T. Yokota

Susceptibility of methicillin-resistant Staphylococcus aureus to the selenium-containing compound 2-phenyl-1,2-benzoisoselenazol-3(2H)-one (PZ51).

Plasmid pSAJ1 from a methicillin- and gentamicin-resistant strain of Staphylococcus aureus had am molecular size of 50 kilobases and conferred resistance not only to kanamycin, gentamicin,...

Nozawa, R, Yokota, T, Fujimoto, T

The growth of Staphylococcus aureus 209P was inhibited by 0.20 micrograms of 2-phenyl-1,2-benzoisoselenazol-3(2H)-one (PZ51) per ml, while strains of the family Enterobacteriaceae were more resistant...

Comparative activities of several nucleoside analogs against duck hepatitis B virus in vitro.

Yokota, T, Konno, K, Chonan, E, Mochizuki, S, Kojima, K, Shigeta, S, ...

Duck hepatitis B virus (DHBV) replication in primary duck hepatocytes was monitored by examining the synthesis of both DHBV DNA and DHBV core antigen. Several nucleoside analogs which were previously...

Susceptibility of intra- and extracellular Mycobacterium avium-intracellulare to cephem antibiotics.

Nozawa, R T, Kato, H, Yokota, T, Sugi, H

Intra- and extracellular susceptibility of 35 clinically isolated Mycobacterium avium-intracellulare strains to cefotaxime (CTX), ceftizoxime (CZX), and cefoperazone was studied. MICs for 50% of the...

Intra- and extracellular susceptibility of Mycobacterium avium-intracellulare complex to aminoglycoside antibiotics.

Nozawa, R T, Kato, H, Yokota, T

We developed a rapid, quantitative culture method to estimate the replication of Mycobacterium avium-intracellulare complex (MAIC) in human peripheral blood mononuclear cells. Mononuclear cells were...

Role of an altered penicillin-binding protein in methicillin- and cephem-resistant Staphylococcus aureus.

Utsui, Y, Yokota, T

About 80% of methicillin- and cefazolin-resistant strains of Staphylococcus aureus isolated clinically in Japan in 1982 retained their resistance even after elimination of penicillinase-encoding...

Characterization of a varicella-zoster virus variant with altered thymidine kinase activity.

Shigeta, S, Mori, S, Yokota, T, Konno, K, De Clercq, E

A varicella-zoster virus (VZV) strain resistant to 5-iodo-2'-deoxyuridine (IdUrd) and 5-bromo-2'-deoxyuridine (BrdUrd) but sensitive to (E)-5-(2-bromovinyl)-2'-deoxyuridine (BVdUrd) and...

Antibacterial activity of cefmetazole alone and in combination with fosfomycin against methicillin- and cephem-resistant Staphylococcus aureus.

Utsui, Y, Ohya, S, Magaribuchi, T, Tajima, M, Yokota, T

In vitro and in vivo antibacterial activities of cefmetazole alone and in combination with fosfomycin against methicillin- and cephem-resistant (MR) strains of Staphylococcus aureus were...

Rapid drug susceptibility testing of mycobacteria in tissue culture medium.

Nozawa, R T, Yokota, T

Mycobacteria grew rapidly in FST medium (tissue culture medium F12 supplemented with 5% serum and 0.05% Tween 80). Growth of Mycobacterium tuberculosis and other niacin-negative mycobacteria in...

Interaction between biofilms formed by Pseudomonas aeruginosa and clarithromycin.

Yasuda, H, Ajiki, Y, Koga, T, Kawada, H, Yokota, T

Interactions between bacterial biofilms formed by Pseudomonas aeruginosa and clarithromycin, a macrolide having no anti-P. aeruginosa activity, were investigated. P. aeruginosa incubated for 10 days...

Survey of methicillin-resistant clinical strains of coagulase-negative staphylococci for mecA gene distribution.

Suzuki, E, Hiramatsu, K, Yokota, T

A total number of 125 methicillin-resistant (MIC, greater than or equal to 16) coagulase-negative Staphylococcus strains isolated in Japan were surveyed for the distribution of the mecA gene, the...

Identification of the sequence on NS4A required for enhanced cleavage of the NS5A/5B site by hepatitis C virus NS3 protease.

Shimizu, Y, Yamaji, K, Masuho, Y, Yokota, T, Inoue, H, Sudo, K, ...

In addition to NS3 protease, the NS4A protein is required for efficient cleavage of the nonstructural protein region of the hepatitis C virus polyprotein. To investigate the function and the sequence...

Photostability and biological activity of fluoroquinolones substituted at the 8 position after UV irradiation.

Matsumoto, M, Kojima, K, Nagano, H, Matsubara, S, Yokota, T

Q-35 [1-cyclopropyl-6-fluoro-1,4-dihydro-8-methoxy-7-(3-methylaminopiperid ine-1-yl)-4-oxoquinoline-3-carboxylic acid], a fluoroquinolone, has absorbance peaks at 333 and 286 nm. No spectral change...

Evolutionary origin of pathogenic determinants in enterotoxigenic Escherichia coli and Vibrio cholerae O1.

Yamamoto, T, Gojobori, T, Yokota, T

Three families of the evolutionarily related pathogenic determinants in enterotoxigenic Escherichia coli and Vibrio cholerae O1, a family of cholera enterotoxin (CT) and heat-labile enterotoxin (LT)...

Purification and properties of haloalkane dehalogenase from Corynebacterium sp. strain m15-3.

Yokota, T, Omori, T, Kodama, T

A haloalkane dehalogenase was purified to electrophoretic homogeneity from cell extracts of a 1-chlorobutane-utilizing strain, m15-3, which was identified as a Corynebacterium sp. The enzyme...

Molecular organization of heat-labile enterotoxin genes originating in Escherichia coli of human origin and construction of heat-labile toxoid-producing strains.

Yamamoto, T, Yokota, T, Kaji, A

Recombinant deoxyribonucleic acid technology was employed to construct heat-labile enterotoxin (LT) toxoids. A recombinant plasmid carrying both an LT promoter region and LT subunit A (LTA) gene,...

Release of heat-labile enterotoxin subunits by Escherichia coli.

The region controlling the thermosensitive effect of plasmid Rts1 on host growth is separate from the Rts1 replication region.

Most of the heat-labile enterotoxin (LT) synthesized by Escherichia coli is cell associated; however, a small portion of LT (approximately 10%) is released by bacterial cells into the culture...

