U. Riek, R. Scholz, P.V. Konarev, A. Rufer, M. Suter, ...
Journal article
Braissant, O, Henry, H, Villard, A M, Speer, O, Wallimann, T, Bachmann, C
BACKGROUND: Creatine (Cr) is synthesized by a two-step mechanism involving arginine:glycine amidinotransferase (AGAT) and guanidinoacetate methyltransferase (GAMT), and is taken up by cells through a...
Murphy, R., McConell, G., Cameron-Smith, David, Watt, K., Ackland, Leigh, Walzel, B., ...
The present study examined the gene expression and cellular localization of the creatine transporter (CreaT) protein in rat skeletal muscle. Soleus (SOL) and red (RG) and white gastrocnemius (WG)...
Murphy, R., McConell, G., Cameron-Smith, David, Watt, K., Ackland, Leigh, Walzel, B., ...
The present study examined the gene expression and cellular localization of the creatine transporter (CreaT) protein in rat skeletal muscle. Soleus (SOL) and red (RG) and white gastrocnemius (WG)...
Stadhouders, A M, Jap, P H, Winkler, H P, Eppenberger, H M, Wallimann, T
Overaccumulation of abnormally organized mitochondria in so-called "ragged-red" skeletal muscle fibers is a morphological hallmark of mitochondrial myopathies, in particular of mitochondrial...
Wallimann, T, Wegmann, G, Moser, H, Huber, R, Eppenberger, H M
Two isoforms of creatine kinase (CK; ATP:creatine N-phosphotransferase, E.C. 2.7.3.2), brain type (BB-CK) and mitochondrial type (MiMi-CK), but not the muscle types (MM- or hybrid MB-CK), were...
Monovalent antibodies against MM-creatine kinase remove the M line from myofibrils.
Wallimann, T, Pelloni, G, Turner, D C, Eppenberger, H M
Column-purified antibodies against creatine kinase (EC 2.7.3.2) from chicken skeletal muscle (the homodimeric isoenzyme designated MM-CK) bind specifically to the M lines of chicken pectoral muscle...
Barrantes, F J, Mieskes, G, Wallimann, T
The nonreceptor, peripheral v proteins (Mr 43,000 proteins) are conspicuous components of the acetylcholine receptor-rich membranes and the Torpedo electrocyte, so far devoid of any known enzymatic...
Origin of Leg Musculature during Drosophila Metamorphosis
Ursprung, H., Conscience-Egli, M., Fox, D. J., Wallimann, T.
Pro- and mesothoracic leg imaginal disks of late third-instar larvae of genotypes affecting the electrophoretic mobilities of α-glycerolphosphate dehydrogenase (EC 1.1.1.8) and arginine kinase (EC...
Unshadowed myosin molecules: STEM mass-maps of myosin heads.
Walzthöny, D, Bähler, M, Eppenberger, H M, Wallimann, T, Engel, A
Myosin molecules were directly visualized without heavy metal shadowing by scanning transmission electron microscopy (STEM) under low dose conditions. The general appearance and dimensions of heavy...
Expression of active octameric chicken cardiac mitochondrial creatine kinase in Escherichia coli.
Furter, R, Kaldis, P, Furter-Graves, E M, Schnyder, T, Eppenberger, H M, Wallimann, T
Sarcomeric mitochondrial creatine kinase (Mib-CK) of chicken was expressed in Escherichia coli as a soluble enzyme by using an inducible phage-T7 promoter. Up to one third of the protein in E. coli...
Wyss, M, Wallimann, T, Köhrle, J
Besides their well-known regulation of transcription by binding to nuclear receptors, thyroid hormones have been suggested to have direct effects on mitochondria. In a previous study, incubation of...
Wyss, M, Maughan, D, Wallimann, T
Purification and biophysical characterization of mitochondrial creatine kinase (Mi-CK) from sperm of the sea urchin Psammechinus miliaris, as well as gel-permeation chromatography of human heart...
Functional differences between dimeric and octameric mitochondrial creatine kinase.
Mitochondrial creatine kinase (Mi-CK) consists of octameric and dimeric molecules that are interconvertible. In the present study, the kinetic properties of purified chicken heart Mi-CK (Mib-CK)...
Dorsten, F A, Wyss, M, Wallimann, T, Nicolay, K
The kinetics of the creatine kinase (CK) reaction were studied in suspensions of quiescent and active, intact sea-urchin spermatozoa in artificial seawater, using 31P-NMR magnetization transfer. In...
Koufen, P, Rück, A, Brdiczka, D, Wendt, S, Wallimann, T, Stark, G
Free radicals of X-ray-induced water radiolysis, either directly or indirectly via their reaction products, reduce the activity of both dimeric cytoplasmic muscle-type creatine kinase (MM-CK) and...
Kraft, T, Messerli, M, Rothen-Rutishauser, B, Perriard, J C, Wallimann, T, Brenner, B
Confocal laser fluorescence microscopy was used to study in real time under nearly physiological conditions the equilibration and exchange characteristics of several different fluorescently labeled...
Structural changes of creatine kinase upon substrate binding.
Forstner, M, Kriechbaum, M, Laggner, P, Wallimann, T
Small-angle x-ray scattering was used to investigate structural changes upon binding of individual substrates or a transition state analog complex (TSAC; Mg-ADP, creatine, and KNO3) to creatine...
Stadhouders, A M, Jap, P H, Winkler, H P, Eppenberger, H M, Wallimann, T
Overaccumulation of abnormally organized mitochondria in so-called "ragged-red" skeletal muscle fibers is a morphological hallmark of mitochondrial myopathies, in particular of mitochondrial...
