Honda, T, Miwatani, T, Yabushita, Y, Koike, N, Okada, K
A new method of chemically immobilizing antibody on nylon was developed. The method consists of serial treatments with HCl, polyethylene imine, and maleic anhydride methylvinyl ether copolymer, which...
Taniguchi, T, Fujino, Y, Yamamoto, K, Miwatani, T, Honda, T
The plasmid-encoded structural gene cofA necessary for the production of the major pilin subunit of pilus colonization factor antigen III (CFA/III) of human enterotoxigenic Escherichia coli was...
Nagamune, K, Yamamoto, K, Naka, A, Matsuyama, J, Miwatani, T, Honda, T
Vibrio cholerae produces a cytolytic toxin named El Tor cytolysin/hemolysin which is encoded by the hlyA gene. This cytolysin is produced as a 79-kDa precursor form (pro-HlyA) into the culture...
Minami, A, Hashimoto, S, Abe, H, Arita, M, Taniguchi, T, Honda, T, ...
Vibrio cholerae O1 strains isolated from various sources in Japan over the years 1977 through 1987 were examined to confirm the presence or absence of the cholera enterotoxin (CT) gene and production...
Xu, M, Iida, T, Yamamoto, K, Takarada, Y, Miwatani, T, Honda, T
Simultaneous production of a thermostable direct hemolysin (TDH)-like toxin (TDHx) and a TDH-related hemolysin (TRH)-like toxin (TRHx) by a clinical isolate (strain TH3766) of Kanagawa...
Nishibuchi, M, Doke, S, Toizumi, S, Umeda, T, Yoh, M, Miwatani, T
A vibrio isolated from the intestine of a coastal fish was identified as Vibrio hollisae by its biochemical characteristics. The isolate reacted with the gene probe for the thermostable direct...
Yoh, M, Honda, T, Miwatani, T, Tsunasawa, S, Sakiyama, F
Vibrio hollisae produces a hemolysin (Vh-rTDH) that is related to the thermostable direct hemolysin of Vibrio parahaemolyticus (Vp-TDH). Although both hemolysins are essentially similar biologically...
Okamoto, K, Okamoto, K, Miyama, A, Tsuji, T, Honda, T, Miwatani, T
The ADP-ribosyltransferase activity of polypeptide A1 of cholera toxin and that of Escherichia coli heat-labile enterotoxin (LT) are primarily responsible for the toxic activities of these toxins....
Deoxyribonucleic acid strand breaks during freeze-drying and their repair in Escherichia coli.
Ohnishi, T, Tanaka, Y, Yoh, M, Takeda, Y, Miwatani, T
Freeze-drying of Escherichia coli cells caused strand breaks of deoxyribonucleic acid (DNA) in both radiation-sensitive and -resistant strains. However, in the radiation-resistant strain E. coli B/r...
Honda, T, Nishibuchi, M, Miwatani, T, Kaper, J B
Of 15 Vibrio cholerae non-O1 strains, 2 produced a hemolysin termed NAG-rTDH, which is very similar to the thermostable direct hemolysin of V. parahaemolyticus. These two strains contained DNA...
Induction of mutation in Escherichia coli by freeze-drying.
Tanaka, Y, Yoh, M, Takeda, Y, Miwatani, T
The effect of freeze-drying on phenotypic reversion of amino acid auxotrophy to prototrophy was studied in Escherichia coli. In a radioresistant strain, E. coli H/r 30 (uvr+ exr+), which can repair...
Arita, M, Honda, T, Miwatani, T, Ohmori, K, Takao, T, Shimonishi, Y
The possible production of a heat-stable enterotoxin (Vc-H-ST) by Vibrio cholerae non-O1 serogroup Hakata was investigated, and the purified Vc-H-ST was characterized. It has a unique amino acid...
Purification and characterization of Vibrio metschnikovii cytolysin.
Miyake, M, Honda, T, Miwatani, T
An extracellular cytolysin produced by Vibrio metschnikovii was purified by acid precipitation, phenyl-Sepharose CL-4B chromatography, and rechromatography on a phenyl-Sepharose CL-4B column and...
Honda, T, Ni, Y X, Miwatani, T
A clinical isolate (strain 4037) of Kanagawa phenomenon-negative Vibrio parahaemolyticus was studied. Although the strain was judged to be Kanagawa phenomenon-negative by various conventional tests,...
Isolation of Shiga toxin-resistant Vero cells and their use for easy identification of the toxin.
