Iwasawa, T, Komano, T, Tajima, A, Tokunaga, M, Obora, Y, Fujihara, T, ...
Yoshida, S, Suzuki, M, Yamano, S, Takeuchi, M, Ikenaga, H, Kioka, N, ...
Honda, Y, Sakai, H, Hiasa, H, Tanaka, K, Komano, T, Bagdasarian, M
Two single-stranded DNA initiation signals (designated ssi) present in the origin of vegetative DNA replication (oriV) of the broad-host-range plasmid RSF1010 are essential for the priming of...
Products of the nikA and nikB genes of plasmid R64 have been shown to form a relaxation complex with R64 oriT DNA and to function together as an oriT-specific nickase. We purified the protein product...
A 6.72-kb DNA sequence between the exc gene and the oriT operon within the transfer region of IncI1 plasmid R64 was sequenced and characterized. Three novel transfer genes, trbA, trbB, and trbC, were...
Tojo, N, Sanmiya, K, Sugawara, H, Inouye, S, Komano, T
Mx8 is a generalized transducing phage that infects Myxococcus xanthus cells. This phage is lysogenized in M. xanthus cells by the integration of its DNA into the host chromosome through...
Analysis of DNA inversions in the shufflon of plasmid R64.
Gyohda, A, Funayama, N, Komano, T
The shufflon, a multiple DNA inversion system in the plasmid R64, consists of four DNA segments flanked and separated by seven 19-bp repeat sequences. Site-specific recombinations mediated by the rci...
The plasmid R64 thin pilus identified as a type IV pilus.
The entire nucleotide sequence of the pil region of the IncI1 plasmid R64 was determined. Analysis of the sequence indicated that 14 genes, designated pilI through pilV, are involved in the formation...
Functional features of an ssi signal of plasmid pGKV21 in Escherichia coli.
Jeong, J Y, Yoon, G M, Seo, H S, Cho, M J, Sakai, H, Komano, T, ...
A single-strand initiation (ssi) signal was detected on the Lactococcus lactis plasmid pGKV21 containing the replicon of pWV01 by its ability to complement the poor growth of an M13 phage derivative...
Conjugative DNA transfer of IncI1 plasmid R64 is initiated by the introduction of a site- and strand-specific nick into the origin of transfer (oriT). In R64 oriT, 17-bp (repeat A and B) and 8-bp...
The lon gene of Escherichia coli is known to encode protease La, an ATP-dependent protease associated with cellular protein degradation. A lon gene homolog from Myxococcus xanthus, a soil bacterium...
Yoshisue, H, Fukada, T, Yoshida, K, Sen, K, Kurosawa, S, Sakai, H, ...
The cryIVA gene encodes a component of the delta-endotoxin of Bacillus thuringiensis subsp. israelensis. By S1 nuclease mapping and primer extension analysis, we have identified the transcriptional...
Myxococcus xanthus contains two genes (lonV and lonD) homologous to the Escherichia coli lon gene for an ATP-dependent protease. We found that the lonD gene encodes a 90-kDa protein consisting of 827...
Nucleotide sequence and characterization of the traABCD region of IncI1 plasmid R64.
Kim, S R, Funayama, N, Komano, T
A 3.6-kb BglII-SmaI segment of the transfer region of IncI1 plasmid R64drd-11 was sequenced and characterized. Analysis of the DNA sequence indicated the presence of four genes, traA, traB, traC, and...
Tanaka, K, Rogi, T, Hiasa, H, Miao, D M, Honda, Y, Nomura, N, ...
The primase-dependent priming signals, G sites, are directly recognized by the Escherichia coli primase (dnaG gene product) and conduct the synthesis of primer RNAs. In nucleotide sequence and...
Nucleotide sequence of the R721 shufflon.
The shufflon is a DNA region that undergoes complex rearrangement mediated by the product of a putative site-specific recombinase gene, rci. The DNA sequences of the shufflon region and the rci gene...
