T. John Andrews

Regulation of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase by Carbamylation and 2-Carboxyarabinitol 1-Phosphate in Tobacco: Insights from Studies of Antisense Plants Containing Reduced Amounts of Rubisco Activase1

Hammond, Edward T., Andrews, T. John, Woodrow, Ian E.

The regulation of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activity by 2-carboxyarabinitol 1-phosphate (CA1P) was investigated using gas-exchange analysis of antisense tobacco...

The Role of Chloroplast Electron Transport and Metabolites in Modulating Rubisco Activity in Tobacco. Insights from Transgenic Plants with Reduced Amounts of Cytochrome b/f Complex or Glyceraldehyde 3-Phosphate Dehydrogenase1

Ruuska, Sari A., Andrews, T. John, Badger, Murray R., Price, G. Dean, Von Caemmerer, Susanne

Leaf metabolites, adenylates, and Rubisco activation were studied in two transgenic tobacco (Nicotiana tabacum L. cv W38) types. Plants with reduced amounts of cytochrome b/f complex (anti-b/f) have...

Directed Mutation of the Rubisco Large Subunit of Tobacco Influences Photorespiration and Growth1

Whitney, Spencer M., Von Caemmerer, Susanne, Hudson, Graham S., Andrews, T. John

The gene for the large subunit of Rubisco was specifically mutated by transforming the chloroplast genome of tobacco (Nicotiana tabacum). Codon 335 was altered to encode valine instead of leucine....

Plastome-encoded bacterial ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) supports photosynthesis and growth in tobacco

Whitney, Spencer M., Andrews, T. John

The efficiency with which crop plants use their resources of light, water, and fertilizer nitrogen could be enhanced by replacing their CO2-fixing enzyme, d-ribulose-1,5-bisphosphate...

The Gene for the Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase (Rubisco) Small Subunit Relocated to the Plastid Genome of Tobacco Directs the Synthesis of Small Subunits That Assemble into Rubisco

Whitney, Spencer M., Andrews, T. John

To assess the extent to which a nuclear gene for a chloroplast protein retained the ability to be expressed in its presumed preendosymbiotic location, we relocated the RbcS gene for the small subunit...

Photosynthesis and Growth of Tobacco with a Substituted Bacterial Rubisco Mirror the Properties of the Introduced Enzyme

Whitney, Spencer M., Andrews, T. John

Complete replacement, by biolistic plastid transformation, of the hexadecameric ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) of tobacco (Nicotiana tabacum) with the dimeric version from...

Nitrogen-Regulated Hypermutator Strain of Synechococcus sp. for Use in In Vivo Artificial Evolution

Emlyn-Jones, Daniel, Price, G. Dean, Andrews, T. John

Artificially evolved variants of proteins with roles in photosynthesis may be selected most conveniently by using a photosynthetic organism, such as a cyanobacterium, whose growth depends on the...

Active-site carbamate formation and reaction-intermediate-analog binding by ribulosebisphosphate carboxylase/oxygenase in the absence of its small subunits

Andrews, T. John, Ballment, Beth

Even though depleted of more than 90% of its small subunits, ribulose 1,5-bisphosphate carboxylase/oxygenase from Synechococcus ACMM 323 still formed a stable complex with 2-carboxyarabinitol...

Effects of pH and Oxygen on Photosynthetic Reactions of Intact Chloroplasts 1

Heber, Ulrich, Andrews, T. John, Boardman, N. Keith

Oxygen inhibition of photosynthesis was studied with intact spinach (Spinacia oleracea L.) chloroplasts which exhibited very high rates of photosynthetic CO2 reduction and were insensitive to...

Photosynthetic Carbon Metabolism in Seagrasses 14C-Labeling Evidence for the C3 Pathway

Andrews, T. John, Abel, Kay M.

The δ13C values of several seagrasses were considerably less negative than those of terrestrial C3 plants and tended toward those of terrestrial C4 plants. However, for Thalassia hemprichii...

A Kinetic Characterization of Slow Inactivation of Ribulosebisphosphate Carboxylase during Catalysis

Edmondson, Daryl L., Badger, Murray R., Andrews, T. John

The catalytic activity of ribulosebisphosphate carboxylase (Rubisco) declined as soon as catalysis was initiated by exposure to its substrate, d-ribulose-1,5-bisphosphate (ribulose-P2). The decline...

Slow Inactivation of Ribulosebisphosphate Carboxylase during Catalysis Is Not Due to Decarbamylation of the Catalytic Site

Edmondson, Daryl L., Badger, Murray R., Andrews, T. John

An investigation was made of the proposal that the slow inactivation of ribulosebisphosphate carboxylase (Rubisco) activity, which occurs during in vitro assays, is due to decarbamylation of the...

