T. Honda

Publication List Details

Period

1980 - 2009

Number

198

Co-Authors

Directional Voting with Geometric Hashing for Three Dimensional Object Recognition (2009)

S. Kaneko, M. Shibata, T. Honda

the combinations of triplets of basis points from the set of feature points and then the HT can include normalized A new method for recognition of three dimensional information of feature...

Development of 40 inch hybrid hologram screen for auto stereoscopic video display (2008)

Hyun Ho. Song, Y. Nakashima, Y. Momonoi, T. Honda, Manku Dowhadong Incheon

Usually in auto stereoscopic display, there are two problems. The first problem is that large image display is difficult, and the second problem is that the view zone (which means the zone in which...

INVESTIGATION AND DEVELOPMENT OF REDUNDANCY TECHNIQUES TO ACHIEVE DUAL FAULT CORRECTIVE CAPABILITY IN FLIGHT CONTROL ACTUATORS. (2005)

Rinde, J. E., Coppola, P. S., Musser, E., Wildrick, M., Honda, T.

The objective of this study and investigation was to develop dual fault corrective capability for increased reliability by applying redundancy at the element level in flight control actuation...

Effect of periodontal treatment on the C-reactive protein and proinflammatory cytokine levels in Japanese periodontitis patients (2005)

Yamazaki, K, Honda, T, Oda, T, Ueki-Maruyama, K, Nakajima, T, Yoshie, H, ...

Background: Recent epidemiological studies have shown that individuals with periodontitis have a significantly increased risk of developing coronary heart disease. In addition to conventional risk...

Development of a simple method to evaluate medical staff radiation dose and its application to a software system supporting PET facility operation (2005)

Hayashi, K., Tayama, R., Shibata, K., Honda, T., Morimoto, M., Izumida, T., ...

In recent years, positron-emitting labelled radiopharmaceuticals have come to be used in conjunction with positron emission tomography (PET) in various clinical diagnoses. Radiation exposure of the...

Dendritic side-branching with anisotropic viscous fingering (2004)

Honda, T., Honjo, H., Katsuragi, H.

We studied dendritic side-branching mechanism in the experiment of anisotropic viscous fingering. We measured the time dependence of growth speed of side-branch and the envelop of side-branches. We...

Evidence for high specificity and efficiency of multiple recombination signals in mixed DNA cloning by the Multisite Gateway system (2004)

Sone, T, Yoshida, S, Yahata, K, Chesnut, JD, ...

Six types of recombination signal DNA sequences of the Multisite Gateway cloning system were investigated as to their specificity and efficiency in the LR and BP recombination reactions. In the LR...

Effect of periodontal treatment on the serum antibody levels to heat shock proteins (2004)

Yamazaki, K, Ueki-Maruayama, K, Honda, T, Nakajima, T, Seymour, GJ

We have shown previously that both humoral and cellular immune responses to heat shock protein 60 (HSP60) are elevated in chronic periodontitis patients compared with non-diseased subjects. The aim...

Quenching of neutron E2 effective charge in neutron-rich nuclei and the ground-state spin-parity of C-17 (2002)

Asahi, K, Ueno, H, Sakai, H, Miyoshi, H, Kameda, D, ...

The electric quadrupole moment of B-17 and the g-factor of C-17 were measured by using the fragmentation-induced nuclear polarization technique combined with the beta-NMR method. The experimental...

Stable Chromosome Aberrations in Atomic Bomb Survivors: Results from 25 Years of Investigation (2001)

Y. Kodama, D. Pawel, N. Nakamura, D. Preston, T. Honda, M. Itoh, ...

Kodama, Y., Pawel, D., Nakamura, N., Preston, D., Honda, T., Itoh, M., Nakano, M., Ohtaki, K., Funamoto, S. and Awa, A. A. Stable Chromosome Aberrations in Atomic Bomb Survivors: Results from 25...

Continuous intra-jugular venous blood-gas monitoring with the Paratrend 7 during hypothermic cardiopulmonary bypass (2001)

Endoh, H., Honda, T., Oohashi, S., Nagata, Y., Shibue, C., Shimoji, K.

We measured the accuracy of the continuous intra‐vascular blood‐gas monitoring system (Paratrend 7, PT7) placed in the jugular venous bulb in 18 adult patients having cardiac or aortic...

Dry sliding wear behavior of $Al_{2}O_{3}$ fiber reinforced aluminum composites (2000)

Iwai, Y, Honda, T, Miyajima, T, Iwasaki, Y, Surappa, MK, Xu, JF

Dry sliding wear behavior of die-cast ADC12 aluminum alloy composites reinforced with short alumina fibers were investigated by using a pin-on-disk wear tester. The Al2O3 fibers were 4 mu m in...

High Performance 4600 Steels by Injection Molding. (1998)

Miura, H., Honda, T., Hens, K. F., German, R. M.

This study with powder injection molding considers the combined influences of type of powders and subsequent debinding and sintering conditions on the control of carbon content and structure for 4600...

Effect of Pb on High-Tc Phase Formation and Properties of Bi(2(1-x))Pb(2x)Sr2Ca2Cu3O(y). (1998)

Honda, T., Meskoob, M., Safari, A., Wachtman, J. B.

We prepared Bi(2(1-x))Pb(2x)Sr2Cu3O(y) (x=0, 0.1, 0.2, 0.3, and 0.5) by conventional solid state reactions with various firing temperatures and carried out X-ray diffraction analysis and resistivity...

Stability of the maximum density droplet in quantum dots at high magnetic fields (1998)

Oosterkamp, T. H., Janssen, J. W., Kouwenhoven, L. P., Austing, D. G., Honda, T., Tarucha, S.

We have measured electron transport through a vertical quantum dot containing a tunable number of electrons between 0 and 40. Over some region in magnetic field the electrons are spin polarized and...

Non-isochromaticity of an omega filter in a 200 kV transmission electron microscope (1998)

Tsuno, K., Kaneyama, T., Honda, T., Ishida, Y.

Non-isochromaticity of a newly developed energy filtering transmission electron microscope (EFTEM), JEM2010FEF, has been evaluated. The nonisochromaticity is the difference in energy from position to...

Excitation Spectra of Circular Few-Electron Quantum Dots (1997)

Kouwenhoven, L. P., Oosterkamp, T. H., Danoesastro, M. W. S., Eto, M., Austing, D. G., Honda, T., ...

We study ground states and excited states in semiconductor quantum dots containing 1 to 12 electrons. For the first time, it is possible to identify the quantum numbers of the states in the...

Results of Endoscopic Treatment for Early Gastric Cancer by Nd-YAG Laser (1997)

M. Tani, K. Takeshita, T. Honda, N. Saito, M. Endo

We have introduced two endoscopic treatments for early gastric cancer: endoscopic mucosal resection using a cap-fitted panendoscope (EMRC), and endoscopic laser therapy using a Nd-YAG laser....

Design and testing of Omega mode imaging energy filters at 200 kV (1997)

Tsuno, K., Kaneyama, T., Honda, T., Tsuda, K., Terauchi, M., Tanaka, M.

We designed and constructed an omega filter with following optical properties. The dispersions at 100 and 200 kV are 2.1 and 1.1 μm eV-1, respectively. Non-isochromatisity (difference of the...

SURFACE DIFFUSION OF ATOMS ON GERMANIUM SURFACES MEASURED BY COUNTING-FLUCTUATIONS OF FIELD-EMITTED ELECTRONS (1988)

Honda, T., Okano, T., Tuzi, Y.

Surface diffusion of Ga atoms on a Ge(100) plane was studied by using digital autocorrelation analysis of field-emitted electrons. The present method was based on pulse-counting of field-emitted...

SURFACE DIFFUSION OF ATOMS ON GERMANIUM SURFACES MEASURED BY COUNTING-FLUCTUATIONS OF FIELD-EMITTED ELECTRONS (1988)

Honda, T., Okano, T., Tuzi, Y.