A unique repetitive DNA sequence in the Myxococcus xanthus genome.
Fujitani, S, Komano, T, Inouye, S
We found a novel type of repetitive DNA sequence in the Myxococcus xanthus genome. The first repetitive sequence is located in the spacer region between the ops and tps genes. We cloned five other...
Nucleotide sequence and functions of the oriT operon in IncI1 plasmid R64.
Furuya, N, Nisioka, T, Komano, T
Two transfer genes of IncI1 plasmid R64, tentatively designated nikA and nikB, were cloned and sequenced. They are located adjacent to the origin of transfer (oriT) and appear to be organized into an...
Kawamukai, M, Utsumi, R, Takeda, K, Higashi, A, Matsuda, H, Choi, Y L, ...
We have cloned at least 12 different Escherichia coli genes which enable strain MK2001 to use maltose. The genes were designated sfs1 through sfs12 (sugar fermentation stimulation). Previously, one...
Transfer region of IncI1 plasmid R64 and role of shufflon in R64 transfer.
Komano, T, Funayama, N, Kim, S R, Nisioka, T
To locate the transfer region of the 122-kiloase plasmid R64drd-11 belonging to incompatibility group I1, a series of deletion derivatives was constructed by in vitro recombinant DNA techniques...
The nick site at the origin of transfer, oriT, of IncI1 plasmid R64 was determined. A site-specific and strand-specific cleavage of the phosphodiester bond was introduced during relaxation of the...
Utsumi, R, Noda, M, Kawamukai, M, Komano, T
The role of cyclic AMP (cAMP) in the cell cycle of Escherichia coli K-12 was studied in three mutant strains. One was KI1812, in which the cya promoter is replaced by the lacUV5 promoter. In KI1812,...
Kawamukai, M, Matsuda, H, Fujii, W, Utsumi, R, Komano, T
The nucleotide sequences of fic-1 involved in the cell filamentation induced by cyclic AMP in Escherichia coli and its normal counterpart fic were analyzed. The open reading frame of both fic-1 and...
Choi, Y L, Nishida, T, Kawamukai, M, Utsumi, R, Sakai, H, Komano, T
An nlp (Ner-like protein) gene was isolated from Escherichia coli. The nucleotide sequence of a 1,342-base-pair chromosomal DNA fragment containing the nlp gene was analyzed. It contained two open...
Kawamukai, M, Matsuda, H, Fujii, W, Nishida, T, Izumoto, Y, Himeno, M, ...
PA3092 is an Escherichia coli mutant that forms filaments at 43 degrees C in the presence of cyclic AMP (cAMP). The mutation responsible for this phenotype is called fic-1. We cloned fic-1 from...
Cloning and nucleotide sequence of the oriT region of the IncI1 plasmid R64.
Komano, T, Toyoshima, A, Morita, K, Nisioka, T
The nucleotide sequence at the oriT region of the IncI1 plasmid R64 was determined. A recombinant plasmid carrying a 141-base-pair R64 sequence was mobilized with a normal frequency, while a plasmid...
Expression of the adenylate cyclase gene during cell elongation in Escherichia coli K-12.
Utsumi, R, Kawamukai, M, Aiba, H, Himeno, M, Komano, T
Expression of the adenylate cyclase gene (cya) in synchronized Escherichia coli cells was investigated by using the cya-lacZ protein and operon fusion plasmids. The regulation of cya expression...
Distribution of shufflon among IncI plasmids.
Komano, T, Kim, S R, Nisioka, T
A shufflon or clustered inversion is a novel type of DNA rearrangement originally discovered in the IncI1 plasmid R64 (T. Komano, A. Kubo, and T. Nisioka, Nucleic Acids Res. 15:1165-1172, 1987). In a...
Kawamukai, M, Kishimoto, J, Utsumi, R, Himeno, M, Komano, T, Aiba, H
We constructed cya-lac protein and operon fusion plasmids in vitro. The effect of cyclic AMP (cAMP) on cya expression was examined by measuring the synthesis of beta-galactosidase in Escherichia coli...
