T. Thum

Lox-1 receptor blockade abrogates oxLDL-induced oxidative DNA damage and prevents activation of the transcriptional repressor OCT-1 in human coronary arterial endothelium (2008)

Xenobiotic induced liver injury is a leading cause for drug withdrawal and toxicogenomics may help to identify molecular causes. Here we report studies with cultures of human hepatocytes to detect...

Increase of pro-inflammatory cytokine expression in non-demyelinating early cerebral lesions in nervous canine distemper (2008)

Activation of the lectin-like oxLDL receptor (LOX-1) promotes atherosclerosis. Oxidized LDL (OxLDL) increases production of reactive oxygen species (ROS) and leads to the development of endothelial...

Beineke, A, Markus, S., Borlak, J., Thum, T., Baumgärtner, W.

Canine distemper virus (CDV) infection of the central nervous system results in lesions of the gray and white matter. While a biphasic disease process has been discussed for leukoencephalitis with a...

Assessing seasonality of biochemical CO2 exchange model parameters from micrometeorological flux observations at boreal coniferous forest (2008)

T. Thum, T. Aalto, T. Laurila, M. Aurela, A. Lindroth, T. Vesala

The seasonality of the NEE of the northern boreal coniferous forests was investigated by means of inversion modelling using eddy covariance data. Eddy covariance data was used to optimize the...

Assessing seasonality of boreal coniferous forest CO<sub>2</sub> exchange by estimating biochemical model parameters from micrometeorological flux observations (2008)

T. Thum, T. Aalto, T. Laurila, M. Aurela, A. Lindroth, T. Vesala

The biochemical seasonality of the northern boreal coniferous forests was investigated by means of inversion modelling using eddy covariance data. Eddy covariance data was used to optimize the...

Assessing seasonality of boreal coniferous forest CO2 exchange by estimating biochemical model parameters from micrometeorological flux observations (2008)

T. Thum, T. Aalto, T. Laurila, M. Aurela, A. Lindroth, T. Vesala

The biochemical seasonality of the northern boreal coniferous forests was investigated by means of inversion modelling using eddy covariance data. Eddy covariance data was used to optimize the...

Age-dependent impairment of endothelial progenitor cells is corrected by growth hormone mediated increase of insulin-like growth factor-1 (2007)

Thum, T., Hoeber, S., Froese, S., Klink, I., Stichtenoth, D.O., Galuppo, P., ...

Aging is associated with an increased risk for atherosclerosis. A possible cause is low numbers and dysfunction of endothelial progenitor cells (EPC) which insufficiently repair damaged vascular...

Age-dependent impairment of endothelial progenitor cells is corrected by growth-hormone-mediated increase of insulin-like growth-factor-1 (2007)

Thum, T., Hoeber, S., Klink, I., Stichtenoth, D.O., Tsikas, D., Anker, S.D., ...

Enhanced systemic no bioavailability by growth-hormone mediated IGF-1 increase improves number and function of endothelial progenitor cells in aged individuals (2007)

Thum, T., Hoeber, S., Froese, S., Klink, I., Stichtenoth, D.O., Galuppo, P., ...

MicroRNAs in the human heart: A clue to fetal gene reprogramming in heart failure (2007)

Thum, T., Galuppo, P., Wolf, C., Fiedler, J., Kneitz, S., Laake, L.W. Van, ...

Background - Chronic heart failure is characterized by left ventricular remodeling and reactivation of a fetal gene program; the underlying mechanisms are only partly understood. Here we provide...

Modeling atmospheric CO2 concentration profiles and fluxes above sloping terrain at a boreal site (2006)

T. Aalto, J. Hatakka, U. Karstens, M. Aurela, T. Thum, A. Lohila

CO2 fluxes and concentrations were simulated in the planetary boundary layer above subarctic hilly terrain using a three dimensional model. The model solves the transport equations in the local scale...

Expression of xenobiotic metabolizing enzymes in different lung compartments of smokers and nonsmokers (2006)

Thum, T., Erpenbeck, V.J., Moeller, J., Hohlfeld, J.M., Krug, N., Borlak, J.

Background. Cytochrome P450 monooxygenases (CYP) play an important role in the defense against inhaled toxicants and expression of CYP enzymes may differ amongst various lung cells and tissue...

Modeling atmospheric CO2 concentration profiles and fluxes above sloping terrain at a boreal site (2006)

Aalto, T., Hatakka, J., Karstens, Ute, Aurela, M., Thum, T., Lohila, A.

