Sepkowitz, K A, Raffalli, J, Riley, L, Kiehn, T E, Armstrong, D
A resurgence of tuberculosis has occurred in recent years in the United States and abroad. Deteriorating public health services, increasing numbers of immigrants from countries of endemicity, and...
Saubolle, M A, Kiehn, T E, White, M H, Rudinsky, M F, Armstrong, D
Reports of the association of Mycobacterium haemophilum with disease in humans have greatly increased. At least 64 cases have now been reported, with symptoms ranging from focal lesions to...
Mayo, J B, Kiehn, T E, Wong, B, Bernard, E M, Armstrong, D
Seven Pseudomonas aeruginosa strains found to be susceptible to gentamicin by the Autobac I system and resistant by the Bauer-Kirby disk diffusion method were tested for the presence of mixed...
Comparison of the BACTEC System with Blind Subculture for the Detection of Bacteremia
Caslow, M., Ellner, P. D., Kiehn, T. E.
One thousand blood specimens were cultured in BACTEC vials containing modified Columbia broth in aerobic, anaerobic, and hypertonic formulations. Radiometric readings and subcultures were performed...
Evaluation of the Minitek System for Identification of Enterobacteriaceae
Kiehn, T. E., Brennan, K., Ellner, P. D.
Clinical isolates (869) and stock cultures (35) of Enterobacteriaceae were tested in parallel with the Minitek and conventional systems. The Minitek correctly identified 822 of 904 cultures. When a...
Activities of antimicrobial agents against clinical isolates of Mycobacterium haemophilum.
Bernard, E M, Edwards, F F, Kiehn, T E, Brown, S T, Armstrong, D
Mycobacterium haemophilum, first described in 1978, can cause severe infections of skin, respiratory tract, bone, and other organs of immunocompromised patients. There is no standardized...
Mycobacterium genavense infections in pet animals.
Kiehn, T E, Hoefer, H, Bottger, E C, Ross, R, Wong, M, Edwards, F, ...
Mycobacterium genavense, a recently reported cause of a wasting illness in patients with AIDS, was isolated from a cervical lymph mode from a dog with severe hind limb weakness and from trachael...
Restriction fragment length polymorphism analysis of clinical isolates of Mycobacterium haemophilum.
Kikuchi, K, Bernard, E M, Kiehn, T E, Armstrong, D, Riley, L W
Mycobacterium haemophilum is an emerging opportunistic pathogen, and since 1989, infections caused by this organism have been identified more frequently in the New York City area than in any other...
Blood cultures processed with a lysis-centrifugation (Isolator) system and a radiometric (BACTEC 13A) broth system were compared for the recovery of Mycobacterium avium-M. intracellulare from...
Ellner, P D, Kiehn, T E, Cammarata, R, Hosmer, M
The combination of radiometric methodology (BACTEC 12B) and probe technology for recovery and identification of mycobacteria was studied in two large hospital laboratories. The sediment from vials...
Evaluation of the Quantum II yeast identification system.
Kiehn, T E, Edwards, F F, Tom, D, Lieberman, G, Bernard, E M, Armstrong, D
We compared three methods for identifying clinical yeast isolates: Abbott Quantum II, API 20C, and a modified BBL Minitek system. The API 20C and modified Minitek systems agreed on the identification...
Disseminated mycobacterial infections are commonly seen in acquired immunodeficiency syndrome (AIDS) patients, and laboratory culture is the best method for diagnosing these infections. In addition...
Clinical comparison of lysis-centrifugation and radiometric resin systems for blood culture.
The Isolator 10 lysis-centrifugation blood culture system (E. I. du Pont de Nemours & Co., Inc., Wilmington, Del.) and the BACTEC 16B-17D radiometric resin system (Johnston Laboratories, Inc.,...
Specific DNA probes (Gen-Probe Corp., San Diego, Calif.) for Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium tuberculosis were compared with conventional methods for the...
Liver infection caused by Coniothyrium fuckelii in a patient with acute myelogenous leukemia.
Kiehn, T E, Polsky, B, Punithalingam, E, Edwards, F F, Brown, A E, Armstrong, D
A case of liver infection caused by Coniothyrium fuckelii is described in a patient with acute myelogenous leukemia. This fungus is found in the soil and can be a pathogen of plants. Coniothyrium...