Yamamoto, T, Finver, S, Yokota, T, Bricker, J, Kaji, A

Rts1 is a high-molecular-weight (126 x 10(6)) plasmid encoding resistance to kanamycin. It expresses unusual temperature-sensitive phenotypes, which affect plasmid maintenance and replication, as...

Escherichia coli heat-labile enterotoxin genes are flanked by repeated deoxyribonucleic acid sequences.

Sequence analysis of the heat-labile enterotoxin subunit B gene originating in human enterotoxigenic Escherichia coli.

The enterotoxin regions of the heat-labile and heat-stable enterotoxin (LT+ ST+) plasmid, pJY11, originating in a clinically isolated Escherichia coli strain, have been isolated as various-sized...

Yamamoto, T, Tamura, T, Ryoji, M, Kaji, A, Yokota, T, Takano, T

In this study, we determined the amino-terminal coding sequence, covering the signal peptide and the amino-terminus of the mature peptide, of the heat-labile enterotoxin subunit B (LT-B) gene...

Plasmids of enterotoxigenic Escherichia coli H10407: evidence for two heat-stable enterotoxin genes and a conjugal transfer system.

Isolation of TOL and RP4 recombinants by integrative suppression.

Three species of plasmids, associated with virulence and conjugal transfer, were identified in a clinically isolated enterotoxigenic Escherichia coli strain, H10407 (serotype O78:H11). pCS1, a...

Nakazawa, T, Hayashi, E, Yokota, T, Ebina, Y, Nakazawa, A

We obtained genetic and molecular evidence of non-thermosensitive recombinants of RP4 (Kmr Tcr Cbr/Apr) and the thermosensitive TOL plasmid. As first isolated in Pseudomonas aeruginosa PAO, the...

Isolation of a mutant TOL plasmid with increased activity and transmissibility from Pseudomonas putida (arvilla) mt-2.

Nakazawa, T, Yokota, T

Strains with greater ability to dissimilate m-toluate were obtained from the wild-type Pseudomonas putida (arvilla) mt-2 that harbors the TOL plasmid. Increased growth of a mutant strain on aromatic...

Host-dependent, thermosensitive replication of an R plasmid, pJY5, isolated from Enterobacter cloacae.

Identification of a methicillin-resistant strain of Staphylococcus caprae from a human clinical specimen.

The thermosensitive replication of an R plasmid, pJY5, isolated from Enterobacter cloacae, was studied. pJY5 consisted of 61 million daltons of covalently closed circular (CCC) deoxyribonucleic acid...

Kanda, K, Suzuki, E, Hiramatsu, K, Oguri, T, Miura, H, Ezaki, T, ...

The analysis of gel banding patterns of penicillin-binding proteins was used to identify two clinical isolates of a coagulase-negative Staphylococcus species as Staphylococcus caprae, a species...

Inhibitory effects of selected antiviral compounds on human hepatitis B virus DNA synthesis.

Yokota, T, Mochizuki, S, Konno, K, Mori, S, Shigeta, S, De Clercq, E

By using an assay system based on a human hepatoblastoma cell line (HB611) that continuously synthesizes hepatitis B virus DNA, the following compounds were found to inhibit hepatitis B virus DNA...

Characteristics of adherence of enteroaggregative Escherichia coli to human and animal mucosa.

Yamamoto, T, Endo, S, Yokota, T, Echeverria, P

An Escherichia coli strain (serotype O127a:H2) that had been isolated from a child with diarrhea in Thailand and that was negative for the virulence factors of the four categories of diarrheagenic E....

Electron microscopic study of Vibrio cholerae O1 adherence to the mucus coat and villus surface in the human small intestine.

Vibrio cholerae O1 adherence to villi and lymphoid follicle epithelium: in vitro model using formalin-treated human small intestine and correlation between adherence and cell-associated hemagglutinin levels.

Vibrio cholerae O1, irrespective of the biotype or serotype, adhered to and was entrapped in the mucus coat covering the mucosal surface of isolated human ileal segments. The evidence for such mucus...

Yamamoto, T, Kamano, T, Uchimura, M, Iwanaga, M, Yokota, T

Formalin-fixed human small intestinal mucosa possessing villi and lymphoid follicle epithelium of Peyer's patches at the mucosal surface was used to test the adherence ability of clinically isolated...

Protective effect of human granulocyte colony-stimulating factor on microbial infection in neutropenic mice.

Matsumoto, M, Matsubara, S, Matsuno, T, Tamura, M, Hattori, K, Nomura, H, ...

A purified human granulocyte colony-stimulating factor (hG-CSF) was studied for its protective effect on the induction of neutropenia and enhanced susceptibility to microbial infections in mice...

Enteroadhesion fimbriae and enterotoxin of Escherichia coli: genetic transfer to a streptomycin-resistant mutant of the galE oral-route live-vaccine Salmonella typhi Ty21a.

Vibrio cholerae non-O1: production of cell-associated hemagglutinins and in vitro adherence to mucus coat and epithelial surfaces of the villi and lymphoid follicles of human small intestines treated with formalin.

An enterotoxigenic Escherichia coli plasmid encoding colonization factor antigen (CFA) I fimbriae and heat-stable toxin was transferred into a streptomycin-resistant mutant of the Salmonella typhi...

Monoclonal antibodies to Vibrio cholerae O1 serotype inaba.

Clinically isolated Vibrio cholerae non-O1 strains produced more cell-associated hemagglutinins (HAs) on colonization factor antigen agar after ca. 3 h than after ca. 20 h of incubation at 37 degrees...

Different types of monoclonal antibodies to Ogawa-specific and group-specific antigens of Vibrio cholerae O1.

Eighteen monoclonal antibodies were generated from mice immunized with Vibrio cholerae O1 serotype Inaba. Reactivities of these antibodies were investigated by slide agglutination, microdilution...

Automatic and manual latex agglutination tests for measurement of cholera toxin and heat-labile enterotoxin of Escherichia coli.

Nine monoclonal antibodies to Ogawa-specific antigenic determinants of Vibrio cholerae O1 and seven monoclonal antibodies to the Ogawa-Inaba common antigenic determinants were obtained. Specificities...