Surface diffusion of Ga atoms on a Ge(100) plane was studied by using digital autocorrelation analysis of field-emitted electrons. The present method was based on pulse-counting of field-emitted...

SURFACE DIFFUSION OF ATOMS ON GERMANIUM SURFACES MEASURED BY COUNTING-FLUCTUATIONS OF FIELD-EMITTED ELECTRONS (1988)

Honda, T., Okano, T., Tuzi, Y.

Surface diffusion of Ga atoms on a Ge(100) plane was studied by using digital autocorrelation analysis of field-emitted electrons. The present method was based on pulse-counting of field-emitted...

MECANISMES D'INTERACTION DANS LA REACTION 11B (p, α) 8Be (0+) (1970)

Cavaignac, J., Longequeue, N., Honda, T.

La réaction 11B (p, α) 8Be (0+) est étudiée à Ep = 12, 20, 24 e t 30 MeV. Une analyse par ondes planes réalisée en tenant compte des processus de "Pick- up" et "heavy particle pick up"...

MECANISMES D'INTERACTION DANS LA REACTION 11B (p, α) 8Be (0+) (1970)

Cavaignac, J., Longequeue, N., Honda, T.

La réaction 11B (p, α) 8Be (0+) est étudiée à Ep = 12, 20, 24 e t 30 MeV. Une analyse par ondes planes réalisée en tenant compte des processus de "Pick- up" et "heavy particle pick up"...

MECANISMES D'INTERACTION DANS LA REACTION 11B (p, α) 8Be (0+) (1970)

Cavaignac, J., Longequeue, N., Honda, T.

La réaction 11B (p, α) 8Be (0+) est étudiée à Ep = 12, 20, 24 e t 30 MeV. Une analyse par ondes planes réalisée en tenant compte des processus de "Pick- up" et "heavy particle pick up"...

Molecular cloning, sequencing, tissue distribution, and functional expression of a Na+/H+ exchanger (NHE-2).

Collins, J F, Honda, T, Knobel, S, Bulus, N M, Conary, J, DuBois, R, ...

The present studies demonstrate cloning, sequencing, tissue distribution, and functional expression of a Na+/H+ exchanger which was isolated from a rat intestinal cDNA library. The cloned cDNA...

A novel method to chemically immobilize antibody on nylon and its application to the rapid and differential detection of two Vibrio parahaemolyticus toxins in a modified enzyme-linked immunosorbent assay.

Honda, T, Miwatani, T, Yabushita, Y, Koike, N, Okada, K

A new method of chemically immobilizing antibody on nylon was developed. The method consists of serial treatments with HCl, polyethylene imine, and maleic anhydride methylvinyl ether copolymer, which...

Sequencing of the gene encoding the major pilin of pilus colonization factor antigen III (CFA/III) of human enterotoxigenic Escherichia coli and evidence that CFA/III is related to type IV pili.

Taniguchi, T, Fujino, Y, Yamamoto, K, Miwatani, T, Honda, T

The plasmid-encoded structural gene cofA necessary for the production of the major pilin subunit of pilus colonization factor antigen III (CFA/III) of human enterotoxigenic Escherichia coli was...

Purification and characterization of a cell-associated hemagglutinin of Vibrio parahaemolyticus.

Nagayama, K, Oguchi, T, Arita, M, Honda, T

We found a positive correlation between cell-associated mannose-sensitive hemagglutination and adherence of Vibrio parahaemolyticus to rabbit enterocytes by investigating 35 strains of V....

In vitro proteolytic processing and activation of the recombinant precursor of El Tor cytolysin/hemolysin (pro-HlyA) of Vibrio cholerae by soluble hemagglutinin/protease of V. cholerae, trypsin, and other proteases.

Nagamune, K, Yamamoto, K, Naka, A, Matsuyama, J, Miwatani, T, Honda, T

Vibrio cholerae produces a cytolytic toxin named El Tor cytolysin/hemolysin which is encoded by the hlyA gene. This cytolysin is produced as a 79-kDa precursor form (pro-HlyA) into the culture...

Cholera enterotoxin production in Vibrio cholerae O1 strains isolated from the environment and from humans in Japan.

Minami, A, Hashimoto, S, Abe, H, Arita, M, Taniguchi, T, Honda, T, ...

Vibrio cholerae O1 strains isolated from various sources in Japan over the years 1977 through 1987 were examined to confirm the presence or absence of the cholera enterotoxin (CT) gene and production...

Demonstration and characterization of simultaneous production of a thermostable direct hemolysin (TDH/I) and a TDH-related hemolysin (TRHx) by a clinically isolated Vibrio parahaemolyticus strain, TH3766.

Xu, M, Iida, T, Yamamoto, K, Takarada, Y, Miwatani, T, Honda, T

Simultaneous production of a thermostable direct hemolysin (TDH)-like toxin (TDHx) and a TDH-related hemolysin (TRH)-like toxin (TRHx) by a clinical isolate (strain TH3766) of Kanagawa...

Construction and characterization of an isogenic mutant of Vibrio parahaemolyticus having a deletion in the thermostable direct hemolysin-related hemolysin gene (trh)

Xu, M, Yamamoto, K, Honda, T

A mutant, Vp-MX02, having a deletion in the gene (trh) for the thermostable direct hemolysin-related hemolysin (TRH) was constructed by double-crossover gene conversion from a TRH-producing Vibrio...

Comparative amino acid sequence analysis of hemolysins produced by Vibrio hollisae and Vibrio parahaemolyticus.

Yoh, M, Honda, T, Miwatani, T, Tsunasawa, S, Sakiyama, F

Vibrio hollisae produces a hemolysin (Vh-rTDH) that is related to the thermostable direct hemolysin of Vibrio parahaemolyticus (Vp-TDH). Although both hemolysins are essentially similar biologically...

Effect of substitution of glycine for arginine at position 146 of the A1 subunit on biological activity of Escherichia coli heat-labile enterotoxin.

Okamoto, K, Okamoto, K, Miyama, A, Tsuji, T, Honda, T, Miwatani, T

The ADP-ribosyltransferase activity of polypeptide A1 of cholera toxin and that of Escherichia coli heat-labile enterotoxin (LT) are primarily responsible for the toxic activities of these toxins....

Restriction fragment length polymorphism of the tdh and trh genes in clinical Vibrio parahaemolyticus strains.

Suthienkul, O, Iida, T, Park, K S, Ishibashi, M, Supavej, S, Yamamoto, K, ...

The restriction fragment length polymorphism of the genes encoding thermostable direct hemolysin (tdh) and thermostable direct hemolysin-related hemolysin (trh) was analyzed for 137 strains of Vibrio...

Use of an alkaline phosphatase-conjugated oligonucleotide probe for the gene encoding the bundle-forming pilus of enteropathogenic Escherichia coli.

Nagayama, K, Bi, Z, Oguchi, T, Takarada, Y, Shibata, S, Honda, T

An alkaline phosphatase-conjugated 29-base oligonucleotide probe was developed to detect the gene encoding the bundle-forming pilus of enteropathogenic Escherichia coli. The sensitivity and...

Demonstration of a plasmid-borne gene encoding a thermostable direct hemolysin in Vibrio cholerae non-O1 strains.

Honda, T, Nishibuchi, M, Miwatani, T, Kaper, J B

Of 15 Vibrio cholerae non-O1 strains, 2 produced a hemolysin termed NAG-rTDH, which is very similar to the thermostable direct hemolysin of V. parahaemolyticus. These two strains contained DNA...

Purification and characterization of a new heat-stable enterotoxin produced by Vibrio cholerae non-O1 serogroup Hakata.

Arita, M, Honda, T, Miwatani, T, Ohmori, K, Takao, T, Shimonishi, Y

The possible production of a heat-stable enterotoxin (Vc-H-ST) by Vibrio cholerae non-O1 serogroup Hakata was investigated, and the purified Vc-H-ST was characterized. It has a unique amino acid...

Purification and characterization of Vibrio metschnikovii cytolysin.

