T. Okazaki

Publication List Details

Period

1987 - 2008

Number

139

Co-Authors

Synthesis of carbon nanotube peapods directly on Si substrates (2008)

Y. Ohno, B Y. Kurokawa, S. Kishimoto, T. Mizutani, T. Shimada, M. Ishida, ...

1 Carbon nanotubes encapsulating fullerenes, peapods, have been synthesized directly on Si substrates. High-density and high-quality singe-walled carbon nanotubes (SWNTs) were grown on a SiO2/Si...

Atomic-like behaviors and orbital-related Luttinger liquid in carbon nano-peapod quantum dots (2007)

Haruyama, J., Mizubayashi, J., Takesue, I., Okazaki, T., Shinohara, H., Harada, Y., ...

We report influence of encapsulated C60 molecules on electron transport in carbon-nanotube peapod quantum dots. We find atomic-like behaviors with doubly degenerate electronic levels, which exist...

Anomalous Coulomb diamonds and power-law behavior sensitive to back-gate voltages in carbon nanoscale peapod quantum dots (2007)

Mizubayashi, J., Haruyama, J., Takesue, I., Okazaki, T., Shinohara, H., Harada, Y., ...

We report anomalous charging effect of single electrons (Coulomb diamonds) observed in carbon nanoscale peapod quantum dots that encapsulate a series of C60 molecules. We find that behaviors of...

Atomic-like behaviors and orbital-related Luttinger liquid in carbon nano-peapod quantum dots (2006)

Haruyama, J., Mizubayashi, J., Takesue, I., Okazaki, T., Shinohara, Y., Harada, Y., ...

We report influence of encapsulated C60 molecules on electron transport in carbon-nanotube peapod quantum dots. We find atomic-like behaviors with doubly degenerate electronic levels, which exist...

Atomic-like behaviors and orbital-related Luttinger liquid in carbon nano-peapod quantum dots (2006)

Haruyama, J., Mizubayashi, J., Takesue, I., Okazaki, T., Shinohara, Y., Harada, Y., ...

We report influence of encapsulated C60 molecules on electron transport in carbon-nanotube peapod quantum dots. We find atomic-like behaviors with doubly degenerate electronic levels, which exist...

Atomic-like behaviors and orbital-related Tomonaga-Luttinger liquid in peapod quantum dots (2005)

Mizubayashi, J., Haruyama, J., Takesue, I., Okazaki, T., Shinohara, H., Harada, N., ...

We report encapsulated C60 molecules on electron transport in carbon-nanotube peapod quantum dots. We find atomic-like behaviors with doubly degenerate electronic levels, which exist only around...

Synthesis of carbon nanotube peapods directly on Si substrates (2005)

Ohno, Y., Kurokawa, Y., Kishimoto, S., Mizutani, T., Shimada, T., Ishida, M., ...

Carbon nanotubes encapsulating fullerenes, peapods, have been synthesized directly on Si substrates. High-density and high-quality singe-walled carbon nanotubes (SWNTs) were grown on a SiO_2/Si...

Atomic-like behaviors and orbital-related Luttinger liquid in carbon nano-peapod quantum dots (2005)

Haruyama, J., Mizubayashi, J., Takesue, I., Okazaki, T., Shinohara, H., Harada, Y., ...

We report influence of encapsulated C60 molecules on electron transport in carbon-nanotube peapod quantum dots. We find atomic-like behaviors with doubly degenerate electronic levels, which exist...

Atomic-like behaviors and orbital-related Luttinger liquid in carbon nano-peapod quantum dots (2005)

Haruyama, J., Mizubayashi, J., Takesue, I., Okazaki, T., Shinohara, H., Harada, Y., ...

We report influence of encapsulated C60 molecules on electron transport in carbon-nanotube peapod quantum dots. We find atomic-like behaviors with doubly degenerate electronic levels, which exist...

Ferromagnetic shape Memory of Nanostructure Fe-Pd Alloy: The Texture Observation Study by Laser and Electronic Microscopes (2003)

Okazaki, T., Kubota, T., Furuya, Y., Kajiwara, S., Kikuchi, T.

