T. Fujiwara

Publication List Details

Period

1980 - 9999

Number

200

Co-Authors

Propagation of Two-Dimensional Nonsteady Detonation in a Channel with Backward-Facing Step, (9999)

Sugimura,T., Taki,S., Fujiwara,T.

Re-establishment or quenching of a detonation at a corner is conceivably caused by the interaction of numerous triple shock waves contained in the frontal structure of the detonation; a combined...

Shock Propagation in Liquid-Gas Media, (9999)

Fujiwara,T., Hasegawa,T.

Shock wave propagation in bubbled liquids is studied from experimental and theoretical points of view. The third-order partial differential equation derived through acoustic approximation is used to...

Topological stability of Majorana zero-modes in superconductor-topological insulator systems (2009)

Fukui, T., Fujiwara, T.

We derive an index theorem for zero-energy Majorana fermion modes in a superconductor-topological insulator system in both two and three dimensions, when the model has not only particle-hole symmetry...

A Z$_2$ index of Dirac operator with time reversal symmetry (2009)

Fukui, T., Fujiwara, T.

With time reversal symmetry a Dirac operator has vanishing index and Chern number. We show that we can nevertheless define a nontrivial Z$_2$ index as well as a corresponding topological invariant...

Quantization of non-Abelian Berry phase for time reversal invariant systems (2009)

Fukui, T., Fujiwara, T.

We present a quantized non-Abelian Berry phase for time reversal invariant systems such as quantum spin Hall effect. Ordinary Berry phase is defined by an integral of Berry's gauge potential along a...

$^{63/65}$Cu- and $^{35/37}$Cl-NMR Studies of Triplet Localization in the Quantum Spin System NH$_4$CuCl$_3$ (2009)

Inoue, H., Tani, S., Hosoya, S., Inokuchi, K., Fujiwara, T., Saito, T., ...

$^{63/65}$Cu- and $^{35/37}$Cl-NMR experiments were performed to investigate triplet localization in the $S=1/2$ dimer compound NH$_4$CuCl$_3$, which shows magnetization plateaus at one-quarter and...

Study of the decay K+ => pi+,nu,nubar in the momentum region 140 (2009)

Bassalleck, B., Bhuyan, B., Blackmore, E. W., Bryman, D. A., Chen, S., ...

Experiment E949 at Brookhaven National Laboratory has observed three new events consistent with the decay K+ => pi+,nu,nubar in the pion momentum region 140 < P_pi < 199 MeV/c in an exposure of...

On a weighted quasi-residual minimization strategy of the QMR method for solving complex symmetric shifted linear systems (2009)

Sogabe, T., Hoshi, T., Fujiwara, T.

We consider the solution of complex symmetric shifted linear systems. Such systems arise in large-scale electronic structure simulations and there is a strong need for the fast solution of the...

ACKNOWLEDGMENT (2008)

Y. Desaki, T. Fujiwara, T. Kasami

In Table I the first column contains the dimension of r, the second one contains the distance of the dual code of r (or a lower estimate), the following two columns contain the RES calculated as...

Topological meaning of Z$_2$ numbers in time reversal invariant systems (2008)

Fukui, T., Fujiwara, T., Hatsugai, Y.

We show that the Z$_2$ invariant, which classifies the topological properties of time reversal invariant insulators, has deep relationship with the global anomaly. Although the second Chern number is...

GW approximation with LSDA+U method and applications to NiO, MnO, and V$_2$O$_3$ (2008)

Kobayashi, S., Nohara, Y., Yamamoto, S., Fujiwara, T.

A GW approximation (GWA) method named U+GWA is proposed, where we can start GWA with more localized wave functions obtained by the local spin-density approximation (LSDA)+U method. Then GWA and U+GWA...

NMR Study on the Vortex Slush Phase in Organic Superconductor \kappa-(BEDT-TTF)2Cu(NCS)2 (2007)

Urano, M., Tonishi, J., Inoue, H., Saito, T., Fujiwara, T., Chiku, H., ...

The vortex state in a single crystal of the layered organic superconductor \kappa-(BEDT-TTF2Cu(NCS)2, where BEDT-TTF (or ET) is bis(ethylenedithio)tetrathiafulvalene, was studied by 1H-NMR. Under a...

Charge and spin stripe in La$_{2-x}$Sr$_{x}$NiO$_{4}$ (x=1/3,1/2) (2007)

Yamamoto, S., Fujiwara, T., Hatsugai, Y.

Electronic structure of stripe ordered La$_{2-x}$Sr$_{x}$NiO$_{4}$ is investigated. The system with x=1/3 is insulator, in LSDA+U calculations, and shows charge and spin stripe, consistent with the...

Cu-NMR study on the disordered quantum spin magnet with the Bose-glass ground state (2006)

Fujiwara, T., Inoue, H., Oosawa, A., Tsunoda, R., Goto, T., Suzuki, T., ...

Cu-NMR study has been performed on the disordered spin-gap system Tl1-xKxCuCl3 In the high-field H > HC=\Delta/\mu_B, where \Delta is the spin-gap, the hyperfine field becomes extremely inhomogeneous...

A numerical method for calculating the Green's function arising from electronic structure theory (2006)

Sogabe, T., Hoshi, T., Fujiwara, T.

We developed a fast numerical methodfor complex symmetric shifted linear systems, which is motivated by the quantum-mechanical (electronic-structure) theory in nanoscale materials. The method is...

Electronic structure of antiferromagnetic LaMnO3 and the effects of charge polarization (2006)

Nohara, Y., Yamasaki, A., Kobayashi, S., Fujiwara, T.

The electronic structure of A-type antiferromagnetic insulator LaMnO3 is investigated by the GW approximation. The band gap and spectrum are in a good agreement with experimental observation. The...

Electronic structure of A-type antiferomagnetic LaMnO_3 by GW approximation (2005)

Nohara, Y., Yamasaki, A., Kobayashi, S., Fujiwara, T.

The electronic structure of the A-type antiferromagnetic insulator LaMnO3 is investigated by the GW approximation. The band gap and spectrum are in a good agreement with experimental observation. The...

Large-scale electronic-structure theory and nanoscale defects formed in cleavage process of silicon (2005)

Hoshi, T., Takayama, R., Iguchi, Y., Fujiwara, T.

Several methods are constructed for large-scale electronic structure calculations. Test calculations are carried out with up to 10^7 atoms. As an application, cleavage process of silicon is...

Linear Algebraic Calculation of Green's function for Large-Scale Electronic Structure Theory (2005)

Takayama, R., Hoshi, T., Sogabe, T., Fujiwara, T.

A linear algebraic method named the shifted conjugate-orthogonal-conjugate-gradient method is introduced for large-scale electronic structure calculation. The method gives an iterative solver...

THE SPECTROSCOPY AND STRUCTURE OF THIOPHOSGENE (Cl$_2$CS) IN ITS SINGLET A$_2$ STATE: A HIGH RESOLUTION STUDY OF THE ROTATIONAL STRUCTURE OF THE n $\rightarrow \pi^*$ TRANSITION (2005)

Fujiwara, T., Lim, E. C., Judge, R. H., Kodet, John, Moule, D. C.

Author Institution: Department of Chemistry and Centre for Laser and Optical Spectroscopy, The University of Akron, Akron, OH 44325-3601 ; Department of Chemistry, University of Wisconsin-Parkside,...

Higher orders of the high-temperature expansion for the Ising model in three dimensions (2003)

Arisue, H., Fujiwara, T., Tabata, K.

The new algorithm of the finite lattice method is applied to generate the high-temperature expansion series of the simple cubic Ising model to $\beta^{50}$ for the free energy, to $\beta^{32}$ for...

Evolution and characterization of fluorite-like nano-$SrBi_2Nb_2O_9$ phase in the $SrO-Bi_2O_3-Nb_2O_5-Li_2B_4O_7$ glass system (2003)

Prasad, Syam N, Varma, KBR, Takahashi, Y, Benino, Y, Fujiwara, T, Komatsu, T

Transparent glasses of various compositions in the system $(100−x)Li_2B_4O_7 - x(SrO-Bi_2O_3-Nb_2O_5)$ (where x=10, 20, 30, 40, 50 and 60, in molar ratio) were fabricated via splat quenching...