Kongmuang, U, Honda, T, Miwatani, T
Shiga toxin-resistant Vero cells were isolated by treatment of the cells with nitrosoguanidine. These mutant cells were not affected by Shiga toxin at more than 1 microgram/ml, although the parent...
Honda, T, Wetprasit, N, Arita, M, Miwatani, T
Three monoclonal antibodies (MAbs) to a pilus colonization factor (colonization factor antigen III [CFA/III]) of human enterotoxigenic Escherichia coli (ETEC) were developed and characterized. All of...
Enterotoxicity of El Tor-like hemolysin of non-O1 Vibrio cholerae.
Ichinose, Y, Yamamoto, K, Nakasone, N, Tanabe, M J, Takeda, T, Miwatani, T, ...
The enterotoxicity of an El Tor-like hemolysin purified from non-O1 Vibrio cholerae was investigated. Fluid accumulation was induced by injection of purified hemolysin into the ligated intestinal...
Tsuji, T, Honda, T, Miwatani, T
The effect on nicking (proteolytic cleavage) of heat-labile enterotoxin (LT) on its activities on Chinese hamster ovary (CHO) cells was examined. LT nicked with trypsin induced morphological change...
Honda, T, Sato, M, Nishimura, T, Higashitsutsumi, M, Fukai, K, Miwatani, T
The production of toxins by Aeromonas species was examined by the suckling mouse test, the hemolysin test, and the enzyme-linked immunosorbent assay with anticholera enterotoxin. A factor that was...
Purification and characterization of Vibrio cholerae non-O1 heat-stable enterotoxin.
Arita, M, Takeda, T, Honda, T, Miwatani, T
A toxin which causes rapid fluid accumulation in a suckling mouse assay and which was produced by Vibrio cholerae non-O1 was investigated. The toxin was purified from the culture supernatant of V....
A newly identified hemolysin (NAG-rTDH), which is related to the thermostable direct hemolysin (Vp-TDH) of Vibrio parahaemolyticus produced by non-O1 Vibrio cholerae, was studied. NAG-rTDH was...
Yamamoto, K, Al-Omani, M, Honda, T, Takeda, Y, Miwatani, T
Hemolysin of a non-O1 Vibrio cholerae strain was purified and characterized. The purified hemolysin gave a single protein band on conventional and sodium dodecyl sulfate-gel electrophoresis. Its...
Honda, T, Arita, M, Miwatani, T
Enterotoxigenic Escherichia coli strains were divided into five groups on the basis of their bacterial surface hydrophobicity (Honda et al., FEMS Microbiol. Lett. 17:273-276, 1983). Strains in group...
Yoh, M, Yamamoto, K, Honda, T, Takeda, Y, Miwatani, T
Enterotoxigenic Escherichia coli grown in the presence of lincomycin and tetracycline produced an increased amount of heat-labile enterotoxin (LT). These antibiotics increased the production of not...
Yamamoto, K, Takeda, Y, Miwatani, T, Craig, J P
Cholera-like enterotoxin produced by a non-O1 strain of Vibrio cholerae, S7 (S7 enterotoxin), isolated from human diarrheal stool, was purified, and its physicochemical, biological, and immunological...
Takeda, Y, Honda, T, Sima, H, Tsuji, T, Miwatani, T
Antigenic determinants of cholera enterotoxin (CT) and heat-labile enterotoxin from a human strain (LTh) and a porcine strain (LTp) were analyzed by Ouchterlony double-gel diffusion test against...
Enzyme-linked immunosorbent assay to detect Shiga toxin of Shigella dysenteriae and related toxins.
Kongmuang, U, Honda, T, Miwatani, T
A sandwich enzyme-linked immunosorbent assay (ELISA) was developed for detection of Shiga toxin. Four species of Shigella, Escherichia coli, and Vibro parahaemolyticus were tested for production of...
Nishibuchi, M, Arita, M, Honda, T, Miwatani, T
A commercially available, alkaline-phosphatase-conjugated oligonucleotide probe for detecting the heat-stable enterotoxin gene of Escherichia coli was compared with cloned gene probes by examining E....
Nishibuchi, M, Murakami, A, Arita, M, Jikuya, H, Takano, J, Honda, T, ...
We examined variations in the genes encoding heat-stable enterotoxin (ST) and heat-labile enterotoxin (LT) in 88 strains of Escherichia coli isolated from individuals with traveler's diarrhea to find...
Honda, T, Yoh, M, Kongmuang, U, Miwatani, T
Several systems for enzyme-linked immunosorbent assay (ELISA) of thermostable direct hemolysin (TDH) of Vibrio parahaemolyticus were tested, and single-antibody sandwich ELISA systems gave...