Ferromagnetic shape memory Fe-29.6at%Pd alloy ribbon (about 60 micrometer thickness) prepared by the rapid solidification melt-spinning method has a large magnetostriction of 1.0 x 10(exp -3) when a...

Ambipolar field-effect transistor behavior of Gd@C_{82} metallofullerene peapods (2002)

Shimada, T., Okazaki, T., Taniguchi, R., Sugai, T., Shinohara, H., Suenaga, K., ...

Transport properties of C_60 fullerene peapods and Gd@C_82 metallofullerene peapods are investigated by using the field effect in a gated structure. The results show that C_60 peapods exhibit...

Dwarf galaxy formation induced by galaxy interactions (2000)

Okazaki, T., Taniguchi, Y.

Growing evidence has been accumulated for that some gas-rich dwarf galaxies are formed from material liberated by galaxy collisions and/or mergers. Also, gas-poor dwarf elliptical galaxies are often...

Enhanced expression of vascular endothelial growth factor in lungs of newborn infants with congenital diaphragmatic hernia and pulmonary hypertension (1999)

Shehata, S.M., Mooi, W.J., Okazaki, T., El-Banna, I., Sharma, H.S., Tibboel, D.

BACKGROUND: Pulmonary hypoplasia accompanied by pulmonary hypertension resistant to treatment is an important feature of congenital diaphragmatic hernia (CDH). The pathogenesis of the pulmonary...

New Development of Rapid-Solidified Fe-Ga Sensor/Actuator Alloy (1998)

Okazaki, T., Furuya, Y., Saito, C., Watanabe, T., Wuttig, M.

Rapid-solidification method was applied to make Fe-15at%Ga and Fe-17at%Ga ribbons of 100 micrometers thickness. These ribbons have large magnetostriction of 270 ppm, where the coercive force exhibits...

Development of Texture-Controlled Bulk-Materials by Combing Rapid-Solidified Fiber/Ribbon Material Elements with Spark Plasma Sintering(SPS) (1998)

Furuya, Y., Yokoyama, M., Saito, C., Okazaki, T., Omori, M.

It has been shown that rapid-solidified (RS) melt-spun fiber/ribbon/foil samples can have very high performance of the metallic actuator/sensor properties. However, as for the applications of these...

Equivalence between Schwinger and Dirac schemes of quantization (1997)

Ogawa, N., Fujii, K., Miyazaki, H., Chepilko, N., Okazaki, T.

This paper introduces the modified version of Schwinger's quantization method, in which the information on constraints and the choice of gauge conditions are included implicitly in the choice of...

Study of Local Structures of Ni-Mn Alloys by EXAFS (1997)

Nakahata, M., Yuza, M., Kondo, Y., Miyanaga, T., Okazaki, T.

EXAFS spectra of NiMn alloys annealed at various conditions have been measured at Ni and Mn K-edges. A simple method to estimate the degree of short-range order of atomic arrangements in NiMn alloys...

Study of Local Structures of Ni-Mn Alloys by EXAFS (1997)

Nakahata, M., Yuza, M., Kondo, Y., Miyanaga, T., Okazaki, T.

EXAFS spectra of NiMn alloys annealed at various conditions have been measured at Ni and Mn K-edges. A simple method to estimate the degree of short-range order of atomic arrangements in NiMn alloys...

Study of Local Structures of Ni-Mn Alloys by EXAFS (1997)

Nakahata, M., Yuza, M., Kondo, Y., Miyanaga, T., Okazaki, T.

EXAFS spectra of NiMn alloys annealed at various conditions have been measured at Ni and Mn K-edges. A simple method to estimate the degree of short-range order of atomic arrangements in NiMn alloys...

The deformation of the interacting nucleon in the Skyrme model (1996)

Rahimov, A., Okazaki, T., Musakhanov, M. M., Khanna, F. C.

Changes in the nucleon shape are investigated by letting the nucleon deform under the strong interactions with another nucleon. The parameters of the axial deformations are obtained by minimizing the...

