Bottomley, K. M., Borkakoti, N., Bradshaw, D., Brown, P. A., Broadhurst, M. J., Budd, J. M., ...
N-terminal analysis of aggrecan fragments lost from bovine nasal cartilage cultured in the presence of recombinant human interleukin 1alpha revealed a predominant ARGSVIL sequence with an additional...
Jackson, T R, Hallam, T J, Downes, C P, Hanley, M R
The addition of bradykinin to NG115-401L cells grown on coverslips results in the generation of rapid transient increases in intracellular [Ca2+] and inositol phosphates. Changes in intracellular...
A role for calcium and protein kinase C in agonist-stimulated adhesion of human neutrophils.
Davies, M P, Hallam, T J, Merritt, J E
Stimulated adherence of human neutrophils to plastic and changes in cytosolic free Ca2+ concn. [( Ca2+]i) were measured in the same cell preparations. [Ca2+]i-activation curves were constructed to...
Carter, T D, Hallam, T J, Pearson, J D
Agonist-stimulated release of prostacyclin (PGI2) from endothelial cells requires elevation of the concentration of intracellular ionized calcium ([Ca2+]i) above a threshold value, and raised [Ca2+]i...
McCarthy, S A, Hallam, T J, Merritt, J E
Stimulation of fura-2-loaded human neutrophils with formylmethionyl-leucyl-phenylalanine (FMLP) or ionomycin elevated the cytosolic free Ca2+ concentration, [Ca2+], to a maintained elevated level....
Evidence that agonists stimulate bivalent-cation influx into human endothelial cells.
Hallam, T J, Jacob, R, Merritt, J E
Human umbilical-vein endothelial cells stimulated with thrombin or histamine show an increase in [Ca2+]i (cytoplasmic free calcium concn.) that is maintained well above the basal pre-stimulated value...
Conroy, L A, Merritt, J E, Hallam, T J
Stimulation of Jurkat E6 cells with anti-CD3 antibody results in a characteristic rise in [Ca2+]i which is due to both the release of Ca2+ from intracellular stores and the entry of external Ca2+....
Sage, S O, Merritt, J E, Hallam, T J, Rink, T J
Previous studies of the early kinetics of rises in cytosolic free [Ca2+] in fura-2-loaded human platelets suggested that: (1) Ca2+ entry slightly preceded internal discharge with thrombin and other...
Influx of bivalent cations can be independent of receptor stimulation in human endothelial cells.
Hallam, T J, Jacob, R, Merritt, J E
Stimulation of human umbilical-vein endothelial cells by agonists such as histamine or thrombin promotes an influx of Ca2+, causing an increase in cytoplasmic free Ca2+ ([Ca2+]i) that is dependent on...
Hallam, T J, Thompson, N T, Scrutton, M C, Rink, T J
Responses to vasopressin were studied in human platelets loaded with the fluorescent Ca2+ indicator, quin2. In the presence of 1 mM external Ca2+, vasopressin caused a transient rise in [Ca2+]i from...
Thyrotropin-releasing hormone activates Na+/H+ exchange in rat pituitary cells.
The effects of thyrotropin-releasing hormone (TRH) and 12-O-tetradecanoylphorbol 13-acetate (TPA) on cytosolic pH (pHi) were studied on GH4C1 pituitary cells loaded with the fluorescent pH indicator...
Hallam, T J, Pearson, J D, Needham, L A
Endothelial cells are known to release prostacyclin (PGI2) in response to agonists, and this has generally been assumed to be caused, at least in part, by activation of a phospholipase A2 by elevated...
SK&F 96365, a novel inhibitor of receptor-mediated calcium entry.
Merritt, J E, Armstrong, W P, Benham, C D, Hallam, T J, Jacob, R, Jaxa-Chamiec, A, ...
A novel inhibitor of receptor-mediated calcium entry (RMCE) is described. SK&F 96365 (1-(beta-[3-(4-methoxy-phenyl)propoxy]-4-methoxyphenethyl)-1H- imidazole hydrochloride) is structurally distinct...
Hallam, T J, Daniel, J L, Kendrick-Jones, J, Rink, T J
Human platelets were prepared and loaded with the fluorescent Ca2+ indicator quin2. The relation between cytoplasmic free calcium concentration, [Ca2+]i, and the extent of the phosphorylation of...
Hallam, T J, Sanchez, A, Rink, T J
The role of changes in cytoplasmic free calcium, [Ca2+]i, in the responses to platelet-activating factor (PAF) was studied in human platelets loaded with the fluorescent calcium indicator, quin2. In...
Inhibition of bovine nasal cartilage degradation by selective matrix metalloproteinase inhibitors.
Bottomley, K M, Borkakoti, N, Bradshaw, D, Brown, P A, Broadhurst, M J, Budd, J M, ...
N-terminal analysis of aggrecan fragments lost from bovine nasal cartilage cultured in the presence of recombinant human interleukin 1alpha revealed a predominant ARGSVIL sequence with an additional...
ADP produces a rapid elevation in the concentration of cytoplasmic free calcium, [Ca2+]i, in quin2-loaded human platelets which begins within 1 s of stimulation and peaks after 10 s. In the presence...
Jackson, T R, Hallam, T J, Downes, C P, Hanley, M R
The addition of bradykinin to NG115-401L cells grown on coverslips results in the generation of rapid transient increases in intracellular [Ca2+] and inositol phosphates. Changes in intracellular...