Age-dependent impairment of endothelial progenitor cells is corrected by growth hormone-mediated increase of insulin-like growth factor-1 (2006)

Thum, T., Hoeber, S., Froese, S., Klink, I., Stichtenoth, D.O., Tsikas, D., ...

Acetylsalicylic acid (aspirin) reduces endothelial cell senescence (2006)

Scalera, F., Borlak, J., Schubert, B., Martens-Lobenhoffer, J., Thum, T., Frolich, J.C., ...

Statins delay endothelial cell senescence (2006)

Scalera, F., Borlak, J., Schubert, B., Martens-Lobenhoffer, J., Thum, T., Frölich, J.C., ...

Age-dependent impairment of endothelial progenitor cells is corrected by growth hormone-mediated increase of insulin-like growth factor-1 (2006)

Thum, T., Hoeber, S., Froese, S., Klink, I., Stichtenoth, D.O., Tsikas, D., ...

Thum, T., Hoeber, S., Froese, S., Ivonne, K., Stichtenoth, D.O., Tsikas, D., ...

Modeling atmospheric CO2 concentration profiles and fluxes above sloping terrain at a boreal site (2006)

Aalto, T., Hatakka, J., Karstens, U., Aurela, M., Thum, T., Lohila, A.

CO2 fluxes and concentrations were simulated in the planetary boundary layer above subarctic hilly terrain using a three dimensional model. The model solves the transport equations in the local scale...

Modeling atmospheric CO2 concentration profiles and fluxes above sloping terrain at a boreal site (2006)

Aalto, T., Hatakka, J., Karstens, U., Aurela, M., Thum, T., Lohila, A.

CO2 fluxes and concentrations were simulated in the planetary boundary layer above subarctic hilly terrain using a three dimensional model. The model solves the transport equations in the local scale...

Clostridium difficile toxin A induces expression of the stress-induced early gene product RhoB (2005)

Gerhard, R., Tatge, H., Genth, H., Thum, T., Borlak, J., Fritz, G., ...

Clostridium difficile toxin A mono-glucosylates the Rho family GTPases Rho, Rac, and Cdc42. Glucosylation leads to functional inactivation of Rho GTPases and causes disruption of the actin...

PCR-Reaktionsgemisch fuer Fluoreszenz-basierende Genexpressions- und Genmutationsanalysen (2005)

Toxicogenomics applied to cardiovascular toxicity (2005)

WO 200222865 A UPAB: 20020618 NOVELTY - Polymerase chain reaction (PCR) mixture (A) for fluorescent-based gene expression and gene mutation analysis comprising: a Taq polymerase (I); bovine serum...

Modeling atmospheric CO2 concentration profiles and fluxes above sloping terrain at a boreal site (2005)

Toxicogenomics is an emerging discipline that uses genomics tools, including gene expression profiling technologies, to address problems of toxicological significance. Initial results are now...

Aalto, T., Hatakka, J., Karstens, U., Aurela, M., Thum, T., Lohila, A.

CO2 fluxes and concentrations were simulated in the planetary boundary layer above subarctic hilly terrain using a three dimensional model. The model solves the transport equations in the local scale...

Modeling atmospheric CO2 concentration profiles and fluxes above sloping terrain at a boreal site (2005)

Aalto, T., Hatakka, J., Karstens, U., Aurela, M., Thum, T., Lohila, A.

CO2 fluxes and concentrations were simulated in the planetary boundary layer above subarctic hilly terrain using a three dimensional model. The model solves the transport equations in the local scale...

Atmospheric particle formation events at Värriö measurement station in Finnish Lapland 1998-2002 (2004)

H. Vehkamäki, M. Dal Maso, T. Hussein, R. Flanagan, A. Hyvärinen, J. Lauros, ...

During the calendar years 1998-2002, 147 clear 8nm diameter particle formation events have been identified at the SMEAR I station in Värriö, northern Finland. The events have been...

Mechanistic role of cytochrome P450 monooxygenases in oxidized low-density lipoprotein-induced vascular injury: Therapy through LOX-1 receptor antagonism? (2004)

Endogenous nitric oxide synthesis inhibitor asymmetric dimethyl L-arginine accelerates endothelial cell senescence (2004)

Oxidized low-density lipoprotein (oxLDL) is an important risk factor for vascular injury. Its role on coronary vasoconstriction remains speculative. Endothelial monooxygenases (cytochrome P450s...

Scalera, F., Borlak, J., Beckmann, B., Martens-Lobenhoffer, J., Thum, T., Tager, M., ...

OBJECTIVE: Asymmetrical dimethylarginine (ADMA) is an endogenous inhibitor of nitric oxide synthase (NOS), and its accumulation has been associated with cardiovascular disease. We aimed to...

