T. Hirose

Publication List Details

Period

1979 - 2009

Number

318

Co-Authors

The leaf anatomy of a broad-leaved evergreen allows an increase in leaf nitrogen content in winter (2009)

Muller, Onno, Oguchi, Riichi, Hirose, T., Werger, M.J.A., Hikosaka, K.

In temperate regions, evergreen species are exposed to large seasonal changes in air temperature and irradiance. They change photosynthetic characteristics of leaves responding to such environmental...

Dynamic Mechanical Properties of Reduced Activation Ferritic Steels (2008)

T. Hirose, H. Tanigawa, M. Ando, A. Kohyama, S. Jitsukawa

Abstract: A fatigue test method by a miniaturized hourglass-shaped fatigue specimen has been developed for International Fusion Materials Irradiation Facility (IFMIF) and sufficient potential as the...

Polarized positrons and electrons at the linear collider (2008)

Moortgat-Pick, G, Abe, T, Alexander, G, Ananthanarayan, B, Babich, AA, Bharadwaj, V, ...

The proposed International Linear Collider (ILC) is well-suited for discovering physics beyond the Standard Model and for precisely unraveling the structure of the underlying physics. The physics...

Light capture and plant architecture determine co-existence and competitive exclusion in grassland succession – and grazing interferes in this process (2008)

Werger, M.J.A., Hirose, T.

Plants evolved in dense stands and thus competition for light has always been a strong selective pressure. Here we show that the pattern of biomass partitioning of a plant strongly affects its light...

Pulsed neural networks consisting of single-flux-quantum spiking neurons (2007)

Hirose, T., Asai, T., Amemiya, Y.

An inhibitory pulsed neural network was developed for brain-like information processing, by using single-flux-quantum (SFQ) circuits. It consists of spiking neuron devices that are coupled to each...

CMOS Smart Sensor for Monitoring the Quality of Perishables (2007)

Ueno, K., Hirose, T., Asai, T., Amemiya, Y.

We developed a CMOS integrated-circuit sensor to monitor the change in quality of perishables that depends on surrounding temperatures. Our sensor makes use of the fact that the temperature...

CMOS Smart Sensor for Monitoring the Quality of Perishables (2007)

Ueno, K., Hirose, T., Asai, T., Amemiya, Y.

We developed a CMOS integrated-circuit sensor to monitor the change in quality of perishables that depends on surrounding temperatures. Our sensor makes use of the fact that the temperature...

Frictional Melt and Seismic Slip (2007)

Nielsen, S.; Istituto Nazionale Di Geofisica E Vulcanologia, Sezione Roma1, Roma, Italia, Di Toro, G.; Dipartimento Di Geoscienze, Università Di Padova, Hirose, T.; Kyoto University, Geology Department, Shimamoto, T.; Kyoto University, Geology Department

Abstract. Frictional melt is implied in a variety of processes such as seismic slip, ice skating and meteorite combustion. A steady-state can be reached when melt is continuously produced and...

Abstract Pulsed neural networks consisting of single-flux-quantum spiking neurons (2007)

T. Hirose, T. Asai, Y. Amemiya

An inhibitory pulsed neural network was developed for brain-like information processing, by using single-flux-quantum (SFQ) circuits. It consists of spiking neuron devices that are coupled to each...

Design of a Polarised Positron Source Based on Laser Compton Scattering (2006)

Araki, S., Higashi, Y., Honda, Y., Kurihara, Y., Kuriki, M., Okugi, T., ...

We describe a scheme for producing polarised positrons at the ILC from polarised X-rays created by Compton scattering of a few-GeV electron beam off a CO2 or YAG laser. This scheme is very energy...

CLIC Polarized Positron Source Based on Laser Compton Scattering (2006)

Zimmermann, Frank, Araki, S, Artru, X, Braun, H, Bulyak, E, Chehab, R, ...

We describe a possible layout and parameters of a polarized positron source for CLIC, where the positrons are produced from polarized gamma rays created by Compton scattering of a 1.3-GeV electron...

Design of a Polarised Positron Source Based on Laser Compton Scattering (2005)

Araki, S, Higashi, Y, Honda, Y, Kurihara, Y, Kuriki, M, Okugi, T, ...

We describe a scheme for producing polarised positrons at the ILC from polarised X-rays created by Compton scattering of a few-GeV electron beam off a CO2 or YAG laser. This scheme is very energy...