Wallimann, T, Wegmann, G, Moser, H, Huber, R, Eppenberger, H M
Two isoforms of creatine kinase (CK; ATP:creatine N-phosphotransferase, E.C. 2.7.3.2), brain type (BB-CK) and mitochondrial type (MiMi-CK), but not the muscle types (MM- or hybrid MB-CK), were...
Monovalent antibodies against MM-creatine kinase remove the M line from myofibrils.
Wallimann, T, Pelloni, G, Turner, D C, Eppenberger, H M
Column-purified antibodies against creatine kinase (EC 2.7.3.2) from chicken skeletal muscle (the homodimeric isoenzyme designated MM-CK) bind specifically to the M lines of chicken pectoral muscle...
Barrantes, F J, Mieskes, G, Wallimann, T
The nonreceptor, peripheral v proteins (Mr 43,000 proteins) are conspicuous components of the acetylcholine receptor-rich membranes and the Torpedo electrocyte, so far devoid of any known enzymatic...
Origin of Leg Musculature during Drosophila Metamorphosis
Ursprung, H., Conscience-Egli, M., Fox, D. J., Wallimann, T.
Pro- and mesothoracic leg imaginal disks of late third-instar larvae of genotypes affecting the electrophoretic mobilities of α-glycerolphosphate dehydrogenase (EC 1.1.1.8) and arginine kinase (EC...
Unshadowed myosin molecules: STEM mass-maps of myosin heads.
Walzthöny, D, Bähler, M, Eppenberger, H M, Wallimann, T, Engel, A
Myosin molecules were directly visualized without heavy metal shadowing by scanning transmission electron microscopy (STEM) under low dose conditions. The general appearance and dimensions of heavy...
Expression of active octameric chicken cardiac mitochondrial creatine kinase in Escherichia coli.
Furter, R, Kaldis, P, Furter-Graves, E M, Schnyder, T, Eppenberger, H M, Wallimann, T
Sarcomeric mitochondrial creatine kinase (Mib-CK) of chicken was expressed in Escherichia coli as a soluble enzyme by using an inducible phage-T7 promoter. Up to one third of the protein in E. coli...
Wyss, M, Wallimann, T, Köhrle, J
Besides their well-known regulation of transcription by binding to nuclear receptors, thyroid hormones have been suggested to have direct effects on mitochondria. In a previous study, incubation of...
Wyss, M, Maughan, D, Wallimann, T
Purification and biophysical characterization of mitochondrial creatine kinase (Mi-CK) from sperm of the sea urchin Psammechinus miliaris, as well as gel-permeation chromatography of human heart...
Functional differences between dimeric and octameric mitochondrial creatine kinase.
Mitochondrial creatine kinase (Mi-CK) consists of octameric and dimeric molecules that are interconvertible. In the present study, the kinetic properties of purified chicken heart Mi-CK (Mib-CK)...
Dorsten, F A, Wyss, M, Wallimann, T, Nicolay, K
The kinetics of the creatine kinase (CK) reaction were studied in suspensions of quiescent and active, intact sea-urchin spermatozoa in artificial seawater, using 31P-NMR magnetization transfer. In...
Koufen, P, Rück, A, Brdiczka, D, Wendt, S, Wallimann, T, Stark, G
Free radicals of X-ray-induced water radiolysis, either directly or indirectly via their reaction products, reduce the activity of both dimeric cytoplasmic muscle-type creatine kinase (MM-CK) and...
Kraft, T, Messerli, M, Rothen-Rutishauser, B, Perriard, J C, Wallimann, T, Brenner, B
Confocal laser fluorescence microscopy was used to study in real time under nearly physiological conditions the equilibration and exchange characteristics of several different fluorescently labeled...
Structural changes of creatine kinase upon substrate binding.
Forstner, M, Kriechbaum, M, Laggner, P, Wallimann, T
Small-angle x-ray scattering was used to investigate structural changes upon binding of individual substrates or a transition state analog complex (TSAC; Mg-ADP, creatine, and KNO3) to creatine...
Gross, M., Furter-Graves, E. M., Wallimann, T., Eppenberger, H. M., Furter, R.
The 5 tryptophan residues of chicken sarcomeric mitochondrial creatine kinase (Mib-CK) were individually replaced by phenylalanine or cysteine using site-directed mutagenesis. The mutant proteins...
Gross, M., Wyss, M., Furter-Graves, E. M., Wallimann, T., Furter, R.
Creatine kinase (CK) has been postulated to consist of two flexibly hinged domains. A previously demonstrated protease-sensitive site in M-CK (Morris & Jackson, 1991) has directed our attempts to...
Forstner, M., Müller, A., Stolz, M., Wallimann, T.
A histidine residue with a pKa of 7 has been inferred to act as a general acid-base catalyst for the reaction of creatine kinase (CK), catalyzing the reversible phosphorylation of creatine by ATP....
Crystal structure of brain-type creatine kinase at 1.41 A resolution.
Eder, M., Schlattner, U., Becker, A., Wallimann, T., Kabsch, W., Fritz-Wolf, K.
Excitable cells and tissues like muscle or brain show a highly fluctuating consumption of ATP, which is efficiently regenerated from a large pool of phosphocreatine by the enzyme creatine kinase...
Peral, M J, García-Delgado, M, Calonge, M L, Durán, J M, De La Horra, M C, Wallimann, T, ...
In spite of all the fascinating properties of oral creatine supplementation, the mechanism(s) mediating its intestinal absorption has(have) not been investigated. The purpose of this study was to...
Brunisholz, R. A., Tuerk, R., Neumann, D., Auchli, Y., Schlapbach, R., Wallimann, T.
Reversible phosphorylation of proteins plays an important role in many cellular processes. To investigate the functions of kinases and their substrates in vivo and in vitro—for example, production...