Yoh, M, Narita, I, Honda, T, Miwatani, T, Nishibuchi, M
Ten strains of enterotoxigenic Escherichia coli producing heat-labile enterotoxin (LT) were preserved under 12 different conditions. After 1 month, 9 months, and 3 years of preservation, the cultures...
Honda, T, Sato, M, Miwatani, T
Enzyme-linked immunosorbent assays (ELISAs) with antibodies specific to either cholera enterotoxin (CT) of Vibrio cholerae or heat-labile enterotoxin (LT) of enterotoxigenic Escherichia coli were...
Honda, T, Arita, M, Takeda, Y, Miwatani, T
A total of 2,229 strains of Escherichia coli isolated from subjects with traveller's diarrhea were tested for heat-labile enterotoxin production by the Biken test (Honda et al., J. Clin. Microbiol....
Honda, T, Sornchai, C, Takeda, Y, Miwatani, T
Selective media for Vibrio parahaemolyticus, BTB-Teepol agar and modified arabinose-ammonium sulfate-cholate agar, were modified for use in immunological detection of the thermostable direct...
Honda, T, Samakoses, R, Sornchai, C, Takeda, Y, Miwatani, T
For detection of heat-labile enterotoxin-producing enterotoxigenic Escherichia coli, the staphylococcal coagglutination test reported by Brill et al. (J. Clin. Microbiol. 9:49-55, 1979) was modified...
Immunological methods for detection of Kanagawa phenomenon of Vibrio parahaemolyticus.
Honda, T, Chearskul, S, Takeda, Y, Miwatani, T
A modified Elek test and an immuno-halo test were developed for detection of the thermostable direct hemolysin produced by Kanagawa phenomenon-positive strains of Vibrio parahaemolyticus. The...
Tsukamoto, T, Kinoshita, Y, Taga, S, Takeda, Y, Miwatani, T
The method of passive immune hemolysis of Evans and Evans (Infect. Immun. 16:604-609, 1977) for detection of heat-labile enterotoxin produced by enterotoxigenic Escherichia coli was modified. A total...
Modified Elek test for detection of heat-labile enterotoxin of enterotoxigenic Escherichia coli.
Honda, T, Taga, S, Takeda, Y, Miwatani, T
The Elek test was modified for detection of the heat-labile enterotoxin of enterotoxigenic Escherichia coli. A total of 164 strains of E. coli were tested by the modified Elek test, and the results...
Miyake, M, Honda, T, Miwatani, T
Divalent cations and polysaccharides such as inulin and dextran reversibly inhibited hemolysis of rabbit erythrocytes caused by Vibrio metschnikovii cytolysin. On the basis of the 50% inhibitory...
Nishibuchi, M, Taniguchi, T, Misawa, T, Khaeomanee-Iam, V, Honda, T, Miwatani, T
Vibrio parahaemolyticus isolates derived from an outbreak of gastroenteritis in the Republic of Maldives did not have the genetic potential to produce the thermostable direct hemolysin, but one such...
Honda, T, Lertpocasombat, K, Hata, A, Miwatani, T, Finkelstein, R A
A protease produced by a clinical isolate of Vibrio cholerae non-O1 was purified to apparent homogeneity by ammonium sulfate fractionation and successive column chromatography on DEAE-Sephadex A25,...
Yamamoto, K, Ichinose, Y, Shinagawa, H, Makino, K, Nakata, A, Iwanaga, M, ...
Vibrio cholerae O1 biotype El Tor produces and secretes a 65-kDa cytolysin/hemolysin into the culture medium. We cloned the structural gene (hlyA) for the cytolysin from the total DNA of a V....
Tsuji, T, Taga, S, Honda, T, Takeda, Y, Miwatani, T
The heat-labile enterotoxins produced by human enterotoxigenic Escherichia coli (LTh) and porcine enterotoxigenic E. coli (LTp) were purified to homogeneity, and their molecular properties were...
Yamamoto, K, Takeda, Y, Miwatani, T, Craig, J P
Cholera-like enterotoxin was isolated and purified from the culture supernatant of a non-O1 strain of Vibrio cholerae, E8498, isolated from the environment. Enterotoxin was purified by aluminum...
Vascular permeability activity in Escherichia coli heat-stable enterotoxin.
Craig, J P, Yamamoto, K, Takeda, T, Takeda, Y, Miwatani, T
Purified heat-stable enterotoxin (ST) prepared from supernatants of Escherichia coli strain 53402 A-1, isolated from a patient with diarrhea, caused an increase in the permeability of the small blood...
