Koppers-Lalic, D., Reits, E.A., Ressing, M.E., Lipinska, A.D., Abele, R., Koch, J., ...
Detection and elimination of virus-infected cells by cytotoxic T lymphocytes depends on recognition of virus-derived peptides presented by MHC class I molecules. A critical step in this process is...
Cell surface proteoglycans are not essential for infection by pseudorabies virus.
Karger, A, Saalmüller, A, Tufaro, F, Banfield, B W, Mettenleiter, T C
Cell surface proteoglycans, in particular those carrying heparan sulfate glycosaminoglycans, play a major role in primary attachment of herpesviruses to target cells. In pseudorabies virus (PrV),...
Baumeister, J, Klupp, B G, Mettenleiter, T C
We have determined the nucleotide sequence and transcriptional pattern of a group of open reading frames in the pseudorabies virus (PrV) genome located near the left end of the unique long region...
Jöns, A, Granzow, H, Kuchling, R, Mettenleiter, T C
Sequence analysis of BamHI fragment 1 of the pseudorabies virus (PrV) genome identified a novel PrV gene located upstream of the UL50 gene encoding PrV dUTPase. The deduced protein product displayed...
Identification and characterization of pseudorabies virus dUTPase.
Sequence analysis within the long segment of the pseudorabies virus (PrV) genome identified an open reading frame of 804 bp whose deduced protein product of 268 amino acids exhibited homology to...
Identification of cell surface molecules that interact with pseudorabies virus.
The alphaherpesvirus pseudorabies virus (PrV) has been shown to attach to cells by interaction between the viral glycoprotein gC and cell membrane proteoglycans carrying heparan sulfate chains...
Fuchs, W, Klupp, B G, Granzow, H, Rziha, H J, Mettenleiter, T C
Alphaherpesvirus genomes exhibit a generally collinear gene arrangement, and most of their genes are conserved among the different members of the subfamily. Among the exceptions is the UL3.5 gene of...
Dijkstra, J M, Visser, N, Mettenleiter, T C, Klupp, B G
Sequence analysis within BamHI fragment 3 of the pseudorabies virus (PrV) genome revealed an open reading frame homologous to the UL10 gene of herpes simplex virus. A rabbit antiserum directed...
Adaptability in herpesviruses: glycoprotein D-independent infectivity of pseudorabies virus.
Schmidt, J, Klupp, B G, Karger, A, Mettenleiter, T C
Initial contact between herpesviruses and host cells is mediated by virion envelope glycoproteins which bind to cellular receptors. In several alphaherpesviruses, the nonessential glycoprotein gC has...
Granzow, H, Weiland, F, Jöns, A, Klupp, B G, Karger, A, Mettenleiter, T C
We reinvestigated major steps in the replicative cycle of pseudorabies virus (PrV) by electron microscopy of infected cultured cells. Virions attached to the cell surface were found in two distinct...
Klupp, B G, Karger, A, Mettenleiter, T C
Attachment to cell surface heparan sulfate proteoglycans is the first step in infection by several alphaherpesviruses. This interaction is primarily mediated by virion glycoprotein C (gC). In herpes...
The pseudorabies virus UL51 gene product is a 30-kilodalton virion component.
Lenk, M, Visser, N, Mettenleiter, T C
Positional homologs to the UL51 open reading frame of herpes simplex virus type 1 have been identified throughout the herpesvirus family. However, no respective protein has so far been described for...
The UL20 gene product of pseudorabies virus functions in virus egress.
Fuchs, W, Klupp, B G, Granzow, H, Mettenleiter, T C
The UL20 open reading frame is positionally conserved in different alphaherpesvirus genomes and is predicted to encode an integral membrane protein. A previously described UL20- mutant of herpes...
Klupp, B G, Fuchs, W, Weiland, E, Mettenleiter, T C
Herpesviruses contain a number of envelope glycoproteins which play important roles in the interaction between virions and target cells. Although several glycoproteins are not present in all...