Evolution and characterization of fluorite-like nano-$SrBi_2Nb_2O_9$ phase in the $SrO-Bi_2O_3-Nb_2O_5-Li_2B_4O_7$ glass system (2003)

Prasad, Syam N, Varma, KBR, Takahashi, Y, Benino, Y, Fujiwara, T, Komatsu, T

Transparent glasses of various compositions in the system $(100−x)Li_2B_4O_7 - x(SrO-Bi_2O_3-Nb_2O_5)$ (where x=10, 20, 30, 40, 50 and 60, in molar ratio) were fabricated via splat quenching...

New algorithm of the high-temperature expansion for the Ising model in three dimensions (2002)

Arisue, H., Fujiwara, T.

New algorithm of the finite lattice method is presented to generate the high-temperature expansion series of the Ising model. It enables us to obtain much longer series in three dimensions when...

Toward Construction of Exact Operator Solution of $A_N$-Toda Field Theory (2002)

Takimoto, Y., Fujiwara, T.

Quantum $A_N$-Toda field theory in two dimensions is investigated based on the method of quantizing canonical free field. Toda exponential operator associated with the fundamental weight $\lambda^1$...

Spectrum of the Hermitian Wilson-Dirac Operator for a Uniform Magnetic Field in Two Dimensions (2002)

Kurokawa, H., Fujiwara, T.

It is shown that the eigenvalue problem for the hermitian Wilson-Dirac operator of for a uniform magnetic field in two dimensions can be reduced to one-dimensional problem described by a relativistic...

A Numerical Study of Spectral Flows of Hermitian Wilson-Dirac Operator and the Index Theorem in Abelian Gauge Theories on Finite Lattices (2000)

Fujiwara, T.

We investigate the index of the Neuberger's Dirac operator in abelian gauge theories on finite lattices by numerically analyzing the spectrum of the hermitian Wilson-Dirac operator for a continuous...

Topological Obstruction in Block-spin Transformations (2000)

Fujiwara, T., Hayashi, T., Suzuki, H., Wu, K.

Block-spin transformations from a fine lattice to a coarse one are shown to give rise to a one-to-one correspondence between the zero-modes of the Ginsparg-Wilson Dirac operators. The index is then...

Topological Charge of Lattice Abelian Gauge Theory (2000)

Fujiwara, T., Suzuki, H., Wu, K.

Configuration space of abelian gauge theory on a periodic lattice becomes topologically disconnected by excising exceptional gauge field configurations. It is possible to define a U(1) bundle from...

Quantitative analysis of vascular to cardiac selectivity of L- and T-type voltage-operated calcium channel antagonists in human tissues (2000)

Angus, J, Sarsero, D, Fujiwara, T, Molenaar, P, Xi, Q

1. Classical L-type voltage-operated calcium channel (VOCC) antagonists dilate blood vessels, depress myocardial contractility and slow cardiac conduction. 2. We compared four L-type VOCC antagonists...

Quantitative analysis of vascular to cardiac selectivity of L- and T-type voltage-operated calcium channel antagonists in human tissues (2000)

Angus, J, Sarsero, D, Fujiwara, T, Molenaar, P, Xi, Q

1. Classical L-type voltage-operated calcium channel (VOCC) antagonists dilate blood vessels, depress myocardial contractility and slow cardiac conduction. 2. We compared four L-type VOCC antagonists...

The origin and formation of cuspy density profiles through violent relaxation of stellar systems (1999)

Hozumi, S., Burkert, A., Fujiwara, T.

It is shown that the cuspy density distributions observed in the cores of elliptical galaxies can be realized by dissipationless gravitational collapse. The initial models consist of power-law...

Deformation of Polysynthetically Twinned Crystals of TiAl with a Nearly Stoichiometric Composition. (1998)

Fujiwara, T., Nakamura, A., Hosomi, M., Nishitani, S. R., Shirai, Y.

Polysynthetically twinned crystals of TiAl with a nearly stoichiometric composition have been grown and deformed in compression at room temperature; the yield stress and deformation behavior depends...

Deformation of Intermetallic Compounds TiAl and Al3Ti. (1998)

Hosomi, M., Nakamura, A., Fujiwara, T., Oh, M. H., Nishitani, S. R.

Individual Al3Ti crystals and polysynthetically twinned TiAl crystals were grown, and deformation experiments were conducted; Al3Ti deformation behavior is strongly dependent on crystal orientation;...

Quantum Exchange Algebra and Exact Operator Solution of $A_2$-Toda Field Theory (1998)

Takimoto, Y., Igarashi, H., Kurokawa, H., Fujiwara, T.

Locality is analyzed for Toda field theories by noting novel chiral description in the conventional nonchiral formalism. It is shown that the canonicity of the interacting to free field mapping...

Fermi surfaces and anomalous transport in quasicrystals (1998)

Roche, S., Fujiwara, T.

Fermi surfaces of several quasicrystalline approximants are calculated by means of ab-initio methods which enable direct comparison with dHvA experiments. A criterion for anomalous metallic transport...

Exact Operator Solution of $A_2$-Toda Field Theory (1998)

Fujiwara, T., Igarashi, H., Takimoto, Y.

Quantum $A_2$-Toda field theory in two dimensions is investigated based on the method of quantizing canonical free field. Toda exponential operators associated with the fundamental weights are...

Human vascular to cardiac tissue selectivity of L- and T-type calcium channel antagonists (1998)

Sarsero, D, Fujiwara, T, Molenaar, P, Angus, JA

1 Voltage-operated calcium channel (VOCC) antagonists are effective antihypertensive and antianginal agents but they also depress myocardial contractility. 2 We compared four L-type calcium channel...

Human vascular to cardiac tissue selectivity of L- and T-type calcium channel antagonists (1998)

Sarsero, D, Fujiwara, T, Molenaar, P, Angus, JA

1 Voltage-operated calcium channel (VOCC) antagonists are effective antihypertensive and antianginal agents but they also depress myocardial contractility. 2 We compared four L-type calcium channel...

On the Three-Point Couplings in Toda Field Theory (1997)

Fujiwara, T., Igarashi, H., Takimoto, Y.

Correlation functions of Toda field vertices are investigated by applying the method of integrating zero-mode developed for Liouville theory. We generalize the relations among the zero-, two- and...

Exact Operator Solution for Liouville Theory with $q$ A Root of Unity (1997)

Fujiwara, T., Igarashi, H., Takimoto, Y.

The exact operator solution for quantum Liouville theory constructed for the generic quantum deformation parameter $q$ is extended to the case with $q$ being a root of unity. The screening charge...

Enzymatic Iron Oxidation and Reduction in Magnetite Synthesizing Magnetospirillum Magnetotacticum (1997)

Fukumori, Y., Oyanagi, H., Yoshimatsu, K., Noguchi, Y., Fujiwara, T.

We investigated the enzymatic reduction and oxidation of iron in M.magnetotacticum which synthesizes magnetite at room temperature. NADH-Fe(III) reductase with the molecular mass of 36kDa was...

Enzymatic Iron Oxidation and Reduction in Magnetite Synthesizing Magnetospirillum Magnetotacticum (1997)

Fukumori, Y., Oyanagi, H., Yoshimatsu, K., Noguchi, Y., Fujiwara, T.

We investigated the enzymatic reduction and oxidation of iron in M.magnetotacticum which synthesizes magnetite at room temperature. NADH-Fe(III) reductase with the molecular mass of 36kDa was...

Enzymatic Iron Oxidation and Reduction in Magnetite Synthesizing Magnetospirillum Magnetotacticum (1997)

Fukumori, Y., Oyanagi, H., Yoshimatsu, K., Noguchi, Y., Fujiwara, T.

We investigated the enzymatic reduction and oxidation of iron in M.magnetotacticum which synthesizes magnetite at room temperature. NADH-Fe(III) reductase with the molecular mass of 36kDa was...

Quantum Exchange Algebra and Locality in Liouville Theory (1996)

Fujiwara, T., Igarashi, H., Takimoto, Y.

Exact operator solution for quantum Liouville theory is investigated based on the canonical free field. Locality, the field equation and the canonical commutation relations are examined based on the...