A role for calcium and protein kinase C in agonist-stimulated adhesion of human neutrophils.
Davies, M P, Hallam, T J, Merritt, J E
Stimulated adherence of human neutrophils to plastic and changes in cytosolic free Ca2+ concn. [( Ca2+]i) were measured in the same cell preparations. [Ca2+]i-activation curves were constructed to...
Carter, T D, Hallam, T J, Pearson, J D
Agonist-stimulated release of prostacyclin (PGI2) from endothelial cells requires elevation of the concentration of intracellular ionized calcium ([Ca2+]i) above a threshold value, and raised [Ca2+]i...
McCarthy, S A, Hallam, T J, Merritt, J E
Stimulation of fura-2-loaded human neutrophils with formylmethionyl-leucyl-phenylalanine (FMLP) or ionomycin elevated the cytosolic free Ca2+ concentration, [Ca2+], to a maintained elevated level....
Evidence that agonists stimulate bivalent-cation influx into human endothelial cells.
Hallam, T J, Jacob, R, Merritt, J E
Human umbilical-vein endothelial cells stimulated with thrombin or histamine show an increase in [Ca2+]i (cytoplasmic free calcium concn.) that is maintained well above the basal pre-stimulated value...
Conroy, L A, Merritt, J E, Hallam, T J
Stimulation of Jurkat E6 cells with anti-CD3 antibody results in a characteristic rise in [Ca2+]i which is due to both the release of Ca2+ from intracellular stores and the entry of external Ca2+....
Sage, S O, Merritt, J E, Hallam, T J, Rink, T J
Previous studies of the early kinetics of rises in cytosolic free [Ca2+] in fura-2-loaded human platelets suggested that: (1) Ca2+ entry slightly preceded internal discharge with thrombin and other...
Influx of bivalent cations can be independent of receptor stimulation in human endothelial cells.
Hallam, T J, Jacob, R, Merritt, J E
Stimulation of human umbilical-vein endothelial cells by agonists such as histamine or thrombin promotes an influx of Ca2+, causing an increase in cytoplasmic free Ca2+ ([Ca2+]i) that is dependent on...
Hallam, T J, Thompson, N T, Scrutton, M C, Rink, T J
Responses to vasopressin were studied in human platelets loaded with the fluorescent Ca2+ indicator, quin2. In the presence of 1 mM external Ca2+, vasopressin caused a transient rise in [Ca2+]i from...
Thyrotropin-releasing hormone activates Na+/H+ exchange in rat pituitary cells.
The effects of thyrotropin-releasing hormone (TRH) and 12-O-tetradecanoylphorbol 13-acetate (TPA) on cytosolic pH (pHi) were studied on GH4C1 pituitary cells loaded with the fluorescent pH indicator...
Hallam, T J, Pearson, J D, Needham, L A
Endothelial cells are known to release prostacyclin (PGI2) in response to agonists, and this has generally been assumed to be caused, at least in part, by activation of a phospholipase A2 by elevated...
SK&F 96365, a novel inhibitor of receptor-mediated calcium entry.
Merritt, J E, Armstrong, W P, Benham, C D, Hallam, T J, Jacob, R, Jaxa-Chamiec, A, ...
A novel inhibitor of receptor-mediated calcium entry (RMCE) is described. SK&F 96365 (1-(beta-[3-(4-methoxy-phenyl)propoxy]-4-methoxyphenethyl)-1H- imidazole hydrochloride) is structurally distinct...
Hallam, T J, Daniel, J L, Kendrick-Jones, J, Rink, T J
Human platelets were prepared and loaded with the fluorescent Ca2+ indicator quin2. The relation between cytoplasmic free calcium concentration, [Ca2+]i, and the extent of the phosphorylation of...
Hallam, T J, Sanchez, A, Rink, T J
The role of changes in cytoplasmic free calcium, [Ca2+]i, in the responses to platelet-activating factor (PAF) was studied in human platelets loaded with the fluorescent calcium indicator, quin2. In...
ADP produces a rapid elevation in the concentration of cytoplasmic free calcium, [Ca2+]i, in quin2-loaded human platelets which begins within 1 s of stimulation and peaks after 10 s. In the presence...
Inhibition of bovine nasal cartilage degradation by selective matrix metalloproteinase inhibitors.
Bottomley, K M, Borkakoti, N, Bradshaw, D, Brown, P A, Broadhurst, M J, Budd, J M, ...
N-terminal analysis of aggrecan fragments lost from bovine nasal cartilage cultured in the presence of recombinant human interleukin 1alpha revealed a predominant ARGSVIL sequence with an additional...
Carter, T. D., Hallam, T. J., Cusack, N. J., Pearson, J. D.
1. ATP and ATP analogues induced prostacyclin (PGI2) secretion from human cultured umbilical vein endothelial cells. 2. The threshold active concentration for ATP was less than or equal to 1 microM....
The effects of siguazodan, a selective phosphodiesterase inhibitor, on human platelet function.
Murray, K. J., England, P. J., Hallam, T. J., Maguire, J., Moores, K., Reeves, M. L., ...
1. The effects of siguazodan (SK&F 94836) a selective phosphodiesterase (PDE) inhibitor with inotropic and vasodilator activity, were studied on human platelets. 2. Siguazodan selectively inhibited...