Hepatitis B Virus X Protein Is both a Substrate and a Potential Inhibitor of the Proteasome Complex
Hu, Zongyi, Zhang, Zhensheng, Doo, Edward, Coux, Olivier, Goldberg, Alfred L., Liang, T. Jake
The hepatitis B virus X protein (HBX) is essential for the establishment of HBV infection in vivo and exerts a pleiotropic effect on diverse cellular functions. The yeast two-hybrid system had...
Hepatitis C Virus Structural Proteins Assemble into Viruslike Particles in Insect Cells
Baumert, Thomas F., Ito, Susumu, Wong, David T., Liang, T. Jake
Hepatitis C virus (HCV) is a leading cause of chronic hepatitis in the world. The study of HCV has been hampered by the low level of viral particles in infected individuals, the inability to...
Baumert, Thomas F., Marrone, Aldo, Vergalla, John, Liang, T. Jake
Functional analysis of naturally occurring hepatitis B virus (HBV) mutations is crucial in understanding their impact on disease. We have recently identified two mutations in the HBV core promoter of...
Satoi, Jujin, Murata, Kazumoto, Lechmann, Martin, Manickan, Elanchezhiyan, Zhang, Zhensheng, Wedemeyer, Heiner, ...
To study the effect of genetic immunization on transgenic expression of hepatitis C virus (HCV) proteins, we evaluated the immunological response of HCV transgenic mice to HCV expression plasmids....
Mizukoshi, Eishiro, Nascimbeni, Michelina, Blaustein, Joshua B., Mihalik, Kathleen, Rice, Charles M., Liang, T. Jake, ...
The chimpanzee is a critical animal model for studying cellular immune responses to infectious pathogens such as hepatitis B and C viruses, human immunodeficiency virus, and malaria. Several...
Triyatni, Miriam, Saunier, Bertrand, Maruvada, Padma, Davis, Anthony R., Ulianich, Luca, Heller, Theo, ...
Hepatitis C virus-like particles (HCV-LPs) containing the structural proteins of HCV H77 strain (1a genotype) was used as a model for HCV virion to study virus-cell interaction. HCV-LPs showed a...
Thomson, Michael, Nascimbeni, Michelina, Havert, Michael B., Major, Marian, Gonzales, Sophia, Alter, Harvey, ...
Clearance of hepatitis C virus (HCV) infection in humans and chimpanzees is thought to be associated with the induction of strong T-cell responses. We studied four chimpanzees infected with HCV...
Murata, Kazumoto, Lechmann, Martin, Qiao, Ming, Gunji, Toshiaki, Alter, Harvey J., Liang, T. Jake
We have recently demonstrated that immunization with hepatitis C virus-like particles (HCV-LPs) generated in insect cells can elicit both humoral and cellular immune responses in BALB/c mice. Here,...
Zhang, Zhensheng, Torii, Nobuyuki, Hu, Zongyi, Jacob, James, Liang, T. Jake
The X protein (HBX) of the hepatitis B virus (HBV) has been shown to be important for the establishment of HBV infection in vivo. Our previous studies suggested that interaction of HBX with the...
Effects of Mutations of the Initiation Nucleotides on Hepatitis C Virus RNA Replication in the Cell
Cai, Zhaohui, Liang, T. Jake, Luo, Guangxiang
Replication of nearly all RNA viruses depends on a virus-encoded RNA-dependent RNA polymerase (RdRp). Our earlier work found that purified recombinant hepatitis C virus (HCV) RdRp (NS5B) was able to...
Zhang, Zhensheng, Protzer, Ulrike, Hu, Zongyi, Jacob, James, Liang, T. Jake
The X protein (HBX) of the hepatitis B virus (HBV) is not essential for the HBV life cycle in vitro but is important for productive infection in vivo. Our previous study suggests that interaction of...
