T. Komatsu

Publication List Details

Period

1985 - 2009

Number

90

Co-Authors

Early phase observations of extremely luminous Type Ia Supernova 2009dc (2009)

Yamanaka, M., Kawabata, K. S., Kinugasa, K., Tanaka, M., Imada, A., Maeda, K., ...

We present early phase observations in optical and near-infrared wavelengths for the extremely luminous Type Ia supernova (SN Ia) 2009dc. The decline rate of the light curve is $\Delta...

Transcutaneous electrical nerve stimulation at the PC-5 and PC-6 acupoints reduced the severity of hypotension after spinal anaesthesia in patients undergoing Caesarean section (2008)

Arai, Y. C. P., Kato, N., Matsura, M., Ito, H., Kandatsu, N., Kurokawa, S., ...

Background Despite prophylactic measures, hypotension remains a common side-effect of spinal anaesthesia for parturients. Electroacupuncture at the Neiguan (PC-6) and Jianshi (PC-5) points influences...

Intra-articular injection of warmed lidocaine improves intraoperative anaesthetic and postoperative analgesic conditions (2006)

Arai, Y.-C. P., Ikeuchi, M., Fukunaga, K., Ueda, W., Kimura, T., Komatsu, T.

Background. Although local anaesthesia for knee arthroscopy is a well-documented procedure, arthroscopy under local anaesthesia is often interrupted because of intolerable discomfort and pain....

Growth hormone acts on the synthesis and secretion of alpha-casein in bovine mammary epithelial cells (2005)

Sakamoto, K., Komatsu, T., Kobayashi, T., Rose, Michael T., Aso, H., Hagino, A., ...

To study the effect of growth hormone (GH) on the functions of mammary epithelia, we examined the effect of GH on the synthesis and secretion of α-casein in a bovine mammary epithelial cell (BMEC)...

Periphyton-based aquaculture production: an ecological approach (2005)

Azim, M.E., Wahab, M.A., Verdegem, M.C.J., Beveridge, M.C.M., Little, D.C., ...

The expansion of aquaculture must be accomplished by promoting an ecological aquaculture model which brings not only the technical aspects of eco-systems design and ecological principles to...

Severe hypertension after stellate ganglion block (2005)

Kimura, T., Nishiwaki, K., Yokota, S., Komatsu, T., Shimada, Y.

Haemodynamic effects may occur after stellate ganglion block (SGB) arising from autonomic imbalance and local anaesthetic infiltration to barosensitive areas. We report seven patients who developed...

Intra-articular injection of warmed lidocaine improves intraoperative anaesthetic and postoperative analgesic conditions (2005)

Arai, Y. -C. P., Ikeuchi, M., Fukunaga, K., Ueda, W., Kimura, T., Komatsu, T.

Background. Although local anaesthesia for knee arthroscopy is a well-documented procedure, arthroscopy under local anaesthesia is often interrupted because of intolerable discomfort and pain....

On the number of solutions of the Diophantine equation of Frobenius - General case (2003)

Komatsu, T.

We determine the number of solutions of the equation $a_1 x_1+a_2 x_2+cdots+a_m x_m=b$ in non-negative integers $x_1$, $x_2$, $dots$, $x_n$. If $m=2$, then the largest $b$ for which no solution...

Evolution and characterization of fluorite-like nano-$SrBi_2Nb_2O_9$ phase in the $SrO-Bi_2O_3-Nb_2O_5-Li_2B_4O_7$ glass system (2003)

Prasad, Syam N, Varma, KBR, Takahashi, Y, Benino, Y, Fujiwara, T, Komatsu, T

Transparent glasses of various compositions in the system $(100−x)Li_2B_4O_7 - x(SrO-Bi_2O_3-Nb_2O_5)$ (where x=10, 20, 30, 40, 50 and 60, in molar ratio) were fabricated via splat quenching...

Evolution and characterization of fluorite-like nano-$SrBi_2Nb_2O_9$ phase in the $SrO-Bi_2O_3-Nb_2O_5-Li_2B_4O_7$ glass system (2003)

Prasad, Syam N, Varma, KBR, Takahashi, Y, Benino, Y, Fujiwara, T, Komatsu, T

Transparent glasses of various compositions in the system $(100−x)Li_2B_4O_7 - x(SrO-Bi_2O_3-Nb_2O_5)$ (where x=10, 20, 30, 40, 50 and 60, in molar ratio) were fabricated via splat quenching...