Androgen metabolism in thymus of fetal and adult rats (2004)

Borlak, J., Schulte, I., Thum, T.

Cytochrome P450 (P450) monooxygenases play a role in target tissue metabolic activation of xenobiotics and/or endogenous compounds, such as vasoactive molecules or hormones. Indeed, tissue-specific...

Atmospheric particle formation events at Värriö measurement station in Finnish Lapland 1998?2002 (2004)

Vehkamäki, H., Dal Maso, M., Hussein, T., Flanagan, R., Hyvärinen, A., Lauros, J., ...

We have identified 147 clear 8 nm diameter particle formation events at the SMEAR I station in Värriö, northern Finland during calendar years 1998?2002. The events have been classified in detail...

Atmospheric particle formation events at Värriö measurement station in Finnish Lapland 1998-2002 (2004)

Vehkamäki, H., Dal Maso, M., Hussein, T., Flanagan, R., Hyvärinen, A., Lauros, J., ...

During the calendar years 1998-2002, 147 clear 8nm diameter particle formation events have been identified at the SMEAR I station in Värriö, northern Finland. The events have been classified in...

Atmospheric particle formation events at Värriö measurement station in Finnish Lapland 1998?2002 (2004)

Vehkamäki, H., Dal Maso, M., Hussein, T., Flanagan, R., Hyvärinen, A., Lauros, J., ...

We have identified 147 clear 8 nm diameter particle formation events at the SMEAR I station in Värriö, northern Finland during calendar years 1998?2002. The events have been classified in detail...

Atmospheric particle formation events at Värriö measurement station in Finnish Lapland 1998-2002 (2004)

Vehkamäki, H., Dal Maso, M., Hussein, T., Flanagan, R., Hyvärinen, A., Lauros, J., ...

During the calendar years 1998-2002, 147 clear 8nm diameter particle formation events have been identified at the SMEAR I station in Värriö, northern Finland. The events have been classified in...

Atmospheric particle formation events at Värriö measurement station in Finnish Lapland 1998–2002 (2004)

H. Vehkamäki, M. Dal Maso, T. Hussein, R. Flanagan, A. Hyvärinen, J. Lauros, ...

We have identified 147 clear 8 nm diameter particle formation events at the SMEAR I station in Värriö, northern Finland during calendar years 1998–2002. The events have been...

Verapamil: Metabolism in cultures of primary human coronary arterial endothelial cells (2003)

Borlak, J., Walles, M., Levsen, K., Thum, T.

Endothelium is a metabolically active secretory tissue and an important barrier for metabolic products. Little is known about its contribution to drug oxidation. We investigated the gene and protein...

Hallmarks of ion channel gene expression in end-stage heart failure (2003)

Gene expression profiles in the closing ductus and the great vessels (2003)

Electrical conductance is greatly altered in end-stage heart failure, but little is known about the underlying events. We therefore investigated the expression of genes coding for major inward and...

Bökenkamp, R., Thum, T., Borlak, J.

The pharmacological approach to ductal patency is currently focused on prostaglandin metabolism. The role of other vascular factors for ductal remodelling is under discussion. Aim of the present...

Verapamil: Identification of novel metabolites in cultures of primary human hepatocytes and human urine by LC-MS[n] and LC-NMR (2003)

Borlak, J., Walles, M., Elend, M., Thum, T., Preiss, A., Levsen, K.

1. Verapamil is a well-known and world-wide prescribed calcium antagonist, but it suffers from extensive first-pass metabolism. Although it has been marketed for many years, a complete understanding...

Growth hormone induces eNOS expression and nitric oxide release in a cultured human endothelial cell line (2003)

Thum, T., Tsikas, D., Frölich, J.F., Borlak, J.

Growth hormone deficiency is linked to cardiovascular disease and particularly increased peripheral vascular resistance. Surprisingly, its role in endothelial nitric oxide (NO) synthetase (eNOS)...

A rapid and simple CYP2D6 genotyping assay - Case study with the analgetic tramadol (2003)

Borlak, J., Hermann, R., Erb, K., Thum, T.

There is substantial evidence for a causal relationship between genetic variability of the CYP2D6 gene and changes in the pharmacokinetics of drugs. Therefore, knowledge of single-nucleotide...

Metabolism of verapamil: 24 new phase I and phase II metabolites identified in cell cultures of rat hepatocytes by liquid chromatography-tandem mass spectrometry (2003)

OCT-1: A key player in vascular biology - Implications for cardiovascular toxicity : Meeting Abstract (2003)

Verapamil is a widely prescribed calcium antagonist, but suffers from extensive first pass metabolism. Despite its frequent use in drug metabolism a complete understanding of its metabolic pathway is...