Favoreel, H W, Nauwynck, H J, Van Oostveldt, P, Mettenleiter, T C, Pensaert, M B
Fluorescein isothiocyanate-labeled porcine pseudorabies virus (PrV) polyclonal antibodies were added to PrV-infected swine kidney cells in vitro at 37 degrees C. In approximately 47% of the infected...
A second form of infectious bursal disease virus-associated tubule contains VP4.
Granzow, H, Birghan, C, Mettenleiter, T C, Beyer, J, Köllner, B, Mundt, E
Preparations of density gradient-purified infectious bursal disease virus (IBDV) were found to contain full and empty icosahedral virions, type I tubules with a diameter of about 60 nm, and type II...
Fuchs, W, Granzow, H, Mettenleiter, T C
The UL3.5 gene is positionally conserved but highly variable in size and sequence in different members of the Alphaherpesvirinae and is absent from herpes simplex virus genomes. We have shown...
Glycoproteins homologous to gB of herpes simplex virus (HSV) constitute the most highly conserved family of herpesvirus glycoproteins. All gB homologs analyzed so far have been shown to play...
Kopp, A, Blewett, E, Misra, V, Mettenleiter, T C
Glycoprotein B homologs represent the most highly conserved group of herpesvirus glycoproteins. They exist in oligomeric forms based on a dimeric structure. Despite the high degree of sequence and...
Identification and characterization of a novel structural glycoprotein in pseudorabies virus, gL.
Klupp, B G, Baumeister, J, Karger, A, Visser, N, Mettenleiter, T C
Herpesvirus envelope glycoproteins play important roles in the interaction between virions and target cells. In the alphaherpesvirus pseudorabies virus (PrV), seven glycoproteins that all constitute...
Mettenleiter, T C, Saalmüller, A, Weiland, F
In pseudorabies virus (PrV), an open reading frame that partially overlaps the gene for the essential glycoprotein gII has been shown to encode a protein homologous to the ICP18.5 polypeptide of...
Heffner, S, Kovács, F, Klupp, B G, Mettenleiter, T C
Essential herpesvirus glycoproteins are involved in membrane fusion processes during infection, e.g., viral penetration and direct cell-to-cell transmission. We previously showed that the...
Babic, N, Mettenleiter, T C, Flamand, A, Ugolini, G
The propagation of pseudorabies virus (PrV) mutants deficient in essential glycoproteins gp50 and gII was studied after inoculation of transcomplemented gp50- and gII- PrV into the motor hypoglossal...
Rauh, I, Weiland, F, Fehler, F, Keil, G M, Mettenleiter, T C
The genome of pseudorabies virus (PrV) encodes at least seven glycoproteins. The glycoprotein complex gII consists of three related polypeptides, two of them derived by proteolytic cleavage from a...
Fehler, F, Herrmann, J M, Saalmüller, A, Mettenleiter, T C, Keil, G M
Glycoprotein IV (gIV) of bovine herpesvirus 1 (BHV-1), a homolog of herpes simplex virus glycoprotein D, represents a major component of the viral envelope and a dominant immunogen. To analyze the...
Glycoproteins homologous to glycoprotein B (gB) of herpes simplex virus constitute the most highly conserved group of herpesvirus glycoproteins. This strong conservation of amino acid sequences might...
Identification and characterization of pseudorabies virus glycoprotein H.
Klupp, B G, Visser, N, Mettenleiter, T C
On the basis of DNA sequence analysis, it has recently been shown that the pseudorabies virus (PrV) genome encodes a protein homologous to glycoprotein H (gH) of other herpesviruses (B. Klupp and...
Pseudorabies virus glycoproteins gII and gp50 are essential for virus penetration.
Pseudorabies virus (PrV) glycoproteins gII and gp50 are major constituents of the viral envelope and targets of neutralizing monoclonal antibodies. Both are homologs of essential glycoproteins found...
Mettenleiter, T C, Zsak, L, Zuckermann, F, Sugg, N, Kern, H, Ben-Porat, T
Glycoprotein gIII is one of the major envelope glycoproteins of pseudorabies virus (PrV) (Suid herpesvirus 1). Although it is dispensable for viral growth, it has been shown to play a prominent role...