Kiehn, T E, Wong, B, Edwards, F F, Armstrong, D
Blood cultures obtained with a lysis-centrifugation (L-C) system and a conventional two-bottle broth system were compared for the recovery of bacteria and yeasts from 7,000 cultures. The L-C system...
Routine aerobic terminal subculturing of blood cultures in a cancer hospital.
Kiehn, T E, Wong, B, Edwards, F F, Armstrong, D
Routine terminal aerobic subcultures of macroscopically negative blood culture bottles were evaluated during a 15-month period when 30,000 blood cultures were processed. Each blood culture set...
Whimbey, E, Wong, B, Kiehn, T E, Armstrong, D
The potential clinical value of colony counts determined by the lysis-centrifugation blood culture method was studied by reviewing the records of eight patients with persistent septicemia in whom...
Kiehn, T E, Edwards, F F, Brannon, P, Tsang, A Y, Maio, M, Gold, J W, ...
The Mycobacterium avium complex, only rarely described as an invasive pathogen in humans, has recently been reported to frequently cause disseminated disease in patients with the acquired immune...
Wachter, E, Brown, A E, Kiehn, T E, Lee, B J, Armstrong, D
A case of septicemia caused by Neisseria meningitidis serogroup 29E (Z') in a patient with plasma cell leukemia is described. The patient developed disseminated intravascular coagulation, had a...
Kiehn, T E, Nelson, P E, Bernard, E M, Edwards, F F, Koziner, B, Armstrong, D
A case of catheter-associated fungemia caused by Fusarium chlamydosporum is described in a patient with lymphocytic lymphoma. The fungus, which has been isolated from soil but not reported to cause...
Kiehn, T E, Gold, J W, Brannon, P, Timberger, R J, Armstrong, D
Mycobacterium tuberculosis was detected by the Isolator lysis-centrifugation blood culture system from the blood of a patient with tuberculosis of the breast. The organism also grew on conventional...
The Isolator 10 lysis-centrifugation blood culture system (E. I. du Pont de Nemours & Co., Inc., Wilmington, Del.) was compared with the BACTEC radiometric method (Johnston Laboratories, Inc.,...
Rate of arabinitol production by pathogenic yeast species.
Bernard, E M, Christiansen, K J, Tsang, S F, Kiehn, T E, Armstrong, D
D-Arabinitol is a five-carbon polyol that is produced by many fungi. Detection of the metabolite has been reported in serum from patients with invasive candidiasis. We studied the production and...
Species identification of coagulase-negative staphylococcal isolates from blood cultures.
Eng, R H, Wang, C, Person, A, Kiehn, T E, Armstrong, D
Coagulase-negative staphylococci generally are not fully identified, are called Staphylococcus epidermidis, and are considered contaminants when isolated from blood cultures. In a cancer hospital...
Kiehn, T E, Capitolo, C, Armstrong, D
Turbid broth (0.5 ml) from blood culture bottles was inoculated into 0.5 ml of brain heart infusion broth, incubated for 3 to 6 h, diluted 1:500 in distilled water, and then inoculated directly into...
Bone infection caused by debaryomyces hansenii in a normal host: a case report.
Wong, B, Kiehn, T E, Edwards, F, Bernard, E M, Marcove, R C, De Harven, E, ...
Debaryomyces hansenii was isolated from a cystic lesion in the distal tibia of a healthy 23-year-old woman. Ascospores were demonstrated when the organism was incubated at 25 but not at 30 degrees C....
Pneumonia caused by Cunninghamella bertholletiae complicating chronic lymphatic leukemia.
Kiehn, T E, Edwards, F, Armstrong, D, Rosen, P P, Weitzman, I
A case of pneumonia caused by Cunninghamella bertholletiae is described in a patient with chronic lymphatic leukemia. The species of Cunninghamella, a genus in the order Mucorales, are characterized...
Comparative recovery of fungi from biphasic and conventional blood culture media.
Kiehn, T E, Capitolo, C, Mayo, J B, Armstrong, D
A brain heart infusion broth and agar biphasic bottle was compared with a vented broth brain heart infusion bottle for the recovery of fungi from blood. A total of 40 fungi, all yeasts, were...
Critical analysis of hypertonic medium and agitation in detection of bacteremia.
Ellner, P D, Kiehn, T E, Beebe, J L, McCarthy, L R
Over 18,000 clinical specimens collected in Vacutainer tubes with sodium polyanethol sulfonate were inoculated into modified Columbia broth (MCB) with and without 10% sucrose. The effects of venting...