Oct-1 protein/DNA interaction - implications for cytochrome- P450 monooxygenase gene-expression: Meeting Abstract (2003)

Oxidized LDL represses cytochrome-P450 monooxygenase catalyzed EDHF production in human coronary arterial endothelium through diminished NF-1 availability (2003)

Aspirin reduces endothelial-cell senescence: Meeting Abstract (2003)

Scalera, F., Thum, T., Borlak, J., Schubert, B., Frolich, J.C., Bode-Boger, S.M.

OCT-1: A key player in vascular biology - Implications for cardiovascular toxicity (2003)

Aroclor 1254 modulates gene expression of nuclear transcription factors: Implications for Albumin gene transcription and protein synthesis in rat hepatocyte cultures (2002)

Borlak, J., Dangers, M., Thum, T.

Human exposure to polychlorinated biphenyls (PCBs) may lead to increased albumin serum levels, but little is known about the underlying events. Certain PCBs are also ligands for the aryl hydrocarbon...

PCBs alter gene expression of nuclear transcription factors and other heart-specific genes in cultures of primary cardiomyocytes: Possible implications for cardiotoxicity (2002)

Testosterone, cytochrome P450, and cardiac hypertrophy (2002)

1. Polychlorinated biphenyls (PCBs) are well-known environmental pollutants that bioaccumulate mainly in the fatty tissue of animals and humans. Although contamination occurs primarily via the food...

Is there a universal housekeeping gene? (2002)

Cytochrome P450 mono-oxygenases (CYP) play an essential role in steroid metabolism, and there is speculation that sex hormones might influence cardiac mass and physiology. As CYP mono-oxygenases...

Lindemann, M., Thum, T., Borlak, J.

The effects of PCB's on the gene expression of transcription factors and other heart specific genes in cultures of primary cardiomyocytes: Possible implications for cardiotoxicity (2002)

oxLDL inhibits eNOS and cytochrome P450 monooxygenases in human arterial coronary endothelial cells (2002)

Derailed steroid metabolism in cardiac hypertrophy (2002)

Verapamil: Identification of novel metabolites in the human heart (2002)

Oxidized LDL modulates cytochrome P450 monooxygease gene expression and protein function in human arterial coronary endothelial cells (2002)

Novel phase I and phase II metabolites of Verapamil identified in cell cultures of rat hepatocytes by LC-MS(n) (2002)

Altered cytochrome P450 monooxygenase activity in heart tissue of spontaneously hypertensive rats (2002)

A new method for identification of major CYP2C19 polymorphisms (2002)

Identification of major CYP2C9 polymorphisms using fluorescence resonance energy transfer (FRET) analysis (2002)

Genotyping of major NAT2 single nucleotide polymorphisms in a German population using fluorescence resonance energy transfer (FRET) analysis (2002)

Identification of major CYP2C9 and CP2C19 polymorphisms by fluorescence resonance energy transfer analysis (2002)

Verapamil. New insight into the molecular mechanism of drug oxidation in the human heart (2002)

Butanedione monoxime increases the viability and yield of adult cardiomyocytes in primary cultures (2001)

Verapamil is a commonly prescribed cardiovascular drug, but surprisingly its metabolism in the target tissue of pharmacotherapy is basically unknown. We therefore investigated its biotransformation...

Verapamil metabolism in rat heart tissue: Abstract for poster presentation (2001)

Various protocols for the isolation and cultivation of adult rat cardiomyocytes were compared, and the cytoprotective potential of the reversible myosin ATPase inhibitor butanedione monoxime (BDM)...

Thum, T., Walles, M., Levsen, K., Borlak, J.

Reprogramming of gene expression in cultures cardiomyocytes by the myosin ATPase inhibitor butanedione monoxime (2001)

Die Hochregulierung S-Adenosyl-L-Methionin-abhängiger Methyltransferasen führt zu einer gesteigerten Bildung von ADMA unter hypercholesterinämischen Bedingungen: Freie Vorträge: Gefäße 7, experimentell Lipoproteine. Vortrag 581 (2001)

BACKGROUND: Butanedione monoxime (BDM) is a reversible myosin ATPase inhibitor. Its use in transplantation medicine may be of benefit in the preservation of hearts. As little is known about its...

Sydow, K., Bode-Böger, S.M., Thum, T., Borlak, J., Münzel, T., Böger, R.H.