Tunisian mega benthos from Infra (Posidonia meadows) and circalittoral (Coralligenous) sites (2002)

Ben Mustapha, K., Komatsu, T., Hattour, A., Sammari, Ch., Zarrouk, S, Souissi, A., ...

Plusieurs campagnes marines ciblant la collecte de démosponges marines afin d’étudier leur taxonomie ainsi que leur biogéographie et visant à étudier la phénologie et la distribution de...

Posidonia oceanica L. (Delile) Meadow from Messioua (2002)

Ben Mustapha, K., Komatsu, T., Sammari, Ch., Hattour, A., Zarrouk, S, El Abed, A.

Cette étude intéresse l’écologie benthique du « banc Messioua » (30 MN à l’Est de Zarzis, au Sud de la Tunisie), zone traditionnelle de la pêche aux éponges. Quatre campagnes marines ont...

Formation of a dihydroxy metabolite of phenytoin in human liver microsomes/cytosol: Roles of cytochromes P450 2C9, 2C19, and 3A4 (2000)

Komatsu, T., Yamazaki, H., Asahi, S., Gillam, E. M. J., Guengerich, F. P., Nakajima, M., ...

Formation of four oxidative metabolites from the anticonvulsant drug phenytoin (DPH) catalyzed by human liver microsomal cytochrome P450 (P450) enzymes was determined simultaneously. Under the...

Formation of a dihydroxy metabolite of phenytoin in human liver microsomes/cytosol: Roles of cytochromes P450 2C9, 2C19, and 3A4 (2000)

Komatsu, T., Yamazaki, H., Asahi, S., Gillam, E. M. J., Guengerich, F. P., Nakajima, M., ...

Formation of four oxidative metabolites from the anticonvulsant drug phenytoin (DPH) catalyzed by human liver microsomal cytochrome P450 (P450) enzymes was determined simultaneously. Under the...

Continued fractions and Newton's approximations (1999)

Komatsu, T.

We generalise the relationship between continued fractions and Newton's approximations.

Effect of Copper Content on Glass Formation and Superconductivity in the Bi-Pb-Sr-Ca-Cu-O System. (1998)

Komatsu, T., Sato, R., Meguro, H., Matusita, K., Yamashita, T.

High-T(sub c) superconducting ceramics of formula Bi(0.8)Pb(0.2)SrCaCu(x)O(y) (x=1.5, 1.8 and 2.0) were prepared by using the melt-quenching method, and the effect of copper content on glass...

Analysis and prediction of yellowfin tuna (Thunnus albacares) catch rates of longline fisheries in the western Indian Ocean using a neural network. (1998)

Nishida, T., Komatsu, T.

Strengthening tuna management is one of the primary tasks for the new Indian Ocean Tuna Commission (IOTC). Stock assessment and the prediction of the dynamics of the tuna resources will become...

Nonlinear optical spectra of confined and localized excitons in mesoscopic domain and quasi-2D stacking fault interface in BiI3 crystals (1993)

Akai, I., Kondo, H., Ichida, M., Karasawa, T., Komatsu, T.

Saturation spectra of the excitons confined and localized at several kinds of stacking disorders in layered crystal BiI3 are studied. The quasi-two dimensional exciton localized at the stacking fault...

Nonlinear optical spectra of confined and localized excitons in mesoscopic domain and quasi-2D stacking fault interface in BiI3 crystals (1993)

Akai, I., Kondo, H., Ichida, M., Karasawa, T., Komatsu, T.

Saturation spectra of the excitons confined and localized at several kinds of stacking disorders in layered crystal BiI3 are studied. The quasi-two dimensional exciton localized at the stacking fault...

Nonlinear optical spectra of confined and localized excitons in mesoscopic domain and quasi-2D stacking fault interface in BiI3 crystals (1993)

Akai, I., Kondo, H., Ichida, M., Karasawa, T., Komatsu, T.

Saturation spectra of the excitons confined and localized at several kinds of stacking disorders in layered crystal BiI3 are studied. The quasi-two dimensional exciton localized at the stacking fault...