Growth of Velocity Dispersions for Collapsing Spherical Stellar Systems (1996)

Hozumi, S., Fujiwara, T., Kan-ya, Y.

First, we have ensured that spherical nonrotating collisionless systems collapse with almost retaining spherical configurations during initial contraction phases even if they are allowed to collapse...

BFV-BRST Quantization of 2D Supergravity (1995)

Fujiwara, T., Igarashi, Y., Kuriki, R., Tabei, T.

Two-dimensional supergravity theory is quantized as an anomalous gauge theory. In the Batalin-Fradkin (BF) formalism, the anomaly-canceling super-Liouville fields are introduced to identify the...

New insight into BRST anomalies in superstring theory (1994)

Fujiwara, T., Igarashi, Y., Koseki, M., Kuriki, R., Tabei, T.

Based on the extended BRST formalism of Batalin, Fradkin and Vilkovisky, we perform a general algebraic analysis of the BRST anomalies in superstring theory of Neveu-Schwarz-Ramond. Consistency...

CHANGES IN CENTRAL OPIOID RECEPTOR BINDING IN RELATION TO INFLAMMATION AND PAIN IN PATIENTS WITH RHEUMATOID ARTHRITIS (1994)

JONES, A. K. P., CUNNINGHAM, V. J., HA-KAWA, S., FUJIWARA, T., LUTHRA, S. K., SILVA, S., ...

A group of four patients with RA were examined to test the hypothesis that there is a change in the endogenous opioid system in the brain during inflammatory pain. Regional cerebral opioid receptor...

Gauge Equivalence in Two--Dimensional Gravity (1993)

Fujiwara, T., Igarashi, Y., Kubo, J., Tabei, T.

Two-dimensional quantum gravity is identified as a second-class system which we convert into a first-class system via the Batalin-Fradkin (BF) procedure. Using the extended phase space method, we...

Unrecognizable Black Holes in Two Dimensions (1993)

Fujiwara, T., Igarashi, Y., Kubo, J.

The classical 2D cosmological model of Callan, Giddings, Harvey and Strominger possesses a global symmetry that is responsible for decoupling of matter fields. The model is quantized on the basis of...

Canonical treatment of two dimensional gravity as an anomalous gauge theory (1993)

Fujiwara, T., Tabei, T., Igarashi, Y., Maeda, K., Kubo, J.

The extended phase space method of Batalin, Fradkin and Vilkovisky is applied to formulate two dimensional gravity in a general class of gauges. A BRST formulation of the light-cone gauge is...

A new insight into BRST anomalies in string theory (1992)

Fujiwara, T., Igarashi, Y., Kubo, J., Maeda, K.

Using the generalized hamiltonian method of Batalin, Fradkin and Vilkovisky, we investigate the algebraic structure of anomalies in the Polyakov string theory that appear as the Schwinger terms in...

AMORPHOUS ALLOY Fe-P : STRUCTURAL MODEL, VIBRATIONAL SPECTRA AND ELECTRONIC STATES (1980)

Fujiwara, T., Ishii, Y.

The relaxed structural models of amorphous metallic alloy Fe100-x Px have been constructed and analyzed . The pair atomic potentials of Morse-type are used for relaxing the DRPHS system consisted of...

AMORPHOUS ALLOY Fe-P : STRUCTURAL MODEL, VIBRATIONAL SPECTRA AND ELECTRONIC STATES (1980)

Fujiwara, T., Ishii, Y.

The relaxed structural models of amorphous metallic alloy Fe100-x Px have been constructed and analyzed . The pair atomic potentials of Morse-type are used for relaxing the DRPHS system consisted of...

AMORPHOUS ALLOY Fe-P : STRUCTURAL MODEL, VIBRATIONAL SPECTRA AND ELECTRONIC STATES (1980)

Fujiwara, T., Ishii, Y.

The relaxed structural models of amorphous metallic alloy Fe100-x Px have been constructed and analyzed . The pair atomic potentials of Morse-type are used for relaxing the DRPHS system consisted of...

Substrate recognition by Escherichia coli MutY using substrate analogs.

Chepanoske, C L, Porello, S L, Fujiwara, T, Sugiyama, H, David, S S

The Escherichia coli adenine glycosylase MutY is involved in the repair of 7,8-dihydro-8-oxo-2"-deoxyguanosine (OG):A and G:A mispairs in DNA. Our approach toward understanding recognition and...

Simple and Fast Lateral Flow Test for Classification of Leprosy Patients and Identification of Contacts with High Risk of Developing Leprosy

Bührer-Sékula, S., Smits, H. L., Gussenhoven, G. C., Van Leeuwen, J., Amador, S., Fujiwara, T., ...

The interruption of leprosy transmission is one of the main challenges for leprosy control programs since no consistent evidence exists that transmission has been reduced after the introduction of...

bor1-1, an Arabidopsis thaliana mutant that requires a high level of boron.

Noguchi, K, Yasumori, M, Imai, T, Naito, S, Matsunaga, T, Oda, H, ...

bor1-1 (high boron requiring), an Arabidopsis thaliana mutant that requires a high level of B, was isolated. When the B concentration in the medium was reduced to 3 microM, the expansion of rosette...

Cell-to-Cell Trafficking of Macromolecules through Plasmodesmata Potentiated by the Red Clover Necrotic Mosaic Virus Movement Protein.

Fujiwara, T, Giesman-Cookmeyer, D, Ding, B, Lommel, SA, Lucas, WJ

Direct evidence is presented for cell-to-cell trafficking of macromolecules via plasmodesmata in higher plants. The fluorescently labeled 35-kD movement protein of red clover necrotic mosaic virus...

Isolation of an Arabidopsis thaliana Mutant, mto1, That Overaccumulates Soluble Methionine (Temporal and Spatial Patterns of Soluble Methionine Accumulation).

Inaba, K., Fujiwara, T., Hayashi, H., Chino, M., Komeda, Y., Naito, S.

We isolated Arabidopsis thaliana mutants that are resistant to ethionine, a toxic analog of methionine (Met). One of the mutants was analyzed further, and it accumulated 10- to 40-fold more soluble...

Cloning and characterization of the fmt gene which affects the methicillin resistance level and autolysis in the presence of triton X-100 in methicillin-resistant Staphylococcus aureus.

Komatsuzawa, H, Sugai, M, Ohta, K, Fujiwara, T, Nakashima, S, Suzuki, J, ...

In methicillin-resistant Staphylococcus aureus (MRSA) strains, Triton X-100 reduced the oxacillin resistance level, although the degree of reduction varied from strain to strain. To study the...

Preparation of a monoclonal antibody specific for Entamoeba dispar and its ability to distinguish E. dispar from E. histolytica.

Tachibana, H, Kobayashi, S, Kaneda, Y, Takeuchi, T, Fujiwara, T

A monoclonal antibody (MAb), MAb ED17 (immunoglobulin G2a [IgG2a]), prepared against trophozoites of Entamoeba dispar SAW1734RclAR cultured monoxenically with Crithidia fasciculata, reacted with 25...

Cysteine protease of Porphyromonas gingivalis 381 enhances binding of fimbriae to cultured human fibroblasts and matrix proteins.

Kontani, M, Ono, H, Shibata, H, Okamura, Y, Tanaka, T, Fujiwara, T, ...

It has been shown that Porphyromonas gingivalis 381, a suspected periodontopathogen, possesses fimbriae on its cell surface. The organism is also known to produce proteases which can degrade the host...

In vitro inhibition of Giardia lamblia and Trichomonas vaginalis growth by bithionol, dichlorophene, and hexachlorophene.

Takeuchi, T, Kobayashi, S, Tanabe, M, Fujiwara, T

Bithionol, dichlorophene, and hexachlorophene, which are used in treating some helminthic infections, killed trophozoites of Giardia lamblia and Trichomonas vaginalis in modified BI-S-33 and Asami...

Cytochrome cb-type nitric oxide reductase with cytochrome c oxidase activity from Paracoccus denitrificans ATCC 35512.

Fujiwara, T, Fukumori, Y

A highly active nitric oxide reductase was purified from Paracoccus denitrificans ATCC 35512, formerly named Thiosphaera pantotropha, which was anaerobically cultivated in the presence of nitrate....

