T. Furuno

Hormone binding induces rapid proteasome-mediated degradation of thyroid hormone receptors

Dace, Alexandra, Zhao, Li, Park, Kyung Soo, Furuno, T., Takamura, N., Nakanishi, M., ...

The thyroid hormone 3,3′,5-triiodo-l-thyronine (T3) is essential for growth, differentiation, and development. Its biological activities are mediated by T3 nuclear receptors (TRs). At present, how...

Confocal fluorescence microscopy for studying thapsigargin-induced bivalent-cation entry into B cells.

Okamoto, Y, Furuno, T, Hamano, T, Nakanishi, M

We studied thapsigargin-induced bivalent-cation entry into antigen-specific B cells (TP67.21) with a confocal fluorescence microscope. Confocal fluorescence images of fluo-3-loaded B cells showed...

Thapsigargin-induced nuclear calcium signals in rat basophilic leukaemia cells.

Horikoshi, Y, Furuno, T, Teshima, R, Sawada, J, Nakanishi, M

By a confocal fluorescence microscope with an argon-ion laser (488 nm) and a He-Cd laser (325 nm) we have studied thapsigargin-induced calcium signals in individual rat basophilic leukaemia (RBL-2H3)...

Direct visualization of polypeptide shell of ferritin molecule by atomic force microscopy.

Ohnishi, S, Hara, M, Furuno, T, Okada, T, Sasabe, H

The polypeptide shell of the ferritin molecule has been imaged in water by atomic force microscopy (AFM). The central dip and the quaternary structure could be observed on the surface of the ferritin...

Two-dimensional crystallization of streptavidin by nonspecific binding to a surface film: study with a scanning electron microscope.

Furuno, T, Sasabe, H

A two-dimensional (2D) crystal of streptavidin has been obtained by a nonspecific binding method. The protein molecules were bound and formed a dense packing on the film of poly(1-benzyl-L-histidine)...

Receptor-mediated calcium signal playing a nuclear third messenger in the activation of antigen-specific B cells.

Furuno, T, Hamano, T, Nakanishi, M

We have studied receptor-mediated calcium signals in antigen-specific B cells (trinitrophenol-specific B cell clone, TP67.21) using a confocal fluorescence microscope with an argon ion laser (488 nm)...

Mechanical measurements of single actomyosin motor force.

Miyata, H, Yoshikawa, H, Hakozaki, H, Suzuki, N, Furuno, T, Ikegami, A, ...

To elucidate the mechanism of force generation by actomyosin motor, a measuring system was constructed, in which an in vitro motility assay was combined with an optical trapping technique. An actin...

Hormone binding induces rapid proteasome-mediated degradation of thyroid hormone receptors

Dace, Alexandra, Zhao, Li, Park, Kyung Soo, Furuno, T., Takamura, N., Nakanishi, M., ...

The thyroid hormone 3,3′,5-triiodo-l-thyronine (T3) is essential for growth, differentiation, and development. Its biological activities are mediated by T3 nuclear receptors (TRs). At present, how...

Confocal fluorescence microscopy for studying thapsigargin-induced bivalent-cation entry into B cells.

Okamoto, Y, Furuno, T, Hamano, T, Nakanishi, M

We studied thapsigargin-induced bivalent-cation entry into antigen-specific B cells (TP67.21) with a confocal fluorescence microscope. Confocal fluorescence images of fluo-3-loaded B cells showed...

Thapsigargin-induced nuclear calcium signals in rat basophilic leukaemia cells.

Horikoshi, Y, Furuno, T, Teshima, R, Sawada, J, Nakanishi, M

By a confocal fluorescence microscope with an argon-ion laser (488 nm) and a He-Cd laser (325 nm) we have studied thapsigargin-induced calcium signals in individual rat basophilic leukaemia (RBL-2H3)...

Direct visualization of polypeptide shell of ferritin molecule by atomic force microscopy.

Ohnishi, S, Hara, M, Furuno, T, Okada, T, Sasabe, H

The polypeptide shell of the ferritin molecule has been imaged in water by atomic force microscopy (AFM). The central dip and the quaternary structure could be observed on the surface of the ferritin...

Two-dimensional crystallization of streptavidin by nonspecific binding to a surface film: study with a scanning electron microscope.

Furuno, T, Sasabe, H

A two-dimensional (2D) crystal of streptavidin has been obtained by a nonspecific binding method. The protein molecules were bound and formed a dense packing on the film of poly(1-benzyl-L-histidine)...

Receptor-mediated calcium signal playing a nuclear third messenger in the activation of antigen-specific B cells.

Furuno, T, Hamano, T, Nakanishi, M

We have studied receptor-mediated calcium signals in antigen-specific B cells (trinitrophenol-specific B cell clone, TP67.21) using a confocal fluorescence microscope with an argon ion laser (488 nm)...

Mechanical measurements of single actomyosin motor force.

Miyata, H, Yoshikawa, H, Hakozaki, H, Suzuki, N, Furuno, T, Ikegami, A, ...

To elucidate the mechanism of force generation by actomyosin motor, a measuring system was constructed, in which an in vitro motility assay was combined with an optical trapping technique. An actin...

Low dose dobutamine stress echocardiography predicts the improvement of left ventricular systolic function in dilated cardiomyopathy

Kitaoka, H, Takata, J, Yabe, T, Hitomi, N, Furuno, T, Doi, Y

OBJECTIVE—To determine whether dobutamine stress echocardiography can predict the improvement of left ventricular systolic function in patients with dilated cardiomyopathy...

Occurrence of intimal thickening of the peripheral arteries in response to local vibration.

Okada, A, Inaba, R, Furuno, T

Experiments were performed to elucidate a possible mechanism for the peripheral circulatory disturbance caused by vibration. Rats were exposed to local vibration (60 Hz, 5 G) on their hind legs for...

4, 6-0-benzylidene-D-glucopyranose (BG) in the treatment of solid malignant tumours, an extended phase I study.

Tatsumura, T., Tsujimoto, M., Koyama, S., Furuno, T., Komori, Y., Sato, H., ...

4, 6-0-Benzylidene-D-glucopyranose (BG), a derivative of benzaldehyde (BA), whose anti-tumour action has often been reported, showed responses in 10 out of 24 patients (41.7%). These patients...