Design of a Polarised Positron Source Based on Laser Compton Scattering (2005)

Araki, S., Higashi, Y., Honda, Y., Kurihara, Y., Kuriki, M., Okugi, T., ...

We describe a scheme for producing polarised positrons at the ILC from polarised X-rays created by Compton scattering of a few-GeV electron beam off a CO2 or YAG laser. This scheme is very energy...

Efficient propagation of the polarization from laser photons to positrons through Compton scattering and electron-positron pair creation (2005)

Omori, T., Fukuda, M., Hirose, T., Kurihara, Y., Kuroda, R., Nomura, M., ...

We demonstrated for the first time the production of highly polarized short-pulse positrons with a finite energy spread in accordance with a new scheme that consists of two-quantum processes, such as...

The role of polarized positrons and electrons in revealing fundamental interactions at the Linear Collider (2005)

Moortgat-Pick, G, Abe, T, Alexander, G, Ananthanarayan, B, Babich, A A, Barber, D, ...

The proposed International Linear Collider (ILC) is well-suited for discovering physics beyond the Standard Model and for precisely unraveling the structure of the underlying physics. The physics...

Polarized positrons and electrons at the linear collider (2005)

Moortgat-Pick, G, Abe, T, Alexander, G, Ananthanarayan, B, Babich, A A, Barber, D, ...

The proposed International Linear Collider (ILC) is well-suited for discovering physics beyond the Standard Model and for precisely unraveling the structure of the underlying physics. The physics...

The role of polarized positrons and electrons in revealing fundamental interactions at the Linear Collider (2005)

Moortgat-Pick, G., Abe, T., Alexander, G., Ananthanarayan, B., Babich, A. A., Bharadwaj, V., ...

The proposed International Linear Collider (ILC) is well-suited for discovering physics beyond the Standard Model and for precisely unraveling the structure of the underlying physics. The physics...

Design of a Polarised Positron Source Based on Laser Compton Scattering (2005)

Araki, S., Higashi, Y., Honda, Y., Kurihara, Y., Kuriki, M., Okugi, T., ...

We describe a scheme for producing polarised positrons at the ILC from polarised X-rays created by Compton scattering of a few-GeV electron beam off a CO2 or YAG laser. This scheme is very energy...

Design of a Polarised Positron Source Based on Laser Compton Scattering (2005)

Araki, S., Higashi, Y., Honda, Y., Kurihara, Y., Kuriki, M., Okugi, T., ...

We describe a scheme for producing polarised positrons at the ILC from polarised X-rays created by Compton scattering of a few-GeV electron beam off a CO2 or YAG laser. This scheme is very energy...

Preface: Structure and Function of Plant Canopies (2005)

HIROSE, T., TERASHIMA, I.

This section comprises a set of papers taken from those presented at a symposium held to commemorate the 50th anniversary of the Monsi–Saeki theory (1953), together with invited papers. The papers...

Structure and Function of Plant Canopies (2004)

HIROSE, T., TERASHIMA, I.

This section comprises a set of papers taken from those presented at a symposium held to commemorate the 50th anniversary of the Monsi-Saeki theory (1953), together with invited papers. The papers...

Structure and Function of Plant Canopies (2004)

HIROSE, T., TERASHIMA, I.

This section comprises a set of papers taken from those presented at a symposium held to commemorate the 50th anniversary of the Monsi-Saeki theory (1953), together with invited papers. The papers...

Achievement of ultra-low emittance beam in the ATF damping ring (2003)

Honda, Y, Anderson, S, Araki, S, Bane, Karl Leopold Freitag, Brachmann, A, Frisch, J, ...

We report on the smallest vertical emittance achieved in single-bunch-mode operation of the ATF. The emittances were measured with a laser-wire beam-profile monitor installed in the damping ring. The...

Shoot structure,leaf physiology, and daily carbon gain of plant species in a tallgrass meadow (2003)

Anten, N.P.R., Hirose, T.

We analyzed the importance of shoot structural and leaf physiological characteristics in determining interspecific differences in whole-shoot carbon gain in a tallgrass meadow. Canopy structure,...

Muscle fibre composition and electromyographic features of cervical muscles following prolonged head extension in growing rats (2003)

Yoshida, R., Hirose, T., Ito, G.

Soft tissue stretching has been proposed as one of the control factors in craniofacial morphogenesis. However, its mechanism remains unclear. The present study investigated electromyographic (EMG)...

Trade-off Between Light- and Nitrogen-use Efficiency in Canopy Photosynthesis (1998)

HIROSE, T., BAZZAZ, F. A.