Ito, T, Kuwahara, S, Yokota, T

Automated and manual latex agglutination methods were employed to measure cholera toxin (CT), heat-labile enterotoxin (LT) of Escherichia coli, and their subunits A and B. Dow polystyrene latex...

Assay method for Vibrio cholerae and Escherichia coli enterotoxins by automated counting of floating chinese hamster ovary cells in culture medium.

Nozawa, R T, Yokota, T, Kuwahara, S

As Chinese hamster ovary (CHO) cells on plastic proliferate, many cells float off into the medium instead of piling up after they form a monolayer. Fewer cells were floating in the medium when CHO...

Differential regulation of early response genes and cell proliferation through the human granulocyte macrophage colony-stimulating factor receptor: selective activation of the c-fos promoter by genistein.

Watanabe, S, Muto, A, Yokota, T, Miyajima, A, Arai, K

Granulocyte macrophage colony-stimulating factor (GM-CSF) binds to the high-affinity GM-CSF receptor (GMR) consisting of alpha and beta subunits and induces rapid tyrosine phosphorylation, activation...

Calcineurin-dependent nuclear translocation of a murine transcription factor NFATx: molecular cloning and functional characterization.

Liu, J, Koyano-Nakagawa, N, Amasaki, Y, Saito-Ohara, F, Ikeuchi, T, Imai, S, ...

Members of the nuclear factor of activated T cells (NFAT) are involved in the induction of a number of cytokine genes. We report here cDNA cloning and chromosomal localization of a murine homologue...

IgE production by normal human lymphocytes is induced by interleukin 4 and suppressed by interferons gamma and alpha and prostaglandin E2.

Pène, J, Rousset, F, Brière, F, Chrétien, I, Bonnefoy, J Y, Spits, H, ...

The effect of human recombinant interleukin 4 (IL-4) on antibody production by normal peripheral blood mononuclear cells enriched for B cells was investigated. IL-4 preferentially induced IgE...

Interaction between clarithromycin and biofilms formed by Staphylococcus epidermidis.

Yasuda, H, Ajiki, Y, Koga, T, Yokota, T

Interactions between clarithromycin and biofilms formed by Staphylococcus epidermidis were investigated by using a clarithromycin-resistant strain. Treatment of the colonization with a relatively low...

Cloning of deoxyribonucleic acid regions encoding a heat-labile and heat-stable enterotoxin originating from an enterotoxigenic Escherichia coli strain of human origin.

Isolation and characterization of lymphokine cDNA clones encoding mouse and human IgA-enhancing factor and eosinophil colony-stimulating factor activities: relationship to interleukin 5.

A heat-labile and heat-stable enterotoxin (LT+ ST+) plasmic (62.7 kilobases in size) was isolated from an enterotoxigenic Escherichia coli human strain, H10407, and used for analysis of the LT+ and...

Yokota, T, Coffman, R L, Hagiwara, H, Rennick, D M, Takebe, Y, Yokota, K, ...

Conditioned medium from the Con A-treated mouse helper T-cell clone Ly1+2-/9 contains activities that enhance the production of IgA by mouse B cells and induce human cord blood cells to form...

A human GM-CSF receptor expressed in transgenic mice stimulates proliferation and differentiation of hemopoietic progenitors to all lineages in response to human GM-CSF.

Nishijima, I, Nakahata, T, Hirabayashi, Y, Inoue, T, Kurata, H, Miyajima, A, ...

Granulocyte-macrophage colony-stimulating factor (GM-CSF) mainly stimulates proliferation and maturation of myeloid progenitor cells. Although the signal transduction pathways triggered by GM-CSF...

Isolation and characterization of bovine and mouse terminal deoxynucleotidyltransferase cDNAs expressible in mammalian cells.

Koiwai, O, Yokota, T, Kageyama, T, Hirose, T, Yoshida, S, Arai, K

We have isolated nearly full-length cDNA clones of terminal deoxynucleotidyltransferase (TdT) from calf thymus and mouse lymphoma cDNA libraries. The libraries were constructed using the pcD vector...

Adherence targets of Vibrio parahaemolyticus in human small intestines.

In vitro adherence of type 1-fimbriated uropathogenic Escherichia coli to human ureteral mucosa.

Formalin-fixed human small intestinal mucosa with mucus coating, villi, and lymphoid follicle epithelium at the mucosal surface was used to test the adherence sites of clinically isolated (Kanagawa...

Fujita, K, Yamamoto, T, Yokota, T, Kitagawa, R

Type 1-fimbriated Escherichia coli isolated from patients with urinary tract infections adhered in vitro to the epithelial cell surface of an excised human ureter. The bacteria also adhered to a...

Isolation and characterization of a mouse interleukin cDNA clone that expresses B-cell stimulatory factor 1 activities and T-cell- and mast-cell-stimulating activities.

Lee, F, Yokota, T, Otsuka, T, Meyerson, P, Villaret, D, Coffman, R, ...

A cDNA sequence coding for a unique mouse interleukin that expresses B-cell-, T-cell, and mast-cell-stimulating activities has been isolated from a mouse helper T-cell cDNA library. The library,...

Activation of T cell-derived lymphokine genes in T cells and fibroblasts: effects of human T cell leukemia virus type I p40x protein and bovine papilloma virus encoded E2 protein.

Miyatake, S, Seiki, M, Malefijt, R D, Heike, T, Fujisawa, J, Takebe, Y, ...

The effects of p40x, a product of an human T cell leukemia virus type I, on the activation of lymphokine genes were examined. The mouse GM-CSF and IL-3 genes were activated by cotransfection with a...

Structural analysis of the mouse chromosomal gene encoding interleukin 4 which expresses B cell, T cell and mast cell stimulating activities.

Otsuka, T, Villaret, D, Yokota, T, Takebe, Y, Lee, F, Arai, N, ...

Based on homology with the mouse interleukin 4 (IL-4) cDNA that expresses B cell, T cell, and mast cell stimulating activities (Lee, F. et al., (1986) Proc. Natl. Acad. Sci. USA 83, 2061), we have...

Isolation and characterization of a mouse cDNA clone that expresses mast-cell growth-factor activity in monkey cells.

Yokota, T, Lee, F, Rennick, D, Hall, C, Arai, N, Mosmann, T, ...

A cDNA sequence coding for mouse mast-cell growth-factor (MCGF) has been cloned from a cDNA library prepared from mRNA derived from a concanavalin A-activated mouse T-cell clone. Cloning was achieved...