BONSAI Garden: Parallel Knowledge Discovery System for Amino Acid Sequences (1995)

T. Shoudai, M. Lappe, S. Miyano, A. Shinohara, T. Okazaki, S. Arikawa, ...

We have developed a machine discovery system BONSAI which receives positive and negative examples as inputs and produces as a hypothesis a pair of a decision tree over regular patterns and an...

On validity of perturbative quantization of the breathing mode in the Skyrme model (1993)

Kostyuk, A. P., Kobushkin, A. P., Chepilko, N. M., Okazaki, T.

We present a detailed discussion of the breathing mode quantization in the Skyrme model and demonstrate that the chiral angle of the hedgehog soliton is strongly affected by the breathing motion.

Quantum solitons of the nonlinear sigma-model with broken chiral symmetry (1993)

Kostyuk, A. P., Kobushkin, A. P., Chepilko, N. M., Okazaki, T.

It is proved that the quantum-mechanical consideration of global breathing of a hedgehog-like field configuration leads to the dynamically stable soliton solutions in the nonlinear sigma-model...

Nucleotide sequence of the human tRNAUUGGln gene (1991)

Nemoto, F., Okazaki, T., Mizushima, H., Müller, W.E.G., Kuchino, Y.

Due to a typographical error, a species of glutamine tRNA described in the above paper was represented incorrectly.The two species of glutamine tRNA discussed should read as follows: $${\hbox{ tRNA...

Identification of a predominant replication origin in fission yeast.

Okuno, Y, Okazaki, T, Masukata, H

We have identified five autonomously replicating sequences (ARSs) in a 100 kbp region of the Schizosaccharomyces pombe chromosome II. Analyses of replicative intermediates of the chromosome DNA by...

Interaction of transcription factor YY1 with a replication-enhancing element, REE1, in an autonomously replicating human chromosome fragment.

Obuse, C, Okazaki, T, Masukata, H

We have previously shown that autonomous replication of human chromosome fragments is stimulated by the presence of an 18 bp sequence, REE1, which exhibits transcriptional silencer activity. The REE1...

Molecular cloning of rat obese cDNA and augmented gene expression in genetically obese Zucker fatty (fa/fa) rats.

Ogawa, Y, Masuzaki, H, Isse, N, Okazaki, T, Mori, K, Shigemoto, M, ...

The obese (ob) gene has recently been isolated through a positional cloning approach, the mutation of which causes a marked hereditary obesity and diabetes mellitus in mice. In the present study, we...

Cell cycle-dependent transcription from the gid and mioC promoters of Escherichia coli.

Ogawa, T, Okazaki, T

Transcription from the gid and mioC promoters, which neighbor the origin of replication of the Escherichia coli chromosome (oriC), has been implicated in the control of initiation of replication of...

DNA replication in Escherichia coli mutants that lack protein HU.

Ogawa, T, Wada, M, Kano, Y, Imamoto, F, Okazaki, T

DNA replication in Escherichia coli cells lacking protein HU was studied. HU has been suggested to be involved in the initiation of replication from in vitro studies. The isolated HU mutants,...

Isolation and sequencing of a genomic clone encoding aspartic proteinase of Rhizopus niveus.

Horiuchi, H, Yanai, K, Okazaki, T, Takagi, M, Yano, K

A gene encoding Rhizopus niveus aspartic proteinase was isolated from an R. niveus genomic library by using oligonucleotides probes corresponding to its partial amino acid sequence, and its...

Analysis of protein-DNA and protein-protein interactions of centromere protein B (CENP-B) and properties of the DNA-CENP-B complex in the cell cycle.

Kitagawa, K, Masumoto, H, Ikeda, M, Okazaki, T

We previously reported that centromere protein B (CENP-B) forms a stable complex (designated complex A) containing two alphoid DNAs in vitro. Domains in the CENP-B polypeptide involved in the...

Centromere protein B of African green monkey cells: gene structure, cellular expression, and centromeric localization.

Yoda, K, Nakamura, T, Masumoto, H, Suzuki, N, Kitagawa, K, Nakano, M, ...