If the light-use efficiency (LUE) of species in a canopy is constant, canopy photosynthesis (CP) is proportional to the number of photons (Φ) absorbed by the canopy (CP=LUE×Φ). Likewise, if...

Osteoporosis-pseudoglioma syndrome, a disorder affecting skeletal strength and vision, is assigned to chromosome region 11q12-13. (1996)

Gong, Y., Vikkula, M., Boon, L., Liu, J., Beighton, P., Ramesar, R., ...

Osteoporosis-pseudoglioma syndrome (OPS) is an autosomal recessive disorder characterized by severe juvenile-onset osteoporosis and congenital or juvenile-onset blindness. The pathogenic mechanism is...

Exclusive $\varrho^{0}$ production in deep inelastic electron-proton scattering at HERA (1995)

Derrick, Malcolm, Krakauer, D A, Magill, S, Mikunas, D, Musgrave, B, Repond, J, ...

Inclusive jet cross sections for events with a large rapidity gap with respect to the proton direction from the reaction ep \rightarrow jet \; + \; X with quasi-real photons have been measured with...

Nitrogen Uptake and Plant Growth I. Effect of Nitrogen Removal on Growth of Polygonum cuspidatum (1986)

HIROSE, T., KITAJIMA, K.

Polygonun cuspidatum was grown hydroponically to examine the effect of nitrogen removal from the nutrient solution upon plant growth and the partitioning of dry matter and nitrogen among organs....

Nitrogen Uptake and Plant Growth II. An Empirical Model of Vegetative Growth and Partitioning (1986)

HIROSE, T.

An empirical model of vegetative plant growth is presented. The model is based on experimental data on Polygonum cuspidatum, which showed (1) that the partitioning of dry matter and nitrogen among...

Nitrogen Use Efficiency in Growth of Polygonum cuspidatum Sieb. et Zucc (1984)

HIROSE, T.

The growth of Polygonum cuspidatum in sand culture was analysed under varying nutrient conditions. Nitrogen availability influenced nitrogen uptake of plants through the uptake rate per unit root...

Hybridization of synthetic oligodeoxyribonucleotides to ΦX 174 DNA: the effect of single base pair mismatch (1979)

Wallace, R. Bruce, Shaffer, J., Murphy, R. F., Bonner, J., Hirose, T., Itakura, K.

Oligodeoxyribonucleotides complementary to the DNA of the wild type (wt) bacteriophage ΦX174 have been synthesized by the phosphotriester method. The ollgomers, 11, 14, and 17 bases long, are...

Hybridization of synthetic oligodeoxyribonucleotides to {Phi}X 174 DNA: the effect of single base pair mismatch (1979)

Wallace, R. Bruce, Shaffer, J., Murphy, R.F., Bonner, J., Hirose, T., Itakura, K.

Oligodeoxyribonucleotides complementary to the DNA of the wild type (wt) bacteriophage ΦX174 have been synthesized by the phosphotriester method. The ollgomers, 11, 14, and 17 bases long, are...

Creation of a novel protein-coding region at the RNA level in black pine chloroplasts: the pattern of RNA editing in the gymnosperm chloroplast is different from that in angiosperms.

Wakasugi, T, Hirose, T, Horihata, M, Tsudzuki, T, Kössel, H, Sugiura, M

The phenomenon of RNA editing has been found to occur in chloroplasts of several angiosperm plants. Comparative analysis of the entire nucleotide sequence of a gymnosperm [Pinus thunbergii (black...

Structure-function relationship of human neutrophil collagenase: identification of regions responsible for substrate specificity and general proteinase activity.

Hirose, T, Patterson, C, Pourmotabbed, T, Mainardi, C L, Hasty, K A

The family of matrix metalloproteinases is a family of closely related enzymes that play an important role in physiological and pathological processes of matrix degradation. The most distinctive...

Automated Multiplex Assay System for Simultaneous Detection of Hepatitis B Virus DNA, Hepatitis C Virus RNA, and Human Immunodeficiency Virus Type 1 RNA

Meng, Q., Wong, C., Rangachari, A., Tamatsukuri, S., Sasaki, M., Fiss, E., ...

We have developed an automated multiplex system for simultaneously screening hepatitis B virus (HBV), hepatitis C virus (HCV), and human immunodeficiency virus type 1 (HIV-1) in blood donations. The...

Purification and some properties of sulfite:ferric ion oxidoreductase from Thiobacillus ferrooxidans.