Keck, Zhen-Yong, Sung, Vicky M. H., Perkins, Susan, Rowe, Judy, Paul, Sudhir, Liang, T. Jake, ...
Human antibodies elicited in response to hepatitis C virus (HCV) infection are anticipated to react with the native conformation of the viral envelope structure. Isolation of these antibodies as...
Jeong, Sook-Hyang, Qiao, Ming, Nascimbeni, Michelina, Hu, Zongyi, Rehermann, Barbara, Murthy, Krishna, ...
We have previously reported the production of hepatitis C virus-like particles (HCV-LP) using a recombinant baculovirus containing the cDNA of the HCV structural proteins (core, E1, and E2). These...
Steinmann, Daniel, Barth, Heidi, Gissler, Bettina, Schürmann, Peter, Adah, Mohammed I., Gerlach, J. Tilman, ...
Hepatitis C virus (HCV) is a leading cause of chronic viral hepatitis worldwide. The study of antibody-mediated virus neutralization has been hampered by the lack of an efficient and high-throughput...
An in vitro model of hepatitis C virion production
Heller, Theo, Saito, Satoru, Auerbach, Jonathan, Williams, Tarice, Moreen, Tzivia Rachel, Jazwinski, Allison, ...
The hepatitis C virus (HCV) is a major cause of liver disease worldwide. The understanding of the viral life cycle has been hampered by the lack of a satisfactory cell culture system. The development...
Radaeva, Svetlana, Jaruga, Barbara, Kim, Won-Ho, Heller, Theo, Liang, T Jake, Gao, Bin
IFN-gamma (interferon-gamma) modulates IFN-alpha therapy in chronic hepatitis C infection; however, the underlying mechanism remains unclear. Here we demonstrate that long-term (3-6 days) but not...
Cai, Zhaohui, Zhang, Chen, Chang, Kyung-Soo, Jiang, Jieyun, Ahn, Byung-Chul, Wakita, Takaji, ...
Hepatitis C virus (HCV) chronically infects approximately 170 million people worldwide, with an increased risk of developing cirrhosis and hepatocellular carcinoma. The study of HCV replication and...
Altered Proteolysis and Global Gene Expression in Hepatitis B Virus X Transgenic Mouse Liver
Hu, Zongyi, Zhang, Zhensheng, Kim, Jin Woo, Huang, Ying, Liang, T. Jake
Hepatitis B virus X (HBX) is essential for the productive infection of hepatitis B virus (HBV) in vivo and has a pleiotropic effect on host cells. We have previously demonstrated that the proteasome...
Hepatitis B Virus X Protein Is both a Substrate and a Potential Inhibitor of the Proteasome Complex
Hu, Zongyi, Zhang, Zhensheng, Doo, Edward, Coux, Olivier, Goldberg, Alfred L., Liang, T. Jake
The hepatitis B virus X protein (HBX) is essential for the establishment of HBV infection in vivo and exerts a pleiotropic effect on diverse cellular functions. The yeast two-hybrid system had...
Hepatitis C Virus Structural Proteins Assemble into Viruslike Particles in Insect Cells
Baumert, Thomas F., Ito, Susumu, Wong, David T., Liang, T. Jake
Hepatitis C virus (HCV) is a leading cause of chronic hepatitis in the world. The study of HCV has been hampered by the low level of viral particles in infected individuals, the inability to...
Baumert, Thomas F., Marrone, Aldo, Vergalla, John, Liang, T. Jake
Functional analysis of naturally occurring hepatitis B virus (HBV) mutations is crucial in understanding their impact on disease. We have recently identified two mutations in the HBV core promoter of...
Satoi, Jujin, Murata, Kazumoto, Lechmann, Martin, Manickan, Elanchezhiyan, Zhang, Zhensheng, Wedemeyer, Heiner, ...
To study the effect of genetic immunization on transgenic expression of hepatitis C virus (HCV) proteins, we evaluated the immunological response of HCV transgenic mice to HCV expression plasmids....