Verapamil metabolism in distinct regions of the heart and in cultures of cardiomyocytes of adult rats (2001)

Verapamil metabolism in primary cultures of cardiomyocytes of adult rats and in distinct regions of explanted hearts: Biotransformation (phase 1 and 2) (2001)

A substantial number of drugs act either directly or indirectly on the heart, but surprisingly, little is known about drug oxidation in the heart. We therefore investigated the metabolism of the...

Transcriptional activation of liver enriched transcription factors and the albumin gene in rat hepatocytes by Aroclor 1254: Polyaromatic hydrocarbons and dioxins (TCDD) (2001)

Gene expression and testosterone metabolism in distinct regions of the rat heart: Biotransformation (phase 1 and 2) (2001)

Drug oxidation in the human heart: Biotransformation (phase 1 and 2) (2001)

Cytochrome P450 enzyme activity and mRNA expression in primary rat alveolar epithelial type II cells: Biotransformation (phase 1 and 2) (2001)

Borlak, J., Vata, I., Thum, T., Hansen, T.

Tramadol disposition in CYP2D6 genotyped human subjects. Genetic variations in susceptibility to xenobiotics: Poster 3B10 (2001)

Induction of nuclear transcription factors, cytochrome P450 monooxygenases, and glutathione S-transferase alpha gene expression in Aroclor 1254-treated rat hepatocyte cultures (2001)

Cellular dedifferentiation of endothelium is linked to activation and silencing of certain nuclear transcription factors: Implications for endothelial dysfunction and vascular biology (2001)

Aroclor 1254 is a complex mixture of polychlorinated biphenyls and is well known for its potency to induce drug-metabolising enzymes, but little is known about its ability to modulate gene expression...

Thum, T.

Increased formation of ADMA, an endogenous NOS inhibitor, in hyperchloresterolemia: Upregulation of S-Adenosylmethionine-dependent methyltransferases (2000)

Sydow, K., Bode-Boger, S.M., Thum, T., Borlak, J., Boger, R.H.

Reprogramming of gene expression in cultured cardiomyocytes by the myosin ATPase inhibitor butanedione monoxime (2000)

Cytochrome P450 monooxygenase gene expression and protein activity in cultures of adult cardiomyocytes of the rat (2000)

The myosin ATPase inhibitor BDM modulates ANP gene expression and confers cytoprotection in cultures of adult cardiomyocytes (2000)

Cellular dedifferentiation of endothelium is linked to activation and silencing of certain nuclear transcription factors: Implications for endothelial dysfunction and vascular biology (2000)

Gene expression in distinct regions of the human heart - implications for drug treatment of cardiovascular disease (2000)

Molecular diagnosis of a familial non heamolytic hyperbilirubinemia (Gilbert's Syndrome) in healthy subjects (2000)

Borlak, J., Thum, T., Landt, O., Erb, K., Hermann, R.

Isolation and cultivation of calcium (Ca2+) tolerant cardiomyocytes from the adult rat. Improvements and applications (2000)

Cytochrome P450 monooxygenase gene expression and protein activity in cultures of adult cardiomyocytes of the rat (2000)

1. Primary cultures of cardiomyocytes provide a valuable tool for the study of the pharmacological and toxicological properties of drugs and chemicals, but for several technical reasons...

Cellular dedifferentiation of endothelium is linked to activation and silencing of certain nuclear transcription factors: Implications for endothelial dysfunction and vascular biology (2000)

There are a substantial number of drugs acting either directly or indirectly on the heart, but surprisingly, little is known about the metabolic capacity of heart muscle cells. We therefore...

Thum, T., Haverich, A., Borlak, J.

We investigated the gene expression of the nuclear transcription factors c/EBPalpha, GATA-2, and the silencer Oct-1 in conjunction with the gene expression of all major cytochrome P450 genes and of...

Molecular diagnosis of a familial nonhemolytic hyperbilirubinemia (Gilbert's syndrome) in healthy subjects (2000)

Borlak, J., Thum, T., Landt, O., Erb, K., Hermann, R.

Recent research has shown that congenital nonhemolytic low grade hyperbilirubinemias in patients with Gilbert's syndrome (GS) are linked to mutations in the TATA box upstream of the uridine...

LDL cholesterol upregulates synthesis of asymmetrical dimethylarginine in human endothelial cells: Involvement of S-adenosylmethionine-dependent methyltransferases (2000)

Böger, R.H., Sydow, K., Borlak, J., Thum, T., Lenzen, H., Schubert, B., ...

Asymmetrical dimethylarginine (ADMA) is an endogenous nitric oxide synthase inhibitor. It is formed by protein arginine N-methyltransferases (PRMTs), which utilize S-adenosylmethionine as methyl...

Gene expression in distinct regions of the heart (2000)