Highly mobile DNA segment of IncI alpha plasmid R64: a clustered inversion region.
Komano, T, Kubo, A, Kayanuma, T, Furuichi, T, Nisioka, T
When R64 DNA was digested with EcoRI, two DNA fragments not equimolar to the plasmid DNA were produced. A DNA region including these fragments was cloned (pKK009), and the pKK009 DNA sample was found...
Physical and genetic analyses of the Inc-I alpha plasmid R64.
Furuichi, T, Komano, T, Nisioka, T
A 126-kilobase (kb) physical and genetic map of the Inc-I alpha plasmid R64 was constructed by using the restriction enzymes, BamHI, SalI, XhoI, HindIII, and EcoRI. The replication (Rep) and...
Komano, T, Furuichi, T, Teintze, M, Inouye, M, Inouye, S
A deletion mutation of the gene for protein S (tps), a development-specific protein of Myxococcus xanthus, was constructed. No significant differences in the process of fruiting body formation or the...
Utsumi, R, Tanabe, H, Nakamoto, Y, Kawamukai, M, Sakai, H, Himeno, M, ...
Cell division of Escherichia coli K-12 strain PA3092 was inhibited by the addition of adenosine 3',5'-phosphate (cAMP), and the cellular morphology was changed from rods into filaments. Nucleoids in...
Miyakawa, Y, Komano, T, Maruyama, Y
Four isogenic strains of temperature-sensitive septationless mutants, whose mutations are located on different genes, were used to study the periods of action of the gene products required for the...
Komano, T, Inouye, S, Inouye, M
Myxococcus xanthus was pulse-labeled with [3H]thymidine immediately after germination of dimethyl sulfoxide-induced spores. The restriction enzyme digests of the total chromosomal DNA from the...
Phosphorylation and methylation of proteins during Myxococcus xanthus spore formation.
Komano, T, Brown, N, Inouye, S, Inouye, M
Post-translational modification of proteins was examined during the life cycle of Myxococcus xanthus. A specific pattern of protein phosphorylation was observed in vegetative cells. When spore...
Utsumi, R, Nakamoto, Y, Kawamukai, M, Himeno, M, Komano, T
Cyclic AMP (cAMP) inhibited septum formation in Escherichia coli PA3092 and induced cell filamentation at elevated temperatures. This phenomenon was first observed in E. coli PA3092 and is due to a...
Honjo, M, Shibano, Y, Komano, T
The deoxyribonucleic acid (DNA) polymerase activities in Bacillus subtilis strains Marburg 168 (thy-trp2) and D22, a DNA polymerase I-deficient mutant, were measured at various stages of sporulation....
Miyakawa, Y, Komano, T, Maruyama, Y
The relationship between protein synthesis and processes of cell division was studied by using synchronized cells of Bacillus subtilis 168. The addition of chloramphenicol at the beginning of...
Miao, D M, Sakai, H, Okamoto, S, Tanaka, K, Okuda, M, Honda, Y, ...
The replication origin of the broad host-range plasmid RSF1010 contains 3.5 copies of a 20mer iteron sequence that bind specifically to the plasmid-encoded initiator, RepC. Here we demonstrated that...
Tanaka, K, Kino, K, Taguchi, Y, Miao, D M, Honda, Y, Sakai, H, ...
The broad host-range plasmid RSF1010 contains two oppositely oriented priming signals, ssiA and ssiB, for DNA synthesis dependent on the origin of vegetative DNA replication (oriV). If either ssiA or...
Miao, D M, Honda, Y, Tanaka, K, Higashi, A, Nakamura, T, Taguchi, Y, ...
The two single-strand DNA initiation signals, ssiA(RSF1010) and ssiB(RSF1010) of the broad host-range plasmid RSF1010 contain proposed stem-loop structures. Nine single base-change mutations in the...
