On the identity of two q-discrete Painlevé equations and their geometrical derivation (2006)
B. Grammaticos, A. Ramani, T. Takenawa
We show that two recently discovered q-discrete Painlevé equations are one and the same system. Moreover we provide a novel derivation of this q-discrete system based on transformations obtained...
Arp2/3 activity is necessary for efficient formation of E-cadherin adhesive contacts (2004)
Verma, S., Shewan, A. M., Scott, J. A., Helwani, F. M., Den Elzen, N. R., Miki, H., ...
Classical cadherin adhesion molecules are fundamental determinants of cell-cell recognition that function in cooperation with the actin cytoskeleton. Productive cadherin-based cell recognition is...
Arp2/3 activity is necessary for efficient formation of E-cadherin adhesive contacts (2004)
Verma, S., Shewan, A. M., Scott, J. A., Helwani, F. M., Den Elzen, N. R., Miki, H., ...
Classical cadherin adhesion molecules are fundamental determinants of cell-cell recognition that function in cooperation with the actin cytoskeleton. Productive cadherin-based cell recognition is...
The space of initial conditions for linearisable mappings (2002)
Takenawa, T., Eguchi, M., Grammaticos, B., Ohta, Y., Ramani, A., Satsuma, J.
We apply the algebraic-geometric techniques developed for the study of mappings which have the singularity confinement property to mappings which are integrable through linearisation. The main...
Moriya, S, Kazlauskas, A, Akimoto, K, Hirai, S, Mizuno, K, Takenawa, T, ...
Protein kinase C (PKC), a major cellular receptor for tumor-promoting phorbol esters and diacylglycerols (DGs), appears to be involved in a variety of cellular functions, although its activation...
Gotoh, N, Tojo, A, Muroya, K, Hashimoto, Y, Hattori, S, Nakamura, S, ...
Epidermal growth factor (EGF) receptor (EGFR) can induce cell growth and transformation in a ligand-dependent manner. To examine whether the autophosphorylation of EGFR correlates with the capacity...
Tanaka, S, Morishita, T, Hashimoto, Y, Hattori, S, Nakamura, S, Shibuya, M, ...
CRK protein, together with GRB2/ASH and Nck proteins, belongs to the adaptor-type Src homology (SH)2-containing molecules, which transduce signals from tyrosine kinases. Here another guanine...
Matuoka, K, Shibata, M, Yamakawa, A, Takenawa, T
The protein ASH (for abundant Src homology), composed of one Src homology region (SH) 2 and two SH3 domains, was cloned by screening human and rat cDNA libraries with an oligonucleotide probe...
Phosphatidylinositol 4,5-bisphosphate phosphatase regulates the rearrangement of actin filaments.
Sakisaka, T, Itoh, T, Miura, K, Takenawa, T
Phosphatidylinositol 4,5-bisphosphate (PIP2) reorganizes actin filaments by modulating the functions of a variety of actin-regulatory proteins. Until now, it was thought that bound PIP2 is hydrolyzed...
Adenovirus E1A proteins stimulate inositol phospholipid metabolism in PC12 cells.
Shiroki, K, Yamakawa, A, Shibata, M, Takenawa, T, Sugano, S, Nomoto, A
To study the influence of nuclear oncogenes on inositol phospholipid metabolism, we examined the various parameters of inositol phospholipid metabolism in PC12 cells expressing adenovirus type 12 or...
Antibody to phosphatidylinositol 4,5-bisphosphate inhibits oncogene-induced mitogenesis.
Fukami, K, Matsuoka, K, Nakanishi, O, Yamakawa, A, Kawai, S, Takenawa, T
The hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) has been shown to be enhanced in cells transformed by some types of oncogenes and tumor viruses, but it is still unknown whether the...
Ando, A, Yonezawa, K, Gout, I, Nakata, T, Ueda, H, Hara, K, ...
Insulin drives the formation of a complex between tyrosine-phosphorylated IRS-1 and SH2-containing proteins. The SH2-containing protein Grb2 also possesses adjacent SH3 domains, which bind the Ras...
