ancient origin and gene mosaicism of the progenitor of Mycobacterium tuberculosis. (2006)

Gutierrez, M Cristina, Brisse, Sylvain, Brosch, Roland, Fabre, Michel, Omaïs, Bahia, Marmiesse, Magali, ...

The highly successful human pathogen Mycobacterium tuberculosis has an extremely low level of genetic variation, which suggests that the entire population resulted from clonal expansion following an...

Ancient origin and gene mosaicism of the progenitor of Mycobacterium tuberculosis. (2006)

Gutierrez, M Cristina, Brisse, Sylvain, Brosch, Roland, Fabre, Michel, Omaïs, Bahia, Marmiesse, Magali, ...

The highly successful human pathogen Mycobacterium tuberculosis has an extremely low level of genetic variation, which suggests that the entire population resulted from clonal expansion following an...

Ancient Origin and Gene Mosaicism of the Progenitor of Mycobacterium tuberculosis (2005)

M. Cristina Gutierrez, Sylvain Brisse, Roland Brosch, Michel Fabre, Bahia Omaïs, Magali Marmiesse, ...

The highly successful human pathogen Mycobacterium tuberculosis has an extremely low level of genetic variation, which suggests that the entire population resulted from clonal expansion following an...

Ancient origin and gene mosaicism of the progenitor of Mycobacterium tuberculosis. (2005)

Gutierrez, M Cristina, Brisse, Sylvain, Brosch, Roland, Fabre, Michel, Omaïs, Bahia, Marmiesse, Magali, ...

The highly successful human pathogen Mycobacterium tuberculosis has an extremely low level of genetic variation, which suggests that the entire population resulted from clonal expansion following an...

Distinguishing Species of the Burkholderia cepacia Complex and Burkholderia gladioli by Automated Ribotyping

Brisse, Sylvain, Verduin, Cees M., Milatovic, Dana, Fluit, Ad, Verhoef, Jan, Laevens, Severine, ...

Several species belonging to the genus Burkholderia are clinically relevant, opportunistic pathogens that inhabit major environmental reservoirs. Consequently, the availability of means for adequate...

Molecular Surveillance of European Quinolone-Resistant Clinical Isolates of Pseudomonas aeruginosa and Acinetobacter spp. Using Automated Ribotyping

Brisse, Sylvain, Milatovic, Dana, Fluit, A. C., Kusters, Karlijn, Toelstra, Annet, Verhoef, Jan, ...

Nosocomial isolates of Pseudomonas aeruginosa and Acinetobacter spp. exhibit high rates of resistance to antibiotics and are often multidrug resistant. In a previous study (D. Milatovic, A. Fluit, S....

Association of Alterations in ParC and GyrA Proteins with Resistance of Clinical Isolates of Enterococcus faecium to Nine Different Fluoroquinolones

Brisse, Sylvain, Fluit, Ad C., Wagner, Ulrich, Heisig, Peter, Milatovic, Dana, Verhoef, Jan, ...

The parC and gyrA genes of 73 ciprofloxacin-resistant and 6 ciprofloxacin-susceptible Enterococcus faecium clinical isolates were partly sequenced. Alterations in ParC and GyrA, possibly in...

In Vitro Activities of Sitafloxacin (DU-6859a) and Six Other Fluoroquinolones against 8,796 Clinical Bacterial Isolates

Milatovic, Dana, Schmitz, Franz-Josef, Brisse, Sylvain, Verhoef, Jan, Fluit, Ad C.

The in vitro activities of sitafloxacin, ciprofloxacin, trovafloxacin, levofloxacin, clinafloxacin, gatifloxacin, and moxifloxacin against 5,046 gram-negative bacteria, 3,344 gram-positive cocci, and...

Automated Ribotyping of Vancomycin-Resistant Enterococcus faecium Isolates

Brisse, Sylvain, Fussing, Vivian, Ridwan, Ben, Verhoef, Jan, Willems, Rob J. L.