PRESSURE EFFECT ON THE CURIE TEMPERATURE OF Gd1-xYxCo2 (1988)

Yoshida, H., Komatsu, T., Kaneko, T., Abe, S., Kamigaki, K.

The pressure effects of the Curie temperatures Tc of the intermetallic compounds Gd1-xYxCo2 (x = 0, 0.2, 0.4 and 0.6) are studied at pressures up to 10 kbar. dTc / dP is negative for all the...

PRESSURE EFFECT ON THE CURIE TEMPERATURE OF Gd1-xYxCo2 (1988)

Yoshida, H., Komatsu, T., Kaneko, T., Abe, S., Kamigaki, K.

The pressure effects of the Curie temperatures Tc of the intermetallic compounds Gd1-xYxCo2 (x = 0, 0.2, 0.4 and 0.6) are studied at pressures up to 10 kbar. dTc / dP is negative for all the...

PRESSURE EFFECT ON THE CURIE TEMPERATURE OF Gd1-xYxCo2 (1988)

Yoshida, H., Komatsu, T., Kaneko, T., Abe, S., Kamigaki, K.

The pressure effects of the Curie temperatures Tc of the intermetallic compounds Gd1-xYxCo2 (x = 0, 0.2, 0.4 and 0.6) are studied at pressures up to 10 kbar. dTc / dP is negative for all the...

Chromosomal beta-lactamase of Klebsiella oxytoca, a new class A enzyme that hydrolyzes broad-spectrum beta-lactam antibiotics.

Arakawa, Y, Ohta, M, Kido, N, Mori, M, Ito, H, Komatsu, T, ...

The chromosomally encoded beta-lactamase gene of Klebsiella oxytoca E23004, a strain resistant to cefoperazone and aztreonam, was cloned and expressed in Escherichia coli HB101. The molecular mass...

A novel costimulatory factor for gamma interferon induction found in the livers of mice causes endotoxic shock.

Okamura, H, Nagata, K, Komatsu, T, Tanimoto, T, Nukata, Y, Tanabe, F, ...

Administration of monoclonal anti-CD3 antibody to mice treated with Propionibacterium acnes induced secretion of a high level of gamma interferon (IFN-gamma) into the circulation system, while it...

Therapeutic effects of imipenem-cilastatin on experimental intrauterine infections in rats.

Hashizume, T, Komatsu, T, Okumoto, Y, Ogashiwa, M, Kemi, M, Takase, Z

The therapeutic effects of imipenem-cilastatin (MK-0787-MK-791) on experimental intrauterine infections in progesterone-treated virgin rats and postpartum rats were studied. The relative efficacy of...

Expression of the O9 polysaccharide of Escherichia coli: sequencing of the E. coli O9 rfb gene cluster, characterization of mannosyl transferases, and evidence for an ATP-binding cassette transport system.

Kido, N, Torgov, V I, Sugiyama, T, Uchiya, K, Sugihara, H, Komatsu, T, ...

The rfb gene cluster of Escherichia coli O9 directs the synthesis of the O9-specific polysaccharide which has the structure -->2-alpha-Man-(1-->2)-alpha-Man-(1-->2)-alpha-Man-(1-->3)-alpha-...

In vitro antibacterial activity of SM-1652, a new broad-spectrum cephalosporin with antipseudomonal activity.

Fukasawa, M, Noguchi, H, Okuda, T, Komatsu, T, Yano, K

SM-1652 (sodium 7-[D(-)-alpha-(4-hydroxy-6-methylpyridine-3-carboxamido)-alpha-(4-hydroxyphenyl)acetamido]-3-[(1-methyl-1H-tetrazol-5-yl) thiomethyl]-3-cephem-4-carboxylate) is a new semisynthetic...

Vesicular stomatitis virus infection of the central nervous system activates both innate and acquired immunity.

Bi, Z, Barna, M, Komatsu, T, Reiss, C S

Vesicular stomatitis virus (VSV) causes acute infection of the central nervous system (CNS) when intranasally applied. We have examined cellular inflammatory changes in the CNS following VSV...

Host range phenotype induced by mutations in the internal ribosomal entry site of poliovirus RNA.

Shiroki, K, Ishii, T, Aoki, T, Ota, Y, Yang, W X, Komatsu, T, ...