Matuoka, K, Shibasaki, F, Shibata, M, Takenawa, T
The Src homology (SH) region 2 binds to phosphorylated tyrosine residues and SH3 domains may interact with cytoskeletal molecules and GTPase-activating proteins for Rho/Rac proteins (the small...
Miki, H, Miura, K, Takenawa, T
Here we identify a 65 kDa protein (N-WASP) from brain that binds the SH3 domains of Ash/Grb2. The sequence is homologous to Wiskott-Aldrich syndrome protein (WASP). N-WASP has several functional...
Hayashi, H, Miyake, N, Kanai, F, Shibasaki, F, Takenawa, T, Ebina, Y
Insulin causes a dramatic and rapid increase in phosphatidylinositol 3-kinase activity in the anti-phosphotyrosine immunoprecipitates of cells overexpressing the human insulin receptor. This enzyme...
Homma, Y, Emori, Y, Shibasaki, F, Suzuki, K, Takenawa, T
A novel bovine spleen phosphoinositide-specific phospholipase C (PLC) has been identified with respect to immunoreactivity with four independent antibodies against each of the PLC isoenzymes, and...
Different properties of monomer and heterodimer forms of phosphatidylinositol 3-kinases.
Shibasaki, F, Fukui, Y, Takenawa, T
Phosphatidylinositol (PI) 3-kinase plays an important role in the signalling of cell growth. We previously purified two types of PI 3-kinase from bovine thymus, a monomer from (PI 3-kinase I) and a...
Homma, Y, Sakamoto, H, Tsunoda, M, Aoki, M, Takenawa, T, Ooyama, T
In order to examine the mechanisms underlying smooth-muscle cell proliferation, we investigated effect of platelet-derived growth factor (PDGF) dimers on proliferation of rabbit vascular...
Nakanishi, O, Homma, Y, Kawasaki, H, Emori, Y, Suzuki, K, Takenawa, T
Two kinds of phosphoinositide-specific phospholipase C (PLC) were purified from rat liver by acid precipitation and several steps of column chromatography. About 50% of the activity could be...
Phosphatidylinositol 3-kinase binds to alpha-actinin through the p85 subunit.
Shibasaki, F, Fukami, K, Fukui, Y, Takenawa, T
Phosphatidylinositol 3-kinase (PI 3-kinase) has been shown to play an important role in the signal transduction of cell growth. It is also suggested that it is involved in cytoskeletal...
Homma, Y, Hashimoto, T, Nagai, Y, Takenawa, T
Alterations of phospholipid and arachidonic acid metabolism were studied by treatment of guinea-pig peritoneal-exudate macrophages with chemotactic peptide, formylmethionyl-leucylphenylalanine...
Ca2+-dependent and Ca2+-independent degradation of phosphatidylinositol in rabbit vas deferens.
Egawa, K, Sacktor, B, Takenawa, T
The effects of Ca2+ and acetylcholine on the degradation and synthesis of phosphatidylinositol in rabbit vas deferens was studied in vitro by a pulse--chase technique and by measuring the content of...
Suzuki, T, Miki, H, Takenawa, T, Sasakawa, C
Shigella, the causative agent of bacillary dysentery, is capable of directing its own movement in the cytoplasm of infected epithelial cells. The bacterial surface protein VirG recruits host...
The essential role of profilin in the assembly of actin for microspike formation.
Suetsugu, S, Miki, H, Takenawa, T
Profilin was first identified as an actin monomer binding protein; however, recent reports indicate its involvement in actin polymerization. To date, there is no direct evidence of a functional role...
WAVE, a novel WASP-family protein involved in actin reorganization induced by Rac.
Miki, H, Suetsugu, S, Takenawa, T
Rac is a Rho-family small GTPase that induces the formation of membrane ruffles. However, it is poorly understood how Rac-induced reorganization of the actin cytoskeleton, which is essential for...
Interaction of Ash/Grb-2 via its SH3 domains with neuron-specific p150 and p65.