Craig, J P, Yamamoto, K, Takeda, Y, Miwatani, T
Vibrio cholerae non-O1 strain E8498, isolated in 1978 from fresh water in Louisiana, produced a vascular permeability factor when cultured in shallow resting cultures of Casamino Acids-yeast...
Honda, T, Takeda, Y, Miwatani, T
Cholera enterotoxin (CT) and heat-labile enterotoxin (LT) produced by enterotoxigenic Escherichia coli share a common antigenic determinant. In addition, CT and LT each have a unique antigenic...
Honda, T, Tsuji, T, Takeda, Y, Miwatani, T
The Ouchterlony double-gel diffusion test demonstrated that heat-labile enterotoxin (LT) from human strains of enterotoxigenic Escherichia coli was antigenically related but not identical to that...
Takeda, Y, Honda, T, Taga, S, Miwatani, T
Heat-labile enterotoxin (LT) was purified from cells of enterotoxigenic Escherichia coli isolated from a patient with traveller's diarrhea. Purified LT was separated into A and B subunits by...
Takeda, T, Honda, T, Takeda, Y, Miwatani, T
We studied the effect of chlorpromazine on fluid accumulation caused by purified heat-stable enterotoxin (ST) from enterotoxigenic Escherichia coli in suckling mice. We found that chlorpromazine...
Detection of cholera enterotoxin activity in suckling hamsters.
Takeda, T, Takeda, Y, Miwatani, T, Ohtomo, N
For detection of cholera enterotoxin activity, suckling hamster, mouse, and rat models were compared. It was found that not only suckling mice but also suckling rats and hamsters were sensitive to...
Takeda, Y, Takeda, T, Yano, T, Yamamoto, K, Miwatani, T
Heat-stable enterotoxin was purified from a strain of enterotoxigenic Escherichia coli 53402 A-1 from human intestine. The cells were cultured in Casamino Acids-yeast extract-salts medium, and the...
Takeda, Y, Hori, Y, Taga, S, Sakurai, J, Miwatani, T
The termostable direct hemolysin of Vibrio parahaemolyticus is inactivated by heating at around 55 C with an inactivating factor isolated from culture filtrates of V. parahaemolyticus. The...
Honda, T, Shimizu, M, Takeda, Y, Miwatani, T
A factor changing Chinese hamster ovary cells from an oval to a spindle shape was isolated from the culture filtrate of Vibrio parahaemolyticus. It was partially purified by successive column...
Honda, T, Taga, S, Takeda, T, Hasibuan, M A, Takeda, Y, Miwatani, T
Lethal toxin was purified extensively from the culture filtrate of a Kanagawa phenomenon-positive strain of Vibrio parahaemolyticus. The purified toxin was a protein, and its homogeneity was...
Honda, T, Goshima, K, Takeda, Y, Sugino, Y, Miwatani, T
Intravenous injection of the thermostable direct hemolysin (lethal toxin) produced by Vibrio parahaemolyticus caused rapid death of rats. Studies by electroencephalography and electrocardiography...
Sakurai, J, Honda, T, Jinguji, Y, Arita, M, Miwatani, T
The thermostable direct hemolysin produced by Vibrio parahaemolyticus showed cytotoxic activity on FL cells derived from human amniotic membrane. Scanning electron micrographs of the whole cells...
Takeda, Y, Takeda, T, Honda, T, Miwatani, T
Biological activities of the thermostable direct hemolysin produced by Vibrio parahaemolyticus, such as its hemolytic activity and lethal activity, were inhibited by neuraminidase-sensitive...
Takeda, Y, Ogiso, Y, Miwatani, T
The effect of Zn2+ on hemolysis induced by the thermostable direct hemolysin from Vibrio parahaemolyticus (vibriolysin) streptolysin O, and Triton X-100 was studied. We found that in certain buffers,...
Takeda, Y, Okamoto, K, Miwatani, T
A toxin that causes morphological changes in Chinese hamster ovary cells was isolated from a culture filtrate of Shigella dysenteriae 1. It was partially purified by successive column chromatography...
Takeda, Y, Okamoto, K, Miwatani, T
Shigella dysenteriae produced a toxin that caused morphological changes in Chinese hamster ovary cells which was different from the neurotoxin (Y. Takeda, K. Okamoto, and T. Miwatani, Infect. Immun....
Honda, T, Miwatani, T, Yabushita, Y, Koike, N, Okada, K
A new method of chemically immobilizing antibody on nylon was developed. The method consists of serial treatments with HCl, polyethylene imine, and maleic anhydride methylvinyl ether copolymer, which...