Zuckermann, F A, Zsak, L, Mettenleiter, T C, Ben-Porat, T
Pseudorabies virus (PrV) is the etiological agent of Aujeszky's disease, a disease that causes heavy economic losses in the swine industry. A rational approach to the generation of an effective...
Reilly, L M, Rall, G, Lomniczi, B, Mettenleiter, T C, Kuperschmidt, S, Ben-Porat, T
Pseudorabies virus is a herpesvirus which has a class 2 genome. However, under certain growth conditions it acquires a genome with class 3-like characteristics. In these variants, the leftmost...
Mettenleiter, T C, Lomniczi, B, Sugg, N, Schreurs, C, Ben-Porat, T
Several attenuated strains of pseudorabies virus contain genomes that carry a deletion in their short unique (Us) component. The sizes of the deletions are different in the various attenuated...
Low-level inversion of the L component of pseudorabies virus is not dependent on sequence homology.
Rall, G F, Kupershmidt, S, Lu, X Q, Mettenleiter, T C, Ben-Porat, T
Pseudorabies virus has a class 2 genome in which the S component is found in two orientations relative to the L component. The L component is bracketed by sequences that are partially homologous; it...
Zsak, L, Mettenleiter, T C, Sugg, N, Ben-Porat, T
The role of the nonessential glycoproteins gI, gp63, and gIII in the release of pseudorabies virus from different cell lines was investigated. We show that these glycoproteins may have a beneficial...
Pseudorabies virus avirulent strains fail to express a major glycoprotein.
Mettenleiter, T C, Lukàcs, N, Rziha, H J
The unique short (Us) region of the pseudorabies virus (PRV) genome, which displays high transcriptional activity during the late phase of infection and has been found to code for glycoproteins, is...
Glycoprotein gIII of pseudorabies virus is multifunctional.
Schreurs, C, Mettenleiter, T C, Zuckermann, F, Sugg, N, Ben-Porat, T
One of the major glycoproteins of pseudorabies virus, gIII, is nonessential for growth in cell culture. Mutants defective in gIII, however, consistently yield lower titers of infectious virus (3- to...
Complex between glycoproteins gI and gp63 of pseudorabies virus: its effect on virus replication.
Zuckermann, F A, Mettenleiter, T C, Schreurs, C, Sugg, N, Ben-Porat, T
To ascertain the biological functions of different glycoproteins that are nonessential for pseudorabies virus growth in vitro, we have constructed mutants defective in one (or a combination) of these...
Role of glycoprotein gIII of pseudorabies virus in virulence.
Mettenleiter, T C, Schreurs, C, Zuckermann, F, Ben-Porat, T, Kaplan, A S
Deletion mutants of pseudorabies virus unable to express glycoprotein gIII, gI, or gp63 or double and triple mutants defective in these glycoproteins were constructed, and their virulence for day-old...
Mettenleiter, T C, Lukacs, N, Rziha, H J
Cell-free translation of pseudorabies virus RNA isolated during the late phase of the infectious cycle yielded a variety of polypeptides. A monoclonal antibody directed against one of the major viral...
Lukàcs, N, Thiel, H J, Mettenleiter, T C, Rziha, H J
The glycoproteins of pseudorabies virus (PRV) Phylaxia were characterized with monoclonal antibodies as specific reagents. Three major structural glycoproteins with molecular weights of 155,000...
Role of pseudorabies virus glycoprotein gI in virus release from infected cells.
Mettenleiter, T C, Schreurs, C, Zuckermann, F, Ben-Porat, T
The Bartha vaccine strain of pseudorabies virus has a deletion in the short unique (Us) region of its genome which includes the genes that code for glycoproteins gI and gp63 (E. Petrovskis, J. G....
Role of a structural glycoprotein of pseudorabies in virus virulence.
Mettenleiter, T C, Zsak, L, Kaplan, A S, Ben-Porat, T, Lomniczi, B
The virulence of deletion mutants of pseudorabies virus defective in the expression of glycoprotein gI, gp63, or both was tested in 1-day-old chickens and young pigs. In the absence of expression of...