Vancomycin-resistant Enterococcus faecium (VREF) strains represent an important threat in hospital infections in the United States and are found at high frequencies in both the community and farm...

Evaluation of the Etest ESBL and the BD Phoenix, VITEK 1, and VITEK 2 Automated Instruments for Detection of Extended-Spectrum Beta-Lactamases in Multiresistant Escherichia coli and Klebsiella spp.

Fluit, Ad C., Paauw, Armand, Box, Adrienne T. A., Brisse, Sylvain, Verhoef, Jan

Seventy-four isolates of multiresistant Escherichia coli and Klebsiella spp. recovered during a 3-year period and 17 control strains with genotypically identified beta-lactamases were tested for the...

Comparative Evaluation of the BD Phoenix and VITEK 2 Automated Instruments for Identification of Isolates of the Burkholderia cepacia Complex

Brisse, Sylvain, Stefani, Stefania, Verhoef, Jan, Van Belkum, Alex, Vandamme, Peter, Goessens, Wil

We evaluated two new automated identification systems, the BD Phoenix (Becton Dickinson) and the VITEK 2 (bioMérieux), for identification of isolates of the Burkholderia cepacia complex (BCC). The...

Evaluation of the Polymorphisms Associated with Tandem Repeats for Pseudomonas aeruginosa Strain Typing

Onteniente, Lucie, Brisse, Sylvain, Tassios, Panayotis T., Vergnaud, Gilles

We report on the development of a scheme for the typing of Pseudomonas aeruginosa, multiple-locus variable number of tandem repeat (VNTR) analysis (MLVA). We first evaluated the polymorphisms of 201...

Molecular Serotyping of Klebsiella Species Isolates by Restriction of the Amplified Capsular Antigen Gene Cluster

Brisse, Sylvain, Issenhuth-Jeanjean, Sylvie, Grimont, Patrick A. D.

The objective of the present work was to develop a molecular method that would enable determination of the capsular serotypes of Klebsiella isolates without the use of antiserum. PCR amplification of...

Species Distribution and Ribotype Diversity of Burkholderia cepacia Complex Isolates from French Patients with Cystic Fibrosis

Brisse, Sylvain, Cordevant, Christophe, Vandamme, Peter, Bidet, Philippe, Loukil, Chawki, Chabanon, Gérard, ...

A total of 153 Burkholderia cepacia strains obtained from 153 French patients with cystic fibrosis were identified as Burkholderia multivorans (51.6%) or Burkholderia cenocepacia (45.1%). Eighty-two...

Six Groups of the OXY β-Lactamase Evolved over Millions of Years in Klebsiella oxytoca

Fevre, Cindy, Jbel, Mehdi, Passet, Virginie, Weill, François-Xavier, Grimont, Patrick A. D., Brisse, Sylvain

The diversity and evolution of the class A OXY β-lactamase from Klebsiella oxytoca were investigated and compared to housekeeping gene diversity. The entire blaOXY coding region was sequenced in 18...

Multilocus Sequence Typing of Klebsiella pneumoniae Nosocomial Isolates

Diancourt, Laure, Passet, Virginie, Verhoef, Jan, Grimont, Patrick A. D., Brisse, Sylvain

A multilocus sequence typing (MLST) scheme was developed for Klebsiella pneumoniae. Sequences of seven housekeeping genes were obtained for 67 K. pneumoniae strains, including 19 ceftazidime- and...

Regional Occurrence of Plasmid-Mediated Carbapenem-Hydrolyzing Oxacillinase OXA-58 in Acinetobacter spp. in Europe

Marqué, Sophie, Poirel, Laurent, Héritier, Claire, Brisse, Sylvain, Blasco, Maria Dolores, Filip, Roxana, ...

The spread of the plasmid-mediated carbapenem-hydrolyzing oxacillinase OXA-58 was detected in Acinetobacter sp. clinical isolates from southern Europe, the Balkans, and central Turkey. It may...

