T. J. Phelps

Survival of subsurface microorganisms exposed to UV radiation and hydrogen peroxide.

Arrage, A A, Phelps, T J, Benoit, R E, White, D C

Aerobic and microaerophilic subsurface bacteria were screened for resistance to UV light. Contrary to the hypothesis that subsurface bacteria should be sensitive to UV light, the organisms studied...

Aerobic mineralization of vinyl chloride by a bacterium of the order Actinomycetales.

Phelps, T J, Malachowsky, K, Schram, R M, White, D C

A gram-positive branched bacterium isolated from a trichloroethylene-degrading consortium mineralized vinyl chloride in growing cultures and cell suspensions. Greater than 67% of the [1,2-14C]vinyl...

Biodegradation of Trichloroethylene in Continuous-Recycle Expanded-Bed Bioreactors

Phelps, T. J., Niedzielski, J. J., Schram, R. M., Herbes, S. E., White, D. C.

Experimental bioreactors operated as recirculated closed systems were inoculated with bacterial cultures that utilized methane, propane, and tryptone-yeast extract as aerobic carbon and energy...

Aerobic Mineralization of Trichloroethylene, Vinyl Chloride, and Aromatic Compounds by Rhodococcus Species

Malachowsky, K. J., Phelps, T. J., Teboli, A. B., Minnikin, D. E., White, D. C.

Two Rhodococcus strains which were isolated from a trichloroethylene (TCE)-degrading bacterial mixture and Rhodococcus rhodochrous ATCC 21197 mineralized vinyl chloride (VC) and TCE. Greater than...

Mineralization of Trichloroethylene by Heterotrophic Enrichment Cultures

Fliermans, C. B., Phelps, T. J., Ringelberg, D., Mikell, A. T., White, D. C.

Microbial consortia capable of aerobically degrading more than 99% of exogenous trichloroethylene (TCE) (50 mg/liter) were collected from TCE-contaminated subsurface sediments and grown in enrichment...

Sulfate-Dependent Interspecies H2 Transfer between Methanosarcina barkeri and Desulfovibrio vulgaris during Coculture Metabolism of Acetate or Methanol

Phelps, T. J., Conrad, R., Zeikus, J. G.

We compared the metabolism of methanol and acetate when Methanosarcina barkeri was grown in the presence and absence of Desulfovibrio vulgaris. The sulfate reducer was not able to utilize methanol or...

Gas Metabolism Evidence in Support of the Juxtaposition of Hydrogen-Producing and Methanogenic Bacteria in Sewage Sludge and Lake Sediments

Conrad, R., Phelps, T. J., Zeikus, J. G.

We developed new techniques to measure dissolved H2 and H2 consumption kinetics in anoxic ecosystems that were not dependent on headspace measurements or gas transfer-limited experimentation. These...

Effect of Fall Turnover on Terminal Carbon Metabolism in Lake Mendota Sediments

Phelps, T. J., Zeikus, J. G.

The carbon and electron flow pathways and the bacterial populations responsible for the transformation of H2-CO2, formate, methanol, methylamine, acetate, ethanol, and lactate were examined in...

Influence of pH on Terminal Carbon Metabolism in Anoxic Sediments from a Mildly Acidic Lake

Phelps, T. J., Zeikus, J. G.

The carbon and electron flow pathways and the bacterial populations responsible for transformation of H2-CO2, formate, methanol, methylamine, acetate, glycine, ethanol, and lactate were examined in...

Microbiological Comparisons within and across Contiguous Lacustrine, Paleosol, and Fluvial Subsurface Sediments

Kieft, T. L., Fredrickson, J. K., McKinley, J. P., Bjornstad, B. N., Rawson, S. A., Phelps, T. J., ...

Twenty-six subsurface samples were collected from a borehole at depths of 173.3 to 196.8 m in the saturated zone at the Hanford Site in south-central Washington State. The sampling was performed...

Survival of subsurface microorganisms exposed to UV radiation and hydrogen peroxide.