Succinate:quinone oxidoreductase (complex II) containing a single heme b in facultative alkaliphilic Bacillus sp. strain YN-2000.

Qureshi, M H, Fujiwara, T, Fukumori, Y

Succinate:quinone oxidoreductase (EC 1.3.5.1) was first purified from the facultative alkaliphilic Bacillus sp. strain YN-2000 in the presence of Triton X-100. The isolated enzyme showed high...

Purification and molecular characterization of glycylglycine endopeptidase produced by Staphylococcus capitis EPK1.

Sugai, M, Fujiwara, T, Akiyama, T, Ohara, M, Komatsuzawa, H, Inoue, S, ...

A novel staphylolytic enzyme, ALE-1, acting on Staphylococcus aureus, was purified from a Staphylococcus capitis EPK1 culture supernatant. The optimal pH range for staphylolytic activity was 7 to 9....

epr, which encodes glycylglycine endopeptidase resistance, is homologous to femAB and affects serine content of peptidoglycan cross bridges in Staphylococcus capitis and Staphylococcus aureus.

Sugai, M, Fujiwara, T, Ohta, K, Komatsuzawa, H, Ohara, M, Suginaka, H

Staphylococcus capitis EPK1 produces a glycylglycine endopeptidase, ALE-1 (M. Sugai, T. Fujiwara, T. Akiyama, M. Ohara, H. Komatsuzawa, S. Inoue, and H. Suginaka, J. Bacteriol. 179:1193-1202, 1997),...

Adhalin gene mutations in patients with autosomal recessive childhood onset muscular dystrophy with adhalin deficiency.

Kawai, H, Akaike, M, Endo, T, Adachi, K, Inui, T, Mitsui, T, ...

Homozygous adhalin gene mutations were found in three patients from two consanguineous families with autosomal recessive childhood onset muscular dystrophy. Muscle biopsies from patients in each...

Different roles of bases within the integration signal sequence of human immunodeficiency virus type 1 in vitro.

Yoshinaga, T, Fujiwara, T

To investigate the roles of bases near the tips of each strand of the long terminal repeat of the human immunodeficiency virus type 1 in the integration reaction, we examined the efficiencies of both...

Nucleotide sequence of the hag gene encoding flagellin of Escherichia coli.

Kuwajima, G, Asaka, J, Fujiwara, T, Fujiwara, T, Node, K, Kondo, E

We determined the DNA sequence of the hag gene of Escherichia coli K-12 and deduced the primary structure of the flagellin consisting of 497 amino acid residues. Comparison of the amino acid sequence...

Wild-type human p53 and a temperature-sensitive mutant induce Fas/APO-1 expression.

Owen-Schaub, L B, Zhang, W, Cusack, J C, Angelo, L S, Santee, S M, Fujiwara, T, ...

Fas/APO-1 is a cell surface protein known to trigger apoptosis upon specific antibody engagement. Because wild-type p53 can activate transcription as well as induce apoptosis, we queried whether p53...

Detection and characterization of a functional complex of human immunodeficiency virus type 1 integrase and its DNA substrate by UV cross-linking.

Yoshinaga, T, Kimura-Ohtani, Y, Fujiwara, T

To analyze the association of the human immunodeficiency virus type 1 integrase (IN) protein with DNA substrates, we applied a shortwave UV cross-linking method to the in vitro integration system....

Oral passive immunization against dental caries in rats by use of hen egg yolk antibodies specific for cell-associated glucosyltransferase of Streptococcus mutans.

Hamada, S, Horikoshi, T, Minami, T, Kawabata, S, Hiraoka, J, Fujiwara, T, ...

The effect of polyclonal egg yolk immunoglobulin G antibodies (yIgG) raised against whole cells, cell-free (CF) glucosyltransferase (GTase), or cell-associated (CA) GTase of serotype c Streptococcus...

Biochemical and immunobiological properties of lipopolysaccharide (LPS) from Bacteroides gingivalis and comparison with LPS from Escherichia coli.

Koga, T, Nishihara, T, Fujiwara, T, Nisizawa, T, Okahashi, N, Noguchi, T, ...

Lipopolysaccharides (LPSs) were isolated from Bacteroides gingivalis and Escherichia coli by the phenol-water and butanol-water procedures. The phenol-water-extracted LPS from B. gingivalis 381 was...

Chemical synthesis and serology of disaccharides and trisaccharides of phenolic glycolipid antigens from the leprosy bacillus and preparation of a disaccharide protein conjugate for serodiagnosis of leprosy.

Fujiwara, T, Hunter, S W, Cho, S N, Aspinall, G O, Brennan, P J

We examined the structural requirements within the species-specific 3,6-di-O-methyl-beta-D-glucopyranosyl-(1 leads to 4)-2,3-di-O-methyl- alpha-L-rhamnopyranosyl-(1 leads to...

Isolation of the Mycobacterium leprae-specific glycolipid antigen, phenolic glycolipid-I, from formalin-fixed human lepromatous liver.

Izumi, S, Sugiyama, K, Fujiwara, T, Hunter, S W, Brennan, P J

A Mycobacterium leprae-specific phenolic glycolipid antigen was purified from Formalin-fixed liver preserved from an advanced lepromatous leprosy patient. Its chemical and immunological properties...

Novel gelatin particle agglutination test for serodiagnosis of leprosy in the field.

Izumi, S, Fujiwara, T, Ikeda, M, Nishimura, Y, Sugiyama, K, Kawatsu, K

We developed a novel gelatin particle agglutination test (MLPA) for the serodiagnosis of leprosy; this test is especially useful for clinical practice and epidemiological surveys of leprosy in...

CDNA cloning of p112, the largest regulatory subunit of the human 26s proteasome, and functional analysis of its yeast homologue, sen3p.

Yokota, K, Kagawa, S, Shimizu, Y, Akioka, H, Tsurumi, C, Noda, C, ...

The 26S proteasome is a large multisubunit protease complex, the largest regulatory subunit of which is a component named p112. Molecular cloning of cDNA encoding human p112 revealed a polypeptide...

Microbial glycolipids: possible virulence factors that scavenge oxygen radicals.

Chan, J, Fujiwara, T, Brennan, P, McNeil, M, Turco, S J, Sibille, J C, ...

Two important pathogens of developing countries, Mycobacterium leprae, the etiologic agent of leprosy, and Leishmania donovani, the protozoal parasite that causes kalaazar, persist in the human host...

Integration of mini-retroviral DNA: a cell-free reaction for biochemical analysis of retroviral integration.

Fujiwara, T, Craigie, R

After retroviral infection of a permissive cell, the viral RNA is reverse-transcribed to make a DNA copy of the viral genome. Integration of this DNA copy into the host genome is a necessary step for...

Molecular design of a eukaryotic messenger RNA and its chemical synthesis.

Iwase, R, Maeda, M, Fujiwara, T, Sekine, M, Hata, T, Miura, K

A designed mRNA consisting of 42 ribonucleotides having the cap structure was synthesized. The capped leader sequence of the brome mosaic virus (BMV) mRNA 4, m7G5'pppGUAUUAAUA (F-1), was synthesized...

A novel guanine-guanine base pairing: crystal structure of a complex between 7-methylguanosine and its iodide.

Yamagata, Y, Fukumoto, S, Hamada, K, Fujiwara, T, Tomita, K

7-Methylguanosine, one of the biologically important minor nucleosides, could be crystallized as a complex of its zwitterionic form and its iodide, and the crystal structure was determined by the...

Reinvestigation of deoxyribonucleoside phosphorothioites: synthesis and properties of deoxyribonucleoside-3' dimethyl phosphites.

Nagai, H, Fujiwara, T, Fujii, M, Sekine, M, Hata, T

Further investigation of the synthesis of deoxyribonucleoside-3' (t-butyl) O-(2-cyanoethyl) phosphorothioites as monomer building units for the phosphorothioite approach has led us to conclude that...

Structural studies on the two forms of 8-bromo-2',3'-O-isopropylideneadenosine.

Fujii, S, Fujiwara, T, Tomita, K

The crystal and molecular structures of two forms of 8-bromo-2',3'-O-isopropylideneadenosine have been determined by X-ray methods. In one form, the molecular structure has planar conformation in the...