Centromere protein B (CENP-B) is a centromeric DNA-binding protein which recognizes a 17-bp sequence (CENP-B box) in human and mouse centromeric satellite DNA. The African green monkey (AGM) is...

RBP-L, a transcription factor related to RBP-Jkappa.

Minoguchi, S, Taniguchi, Y, Kato, H, Okazaki, T, Strobl, L J, Zimber-Strobl, U, ...

RBP-Jkappa is a sequence-specific DNA binding protein which plays a central role in signalling downstream of the Notch receptor by physically interacting with its intracellular region. Although at...

Autonomous replication of human chromosomal DNA fragments in human cells.

Masukata, H, Satoh, H, Obuse, C, Okazaki, T

We have examined whether a human chromosome has distinct segments that can replicate autonomously as extrachromosomal elements. Human 293S cells were transfected with a set of human chromosomal DNA...

A replication-enhancing element with transcriptional silencer activity in autonomously replicating human chromosomal fragments.

Obuse, C, Okuno, Y, Okazaki, T, Masukata, H

We have identified specific nucleotide sequences involved in autonomous replication of human chromosomal fragments in human cells. Nested deletion analysis of a 10.2-kb long human chromosomal...

Relative roles of T7 RNA polymerase and gene 4 primase for the initiation of T7 phage DNA replication in vivo.

Sugimoto, K, Kohara, Y, Okazaki, T

Initiation sites of T7 phage DNA replication in the presence and absence of T7 phage gene 4 primase have been analyzed by using Escherichia coli cells infected with T7 phage amber mutants, T73,6 and...

Initiation sites for discontinuous DNA synthesis of bacteriophage T7.

Fujiyama, A, Kohara, Y, Okazaki, T

We have previously shown that the discontinuous replication of bacteriophage T7 DNA is primed by tetraribonucleotides (major component) or pentaribonucleotides. Both tetramers and pentamers start...

Alkali-labile structures linked to the 5' ends of Bacillus subtilis short DNA chains.

Yasuda, H, Okazaki, T

Alkali-labile portion covalently linked to the 5' ends of Bacillus subtilis short DNA chains, the putative primer RNA for discontinuous DNA synthesis, was isolated and analyzed using a temperature...

The distribution and properties of RNA primed initiation sites of DNA synthesis at the replication origin of Escherichia coli chromosome.

Kohara, Y, Tohdoh, N, Jiang, X W, Okazaki, T

RNA-linked DNA molecules were obtained from E. coli dnaCts cells synchronously initiating a new round of chromosome replication. The deoxynucleotides at the transition from primer RNA to DNA were...

Primer RNA for DNA synthesis on single-stranded DNA template in a cell free system from Drosophila melanogaster embryos.

Yoda, K, Okazaki, T

A cytoplasmic extract of Drosophila melanogaster early embryos supported DNA synthesis which was dependent on an added single stranded DNA template, phi X174 viral DNA. The product DNA made during...

Characterization of nascent DNA fragments produced by excision of uracil residues in DNA.

Machida, Y, Okazaki, T, Miyake, T, Ohtsuka, E, Ikehara, M

Nascent short DNA chains could result from repair of incorporated uracil residues or be intermediates in discontinuous replication. We have characterized short DNA chains having...

RNA-primed initiation sites of DNA replication in the origin region of bacteriophage lambda genome.

Yoda, K, Yasuda, H, Jiang, X W, Okazaki, T

Using DNA molecules synthesized in the early stage of lambda phage infection, deoxynucleotides at the transition sites from primer RNA to DNA synthesis have been mapped in the 1.5 kbase area of the...

RNA-linked nascent DNA pieces in phage T7-infected Escherchia coli. II. Primary structure of the RNA portion.

Seki, T, Okazaki, T

Short DNA chains were purified from phage T7 infected E. coli cells and 5' ends were labeled with 32P. By an alkali-treatment, pNp's rich in pAp and pCp were liberated from the T7 short DNA chains....

T7 gene 6 exonuclease has an RNase H activity.