Most poliovirus strains infect only primates. The host range (HR) of poliovirus is thought to be primarily determined by a cell surface molecule that functions as poliovirus receptor (PVR), since it...

Expression of the cloned Escherichia coli O9 rfb gene in various mutant strains of Salmonella typhimurium.

Sugiyama, T, Kido, N, Komatsu, T, Ohta, M, Kato, N

To investigate the effect of chromosomal mutation on the synthesis of rfe-dependent Escherichia coli O9 lipopolysaccharide (LPS), the cloned E. coli O9 rfb gene was introduced into Salmonella...

Partial deletion of the cloned rfb gene of Escherichia coli O9 results in synthesis of a new O-antigenic lipopolysaccharide.

Kido, N, Ohta, M, Iida, K, Hasegawa, T, Ito, H, Arakawa, Y, ...

The rfb gene, involved in the synthesis of the O-specific polysaccharide (a mannose homopolymer) of Escherichia coli O9 lipopolysaccharide (LPS), was cloned in E. coli K-12 strains. The O9-specific...

Molecular characterization of an Enterobacter cloacae gene (romA) which pleiotropically inhibits the expression of Escherichia coli outer membrane proteins.

Komatsu, T, Ohta, M, Kido, N, Arakawa, Y, Ito, H, Mizuno, T, ...

The introduction of a newly cloned Enterobacter cloacae chromosomal gene romA, into Escherichia coli and E. cloacae resulted in enhancement of resistance to quinolones, beta-lactams, chloramphenicol,...

Mutation in Pseudomonas aeruginosa causing simultaneous defects in penicillin-binding protein 5 and in enzyme activities of penicillin release and D-alanine carboxypeptidase.

Noguchi, H, Fukasawa, M, Komatsu, T, Mitsuhashi, S, Matsuhashi, M

Penicillin-binding protein 5 in Pseudomonas aeruginosa had moderately penicillin-sensitive D-alanine carboxypeptidase activity. As in Escherichia coli, a defect in this enzyme activity was not lethal.

Characterization of a temperature-sensitive influenza B virus mutant defective in neuraminidase.

Shibata, S, Yamamoto-Goshima, F, Maeno, K, Hanaichi, T, Fujita, Y, Nakajima, K, ...

ts5, a temperature-sensitive mutant of influenza B virus, belongs to one of seven recombination groups. When the mutant infected MDCK cells at the nonpermissive temperature (37.5 degrees C),...

Increased resistance to multiple drugs by introduction of the Enterobacter cloacae romA gene into OmpF porin-deficient mutants of Escherichia coli K-12.

Komatsu, T, Ohta, M, Kido, N, Arakawa, Y, Ito, H, Kato, N

Introduction of the romA gene cloned from Enterobacter cloacae into Escherichia coli K-12 resulted in almost complete inhibition of OmpF expression and a concomitant increase in resistance to...

Determinants in the 5' noncoding region of poliovirus Sabin 1 RNA that influence the attenuation phenotype.

Kawamura, N, Kohara, M, Abe, S, Komatsu, T, Tago, K, Arita, M, ...

A number of recombinants between the virulent Mahoney and attenuated Sabin strains of type 1 poliovirus were constructed by using infectious cDNA clones of the two strains. To identify a strong...

Construction of less neurovirulent polioviruses by introducing deletions into the 5' noncoding sequence of the genome.

Iizuka, N, Kohara, M, Hagino-Yamagishi, K, Abe, S, Komatsu, T, Tago, K, ...

Viral attenuation may be due to lowered efficiency of certain steps essential for viral multiplication. For the construction of less neurovirulent strains of poliovirus in vitro, we introduced...

Genetic analysis of the attenuation phenotype of poliovirus type 1.

Omata, T, Kohara, M, Kuge, S, Komatsu, T, Abe, S, Semler, B L, ...

Seven different recombinant viruses from the virulent Mahoney and the attenuated Sabin parental strains of type 1 poliovirus were constructed in vitro by using infectious cDNA clones. Monkey...

A recombinant virus between the Sabin 1 and Sabin 3 vaccine strains of poliovirus as a possible candidate for a new type 3 poliovirus live vaccine strain.