Honda, Y, Nakamura, T, Tanaka, K, Higashi, A, Sakai, H, Komano, T, ...
Broad host-range plasmid RSF1010 contains in the oriV region two DNA initiation signals, ssiA(RSF1010) and ssiB(RSF1010), which are essential for plasmid replication. Each of ssiA and ssiB could be...
Saito, I, Sugiyama, H, Matsuura, T, Ueda, K, Komano, T
A new procedure for T specific cleavage of DNA fragments utilizing photoreaction with spermine has been described. Irradiation of 3'-[32P]-end-labeled DNA fragments for 10-20 min with a germicidal...
Yamano, Y, Ohyama, K, Komano, T
The nucleotide sequences of chloroplast 5S rRNAs from cell suspension cultures of the liverworts Marchantia polymorpha and Jungermannia subulata were determined. Their nucleotide sequences, 119...
Sequence-specific DNA damage induced by reduced mitomycin C and 7-N-(p-hydroxyphenyl)mitomycin C.
Mitomycin C reduced with sodium borohydride induced the DNA damage at deoxyguanosines preferentially in dinucleotide sequence G-T. The DNA damage produced strand breaks when subsequently heated. The...
Hiasa, H, Sakai, H, Komano, T, Godson, G N
45 mutations (insertion, deletion and base substitution) of the G4 Goric were tested for their functional activity in M13 and R199 in vivo. The critical mutants were also assayed for their ability to...
Nucleotide sequence of the growth hormone gene cDNA from goat Capra hircus L. (Tokara).
Yato, M, Yamano, Y, Oyabayashi, K, Okuno, M, Kioka, N, Manabe, E, ...
Tanaka, K, Sakai, H, Honda, Y, Nakamura, T, Higashi, A, Komano, T
We have structurally and functionally analyzed the cis-elements essential for ColIb-P9 plasmid DNA replication. The putative oriV region encompassed a region of 172 base pairs (bp) located 152 bp...
Choi, Y L, Kawase, S, Nishida, T, Sakai, H, Komano, T, Kawamukai, M, ...
A nicked group II intron and trans-splicing in liverwort, Marchantia polymorpha, chloroplasts.
Kohchi, T, Umesono, K, Ogura, Y, Komine, Y, Nakahigashi, K, Komano, T, ...
The chloroplast gene rps12 for ribosomal protein S12 in a liverwort, Marchantia polymorpha, is split into three exons by two introns, one of which (intron 1) is discontinuous. Exon 1 of rps12 for the...
Komano, T, Kubo, A, Nisioka, T
The IncI alpha plasmid R64 was found to bear a highly mobile DNA segment which was designated as a clustered inversion region (J. Bacteriol. 165, 94-100, 1986). The clustered inversion region...
Sequence specific damage of DNA induced by reducing sugars.
Morita, J, Ueda, K, Nanjo, S, Komano, T
Reducing sugars induced alkali-labile sites in DNA. The DNA reacted with D-fructose 6-phosphate or D-fructose in the presence of Cu2+ was cleaved by the treatment with aqueous piperidine at 90...
Growth and DNA synthesis of bacteriophage phi x174 in a dnaP mutant of Escherichia coli.
phi X174am3trD, a temperature-resistant mutant of bacteriophage phi X174am3, exhibited a reduced ability to grow in a dnaP mutant, Escherichia coli KM107, at the restrictive temperature (43 degrees...
Divergent mRNA transcription in the chloroplast psbB operon.
Kohchi, T, Yoshida, T, Komano, T, Ohyama, K
The genes psbB, psbH, petB and petD for the components in photosystem II and the cytochrome b6/f complex are clustered and co-transcribed in liverwort Marchantia polymorpha chloroplasts. On the...
Utsumi, R, Kawamukai, M, Obata, K, Morita, J, Himeno, M, Komano, T
A membrane protein with a molecular weight of 40,000 (40K protein) was induced concurrently with cell filamentation by cyclic AMP (cAMP) in a fic mutant. In the crp mutant and the wild-type strain,...