Shinozaki, K, Okazaki, T

T7 gene 6 exonuclease has been shown to have an RNase H activity as well as a double-strand specific DNase activity by the following experiments: The RNase H activity coelutes with the DNase activity...

Escherichia coli mutants suppressing replication-defective mutations of the ColE1 plasmid.

Naito, S, Kitani, T, Ogawa, T, Okazaki, T, Uchida, H

Mutants of Escherichia coli K-12 have been isolated that suppress cer mutants, ColE1 mutants that are unable to replicate as the plasmid. These host suppressors were designated her, for host factor...

Cell-specific expression of the human parathyroid hormone gene in rat pituitary cells.

Igarashi, T, Okazaki, T, Potter, H, Gaz, R, Kronenberg, H M

The promoter region of the human parathyroid hormone gene was fused to the Escherichia coli neo gene and introduced into GH4C1 rat pituitary and human HeLa cells. Both TATA boxes of the human...

Discontinuous DNA replication of Drosophila melanogaster is primed by octaribonucleotide primer.

Kitani, T, Yoda, K, Okazaki, T

To investigate the precise structure of eucaryotic primer RNA made in vivo, short DNA chains isolated from nuclei of Drosophila melanogaster embryos were analyzed. Post-labeling of 5' ends of short...

Discontinuous replication of replicative form DNA from bacteriophage phiX174.

Machida, Y, Okazaki, T, Okazaki, R

Bacteriophage phiX174 DNA has been labeled with short pulses of [3H]thymidine during synthesis of replicative form molecules in infected Escherichia coli HF4704 cells. The replicating phiX174 DNA was...

Conserved mechanism of negative gene regulation by extracellular calcium. Parathyroid hormone gene versus atrial natriuretic polypeptide gene.

Okazaki, T, Ando, K, Igarashi, T, Ogata, E, Fujita, T

We found that a negative calcium-responsive element (nCaRE) originally reported in the human parathyroid hormone gene is conserved among several genes. The results of the present study show that...

An initiation site of DNA replication with transcriptional enhancer activity present upstream of the c-myc gene.

Iguchi-Ariga, S M, Okazaki, T, Itani, T, Ogata, M, Sato, Y, Ariga, H

We have previously reported that c-myc protein may promote cellular DNA replication by binding to initiation sites of replication. Here we report that a putative origin of human cellular DNA...

A helix-turn-helix structure unit in human centromere protein B (CENP-B).

Iwahara, J, Kigawa, T, Kitagawa, K, Masumoto, H, Okazaki, T, Yokoyama, S

CENP-B has been suggested to organize arrays of centromere satellite DNA into a higher order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes. The...

Fatty-acyl-CoA thioesters inhibit recruitment of steroid receptor co-activator 1 to alpha and gamma isoforms of peroxisome-proliferator-activated receptors by competing with agonists.

Murakami, K, Ide, T, Nakazawa, T, Okazaki, T, Mochizuki, T, Kadowaki, T

Peroxisome-proliferator-activated receptors (PPARs) alpha and gamma are ligand-dependent transcription factors that are key regulators of lipid and carbohydrate homoeostasis. Fatty acids bind to the...

Characterization of zebrafish caspase-3 and induction of apoptosis through ceramide generation in fish fathead minnow tailbud cells and zebrafish embryo.

Yabu, T, Kishi, S, Okazaki, T, Yamashita, M

Caspase-3 was cloned from zebrafish embryos and its properties were characterized to identify the biological implications of caspase in embryogenesis and apoptosis in zebrafish, which is a model...

Identification of a predominant replication origin in fission yeast.

Okuno, Y, Okazaki, T, Masukata, H

We have identified five autonomously replicating sequences (ARSs) in a 100 kbp region of the Schizosaccharomyces pombe chromosome II. Analyses of replicative intermediates of the chromosome DNA by...

Interaction of transcription factor YY1 with a replication-enhancing element, REE1, in an autonomously replicating human chromosome fragment.