Slow Inactivation of Ribulosebisphosphate Carboxylase during Catalysis Is Caused by Accumulation of a Slow, Tight-Binding Inhibitor at the Catalytic Site

Edmondson, Daryl L., Badger, Murray R., Andrews, T. John

The slow inactivation which accompanies catalysis by higher-plant ribulose-P2 carboxylase-oxygenase (Rubisco) in vitro was only partially reversed when the enzyme was gel filtered to remove small...

Faster Rubisco Is the Key to Superior Nitrogen-Use Efficiency in NADP-Malic Enzyme Relative to NAD-Malic Enzyme C4 Grasses1

Ghannoum, Oula, Evans, John R., Chow, Wah Soon, Andrews, T. John, Conroy, Jann P., Von Caemmerer, Susanne

In 27 C4 grasses grown under adequate or deficient nitrogen (N) supplies, N-use efficiency at the photosynthetic (assimilation rate per unit leaf N) and whole-plant (dry mass per total leaf N) level...

A Rapid, Sensitive Method for Quantitating Subunits in Purified Ribulose Bisphosphate Carboxylase Preparations 1

Andrews, T. John, Ballment, Beth

Studies of the interactions of the large and small subunits of ribulose bisphosphate carboxylase require a knowledge of the concentrations of the subunits present in various preparations and their...

Photosynthesis and Inorganic Carbon Usage by the Marine Cyanobacterium, Synechococcus sp

Badger, Murray R., Andrews, T. John

The marine cyanobacterium, Synechococcus sp. Nägeli (strain RRIMP N1) changes its affinity for external inorganic carbon used in photosynthesis, depending on the concentration of CO2 provided during...

Reduction of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Content by Antisense RNA Reduces Photosynthesis in Transgenic Tobacco Plants 1

Hudson, Graham S., Evans, John R., Von Caemmerer, Susanne, Arvidsson, Yvonne B. C., Andrews, T. John

A complementary DNA for the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) was cloned from tobacco (Nicotiana tabacum) and fused in the antisense orientation to the...

Despite slow catalysis and confused substrate specificity, all ribulose bisphosphate carboxylases may be nearly perfectly optimized

Tcherkez, Guillaume G. B., Farquhar, Graham D., Andrews, T. John

The cornerstone of autotrophy, the CO2-fixing enzyme, d-ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), is hamstrung by slow catalysis and confusion between CO2 and O2 as substrates, an...

Regulation of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase by Carbamylation and 2-Carboxyarabinitol 1-Phosphate in Tobacco: Insights from Studies of Antisense Plants Containing Reduced Amounts of Rubisco Activase1

Hammond, Edward T., Andrews, T. John, Woodrow, Ian E.

The regulation of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activity by 2-carboxyarabinitol 1-phosphate (CA1P) was investigated using gas-exchange analysis of antisense tobacco...

The Role of Chloroplast Electron Transport and Metabolites in Modulating Rubisco Activity in Tobacco. Insights from Transgenic Plants with Reduced Amounts of Cytochrome b/f Complex or Glyceraldehyde 3-Phosphate Dehydrogenase1

Ruuska, Sari A., Andrews, T. John, Badger, Murray R., Price, G. Dean, Von Caemmerer, Susanne

Leaf metabolites, adenylates, and Rubisco activation were studied in two transgenic tobacco (Nicotiana tabacum L. cv W38) types. Plants with reduced amounts of cytochrome b/f complex (anti-b/f) have...

Directed Mutation of the Rubisco Large Subunit of Tobacco Influences Photorespiration and Growth1

Whitney, Spencer M., Von Caemmerer, Susanne, Hudson, Graham S., Andrews, T. John

The gene for the large subunit of Rubisco was specifically mutated by transforming the chloroplast genome of tobacco (Nicotiana tabacum). Codon 335 was altered to encode valine instead of leucine....

Plastome-encoded bacterial ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) supports photosynthesis and growth in tobacco

Whitney, Spencer M., Andrews, T. John

The efficiency with which crop plants use their resources of light, water, and fertilizer nitrogen could be enhanced by replacing their CO2-fixing enzyme, d-ribulose-1,5-bisphosphate...

The Gene for the Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase (Rubisco) Small Subunit Relocated to the Plastid Genome of Tobacco Directs the Synthesis of Small Subunits That Assemble into Rubisco

Whitney, Spencer M., Andrews, T. John

To assess the extent to which a nuclear gene for a chloroplast protein retained the ability to be expressed in its presumed preendosymbiotic location, we relocated the RbcS gene for the small subunit...

Photosynthesis and Growth of Tobacco with a Substituted Bacterial Rubisco Mirror the Properties of the Introduced Enzyme

Whitney, Spencer M., Andrews, T. John

Complete replacement, by biolistic plastid transformation, of the hexadecameric ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) of tobacco (Nicotiana tabacum) with the dimeric version from...