Molecular cloning of a novel human cDNA encoding a zinc finger protein that binds to the interleukin-3 promoter.

Koyano-Nakagawa, N, Nishida, J, Baldwin, D, Arai, K, Yokota, T

The CT/GC-rich region (-76 to -47) is one transcriptional regulatory region of the interleukin-3 (IL-3) gene which confers basic transcriptional activity and responds to trans-activation by human...

Isolation and characterization of a human interleukin cDNA clone, homologous to mouse B-cell stimulatory factor 1, that expresses B-cell- and T-cell-stimulating activities.

Yokota, T, Otsuka, T, Mosmann, T, Banchereau, J, DeFrance, T, Blanchard, D, ...

A cDNA sequence coding for a human interleukin has been isolated from a concanavalin A-activated human T-cell cDNA library based on homology with a mouse interleukin cDNA that expresses B-cell...

Isolation of cDNA for a human granulocyte-macrophage colony-stimulating factor by functional expression in mammalian cells.

Lee, F, Yokota, T, Otsuka, T, Gemmell, L, Larson, N, Luh, J, ...

A cDNA sequence coding for a human granulocyte-macrophage colony-stimulating factor has been isolated from cDNA libraries prepared from mRNA derived from concanavalin A-activated human T-cell clones....

ATL-derived factor (ADF), an IL-2 receptor/Tac inducer homologous to thioredoxin; possible involvement of dithiol-reduction in the IL-2 receptor induction.

Tagaya, Y, Maeda, Y, Mitsui, A, Kondo, N, Matsui, H, Hamuro, J, ...

Use of a cDNA expression vector for isolation of mouse interleukin 2 cDNA clones: expression of T-cell growth-factor activity after transfection of monkey cells.

Yokota, T, Arai, N, Lee, F, Rennick, D, Mosmann, T, Arai, K

A cDNA sequence coding for mouse interleukin 2 (IL-2) has been cloned from a cDNA library prepared from mRNA derived from a concanavalin A-activated mouse T-cell clone. The library was constructed by...

Structure of the chromosomal gene for murine interleukin 3.

Miyatake, S, Yokota, T, Lee, F, Arai, K

We have isolated the mouse interleukin 3 (IL-3) gene from a mouse sperm DNA library based on homology with the mouse mast-cell growth-factor (MCGF) cDNA sequence. The nucleotide sequence determined...

Suppression of apoptotic death in hematopoietic cells by signalling through the IL-3/GM-CSF receptors.

Kinoshita, T, Yokota, T, Arai, K, Miyajima, A

Interleukin 3 (IL-3) and granulocyte-macrophage colony stimulating factor (GM-CSF) exert their biological functions through acting on a specific receptor which consists of a ligand-specific alpha...

ATL-derived factor (ADF), an IL-2 receptor/Tac inducer homologous to thioredoxin; possible involvement of dithiol-reduction in the IL-2 receptor induction.

Tagaya, Y, Maeda, Y, Mitsui, A, Kondo, N, Matsui, H, Hamuro, J, ...

HTLV-I transformed T cells not only express a large number of interleukin-2 receptors [IL-2R/p55(Tac)], but also produce a factor named ATL-derived factor (ADF) that augments the expression of...

Increased adhesion of Chinese hamster ovary cells to substratum by cholera enterotoxin.

Nozawa, R, Kon, F, Yokota, T, Ohashi, M, Kuwahara, S

A new, simple assay method using Chinese hamster ovary cells was devised for cholera enterotoxin. The effect of the cholera toxin was measured by an increase in cell adhesion to the substratum. The...

Systemic reactive amyloidosis associated with Castleman's disease: serial changes of the concentrations of acute phase serum amyloid A and interleukin 6 in serum.

Ikeda, S, Chisuwa, H, Kawasaki, S, Ozawa, J, Hoshii, Y, Yokota, T, ...

A case is reported of a 21 year old woman who suffered from Castleman's disease and systemic reactive amyloidosis. The serum concentrations of serum amyloid A (SAA) and interleukin 6 (IL-6) were...

Structure of the chromosomal gene for granulocyte-macrophage colony stimulating factor: comparison of the mouse and human genes.

Miyatake, S, Otsuka, T, Yokota, T, Lee, F, Arai, K

A cDNA clone that expresses granulocyte-macrophage colony stimulating factor (GM-CSF) activity in COS-7 cells has been isolated from a pcD library prepared from mRNA derived from concanavalin...

STAT3 activation is sufficient to maintain an undifferentiated state of mouse embryonic stem cells.

Matsuda, T, Nakamura, T, Nakao, K, Arai, T, Katsuki, M, Heike, T, ...

Embryonic stem (ES) cells can be maintained in an undifferentiated state in the presence of leukemia inhibitory factor (LIF). LIF acts through a receptor complex composed of a low affinity LIF...

Sequence of heat-labile enterotoxin of Escherichia coli pathogenic for humans.

Effects of 8-substituted analogs of cyclic adenosine 3',5'-monophosphate on in vivo and in vitro syntheses of beta-galactosidase in Escherichia coli.

We determined the complete nucleotide sequence of the toxB gene (375 base pairs in length), which encodes the B subunit of heat-labile enterotoxin produced from Escherichia coli pathogenic for humans...

Ito, T, Yokota, T, Sasaki, Y, Suzuki, N, Sowa, T

Several 8-substituted alkylthio and alkylamino cyclic adenosine 3',5'-monophosphate (cAMP) derivatives were tested for their ability to stimulate beta-galactosidase synthesis in Estherichia coli in...

Reconstituted human granulocyte-macrophage colony-stimulating factor receptor transduces growth-promoting signals in mouse NIH 3T3 cells: comparison with signalling in BA/F3 pro-B cells.

Watanabe, S, Mui, A L, Muto, A, Chen, J X, Hayashida, K, Yokota, T, ...

Granulocyte-macrophage colony-stimulating factor (GM-CSF) plays a critical role in growth and differentiation of myeloid cells. We previously reconstituted high-affinity human GM-CSF receptor...

Depolarization of afferent terminals evoked by muscle stretch.

Muscle stretch and the presynaptic inhibition of the group Ia pathway to motoneurones.

Somatic sensory cortical projection areas excited by tactile stimulation of the cat: a triple representation

Darian-Smith, I., Isbister, J., Mok, H., Yokota, T.