Mizukoshi, Eishiro, Nascimbeni, Michelina, Blaustein, Joshua B., Mihalik, Kathleen, Rice, Charles M., Liang, T. Jake, ...
The chimpanzee is a critical animal model for studying cellular immune responses to infectious pathogens such as hepatitis B and C viruses, human immunodeficiency virus, and malaria. Several...
Triyatni, Miriam, Saunier, Bertrand, Maruvada, Padma, Davis, Anthony R., Ulianich, Luca, Heller, Theo, ...
Hepatitis C virus-like particles (HCV-LPs) containing the structural proteins of HCV H77 strain (1a genotype) was used as a model for HCV virion to study virus-cell interaction. HCV-LPs showed a...
Thomson, Michael, Nascimbeni, Michelina, Havert, Michael B., Major, Marian, Gonzales, Sophia, Alter, Harvey, ...
Clearance of hepatitis C virus (HCV) infection in humans and chimpanzees is thought to be associated with the induction of strong T-cell responses. We studied four chimpanzees infected with HCV...
Murata, Kazumoto, Lechmann, Martin, Qiao, Ming, Gunji, Toshiaki, Alter, Harvey J., Liang, T. Jake
We have recently demonstrated that immunization with hepatitis C virus-like particles (HCV-LPs) generated in insect cells can elicit both humoral and cellular immune responses in BALB/c mice. Here,...
Zhang, Zhensheng, Torii, Nobuyuki, Hu, Zongyi, Jacob, James, Liang, T. Jake
The X protein (HBX) of the hepatitis B virus (HBV) has been shown to be important for the establishment of HBV infection in vivo. Our previous studies suggested that interaction of HBX with the...
Effects of Mutations of the Initiation Nucleotides on Hepatitis C Virus RNA Replication in the Cell
Cai, Zhaohui, Liang, T. Jake, Luo, Guangxiang
Replication of nearly all RNA viruses depends on a virus-encoded RNA-dependent RNA polymerase (RdRp). Our earlier work found that purified recombinant hepatitis C virus (HCV) RdRp (NS5B) was able to...
Zhang, Zhensheng, Protzer, Ulrike, Hu, Zongyi, Jacob, James, Liang, T. Jake
The X protein (HBX) of the hepatitis B virus (HBV) is not essential for the HBV life cycle in vitro but is important for productive infection in vivo. Our previous study suggests that interaction of...
Keck, Zhen-Yong, Sung, Vicky M. H., Perkins, Susan, Rowe, Judy, Paul, Sudhir, Liang, T. Jake, ...
Human antibodies elicited in response to hepatitis C virus (HCV) infection are anticipated to react with the native conformation of the viral envelope structure. Isolation of these antibodies as...
Jeong, Sook-Hyang, Qiao, Ming, Nascimbeni, Michelina, Hu, Zongyi, Rehermann, Barbara, Murthy, Krishna, ...
We have previously reported the production of hepatitis C virus-like particles (HCV-LP) using a recombinant baculovirus containing the cDNA of the HCV structural proteins (core, E1, and E2). These...
Steinmann, Daniel, Barth, Heidi, Gissler, Bettina, Schürmann, Peter, Adah, Mohammed I., Gerlach, J. Tilman, ...
Hepatitis C virus (HCV) is a leading cause of chronic viral hepatitis worldwide. The study of antibody-mediated virus neutralization has been hampered by the lack of an efficient and high-throughput...
An in vitro model of hepatitis C virion production
Heller, Theo, Saito, Satoru, Auerbach, Jonathan, Williams, Tarice, Moreen, Tzivia Rachel, Jazwinski, Allison, ...
The hepatitis C virus (HCV) is a major cause of liver disease worldwide. The understanding of the viral life cycle has been hampered by the lack of a satisfactory cell culture system. The development...