A downstream target of RHO1 small GTP-binding protein is PKC1, a homolog of protein kinase C, which leads to activation of the MAP kinase cascade in Saccharomyces cerevisiae.

Nonaka, H, Tanaka, K, Hirano, H, Fujiwara, T, Kohno, H, Umikawa, M, ...

The RHO1 gene in Saccharomyces cerevisiae encodes a homolog of the mammalian RhoA small GTP-binding protein, which is implicated in various actin cytoskeleton-dependent cell functions. In yeast,...

Bni1p implicated in cytoskeletal control is a putative target of Rho1p small GTP binding protein in Saccharomyces cerevisiae.

Kohno, H, Tanaka, K, Mino, A, Umikawa, M, Imamura, H, Fujiwara, T, ...

The RHO1 gene encodes a homolog of mammalian RhoA small GTP binding protein in the yeast Saccharomyces cerevisiae. Rho1p is localized at the growth sites, including the bud tip and the cytokinesis...

Troglitazone increases the number of small adipocytes without the change of white adipose tissue mass in obese Zucker rats.

Okuno, A, Tamemoto, H, Tobe, K, Ueki, K, Mori, Y, Iwamoto, K, ...

Troglitazone (CS-045) is one of the thiazolidinediones that activate the peroxisome proliferator-activated receptor gamma (PPARgamma), which is expressed primarily in adipose tissues. To elucidate...

Three-dimensional structure of fungal proteinase K reveals similarity to bacterial subtilisin.

Pähler, A, Banerjee, A, Dattagupta, J K, Fujiwara, T, Lindner, K, Pal, G P, ...

The three-dimensional structure of the fungal serine protease proteinase K has been determined at 3.3 A resolution by single crystal X-ray diffraction analysis. The enzyme crystallizes in the...

Brefeldin A arrests the intracellular transport of viral envelope proteins in primary cultured rat hepatocytes and HepG2 cells.

Oda, K, Fujiwara, T, Ikehara, Y

We have studied the effect of brefeldin A (BFA) on the intracellular transport of the envelope proteins of vesicular stomatitis virus (VSV) and sindbis virus in primary cultured rat hepatocytes. BFA...

X-ray diffraction studies of enkephalins. Crystal structure of [(4'-bromo) Phe4,Leu5]enkephalin.

Ishida, T, Kenmotsu, M, Mino, Y, Inoue, M, Fujiwara, T, Tomita, K, ...

In order to investigate the structure-activity relationship of [Leu5]- and [Met5]enkephalins, [(4'-bromo)Phe4, Leu5]-, [(4'-bromo)Phe4, Met5]- and [Met5] enkephalins were synthesized and...

Microbiological degradation of bile acids. The conjugation of a certain cholic acid metabolite with amino acids in Corynebacterium equi.

Hayakawa, S, Fujiwara, T, Kako, H

1. (4R)-4[4alpha-(2-Carboxyethyl)-3aalpha-hexahydro-7abeta-methyl-5-oxoindan-1beta-yl]valeric acid (II) could not be utilized by Arthrobacter simplex, even though the acid was one of the metabolites...

Microbiological degradation of bile acids, further degradation of a cholic acid metabolite containing the hexahydroindane nucleus by Corynebacterium equi.

Hayakawa, S, Fujiwara, T

1. The further degradation of a cholic acid (I) metabolite, (4R)-4-[4alpha-(2-carboxyethyl)-3aalpha-hexahydro-7abeta-methyl-5-oxoindan-1beta-yl]valeric acid (IIa), by Corynebacterium equi was...

Microbiological degradation of bile acids. The preparation of hexahydroindane derivatives as substrates for studying cholic acid degradation.

Hayakawa, S, Takata, T, Fujiwara, T, Hashimoto, S

Relatively large amounts of 3-(3aalpha-hexahydro-7abeta-methyl-1,5-dioxoindan-4alpha-yl)propionic acid (IIb), which is believed to be one of the intermediates involved in the degradation of cholic...

Microbiological degradation of bile acids. The preparation of some hypothetical metabolites involved in cholic acid degradation.

Hayakawa, S, Kanematsu, Y, Fujiwara, T, Kako, H

1. To identify the intermediates involved in the degradation of cholic acid, the further degradation of (4R)-4-[4alpha-(2-carboxyethyl)-3aalpha-hexahydro-7abeta-methyl-5-oxoindan-1beta-yl]valeric...

Bip/GRP78 but not calnexin associates with a precursor of glycosylphosphatidylinositol-anchored protein.

Oda, K, Wada, I, Takami, N, Fujiwara, T, Misumi, Y, Ikehara, Y

When fused in-frame with a C-terminal propeptide of placental alkaline phosphatase (PLAP), rat alpha 2u-globulin (alpha GL), a nonglycosylated secretory protein, was expressed on the cell surface as...

Crystal structure combined with genetic analysis of the Thermus thermophilus ribosome recycling factor shows that a flexible hinge may act as a functional switch.

Toyoda, T, Tin, O F, Ito, K, Fujiwara, T, Kumasaka, T, Yamamoto, M, ...

Ribosome recycling factor (RRF), in concert with elongation factor EF-G, is required for disassembly of the posttermination complex of the ribosome after release of polypeptides. The crystal...

Functional mapping of ribosome-contact sites in the ribosome recycling factor: a structural view from a tRNA mimic.

Fujiwara, T, Ito, K, Nakamura, Y

Ribosome recycling factor (RRF) is required for disassembly of the posttermination complex of the ribosome after release of polypeptides. The crystal structure of RRF resembles a tRNA shape, with an...

Specific acceptance of fetal bowel allograft in mice after combined treatment with anti-intercellular adhesion molecule-1 and leukocyte function-associated antigen-1 antibodies.

Kato, Y, Yamataka, A, Yagita, H, Okumura, K, Fujiwara, T, Miyano, T

OBJECTIVE: The aim of this study was to see whether tolerance could be induced by simultaneous administration of monoclonal antibodies (MoAbs) to intercellular adhesion molecule-1 (ICAM-1) and...

Effects of guanosine nucleotides on skinned smooth muscle tissue of the rabbit mesenteric artery.

Fujiwara, T, Itoh, T, Kubota, Y, Kuriyama, H

1. Effects of guanosine triphosphate (GTP) and guanosine 5'-o-(3-thio)triphosphate (GTP gamma S) on mechanical properties of skinned smooth muscle tissues of the rabbit mesenteric artery were...

Substrate recognition by Escherichia coli MutY using substrate analogs.

Chepanoske, C L, Porello, S L, Fujiwara, T, Sugiyama, H, David, S S

The Escherichia coli adenine glycosylase MutY is involved in the repair of 7,8-dihydro-8-oxo-2"-deoxyguanosine (OG):A and G:A mispairs in DNA. Our approach toward understanding recognition and...

Simple and Fast Lateral Flow Test for Classification of Leprosy Patients and Identification of Contacts with High Risk of Developing Leprosy

Bührer-Sékula, S., Smits, H. L., Gussenhoven, G. C., Van Leeuwen, J., Amador, S., Fujiwara, T., ...

The interruption of leprosy transmission is one of the main challenges for leprosy control programs since no consistent evidence exists that transmission has been reduced after the introduction of...

bor1-1, an Arabidopsis thaliana mutant that requires a high level of boron.

Noguchi, K, Yasumori, M, Imai, T, Naito, S, Matsunaga, T, Oda, H, ...

bor1-1 (high boron requiring), an Arabidopsis thaliana mutant that requires a high level of B, was isolated. When the B concentration in the medium was reduced to 3 microM, the expansion of rosette...

Cell-to-Cell Trafficking of Macromolecules through Plasmodesmata Potentiated by the Red Clover Necrotic Mosaic Virus Movement Protein.

Fujiwara, T, Giesman-Cookmeyer, D, Ding, B, Lommel, SA, Lucas, WJ

Direct evidence is presented for cell-to-cell trafficking of macromolecules via plasmodesmata in higher plants. The fluorescently labeled 35-kD movement protein of red clover necrotic mosaic virus...