Sugio, T, Hirose, T, Ye, L Z, Tano, T

Sulfite:ferric ion oxidoreductase in the plasma membrane of Thiobacillus ferrooxidans AP19-3 was purified to an electrophoretically homogeneous state. The enzyme had an apparent molecular weight of...

Pharmacokinetics of ceftizoxime in animals after parenteral dosing.

Murakawa, T, Sakamoto, H, Fukada, S, Nakamoto, S, Hirose, T, Itoh, N, ...

The pharmacokinetic profile of ceftizoxime was studied in mice, rats, dogs, and monkeys given the drug in a single parenteral dose. The serum data after an intravenous injection were analyzed by the...

New in vitro kinetic model for evaluating bactericidal efficacy of antibiotics.

Murakawa, T, Sakamoto, H, Hirose, T, Nishida, M

A new in vitro model was devised for evaluating the bactericidal activity of antibiotics under dynamic conditions. This model accurately reproduced the observed serum levels of antibiotics after...

In vitro and in vivo activity of NY-198, a new difluorinated quinolone.

Hirose, T, Okezaki, E, Kato, H, Ito, Y, Inoue, M, Mitsuhashi, S

NY-198 [1-ethyl-6,8-difluoro-1,4-dihydro-7-(3-methyl-1-piperazinyl)-4-oxo-3- quinolinecarboxylic acid hydrochloride] is a new difluorinated quinolone characterized by the presence of a C-methyl group...

cDNA structure, expression and nucleic acid-binding properties of three RNA-binding proteins in tobacco: occurrence of tissue-specific alternative splicing.

Hirose, T, Sugita, M, Sugiura, M

Three cDNAs encoding RNA-binding proteins were isolated from a tobacco (Nicotiana sylvestris) cDNA library. The predicted proteins (RGP-1) are homologous to each other and consist of a...

Isolation and characterization of bovine and mouse terminal deoxynucleotidyltransferase cDNAs expressible in mammalian cells.

Koiwai, O, Yokota, T, Kageyama, T, Hirose, T, Yoshida, S, Arai, K

We have isolated nearly full-length cDNA clones of terminal deoxynucleotidyltransferase (TdT) from calf thymus and mouse lymphoma cDNA libraries. The libraries were constructed using the pcD vector...

The use of synthetic oligonucleotides as hybridization probes. II. Hybridization of oligonucleotides of mixed sequence to rabbit beta-globin DNA.

Wallace, R B, Johnson, M J, Hirose, T, Miyake, T, Kawashima, E H, Itakura, K

Two oligonucleotides 14-bases long were synthesized, one complementary to rabbit beta-globin DNA (R beta G14A) and the other with the same sequence except for a single base change (T for C) (R beta...

Hybridization of synthetic oligodeoxyribonucleotides to phi chi 174 DNA: the effect of single base pair mismatch.

Wallace, R B, Shaffer, J, Murphy, R F, Bonner, J, Hirose, T, Itakura, K

Oligodeoxyribonucleotides complementary to the DNA of the wild type (wt) bacteriophage phi chi 174 have been synthesized by the phosphotriester method. The oligomers, 11, 14, and 17 bases long, are...

Efficient correction of a mutation by use of chemically synthesized DNA.

Razin, A, Hirose, T, Itakura, K, Riggs, A D

The mutated base in the am3 lysis-defective mutant of the bacteriophage phiX174 has been corrected by a combined in vitro enzymatic DNA synthesis and in vivo replication of the heteroduplex product....

Analysis of the complete nucleotide sequence of the group IV RNA coliphage SP.

Inokuchi, Y, Jacobson, A B, Hirose, T, Inayama, S, Hirashima, A

We report the nucleotide sequence of the Group IV RNA bacteriophage SP. The entire sequence is 4276 nucleotides long. Four cistrons have been identified by comparison with the related Group III phage...

Contact points between transcription machinery and the fibroin gene promoter deduced by functional tests of single-base substitution mutants.

Hirose, S, Takeuchi, K, Hori, H, Hirose, T, Inayama, S, Suzuki, Y

An efficient method for oligonucleotide-directed mutagenesis was developed to construct a set of site-specific mutations by using a mixture of oligodeoxyribonucleotides. With this method, as high as...

Use of synthetic oligonucleotides as hybridization probes: isolation of cloned cDNA sequences for human beta 2-microglobulin.