Ancient Origin and Gene Mosaicism of the Progenitor of Mycobacterium tuberculosis

Gutierrez, M. Cristina, Brisse, Sylvain, Brosch, Roland, Fabre, Michel, Omaïs, Bahia, Marmiesse, Magali, ...

The highly successful human pathogen Mycobacterium tuberculosis has an extremely low level of genetic variation, which suggests that the entire population resulted from clonal expansion following an...

Variants of the Klebsiella pneumoniae OKP Chromosomal Beta-Lactamase Are Divided into Two Main Groups, OKP-A and OKP-B

Fevre, Cindy, Passet, Virginie, Weill, François-Xavier, Grimont, Patrick A. D., Brisse, Sylvain

Two blaOKP subgroups were found, diverging by 4.2%. Subgroups blaOKP-A (10 enzyme variants, pIs from 7.1 to 8.3) and blaOKP-B (11 variants, pI 7.1) showed similar antibiotic susceptibilities....

Genome Microevolution of Chikungunya Viruses Causing the Indian Ocean Outbreak

Schuffenecker, Isabelle, Iteman, Isabelle, Michault, Alain, Murri, Séverine, Frangeul, Lionel, Vaney, Marie-Christine, ...

Viral genome sequence isolated from 6 patients and partial sequences of isolates from 121 patients at different stages and locations of the outbreak reveals unique and evolving genetic features.

Identification of Acinetobacter Species and Genotyping of Acinetobacter baumannii by Multilocus PCR and Mass Spectrometry

Ecker, Joseph A., Massire, Christian, Hall, Thomas A., Ranken, Raymond, Pennella, Thuy-Trang D., Agasino Ivy, Cristina, ...

Members of the genus Acinetobacter are ubiquitous in soil and water and are an important cause of nosocomial infections. A rapid method is needed to genotype Acinetobacter isolates to determine...

Distinguishing Species of the Burkholderia cepacia Complex and Burkholderia gladioli by Automated Ribotyping

Brisse, Sylvain, Verduin, Cees M., Milatovic, Dana, Fluit, Ad, Verhoef, Jan, Laevens, Severine, ...

Several species belonging to the genus Burkholderia are clinically relevant, opportunistic pathogens that inhabit major environmental reservoirs. Consequently, the availability of means for adequate...

Molecular Surveillance of European Quinolone-Resistant Clinical Isolates of Pseudomonas aeruginosa and Acinetobacter spp. Using Automated Ribotyping

Brisse, Sylvain, Milatovic, Dana, Fluit, A. C., Kusters, Karlijn, Toelstra, Annet, Verhoef, Jan, ...

Nosocomial isolates of Pseudomonas aeruginosa and Acinetobacter spp. exhibit high rates of resistance to antibiotics and are often multidrug resistant. In a previous study (D. Milatovic, A. Fluit, S....

Association of Alterations in ParC and GyrA Proteins with Resistance of Clinical Isolates of Enterococcus faecium to Nine Different Fluoroquinolones

Brisse, Sylvain, Fluit, Ad C., Wagner, Ulrich, Heisig, Peter, Milatovic, Dana, Verhoef, Jan, ...

The parC and gyrA genes of 73 ciprofloxacin-resistant and 6 ciprofloxacin-susceptible Enterococcus faecium clinical isolates were partly sequenced. Alterations in ParC and GyrA, possibly in...

In Vitro Activities of Sitafloxacin (DU-6859a) and Six Other Fluoroquinolones against 8,796 Clinical Bacterial Isolates

Milatovic, Dana, Schmitz, Franz-Josef, Brisse, Sylvain, Verhoef, Jan, Fluit, Ad C.

The in vitro activities of sitafloxacin, ciprofloxacin, trovafloxacin, levofloxacin, clinafloxacin, gatifloxacin, and moxifloxacin against 5,046 gram-negative bacteria, 3,344 gram-positive cocci, and...