Obuse, C, Okazaki, T, Masukata, H

We have previously shown that autonomous replication of human chromosome fragments is stimulated by the presence of an 18 bp sequence, REE1, which exhibits transcriptional silencer activity. The REE1...

Molecular cloning of rat obese cDNA and augmented gene expression in genetically obese Zucker fatty (fa/fa) rats.

Ogawa, Y, Masuzaki, H, Isse, N, Okazaki, T, Mori, K, Shigemoto, M, ...

The obese (ob) gene has recently been isolated through a positional cloning approach, the mutation of which causes a marked hereditary obesity and diabetes mellitus in mice. In the present study, we...

Cell cycle-dependent transcription from the gid and mioC promoters of Escherichia coli.

Ogawa, T, Okazaki, T

Transcription from the gid and mioC promoters, which neighbor the origin of replication of the Escherichia coli chromosome (oriC), has been implicated in the control of initiation of replication of...

DNA replication in Escherichia coli mutants that lack protein HU.

Ogawa, T, Wada, M, Kano, Y, Imamoto, F, Okazaki, T

DNA replication in Escherichia coli cells lacking protein HU was studied. HU has been suggested to be involved in the initiation of replication from in vitro studies. The isolated HU mutants,...

Isolation and sequencing of a genomic clone encoding aspartic proteinase of Rhizopus niveus.

Horiuchi, H, Yanai, K, Okazaki, T, Takagi, M, Yano, K

A gene encoding Rhizopus niveus aspartic proteinase was isolated from an R. niveus genomic library by using oligonucleotides probes corresponding to its partial amino acid sequence, and its...

Analysis of protein-DNA and protein-protein interactions of centromere protein B (CENP-B) and properties of the DNA-CENP-B complex in the cell cycle.

Kitagawa, K, Masumoto, H, Ikeda, M, Okazaki, T

We previously reported that centromere protein B (CENP-B) forms a stable complex (designated complex A) containing two alphoid DNAs in vitro. Domains in the CENP-B polypeptide involved in the...

Centromere protein B of African green monkey cells: gene structure, cellular expression, and centromeric localization.

Yoda, K, Nakamura, T, Masumoto, H, Suzuki, N, Kitagawa, K, Nakano, M, ...

Centromere protein B (CENP-B) is a centromeric DNA-binding protein which recognizes a 17-bp sequence (CENP-B box) in human and mouse centromeric satellite DNA. The African green monkey (AGM) is...

RBP-L, a transcription factor related to RBP-Jkappa.

Minoguchi, S, Taniguchi, Y, Kato, H, Okazaki, T, Strobl, L J, Zimber-Strobl, U, ...

RBP-Jkappa is a sequence-specific DNA binding protein which plays a central role in signalling downstream of the Notch receptor by physically interacting with its intracellular region. Although at...

Autonomous replication of human chromosomal DNA fragments in human cells.

Masukata, H, Satoh, H, Obuse, C, Okazaki, T

We have examined whether a human chromosome has distinct segments that can replicate autonomously as extrachromosomal elements. Human 293S cells were transfected with a set of human chromosomal DNA...

A replication-enhancing element with transcriptional silencer activity in autonomously replicating human chromosomal fragments.

Obuse, C, Okuno, Y, Okazaki, T, Masukata, H

We have identified specific nucleotide sequences involved in autonomous replication of human chromosomal fragments in human cells. Nested deletion analysis of a 10.2-kb long human chromosomal...

Relative roles of T7 RNA polymerase and gene 4 primase for the initiation of T7 phage DNA replication in vivo.

Sugimoto, K, Kohara, Y, Okazaki, T

Initiation sites of T7 phage DNA replication in the presence and absence of T7 phage gene 4 primase have been analyzed by using Escherichia coli cells infected with T7 phage amber mutants, T73,6 and...

Initiation sites for discontinuous DNA synthesis of bacteriophage T7.

Fujiyama, A, Kohara, Y, Okazaki, T

We have previously shown that the discontinuous replication of bacteriophage T7 DNA is primed by tetraribonucleotides (major component) or pentaribonucleotides. Both tetramers and pentamers start...