Miyake, M, Honda, T, Miwatani, T

An extracellular cytolysin produced by Vibrio metschnikovii was purified by acid precipitation, phenyl-Sepharose CL-4B chromatography, and rechromatography on a phenyl-Sepharose CL-4B column and...

Purification and characterization of a hemolysin produced by a clinical isolate of Kanagawa phenomenon-negative Vibrio parahaemolyticus and related to the thermostable direct hemolysin.

Honda, T, Ni, Y X, Miwatani, T

A clinical isolate (strain 4037) of Kanagawa phenomenon-negative Vibrio parahaemolyticus was studied. Although the strain was judged to be Kanagawa phenomenon-negative by various conventional tests,...

Isolation of Shiga toxin-resistant Vero cells and their use for easy identification of the toxin.

Kongmuang, U, Honda, T, Miwatani, T

Shiga toxin-resistant Vero cells were isolated by treatment of the cells with nitrosoguanidine. These mutant cells were not affected by Shiga toxin at more than 1 microgram/ml, although the parent...

Production and characterization of monoclonal antibodies to a pilus colonization factor (colonization factor antigen III) of human enterotoxigenic Escherichia coli.

Honda, T, Wetprasit, N, Arita, M, Miwatani, T

Three monoclonal antibodies (MAbs) to a pilus colonization factor (colonization factor antigen III [CFA/III]) of human enterotoxigenic Escherichia coli (ETEC) were developed and characterized. All of...

Comparative study of Vibrio cholerae non-O1 protease and soluble hemagglutinin with those of Vibrio cholerae O1.

Honda, T, Booth, B A, Boesman-Finkelstein, M, Finkelstein, R A

Protease and soluble hemagglutinating activities produced by a non-O1 Vibrio cholerae strain isolated from a patient with diarrhea were compared with similar activities produced by V. cholerae O1....

Comparison of effects of nicked and unnicked Escherichia coli heat-labile enterotoxin on Chinese hamster ovary cells.

Tsuji, T, Honda, T, Miwatani, T

The effect on nicking (proteolytic cleavage) of heat-labile enterotoxin (LT) on its activities on Chinese hamster ovary (CHO) cells was examined. LT nicked with trypsin induced morphological change...

Demonstration of cholera toxin-related factor in cultures of Aeromonas species by enzyme-linked immunosorbent assay.

Honda, T, Sato, M, Nishimura, T, Higashitsutsumi, M, Fukai, K, Miwatani, T

The production of toxins by Aeromonas species was examined by the suckling mouse test, the hemolysin test, and the enzyme-linked immunosorbent assay with anticholera enterotoxin. A factor that was...

Purification and characterization of Vibrio cholerae non-O1 heat-stable enterotoxin.

Arita, M, Takeda, T, Honda, T, Miwatani, T

A toxin which causes rapid fluid accumulation in a suckling mouse assay and which was produced by Vibrio cholerae non-O1 was investigated. The toxin was purified from the culture supernatant of V....

Purification and partial characterization of a non-O1 Vibrio cholerae hemolysin that cross-reacts with thermostable direct hemolysin of Vibrio parahaemolyticus.

Yoh, M, Honda, T, Miwatani, T

A newly identified hemolysin (NAG-rTDH), which is related to the thermostable direct hemolysin (Vp-TDH) of Vibrio parahaemolyticus produced by non-O1 Vibrio cholerae, was studied. NAG-rTDH was...

Development of an enzyme-labeled oligonucleotide probe for the cholera toxin gene.

Yoh, M, Miyagi, K, Matsumoto, Y, Hayashi, K, Takarada, Y, Yamamoto, K, ...

An alkaline phosphatase-conjugated 30-mer oligonucleotide probe was developed to detect the cholera toxin gene (ctx) in Vibrio cholerae O1. For rapid identification, V. cholerae O1 was grown on...

Non-O1 Vibrio cholerae hemolysin: purification, partial characterization, and immunological relatedness to El Tor hemolysin.

Yamamoto, K, Al-Omani, M, Honda, T, Takeda, Y, Miwatani, T

Hemolysin of a non-O1 Vibrio cholerae strain was purified and characterized. The purified hemolysin gave a single protein band on conventional and sodium dodecyl sulfate-gel electrophoresis. Its...

Development of enzyme-labeled oligonucleotide probe for detection of mecA gene in methicillin-resistant Staphylococcus aureus.

Shimaoka, M, Yoh, M, Segawa, A, Takarada, Y, Yamamoto, K, Honda, T

A DNA hybridization method with an enzyme-labeled oligonucleotide probe (mecA-ELONP) was developed to detect the methicillin-resistant gene (mecA) in methicillin-resistant Staphylococcus aureus. For...

Characterization of new hydrophobic pili of human enterotoxigenic Escherichia coli: a possible new colonization factor.

Honda, T, Arita, M, Miwatani, T

Enterotoxigenic Escherichia coli strains were divided into five groups on the basis of their bacterial surface hydrophobicity (Honda et al., FEMS Microbiol. Lett. 17:273-276, 1983). Strains in group...

Evaluation in humans of attenuated Vibrio cholerae El Tor Ogawa strain Texas Star-SR as a live oral vaccine.

Levine, M M, Black, R E, Clements, M L, Lanata, C, Sears, S, Honda, T, ...

Texas Star-SR, an A- B+ mutant derived by nitrosoguanidine treatment from Vibrio cholerae El Tor Ogawa strain 3083, was fed to 68 volunteers as an oral vaccine in doses of 10(5) to 5 X 10(10)...

Effects of lincomycin and tetracycline on production and properties of enterotoxins of enterotoxigenic Escherichia coli.

Yoh, M, Yamamoto, K, Honda, T, Takeda, Y, Miwatani, T

Enterotoxigenic Escherichia coli grown in the presence of lincomycin and tetracycline produced an increased amount of heat-labile enterotoxin (LT). These antibiotics increased the production of not...

Analysis of antigenic determinants in cholera enterotoxin and heat-labile enterotoxins from human and porcine enterotoxigenic Escherichia coli.

Takeda, Y, Honda, T, Sima, H, Tsuji, T, Miwatani, T

Antigenic determinants of cholera enterotoxin (CT) and heat-labile enterotoxin from a human strain (LTh) and a porcine strain (LTp) were analyzed by Ouchterlony double-gel diffusion test against...

Enzyme-linked immunosorbent assay to detect Shiga toxin of Shigella dysenteriae and related toxins.

Kongmuang, U, Honda, T, Miwatani, T

A sandwich enzyme-linked immunosorbent assay (ELISA) was developed for detection of Shiga toxin. Four species of Shigella, Escherichia coli, and Vibro parahaemolyticus were tested for production of...

Evaluation of a nonisotopically labeled oligonucleotide probe to detect the heat-stable enterotoxin gene of Escherichia coli by the DNA colony hybridization test.

Nishibuchi, M, Arita, M, Honda, T, Miwatani, T

A commercially available, alkaline-phosphatase-conjugated oligonucleotide probe for detecting the heat-stable enterotoxin gene of Escherichia coli was compared with cloned gene probes by examining E....

Detection with synthetic oligonucleotide probes of nucleotide sequence variations in the genes encoding enterotoxins of Escherichia coli.

Nishibuchi, M, Murakami, A, Arita, M, Jikuya, H, Takano, J, Honda, T, ...

We examined variations in the genes encoding heat-stable enterotoxin (ST) and heat-labile enterotoxin (LT) in 88 strains of Escherichia coli isolated from individuals with traveler's diarrhea to find...

Enzyme-linked immunosorbent assays for detection of thermostable direct hemolysin of Vibrio parahaemolyticus.

Honda, T, Yoh, M, Kongmuang, U, Miwatani, T

Several systems for enzyme-linked immunosorbent assay (ELISA) of thermostable direct hemolysin (TDH) of Vibrio parahaemolyticus were tested, and single-antibody sandwich ELISA systems gave...