Automated Ribotyping of Vancomycin-Resistant Enterococcus faecium Isolates

Brisse, Sylvain, Fussing, Vivian, Ridwan, Ben, Verhoef, Jan, Willems, Rob J. L.

Vancomycin-resistant Enterococcus faecium (VREF) strains represent an important threat in hospital infections in the United States and are found at high frequencies in both the community and farm...

Evaluation of the Etest ESBL and the BD Phoenix, VITEK 1, and VITEK 2 Automated Instruments for Detection of Extended-Spectrum Beta-Lactamases in Multiresistant Escherichia coli and Klebsiella spp.

Fluit, Ad C., Paauw, Armand, Box, Adrienne T. A., Brisse, Sylvain, Verhoef, Jan

Seventy-four isolates of multiresistant Escherichia coli and Klebsiella spp. recovered during a 3-year period and 17 control strains with genotypically identified beta-lactamases were tested for the...

Comparative Evaluation of the BD Phoenix and VITEK 2 Automated Instruments for Identification of Isolates of the Burkholderia cepacia Complex

Brisse, Sylvain, Stefani, Stefania, Verhoef, Jan, Van Belkum, Alex, Vandamme, Peter, Goessens, Wil

We evaluated two new automated identification systems, the BD Phoenix (Becton Dickinson) and the VITEK 2 (bioMérieux), for identification of isolates of the Burkholderia cepacia complex (BCC). The...

Evaluation of the Polymorphisms Associated with Tandem Repeats for Pseudomonas aeruginosa Strain Typing

Onteniente, Lucie, Brisse, Sylvain, Tassios, Panayotis T., Vergnaud, Gilles

We report on the development of a scheme for the typing of Pseudomonas aeruginosa, multiple-locus variable number of tandem repeat (VNTR) analysis (MLVA). We first evaluated the polymorphisms of 201...

Molecular Serotyping of Klebsiella Species Isolates by Restriction of the Amplified Capsular Antigen Gene Cluster

Brisse, Sylvain, Issenhuth-Jeanjean, Sylvie, Grimont, Patrick A. D.

The objective of the present work was to develop a molecular method that would enable determination of the capsular serotypes of Klebsiella isolates without the use of antiserum. PCR amplification of...

Species Distribution and Ribotype Diversity of Burkholderia cepacia Complex Isolates from French Patients with Cystic Fibrosis

Brisse, Sylvain, Cordevant, Christophe, Vandamme, Peter, Bidet, Philippe, Loukil, Chawki, Chabanon, Gérard, ...

A total of 153 Burkholderia cepacia strains obtained from 153 French patients with cystic fibrosis were identified as Burkholderia multivorans (51.6%) or Burkholderia cenocepacia (45.1%). Eighty-two...

Six Groups of the OXY β-Lactamase Evolved over Millions of Years in Klebsiella oxytoca

Fevre, Cindy, Jbel, Mehdi, Passet, Virginie, Weill, François-Xavier, Grimont, Patrick A. D., Brisse, Sylvain

The diversity and evolution of the class A OXY β-lactamase from Klebsiella oxytoca were investigated and compared to housekeeping gene diversity. The entire blaOXY coding region was sequenced in 18...

Multilocus Sequence Typing of Klebsiella pneumoniae Nosocomial Isolates

Diancourt, Laure, Passet, Virginie, Verhoef, Jan, Grimont, Patrick A. D., Brisse, Sylvain

A multilocus sequence typing (MLST) scheme was developed for Klebsiella pneumoniae. Sequences of seven housekeeping genes were obtained for 67 K. pneumoniae strains, including 19 ceftazidime- and...

Regional Occurrence of Plasmid-Mediated Carbapenem-Hydrolyzing Oxacillinase OXA-58 in Acinetobacter spp. in Europe

Marqué, Sophie, Poirel, Laurent, Héritier, Claire, Brisse, Sylvain, Blasco, Maria Dolores, Filip, Roxana, ...