Alkali-labile structures linked to the 5' ends of Bacillus subtilis short DNA chains.

Yasuda, H, Okazaki, T

Alkali-labile portion covalently linked to the 5' ends of Bacillus subtilis short DNA chains, the putative primer RNA for discontinuous DNA synthesis, was isolated and analyzed using a temperature...

The distribution and properties of RNA primed initiation sites of DNA synthesis at the replication origin of Escherichia coli chromosome.

Kohara, Y, Tohdoh, N, Jiang, X W, Okazaki, T

RNA-linked DNA molecules were obtained from E. coli dnaCts cells synchronously initiating a new round of chromosome replication. The deoxynucleotides at the transition from primer RNA to DNA were...

Primer RNA for DNA synthesis on single-stranded DNA template in a cell free system from Drosophila melanogaster embryos.

Yoda, K, Okazaki, T

A cytoplasmic extract of Drosophila melanogaster early embryos supported DNA synthesis which was dependent on an added single stranded DNA template, phi X174 viral DNA. The product DNA made during...

Characterization of nascent DNA fragments produced by excision of uracil residues in DNA.

Machida, Y, Okazaki, T, Miyake, T, Ohtsuka, E, Ikehara, M

Nascent short DNA chains could result from repair of incorporated uracil residues or be intermediates in discontinuous replication. We have characterized short DNA chains having...

RNA-primed initiation sites of DNA replication in the origin region of bacteriophage lambda genome.

Yoda, K, Yasuda, H, Jiang, X W, Okazaki, T

Using DNA molecules synthesized in the early stage of lambda phage infection, deoxynucleotides at the transition sites from primer RNA to DNA synthesis have been mapped in the 1.5 kbase area of the...

RNA-linked nascent DNA pieces in phage T7-infected Escherchia coli. II. Primary structure of the RNA portion.

Seki, T, Okazaki, T

Short DNA chains were purified from phage T7 infected E. coli cells and 5' ends were labeled with 32P. By an alkali-treatment, pNp's rich in pAp and pCp were liberated from the T7 short DNA chains....

T7 gene 6 exonuclease has an RNase H activity.

Shinozaki, K, Okazaki, T

T7 gene 6 exonuclease has been shown to have an RNase H activity as well as a double-strand specific DNase activity by the following experiments: The RNase H activity coelutes with the DNase activity...

Escherichia coli mutants suppressing replication-defective mutations of the ColE1 plasmid.

Naito, S, Kitani, T, Ogawa, T, Okazaki, T, Uchida, H

Mutants of Escherichia coli K-12 have been isolated that suppress cer mutants, ColE1 mutants that are unable to replicate as the plasmid. These host suppressors were designated her, for host factor...

Cell-specific expression of the human parathyroid hormone gene in rat pituitary cells.

Igarashi, T, Okazaki, T, Potter, H, Gaz, R, Kronenberg, H M

The promoter region of the human parathyroid hormone gene was fused to the Escherichia coli neo gene and introduced into GH4C1 rat pituitary and human HeLa cells. Both TATA boxes of the human...

Discontinuous DNA replication of Drosophila melanogaster is primed by octaribonucleotide primer.

Kitani, T, Yoda, K, Okazaki, T

To investigate the precise structure of eucaryotic primer RNA made in vivo, short DNA chains isolated from nuclei of Drosophila melanogaster embryos were analyzed. Post-labeling of 5' ends of short...

Discontinuous replication of replicative form DNA from bacteriophage phiX174.

Machida, Y, Okazaki, T, Okazaki, R

Bacteriophage phiX174 DNA has been labeled with short pulses of [3H]thymidine during synthesis of replicative form molecules in infected Escherichia coli HF4704 cells. The replicating phiX174 DNA was...

Conserved mechanism of negative gene regulation by extracellular calcium. Parathyroid hormone gene versus atrial natriuretic polypeptide gene.

Okazaki, T, Ando, K, Igarashi, T, Ogata, E, Fujita, T

We found that a negative calcium-responsive element (nCaRE) originally reported in the human parathyroid hormone gene is conserved among several genes. The results of the present study show that...