1. The specific somatic sensory projection from the face to the cerebral cortex of the cat was investigated in two series of experiments. α-chloralose and Nembutal were the anaesthetics used.

The effect of acetylcholine upon mammalian motor nerve terminals.

Hubbard, J I, Schmidt, R F, Yokota, T

Conversion of G-protein specificity of insulin-like growth factor II/mannose 6-phosphate receptor by exchanging of a short region with beta-adrenergic receptor.

Takahashi, K, Murayama, Y, Okamoto, T, Yokota, T, Ikezu, T, Takahashi, S, ...

The 14-residue peptide (peptide 14) corresponding to Arg2410-Lys2423 of the insulin-like growth factor II receptor (IGF-IIR) can activate the adenylate cyclase-inhibitor guanine nucleotide-binding...

Reconstitution of functional receptors for human granulocyte/macrophage colony-stimulating factor (GM-CSF): evidence that the protein encoded by the AIC2B cDNA is a subunit of the murine GM-CSF receptor.

Kitamura, T, Hayashida, K, Sakamaki, K, Yokota, T, Arai, K, Miyajima, A

The high-affinity receptor for human granulocyte/macrophage colony-stimulating factor (hGM-CSF) is composed of two subunits, alpha and beta. The alpha subunit binds GM-CSF with low affinity, whereas...

Chimeric opioid peptides: tools for identifying opioid receptor types.

Xie, G X, Miyajima, A, Yokota, T, Arai, K, Goldstein, A

We synthesized several chimeric peptides in which the N-terminal nine residues of dynorphin-32, a peptide selective for the kappa opioid receptor, were replaced by opioid peptides selective for other...

Molecular cloning of a second subunit of the receptor for human granulocyte-macrophage colony-stimulating factor (GM-CSF): reconstitution of a high-affinity GM-CSF receptor.

Hayashida, K, Kitamura, T, Gorman, D M, Arai, K, Yokota, T, Miyajima, A

Using the mouse interleukin 3 (IL-3) receptor cDNA as a probe, we obtained a homologous cDNA (KH97) from a cDNA library of a human hemopoietic cell line, TF-1. The protein encoded by the KH97 cDNA...

The Arabidopsis DIMINUTO/DWARF1 gene encodes a protein involved in steroid synthesis.

Klahre, U, Noguchi, T, Fujioka, S, Takatsuto, S, Yokota, T, Nomura, T, ...

We have identified the function of the Arabidopsis DIMINUTO/DWARF1 (DIM/DWF1) gene by analyzing the dim mutant, a severe dwarf with greatly reduced fertility. Both the mutant phenotype and gene...

The Arabidopsis deetiolated2 mutant is blocked early in brassinosteroid biosynthesis.

Fujioka, S, Li, J, Choi, Y H, Seto, H, Takatsuto, S, Noguchi, T, ...

The Arabidopsis DEETIOLATED2 (DET2) gene has been cloned and shown to encode a protein that shares significant sequence identity with mammalian steroid 5 alpha-reductases. Loss of DET2 function...

Blockage of Brassinosteroid Biosynthesis and Sensitivity Causes Dwarfism in Garden Pea.

Nomura, T., Nakayama, M., Reid, J. B., Takeuchi, Y., Yokota, T.

Endogenous brassinosteroids (BRs) in the dwarf mutants lka and lkb of garden pea (Pisum sativum L.) and comparable wild-type plants were quantified by gas chromatography-selected ion monitoring using...

Mechanism of selective inhibition of human immunodeficiency virus by ingenol triacetate.

Fujiwara, M, Ijichi, K, Tokuhisa, K, Katsuura, K, Shigeta, S, Konno, K, ...

Ingenol 3,5,20-triacetate (ITA), one of the ingenol derivatives, is a selective inhibitor of human immunodeficiency virus (HIV) replication in vitro. ITA inhibited the replication of HIV strains in...

Role of penicillinase plasmids in the stability of the mecA gene in methicillin-resistant Staphylococcus aureus.

Hiramatsu, K, Suzuki, E, Takayama, H, Katayama, Y, Yokota, T

The stability of methicillin resistance (Mcr) in three independent clinical isolates, MR108, MR6, and MR61, of methicillin-resistant Staphylococcus aureus (MRSA) was studied. The Mcr phenotype was...

In vitro susceptibilities of Pseudomonas pseudomallei to 27 antimicrobial agents.

Yamamoto, T, Naigowit, P, Dejsirilert, S, Chiewsilp, D, Kondo, E, Yokota, T, ...

Clinical isolates of Pseudomonas pseudomallei isolated in Thailand from 1981 to 1989 were tested for their in vitro susceptibilities to 27 antimicrobial agents, including older and newer quinolones,...

Comparative activities of several nucleoside analogs against influenza A, B, and C viruses in vitro.

Shigeta, S, Konno, K, Yokota, T, Nakamura, K, De Clercq, E

A set of 20 nucleoside analogs were examined for their inhibitory effects on the cytopathogenicity and growth of influenza virus type A, B, and C strains in Madin-Darby canine kidney (MDCK) cells....

Antiseptic and antibiotic resistance plasmid in Staphylococcus aureus that possesses ability to confer chlorhexidine and acrinol resistance.

Susceptibility of methicillin-resistant Staphylococcus aureus to the selenium-containing compound 2-phenyl-1,2-benzoisoselenazol-3(2H)-one (PZ51).

Plasmid pSAJ1 from a methicillin- and gentamicin-resistant strain of Staphylococcus aureus had am molecular size of 50 kilobases and conferred resistance not only to kanamycin, gentamicin,...

Nozawa, R, Yokota, T, Fujimoto, T

The growth of Staphylococcus aureus 209P was inhibited by 0.20 micrograms of 2-phenyl-1,2-benzoisoselenazol-3(2H)-one (PZ51) per ml, while strains of the family Enterobacteriaceae were more resistant...

Comparative activities of several nucleoside analogs against duck hepatitis B virus in vitro.

Yokota, T, Konno, K, Chonan, E, Mochizuki, S, Kojima, K, Shigeta, S, ...

Duck hepatitis B virus (DHBV) replication in primary duck hepatocytes was monitored by examining the synthesis of both DHBV DNA and DHBV core antigen. Several nucleoside analogs which were previously...

Susceptibility of intra- and extracellular Mycobacterium avium-intracellulare to cephem antibiotics.