Kohara, M, Abe, S, Komatsu, T, Tago, K, Arita, M, Nomoto, A

Biological tests including the monkey neurovirulence test performed on recombinants between the virulent Mahoney and attenuated Sabin 1 strains of type 1 poliovirus indicated that the genome region...

Biosynthesis of Klebsiella K2 capsular polysaccharide in Escherichia coli HB101 requires the functions of rmpA and the chromosomal cps gene cluster of the virulent strain Klebsiella pneumoniae Chedid (O1:K2).

Arakawa, Y, Ohta, M, Wacharotayankun, R, Mori, M, Kido, N, Ito, H, ...

The genes determining the biosynthesis of type 2 (K2) capsular polysaccharide [3----beta Glc1,4----beta Man(1,3----beta GlcUA) 1,4----alpha Glc1----] of Klebsiella pneumoniae Chedid (O1:K2), which is...

Infectivity titration of hemorrhagic fever with renal syndrome virus: use of immune adherence hemagglutination for detection of virus growth.

Matsuura, Y, Sugiyama, K, Morita, C, Morikawa, S, Shiga, S, Komatsu, T, ...

Serial dilutions of hemorrhagic fever with renal syndrome viruses were inoculated into Vero-E6 cells in microplates. After 2 weeks of incubation, infected cells were disrupted by freezing and...

Mode of action of a new nalidixic acid derivative, AB206.

Nagate, T, Komatsu, T, Izawa, A, Ohmura, S, Namiki, S, Mitsuhashi, S

A new chemotherapeutic agent, AB206, shows potent antibacterial activity against gram-negative bacteria, including most of the nalidixic acid-resistant strains tested. It strongly inhibits...

Antibacterial activity of miloxacin.

Izawa, A, Kisaki, Y, Irie, K, Eda, Y, Nakagome, T, Komatsu, T

The chemotherapeutic properties of miloxacin (5,8-dihydro-5-methoxy-8-oxo-2H-1,3-dioxolo-[4,5-g]quinoline-7-carboxylic acid) have been compared with those of oxolinic acid and nalidixic acid. The in...

Absorption and excretion of miloxacin in mice, rats, and dogs.

Izawa, A, Yoshitake, A, Komatsu, T

Miloxacin, a synthetic antibacterial agent structurally related to oxolinic acid, has a broad spectrum of activity in vitro against gram-negative bacteria and considerable activity in vivo against...

Determination of miloxacin and metabolites in human serum and urine by high-pressure liquid chromatography.

Yoshitake, A, Kawahara, K, Shono, F, Umeda, I, Izawa, A, Komatsu, T

A sensitive and reliable high-pressure liquid chromatography (HPLC) assay for miloxacin and its two principal metabolites, 5,8-dihydro-8-oxo-2H-1,3-dioxolo[4,5-g]quinoline-7-carboxylic acid (M-1) and...

Purification of a factor which provides a costimulatory signal for gamma interferon production.

Nakamura, K, Okamura, H, Nagata, K, Komatsu, T, Tamura, T

A protein factor which induces high levels of gamma interferon (IFN-gamma) in resting splenic nonadherent cells was isolated from the sera of mice with generalized inflammation caused by endotoxic...

PC-904, a Novel Broad-Spectrum Semisynthetic Penicillin with Marked Antipseudomonal Activity: Microbiological Evaluation

Noguchi, H., Eda, Y., Tobiki, H., Nakagome, T., Komatsu, T.

PC-904, sodium 6-{d(−)-α-(4-hydroxy-1,5-naphthyridine-3-carboxamido) phenylacetamido}-penicillanate, is a novel semisynthetic penicillin derivative that possesses a broad spectrum of in vitro and...

Chromosomal beta-lactamase of Klebsiella oxytoca, a new class A enzyme that hydrolyzes broad-spectrum beta-lactam antibiotics.

Arakawa, Y, Ohta, M, Kido, N, Mori, M, Ito, H, Komatsu, T, ...

The chromosomally encoded beta-lactamase gene of Klebsiella oxytoca E23004, a strain resistant to cefoperazone and aztreonam, was cloned and expressed in Escherichia coli HB101. The molecular mass...

A novel costimulatory factor for gamma interferon induction found in the livers of mice causes endotoxic shock.

Okamura, H, Nagata, K, Komatsu, T, Tanimoto, T, Nukata, Y, Tanabe, F, ...