Comparison of preservation methods for enterotoxigenic Escherichia coli producing heat-labile enterotoxin.

Yoh, M, Narita, I, Honda, T, Miwatani, T, Nishibuchi, M

Ten strains of enterotoxigenic Escherichia coli producing heat-labile enterotoxin (LT) were preserved under 12 different conditions. After 1 month, 9 months, and 3 years of preservation, the cultures...

Differential detection of cholera enterotoxin and Escherichia coli heat-labile enterotoxin by enzyme-linked immunosorbent assays with antibodies specific to the two toxins.

Honda, T, Sato, M, Miwatani, T

Enzyme-linked immunosorbent assays (ELISAs) with antibodies specific to either cholera enterotoxin (CT) of Vibrio cholerae or heat-labile enterotoxin (LT) of enterotoxigenic Escherichia coli were...

Further evaluation of the Biken test (modified Elek test) for detection of enterotoxigenic Escherichia coli producing heat-labile enterotoxin and application of the test to sampling of heat-stable enterotoxin.

Honda, T, Arita, M, Takeda, Y, Miwatani, T

A total of 2,229 strains of Escherichia coli isolated from subjects with traveller's diarrhea were tested for heat-labile enterotoxin production by the Biken test (Honda et al., J. Clin. Microbiol....

Immunological detection of the Kanagawa phenomenon of vibrio parahaemolyticus on modified selective media.

Honda, T, Sornchai, C, Takeda, Y, Miwatani, T

Selective media for Vibrio parahaemolyticus, BTB-Teepol agar and modified arabinose-ammonium sulfate-cholate agar, were modified for use in immunological detection of the thermostable direct...

Detection by a staphylococcal coagglutination test of heat-labile enterotoxin-producing enterotoxigenic Escherichia coli.

Honda, T, Samakoses, R, Sornchai, C, Takeda, Y, Miwatani, T

For detection of heat-labile enterotoxin-producing enterotoxigenic Escherichia coli, the staphylococcal coagglutination test reported by Brill et al. (J. Clin. Microbiol. 9:49-55, 1979) was modified...

Immunological methods for detection of Kanagawa phenomenon of Vibrio parahaemolyticus.

Honda, T, Chearskul, S, Takeda, Y, Miwatani, T

A modified Elek test and an immuno-halo test were developed for detection of the thermostable direct hemolysin produced by Kanagawa phenomenon-positive strains of Vibrio parahaemolyticus. The...

Modified Elek test for detection of heat-labile enterotoxin of enterotoxigenic Escherichia coli.

Honda, T, Taga, S, Takeda, Y, Miwatani, T

The Elek test was modified for detection of the heat-labile enterotoxin of enterotoxigenic Escherichia coli. A total of 164 strains of E. coli were tested by the modified Elek test, and the results...

A mutant toxin of Vibrio parahaemolyticus thermostable direct hemolysin which has lost hemolytic activity but retains ability to bind to erythrocytes.

Tang, G Q, Iida, T, Yamamoto, K, Honda, T

A mutant toxin, R7, of thermostable direct hemolysin (TDH) with a single amino acid substitution at glycine 62 was analyzed. The hemolytic activity of R7 decreased to less than 1/1,000 of that of...

Effects of divalent cations and saccharides on Vibrio metschnikovii cytolysin-induced hemolysis of rabbit erythrocytes.

Miyake, M, Honda, T, Miwatani, T

Divalent cations and polysaccharides such as inulin and dextran reversibly inhibited hemolysis of rabbit erythrocytes caused by Vibrio metschnikovii cytolysin. On the basis of the 50% inhibitory...

Cloning and nucleotide sequence of the gene (trh) encoding the hemolysin related to the thermostable direct hemolysin of Vibrio parahaemolyticus.

Nishibuchi, M, Taniguchi, T, Misawa, T, Khaeomanee-Iam, V, Honda, T, Miwatani, T

Vibrio parahaemolyticus isolates derived from an outbreak of gastroenteritis in the Republic of Maldives did not have the genetic potential to produce the thermostable direct hemolysin, but one such...

Purification and characterization of a protease produced by Vibrio cholerae non-O1 and comparison with a protease of V. cholerae O1.

Honda, T, Lertpocasombat, K, Hata, A, Miwatani, T, Finkelstein, R A

A protease produced by a clinical isolate of Vibrio cholerae non-O1 was purified to apparent homogeneity by ammonium sulfate fractionation and successive column chromatography on DEAE-Sephadex A25,...

Two-step processing for activation of the cytolysin/hemolysin of Vibrio cholerae O1 biotype El Tor: nucleotide sequence of the structural gene (hlyA) and characterization of the processed products.

Yamamoto, K, Ichinose, Y, Shinagawa, H, Makino, K, Nakata, A, Iwanaga, M, ...

Vibrio cholerae O1 biotype El Tor produces and secretes a 65-kDa cytolysin/hemolysin into the culture medium. We cloned the structural gene (hlyA) for the cytolysin from the total DNA of a V....

Molecular heterogeneity of heat-labile enterotoxins from human and porcine enterotoxigenic Escherichia coli.

Tsuji, T, Taga, S, Honda, T, Takeda, Y, Miwatani, T

The heat-labile enterotoxins produced by human enterotoxigenic Escherichia coli (LTh) and porcine enterotoxigenic E. coli (LTp) were purified to homogeneity, and their molecular properties were...

Isolation of special antibodies which react only with homologous enterotoxins from Vibrio cholerae and Enterotoxigenic Escherichia coli.

Honda, T, Takeda, Y, Miwatani, T

Cholera enterotoxin (CT) and heat-labile enterotoxin (LT) produced by enterotoxigenic Escherichia coli share a common antigenic determinant. In addition, CT and LT each have a unique antigenic...

Immunological nonidentity of heat-labile enterotoxins from human and porcine enterotoxigenic Escherichia coli.

Honda, T, Tsuji, T, Takeda, Y, Miwatani, T

The Ouchterlony double-gel diffusion test demonstrated that heat-labile enterotoxin (LT) from human strains of enterotoxigenic Escherichia coli was antigenically related but not identical to that...

In vitro formation of hybrid toxins between subunits of Escherichia coli heat-labile enterotoxin and those of cholera enterotoxin.

Takeda, Y, Honda, T, Taga, S, Miwatani, T

Heat-labile enterotoxin (LT) was purified from cells of enterotoxigenic Escherichia coli isolated from a patient with traveller's diarrhea. Purified LT was separated into A and B subunits by...

Failure of chlorpromazine to inhibit fluid accumulation caused by Escherichia coli heat-stable enterotoxin in suckling mice.

Takeda, T, Honda, T, Takeda, Y, Miwatani, T

We studied the effect of chlorpromazine on fluid accumulation caused by purified heat-stable enterotoxin (ST) from enterotoxigenic Escherichia coli in suckling mice. We found that chlorpromazine...

Selection and characteristics of a Vibrio cholerae mutant lacking the A (ADP-ribosylating) portion of the cholera enterotoxin.

Honda, T, Finkelstein, R A

After mutagenesis with nitrosoguanidine and selection by immuno-halo techniques, an avirulent mutant, designated Texas Star-SR, which produces no detectable A (active; ADP-ribosylating) region of the...

Purification and characterization of a hemolysin produced by Vibrio cholerae biotype El Tor: another toxic substance produced by cholera vibrios.

Honda, T, Finkelstein, R A

A thermolabile direct hemolysin from an El Tor cholera vibrio strain has been isolated and partially characterized as a simple protein of ca. 20,000 molecular weight. In addition to its hemolytic...

Inhibition of hemolytic activity of the thermostable direct hemolysin of Vibrio parahaemolyticus by ganglioside.

Takeda, R, Honda, T, Sakural, J, Otomo, N

The hemolytic activity of the thermostable direct hemolysin produced by Vibrio parahaemolyticus was inhibited by a ganglioside mixture. The ganglioside component which inhibited the hemolysin was not...