Taniguchi, T, Fujino, Y, Yamamoto, K, Miwatani, T, Honda, T
The plasmid-encoded structural gene cofA necessary for the production of the major pilin subunit of pilus colonization factor antigen III (CFA/III) of human enterotoxigenic Escherichia coli was...
Nagamune, K, Yamamoto, K, Naka, A, Matsuyama, J, Miwatani, T, Honda, T
Vibrio cholerae produces a cytolytic toxin named El Tor cytolysin/hemolysin which is encoded by the hlyA gene. This cytolysin is produced as a 79-kDa precursor form (pro-HlyA) into the culture...
Minami, A, Hashimoto, S, Abe, H, Arita, M, Taniguchi, T, Honda, T, ...
Vibrio cholerae O1 strains isolated from various sources in Japan over the years 1977 through 1987 were examined to confirm the presence or absence of the cholera enterotoxin (CT) gene and production...
Xu, M, Iida, T, Yamamoto, K, Takarada, Y, Miwatani, T, Honda, T
Simultaneous production of a thermostable direct hemolysin (TDH)-like toxin (TDHx) and a TDH-related hemolysin (TRH)-like toxin (TRHx) by a clinical isolate (strain TH3766) of Kanagawa...
Nishibuchi, M, Doke, S, Toizumi, S, Umeda, T, Yoh, M, Miwatani, T
A vibrio isolated from the intestine of a coastal fish was identified as Vibrio hollisae by its biochemical characteristics. The isolate reacted with the gene probe for the thermostable direct...
Yoh, M, Honda, T, Miwatani, T, Tsunasawa, S, Sakiyama, F
Vibrio hollisae produces a hemolysin (Vh-rTDH) that is related to the thermostable direct hemolysin of Vibrio parahaemolyticus (Vp-TDH). Although both hemolysins are essentially similar biologically...
Okamoto, K, Okamoto, K, Miyama, A, Tsuji, T, Honda, T, Miwatani, T
The ADP-ribosyltransferase activity of polypeptide A1 of cholera toxin and that of Escherichia coli heat-labile enterotoxin (LT) are primarily responsible for the toxic activities of these toxins....
Deoxyribonucleic acid strand breaks during freeze-drying and their repair in Escherichia coli.
Ohnishi, T, Tanaka, Y, Yoh, M, Takeda, Y, Miwatani, T
Freeze-drying of Escherichia coli cells caused strand breaks of deoxyribonucleic acid (DNA) in both radiation-sensitive and -resistant strains. However, in the radiation-resistant strain E. coli B/r...
Honda, T, Nishibuchi, M, Miwatani, T, Kaper, J B
Of 15 Vibrio cholerae non-O1 strains, 2 produced a hemolysin termed NAG-rTDH, which is very similar to the thermostable direct hemolysin of V. parahaemolyticus. These two strains contained DNA...
Induction of mutation in Escherichia coli by freeze-drying.
Tanaka, Y, Yoh, M, Takeda, Y, Miwatani, T
The effect of freeze-drying on phenotypic reversion of amino acid auxotrophy to prototrophy was studied in Escherichia coli. In a radioresistant strain, E. coli H/r 30 (uvr+ exr+), which can repair...
Arita, M, Honda, T, Miwatani, T, Ohmori, K, Takao, T, Shimonishi, Y
The possible production of a heat-stable enterotoxin (Vc-H-ST) by Vibrio cholerae non-O1 serogroup Hakata was investigated, and the purified Vc-H-ST was characterized. It has a unique amino acid...
Purification and characterization of Vibrio metschnikovii cytolysin.
Miyake, M, Honda, T, Miwatani, T
An extracellular cytolysin produced by Vibrio metschnikovii was purified by acid precipitation, phenyl-Sepharose CL-4B chromatography, and rechromatography on a phenyl-Sepharose CL-4B column and...
Honda, T, Ni, Y X, Miwatani, T
A clinical isolate (strain 4037) of Kanagawa phenomenon-negative Vibrio parahaemolyticus was studied. Although the strain was judged to be Kanagawa phenomenon-negative by various conventional tests,...
Isolation of Shiga toxin-resistant Vero cells and their use for easy identification of the toxin.
Kongmuang, U, Honda, T, Miwatani, T
Shiga toxin-resistant Vero cells were isolated by treatment of the cells with nitrosoguanidine. These mutant cells were not affected by Shiga toxin at more than 1 microgram/ml, although the parent...