Suggs, S V, Wallace, R B, Hirose, T, Kawashima, E H, Itakura, K

We have synthesized two sets of 15-base-long oligodeoxyribonucleotides corresponding to all possible coding sequences for a small portion of human beta 2-microglobulin. Labeled oligonucleotides were...

Expression in Escherichia coli of chemically synthesized genes for human insulin.

Goeddel, D V, Kleid, D G, Bolivar, F, Heyneker, H L, Yansura, D G, Crea, R, ...

Synthetic genes for human insulin A and B chains were cloned separately in plasmid pBR322. The cloned synthetic genes were then fused to an Escherichia coli beta-galactosidase gene to provide...

Molecular cloning and nucleotide sequence of cDNA for mRNA of mitochondrial cytochrome P-450(SCC) of bovine adrenal cortex.

Morohashi, K, Fujii-Kuriyama, Y, Okada, Y, Sogawa, K, Hirose, T, Inayama, S, ...

We have isolated cDNA clones of the mRNA for cytochrome P-450(SCC), which catalyzes the side-chain cleavage reaction of cholesterol in bovine adrenal cortex mitochondria, by using synthetic...

Chemical synthesis of genes for human insulin.

Crea, R, Kraszewski, A, Hirose, T, Itakura, K

A rapid chemical procedure has been developed and used for the synthesis of 29 oligodeoxyribonucleotides to build synthetic genes for human insulin. The gene for insulin B chain, 104 base pairs, and...

Primary structures of bovine liver low molecular weight kininogen precursors and their two mRNAs.

Nawa, H, Kitamura, N, Hirose, T, Asai, M, Inayama, S, Nakanishi, S

By using a mixture of synthetic oligodeoxyribonucleotides as a probe, cloned cDNA sequences specific for low molecular weight (LMW) kininogen have been isolated from a cDNA library of bovine liver...

Cloning and sequence analysis of cDNA for rat angiotensinogen.

Ohkubo, H, Kageyama, R, Ujihara, M, Hirose, T, Inayama, S, Nakanishi, S

A mixture of tetradecamer oligodeoxyribonucleotides complementary to the codons specifying the carboxyl-terminal sequence, Ile-His-Pro-Phe-His, of angiotensin was chemically synthesized as two pools...

Cis-acting elements and trans-acting factors for accurate translation of chloroplast psbA mRNAs: development of an in vitro translation system from tobacco chloroplasts.

Hirose, T, Sugiura, M

Translational regulation is an important step of gene expression in chloroplasts. To analyze biochemical mechanisms of translational regulation unique to higher plant chloroplasts, an in vitro...

Both RNA editing and RNA cleavage are required for translation of tobacco chloroplast ndhD mRNA: a possible regulatory mechanism for the expression of a chloroplast operon consisting of functionally unrelated genes.

Hirose, T, Sugiura, M

Tobacco chloroplast genes encoding a photosystem I component (psaC) and a NADH dehydrogenase subunit (ndhD) are transcribed as a dicistronic pre-mRNA which is then cleaved into short mRNAs. An RNA...

Early response gene signalling in bryostatin-stimulated primary B chronic lymphocytic leukaemia cells in vitro.

Ning, Z Q, Hirose, T, Deed, R, Newton, J, Murphy, J J, Norton, J D

The protein kinase C activator bryostatin induces differentiation and antagonizes the effects of tumour-promoting phorbol esters in a number of different cell types. We show here that bryostatin...

Genomic organization of the S locus: Identification and characterization of genes in SLG/SRK region of S(9) haplotype of Brassica campestris (syn. rapa).

Suzuki, G, Kai, N, Hirose, T, Fukui, K, Nishio, T, Takayama, S, ...

In Brassica, two self-incompatibility genes, encoding SLG (S locus glycoprotein) and SRK (S-receptor kinase), are located at the S locus and expressed in the stigma. Recent molecular analysis has...

Creation of a novel protein-coding region at the RNA level in black pine chloroplasts: the pattern of RNA editing in the gymnosperm chloroplast is different from that in angiosperms.

Wakasugi, T, Hirose, T, Horihata, M, Tsudzuki, T, Kössel, H, Sugiura, M

The phenomenon of RNA editing has been found to occur in chloroplasts of several angiosperm plants. Comparative analysis of the entire nucleotide sequence of a gymnosperm [Pinus thunbergii (black...

Structure-function relationship of human neutrophil collagenase: identification of regions responsible for substrate specificity and general proteinase activity.