Isolation of a factor causing morphological changes of chinese hamster ovary cells from the culture filtrate of Vibrio parahaemolyticus.

Honda, T, Shimizu, M, Takeda, Y, Miwatani, T

A factor changing Chinese hamster ovary cells from an oval to a spindle shape was isolated from the culture filtrate of Vibrio parahaemolyticus. It was partially purified by successive column...

Identification of lethal toxin with the thermostable direct hemolysin produced by Vibrio parahaemolyticus, and some physicochemical properties of the purified toxin.

Honda, T, Taga, S, Takeda, T, Hasibuan, M A, Takeda, Y, Miwatani, T

Lethal toxin was purified extensively from the culture filtrate of a Kanagawa phenomenon-positive strain of Vibrio parahaemolyticus. The purified toxin was a protein, and its homogeneity was...

Demonstration of the cardiotoxicity of the thermostable direct hemolysin (lethal toxin) produced by Vibrio parahaemolyticus.

Honda, T, Goshima, K, Takeda, Y, Sugino, Y, Miwatani, T

Intravenous injection of the thermostable direct hemolysin (lethal toxin) produced by Vibrio parahaemolyticus caused rapid death of rats. Studies by electroencephalography and electrocardiography...

Cytotoxic effect of the thermostable direct hemolysin produced by Vibrio parahaemolyticus on FL cells.

Sakurai, J, Honda, T, Jinguji, Y, Arita, M, Miwatani, T

The thermostable direct hemolysin produced by Vibrio parahaemolyticus showed cytotoxic activity on FL cells derived from human amniotic membrane. Scanning electron micrographs of the whole cells...

Inactivation of the biological activities of the thermostable direct hemolysin of Vibrio parahaemolyticus by ganglioside Gt1.

Takeda, Y, Takeda, T, Honda, T, Miwatani, T

Biological activities of the thermostable direct hemolysin produced by Vibrio parahaemolyticus, such as its hemolytic activity and lethal activity, were inhibited by neuraminidase-sensitive...

Molecular cloning, sequencing, tissue distribution, and functional expression of a Na+/H+ exchanger (NHE-2).

Collins, J F, Honda, T, Knobel, S, Bulus, N M, Conary, J, DuBois, R, ...

The present studies demonstrate cloning, sequencing, tissue distribution, and functional expression of a Na+/H+ exchanger which was isolated from a rat intestinal cDNA library. The cloned cDNA...

A novel method to chemically immobilize antibody on nylon and its application to the rapid and differential detection of two Vibrio parahaemolyticus toxins in a modified enzyme-linked immunosorbent assay.

Honda, T, Miwatani, T, Yabushita, Y, Koike, N, Okada, K

A new method of chemically immobilizing antibody on nylon was developed. The method consists of serial treatments with HCl, polyethylene imine, and maleic anhydride methylvinyl ether copolymer, which...

Sequencing of the gene encoding the major pilin of pilus colonization factor antigen III (CFA/III) of human enterotoxigenic Escherichia coli and evidence that CFA/III is related to type IV pili.

Taniguchi, T, Fujino, Y, Yamamoto, K, Miwatani, T, Honda, T

The plasmid-encoded structural gene cofA necessary for the production of the major pilin subunit of pilus colonization factor antigen III (CFA/III) of human enterotoxigenic Escherichia coli was...

Purification and characterization of a cell-associated hemagglutinin of Vibrio parahaemolyticus.

Nagayama, K, Oguchi, T, Arita, M, Honda, T

We found a positive correlation between cell-associated mannose-sensitive hemagglutination and adherence of Vibrio parahaemolyticus to rabbit enterocytes by investigating 35 strains of V....

In vitro proteolytic processing and activation of the recombinant precursor of El Tor cytolysin/hemolysin (pro-HlyA) of Vibrio cholerae by soluble hemagglutinin/protease of V. cholerae, trypsin, and other proteases.

Nagamune, K, Yamamoto, K, Naka, A, Matsuyama, J, Miwatani, T, Honda, T

Vibrio cholerae produces a cytolytic toxin named El Tor cytolysin/hemolysin which is encoded by the hlyA gene. This cytolysin is produced as a 79-kDa precursor form (pro-HlyA) into the culture...

Cholera enterotoxin production in Vibrio cholerae O1 strains isolated from the environment and from humans in Japan.

Minami, A, Hashimoto, S, Abe, H, Arita, M, Taniguchi, T, Honda, T, ...

Vibrio cholerae O1 strains isolated from various sources in Japan over the years 1977 through 1987 were examined to confirm the presence or absence of the cholera enterotoxin (CT) gene and production...

Demonstration and characterization of simultaneous production of a thermostable direct hemolysin (TDH/I) and a TDH-related hemolysin (TRHx) by a clinically isolated Vibrio parahaemolyticus strain, TH3766.

Xu, M, Iida, T, Yamamoto, K, Takarada, Y, Miwatani, T, Honda, T

Simultaneous production of a thermostable direct hemolysin (TDH)-like toxin (TDHx) and a TDH-related hemolysin (TRH)-like toxin (TRHx) by a clinical isolate (strain TH3766) of Kanagawa...

Construction and characterization of an isogenic mutant of Vibrio parahaemolyticus having a deletion in the thermostable direct hemolysin-related hemolysin gene (trh)

Xu, M, Yamamoto, K, Honda, T

A mutant, Vp-MX02, having a deletion in the gene (trh) for the thermostable direct hemolysin-related hemolysin (TRH) was constructed by double-crossover gene conversion from a TRH-producing Vibrio...

Comparative amino acid sequence analysis of hemolysins produced by Vibrio hollisae and Vibrio parahaemolyticus.

Yoh, M, Honda, T, Miwatani, T, Tsunasawa, S, Sakiyama, F

Vibrio hollisae produces a hemolysin (Vh-rTDH) that is related to the thermostable direct hemolysin of Vibrio parahaemolyticus (Vp-TDH). Although both hemolysins are essentially similar biologically...

Effect of substitution of glycine for arginine at position 146 of the A1 subunit on biological activity of Escherichia coli heat-labile enterotoxin.

Okamoto, K, Okamoto, K, Miyama, A, Tsuji, T, Honda, T, Miwatani, T

The ADP-ribosyltransferase activity of polypeptide A1 of cholera toxin and that of Escherichia coli heat-labile enterotoxin (LT) are primarily responsible for the toxic activities of these toxins....

Restriction fragment length polymorphism of the tdh and trh genes in clinical Vibrio parahaemolyticus strains.

Suthienkul, O, Iida, T, Park, K S, Ishibashi, M, Supavej, S, Yamamoto, K, ...

The restriction fragment length polymorphism of the genes encoding thermostable direct hemolysin (tdh) and thermostable direct hemolysin-related hemolysin (trh) was analyzed for 137 strains of Vibrio...

Use of an alkaline phosphatase-conjugated oligonucleotide probe for the gene encoding the bundle-forming pilus of enteropathogenic Escherichia coli.

Nagayama, K, Bi, Z, Oguchi, T, Takarada, Y, Shibata, S, Honda, T

An alkaline phosphatase-conjugated 29-base oligonucleotide probe was developed to detect the gene encoding the bundle-forming pilus of enteropathogenic Escherichia coli. The sensitivity and...

Demonstration of a plasmid-borne gene encoding a thermostable direct hemolysin in Vibrio cholerae non-O1 strains.

Honda, T, Nishibuchi, M, Miwatani, T, Kaper, J B

Of 15 Vibrio cholerae non-O1 strains, 2 produced a hemolysin termed NAG-rTDH, which is very similar to the thermostable direct hemolysin of V. parahaemolyticus. These two strains contained DNA...

Purification and characterization of a new heat-stable enterotoxin produced by Vibrio cholerae non-O1 serogroup Hakata.

Arita, M, Honda, T, Miwatani, T, Ohmori, K, Takao, T, Shimonishi, Y

The possible production of a heat-stable enterotoxin (Vc-H-ST) by Vibrio cholerae non-O1 serogroup Hakata was investigated, and the purified Vc-H-ST was characterized. It has a unique amino acid...