Honda, Y, Sakai, H, Hiasa, H, Tanaka, K, Komano, T, Bagdasarian, M
Two single-stranded DNA initiation signals (designated ssi) present in the origin of vegetative DNA replication (oriV) of the broad-host-range plasmid RSF1010 are essential for the priming of...
Products of the nikA and nikB genes of plasmid R64 have been shown to form a relaxation complex with R64 oriT DNA and to function together as an oriT-specific nickase. We purified the protein product...
A 6.72-kb DNA sequence between the exc gene and the oriT operon within the transfer region of IncI1 plasmid R64 was sequenced and characterized. Three novel transfer genes, trbA, trbB, and trbC, were...
Tojo, N, Sanmiya, K, Sugawara, H, Inouye, S, Komano, T
Mx8 is a generalized transducing phage that infects Myxococcus xanthus cells. This phage is lysogenized in M. xanthus cells by the integration of its DNA into the host chromosome through...
Analysis of DNA inversions in the shufflon of plasmid R64.
Gyohda, A, Funayama, N, Komano, T
The shufflon, a multiple DNA inversion system in the plasmid R64, consists of four DNA segments flanked and separated by seven 19-bp repeat sequences. Site-specific recombinations mediated by the rci...
The plasmid R64 thin pilus identified as a type IV pilus.
The entire nucleotide sequence of the pil region of the IncI1 plasmid R64 was determined. Analysis of the sequence indicated that 14 genes, designated pilI through pilV, are involved in the formation...
Functional features of an ssi signal of plasmid pGKV21 in Escherichia coli.
Jeong, J Y, Yoon, G M, Seo, H S, Cho, M J, Sakai, H, Komano, T, ...
A single-strand initiation (ssi) signal was detected on the Lactococcus lactis plasmid pGKV21 containing the replicon of pWV01 by its ability to complement the poor growth of an M13 phage derivative...
Conjugative DNA transfer of IncI1 plasmid R64 is initiated by the introduction of a site- and strand-specific nick into the origin of transfer (oriT). In R64 oriT, 17-bp (repeat A and B) and 8-bp...
The lon gene of Escherichia coli is known to encode protease La, an ATP-dependent protease associated with cellular protein degradation. A lon gene homolog from Myxococcus xanthus, a soil bacterium...
Yoshisue, H, Fukada, T, Yoshida, K, Sen, K, Kurosawa, S, Sakai, H, ...
The cryIVA gene encodes a component of the delta-endotoxin of Bacillus thuringiensis subsp. israelensis. By S1 nuclease mapping and primer extension analysis, we have identified the transcriptional...
Myxococcus xanthus contains two genes (lonV and lonD) homologous to the Escherichia coli lon gene for an ATP-dependent protease. We found that the lonD gene encodes a 90-kDa protein consisting of 827...
Nucleotide sequence and characterization of the traABCD region of IncI1 plasmid R64.
Kim, S R, Funayama, N, Komano, T
A 3.6-kb BglII-SmaI segment of the transfer region of IncI1 plasmid R64drd-11 was sequenced and characterized. Analysis of the DNA sequence indicated the presence of four genes, traA, traB, traC, and...
Tanaka, K, Rogi, T, Hiasa, H, Miao, D M, Honda, Y, Nomura, N, ...
The primase-dependent priming signals, G sites, are directly recognized by the Escherichia coli primase (dnaG gene product) and conduct the synthesis of primer RNAs. In nucleotide sequence and...
Nucleotide sequence of the R721 shufflon.
The shufflon is a DNA region that undergoes complex rearrangement mediated by the product of a putative site-specific recombinase gene, rci. The DNA sequences of the shufflon region and the rci gene...
A unique repetitive DNA sequence in the Myxococcus xanthus genome.