Radaeva, Svetlana, Jaruga, Barbara, Kim, Won-Ho, Heller, Theo, Liang, T Jake, Gao, Bin
IFN-gamma (interferon-gamma) modulates IFN-alpha therapy in chronic hepatitis C infection; however, the underlying mechanism remains unclear. Here we demonstrate that long-term (3-6 days) but not...
Cai, Zhaohui, Zhang, Chen, Chang, Kyung-Soo, Jiang, Jieyun, Ahn, Byung-Chul, Wakita, Takaji, ...
Hepatitis C virus (HCV) chronically infects approximately 170 million people worldwide, with an increased risk of developing cirrhosis and hepatocellular carcinoma. The study of HCV replication and...
Altered Proteolysis and Global Gene Expression in Hepatitis B Virus X Transgenic Mouse Liver
Hu, Zongyi, Zhang, Zhensheng, Kim, Jin Woo, Huang, Ying, Liang, T. Jake
Hepatitis B virus X (HBX) is essential for the productive infection of hepatitis B virus (HBV) in vivo and has a pleiotropic effect on host cells. We have previously demonstrated that the proteasome...
Huang, Ying, Yang, Huiying, Borg, Brian B., Su, Xiaowen, Rhodes, Shannon L., Yang, Kai, ...
Cytokine polymorphisms are associated with disease outcome and interferon (IFN) treatment response in hepatitis C virus (HCV) infection. We genotyped eight SNPs spanning the entire IFN-γ gene in two...
Production of Infectious Hepatitis C Virus of Various Genotypes in Cell Cultures▿
Kato, Takanobu, Matsumura, Takuya, Heller, Theo, Saito, Satoru, Sapp, Ronda K., Murthy, Krishna, ...
A unique hepatitis C virus (HCV) strain JFH-1 has been shown to replicate efficiently in cell culture with production of infectious HCV. We previously developed a DNA expression system containing HCV...
Hoofnagle, Jay H., Doo, Edward, Liang, T. Jake, Fleischer, Russell, Lok, Anna S.F.
Chronic hepatitis B is caused by persistent infection with the hepatitis B virus (HBV), a unique DNA virus that replicates through an RNA intermediate produced from a stable covalently closed...
Yee, Leland J., Tang, Yong-Ming, Kleiner, David E., Wang, Dai, Im, KyungAh, Wahed, Abdus, ...
Candidate genes, including myxovirus resistance-1 (Mx1), protein kinase (PKR), transforming growth factor- Β 1 (TGF-Β), interleukin-10 (IL-10), and interferon-gamma (IFN-Γ), were evaluated for...
Elmowalid, Gamal A., Qiao, Ming, Jeong, Sook-Hyang, Borg, Brian B., Baumert, Thomas F., Sapp, Ronda K., ...
Recombinant hepatitis C virus (HCV)-like particles (HCV-LPs) containing HCV structural proteins (core, E1, and E2) produced in insect cells resemble the putative HCV virions and are capable of...
Barth, Heidi, Schnober, Eva K., Neumann-Haefelin, Christoph, Thumann, Christine, Zeisel, Mirjam B., Diepolder, Helmut M., ...
Class B scavenger receptors (SR-Bs) bind lipoproteins and play an important role in lipid metabolism. Most recently, SR-B type I (SR-BI) and its splicing variant SR-BII have been found to mediate...
Nanda, Santosh, Havert, Michael B., Calderón, Gloria M., Thomson, Michael, Jacobson, Christian, Kastner, Daniel, ...
Hepatitis C virus infection leads to a high rate of chronicity. Mechanisms of viral clearance and persistence are still poorly understood. In this study, hepatic gene expression analysis was...
Yee, Leland J., Im, KyungAh, Wahed, Abdus S., Bugawan, Teodorica, Li, Jia, Rhodes, Shannon L., ...
The dynamics of the viral decline immediately after the start of therapy for chronic hepatitis C virus (HCV) infection may have prognostic potential for ultimate sustained virologic response....