Honda, T, Wetprasit, N, Arita, M, Miwatani, T
Three monoclonal antibodies (MAbs) to a pilus colonization factor (colonization factor antigen III [CFA/III]) of human enterotoxigenic Escherichia coli (ETEC) were developed and characterized. All of...
Enterotoxicity of El Tor-like hemolysin of non-O1 Vibrio cholerae.
Ichinose, Y, Yamamoto, K, Nakasone, N, Tanabe, M J, Takeda, T, Miwatani, T, ...
The enterotoxicity of an El Tor-like hemolysin purified from non-O1 Vibrio cholerae was investigated. Fluid accumulation was induced by injection of purified hemolysin into the ligated intestinal...
Tsuji, T, Honda, T, Miwatani, T
The effect on nicking (proteolytic cleavage) of heat-labile enterotoxin (LT) on its activities on Chinese hamster ovary (CHO) cells was examined. LT nicked with trypsin induced morphological change...
Honda, T, Sato, M, Nishimura, T, Higashitsutsumi, M, Fukai, K, Miwatani, T
The production of toxins by Aeromonas species was examined by the suckling mouse test, the hemolysin test, and the enzyme-linked immunosorbent assay with anticholera enterotoxin. A factor that was...
Purification and characterization of Vibrio cholerae non-O1 heat-stable enterotoxin.
Arita, M, Takeda, T, Honda, T, Miwatani, T
A toxin which causes rapid fluid accumulation in a suckling mouse assay and which was produced by Vibrio cholerae non-O1 was investigated. The toxin was purified from the culture supernatant of V....
A newly identified hemolysin (NAG-rTDH), which is related to the thermostable direct hemolysin (Vp-TDH) of Vibrio parahaemolyticus produced by non-O1 Vibrio cholerae, was studied. NAG-rTDH was...
Yamamoto, K, Al-Omani, M, Honda, T, Takeda, Y, Miwatani, T
Hemolysin of a non-O1 Vibrio cholerae strain was purified and characterized. The purified hemolysin gave a single protein band on conventional and sodium dodecyl sulfate-gel electrophoresis. Its...
Honda, T, Arita, M, Miwatani, T
Enterotoxigenic Escherichia coli strains were divided into five groups on the basis of their bacterial surface hydrophobicity (Honda et al., FEMS Microbiol. Lett. 17:273-276, 1983). Strains in group...
Yoh, M, Yamamoto, K, Honda, T, Takeda, Y, Miwatani, T
Enterotoxigenic Escherichia coli grown in the presence of lincomycin and tetracycline produced an increased amount of heat-labile enterotoxin (LT). These antibiotics increased the production of not...
Yamamoto, K, Takeda, Y, Miwatani, T, Craig, J P
Cholera-like enterotoxin produced by a non-O1 strain of Vibrio cholerae, S7 (S7 enterotoxin), isolated from human diarrheal stool, was purified, and its physicochemical, biological, and immunological...
Takeda, Y, Honda, T, Sima, H, Tsuji, T, Miwatani, T
Antigenic determinants of cholera enterotoxin (CT) and heat-labile enterotoxin from a human strain (LTh) and a porcine strain (LTp) were analyzed by Ouchterlony double-gel diffusion test against...
Enzyme-linked immunosorbent assay to detect Shiga toxin of Shigella dysenteriae and related toxins.
Kongmuang, U, Honda, T, Miwatani, T
A sandwich enzyme-linked immunosorbent assay (ELISA) was developed for detection of Shiga toxin. Four species of Shigella, Escherichia coli, and Vibro parahaemolyticus were tested for production of...
Nishibuchi, M, Arita, M, Honda, T, Miwatani, T
A commercially available, alkaline-phosphatase-conjugated oligonucleotide probe for detecting the heat-stable enterotoxin gene of Escherichia coli was compared with cloned gene probes by examining E....
Nishibuchi, M, Murakami, A, Arita, M, Jikuya, H, Takano, J, Honda, T, ...
We examined variations in the genes encoding heat-stable enterotoxin (ST) and heat-labile enterotoxin (LT) in 88 strains of Escherichia coli isolated from individuals with traveler's diarrhea to find...
Honda, T, Yoh, M, Kongmuang, U, Miwatani, T
Several systems for enzyme-linked immunosorbent assay (ELISA) of thermostable direct hemolysin (TDH) of Vibrio parahaemolyticus were tested, and single-antibody sandwich ELISA systems gave...