Nozawa, R T, Kato, H, Yokota, T, Sugi, H

Intra- and extracellular susceptibility of 35 clinically isolated Mycobacterium avium-intracellulare strains to cefotaxime (CTX), ceftizoxime (CZX), and cefoperazone was studied. MICs for 50% of the...

Intra- and extracellular susceptibility of Mycobacterium avium-intracellulare complex to aminoglycoside antibiotics.

Nozawa, R T, Kato, H, Yokota, T

We developed a rapid, quantitative culture method to estimate the replication of Mycobacterium avium-intracellulare complex (MAIC) in human peripheral blood mononuclear cells. Mononuclear cells were...

Role of an altered penicillin-binding protein in methicillin- and cephem-resistant Staphylococcus aureus.

Utsui, Y, Yokota, T

About 80% of methicillin- and cefazolin-resistant strains of Staphylococcus aureus isolated clinically in Japan in 1982 retained their resistance even after elimination of penicillinase-encoding...

Characterization of a varicella-zoster virus variant with altered thymidine kinase activity.

Shigeta, S, Mori, S, Yokota, T, Konno, K, De Clercq, E

A varicella-zoster virus (VZV) strain resistant to 5-iodo-2'-deoxyuridine (IdUrd) and 5-bromo-2'-deoxyuridine (BrdUrd) but sensitive to (E)-5-(2-bromovinyl)-2'-deoxyuridine (BVdUrd) and...

Antibacterial activity of cefmetazole alone and in combination with fosfomycin against methicillin- and cephem-resistant Staphylococcus aureus.

Utsui, Y, Ohya, S, Magaribuchi, T, Tajima, M, Yokota, T

In vitro and in vivo antibacterial activities of cefmetazole alone and in combination with fosfomycin against methicillin- and cephem-resistant (MR) strains of Staphylococcus aureus were...

Rapid drug susceptibility testing of mycobacteria in tissue culture medium.

Nozawa, R T, Yokota, T

Mycobacteria grew rapidly in FST medium (tissue culture medium F12 supplemented with 5% serum and 0.05% Tween 80). Growth of Mycobacterium tuberculosis and other niacin-negative mycobacteria in...

Interaction between biofilms formed by Pseudomonas aeruginosa and clarithromycin.

Yasuda, H, Ajiki, Y, Koga, T, Kawada, H, Yokota, T

Interactions between bacterial biofilms formed by Pseudomonas aeruginosa and clarithromycin, a macrolide having no anti-P. aeruginosa activity, were investigated. P. aeruginosa incubated for 10 days...

Survey of methicillin-resistant clinical strains of coagulase-negative staphylococci for mecA gene distribution.

Suzuki, E, Hiramatsu, K, Yokota, T

A total number of 125 methicillin-resistant (MIC, greater than or equal to 16) coagulase-negative Staphylococcus strains isolated in Japan were surveyed for the distribution of the mecA gene, the...

Identification of the sequence on NS4A required for enhanced cleavage of the NS5A/5B site by hepatitis C virus NS3 protease.

Shimizu, Y, Yamaji, K, Masuho, Y, Yokota, T, Inoue, H, Sudo, K, ...

In addition to NS3 protease, the NS4A protein is required for efficient cleavage of the nonstructural protein region of the hepatitis C virus polyprotein. To investigate the function and the sequence...

Photostability and biological activity of fluoroquinolones substituted at the 8 position after UV irradiation.

Matsumoto, M, Kojima, K, Nagano, H, Matsubara, S, Yokota, T

Q-35 [1-cyclopropyl-6-fluoro-1,4-dihydro-8-methoxy-7-(3-methylaminopiperid ine-1-yl)-4-oxoquinoline-3-carboxylic acid], a fluoroquinolone, has absorbance peaks at 333 and 286 nm. No spectral change...

Evolutionary origin of pathogenic determinants in enterotoxigenic Escherichia coli and Vibrio cholerae O1.

Yamamoto, T, Gojobori, T, Yokota, T

Three families of the evolutionarily related pathogenic determinants in enterotoxigenic Escherichia coli and Vibrio cholerae O1, a family of cholera enterotoxin (CT) and heat-labile enterotoxin (LT)...

Purification and properties of haloalkane dehalogenase from Corynebacterium sp. strain m15-3.

Yokota, T, Omori, T, Kodama, T

A haloalkane dehalogenase was purified to electrophoretic homogeneity from cell extracts of a 1-chlorobutane-utilizing strain, m15-3, which was identified as a Corynebacterium sp. The enzyme...

Molecular organization of heat-labile enterotoxin genes originating in Escherichia coli of human origin and construction of heat-labile toxoid-producing strains.

Yamamoto, T, Yokota, T, Kaji, A

Recombinant deoxyribonucleic acid technology was employed to construct heat-labile enterotoxin (LT) toxoids. A recombinant plasmid carrying both an LT promoter region and LT subunit A (LTA) gene,...

Release of heat-labile enterotoxin subunits by Escherichia coli.

The region controlling the thermosensitive effect of plasmid Rts1 on host growth is separate from the Rts1 replication region.

Most of the heat-labile enterotoxin (LT) synthesized by Escherichia coli is cell associated; however, a small portion of LT (approximately 10%) is released by bacterial cells into the culture...

Yamamoto, T, Finver, S, Yokota, T, Bricker, J, Kaji, A

Rts1 is a high-molecular-weight (126 x 10(6)) plasmid encoding resistance to kanamycin. It expresses unusual temperature-sensitive phenotypes, which affect plasmid maintenance and replication, as...

Escherichia coli heat-labile enterotoxin genes are flanked by repeated deoxyribonucleic acid sequences.

Sequence of heat-labile enterotoxin of Escherichia coli pathogenic for humans.

The enterotoxin regions of the heat-labile and heat-stable enterotoxin (LT+ ST+) plasmid, pJY11, originating in a clinically isolated Escherichia coli strain, have been isolated as various-sized...

Effects of 8-substituted analogs of cyclic adenosine 3',5'-monophosphate on in vivo and in vitro syntheses of beta-galactosidase in Escherichia coli.

We determined the complete nucleotide sequence of the toxB gene (375 base pairs in length), which encodes the B subunit of heat-labile enterotoxin produced from Escherichia coli pathogenic for humans...