Isolation of an Arabidopsis thaliana Mutant, mto1, That Overaccumulates Soluble Methionine (Temporal and Spatial Patterns of Soluble Methionine Accumulation).

Inaba, K., Fujiwara, T., Hayashi, H., Chino, M., Komeda, Y., Naito, S.

We isolated Arabidopsis thaliana mutants that are resistant to ethionine, a toxic analog of methionine (Met). One of the mutants was analyzed further, and it accumulated 10- to 40-fold more soluble...

Cloning and characterization of the fmt gene which affects the methicillin resistance level and autolysis in the presence of triton X-100 in methicillin-resistant Staphylococcus aureus.

Komatsuzawa, H, Sugai, M, Ohta, K, Fujiwara, T, Nakashima, S, Suzuki, J, ...

In methicillin-resistant Staphylococcus aureus (MRSA) strains, Triton X-100 reduced the oxacillin resistance level, although the degree of reduction varied from strain to strain. To study the...

Preparation of a monoclonal antibody specific for Entamoeba dispar and its ability to distinguish E. dispar from E. histolytica.

Tachibana, H, Kobayashi, S, Kaneda, Y, Takeuchi, T, Fujiwara, T

A monoclonal antibody (MAb), MAb ED17 (immunoglobulin G2a [IgG2a]), prepared against trophozoites of Entamoeba dispar SAW1734RclAR cultured monoxenically with Crithidia fasciculata, reacted with 25...

Cysteine protease of Porphyromonas gingivalis 381 enhances binding of fimbriae to cultured human fibroblasts and matrix proteins.

Kontani, M, Ono, H, Shibata, H, Okamura, Y, Tanaka, T, Fujiwara, T, ...

It has been shown that Porphyromonas gingivalis 381, a suspected periodontopathogen, possesses fimbriae on its cell surface. The organism is also known to produce proteases which can degrade the host...

In vitro inhibition of Giardia lamblia and Trichomonas vaginalis growth by bithionol, dichlorophene, and hexachlorophene.

Takeuchi, T, Kobayashi, S, Tanabe, M, Fujiwara, T

Bithionol, dichlorophene, and hexachlorophene, which are used in treating some helminthic infections, killed trophozoites of Giardia lamblia and Trichomonas vaginalis in modified BI-S-33 and Asami...

Cytochrome cb-type nitric oxide reductase with cytochrome c oxidase activity from Paracoccus denitrificans ATCC 35512.

Fujiwara, T, Fukumori, Y

A highly active nitric oxide reductase was purified from Paracoccus denitrificans ATCC 35512, formerly named Thiosphaera pantotropha, which was anaerobically cultivated in the presence of nitrate....

Succinate:quinone oxidoreductase (complex II) containing a single heme b in facultative alkaliphilic Bacillus sp. strain YN-2000.

Qureshi, M H, Fujiwara, T, Fukumori, Y

Succinate:quinone oxidoreductase (EC 1.3.5.1) was first purified from the facultative alkaliphilic Bacillus sp. strain YN-2000 in the presence of Triton X-100. The isolated enzyme showed high...

Purification and molecular characterization of glycylglycine endopeptidase produced by Staphylococcus capitis EPK1.

Sugai, M, Fujiwara, T, Akiyama, T, Ohara, M, Komatsuzawa, H, Inoue, S, ...

A novel staphylolytic enzyme, ALE-1, acting on Staphylococcus aureus, was purified from a Staphylococcus capitis EPK1 culture supernatant. The optimal pH range for staphylolytic activity was 7 to 9....

epr, which encodes glycylglycine endopeptidase resistance, is homologous to femAB and affects serine content of peptidoglycan cross bridges in Staphylococcus capitis and Staphylococcus aureus.

Sugai, M, Fujiwara, T, Ohta, K, Komatsuzawa, H, Ohara, M, Suginaka, H

Staphylococcus capitis EPK1 produces a glycylglycine endopeptidase, ALE-1 (M. Sugai, T. Fujiwara, T. Akiyama, M. Ohara, H. Komatsuzawa, S. Inoue, and H. Suginaka, J. Bacteriol. 179:1193-1202, 1997),...

Adhalin gene mutations in patients with autosomal recessive childhood onset muscular dystrophy with adhalin deficiency.

Kawai, H, Akaike, M, Endo, T, Adachi, K, Inui, T, Mitsui, T, ...

Homozygous adhalin gene mutations were found in three patients from two consanguineous families with autosomal recessive childhood onset muscular dystrophy. Muscle biopsies from patients in each...

Different roles of bases within the integration signal sequence of human immunodeficiency virus type 1 in vitro.

Yoshinaga, T, Fujiwara, T

To investigate the roles of bases near the tips of each strand of the long terminal repeat of the human immunodeficiency virus type 1 in the integration reaction, we examined the efficiencies of both...

Nucleotide sequence of the hag gene encoding flagellin of Escherichia coli.

Kuwajima, G, Asaka, J, Fujiwara, T, Fujiwara, T, Node, K, Kondo, E

We determined the DNA sequence of the hag gene of Escherichia coli K-12 and deduced the primary structure of the flagellin consisting of 497 amino acid residues. Comparison of the amino acid sequence...

Wild-type human p53 and a temperature-sensitive mutant induce Fas/APO-1 expression.

Owen-Schaub, L B, Zhang, W, Cusack, J C, Angelo, L S, Santee, S M, Fujiwara, T, ...

Fas/APO-1 is a cell surface protein known to trigger apoptosis upon specific antibody engagement. Because wild-type p53 can activate transcription as well as induce apoptosis, we queried whether p53...

Detection and characterization of a functional complex of human immunodeficiency virus type 1 integrase and its DNA substrate by UV cross-linking.

Yoshinaga, T, Kimura-Ohtani, Y, Fujiwara, T

To analyze the association of the human immunodeficiency virus type 1 integrase (IN) protein with DNA substrates, we applied a shortwave UV cross-linking method to the in vitro integration system....

Oral passive immunization against dental caries in rats by use of hen egg yolk antibodies specific for cell-associated glucosyltransferase of Streptococcus mutans.

Hamada, S, Horikoshi, T, Minami, T, Kawabata, S, Hiraoka, J, Fujiwara, T, ...

The effect of polyclonal egg yolk immunoglobulin G antibodies (yIgG) raised against whole cells, cell-free (CF) glucosyltransferase (GTase), or cell-associated (CA) GTase of serotype c Streptococcus...

Biochemical and immunobiological properties of lipopolysaccharide (LPS) from Bacteroides gingivalis and comparison with LPS from Escherichia coli.

Koga, T, Nishihara, T, Fujiwara, T, Nisizawa, T, Okahashi, N, Noguchi, T, ...

Lipopolysaccharides (LPSs) were isolated from Bacteroides gingivalis and Escherichia coli by the phenol-water and butanol-water procedures. The phenol-water-extracted LPS from B. gingivalis 381 was...

Chemical synthesis and serology of disaccharides and trisaccharides of phenolic glycolipid antigens from the leprosy bacillus and preparation of a disaccharide protein conjugate for serodiagnosis of leprosy.

Fujiwara, T, Hunter, S W, Cho, S N, Aspinall, G O, Brennan, P J

We examined the structural requirements within the species-specific 3,6-di-O-methyl-beta-D-glucopyranosyl-(1 leads to 4)-2,3-di-O-methyl- alpha-L-rhamnopyranosyl-(1 leads to...

Isolation of the Mycobacterium leprae-specific glycolipid antigen, phenolic glycolipid-I, from formalin-fixed human lepromatous liver.

Izumi, S, Sugiyama, K, Fujiwara, T, Hunter, S W, Brennan, P J

A Mycobacterium leprae-specific phenolic glycolipid antigen was purified from Formalin-fixed liver preserved from an advanced lepromatous leprosy patient. Its chemical and immunological properties...

Novel gelatin particle agglutination test for serodiagnosis of leprosy in the field.

Izumi, S, Fujiwara, T, Ikeda, M, Nishimura, Y, Sugiyama, K, Kawatsu, K

We developed a novel gelatin particle agglutination test (MLPA) for the serodiagnosis of leprosy; this test is especially useful for clinical practice and epidemiological surveys of leprosy in...