Purification and characterization of Vibrio metschnikovii cytolysin.

Miyake, M, Honda, T, Miwatani, T

An extracellular cytolysin produced by Vibrio metschnikovii was purified by acid precipitation, phenyl-Sepharose CL-4B chromatography, and rechromatography on a phenyl-Sepharose CL-4B column and...

Purification and characterization of a hemolysin produced by a clinical isolate of Kanagawa phenomenon-negative Vibrio parahaemolyticus and related to the thermostable direct hemolysin.

Honda, T, Ni, Y X, Miwatani, T

A clinical isolate (strain 4037) of Kanagawa phenomenon-negative Vibrio parahaemolyticus was studied. Although the strain was judged to be Kanagawa phenomenon-negative by various conventional tests,...

Isolation of Shiga toxin-resistant Vero cells and their use for easy identification of the toxin.

Kongmuang, U, Honda, T, Miwatani, T

Shiga toxin-resistant Vero cells were isolated by treatment of the cells with nitrosoguanidine. These mutant cells were not affected by Shiga toxin at more than 1 microgram/ml, although the parent...

Production and characterization of monoclonal antibodies to a pilus colonization factor (colonization factor antigen III) of human enterotoxigenic Escherichia coli.

Honda, T, Wetprasit, N, Arita, M, Miwatani, T

Three monoclonal antibodies (MAbs) to a pilus colonization factor (colonization factor antigen III [CFA/III]) of human enterotoxigenic Escherichia coli (ETEC) were developed and characterized. All of...

Comparative study of Vibrio cholerae non-O1 protease and soluble hemagglutinin with those of Vibrio cholerae O1.

Honda, T, Booth, B A, Boesman-Finkelstein, M, Finkelstein, R A

Protease and soluble hemagglutinating activities produced by a non-O1 Vibrio cholerae strain isolated from a patient with diarrhea were compared with similar activities produced by V. cholerae O1....

Comparison of effects of nicked and unnicked Escherichia coli heat-labile enterotoxin on Chinese hamster ovary cells.

Tsuji, T, Honda, T, Miwatani, T

The effect on nicking (proteolytic cleavage) of heat-labile enterotoxin (LT) on its activities on Chinese hamster ovary (CHO) cells was examined. LT nicked with trypsin induced morphological change...

Demonstration of cholera toxin-related factor in cultures of Aeromonas species by enzyme-linked immunosorbent assay.

Honda, T, Sato, M, Nishimura, T, Higashitsutsumi, M, Fukai, K, Miwatani, T

The production of toxins by Aeromonas species was examined by the suckling mouse test, the hemolysin test, and the enzyme-linked immunosorbent assay with anticholera enterotoxin. A factor that was...

Purification and characterization of Vibrio cholerae non-O1 heat-stable enterotoxin.

Arita, M, Takeda, T, Honda, T, Miwatani, T

A toxin which causes rapid fluid accumulation in a suckling mouse assay and which was produced by Vibrio cholerae non-O1 was investigated. The toxin was purified from the culture supernatant of V....

Purification and partial characterization of a non-O1 Vibrio cholerae hemolysin that cross-reacts with thermostable direct hemolysin of Vibrio parahaemolyticus.

Yoh, M, Honda, T, Miwatani, T

A newly identified hemolysin (NAG-rTDH), which is related to the thermostable direct hemolysin (Vp-TDH) of Vibrio parahaemolyticus produced by non-O1 Vibrio cholerae, was studied. NAG-rTDH was...

Development of an enzyme-labeled oligonucleotide probe for the cholera toxin gene.

Yoh, M, Miyagi, K, Matsumoto, Y, Hayashi, K, Takarada, Y, Yamamoto, K, ...

An alkaline phosphatase-conjugated 30-mer oligonucleotide probe was developed to detect the cholera toxin gene (ctx) in Vibrio cholerae O1. For rapid identification, V. cholerae O1 was grown on...

Non-O1 Vibrio cholerae hemolysin: purification, partial characterization, and immunological relatedness to El Tor hemolysin.

Yamamoto, K, Al-Omani, M, Honda, T, Takeda, Y, Miwatani, T

Hemolysin of a non-O1 Vibrio cholerae strain was purified and characterized. The purified hemolysin gave a single protein band on conventional and sodium dodecyl sulfate-gel electrophoresis. Its...

Development of enzyme-labeled oligonucleotide probe for detection of mecA gene in methicillin-resistant Staphylococcus aureus.

Shimaoka, M, Yoh, M, Segawa, A, Takarada, Y, Yamamoto, K, Honda, T

A DNA hybridization method with an enzyme-labeled oligonucleotide probe (mecA-ELONP) was developed to detect the methicillin-resistant gene (mecA) in methicillin-resistant Staphylococcus aureus. For...

Characterization of new hydrophobic pili of human enterotoxigenic Escherichia coli: a possible new colonization factor.

Honda, T, Arita, M, Miwatani, T

Enterotoxigenic Escherichia coli strains were divided into five groups on the basis of their bacterial surface hydrophobicity (Honda et al., FEMS Microbiol. Lett. 17:273-276, 1983). Strains in group...

Evaluation in humans of attenuated Vibrio cholerae El Tor Ogawa strain Texas Star-SR as a live oral vaccine.

Levine, M M, Black, R E, Clements, M L, Lanata, C, Sears, S, Honda, T, ...

Texas Star-SR, an A- B+ mutant derived by nitrosoguanidine treatment from Vibrio cholerae El Tor Ogawa strain 3083, was fed to 68 volunteers as an oral vaccine in doses of 10(5) to 5 X 10(10)...

Effects of lincomycin and tetracycline on production and properties of enterotoxins of enterotoxigenic Escherichia coli.

Yoh, M, Yamamoto, K, Honda, T, Takeda, Y, Miwatani, T

Enterotoxigenic Escherichia coli grown in the presence of lincomycin and tetracycline produced an increased amount of heat-labile enterotoxin (LT). These antibiotics increased the production of not...

Analysis of antigenic determinants in cholera enterotoxin and heat-labile enterotoxins from human and porcine enterotoxigenic Escherichia coli.

Takeda, Y, Honda, T, Sima, H, Tsuji, T, Miwatani, T

Antigenic determinants of cholera enterotoxin (CT) and heat-labile enterotoxin from a human strain (LTh) and a porcine strain (LTp) were analyzed by Ouchterlony double-gel diffusion test against...

Enzyme-linked immunosorbent assay to detect Shiga toxin of Shigella dysenteriae and related toxins.

Kongmuang, U, Honda, T, Miwatani, T

A sandwich enzyme-linked immunosorbent assay (ELISA) was developed for detection of Shiga toxin. Four species of Shigella, Escherichia coli, and Vibro parahaemolyticus were tested for production of...

Evaluation of a nonisotopically labeled oligonucleotide probe to detect the heat-stable enterotoxin gene of Escherichia coli by the DNA colony hybridization test.

Nishibuchi, M, Arita, M, Honda, T, Miwatani, T

A commercially available, alkaline-phosphatase-conjugated oligonucleotide probe for detecting the heat-stable enterotoxin gene of Escherichia coli was compared with cloned gene probes by examining E....

Detection with synthetic oligonucleotide probes of nucleotide sequence variations in the genes encoding enterotoxins of Escherichia coli.

Nishibuchi, M, Murakami, A, Arita, M, Jikuya, H, Takano, J, Honda, T, ...

We examined variations in the genes encoding heat-stable enterotoxin (ST) and heat-labile enterotoxin (LT) in 88 strains of Escherichia coli isolated from individuals with traveler's diarrhea to find...

Enzyme-linked immunosorbent assays for detection of thermostable direct hemolysin of Vibrio parahaemolyticus.

