Lampman, Robert W., Sawai, T., Harmon, C. M., Coran, Arnold G., Drongowski, Robert A.
The activity of phospholipase A 2 (PLA 2 ) is elevated in the intestinal epithelia of patients with inflammatory bowel disease (IBD). We recently reported that PLA 2 mediates hydrolysis of...
Drongowski, Robert A., Goldstone, N., Harmon, C. M., Sawai, T., Coran, Arnold G.
Intestinal secretory immunoglobulin A (sIgA) plays an important role in gut mucosal immunity in vivo; however, in-vitro enterocyte models for studying the mechanisms of these effects are lacking....
The effect of phospholipase A2 on bacterial translocation in a cell culture model (2000)
Sawai, T., Drongowski, Robert A., Coran, Arnold G., Usui, N., Harmon, C. M., Dwaihy, J., ...
The activity of phospholipase (PL)A 2 is elevated in the intestinal epithelia of patients with inflammatory bowel disease (IBD). Recently, we reported that lysophosphatidylcholine (L-PC), the PLA...
Three-dimensional imaging of objects in accumulated snow using multifrequency holography (1988)
Sakamoto, Y., Tajiri, K., Sawai, T., Aoki, Y., 坂本, 雄児
The three-dimensional (3-D) imaging of objects buried in snow using multifrequency holography is discussed. It is pointed out that the cross-sectional images obtained by radar used for target...
Three-dimensional imaging of objects in accumulated snow using multifrequency holography (1988)
Sakamoto, Y., Tajiri, K., Sawai, T., Aoki, Y.
The three-dimensional (3-D) imaging of objects buried in snow using multifrequency holography is discussed. It is pointed out that the cross-sectional images obtained by radar used for target...
Three-dimensional imaging of objects in accumulated snow using multifrequency holography (1988)
Sakamoto, Y., Tajiri, K., Sawai, T., Aoki, Y.
The three-dimensional (3-D) imaging of objects buried in snow using multifrequency holography is discussed. It is pointed out that the cross-sectional images obtained by radar used for target...
MÖSSBAUER STUDIES ON SOME π-CYCLOPENTADIENYLIRON CARBONYL COMPLEXES (1974)
Sawai, T., Martin, J., Butler, I., Simkin, D.
The Mössbauer spectra of a number of π-cyclopentadienyliron carbonyl complexes were measured at 96 K. The general formula of the complexes studied is CpFe(CO)2L where L = SMe2, SEt2, SPrn2,...
MÖSSBAUER STUDIES ON SOME π-CYCLOPENTADIENYLIRON CARBONYL COMPLEXES (1974)
Sawai, T., Martin, J., Butler, I., Simkin, D.
The Mössbauer spectra of a number of π-cyclopentadienyliron carbonyl complexes were measured at 96 K. The general formula of the complexes studied is CpFe(CO)2L where L = SMe2, SEt2, SPrn2,...
MÖSSBAUER STUDIES ON SOME π-CYCLOPENTADIENYLIRON CARBONYL COMPLEXES (1974)
Sawai, T., Martin, J., Butler, I., Simkin, D.
The Mössbauer spectra of a number of π-cyclopentadienyliron carbonyl complexes were measured at 96 K. The general formula of the complexes studied is CpFe(CO)2L where L = SMe2, SEt2, SPrn2,...
Someya, Y, Yamaguchi, A, Sawai, T
A novel tetracycline derivative, DMG-DMDOT [9-(N,N-dimethylglycylamido)-6-demethyl-6-deoxytetracycline] , is one of the glycylcyclines which have a broad antibacterial spectrum, including many...
Sawai, T, Tomono, K, Yanagihara, K, Yamamoto, Y, Kaku, M, Hirakata, Y, ...
We describe a novel mouse model of acute staphylococcal pneumonia induced by intravenous injection of Staphylococcus aureus enmeshed in agar beads. For comparison, we also used various strains of...
Mitsuyama, J, Hiruma, R, Yamaguchi, A, Sawai, T
Proteus mirabilis, Proteus vulgaris, Morganella morganii, Providencia rettgeri, and Providencia alcalifaciens, which were once classified into the same genus, Proteus, were studied....
Murayama, S Y, Yamamoto, T, Suzuki, I, Sawai, T
A mutation in a chromosomal gene of Escherichia coli, designated reb, acted in trans to increase the expression of the cloned beta-lactamase gene of Citrobacter freundii. The reb gene was located...
Sawai, T, Hiruma, R, Kawana, N, Kaneko, M, Taniyasu, F, Inami, A
Mutant strains lacking outer membrane protein(s) were isolated from Escherichia coli, Proteus mirabilis, and Enterobacter cloacae. The outer membrane protein(s) of P. mirabilis and E. cloacae...
Kinetic studies on inactivation of Citrobacter freundii cephalosporinase by sulbactam.
Yamaguchi, A, Hirata, T, Sawai, T
The inactivation kinetics for inhibition by sulbactam (CP45,899) of Citrobacter freundii GN346 cephalosporinase were studied in detail and compared with those of type Ib penicillinase or TEM-2...