Arrage, A A, Phelps, T J, Benoit, R E, White, D C

Aerobic and microaerophilic subsurface bacteria were screened for resistance to UV light. Contrary to the hypothesis that subsurface bacteria should be sensitive to UV light, the organisms studied...

Aerobic mineralization of vinyl chloride by a bacterium of the order Actinomycetales.

Phelps, T J, Malachowsky, K, Schram, R M, White, D C

A gram-positive branched bacterium isolated from a trichloroethylene-degrading consortium mineralized vinyl chloride in growing cultures and cell suspensions. Greater than 67% of the [1,2-14C]vinyl...

Biodegradation of Trichloroethylene in Continuous-Recycle Expanded-Bed Bioreactors

Phelps, T. J., Niedzielski, J. J., Schram, R. M., Herbes, S. E., White, D. C.

Experimental bioreactors operated as recirculated closed systems were inoculated with bacterial cultures that utilized methane, propane, and tryptone-yeast extract as aerobic carbon and energy...

Aerobic Mineralization of Trichloroethylene, Vinyl Chloride, and Aromatic Compounds by Rhodococcus Species

Malachowsky, K. J., Phelps, T. J., Teboli, A. B., Minnikin, D. E., White, D. C.

Two Rhodococcus strains which were isolated from a trichloroethylene (TCE)-degrading bacterial mixture and Rhodococcus rhodochrous ATCC 21197 mineralized vinyl chloride (VC) and TCE. Greater than...

Mineralization of Trichloroethylene by Heterotrophic Enrichment Cultures

Fliermans, C. B., Phelps, T. J., Ringelberg, D., Mikell, A. T., White, D. C.

Microbial consortia capable of aerobically degrading more than 99% of exogenous trichloroethylene (TCE) (50 mg/liter) were collected from TCE-contaminated subsurface sediments and grown in enrichment...

Sulfate-Dependent Interspecies H2 Transfer between Methanosarcina barkeri and Desulfovibrio vulgaris during Coculture Metabolism of Acetate or Methanol

Phelps, T. J., Conrad, R., Zeikus, J. G.

We compared the metabolism of methanol and acetate when Methanosarcina barkeri was grown in the presence and absence of Desulfovibrio vulgaris. The sulfate reducer was not able to utilize methanol or...

Gas Metabolism Evidence in Support of the Juxtaposition of Hydrogen-Producing and Methanogenic Bacteria in Sewage Sludge and Lake Sediments

Conrad, R., Phelps, T. J., Zeikus, J. G.

We developed new techniques to measure dissolved H2 and H2 consumption kinetics in anoxic ecosystems that were not dependent on headspace measurements or gas transfer-limited experimentation. These...

Effect of Fall Turnover on Terminal Carbon Metabolism in Lake Mendota Sediments

Phelps, T. J., Zeikus, J. G.

The carbon and electron flow pathways and the bacterial populations responsible for the transformation of H2-CO2, formate, methanol, methylamine, acetate, ethanol, and lactate were examined in...

Influence of pH on Terminal Carbon Metabolism in Anoxic Sediments from a Mildly Acidic Lake

Phelps, T. J., Zeikus, J. G.

The carbon and electron flow pathways and the bacterial populations responsible for transformation of H2-CO2, formate, methanol, methylamine, acetate, glycine, ethanol, and lactate were examined in...

Microbiological Comparisons within and across Contiguous Lacustrine, Paleosol, and Fluvial Subsurface Sediments

Kieft, T. L., Fredrickson, J. K., McKinley, J. P., Bjornstad, B. N., Rawson, S. A., Phelps, T. J., ...

Twenty-six subsurface samples were collected from a borehole at depths of 173.3 to 196.8 m in the saturated zone at the Hanford Site in south-central Washington State. The sampling was performed...

Estimates of Biogenic Methane Production Rates in Deep Marine Sediments at Hydrate Ridge, Cascadia Margin ▿ †

Colwell, F. S., Boyd, S., Delwiche, M. E., Reed, D. W., Phelps, T. J., Newby, D. T.

Methane hydrate found in marine sediments is thought to contain gigaton quantities of methane and is considered an important potential fuel source and climate-forcing agent. Much of the methane in...