Honda, T, Yoh, M, Kongmuang, U, Miwatani, T

Several systems for enzyme-linked immunosorbent assay (ELISA) of thermostable direct hemolysin (TDH) of Vibrio parahaemolyticus were tested, and single-antibody sandwich ELISA systems gave...

Comparison of preservation methods for enterotoxigenic Escherichia coli producing heat-labile enterotoxin.

Yoh, M, Narita, I, Honda, T, Miwatani, T, Nishibuchi, M

Ten strains of enterotoxigenic Escherichia coli producing heat-labile enterotoxin (LT) were preserved under 12 different conditions. After 1 month, 9 months, and 3 years of preservation, the cultures...

Differential detection of cholera enterotoxin and Escherichia coli heat-labile enterotoxin by enzyme-linked immunosorbent assays with antibodies specific to the two toxins.

Honda, T, Sato, M, Miwatani, T

Enzyme-linked immunosorbent assays (ELISAs) with antibodies specific to either cholera enterotoxin (CT) of Vibrio cholerae or heat-labile enterotoxin (LT) of enterotoxigenic Escherichia coli were...

Further evaluation of the Biken test (modified Elek test) for detection of enterotoxigenic Escherichia coli producing heat-labile enterotoxin and application of the test to sampling of heat-stable enterotoxin.

Honda, T, Arita, M, Takeda, Y, Miwatani, T

A total of 2,229 strains of Escherichia coli isolated from subjects with traveller's diarrhea were tested for heat-labile enterotoxin production by the Biken test (Honda et al., J. Clin. Microbiol....

Immunological detection of the Kanagawa phenomenon of vibrio parahaemolyticus on modified selective media.

Honda, T, Sornchai, C, Takeda, Y, Miwatani, T

Selective media for Vibrio parahaemolyticus, BTB-Teepol agar and modified arabinose-ammonium sulfate-cholate agar, were modified for use in immunological detection of the thermostable direct...

Detection by a staphylococcal coagglutination test of heat-labile enterotoxin-producing enterotoxigenic Escherichia coli.

Honda, T, Samakoses, R, Sornchai, C, Takeda, Y, Miwatani, T

For detection of heat-labile enterotoxin-producing enterotoxigenic Escherichia coli, the staphylococcal coagglutination test reported by Brill et al. (J. Clin. Microbiol. 9:49-55, 1979) was modified...

Immunological methods for detection of Kanagawa phenomenon of Vibrio parahaemolyticus.

Honda, T, Chearskul, S, Takeda, Y, Miwatani, T

A modified Elek test and an immuno-halo test were developed for detection of the thermostable direct hemolysin produced by Kanagawa phenomenon-positive strains of Vibrio parahaemolyticus. The...

Modified Elek test for detection of heat-labile enterotoxin of enterotoxigenic Escherichia coli.

Honda, T, Taga, S, Takeda, Y, Miwatani, T

The Elek test was modified for detection of the heat-labile enterotoxin of enterotoxigenic Escherichia coli. A total of 164 strains of E. coli were tested by the modified Elek test, and the results...

A mutant toxin of Vibrio parahaemolyticus thermostable direct hemolysin which has lost hemolytic activity but retains ability to bind to erythrocytes.

Tang, G Q, Iida, T, Yamamoto, K, Honda, T

A mutant toxin, R7, of thermostable direct hemolysin (TDH) with a single amino acid substitution at glycine 62 was analyzed. The hemolytic activity of R7 decreased to less than 1/1,000 of that of...

Effects of divalent cations and saccharides on Vibrio metschnikovii cytolysin-induced hemolysis of rabbit erythrocytes.

Miyake, M, Honda, T, Miwatani, T

Divalent cations and polysaccharides such as inulin and dextran reversibly inhibited hemolysis of rabbit erythrocytes caused by Vibrio metschnikovii cytolysin. On the basis of the 50% inhibitory...

Cloning and nucleotide sequence of the gene (trh) encoding the hemolysin related to the thermostable direct hemolysin of Vibrio parahaemolyticus.

Nishibuchi, M, Taniguchi, T, Misawa, T, Khaeomanee-Iam, V, Honda, T, Miwatani, T

Vibrio parahaemolyticus isolates derived from an outbreak of gastroenteritis in the Republic of Maldives did not have the genetic potential to produce the thermostable direct hemolysin, but one such...

Purification and characterization of a protease produced by Vibrio cholerae non-O1 and comparison with a protease of V. cholerae O1.

Honda, T, Lertpocasombat, K, Hata, A, Miwatani, T, Finkelstein, R A

A protease produced by a clinical isolate of Vibrio cholerae non-O1 was purified to apparent homogeneity by ammonium sulfate fractionation and successive column chromatography on DEAE-Sephadex A25,...

Two-step processing for activation of the cytolysin/hemolysin of Vibrio cholerae O1 biotype El Tor: nucleotide sequence of the structural gene (hlyA) and characterization of the processed products.

Yamamoto, K, Ichinose, Y, Shinagawa, H, Makino, K, Nakata, A, Iwanaga, M, ...

Vibrio cholerae O1 biotype El Tor produces and secretes a 65-kDa cytolysin/hemolysin into the culture medium. We cloned the structural gene (hlyA) for the cytolysin from the total DNA of a V....

Molecular heterogeneity of heat-labile enterotoxins from human and porcine enterotoxigenic Escherichia coli.

Tsuji, T, Taga, S, Honda, T, Takeda, Y, Miwatani, T

The heat-labile enterotoxins produced by human enterotoxigenic Escherichia coli (LTh) and porcine enterotoxigenic E. coli (LTp) were purified to homogeneity, and their molecular properties were...

Isolation of special antibodies which react only with homologous enterotoxins from Vibrio cholerae and Enterotoxigenic Escherichia coli.

Honda, T, Takeda, Y, Miwatani, T

Cholera enterotoxin (CT) and heat-labile enterotoxin (LT) produced by enterotoxigenic Escherichia coli share a common antigenic determinant. In addition, CT and LT each have a unique antigenic...

Immunological nonidentity of heat-labile enterotoxins from human and porcine enterotoxigenic Escherichia coli.

Honda, T, Tsuji, T, Takeda, Y, Miwatani, T

The Ouchterlony double-gel diffusion test demonstrated that heat-labile enterotoxin (LT) from human strains of enterotoxigenic Escherichia coli was antigenically related but not identical to that...

In vitro formation of hybrid toxins between subunits of Escherichia coli heat-labile enterotoxin and those of cholera enterotoxin.

Takeda, Y, Honda, T, Taga, S, Miwatani, T

Heat-labile enterotoxin (LT) was purified from cells of enterotoxigenic Escherichia coli isolated from a patient with traveller's diarrhea. Purified LT was separated into A and B subunits by...

Failure of chlorpromazine to inhibit fluid accumulation caused by Escherichia coli heat-stable enterotoxin in suckling mice.

Takeda, T, Honda, T, Takeda, Y, Miwatani, T

We studied the effect of chlorpromazine on fluid accumulation caused by purified heat-stable enterotoxin (ST) from enterotoxigenic Escherichia coli in suckling mice. We found that chlorpromazine...

Selection and characteristics of a Vibrio cholerae mutant lacking the A (ADP-ribosylating) portion of the cholera enterotoxin.

Honda, T, Finkelstein, R A

After mutagenesis with nitrosoguanidine and selection by immuno-halo techniques, an avirulent mutant, designated Texas Star-SR, which produces no detectable A (active; ADP-ribosylating) region of the...

Purification and characterization of a hemolysin produced by Vibrio cholerae biotype El Tor: another toxic substance produced by cholera vibrios.

Honda, T, Finkelstein, R A

A thermolabile direct hemolysin from an El Tor cholera vibrio strain has been isolated and partially characterized as a simple protein of ca. 20,000 molecular weight. In addition to its hemolytic...

Inhibition of hemolytic activity of the thermostable direct hemolysin of Vibrio parahaemolyticus by ganglioside.