CDNA cloning of p112, the largest regulatory subunit of the human 26s proteasome, and functional analysis of its yeast homologue, sen3p.

Yokota, K, Kagawa, S, Shimizu, Y, Akioka, H, Tsurumi, C, Noda, C, ...

The 26S proteasome is a large multisubunit protease complex, the largest regulatory subunit of which is a component named p112. Molecular cloning of cDNA encoding human p112 revealed a polypeptide...

Microbial glycolipids: possible virulence factors that scavenge oxygen radicals.

Chan, J, Fujiwara, T, Brennan, P, McNeil, M, Turco, S J, Sibille, J C, ...

Two important pathogens of developing countries, Mycobacterium leprae, the etiologic agent of leprosy, and Leishmania donovani, the protozoal parasite that causes kalaazar, persist in the human host...

Integration of mini-retroviral DNA: a cell-free reaction for biochemical analysis of retroviral integration.

Fujiwara, T, Craigie, R

After retroviral infection of a permissive cell, the viral RNA is reverse-transcribed to make a DNA copy of the viral genome. Integration of this DNA copy into the host genome is a necessary step for...

Molecular design of a eukaryotic messenger RNA and its chemical synthesis.

Iwase, R, Maeda, M, Fujiwara, T, Sekine, M, Hata, T, Miura, K

A designed mRNA consisting of 42 ribonucleotides having the cap structure was synthesized. The capped leader sequence of the brome mosaic virus (BMV) mRNA 4, m7G5'pppGUAUUAAUA (F-1), was synthesized...

A novel guanine-guanine base pairing: crystal structure of a complex between 7-methylguanosine and its iodide.

Yamagata, Y, Fukumoto, S, Hamada, K, Fujiwara, T, Tomita, K

7-Methylguanosine, one of the biologically important minor nucleosides, could be crystallized as a complex of its zwitterionic form and its iodide, and the crystal structure was determined by the...

Reinvestigation of deoxyribonucleoside phosphorothioites: synthesis and properties of deoxyribonucleoside-3' dimethyl phosphites.

Nagai, H, Fujiwara, T, Fujii, M, Sekine, M, Hata, T

Further investigation of the synthesis of deoxyribonucleoside-3' (t-butyl) O-(2-cyanoethyl) phosphorothioites as monomer building units for the phosphorothioite approach has led us to conclude that...

Structural studies on the two forms of 8-bromo-2',3'-O-isopropylideneadenosine.

Fujii, S, Fujiwara, T, Tomita, K

The crystal and molecular structures of two forms of 8-bromo-2',3'-O-isopropylideneadenosine have been determined by X-ray methods. In one form, the molecular structure has planar conformation in the...

A downstream target of RHO1 small GTP-binding protein is PKC1, a homolog of protein kinase C, which leads to activation of the MAP kinase cascade in Saccharomyces cerevisiae.

Nonaka, H, Tanaka, K, Hirano, H, Fujiwara, T, Kohno, H, Umikawa, M, ...

The RHO1 gene in Saccharomyces cerevisiae encodes a homolog of the mammalian RhoA small GTP-binding protein, which is implicated in various actin cytoskeleton-dependent cell functions. In yeast,...

Bni1p implicated in cytoskeletal control is a putative target of Rho1p small GTP binding protein in Saccharomyces cerevisiae.

Kohno, H, Tanaka, K, Mino, A, Umikawa, M, Imamura, H, Fujiwara, T, ...

The RHO1 gene encodes a homolog of mammalian RhoA small GTP binding protein in the yeast Saccharomyces cerevisiae. Rho1p is localized at the growth sites, including the bud tip and the cytokinesis...

Troglitazone increases the number of small adipocytes without the change of white adipose tissue mass in obese Zucker rats.

Okuno, A, Tamemoto, H, Tobe, K, Ueki, K, Mori, Y, Iwamoto, K, ...

Troglitazone (CS-045) is one of the thiazolidinediones that activate the peroxisome proliferator-activated receptor gamma (PPARgamma), which is expressed primarily in adipose tissues. To elucidate...

Three-dimensional structure of fungal proteinase K reveals similarity to bacterial subtilisin.

Pähler, A, Banerjee, A, Dattagupta, J K, Fujiwara, T, Lindner, K, Pal, G P, ...

The three-dimensional structure of the fungal serine protease proteinase K has been determined at 3.3 A resolution by single crystal X-ray diffraction analysis. The enzyme crystallizes in the...

Brefeldin A arrests the intracellular transport of viral envelope proteins in primary cultured rat hepatocytes and HepG2 cells.

Oda, K, Fujiwara, T, Ikehara, Y

We have studied the effect of brefeldin A (BFA) on the intracellular transport of the envelope proteins of vesicular stomatitis virus (VSV) and sindbis virus in primary cultured rat hepatocytes. BFA...

X-ray diffraction studies of enkephalins. Crystal structure of [(4'-bromo) Phe4,Leu5]enkephalin.

Ishida, T, Kenmotsu, M, Mino, Y, Inoue, M, Fujiwara, T, Tomita, K, ...

In order to investigate the structure-activity relationship of [Leu5]- and [Met5]enkephalins, [(4'-bromo)Phe4, Leu5]-, [(4'-bromo)Phe4, Met5]- and [Met5] enkephalins were synthesized and...

Microbiological degradation of bile acids. The conjugation of a certain cholic acid metabolite with amino acids in Corynebacterium equi.

Hayakawa, S, Fujiwara, T, Kako, H

1. (4R)-4[4alpha-(2-Carboxyethyl)-3aalpha-hexahydro-7abeta-methyl-5-oxoindan-1beta-yl]valeric acid (II) could not be utilized by Arthrobacter simplex, even though the acid was one of the metabolites...

Microbiological degradation of bile acids, further degradation of a cholic acid metabolite containing the hexahydroindane nucleus by Corynebacterium equi.

Hayakawa, S, Fujiwara, T

1. The further degradation of a cholic acid (I) metabolite, (4R)-4-[4alpha-(2-carboxyethyl)-3aalpha-hexahydro-7abeta-methyl-5-oxoindan-1beta-yl]valeric acid (IIa), by Corynebacterium equi was...

Microbiological degradation of bile acids. The preparation of hexahydroindane derivatives as substrates for studying cholic acid degradation.

Hayakawa, S, Takata, T, Fujiwara, T, Hashimoto, S

Relatively large amounts of 3-(3aalpha-hexahydro-7abeta-methyl-1,5-dioxoindan-4alpha-yl)propionic acid (IIb), which is believed to be one of the intermediates involved in the degradation of cholic...

Microbiological degradation of bile acids. The preparation of some hypothetical metabolites involved in cholic acid degradation.

Hayakawa, S, Kanematsu, Y, Fujiwara, T, Kako, H

1. To identify the intermediates involved in the degradation of cholic acid, the further degradation of (4R)-4-[4alpha-(2-carboxyethyl)-3aalpha-hexahydro-7abeta-methyl-5-oxoindan-1beta-yl]valeric...

Effects of guanosine nucleotides on skinned smooth muscle tissue of the rabbit mesenteric artery.

Fujiwara, T, Itoh, T, Kubota, Y, Kuriyama, H

1. Effects of guanosine triphosphate (GTP) and guanosine 5'-o-(3-thio)triphosphate (GTP gamma S) on mechanical properties of skinned smooth muscle tissues of the rabbit mesenteric artery were...

Bip/GRP78 but not calnexin associates with a precursor of glycosylphosphatidylinositol-anchored protein.

Oda, K, Wada, I, Takami, N, Fujiwara, T, Misumi, Y, Ikehara, Y

When fused in-frame with a C-terminal propeptide of placental alkaline phosphatase (PLAP), rat alpha 2u-globulin (alpha GL), a nonglycosylated secretory protein, was expressed on the cell surface as...

Specific acceptance of fetal bowel allograft in mice after combined treatment with anti-intercellular adhesion molecule-1 and leukocyte function-associated antigen-1 antibodies.

Kato, Y, Yamataka, A, Yagita, H, Okumura, K, Fujiwara, T, Miyano, T

OBJECTIVE: The aim of this study was to see whether tolerance could be induced by simultaneous administration of monoclonal antibodies (MoAbs) to intercellular adhesion molecule-1 (ICAM-1) and...