Hirose, T, Patterson, C, Pourmotabbed, T, Mainardi, C L, Hasty, K A

The family of matrix metalloproteinases is a family of closely related enzymes that play an important role in physiological and pathological processes of matrix degradation. The most distinctive...

Automated Multiplex Assay System for Simultaneous Detection of Hepatitis B Virus DNA, Hepatitis C Virus RNA, and Human Immunodeficiency Virus Type 1 RNA

Meng, Q., Wong, C., Rangachari, A., Tamatsukuri, S., Sasaki, M., Fiss, E., ...

We have developed an automated multiplex system for simultaneously screening hepatitis B virus (HBV), hepatitis C virus (HCV), and human immunodeficiency virus type 1 (HIV-1) in blood donations. The...

Purification and some properties of sulfite:ferric ion oxidoreductase from Thiobacillus ferrooxidans.

Sugio, T, Hirose, T, Ye, L Z, Tano, T

Sulfite:ferric ion oxidoreductase in the plasma membrane of Thiobacillus ferrooxidans AP19-3 was purified to an electrophoretically homogeneous state. The enzyme had an apparent molecular weight of...

Pharmacokinetics of ceftizoxime in animals after parenteral dosing.

Murakawa, T, Sakamoto, H, Fukada, S, Nakamoto, S, Hirose, T, Itoh, N, ...

The pharmacokinetic profile of ceftizoxime was studied in mice, rats, dogs, and monkeys given the drug in a single parenteral dose. The serum data after an intravenous injection were analyzed by the...

New in vitro kinetic model for evaluating bactericidal efficacy of antibiotics.

Murakawa, T, Sakamoto, H, Hirose, T, Nishida, M

A new in vitro model was devised for evaluating the bactericidal activity of antibiotics under dynamic conditions. This model accurately reproduced the observed serum levels of antibiotics after...

In vitro and in vivo activity of NY-198, a new difluorinated quinolone.

Hirose, T, Okezaki, E, Kato, H, Ito, Y, Inoue, M, Mitsuhashi, S

NY-198 [1-ethyl-6,8-difluoro-1,4-dihydro-7-(3-methyl-1-piperazinyl)-4-oxo-3- quinolinecarboxylic acid hydrochloride] is a new difluorinated quinolone characterized by the presence of a C-methyl group...

cDNA structure, expression and nucleic acid-binding properties of three RNA-binding proteins in tobacco: occurrence of tissue-specific alternative splicing.

Hirose, T, Sugita, M, Sugiura, M

Three cDNAs encoding RNA-binding proteins were isolated from a tobacco (Nicotiana sylvestris) cDNA library. The predicted proteins (RGP-1) are homologous to each other and consist of a...

Isolation and characterization of bovine and mouse terminal deoxynucleotidyltransferase cDNAs expressible in mammalian cells.

Koiwai, O, Yokota, T, Kageyama, T, Hirose, T, Yoshida, S, Arai, K

We have isolated nearly full-length cDNA clones of terminal deoxynucleotidyltransferase (TdT) from calf thymus and mouse lymphoma cDNA libraries. The libraries were constructed using the pcD vector...

The use of synthetic oligonucleotides as hybridization probes. II. Hybridization of oligonucleotides of mixed sequence to rabbit beta-globin DNA.

Wallace, R B, Johnson, M J, Hirose, T, Miyake, T, Kawashima, E H, Itakura, K

Two oligonucleotides 14-bases long were synthesized, one complementary to rabbit beta-globin DNA (R beta G14A) and the other with the same sequence except for a single base change (T for C) (R beta...

Hybridization of synthetic oligodeoxyribonucleotides to phi chi 174 DNA: the effect of single base pair mismatch.

Wallace, R B, Shaffer, J, Murphy, R F, Bonner, J, Hirose, T, Itakura, K

Oligodeoxyribonucleotides complementary to the DNA of the wild type (wt) bacteriophage phi chi 174 have been synthesized by the phosphotriester method. The oligomers, 11, 14, and 17 bases long, are...

Efficient correction of a mutation by use of chemically synthesized DNA.

Razin, A, Hirose, T, Itakura, K, Riggs, A D

The mutated base in the am3 lysis-defective mutant of the bacteriophage phiX174 has been corrected by a combined in vitro enzymatic DNA synthesis and in vivo replication of the heteroduplex product....

Analysis of the complete nucleotide sequence of the group IV RNA coliphage SP.

