Scientific Commons: T. J. Kunicki

Defective dimerization of von Willebrand factor subunits due to a Cys-> Arg mutation in type IID von Willebrand disease.

Schneppenheim, R, Brassard, J, Krey, S, Budde, U, Kunicki, T J, Holmberg, L, ...

The same heterozygous T -> C transition at nt 8567 of the von Willebrand factor (vWF) transcript was found in two unrelated patients with type III) von Willebrand disease, with no other apparent...

Localization of human platelet autoantigens to the cysteine-rich region of glycoprotein IIIa.

Kekomaki, R, Dawson, B, McFarland, J, Kunicki, T J

The object of this study was to further localize autoantigenic structures on IIb-IIIa and, if possible, to precisely identify the epitopes recognized by human autoantibodies. In this paper, we...

Surface modifications in the platelets of a patient with alpha-N-acetyl-D-galactosamine residues, the Tn-syndrome.

Nurden, A T, Dupuis, D, Pidard, D, Kieffer, N, Kunicki, T J, Cartron, J P

The Tn-syndrome is an acquired disorder characterized by the polyagglutination of blood cells and the pathological exposure of alpha-N-acetyl-D-galactosamine residues (Tn-antigen) at the cell...

Inheritance of the human platelet alloantigen, PlA1, in type I Glanzmann's thrombasthenia.

Kunicki, T J, Pidard, D, Cazenave, J P, Nurden, A T, Caen, J P

The hereditary of the human platelet alloantigen, PlA1, has been studied in Glanzmann's thrombasthenia. The PlA1 content of platelets from three patients, 20 kindred of these patients, including...

Analysis of the glycoprotein and protein composition of Bernard-Soulier platelets by single and two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

Nurden, A T, Dupuis, D, Kunicki, T J, Caen, J P

Previous reports have described conflicting results concerning the glycoprotein (GP) and protein composition of Bernard-Soulier platelets. In view of this controversy we have analyzed the platelets...

Absence of the platelet receptor for drug-dependent antibodies in the Bernard-Soulier syndrome.

Kunicki, T J, Johnson, M M, Aster, R H

The platelet membrane receptor for quinidine- and quinine-dependent antibodies was studied in three patients with the Bernard-Soulier syndrome (BSS) and in normal subjects with immunologic techniques...

Synthesis of analogs of human platelet membrane glycoprotein IIb-IIIa complex by chicken peripheral blood thrombocytes.

Kunicki, T J, Newman, P J

Human platelets and their phylogenetic counterparts, avian thrombocytes, play a key role in primary hemostasis. Based upon extensive studies in mammals, platelet cohesion resulting in the formation...

Platelet surface glycoproteins. Studies on resting and activated platelets and platelet membrane microparticles in normal subjects, and observations in patients during adult respiratory distress syndrome and cardiac surgery.

George, J N, Pickett, E B, Saucerman, S, McEver, R P, Kunicki, T J, Kieffer, N, ...

The accurate definition of surface glycoprotein abnormalities in circulating platelets may provide better understanding of bleeding and thrombotic disorders. Platelet surface glycoproteins were...

Divalent cation regulation of the surface orientation of platelet membrane glycoprotein IIb. Correlation with fibrinogen binding function and definition of a novel variant of Glanzmann's thrombasthenia.

Ginsberg, M H, Lightsey, A, Kunicki, T J, Kaufmann, A, Marguerie, G, Plow, E F

An antiplatelet monoclonal antibody, PMI-1, reacts with glycoproteins (GP) GPIIb, free GPIIb, and the GPIIb-IIIa complex. This antibody binds to 40,900 sites per platelet, with a Kd = 0.95 microM,...

Independent modulation of von Willebrand factor and fibrinogen binding to the platelet membrane glycoprotein IIb/IIIa complex as demonstrated by monoclonal antibody.

Lombardo, V T, Hodson, E, Roberts, J R, Kunicki, T J, Zimmerman, T S, Ruggeri, Z M

In this study we have used two new monoclonal antibodies, designated LJP5 and LJP9, as well as a previously described one, AP2, all specific for the platelet membrane glycoprotein (GP)IIb/IIIa...

Diagnosis of Bernard-Soulier syndrome and Glanzmann's thrombasthenia with a monoclonal assay on whole blood.

Montgomery, R R, Kunicki, T J, Taves, C, Pidard, D, Corcoran, M

Two hereditary platelet disorders, Bernard-Soulier syndrome and Glanzmann's thrombasthenia, are characterized by selective deficiencies of platelet membrane glycoproteins. Murine monoclonal...

On the association of the platelet-specific alloantigen, Pena, with glycoprotein IIIa. Evidence for heterogeneity of glycoprotein IIIa.

Furihata, K, Nugent, D J, Bissonette, A, Aster, R H, Kunicki, T J

Neonatal alloimmune thrombocytopenic purpura associated with a new platelet-specific alloantigen Pena has been reported. We now provide direct evidence that the Pena determinant is associated with...

Effects of monoclonal antibodies against the platelet glycoprotein IIb/IIIa complex on thrombosis and hemostasis in the baboon.

Hanson, S R, Pareti, F I, Ruggeri, Z M, Marzec, U M, Kunicki, T J, Montgomery, R R, ...

To assess the hemostatic consequences and antithrombotic effectiveness of blocking the platelet glycoprotein (GP) IIb/IIIa receptor for fibrinogen and other adhesive glycoproteins in vivo, well...

Immunological characterization of eristostatin and echistatin binding sites on alpha IIb beta 3 and alpha V beta 3 integrins.

Marcinkiewicz, C, Rosenthal, L A, Mosser, D M, Kunicki, T J, Niewiarowski, S

Two disintegrins with a high degree of amino acid sequence similarity, echistatin and eristostatin, showed a low level of interaction with Chinese hamster ovary (CHO) cells, but they bound to CHO...

Lateral diffusion of lipid probes in the surface membrane of human platelets. An electron-electron double resonance (ELDOR) study.

Lai, C S, Wirt, M D, Yin, J J, Froncisz, W, Feix, J B, Kunicki, T J, ...

Electron-electron double resonance (ELDOR) techniques employing [14N], [15N] 16-Doxylstearate spin-label pairs have been used to measure the lateral diffusion constant, D, of lipids in the surface...