Yoh, M, Narita, I, Honda, T, Miwatani, T, Nishibuchi, M
Ten strains of enterotoxigenic Escherichia coli producing heat-labile enterotoxin (LT) were preserved under 12 different conditions. After 1 month, 9 months, and 3 years of preservation, the cultures...
Honda, T, Sato, M, Miwatani, T
Enzyme-linked immunosorbent assays (ELISAs) with antibodies specific to either cholera enterotoxin (CT) of Vibrio cholerae or heat-labile enterotoxin (LT) of enterotoxigenic Escherichia coli were...
Honda, T, Arita, M, Takeda, Y, Miwatani, T
A total of 2,229 strains of Escherichia coli isolated from subjects with traveller's diarrhea were tested for heat-labile enterotoxin production by the Biken test (Honda et al., J. Clin. Microbiol....
Honda, T, Sornchai, C, Takeda, Y, Miwatani, T
Selective media for Vibrio parahaemolyticus, BTB-Teepol agar and modified arabinose-ammonium sulfate-cholate agar, were modified for use in immunological detection of the thermostable direct...
Honda, T, Samakoses, R, Sornchai, C, Takeda, Y, Miwatani, T
For detection of heat-labile enterotoxin-producing enterotoxigenic Escherichia coli, the staphylococcal coagglutination test reported by Brill et al. (J. Clin. Microbiol. 9:49-55, 1979) was modified...
Immunological methods for detection of Kanagawa phenomenon of Vibrio parahaemolyticus.
Honda, T, Chearskul, S, Takeda, Y, Miwatani, T
A modified Elek test and an immuno-halo test were developed for detection of the thermostable direct hemolysin produced by Kanagawa phenomenon-positive strains of Vibrio parahaemolyticus. The...
Tsukamoto, T, Kinoshita, Y, Taga, S, Takeda, Y, Miwatani, T
The method of passive immune hemolysis of Evans and Evans (Infect. Immun. 16:604-609, 1977) for detection of heat-labile enterotoxin produced by enterotoxigenic Escherichia coli was modified. A total...
Modified Elek test for detection of heat-labile enterotoxin of enterotoxigenic Escherichia coli.
Honda, T, Taga, S, Takeda, Y, Miwatani, T
The Elek test was modified for detection of the heat-labile enterotoxin of enterotoxigenic Escherichia coli. A total of 164 strains of E. coli were tested by the modified Elek test, and the results...
Miyake, M, Honda, T, Miwatani, T
Divalent cations and polysaccharides such as inulin and dextran reversibly inhibited hemolysis of rabbit erythrocytes caused by Vibrio metschnikovii cytolysin. On the basis of the 50% inhibitory...
Nishibuchi, M, Taniguchi, T, Misawa, T, Khaeomanee-Iam, V, Honda, T, Miwatani, T
Vibrio parahaemolyticus isolates derived from an outbreak of gastroenteritis in the Republic of Maldives did not have the genetic potential to produce the thermostable direct hemolysin, but one such...
Honda, T, Lertpocasombat, K, Hata, A, Miwatani, T, Finkelstein, R A
A protease produced by a clinical isolate of Vibrio cholerae non-O1 was purified to apparent homogeneity by ammonium sulfate fractionation and successive column chromatography on DEAE-Sephadex A25,...
Yamamoto, K, Ichinose, Y, Shinagawa, H, Makino, K, Nakata, A, Iwanaga, M, ...
Vibrio cholerae O1 biotype El Tor produces and secretes a 65-kDa cytolysin/hemolysin into the culture medium. We cloned the structural gene (hlyA) for the cytolysin from the total DNA of a V....
Tsuji, T, Taga, S, Honda, T, Takeda, Y, Miwatani, T
The heat-labile enterotoxins produced by human enterotoxigenic Escherichia coli (LTh) and porcine enterotoxigenic E. coli (LTp) were purified to homogeneity, and their molecular properties were...
Yamamoto, K, Takeda, Y, Miwatani, T, Craig, J P
Cholera-like enterotoxin was isolated and purified from the culture supernatant of a non-O1 strain of Vibrio cholerae, E8498, isolated from the environment. Enterotoxin was purified by aluminum...
Vascular permeability activity in Escherichia coli heat-stable enterotoxin.
Craig, J P, Yamamoto, K, Takeda, T, Takeda, Y, Miwatani, T
Purified heat-stable enterotoxin (ST) prepared from supernatants of Escherichia coli strain 53402 A-1, isolated from a patient with diarrhea, caused an increase in the permeability of the small blood...