Inokuchi, Y, Jacobson, A B, Hirose, T, Inayama, S, Hirashima, A

We report the nucleotide sequence of the Group IV RNA bacteriophage SP. The entire sequence is 4276 nucleotides long. Four cistrons have been identified by comparison with the related Group III phage...

Contact points between transcription machinery and the fibroin gene promoter deduced by functional tests of single-base substitution mutants.

Hirose, S, Takeuchi, K, Hori, H, Hirose, T, Inayama, S, Suzuki, Y

An efficient method for oligonucleotide-directed mutagenesis was developed to construct a set of site-specific mutations by using a mixture of oligodeoxyribonucleotides. With this method, as high as...

Use of synthetic oligonucleotides as hybridization probes: isolation of cloned cDNA sequences for human beta 2-microglobulin.

Suggs, S V, Wallace, R B, Hirose, T, Kawashima, E H, Itakura, K

We have synthesized two sets of 15-base-long oligodeoxyribonucleotides corresponding to all possible coding sequences for a small portion of human beta 2-microglobulin. Labeled oligonucleotides were...

Expression in Escherichia coli of chemically synthesized genes for human insulin.

Goeddel, D V, Kleid, D G, Bolivar, F, Heyneker, H L, Yansura, D G, Crea, R, ...

Synthetic genes for human insulin A and B chains were cloned separately in plasmid pBR322. The cloned synthetic genes were then fused to an Escherichia coli beta-galactosidase gene to provide...

Molecular cloning and nucleotide sequence of cDNA for mRNA of mitochondrial cytochrome P-450(SCC) of bovine adrenal cortex.

Morohashi, K, Fujii-Kuriyama, Y, Okada, Y, Sogawa, K, Hirose, T, Inayama, S, ...

We have isolated cDNA clones of the mRNA for cytochrome P-450(SCC), which catalyzes the side-chain cleavage reaction of cholesterol in bovine adrenal cortex mitochondria, by using synthetic...

Chemical synthesis of genes for human insulin.

Crea, R, Kraszewski, A, Hirose, T, Itakura, K

A rapid chemical procedure has been developed and used for the synthesis of 29 oligodeoxyribonucleotides to build synthetic genes for human insulin. The gene for insulin B chain, 104 base pairs, and...

Primary structures of bovine liver low molecular weight kininogen precursors and their two mRNAs.

Nawa, H, Kitamura, N, Hirose, T, Asai, M, Inayama, S, Nakanishi, S

By using a mixture of synthetic oligodeoxyribonucleotides as a probe, cloned cDNA sequences specific for low molecular weight (LMW) kininogen have been isolated from a cDNA library of bovine liver...

Cloning and sequence analysis of cDNA for rat angiotensinogen.

Ohkubo, H, Kageyama, R, Ujihara, M, Hirose, T, Inayama, S, Nakanishi, S

A mixture of tetradecamer oligodeoxyribonucleotides complementary to the codons specifying the carboxyl-terminal sequence, Ile-His-Pro-Phe-His, of angiotensin was chemically synthesized as two pools...

Cis-acting elements and trans-acting factors for accurate translation of chloroplast psbA mRNAs: development of an in vitro translation system from tobacco chloroplasts.

Hirose, T, Sugiura, M

Translational regulation is an important step of gene expression in chloroplasts. To analyze biochemical mechanisms of translational regulation unique to higher plant chloroplasts, an in vitro...

Both RNA editing and RNA cleavage are required for translation of tobacco chloroplast ndhD mRNA: a possible regulatory mechanism for the expression of a chloroplast operon consisting of functionally unrelated genes.

Hirose, T, Sugiura, M

Tobacco chloroplast genes encoding a photosystem I component (psaC) and a NADH dehydrogenase subunit (ndhD) are transcribed as a dicistronic pre-mRNA which is then cleaved into short mRNAs. An RNA...

Early response gene signalling in bryostatin-stimulated primary B chronic lymphocytic leukaemia cells in vitro.

Ning, Z Q, Hirose, T, Deed, R, Newton, J, Murphy, J J, Norton, J D

The protein kinase C activator bryostatin induces differentiation and antagonizes the effects of tumour-promoting phorbol esters in a number of different cell types. We show here that bryostatin...

Genomic organization of the S locus: Identification and characterization of genes in SLG/SRK region of S(9) haplotype of Brassica campestris (syn. rapa).

Suzuki, G, Kai, N, Hirose, T, Fukui, K, Nishio, T, Takayama, S, ...