Fujitani, S, Komano, T, Inouye, S
We found a novel type of repetitive DNA sequence in the Myxococcus xanthus genome. The first repetitive sequence is located in the spacer region between the ops and tps genes. We cloned five other...
Nucleotide sequence and functions of the oriT operon in IncI1 plasmid R64.
Furuya, N, Nisioka, T, Komano, T
Two transfer genes of IncI1 plasmid R64, tentatively designated nikA and nikB, were cloned and sequenced. They are located adjacent to the origin of transfer (oriT) and appear to be organized into an...
Kawamukai, M, Utsumi, R, Takeda, K, Higashi, A, Matsuda, H, Choi, Y L, ...
We have cloned at least 12 different Escherichia coli genes which enable strain MK2001 to use maltose. The genes were designated sfs1 through sfs12 (sugar fermentation stimulation). Previously, one...
Transfer region of IncI1 plasmid R64 and role of shufflon in R64 transfer.
Komano, T, Funayama, N, Kim, S R, Nisioka, T
To locate the transfer region of the 122-kiloase plasmid R64drd-11 belonging to incompatibility group I1, a series of deletion derivatives was constructed by in vitro recombinant DNA techniques...
The nick site at the origin of transfer, oriT, of IncI1 plasmid R64 was determined. A site-specific and strand-specific cleavage of the phosphodiester bond was introduced during relaxation of the...
Utsumi, R, Noda, M, Kawamukai, M, Komano, T
The role of cyclic AMP (cAMP) in the cell cycle of Escherichia coli K-12 was studied in three mutant strains. One was KI1812, in which the cya promoter is replaced by the lacUV5 promoter. In KI1812,...
Kawamukai, M, Matsuda, H, Fujii, W, Utsumi, R, Komano, T
The nucleotide sequences of fic-1 involved in the cell filamentation induced by cyclic AMP in Escherichia coli and its normal counterpart fic were analyzed. The open reading frame of both fic-1 and...
Choi, Y L, Nishida, T, Kawamukai, M, Utsumi, R, Sakai, H, Komano, T
An nlp (Ner-like protein) gene was isolated from Escherichia coli. The nucleotide sequence of a 1,342-base-pair chromosomal DNA fragment containing the nlp gene was analyzed. It contained two open...
Kawamukai, M, Matsuda, H, Fujii, W, Nishida, T, Izumoto, Y, Himeno, M, ...
PA3092 is an Escherichia coli mutant that forms filaments at 43 degrees C in the presence of cyclic AMP (cAMP). The mutation responsible for this phenotype is called fic-1. We cloned fic-1 from...
Cloning and nucleotide sequence of the oriT region of the IncI1 plasmid R64.
Komano, T, Toyoshima, A, Morita, K, Nisioka, T
The nucleotide sequence at the oriT region of the IncI1 plasmid R64 was determined. A recombinant plasmid carrying a 141-base-pair R64 sequence was mobilized with a normal frequency, while a plasmid...
Expression of the adenylate cyclase gene during cell elongation in Escherichia coli K-12.
Utsumi, R, Kawamukai, M, Aiba, H, Himeno, M, Komano, T
Expression of the adenylate cyclase gene (cya) in synchronized Escherichia coli cells was investigated by using the cya-lacZ protein and operon fusion plasmids. The regulation of cya expression...
Distribution of shufflon among IncI plasmids.
Komano, T, Kim, S R, Nisioka, T
A shufflon or clustered inversion is a novel type of DNA rearrangement originally discovered in the IncI1 plasmid R64 (T. Komano, A. Kubo, and T. Nisioka, Nucleic Acids Res. 15:1165-1172, 1987). In a...
Kawamukai, M, Kishimoto, J, Utsumi, R, Himeno, M, Komano, T, Aiba, H
We constructed cya-lac protein and operon fusion plasmids in vitro. The effect of cyclic AMP (cAMP) on cya expression was examined by measuring the synthesis of beta-galactosidase in Escherichia coli...