Takeda, R, Honda, T, Sakural, J, Otomo, N

The hemolytic activity of the thermostable direct hemolysin produced by Vibrio parahaemolyticus was inhibited by a ganglioside mixture. The ganglioside component which inhibited the hemolysin was not...

Isolation of a factor causing morphological changes of chinese hamster ovary cells from the culture filtrate of Vibrio parahaemolyticus.

Honda, T, Shimizu, M, Takeda, Y, Miwatani, T

A factor changing Chinese hamster ovary cells from an oval to a spindle shape was isolated from the culture filtrate of Vibrio parahaemolyticus. It was partially purified by successive column...

Identification of lethal toxin with the thermostable direct hemolysin produced by Vibrio parahaemolyticus, and some physicochemical properties of the purified toxin.

Honda, T, Taga, S, Takeda, T, Hasibuan, M A, Takeda, Y, Miwatani, T

Lethal toxin was purified extensively from the culture filtrate of a Kanagawa phenomenon-positive strain of Vibrio parahaemolyticus. The purified toxin was a protein, and its homogeneity was...

Demonstration of the cardiotoxicity of the thermostable direct hemolysin (lethal toxin) produced by Vibrio parahaemolyticus.

Honda, T, Goshima, K, Takeda, Y, Sugino, Y, Miwatani, T

Intravenous injection of the thermostable direct hemolysin (lethal toxin) produced by Vibrio parahaemolyticus caused rapid death of rats. Studies by electroencephalography and electrocardiography...

Cytotoxic effect of the thermostable direct hemolysin produced by Vibrio parahaemolyticus on FL cells.

Sakurai, J, Honda, T, Jinguji, Y, Arita, M, Miwatani, T

The thermostable direct hemolysin produced by Vibrio parahaemolyticus showed cytotoxic activity on FL cells derived from human amniotic membrane. Scanning electron micrographs of the whole cells...

Inactivation of the biological activities of the thermostable direct hemolysin of Vibrio parahaemolyticus by ganglioside Gt1.

Takeda, Y, Takeda, T, Honda, T, Miwatani, T

Biological activities of the thermostable direct hemolysin produced by Vibrio parahaemolyticus, such as its hemolytic activity and lethal activity, were inhibited by neuraminidase-sensitive...

Endoscopic treatment of early oesophageal or gastric cancer.

Takeshita, K, Tani, M, Inoue, H, Saeki, I, Hayashi, S, Honda, T, ...

BACKGROUND AND AIMS: Endoscopic treatment has become increasingly popular in recent years as an alternative to surgical treatment with the hope of offering superior quality of life (QOL) for the...

Liver dysfunction among workers handling 5-nitro-o-toluidine

Shimizu, H, Kumada, T, Nakano, S, Kiriyama, S, Sone, Y, Honda, T, ...

Background: 5-Nitro-o-toluidine is an aromatic nitro amino compound. While other aromatic compounds are known to damage the human liver and are registered as toxic substances, toxicity information...

Effect of periodontal treatment on the serum antibody levels to heat shock proteins

YAMAZAKI, K, UEKI-MARUAYAMA, K, HONDA, T, NAKAJIMA, T, SEYMOUR, G J

We have shown previously that both humoral and cellular immune responses to heat shock protein 60 (HSP60) are elevated in chronic periodontitis patients compared with non-diseased subjects. The aim...

Balance of inflammatory response in stable gingivitis and progressive periodontitis lesions

Honda, T, Domon, H, Okui, T, Kajita, K, Amanuma, R, Yamazaki, K

The balance between inflammatory mediators and their counter-regulatory molecules may be crucial for determining the outcome of immune pathology of periodontal diseases. Based on clinical and...

Regulation of macrophage migration inhibitory factor (MIF) expression by glucose and insulin in adipocytes in vitro.

Sakaue, S., Nishihira, J., Hirokawa, J., Yoshimura, H., Honda, T., Aoki, K., ...

BACKGROUND: It has been reported that macrophage migration inhibitory factor (MIF) stimulated insulin secretion from pancreatic islet beta-cells in an autocrine manner, which suggests its pivotal...

Cytokines in bronchoalveolar lavage fluid in patients with high altitude pulmonary oedema at moderate altitude in Japan.

Kubo, K., Hanaoka, M., Yamaguchi, S., Hayano, T., Hayasaka, M., Koizumi, T., ...

BACKGROUND: The precise mechanism of high altitude pulmonary oedema (HAPE) remains unclear. The purpose of this study was to evaluate the role of cytokines and P-selectin in the development of HAPE...

Bronchoalveolar lavage fluid findings in patients with chronic hepatitis C virus infection.

Kubo, K, Yamaguchi, S, Fujimoto, K, Hanaoka, M, Hayasaka, M, Honda, T, ...

BACKGROUND: Hepatitis C virus (HCV) infection has recently been incriminated as an aetiological agent in idiopathic pulmonary fibrosis. This study was performed to determine the cellularity and...

Analysis of bronchoalveolar lavage fluid in patients with chronic hepatitis C before and after treatment with interferon alpha

Yamaguchi, S., Kubo, K., Fujimoto, K., Honda, T., Sekiguchi, M., Sodeyama, T.

BACKGROUND: Previous studies have shown that patients with idiopathic pulmonary fibrosis (IPF) were more likely to be seropositive for hepatitis C virus (HCV) than normal controls, and that patients...

Results of Endoscopic Treatment for Early Gastric Cancer by Nd-YAG Laser

Tani, M., Takeshita, K., Honda, T., Saito, N., Endo, M.

We have introduced two endoscopic treatments for early gastric cancer: endoscopic mucosal resection using a cap-fitted panendoscope (EMRC), and endoscopic laser therapy using a Nd-YAG laser....

Relationship of periodontal infection to serum antibody levels to periodontopathic bacteria and inflammatory markers in periodontitis patients with coronary heart disease

Yamazaki, K, Honda, T, Domon, H, Okui, T, Kajita, K, Amanuma, R, ...

Several reports have demonstrated a possible association of periodontal infections with coronary heart disease (CHD) by elevated antibody titre to periodontopathic bacteria in CHD patients compared...

Multidrug-resistant enteroaggregative Escherichia coli associated with persistent diarrhea in Kenyan children.

Sang, W. K., Oundo, J. O., Mwituria, J. K., Waiyaki, P. G., Yoh, M., Iida, T., ...

To study the association of multidrug-resistant enteroaggregative Escherichia coli with persistent diarrhea in Kenyan children, stool specimens were obtained from 862 outpatients under 5 years of age...

Filamentous phage associated with recent pandemic strains of Vibrio parahaemolyticus.

Iida, T., Hattori, A., Tagomori, K., Nasu, H., Naim, R., Honda, T.

A group of pandemic strains of Vibrio parahaemolyticus has recently appeared in Asia and North America. We demonstrate that a filamentous phage is specifically associated with the pandemic V....

RASSF2, a potential tumour suppressor, is silenced by CpG island hypermethylation in gastric cancer

Endoh, M, Tamura, G, Honda, T, Homma, N, Terashima, M, Nishizuka, S, ...

RASSF2, a member of the RASSF1 family, has recently been identified as a potential tumour suppressor. We examined methylation status in multiple regions which included the CpG island and spanned the...

Cisplatin-incorporating polymeric micelles (NC-6004) can reduce nephrotoxicity and neurotoxicity of cisplatin in rats

Uchino, H, Matsumura, Y, Negishi, T, Koizumi, F, Hayashi, T, Honda, T, ...

In spite of the clinical usefulness of cisplatin (CDDP), there are many occasions in which it is difficult to continue the administration of CDDP due to its nephrotoxicity and neurotoxicity. We...

Results of three-year mass screening programme for lung cancer using mobile low-dose spiral computed tomography scanner

Sone, S, Li, F, Yang, Z-G, Honda, T, Maruyama, Y, Takashima, S, ...

The aim of this study was to evaluate the usefulness of annual screening for lung cancer by low-dose computed tomography (CT) and the characteristics of identified lung cancers. Subjects consisted of...