In Brassica, two self-incompatibility genes, encoding SLG (S locus glycoprotein) and SRK (S-receptor kinase), are located at the S locus and expressed in the stigma. Recent molecular analysis has...

Electroretinogram b/a wave ratio improvement in central retinal vein obstruction.

Matsui, Y, Katsumi, O, Sakaue, H, Hirose, T

The electroretinogram (ERG), especially the b/a wave ratio, is considered a good indicator of retinal ischaemia in central retinal vein obstruction (CRVO). Seven CRVO patients who showed b/a wave...

Objective measurement of contrast sensitivity function using contrast sweep visual evoked responses

Katsumi, O., Arai, M., Hirose, T.

AIM/BACKGROUND—The contrast sensitivity function (CSF) measurement provides information that is not accessible by standard visual acuity determinations. The contrast sweep pattern reversal visual...

Expression of smooth muscle markers in so called malignant fibrous histiocytomas

Hasegawa, T, Hasegawa, F, Hirose, T, Sano, T, Matsuno, Y

Aims: To obtain further information regarding the frequency and degree of positivity for smooth muscle markers in a large number of malignant fibrous histiocytomas (MFHs), as an aid to accurate...

Low grade malignant peripheral nerve sheath tumour: varied cytological and histological patterns

Yamaguchi, U, Hasegawa, T, Hirose, T, Chuman, H, Kawai, A, Ito, Y, ...

Background: A small number of malignant peripheral nerve sheath tumours (MPNSTs) are low grade, and the nature of these low grade tumours has never been systematically assessed.

Heterogeneity of B cell responsiveness to interleukin 4, interleukin 6 and low molecular weight B cell growth factor in discrete stages of B cell activation in patients with systemic lupus erythematosus.

Kitani, A, Hara, M, Hirose, T, Norioka, K, Harigai, M, Hirose, W, ...

To investigate the differential stages of B cell activation in patients with systemic lupus erythematosus (SLE), the effects of recombinant interleukin-4 (rIL-4) interleukin-6 (rIL-6), and low mol....

Production of thymocyte-stimulating activity by cultured human thyroid epithelial cells.

Hirose, W, Kawagoe, M, Hara, M, Kitani, A, Hirose, T, Norioka, K, ...

Since thyroid follicular epithelial cells (thyrocytes) have been shown to express a number of functions similar to monocytes, they were further examined for their potency in secreting...

Autostimulatory effects of IL-6 on excessive B cell differentiation in patients with systemic lupus erythematosus: analysis of IL-6 production and IL-6R expression.

Kitani, A, Hara, M, Hirose, T, Harigai, M, Suzuki, K, Kawakami, M, ...

Introducing avidin-biotin complex ELISA for anti-DNA antibody, the mechanism of in vitro production of anti-ssDNA antibody as well as of polyclonal immunoglobulin mediated by an IL-6-IL-6R loop was...

Osteoporosis-pseudoglioma syndrome, a disorder affecting skeletal strength and vision, is assigned to chromosome region 11q12-13.

Gong, Y., Vikkula, M., Boon, L., Liu, J., Beighton, P., Ramesar, R., ...

Osteoporosis-pseudoglioma syndrome (OPS) is an autosomal recessive disorder characterized by severe juvenile-onset osteoporosis and congenital or juvenile-onset blindness. The pathogenic mechanism is...

The spontaneous electrical and mechanical activity of human bronchial smooth muscle: its modulation by drugs.

Ito, Y., Suzuki, H., Aizawa, H., Hakoda, H., Hirose, T.

1. Tissue taken at operation was used to study the electrical and mechanical properties of human bronchial smooth muscle with intracellular microelectrodes and isometric recording of tension changes....

Effects of vasoactive intestinal polypeptide antagonists on cholinergic neurotransmission in dog and cat trachea.

Xie, Z. Q., Hirose, T., Hakoda, H., Ito, Y.

1. The effects of vasoactive intestinal polypeptide (VIP) antagonists [AC-Tyr1, D-Phe2]-GRF(1-29)-NH2 and [4-Cl-D-Phe6, Leu17]-VIP on excitatory neuroeffector transmission in the dog and cat trachea...

保存血輸血の經驗について

田中, 康雄, 藤森, 英雄, 廣瀨, 輝夫, 高村, 良平, 大西, 盛光, 山本, 勝美, ...

We have experienced in 50 cases, the preserved blood trnasfusion by our own method. The blood is 1 to 7 days, and the transfusion quantity is 400cc on the average. Their secondary reaction is 6%, but...