Administration of monoclonal anti-CD3 antibody to mice treated with Propionibacterium acnes induced secretion of a high level of gamma interferon (IFN-gamma) into the circulation system, while it...

Therapeutic effects of imipenem-cilastatin on experimental intrauterine infections in rats.

Hashizume, T, Komatsu, T, Okumoto, Y, Ogashiwa, M, Kemi, M, Takase, Z

The therapeutic effects of imipenem-cilastatin (MK-0787-MK-791) on experimental intrauterine infections in progesterone-treated virgin rats and postpartum rats were studied. The relative efficacy of...

Expression of the O9 polysaccharide of Escherichia coli: sequencing of the E. coli O9 rfb gene cluster, characterization of mannosyl transferases, and evidence for an ATP-binding cassette transport system.

Kido, N, Torgov, V I, Sugiyama, T, Uchiya, K, Sugihara, H, Komatsu, T, ...

The rfb gene cluster of Escherichia coli O9 directs the synthesis of the O9-specific polysaccharide which has the structure -->2-alpha-Man-(1-->2)-alpha-Man-(1-->2)-alpha-Man-(1-->3)-alpha-...

In vitro antibacterial activity of SM-1652, a new broad-spectrum cephalosporin with antipseudomonal activity.

Fukasawa, M, Noguchi, H, Okuda, T, Komatsu, T, Yano, K

SM-1652 (sodium 7-[D(-)-alpha-(4-hydroxy-6-methylpyridine-3-carboxamido)-alpha-(4-hydroxyphenyl)acetamido]-3-[(1-methyl-1H-tetrazol-5-yl) thiomethyl]-3-cephem-4-carboxylate) is a new semisynthetic...

Vesicular stomatitis virus infection of the central nervous system activates both innate and acquired immunity.

Bi, Z, Barna, M, Komatsu, T, Reiss, C S

Vesicular stomatitis virus (VSV) causes acute infection of the central nervous system (CNS) when intranasally applied. We have examined cellular inflammatory changes in the CNS following VSV...

Host range phenotype induced by mutations in the internal ribosomal entry site of poliovirus RNA.

Shiroki, K, Ishii, T, Aoki, T, Ota, Y, Yang, W X, Komatsu, T, ...

Most poliovirus strains infect only primates. The host range (HR) of poliovirus is thought to be primarily determined by a cell surface molecule that functions as poliovirus receptor (PVR), since it...

Expression of the cloned Escherichia coli O9 rfb gene in various mutant strains of Salmonella typhimurium.

Sugiyama, T, Kido, N, Komatsu, T, Ohta, M, Kato, N

To investigate the effect of chromosomal mutation on the synthesis of rfe-dependent Escherichia coli O9 lipopolysaccharide (LPS), the cloned E. coli O9 rfb gene was introduced into Salmonella...

Partial deletion of the cloned rfb gene of Escherichia coli O9 results in synthesis of a new O-antigenic lipopolysaccharide.

Kido, N, Ohta, M, Iida, K, Hasegawa, T, Ito, H, Arakawa, Y, ...

The rfb gene, involved in the synthesis of the O-specific polysaccharide (a mannose homopolymer) of Escherichia coli O9 lipopolysaccharide (LPS), was cloned in E. coli K-12 strains. The O9-specific...

Molecular characterization of an Enterobacter cloacae gene (romA) which pleiotropically inhibits the expression of Escherichia coli outer membrane proteins.

Komatsu, T, Ohta, M, Kido, N, Arakawa, Y, Ito, H, Mizuno, T, ...

The introduction of a newly cloned Enterobacter cloacae chromosomal gene romA, into Escherichia coli and E. cloacae resulted in enhancement of resistance to quinolones, beta-lactams, chloramphenicol,...

Mutation in Pseudomonas aeruginosa causing simultaneous defects in penicillin-binding protein 5 and in enzyme activities of penicillin release and D-alanine carboxypeptidase.

Noguchi, H, Fukasawa, M, Komatsu, T, Mitsuhashi, S, Matsuhashi, M

Penicillin-binding protein 5 in Pseudomonas aeruginosa had moderately penicillin-sensitive D-alanine carboxypeptidase activity. As in Escherichia coli, a defect in this enzyme activity was not lethal.