Craig, J P, Yamamoto, K, Takeda, Y, Miwatani, T
Vibrio cholerae non-O1 strain E8498, isolated in 1978 from fresh water in Louisiana, produced a vascular permeability factor when cultured in shallow resting cultures of Casamino Acids-yeast...
Honda, T, Takeda, Y, Miwatani, T
Cholera enterotoxin (CT) and heat-labile enterotoxin (LT) produced by enterotoxigenic Escherichia coli share a common antigenic determinant. In addition, CT and LT each have a unique antigenic...
Honda, T, Tsuji, T, Takeda, Y, Miwatani, T
The Ouchterlony double-gel diffusion test demonstrated that heat-labile enterotoxin (LT) from human strains of enterotoxigenic Escherichia coli was antigenically related but not identical to that...
Takeda, Y, Honda, T, Taga, S, Miwatani, T
Heat-labile enterotoxin (LT) was purified from cells of enterotoxigenic Escherichia coli isolated from a patient with traveller's diarrhea. Purified LT was separated into A and B subunits by...
Takeda, T, Honda, T, Takeda, Y, Miwatani, T
We studied the effect of chlorpromazine on fluid accumulation caused by purified heat-stable enterotoxin (ST) from enterotoxigenic Escherichia coli in suckling mice. We found that chlorpromazine...
Detection of cholera enterotoxin activity in suckling hamsters.
Takeda, T, Takeda, Y, Miwatani, T, Ohtomo, N
For detection of cholera enterotoxin activity, suckling hamster, mouse, and rat models were compared. It was found that not only suckling mice but also suckling rats and hamsters were sensitive to...
Takeda, Y, Takeda, T, Yano, T, Yamamoto, K, Miwatani, T
Heat-stable enterotoxin was purified from a strain of enterotoxigenic Escherichia coli 53402 A-1 from human intestine. The cells were cultured in Casamino Acids-yeast extract-salts medium, and the...
Takeda, Y, Hori, Y, Taga, S, Sakurai, J, Miwatani, T
The termostable direct hemolysin of Vibrio parahaemolyticus is inactivated by heating at around 55 C with an inactivating factor isolated from culture filtrates of V. parahaemolyticus. The...
Honda, T, Shimizu, M, Takeda, Y, Miwatani, T
A factor changing Chinese hamster ovary cells from an oval to a spindle shape was isolated from the culture filtrate of Vibrio parahaemolyticus. It was partially purified by successive column...
Honda, T, Taga, S, Takeda, T, Hasibuan, M A, Takeda, Y, Miwatani, T
Lethal toxin was purified extensively from the culture filtrate of a Kanagawa phenomenon-positive strain of Vibrio parahaemolyticus. The purified toxin was a protein, and its homogeneity was...
Honda, T, Goshima, K, Takeda, Y, Sugino, Y, Miwatani, T
Intravenous injection of the thermostable direct hemolysin (lethal toxin) produced by Vibrio parahaemolyticus caused rapid death of rats. Studies by electroencephalography and electrocardiography...
Sakurai, J, Honda, T, Jinguji, Y, Arita, M, Miwatani, T
The thermostable direct hemolysin produced by Vibrio parahaemolyticus showed cytotoxic activity on FL cells derived from human amniotic membrane. Scanning electron micrographs of the whole cells...
Takeda, Y, Takeda, T, Honda, T, Miwatani, T
Biological activities of the thermostable direct hemolysin produced by Vibrio parahaemolyticus, such as its hemolytic activity and lethal activity, were inhibited by neuraminidase-sensitive...
Takeda, Y, Ogiso, Y, Miwatani, T
The effect of Zn2+ on hemolysis induced by the thermostable direct hemolysin from Vibrio parahaemolyticus (vibriolysin) streptolysin O, and Triton X-100 was studied. We found that in certain buffers,...
Takeda, Y, Okamoto, K, Miwatani, T
A toxin that causes morphological changes in Chinese hamster ovary cells was isolated from a culture filtrate of Shigella dysenteriae 1. It was partially purified by successive column chromatography...
Takeda, Y, Okamoto, K, Miwatani, T
Shigella dysenteriae produced a toxin that caused morphological changes in Chinese hamster ovary cells which was different from the neurotoxin (Y. Takeda, K. Okamoto, and T. Miwatani, Infect. Immun....
Kurotobi, S., Sano, T., Matsushita, T., Takeuchi, M., Kogaki, S., Miwatani, T., ...
OBJECTIVE: To determine whether the proximal isovelocity surface area (PISA) method could be applied to estimate the magnitude of ventricular septal defect (VSD) shunt flow. DESIGN: Prospective...