Nitrogen-Regulated Hypermutator Strain of Synechococcus sp. for Use in In Vivo Artificial Evolution

Emlyn-Jones, Daniel, Price, G. Dean, Andrews, T. John

Artificially evolved variants of proteins with roles in photosynthesis may be selected most conveniently by using a photosynthetic organism, such as a cyanobacterium, whose growth depends on the...

Active-site carbamate formation and reaction-intermediate-analog binding by ribulosebisphosphate carboxylase/oxygenase in the absence of its small subunits

Andrews, T. John, Ballment, Beth

Even though depleted of more than 90% of its small subunits, ribulose 1,5-bisphosphate carboxylase/oxygenase from Synechococcus ACMM 323 still formed a stable complex with 2-carboxyarabinitol...

Effects of pH and Oxygen on Photosynthetic Reactions of Intact Chloroplasts 1

Heber, Ulrich, Andrews, T. John, Boardman, N. Keith

Oxygen inhibition of photosynthesis was studied with intact spinach (Spinacia oleracea L.) chloroplasts which exhibited very high rates of photosynthetic CO2 reduction and were insensitive to...

Photosynthetic Carbon Metabolism in Seagrasses 14C-Labeling Evidence for the C3 Pathway

Andrews, T. John, Abel, Kay M.

The δ13C values of several seagrasses were considerably less negative than those of terrestrial C3 plants and tended toward those of terrestrial C4 plants. However, for Thalassia hemprichii...

A Kinetic Characterization of Slow Inactivation of Ribulosebisphosphate Carboxylase during Catalysis

Edmondson, Daryl L., Badger, Murray R., Andrews, T. John

The catalytic activity of ribulosebisphosphate carboxylase (Rubisco) declined as soon as catalysis was initiated by exposure to its substrate, d-ribulose-1,5-bisphosphate (ribulose-P2). The decline...

Slow Inactivation of Ribulosebisphosphate Carboxylase during Catalysis Is Not Due to Decarbamylation of the Catalytic Site

Edmondson, Daryl L., Badger, Murray R., Andrews, T. John

An investigation was made of the proposal that the slow inactivation of ribulosebisphosphate carboxylase (Rubisco) activity, which occurs during in vitro assays, is due to decarbamylation of the...

Slow Inactivation of Ribulosebisphosphate Carboxylase during Catalysis Is Caused by Accumulation of a Slow, Tight-Binding Inhibitor at the Catalytic Site

Edmondson, Daryl L., Badger, Murray R., Andrews, T. John

The slow inactivation which accompanies catalysis by higher-plant ribulose-P2 carboxylase-oxygenase (Rubisco) in vitro was only partially reversed when the enzyme was gel filtered to remove small...

Faster Rubisco Is the Key to Superior Nitrogen-Use Efficiency in NADP-Malic Enzyme Relative to NAD-Malic Enzyme C4 Grasses1

Ghannoum, Oula, Evans, John R., Chow, Wah Soon, Andrews, T. John, Conroy, Jann P., Von Caemmerer, Susanne

In 27 C4 grasses grown under adequate or deficient nitrogen (N) supplies, N-use efficiency at the photosynthetic (assimilation rate per unit leaf N) and whole-plant (dry mass per total leaf N) level...

A Rapid, Sensitive Method for Quantitating Subunits in Purified Ribulose Bisphosphate Carboxylase Preparations 1

Andrews, T. John, Ballment, Beth

Studies of the interactions of the large and small subunits of ribulose bisphosphate carboxylase require a knowledge of the concentrations of the subunits present in various preparations and their...

Photosynthesis and Inorganic Carbon Usage by the Marine Cyanobacterium, Synechococcus sp

Badger, Murray R., Andrews, T. John

The marine cyanobacterium, Synechococcus sp. Nägeli (strain RRIMP N1) changes its affinity for external inorganic carbon used in photosynthesis, depending on the concentration of CO2 provided during...

Reduction of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Content by Antisense RNA Reduces Photosynthesis in Transgenic Tobacco Plants 1

Hudson, Graham S., Evans, John R., Von Caemmerer, Susanne, Arvidsson, Yvonne B. C., Andrews, T. John

A complementary DNA for the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) was cloned from tobacco (Nicotiana tabacum) and fused in the antisense orientation to the...

Despite slow catalysis and confused substrate specificity, all ribulose bisphosphate carboxylases may be nearly perfectly optimized

Tcherkez, Guillaume G. B., Farquhar, Graham D., Andrews, T. John

The cornerstone of autotrophy, the CO2-fixing enzyme, d-ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), is hamstrung by slow catalysis and confusion between CO2 and O2 as substrates, an...