Characterization of a temperature-sensitive influenza B virus mutant defective in neuraminidase.

Shibata, S, Yamamoto-Goshima, F, Maeno, K, Hanaichi, T, Fujita, Y, Nakajima, K, ...

ts5, a temperature-sensitive mutant of influenza B virus, belongs to one of seven recombination groups. When the mutant infected MDCK cells at the nonpermissive temperature (37.5 degrees C),...

Increased resistance to multiple drugs by introduction of the Enterobacter cloacae romA gene into OmpF porin-deficient mutants of Escherichia coli K-12.

Komatsu, T, Ohta, M, Kido, N, Arakawa, Y, Ito, H, Kato, N

Introduction of the romA gene cloned from Enterobacter cloacae into Escherichia coli K-12 resulted in almost complete inhibition of OmpF expression and a concomitant increase in resistance to...

Determinants in the 5' noncoding region of poliovirus Sabin 1 RNA that influence the attenuation phenotype.

Kawamura, N, Kohara, M, Abe, S, Komatsu, T, Tago, K, Arita, M, ...

A number of recombinants between the virulent Mahoney and attenuated Sabin strains of type 1 poliovirus were constructed by using infectious cDNA clones of the two strains. To identify a strong...

Construction of less neurovirulent polioviruses by introducing deletions into the 5' noncoding sequence of the genome.

Iizuka, N, Kohara, M, Hagino-Yamagishi, K, Abe, S, Komatsu, T, Tago, K, ...

Viral attenuation may be due to lowered efficiency of certain steps essential for viral multiplication. For the construction of less neurovirulent strains of poliovirus in vitro, we introduced...

Genetic analysis of the attenuation phenotype of poliovirus type 1.

Omata, T, Kohara, M, Kuge, S, Komatsu, T, Abe, S, Semler, B L, ...

Seven different recombinant viruses from the virulent Mahoney and the attenuated Sabin parental strains of type 1 poliovirus were constructed in vitro by using infectious cDNA clones. Monkey...

A recombinant virus between the Sabin 1 and Sabin 3 vaccine strains of poliovirus as a possible candidate for a new type 3 poliovirus live vaccine strain.

Kohara, M, Abe, S, Komatsu, T, Tago, K, Arita, M, Nomoto, A

Biological tests including the monkey neurovirulence test performed on recombinants between the virulent Mahoney and attenuated Sabin 1 strains of type 1 poliovirus indicated that the genome region...

Biosynthesis of Klebsiella K2 capsular polysaccharide in Escherichia coli HB101 requires the functions of rmpA and the chromosomal cps gene cluster of the virulent strain Klebsiella pneumoniae Chedid (O1:K2).

Arakawa, Y, Ohta, M, Wacharotayankun, R, Mori, M, Kido, N, Ito, H, ...

The genes determining the biosynthesis of type 2 (K2) capsular polysaccharide [3----beta Glc1,4----beta Man(1,3----beta GlcUA) 1,4----alpha Glc1----] of Klebsiella pneumoniae Chedid (O1:K2), which is...

Infectivity titration of hemorrhagic fever with renal syndrome virus: use of immune adherence hemagglutination for detection of virus growth.

Matsuura, Y, Sugiyama, K, Morita, C, Morikawa, S, Shiga, S, Komatsu, T, ...

Serial dilutions of hemorrhagic fever with renal syndrome viruses were inoculated into Vero-E6 cells in microplates. After 2 weeks of incubation, infected cells were disrupted by freezing and...

Mode of action of a new nalidixic acid derivative, AB206.

Nagate, T, Komatsu, T, Izawa, A, Ohmura, S, Namiki, S, Mitsuhashi, S

A new chemotherapeutic agent, AB206, shows potent antibacterial activity against gram-negative bacteria, including most of the nalidixic acid-resistant strains tested. It strongly inhibits...

Antibacterial activity of miloxacin.

Izawa, A, Kisaki, Y, Irie, K, Eda, Y, Nakagome, T, Komatsu, T

The chemotherapeutic properties of miloxacin (5,8-dihydro-5-methoxy-8-oxo-2H-1,3-dioxolo-[4,5-g]quinoline-7-carboxylic acid) have been compared with those of oxolinic acid and nalidixic acid. The in...