Ito, T, Yokota, T, Sasaki, Y, Suzuki, N, Sowa, T

Several 8-substituted alkylthio and alkylamino cyclic adenosine 3',5'-monophosphate (cAMP) derivatives were tested for their ability to stimulate beta-galactosidase synthesis in Estherichia coli in...

Sequence analysis of the heat-labile enterotoxin subunit B gene originating in human enterotoxigenic Escherichia coli.

Yamamoto, T, Tamura, T, Ryoji, M, Kaji, A, Yokota, T, Takano, T

In this study, we determined the amino-terminal coding sequence, covering the signal peptide and the amino-terminus of the mature peptide, of the heat-labile enterotoxin subunit B (LT-B) gene...

Plasmids of enterotoxigenic Escherichia coli H10407: evidence for two heat-stable enterotoxin genes and a conjugal transfer system.

Isolation of TOL and RP4 recombinants by integrative suppression.

Three species of plasmids, associated with virulence and conjugal transfer, were identified in a clinically isolated enterotoxigenic Escherichia coli strain, H10407 (serotype O78:H11). pCS1, a...

Nakazawa, T, Hayashi, E, Yokota, T, Ebina, Y, Nakazawa, A

We obtained genetic and molecular evidence of non-thermosensitive recombinants of RP4 (Kmr Tcr Cbr/Apr) and the thermosensitive TOL plasmid. As first isolated in Pseudomonas aeruginosa PAO, the...

Isolation of a mutant TOL plasmid with increased activity and transmissibility from Pseudomonas putida (arvilla) mt-2.

Nakazawa, T, Yokota, T

Strains with greater ability to dissimilate m-toluate were obtained from the wild-type Pseudomonas putida (arvilla) mt-2 that harbors the TOL plasmid. Increased growth of a mutant strain on aromatic...

Host-dependent, thermosensitive replication of an R plasmid, pJY5, isolated from Enterobacter cloacae.

Identification of a methicillin-resistant strain of Staphylococcus caprae from a human clinical specimen.

The thermosensitive replication of an R plasmid, pJY5, isolated from Enterobacter cloacae, was studied. pJY5 consisted of 61 million daltons of covalently closed circular (CCC) deoxyribonucleic acid...

Kanda, K, Suzuki, E, Hiramatsu, K, Oguri, T, Miura, H, Ezaki, T, ...

The analysis of gel banding patterns of penicillin-binding proteins was used to identify two clinical isolates of a coagulase-negative Staphylococcus species as Staphylococcus caprae, a species...

Inhibitory effects of selected antiviral compounds on human hepatitis B virus DNA synthesis.

Yokota, T, Mochizuki, S, Konno, K, Mori, S, Shigeta, S, De Clercq, E

By using an assay system based on a human hepatoblastoma cell line (HB611) that continuously synthesizes hepatitis B virus DNA, the following compounds were found to inhibit hepatitis B virus DNA...

Characteristics of adherence of enteroaggregative Escherichia coli to human and animal mucosa.

Yamamoto, T, Endo, S, Yokota, T, Echeverria, P

An Escherichia coli strain (serotype O127a:H2) that had been isolated from a child with diarrhea in Thailand and that was negative for the virulence factors of the four categories of diarrheagenic E....

Electron microscopic study of Vibrio cholerae O1 adherence to the mucus coat and villus surface in the human small intestine.

Vibrio cholerae O1 adherence to villi and lymphoid follicle epithelium: in vitro model using formalin-treated human small intestine and correlation between adherence and cell-associated hemagglutinin levels.

Vibrio cholerae O1, irrespective of the biotype or serotype, adhered to and was entrapped in the mucus coat covering the mucosal surface of isolated human ileal segments. The evidence for such mucus...

Yamamoto, T, Kamano, T, Uchimura, M, Iwanaga, M, Yokota, T

Formalin-fixed human small intestinal mucosa possessing villi and lymphoid follicle epithelium of Peyer's patches at the mucosal surface was used to test the adherence ability of clinically isolated...

Protective effect of human granulocyte colony-stimulating factor on microbial infection in neutropenic mice.

Matsumoto, M, Matsubara, S, Matsuno, T, Tamura, M, Hattori, K, Nomura, H, ...

A purified human granulocyte colony-stimulating factor (hG-CSF) was studied for its protective effect on the induction of neutropenia and enhanced susceptibility to microbial infections in mice...

Enteroadhesion fimbriae and enterotoxin of Escherichia coli: genetic transfer to a streptomycin-resistant mutant of the galE oral-route live-vaccine Salmonella typhi Ty21a.

Vibrio cholerae non-O1: production of cell-associated hemagglutinins and in vitro adherence to mucus coat and epithelial surfaces of the villi and lymphoid follicles of human small intestines treated with formalin.

An enterotoxigenic Escherichia coli plasmid encoding colonization factor antigen (CFA) I fimbriae and heat-stable toxin was transferred into a streptomycin-resistant mutant of the Salmonella typhi...

Monoclonal antibodies to Vibrio cholerae O1 serotype inaba.

Clinically isolated Vibrio cholerae non-O1 strains produced more cell-associated hemagglutinins (HAs) on colonization factor antigen agar after ca. 3 h than after ca. 20 h of incubation at 37 degrees...

Different types of monoclonal antibodies to Ogawa-specific and group-specific antigens of Vibrio cholerae O1.

Eighteen monoclonal antibodies were generated from mice immunized with Vibrio cholerae O1 serotype Inaba. Reactivities of these antibodies were investigated by slide agglutination, microdilution...

Automatic and manual latex agglutination tests for measurement of cholera toxin and heat-labile enterotoxin of Escherichia coli.

Nine monoclonal antibodies to Ogawa-specific antigenic determinants of Vibrio cholerae O1 and seven monoclonal antibodies to the Ogawa-Inaba common antigenic determinants were obtained. Specificities...

Ito, T, Kuwahara, S, Yokota, T

Automated and manual latex agglutination methods were employed to measure cholera toxin (CT), heat-labile enterotoxin (LT) of Escherichia coli, and their subunits A and B. Dow polystyrene latex...

Assay method for Vibrio cholerae and Escherichia coli enterotoxins by automated counting of floating chinese hamster ovary cells in culture medium.

Nozawa, R T, Yokota, T, Kuwahara, S

As Chinese hamster ovary (CHO) cells on plastic proliferate, many cells float off into the medium instead of piling up after they form a monolayer. Fewer cells were floating in the medium when CHO...