Sepkowitz, K A, Raffalli, J, Riley, L, Kiehn, T E, Armstrong, D
A resurgence of tuberculosis has occurred in recent years in the United States and abroad. Deteriorating public health services, increasing numbers of immigrants from countries of endemicity, and...
Saubolle, M A, Kiehn, T E, White, M H, Rudinsky, M F, Armstrong, D
Reports of the association of Mycobacterium haemophilum with disease in humans have greatly increased. At least 64 cases have now been reported, with symptoms ranging from focal lesions to...
Mayo, J B, Kiehn, T E, Wong, B, Bernard, E M, Armstrong, D
Seven Pseudomonas aeruginosa strains found to be susceptible to gentamicin by the Autobac I system and resistant by the Bauer-Kirby disk diffusion method were tested for the presence of mixed...
Comparison of the BACTEC System with Blind Subculture for the Detection of Bacteremia
Caslow, M., Ellner, P. D., Kiehn, T. E.
One thousand blood specimens were cultured in BACTEC vials containing modified Columbia broth in aerobic, anaerobic, and hypertonic formulations. Radiometric readings and subcultures were performed...
Evaluation of the Minitek System for Identification of Enterobacteriaceae
Kiehn, T. E., Brennan, K., Ellner, P. D.
Clinical isolates (869) and stock cultures (35) of Enterobacteriaceae were tested in parallel with the Minitek and conventional systems. The Minitek correctly identified 822 of 904 cultures. When a...
Activities of antimicrobial agents against clinical isolates of Mycobacterium haemophilum.
Bernard, E M, Edwards, F F, Kiehn, T E, Brown, S T, Armstrong, D
Mycobacterium haemophilum, first described in 1978, can cause severe infections of skin, respiratory tract, bone, and other organs of immunocompromised patients. There is no standardized...
Mycobacterium genavense infections in pet animals.
Kiehn, T E, Hoefer, H, Bottger, E C, Ross, R, Wong, M, Edwards, F, ...
Mycobacterium genavense, a recently reported cause of a wasting illness in patients with AIDS, was isolated from a cervical lymph mode from a dog with severe hind limb weakness and from trachael...
Restriction fragment length polymorphism analysis of clinical isolates of Mycobacterium haemophilum.
Kikuchi, K, Bernard, E M, Kiehn, T E, Armstrong, D, Riley, L W
Mycobacterium haemophilum is an emerging opportunistic pathogen, and since 1989, infections caused by this organism have been identified more frequently in the New York City area than in any other...
Blood cultures processed with a lysis-centrifugation (Isolator) system and a radiometric (BACTEC 13A) broth system were compared for the recovery of Mycobacterium avium-M. intracellulare from...
Ellner, P D, Kiehn, T E, Cammarata, R, Hosmer, M
The combination of radiometric methodology (BACTEC 12B) and probe technology for recovery and identification of mycobacteria was studied in two large hospital laboratories. The sediment from vials...
Evaluation of the Quantum II yeast identification system.
Kiehn, T E, Edwards, F F, Tom, D, Lieberman, G, Bernard, E M, Armstrong, D
We compared three methods for identifying clinical yeast isolates: Abbott Quantum II, API 20C, and a modified BBL Minitek system. The API 20C and modified Minitek systems agreed on the identification...
Disseminated mycobacterial infections are commonly seen in acquired immunodeficiency syndrome (AIDS) patients, and laboratory culture is the best method for diagnosing these infections. In addition...
Clinical comparison of lysis-centrifugation and radiometric resin systems for blood culture.
The Isolator 10 lysis-centrifugation blood culture system (E. I. du Pont de Nemours & Co., Inc., Wilmington, Del.) and the BACTEC 16B-17D radiometric resin system (Johnston Laboratories, Inc.,...
Specific DNA probes (Gen-Probe Corp., San Diego, Calif.) for Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium tuberculosis were compared with conventional methods for the...
Liver infection caused by Coniothyrium fuckelii in a patient with acute myelogenous leukemia.
Kiehn, T E, Polsky, B, Punithalingam, E, Edwards, F F, Brown, A E, Armstrong, D
A case of liver infection caused by Coniothyrium fuckelii is described in a patient with acute myelogenous leukemia. This fungus is found in the soil and can be a pathogen of plants. Coniothyrium...