Highly mobile DNA segment of IncI alpha plasmid R64: a clustered inversion region.
Komano, T, Kubo, A, Kayanuma, T, Furuichi, T, Nisioka, T
When R64 DNA was digested with EcoRI, two DNA fragments not equimolar to the plasmid DNA were produced. A DNA region including these fragments was cloned (pKK009), and the pKK009 DNA sample was found...
Physical and genetic analyses of the Inc-I alpha plasmid R64.
Furuichi, T, Komano, T, Nisioka, T
A 126-kilobase (kb) physical and genetic map of the Inc-I alpha plasmid R64 was constructed by using the restriction enzymes, BamHI, SalI, XhoI, HindIII, and EcoRI. The replication (Rep) and...
Komano, T, Furuichi, T, Teintze, M, Inouye, M, Inouye, S
A deletion mutation of the gene for protein S (tps), a development-specific protein of Myxococcus xanthus, was constructed. No significant differences in the process of fruiting body formation or the...
Utsumi, R, Tanabe, H, Nakamoto, Y, Kawamukai, M, Sakai, H, Himeno, M, ...
Cell division of Escherichia coli K-12 strain PA3092 was inhibited by the addition of adenosine 3',5'-phosphate (cAMP), and the cellular morphology was changed from rods into filaments. Nucleoids in...
Miyakawa, Y, Komano, T, Maruyama, Y
Four isogenic strains of temperature-sensitive septationless mutants, whose mutations are located on different genes, were used to study the periods of action of the gene products required for the...
Utsumi, R, Kawamukai, M, Obata, K, Morita, J, Himeno, M, Komano, T
A membrane protein with a molecular weight of 40,000 (40K protein) was induced concurrently with cell filamentation by cyclic AMP (cAMP) in a fic mutant. In the crp mutant and the wild-type strain,...
Komano, T, Inouye, S, Inouye, M
Myxococcus xanthus was pulse-labeled with [3H]thymidine immediately after germination of dimethyl sulfoxide-induced spores. The restriction enzyme digests of the total chromosomal DNA from the...
Phosphorylation and methylation of proteins during Myxococcus xanthus spore formation.
Komano, T, Brown, N, Inouye, S, Inouye, M
Post-translational modification of proteins was examined during the life cycle of Myxococcus xanthus. A specific pattern of protein phosphorylation was observed in vegetative cells. When spore...
Utsumi, R, Nakamoto, Y, Kawamukai, M, Himeno, M, Komano, T
Cyclic AMP (cAMP) inhibited septum formation in Escherichia coli PA3092 and induced cell filamentation at elevated temperatures. This phenomenon was first observed in E. coli PA3092 and is due to a...
Honjo, M, Shibano, Y, Komano, T
The deoxyribonucleic acid (DNA) polymerase activities in Bacillus subtilis strains Marburg 168 (thy-trp2) and D22, a DNA polymerase I-deficient mutant, were measured at various stages of sporulation....
Miyakawa, Y, Komano, T, Maruyama, Y
The relationship between protein synthesis and processes of cell division was studied by using synchronized cells of Bacillus subtilis 168. The addition of chloramphenicol at the beginning of...
Miao, D M, Sakai, H, Okamoto, S, Tanaka, K, Okuda, M, Honda, Y, ...
The replication origin of the broad host-range plasmid RSF1010 contains 3.5 copies of a 20mer iteron sequence that bind specifically to the plasmid-encoded initiator, RepC. Here we demonstrated that...
Tanaka, K, Kino, K, Taguchi, Y, Miao, D M, Honda, Y, Sakai, H, ...
The broad host-range plasmid RSF1010 contains two oppositely oriented priming signals, ssiA and ssiB, for DNA synthesis dependent on the origin of vegetative DNA replication (oriV). If either ssiA or...
Miao, D M, Honda, Y, Tanaka, K, Higashi, A, Nakamura, T, Taguchi, Y, ...