An initiation site of DNA replication with transcriptional enhancer activity present upstream of the c-myc gene.

Iguchi-Ariga, S M, Okazaki, T, Itani, T, Ogata, M, Sato, Y, Ariga, H

We have previously reported that c-myc protein may promote cellular DNA replication by binding to initiation sites of replication. Here we report that a putative origin of human cellular DNA...

A helix-turn-helix structure unit in human centromere protein B (CENP-B).

Iwahara, J, Kigawa, T, Kitagawa, K, Masumoto, H, Okazaki, T, Yokoyama, S

CENP-B has been suggested to organize arrays of centromere satellite DNA into a higher order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes. The...

Fatty-acyl-CoA thioesters inhibit recruitment of steroid receptor co-activator 1 to alpha and gamma isoforms of peroxisome-proliferator-activated receptors by competing with agonists.

Murakami, K, Ide, T, Nakazawa, T, Okazaki, T, Mochizuki, T, Kadowaki, T

Peroxisome-proliferator-activated receptors (PPARs) alpha and gamma are ligand-dependent transcription factors that are key regulators of lipid and carbohydrate homoeostasis. Fatty acids bind to the...

Characterization of zebrafish caspase-3 and induction of apoptosis through ceramide generation in fish fathead minnow tailbud cells and zebrafish embryo.

Yabu, T, Kishi, S, Okazaki, T, Yamashita, M

Caspase-3 was cloned from zebrafish embryos and its properties were characterized to identify the biological implications of caspase in embryogenesis and apoptosis in zebrafish, which is a model...

Polyclonal nature of diffuse proliferation of interstitial cells of Cajal in patients with familial and multiple gastrointestinal stromal tumours

Chen, H, Hirota, S, Isozaki, K, Sun, H, Ohashi, A, Kinoshita, K, ...

Background: Diffuse proliferation of interstitial cells of Cajal (ICCs) in the myenteric plexus layer of the intestine has been described in patients with familial and multiple gastrointestinal...

A vascular smooth muscle-specific CD4+ T cell line that induces pulmonary vasculitis in MRL-+/+ mice

SUMITA, S, OZAKI, S, OKAZAKI, T, SOBAJIMA, J, NAKAO, K

We established a T cell line, MV1, specific for rat vascular smooth muscle antigen from the regional lymph nodes of immunized MRL/Mp-+/+ mice. Adoptive transfer of MV1 T cells induced vasculitis...

Anti-neutrophil cytoplasmic antibodies (ANCA) in ulcerative colitis: anti-cathepsin G and a novel antibody correlate with a refractory type

SOBAJIMA, J, OZAKI, S, OKAZAKI, T, OSAKADA, F, SUMITA, S, MORI, K, ...

We analysed the clinical significance of ANCA in patients with ulcerative colitis. On either an indirect immunofluorescence assay or an ELISA with fixed neutrophils, 71% (25/35) of the patients were...

Enhanced expression of vascular endothelial growth factor in lungs of newborn infants with congenital diaphragmatic hernia and pulmonary hypertension

Shehata, S., Mooi, W., Okazaki, T., El-Banna, I., Sharma, H., Tibboel, D.

BACKGROUND—Pulmonary hypoplasia accompanied by pulmonary hypertension resistant to treatment is an important feature of congenital diaphragmatic hernia (CDH). The pathogenesis of the pulmonary...

Evolution of Economic Systems: The Case of Japan

Okazaki, T., Okuno-Fujiwara, M.

In this paper, we shall provide a theoretical overview of what are the chief implications of fucusing institutions and economic systems, what are the main sources of their evolution, how are their...

Clonality analysis of lymphoproliferative disorders in patients with Sjögren's syndrome

Dong, L, Masaki, Y, Takegami, T, Jin, Z-X, Huang, C-R, Fukushima, T, ...

The aim of this study was to clarify the nature of the clonal lymphocyte infiltration in Sjögren's syndrome (SS) patients associated with lymphoproliferative disorders. We examined B cell clonality...