Kiehn, T E, Wong, B, Edwards, F F, Armstrong, D
Blood cultures obtained with a lysis-centrifugation (L-C) system and a conventional two-bottle broth system were compared for the recovery of bacteria and yeasts from 7,000 cultures. The L-C system...
Routine aerobic terminal subculturing of blood cultures in a cancer hospital.
Kiehn, T E, Wong, B, Edwards, F F, Armstrong, D
Routine terminal aerobic subcultures of macroscopically negative blood culture bottles were evaluated during a 15-month period when 30,000 blood cultures were processed. Each blood culture set...
Whimbey, E, Wong, B, Kiehn, T E, Armstrong, D
The potential clinical value of colony counts determined by the lysis-centrifugation blood culture method was studied by reviewing the records of eight patients with persistent septicemia in whom...
Kiehn, T E, Edwards, F F, Brannon, P, Tsang, A Y, Maio, M, Gold, J W, ...
The Mycobacterium avium complex, only rarely described as an invasive pathogen in humans, has recently been reported to frequently cause disseminated disease in patients with the acquired immune...
Wachter, E, Brown, A E, Kiehn, T E, Lee, B J, Armstrong, D
A case of septicemia caused by Neisseria meningitidis serogroup 29E (Z') in a patient with plasma cell leukemia is described. The patient developed disseminated intravascular coagulation, had a...
Kiehn, T E, Nelson, P E, Bernard, E M, Edwards, F F, Koziner, B, Armstrong, D
A case of catheter-associated fungemia caused by Fusarium chlamydosporum is described in a patient with lymphocytic lymphoma. The fungus, which has been isolated from soil but not reported to cause...
Kiehn, T E, Gold, J W, Brannon, P, Timberger, R J, Armstrong, D
Mycobacterium tuberculosis was detected by the Isolator lysis-centrifugation blood culture system from the blood of a patient with tuberculosis of the breast. The organism also grew on conventional...
The Isolator 10 lysis-centrifugation blood culture system (E. I. du Pont de Nemours & Co., Inc., Wilmington, Del.) was compared with the BACTEC radiometric method (Johnston Laboratories, Inc.,...
Rate of arabinitol production by pathogenic yeast species.
Bernard, E M, Christiansen, K J, Tsang, S F, Kiehn, T E, Armstrong, D
D-Arabinitol is a five-carbon polyol that is produced by many fungi. Detection of the metabolite has been reported in serum from patients with invasive candidiasis. We studied the production and...
Species identification of coagulase-negative staphylococcal isolates from blood cultures.
Eng, R H, Wang, C, Person, A, Kiehn, T E, Armstrong, D
Coagulase-negative staphylococci generally are not fully identified, are called Staphylococcus epidermidis, and are considered contaminants when isolated from blood cultures. In a cancer hospital...
Kiehn, T E, Capitolo, C, Armstrong, D
Turbid broth (0.5 ml) from blood culture bottles was inoculated into 0.5 ml of brain heart infusion broth, incubated for 3 to 6 h, diluted 1:500 in distilled water, and then inoculated directly into...
Bone infection caused by debaryomyces hansenii in a normal host: a case report.
Wong, B, Kiehn, T E, Edwards, F, Bernard, E M, Marcove, R C, De Harven, E, ...
Debaryomyces hansenii was isolated from a cystic lesion in the distal tibia of a healthy 23-year-old woman. Ascospores were demonstrated when the organism was incubated at 25 but not at 30 degrees C....
Pneumonia caused by Cunninghamella bertholletiae complicating chronic lymphatic leukemia.
Kiehn, T E, Edwards, F, Armstrong, D, Rosen, P P, Weitzman, I
A case of pneumonia caused by Cunninghamella bertholletiae is described in a patient with chronic lymphatic leukemia. The species of Cunninghamella, a genus in the order Mucorales, are characterized...
Comparative recovery of fungi from biphasic and conventional blood culture media.
Kiehn, T E, Capitolo, C, Mayo, J B, Armstrong, D
A brain heart infusion broth and agar biphasic bottle was compared with a vented broth brain heart infusion bottle for the recovery of fungi from blood. A total of 40 fungi, all yeasts, were...
Critical analysis of hypertonic medium and agitation in detection of bacteremia.
Ellner, P D, Kiehn, T E, Beebe, J L, McCarthy, L R
Over 18,000 clinical specimens collected in Vacutainer tubes with sodium polyanethol sulfonate were inoculated into modified Columbia broth (MCB) with and without 10% sucrose. The effects of venting...