Miura, K, Miki, H, Shimazaki, K, Kawai, N, Takenawa, T
We found that 180 kDa, 150 kDa (p150), 110 kDa, 100 kDa and 65 kDa (p65) proteins comprise the major Ash/Grb-2-binding proteins in bovine brain. Among these proteins, 180 kDa and 100 kDa proteins...
Purification and characterization of a lysophosphatidic acid-specific phosphatase.
Lysophosphatidic acid (LPA)-specific phosphatase was purified 3300-fold from bovine brain cytosol. The purification was achieved by (NH4)2SO4 fractionation and several chromatography steps, such as...
Moriya, S, Kazlauskas, A, Akimoto, K, Hirai, S, Mizuno, K, Takenawa, T, ...
Protein kinase C (PKC), a major cellular receptor for tumor-promoting phorbol esters and diacylglycerols (DGs), appears to be involved in a variety of cellular functions, although its activation...
Gotoh, N, Tojo, A, Muroya, K, Hashimoto, Y, Hattori, S, Nakamura, S, ...
Epidermal growth factor (EGF) receptor (EGFR) can induce cell growth and transformation in a ligand-dependent manner. To examine whether the autophosphorylation of EGFR correlates with the capacity...
Tanaka, S, Morishita, T, Hashimoto, Y, Hattori, S, Nakamura, S, Shibuya, M, ...
CRK protein, together with GRB2/ASH and Nck proteins, belongs to the adaptor-type Src homology (SH)2-containing molecules, which transduce signals from tyrosine kinases. Here another guanine...
Matuoka, K, Shibata, M, Yamakawa, A, Takenawa, T
The protein ASH (for abundant Src homology), composed of one Src homology region (SH) 2 and two SH3 domains, was cloned by screening human and rat cDNA libraries with an oligonucleotide probe...
Phosphatidylinositol 4,5-bisphosphate phosphatase regulates the rearrangement of actin filaments.
Sakisaka, T, Itoh, T, Miura, K, Takenawa, T
Phosphatidylinositol 4,5-bisphosphate (PIP2) reorganizes actin filaments by modulating the functions of a variety of actin-regulatory proteins. Until now, it was thought that bound PIP2 is hydrolyzed...
Adenovirus E1A proteins stimulate inositol phospholipid metabolism in PC12 cells.
Shiroki, K, Yamakawa, A, Shibata, M, Takenawa, T, Sugano, S, Nomoto, A
To study the influence of nuclear oncogenes on inositol phospholipid metabolism, we examined the various parameters of inositol phospholipid metabolism in PC12 cells expressing adenovirus type 12 or...
Antibody to phosphatidylinositol 4,5-bisphosphate inhibits oncogene-induced mitogenesis.
Fukami, K, Matsuoka, K, Nakanishi, O, Yamakawa, A, Kawai, S, Takenawa, T
The hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) has been shown to be enhanced in cells transformed by some types of oncogenes and tumor viruses, but it is still unknown whether the...
Ando, A, Yonezawa, K, Gout, I, Nakata, T, Ueda, H, Hara, K, ...
Insulin drives the formation of a complex between tyrosine-phosphorylated IRS-1 and SH2-containing proteins. The SH2-containing protein Grb2 also possesses adjacent SH3 domains, which bind the Ras...
Matuoka, K, Shibasaki, F, Shibata, M, Takenawa, T
The Src homology (SH) region 2 binds to phosphorylated tyrosine residues and SH3 domains may interact with cytoskeletal molecules and GTPase-activating proteins for Rho/Rac proteins (the small...
Miki, H, Miura, K, Takenawa, T
Here we identify a 65 kDa protein (N-WASP) from brain that binds the SH3 domains of Ash/Grb2. The sequence is homologous to Wiskott-Aldrich syndrome protein (WASP). N-WASP has several functional...
Hayashi, H, Miyake, N, Kanai, F, Shibasaki, F, Takenawa, T, Ebina, Y
Insulin causes a dramatic and rapid increase in phosphatidylinositol 3-kinase activity in the anti-phosphotyrosine immunoprecipitates of cells overexpressing the human insulin receptor. This enzyme...