Absorption and excretion of miloxacin in mice, rats, and dogs.

Izawa, A, Yoshitake, A, Komatsu, T

Miloxacin, a synthetic antibacterial agent structurally related to oxolinic acid, has a broad spectrum of activity in vitro against gram-negative bacteria and considerable activity in vivo against...

Determination of miloxacin and metabolites in human serum and urine by high-pressure liquid chromatography.

Yoshitake, A, Kawahara, K, Shono, F, Umeda, I, Izawa, A, Komatsu, T

A sensitive and reliable high-pressure liquid chromatography (HPLC) assay for miloxacin and its two principal metabolites, 5,8-dihydro-8-oxo-2H-1,3-dioxolo[4,5-g]quinoline-7-carboxylic acid (M-1) and...

Purification of a factor which provides a costimulatory signal for gamma interferon production.

Nakamura, K, Okamura, H, Nagata, K, Komatsu, T, Tamura, T

A protein factor which induces high levels of gamma interferon (IFN-gamma) in resting splenic nonadherent cells was isolated from the sera of mice with generalized inflammation caused by endotoxic...

PC-904, a Novel Broad-Spectrum Semisynthetic Penicillin with Marked Antipseudomonal Activity: Microbiological Evaluation

Noguchi, H., Eda, Y., Tobiki, H., Nakagome, T., Komatsu, T.

PC-904, sodium 6-{d(−)-α-(4-hydroxy-1,5-naphthyridine-3-carboxamido) phenylacetamido}-penicillanate, is a novel semisynthetic penicillin derivative that possesses a broad spectrum of in vitro and...

Contribution of the mannan O side-chains to the adjuvant action of lipopolysaccharides.

Ohta, M, Kido, N, Hasegawa, T, Ito, H, Fujii, Y, Arakawa, Y, ...

Klebsiella 03 lipopolysaccharide (LPS) and Escherichia coli 09 LPS were previously shown to have potent adjuvant activities in augmenting antibody response and delayed-type hypersensitivity to...

Immunological unresponsiveness in mice. II. Cellular basis of immunological unresponsiveness induced in foetal and neonatal mice by transfer of human gamma-globulin by the maternal route.

Shinka, S, Komatsu, T, Dohi, Y, Amano, T

The cellular basis of the mechanism of immunological tolerance to human gamma-globulin (H gamma G) induced in foetal and neonatal mice by materno-foetal or materno-neonatal transfer after a single...

Autoantibodies from primary biliary cirrhosis patients with anti-p95c antibodies bind to recombinant p97/VCP and inhibit in vitro nuclear envelope assembly

MIYACHI, K, HIRANO, Y, HORIGOME, T, MIMORI, T, MIYAKAWA, H, ONOZUKA, Y, ...

We have reported previously that p95c, a novel 95-kDa cytosolic protein, was the target of autoantibodies in sera of patients with autoimmune hepatic diseases. We studied 30 sera that were shown...

T cell receptor (TCR) repertoire and function of human epidermal T cells: restricted TCR Vα-Vβ genes are utilized by T cells residing in the lesional epidermis in fixed drug eruption

KOMATSU, T, MORIYA, N, SHIOHARA, T

Human epidermis contains a phenotypically heterogeneous population of T cells. No information, however, is available regarding the TCR repertoire of these T cells and their relevant physiologic and...

Mutation of the nm23 gene, loss of heterozygosity at the nm23 locus and K-ras mutation in ovarian carcinoma: correlation with tumour progression and nm23 gene expression.

Mandai, M., Konishi, I., Komatsu, T., Mori, T., Arao, S., Nomura, H., ...

Alteration of expression levels of the nm23 genes has previously been correlated with metastatic status of ovarian epithelial carcinoma. To elucidate the relevance of the qualitative changes of the...

Expression of vascular endothelial growth factor (VEGF) in epithelial ovarian neoplasms: correlation with clinicopathology and patient survival, and analysis of serum VEGF levels.

Yamamoto, S., Konishi, I., Mandai, M., Kuroda, H., Komatsu, T., Nanbu, K., ...

Vascular endothelial growth factor (VEGF) is known to be produced by various solid tumours and is thought to be involved in microvascular permeability and/or angiogenesis. To examine the relationship...