The two single-strand DNA initiation signals, ssiA(RSF1010) and ssiB(RSF1010) of the broad host-range plasmid RSF1010 contain proposed stem-loop structures. Nine single base-change mutations in the...
Honda, Y, Nakamura, T, Tanaka, K, Higashi, A, Sakai, H, Komano, T, ...
Broad host-range plasmid RSF1010 contains in the oriV region two DNA initiation signals, ssiA(RSF1010) and ssiB(RSF1010), which are essential for plasmid replication. Each of ssiA and ssiB could be...
Saito, I, Sugiyama, H, Matsuura, T, Ueda, K, Komano, T
A new procedure for T specific cleavage of DNA fragments utilizing photoreaction with spermine has been described. Irradiation of 3'-[32P]-end-labeled DNA fragments for 10-20 min with a germicidal...
Yamano, Y, Ohyama, K, Komano, T
The nucleotide sequences of chloroplast 5S rRNAs from cell suspension cultures of the liverworts Marchantia polymorpha and Jungermannia subulata were determined. Their nucleotide sequences, 119...
Sequence-specific DNA damage induced by reduced mitomycin C and 7-N-(p-hydroxyphenyl)mitomycin C.
Mitomycin C reduced with sodium borohydride induced the DNA damage at deoxyguanosines preferentially in dinucleotide sequence G-T. The DNA damage produced strand breaks when subsequently heated. The...
Hiasa, H, Sakai, H, Komano, T, Godson, G N
45 mutations (insertion, deletion and base substitution) of the G4 Goric were tested for their functional activity in M13 and R199 in vivo. The critical mutants were also assayed for their ability to...
Nucleotide sequence of the growth hormone gene cDNA from goat Capra hircus L. (Tokara).
Yato, M, Yamano, Y, Oyabayashi, K, Okuno, M, Kioka, N, Manabe, E, ...
Tanaka, K, Sakai, H, Honda, Y, Nakamura, T, Higashi, A, Komano, T
We have structurally and functionally analyzed the cis-elements essential for ColIb-P9 plasmid DNA replication. The putative oriV region encompassed a region of 172 base pairs (bp) located 152 bp...
Choi, Y L, Kawase, S, Nishida, T, Sakai, H, Komano, T, Kawamukai, M, ...
A nicked group II intron and trans-splicing in liverwort, Marchantia polymorpha, chloroplasts.
Kohchi, T, Umesono, K, Ogura, Y, Komine, Y, Nakahigashi, K, Komano, T, ...
The chloroplast gene rps12 for ribosomal protein S12 in a liverwort, Marchantia polymorpha, is split into three exons by two introns, one of which (intron 1) is discontinuous. Exon 1 of rps12 for the...
Komano, T, Kubo, A, Nisioka, T
The IncI alpha plasmid R64 was found to bear a highly mobile DNA segment which was designated as a clustered inversion region (J. Bacteriol. 165, 94-100, 1986). The clustered inversion region...
Sequence specific damage of DNA induced by reducing sugars.
Morita, J, Ueda, K, Nanjo, S, Komano, T
Reducing sugars induced alkali-labile sites in DNA. The DNA reacted with D-fructose 6-phosphate or D-fructose in the presence of Cu2+ was cleaved by the treatment with aqueous piperidine at 90...
Growth and DNA synthesis of bacteriophage phi x174 in a dnaP mutant of Escherichia coli.
phi X174am3trD, a temperature-resistant mutant of bacteriophage phi X174am3, exhibited a reduced ability to grow in a dnaP mutant, Escherichia coli KM107, at the restrictive temperature (43 degrees...
Divergent mRNA transcription in the chloroplast psbB operon.
Kohchi, T, Yoshida, T, Komano, T, Ohyama, K
The genes psbB, psbH, petB and petD for the components in photosystem II and the cytochrome b6/f complex are clustered and co-transcribed in liverwort Marchantia polymorpha chloroplasts. On the...