T. A. Berkhout

Static and time-resolved fluorescence studies of the fluorescent phosphatidylcholing bound to the phosphatidylcholine transfer protein of bovine liver (1984)

Berkhout, T.A., Wirtz, K.W.A.

Identification of membrane proteins of human blood platelets with a hydrophobic photolabel (1984)

Berkhout, T.A., Schiphorst, M.E., Wirtz, K.W.A., Sixma, J.J.

A photoactivable glycolipid probe, 12-(4-azido-2-nitrophenoxy)stearoyl[1-14C]glucosamine, was used to label proteins and lipids of platelet membranes. The proteins were analyzed by two-dimensional...

The effect of (-)-hydroxycitrate on the activity of the low-density-lipoprotein receptor and 3-hydroxy-3-methylglutaryl-CoA reductase levels in the human hepatoma cell line Hep G2.

Berkhout, T A, Havekes, L M, Pearce, N J, Groot, P H

(-)-Hydroxycitrate, a potent inhibitor of ATP citrate-lyase, was tested in Hep G2 cells for effects on cholesterol homoeostasis. After 2.5 h and 18 h incubations with (-)-hydroxycitrate at...

Analysis of histones from the yeast Saccharomyces carlsbergensis.

Pastink, A, Berkhout, T A, Mager, W H, Planta, R J

Basic chromosomal proteins were isolated from the chromatin of the yeast Saccharomyces carlsbergensis by extraction with H2SO4 and were purified by ion-exchange chromatography. Electrophoresis of the...

The role of ATP citrate-lyase in the metabolic regulation of plasma lipids. Hypolipidaemic effects of SB-204990, a lactone prodrug of the potent ATP citrate-lyase inhibitor SB-201076.

Pearce, N J, Yates, J W, Berkhout, T A, Jackson, B, Tew, D, Boyd, H, ...

ATP citrate (pro-S)-lyase (EC 4.1.3.8), a cytosolic enzyme that generates acetyl-CoA for cholesterol and fatty acid synthesis de novo, is a potential target for hypolipidaemic intervention. Here we...

The effect of (-)-hydroxycitrate on the activity of the low-density-lipoprotein receptor and 3-hydroxy-3-methylglutaryl-CoA reductase levels in the human hepatoma cell line Hep G2.

Berkhout, T A, Havekes, L M, Pearce, N J, Groot, P H

(-)-Hydroxycitrate, a potent inhibitor of ATP citrate-lyase, was tested in Hep G2 cells for effects on cholesterol homoeostasis. After 2.5 h and 18 h incubations with (-)-hydroxycitrate at...

Analysis of histones from the yeast Saccharomyces carlsbergensis.

Pastink, A, Berkhout, T A, Mager, W H, Planta, R J

Basic chromosomal proteins were isolated from the chromatin of the yeast Saccharomyces carlsbergensis by extraction with H2SO4 and were purified by ion-exchange chromatography. Electrophoresis of the...

The role of ATP citrate-lyase in the metabolic regulation of plasma lipids. Hypolipidaemic effects of SB-204990, a lactone prodrug of the potent ATP citrate-lyase inhibitor SB-201076.

Pearce, N J, Yates, J W, Berkhout, T A, Jackson, B, Tew, D, Boyd, H, ...

ATP citrate (pro-S)-lyase (EC 4.1.3.8), a cytosolic enzyme that generates acetyl-CoA for cholesterol and fatty acid synthesis de novo, is a potential target for hypolipidaemic intervention. Here we...