Crystal structure combined with genetic analysis of the Thermus thermophilus ribosome recycling factor shows that a flexible hinge may act as a functional switch.

Toyoda, T, Tin, O F, Ito, K, Fujiwara, T, Kumasaka, T, Yamamoto, M, ...

Ribosome recycling factor (RRF), in concert with elongation factor EF-G, is required for disassembly of the posttermination complex of the ribosome after release of polypeptides. The crystal...

Functional mapping of ribosome-contact sites in the ribosome recycling factor: a structural view from a tRNA mimic.

Fujiwara, T, Ito, K, Nakamura, Y

Ribosome recycling factor (RRF) is required for disassembly of the posttermination complex of the ribosome after release of polypeptides. The crystal structure of RRF resembles a tRNA shape, with an...

Human vascular to cardiac tissue selectivity of L- and T-type calcium channel antagonists

Sarsero, D, Fujiwara, T, Molenaar, P, Angus, JA

1 Voltage-operated calcium channel (VOCC) antagonists are effective antihypertensive and antianginal agents but they also depress myocardial contractility. 2 We compared four L-type calcium channel...

High frequency of spontaneous acute myocardial infarction due to small coronary artery disease in dead (NZWxBXSB)F1 male mice.

Takemura, G., Fujiwara, H., Yoshida, H., Wu, D. J., Matsuda, M., Ishida, M., ...

To determine whether or not male (NZWXBXSB)F1 [WXB)F1) mice exhibiting a lupus-like syndrome died of acute myocardial infarction (MI), and whether acute MI is directly related to small coronary...

Quantitative analysis of vascular to cardiac selectivity of L- and T-type voltage-operated calcium channel antagonists in human tissues

Angus, J, Sarsero, D, Fujiwara, T, Molenaar, P, Xi, Q

1. Classical L-type voltage-operated calcium channel (VOCC) antagonists dilate blood vessels, depress myocardial contractility and slow cardiac conduction. 2. We compared four L-type VOCC antagonists...

Molecular therapy for peritoneal dissemination of xenotransplanted human MKN-45 gastric cancer cells with adenovirus mediated Bax gene transfer

Tsunemitsu, Y, Kagawa, S, Tokunaga, N, Otani, S, Umeoka, T, Roth, J A, ...

Background: Gene therapy is an innovative therapeutic approach for cancer. An adenoviral vector expressing the tumour suppressor p53 gene (Ad/p53) is currently under clinical evaluation for various...

Ventricular expression of atrial and brain natriuretic peptides in dilated cardiomyopathy. An immunohistocytochemical study of the endomyocardial biopsy specimens using specific monoclonal antibodies.

Hasegawa, K., Fujiwara, H., Doyama, K., Mukoyama, M., Nakao, K., Fujiwara, T., ...

Although brain natriuretic peptide is expressed in ventricles of failing hearts including dilated cardiomyopathy, its morphological localization is still unclear. In this study, we analyzed the...

Quantitative analysis of myocardial infarction in (NZW x BXSB)F1 hybrid mice with systemic lupus erythematosus and small coronary artery disease.

Yoshida, H., Fujiwara, H., Fujiwara, T., Ikehara, S., Hamashima, Y.

Male (NZW x BXSB)F1 mice ([W x B]F1) were used as a model for small coronary artery disease. The mortality rate for 162 mice was 0% at 12 weeks and 37% at 24 weeks. The incidence of myocardial...

Types of collagen synthesised by the lens epithelial cells of human cataracts.

Nishi, O, Nishi, K, Fujiwara, T, Shirasawa, E

AIMS/BACKGROUND--Residual lens epithelial cells (LECs) undergo fibrous proliferation after cataract surgery, resulting in capsular fibrosis. The purpose of this study was to determine the types of...

Effects of the cytokines on the proliferation of and collagen synthesis by human cataract lens epithelial cells.

Nishi, O, Nishi, K, Fujiwara, T, Shirasawa, E, Ohmoto, Y

AIMS: To assess the effects of the cytokines, interleukin-1 (IL-1), IL-1 receptor antagonist (IL-1ra), transforming growth factor-beta 2 (TGF-beta 2) and basic fibroblast growth factor (b-FGF), on...

Effect of immunoglobulin therapy on phagocytosis by polymorphonuclear leucocytes in whole blood of neonates

FUJIWARA, T, TANIUCHI, S, HATTORI, K, KOBAYASHI, T, KINOSHITA, Y, KOBAYASHI, Y

There has been some disagreement as to the clinical effect of intravenous immunoglobulin (IVIG) therapy on neonatal bacterial infections. We therefore evaluated the effect of IVIG therapy on neonatal...

Functional expression of Fas and Fas ligand on human intestinal intraepithelial lymphocytes

MORIMOTO, Y, HIZUTA, A, DING, E X, ISHII, T, HONGO, T, FUJIWARA, T, ...

Intestinal intraepithelial lymphocytes (IEL) constitute the first lymphoid compartment to encounter dietary antigens and intestinal pathogens. IEL are proposed to be involved in the defence against...

Relaxing actions of procaterol, a beta 2-adrenoceptor stimulant, on smooth muscle cells of the dog trachea.

Fujiwara, T., Sumimoto, K., Itoh, T., Suzuki, H., Kuriyama, H.

1. The effects of procaterol, a beta 2-adrenoceptor agonist, on smooth muscle cells of the dog trachea were investigated by use of microelectrode and isometric tension recording methods, and by...

Regional differences in the mechanical properties of rabbit airway smooth muscle.

Fujiwara, T., Itoh, T., Kuriyama, H.

1. In studies of rabbit airway smooth muscle, differences in mechanical responses to acetylcholine, histamine and high K+ in intact muscles, and in Ca2+ sensitivity in skinned muscles, have been...

Analysis of relaxation and repolarization mechanisms of nicorandil in rat mesenteric artery.

Fujiwara, T., Angus, J. A.

1. The mechanisms by which nicorandil causes relaxation of rat isolated small mesenteric arteries mounted on a Mulvany myograph was investigated by use of a combination of putatively...

Probing protein-cofactor interactions in the terminal oxidases by second derivative spectroscopy: study of bacterial enzymes with cofactor substitutions and heme A model compounds.

Felsch, J. S., Horvath, M. P., Gursky, S., Hobaugh, M. R., Goudreau, P. N., Fee, J. A., ...

Second derivative absorption spectra are reported for the aa3-cytochrome c oxidase from bovine cardiac mitochondria, the aa3-600 ubiquinol oxidase from Bacillus subtilis, the ba3-cytochrome c oxidase...

Idiopathic AA amyloidosis manifested by autonomic neuropathy, vestibulocochleopathy, and lattice corneal dystrophy.

Tsunoda, I, Awano, H, Kayama, H, Tsukamoto, T, Ueno, S, Fujiwara, T, ...

A 69-year-old Japanese woman with non-familial amyloidosis had polyneuropathy and profound autonomic neuropathy, and kappa chain monoclonal gammopathy. Immunohistopathological examination showed...

Localisation of a gene for mucopolysaccharidosis IIIC to the pericentromeric region of chromosome 8

Ausseil, J, Loredo-Osti, J, Verner, A, Darmond-Zwaig, C, Maire, I, Poorthuis, B, ...

Mucopolysaccharidosis type IIIC (MPS IIIC, or Sanfilippo syndrome C) is a rare lysosomal storage disorder caused by a deficiency of acetyl-coenzyme A:α-glucosaminide-N-acetyltransferase. Patients...

Poliomyelitis surveillance in Shandong Province, China, 1990-92.

Chiba, Y., Xu, A., Li, L., Lei, T., Takezaki, T., Hagiwara, A., ...

In Shandong Province, China, programmes were initiated in 1991 for mass immunization against poliomyelitis and for the immediate reporting of acute flaccid paralysis (AFP). The incidence of...

PET imaging of primary mediastinal tumours.

Kubota, K., Yamada, S., Kondo, T., Yamada, K., Fukuda, H., Fujiwara, T., ...

Mediastinal masses include a wide variety of tumours and remain an interesting diagnostic challenge for radiologist. We performed positron emission tomography (PET) studies of primary mediastinal...