Kinetic studies on the inhibition of Proteus vulgaris beta-lactamase by imipenem.
Hashizume, T, Yamaguchi, A, Hirata, T, Sawai, T
Imipenem was found to inhibit Proteus vulgaris beta-lactamase in a progressive manner. Kinetic experiments confirmed that the inactivated enzyme was not completely recovered after intact imipenem had...
Novel carbapenem derivative SF2103A: studies on the mode of beta-lactamase inactivation.
Yamaguchi, A, Hirata, T, Sawai, T
A novel carbapenem, SF2103A, is a strong inhibitor of various types of beta-lactamase. Equimolar concentrations of SF2103A completely inactivated the cephalosporinases of Proteus vulgaris and...
Nukaga, M, Tsukamoto, K, Yamaguchi, H, Sawai, T
The class C beta-lactamase of Citrobacter freundii GN346 is a typical cephalosporinase comprising 361 amino acids, and substitution of the glutamic acid at position 219 in the enzyme by lysine was...
Sakurai, Y, Tsukamoto, K, Sawai, T
The structural gene of a carbenicillinase was cloned from the chromosomal DNA of Proteus mirabilis GN79. This gene codes for a protein of 270 amino acids. Alignment of the amino acid sequence with...
Tsukamoto, K, Ohno, R, Sawai, T
The cephalosporinase of Citrobacter freundii GN346 is a class C beta-lactamase, consisting of 361 amino acids and exhibiting the substrate profile of a typical cephalosporinase. On the conversion of...
Kaneko, M, Yamaguchi, A, Sawai, T
Two major outer membrane proteins of Enterobacter cloacae 206 were purified and identified as porins by using reconstituted vesicles. The 37-kilodalton porin forms a channel with a radius of 0.6 nm,...
Characterization of eight beta-lactamases of Gram-negative bacteria.
Sawai, T, Kanno, M, Tsukamoto, K
Eight kinds of beta-lactamases produced by gram-negative bacteria were characterized by the following properties: molecular weight, isoelectric point, pH optimum, molecular activity, immunochemical...
Evolution of complex resistance transposons from an ancestral mercury transposon.
Tanaka, M, Yamamoto, T, Sawai, T
The molecular interrelationship of a transposon family which confers multiple antibiotic resistance and is assumed to have been generated from an ancestral mercury transposon was analyzed. Initially,...
Sawai, T, Takahashi, I, Nakagawa, H, Yamagishi, S
Antiserum against an oxacillin-hydrolyzing penicillinase of Aeromonas hydrophila did not show immunochemical cross-reaction with four oxacillin-hydrolyzing penicillinases mediated by R plasmids.
Delta pH-dependent accumulation of tetracycline in Escherichia coli.
Yamaguchi, A, Ohmori, H, Kaneko-Ohdera, M, Nomura, T, Sawai, T
The effects of ionophores on tetracycline accumulation in Escherichia coli cells were investigated in the presence of polymyxin B nonapeptide. Accumulation was inhibited by nigericin but not by...
Mabuchi, I, Tsukita, S, Tsukita, S, Sawai, T
The cleavage-furrow region was isolated surgically from newt eggs at the early stage of the first cleavage. The isolated furrow contracted in the presence of ATP at a Ca2+ concentration of 10 or 100...
Satoh, J, Seino, H, Abo, T, Tanaka, S, Shintani, S, Ohta, S, ...
We previously reported that administration of a streptococcal preparation (OK-432) inhibited insulitis and development of autoimmune diabetes in nonobese diabetic (NOD) mice and BB rats as animals...
Ueki, Y, Naito, I, Oohashi, T, Sugimoto, M, Seki, T, Yoshioka, H, ...
Diffuse esophageal leiomyomatosis (DL), a benign smooth-muscle-cell tumor, is characterized by abnormal cell proliferation. DL is sometimes associated with X-linked Alport syndrome (AS), an inherited...
Someya, Y, Yamaguchi, A, Sawai, T
A novel tetracycline derivative, DMG-DMDOT [9-(N,N-dimethylglycylamido)-6-demethyl-6-deoxytetracycline] , is one of the glycylcyclines which have a broad antibacterial spectrum, including many...
Sawai, T, Tomono, K, Yanagihara, K, Yamamoto, Y, Kaku, M, Hirakata, Y, ...
We describe a novel mouse model of acute staphylococcal pneumonia induced by intravenous injection of Staphylococcus aureus enmeshed in agar beads. For comparison, we also used various strains of...
Mitsuyama, J, Hiruma, R, Yamaguchi, A, Sawai, T
Proteus mirabilis, Proteus vulgaris, Morganella morganii, Providencia rettgeri, and Providencia alcalifaciens, which were once classified into the same genus, Proteus, were studied....
Murayama, S Y, Yamamoto, T, Suzuki, I, Sawai, T
A mutation in a chromosomal gene of Escherichia coli, designated reb, acted in trans to increase the expression of the cloned beta-lactamase gene of Citrobacter freundii. The reb gene was located...