Homma, Y, Emori, Y, Shibasaki, F, Suzuki, K, Takenawa, T
A novel bovine spleen phosphoinositide-specific phospholipase C (PLC) has been identified with respect to immunoreactivity with four independent antibodies against each of the PLC isoenzymes, and...
Different properties of monomer and heterodimer forms of phosphatidylinositol 3-kinases.
Shibasaki, F, Fukui, Y, Takenawa, T
Phosphatidylinositol (PI) 3-kinase plays an important role in the signalling of cell growth. We previously purified two types of PI 3-kinase from bovine thymus, a monomer from (PI 3-kinase I) and a...
Homma, Y, Sakamoto, H, Tsunoda, M, Aoki, M, Takenawa, T, Ooyama, T
In order to examine the mechanisms underlying smooth-muscle cell proliferation, we investigated effect of platelet-derived growth factor (PDGF) dimers on proliferation of rabbit vascular...
Nakanishi, O, Homma, Y, Kawasaki, H, Emori, Y, Suzuki, K, Takenawa, T
Two kinds of phosphoinositide-specific phospholipase C (PLC) were purified from rat liver by acid precipitation and several steps of column chromatography. About 50% of the activity could be...
Phosphatidylinositol 3-kinase binds to alpha-actinin through the p85 subunit.
Shibasaki, F, Fukami, K, Fukui, Y, Takenawa, T
Phosphatidylinositol 3-kinase (PI 3-kinase) has been shown to play an important role in the signal transduction of cell growth. It is also suggested that it is involved in cytoskeletal...
Homma, Y, Hashimoto, T, Nagai, Y, Takenawa, T
Alterations of phospholipid and arachidonic acid metabolism were studied by treatment of guinea-pig peritoneal-exudate macrophages with chemotactic peptide, formylmethionyl-leucylphenylalanine...
Ca2+-dependent and Ca2+-independent degradation of phosphatidylinositol in rabbit vas deferens.
Egawa, K, Sacktor, B, Takenawa, T
The effects of Ca2+ and acetylcholine on the degradation and synthesis of phosphatidylinositol in rabbit vas deferens was studied in vitro by a pulse--chase technique and by measuring the content of...
Suzuki, T, Miki, H, Takenawa, T, Sasakawa, C
Shigella, the causative agent of bacillary dysentery, is capable of directing its own movement in the cytoplasm of infected epithelial cells. The bacterial surface protein VirG recruits host...
The essential role of profilin in the assembly of actin for microspike formation.
Suetsugu, S, Miki, H, Takenawa, T
Profilin was first identified as an actin monomer binding protein; however, recent reports indicate its involvement in actin polymerization. To date, there is no direct evidence of a functional role...
WAVE, a novel WASP-family protein involved in actin reorganization induced by Rac.
Miki, H, Suetsugu, S, Takenawa, T
Rac is a Rho-family small GTPase that induces the formation of membrane ruffles. However, it is poorly understood how Rac-induced reorganization of the actin cytoskeleton, which is essential for...
Interaction of Ash/Grb-2 via its SH3 domains with neuron-specific p150 and p65.
Miura, K, Miki, H, Shimazaki, K, Kawai, N, Takenawa, T
We found that 180 kDa, 150 kDa (p150), 110 kDa, 100 kDa and 65 kDa (p65) proteins comprise the major Ash/Grb-2-binding proteins in bovine brain. Among these proteins, 180 kDa and 100 kDa proteins...
Purification and characterization of a lysophosphatidic acid-specific phosphatase.
Lysophosphatidic acid (LPA)-specific phosphatase was purified 3300-fold from bovine brain cytosol. The purification was achieved by (NH4)2SO4 fractionation and several chromatography steps, such as...
Aberrant accumulation of phospholipase C-delta in Alzheimer brains.
Shimohama, S., Homma, Y., Suenaga, T., Fujimoto, S., Taniguchi, T., Araki, W., ...
Since phosphoinositide-specific phospholipase C (PLC) is one of the key molecules in signal transduction, the authors assessed its involvement in Alzheimer's disease (AD). Immunostaining of a...