The spread of the plasmid-mediated carbapenem-hydrolyzing oxacillinase OXA-58 was detected in Acinetobacter sp. clinical isolates from southern Europe, the Balkans, and central Turkey. It may...

Ancient Origin and Gene Mosaicism of the Progenitor of Mycobacterium tuberculosis

Gutierrez, M. Cristina, Brisse, Sylvain, Brosch, Roland, Fabre, Michel, Omaïs, Bahia, Marmiesse, Magali, ...

The highly successful human pathogen Mycobacterium tuberculosis has an extremely low level of genetic variation, which suggests that the entire population resulted from clonal expansion following an...

Variants of the Klebsiella pneumoniae OKP Chromosomal Beta-Lactamase Are Divided into Two Main Groups, OKP-A and OKP-B

Fevre, Cindy, Passet, Virginie, Weill, François-Xavier, Grimont, Patrick A. D., Brisse, Sylvain

Two blaOKP subgroups were found, diverging by 4.2%. Subgroups blaOKP-A (10 enzyme variants, pIs from 7.1 to 8.3) and blaOKP-B (11 variants, pI 7.1) showed similar antibiotic susceptibilities....

Genome Microevolution of Chikungunya Viruses Causing the Indian Ocean Outbreak

Schuffenecker, Isabelle, Iteman, Isabelle, Michault, Alain, Murri, Séverine, Frangeul, Lionel, Vaney, Marie-Christine, ...

Viral genome sequence isolated from 6 patients and partial sequences of isolates from 121 patients at different stages and locations of the outbreak reveals unique and evolving genetic features.

Identification of Acinetobacter Species and Genotyping of Acinetobacter baumannii by Multilocus PCR and Mass Spectrometry

Ecker, Joseph A., Massire, Christian, Hall, Thomas A., Ranken, Raymond, Pennella, Thuy-Trang D., Agasino Ivy, Cristina, ...

Members of the genus Acinetobacter are ubiquitous in soil and water and are an important cause of nosocomial infections. A rapid method is needed to genotype Acinetobacter isolates to determine...

Multilocus Sequence Typing of Lactobacillus casei Reveals a Clonal Population Structure with Low Levels of Homologous Recombination▿ †

Diancourt, Laure, Passet, Virginie, Chervaux, Christian, Garault, Peggy, Smokvina, Tamara, Brisse, Sylvain

Robust genotyping methods for Lactobacillus casei are needed for strain tracking and collection management, as well as for population biology research. A collection of 52 strains initially labeled L....

Recombining Population Structure of Plesiomonas shigelloides (Enterobacteriaceae) Revealed by Multilocus Sequence Typing▿ †

Salerno, Anna, Delétoile, Alexis, Lefevre, Martine, Ciznar, Ivan, Krovacek, Karel, Grimont, Patrick, ...

Plesiomonas shigelloides is an emerging pathogen that is widespread in the aquatic environment and is responsible for intestinal diseases and extraintestinal infections in humans and other animals....

A New Perspective on Listeria monocytogenes Evolution

Ragon, Marie, Wirth, Thierry, Hollandt, Florian, Lavenir, Rachel, Lecuit, Marc, Le Monnier, Alban, ...

Listeria monocytogenes is a model organism for cellular microbiology and host–pathogen interaction studies and an important food-borne pathogen widespread in the environment, thus representing an...

Origin, Spread and Demography of the Mycobacterium tuberculosis Complex

Wirth, Thierry, Hildebrand, Falk, Allix-Béguec, Caroline, Wölbeling, Florian, Kubica, Tanja, Kremer, Kristin, ...

The evolutionary timing and spread of the Mycobacterium tuberculosis complex (MTBC), one of the most successful groups of bacterial pathogens, remains largely unknown. Here, using mycobacterial...