Sawai, T, Hiruma, R, Kawana, N, Kaneko, M, Taniyasu, F, Inami, A
Mutant strains lacking outer membrane protein(s) were isolated from Escherichia coli, Proteus mirabilis, and Enterobacter cloacae. The outer membrane protein(s) of P. mirabilis and E. cloacae...
Kinetic studies on inactivation of Citrobacter freundii cephalosporinase by sulbactam.
Yamaguchi, A, Hirata, T, Sawai, T
The inactivation kinetics for inhibition by sulbactam (CP45,899) of Citrobacter freundii GN346 cephalosporinase were studied in detail and compared with those of type Ib penicillinase or TEM-2...
Kinetic studies on the inhibition of Proteus vulgaris beta-lactamase by imipenem.
Hashizume, T, Yamaguchi, A, Hirata, T, Sawai, T
Imipenem was found to inhibit Proteus vulgaris beta-lactamase in a progressive manner. Kinetic experiments confirmed that the inactivated enzyme was not completely recovered after intact imipenem had...
Novel carbapenem derivative SF2103A: studies on the mode of beta-lactamase inactivation.
Yamaguchi, A, Hirata, T, Sawai, T
A novel carbapenem, SF2103A, is a strong inhibitor of various types of beta-lactamase. Equimolar concentrations of SF2103A completely inactivated the cephalosporinases of Proteus vulgaris and...
Nukaga, M, Tsukamoto, K, Yamaguchi, H, Sawai, T
The class C beta-lactamase of Citrobacter freundii GN346 is a typical cephalosporinase comprising 361 amino acids, and substitution of the glutamic acid at position 219 in the enzyme by lysine was...
Sakurai, Y, Tsukamoto, K, Sawai, T
The structural gene of a carbenicillinase was cloned from the chromosomal DNA of Proteus mirabilis GN79. This gene codes for a protein of 270 amino acids. Alignment of the amino acid sequence with...
Tsukamoto, K, Ohno, R, Sawai, T
The cephalosporinase of Citrobacter freundii GN346 is a class C beta-lactamase, consisting of 361 amino acids and exhibiting the substrate profile of a typical cephalosporinase. On the conversion of...
Kaneko, M, Yamaguchi, A, Sawai, T
Two major outer membrane proteins of Enterobacter cloacae 206 were purified and identified as porins by using reconstituted vesicles. The 37-kilodalton porin forms a channel with a radius of 0.6 nm,...
Characterization of eight beta-lactamases of Gram-negative bacteria.
Sawai, T, Kanno, M, Tsukamoto, K
Eight kinds of beta-lactamases produced by gram-negative bacteria were characterized by the following properties: molecular weight, isoelectric point, pH optimum, molecular activity, immunochemical...
Evolution of complex resistance transposons from an ancestral mercury transposon.
Tanaka, M, Yamamoto, T, Sawai, T
The molecular interrelationship of a transposon family which confers multiple antibiotic resistance and is assumed to have been generated from an ancestral mercury transposon was analyzed. Initially,...
Sawai, T, Takahashi, I, Nakagawa, H, Yamagishi, S
Antiserum against an oxacillin-hydrolyzing penicillinase of Aeromonas hydrophila did not show immunochemical cross-reaction with four oxacillin-hydrolyzing penicillinases mediated by R plasmids.
Delta pH-dependent accumulation of tetracycline in Escherichia coli.
Yamaguchi, A, Ohmori, H, Kaneko-Ohdera, M, Nomura, T, Sawai, T
The effects of ionophores on tetracycline accumulation in Escherichia coli cells were investigated in the presence of polymyxin B nonapeptide. Accumulation was inhibited by nigericin but not by...
Mabuchi, I, Tsukita, S, Tsukita, S, Sawai, T
The cleavage-furrow region was isolated surgically from newt eggs at the early stage of the first cleavage. The isolated furrow contracted in the presence of ATP at a Ca2+ concentration of 10 or 100...
Satoh, J, Seino, H, Abo, T, Tanaka, S, Shintani, S, Ohta, S, ...
We previously reported that administration of a streptococcal preparation (OK-432) inhibited insulitis and development of autoimmune diabetes in nonobese diabetic (NOD) mice and BB rats as animals...
Ueki, Y, Naito, I, Oohashi, T, Sugimoto, M, Seki, T, Yoshioka, H, ...
Diffuse esophageal leiomyomatosis (DL), a benign smooth-muscle-cell tumor, is characterized by abnormal cell proliferation. DL is sometimes associated with X-linked Alport syndrome (AS), an inherited...
Determination of interstitial collagenase (MMP-1) in patients with rheumatoid arthritis.
Maeda, S, Sawai, T, Uzuki, M, Takahashi, Y, Omoto, H, Seki, M, ...
OBJECTIVES--To investigate whether interstitial collagenase (MMP-1) concentration in synovial fluid can be useful as a marker for disease activity in rheumatoid arthritis (RA), to determine the main...
Kakeya, H, Udono, H, Maesaki, S, Sasaki, E, Kawamura, S, Hossain, M A, ...
Cryptococcus neoformans causes infection in individuals with defective T cell function, such as AIDS, as well as without underlying disease. It has been suggested that humoral as well as cellular...