Differential regulation of early response genes and cell proliferation through the human granulocyte macrophage colony-stimulating factor receptor: selective activation of the c-fos promoter by genistein.

Watanabe, S, Muto, A, Yokota, T, Miyajima, A, Arai, K

Granulocyte macrophage colony-stimulating factor (GM-CSF) binds to the high-affinity GM-CSF receptor (GMR) consisting of alpha and beta subunits and induces rapid tyrosine phosphorylation, activation...

Calcineurin-dependent nuclear translocation of a murine transcription factor NFATx: molecular cloning and functional characterization.

Liu, J, Koyano-Nakagawa, N, Amasaki, Y, Saito-Ohara, F, Ikeuchi, T, Imai, S, ...

Members of the nuclear factor of activated T cells (NFAT) are involved in the induction of a number of cytokine genes. We report here cDNA cloning and chromosomal localization of a murine homologue...

IgE production by normal human lymphocytes is induced by interleukin 4 and suppressed by interferons gamma and alpha and prostaglandin E2.

Pène, J, Rousset, F, Brière, F, Chrétien, I, Bonnefoy, J Y, Spits, H, ...

The effect of human recombinant interleukin 4 (IL-4) on antibody production by normal peripheral blood mononuclear cells enriched for B cells was investigated. IL-4 preferentially induced IgE...

Interaction between clarithromycin and biofilms formed by Staphylococcus epidermidis.

Yasuda, H, Ajiki, Y, Koga, T, Yokota, T

Interactions between clarithromycin and biofilms formed by Staphylococcus epidermidis were investigated by using a clarithromycin-resistant strain. Treatment of the colonization with a relatively low...

Cloning of deoxyribonucleic acid regions encoding a heat-labile and heat-stable enterotoxin originating from an enterotoxigenic Escherichia coli strain of human origin.

Isolation and characterization of lymphokine cDNA clones encoding mouse and human IgA-enhancing factor and eosinophil colony-stimulating factor activities: relationship to interleukin 5.

A heat-labile and heat-stable enterotoxin (LT+ ST+) plasmic (62.7 kilobases in size) was isolated from an enterotoxigenic Escherichia coli human strain, H10407, and used for analysis of the LT+ and...

Yokota, T, Coffman, R L, Hagiwara, H, Rennick, D M, Takebe, Y, Yokota, K, ...

Conditioned medium from the Con A-treated mouse helper T-cell clone Ly1+2-/9 contains activities that enhance the production of IgA by mouse B cells and induce human cord blood cells to form...

A human GM-CSF receptor expressed in transgenic mice stimulates proliferation and differentiation of hemopoietic progenitors to all lineages in response to human GM-CSF.

Nishijima, I, Nakahata, T, Hirabayashi, Y, Inoue, T, Kurata, H, Miyajima, A, ...

Granulocyte-macrophage colony-stimulating factor (GM-CSF) mainly stimulates proliferation and maturation of myeloid progenitor cells. Although the signal transduction pathways triggered by GM-CSF...

Isolation and characterization of bovine and mouse terminal deoxynucleotidyltransferase cDNAs expressible in mammalian cells.

Koiwai, O, Yokota, T, Kageyama, T, Hirose, T, Yoshida, S, Arai, K

We have isolated nearly full-length cDNA clones of terminal deoxynucleotidyltransferase (TdT) from calf thymus and mouse lymphoma cDNA libraries. The libraries were constructed using the pcD vector...

Adherence targets of Vibrio parahaemolyticus in human small intestines.

In vitro adherence of type 1-fimbriated uropathogenic Escherichia coli to human ureteral mucosa.

Formalin-fixed human small intestinal mucosa with mucus coating, villi, and lymphoid follicle epithelium at the mucosal surface was used to test the adherence sites of clinically isolated (Kanagawa...

Fujita, K, Yamamoto, T, Yokota, T, Kitagawa, R

Type 1-fimbriated Escherichia coli isolated from patients with urinary tract infections adhered in vitro to the epithelial cell surface of an excised human ureter. The bacteria also adhered to a...

Isolation and characterization of a mouse interleukin cDNA clone that expresses B-cell stimulatory factor 1 activities and T-cell- and mast-cell-stimulating activities.

Lee, F, Yokota, T, Otsuka, T, Meyerson, P, Villaret, D, Coffman, R, ...

A cDNA sequence coding for a unique mouse interleukin that expresses B-cell-, T-cell, and mast-cell-stimulating activities has been isolated from a mouse helper T-cell cDNA library. The library,...

Activation of T cell-derived lymphokine genes in T cells and fibroblasts: effects of human T cell leukemia virus type I p40x protein and bovine papilloma virus encoded E2 protein.

Miyatake, S, Seiki, M, Malefijt, R D, Heike, T, Fujisawa, J, Takebe, Y, ...

The effects of p40x, a product of an human T cell leukemia virus type I, on the activation of lymphokine genes were examined. The mouse GM-CSF and IL-3 genes were activated by cotransfection with a...

Structural analysis of the mouse chromosomal gene encoding interleukin 4 which expresses B cell, T cell and mast cell stimulating activities.

Otsuka, T, Villaret, D, Yokota, T, Takebe, Y, Lee, F, Arai, N, ...

Based on homology with the mouse interleukin 4 (IL-4) cDNA that expresses B cell, T cell, and mast cell stimulating activities (Lee, F. et al., (1986) Proc. Natl. Acad. Sci. USA 83, 2061), we have...

Isolation and characterization of a mouse cDNA clone that expresses mast-cell growth-factor activity in monkey cells.

Yokota, T, Lee, F, Rennick, D, Hall, C, Arai, N, Mosmann, T, ...

A cDNA sequence coding for mouse mast-cell growth-factor (MCGF) has been cloned from a cDNA library prepared from mRNA derived from a concanavalin A-activated mouse T-cell clone. Cloning was achieved...

Molecular cloning of a novel human cDNA encoding a zinc finger protein that binds to the interleukin-3 promoter.

Koyano-Nakagawa, N, Nishida, J, Baldwin, D, Arai, K, Yokota, T

The CT/GC-rich region (-76 to -47) is one transcriptional regulatory region of the interleukin-3 (IL-3) gene which confers basic transcriptional activity and responds to trans-activation by human...

Reconstituted human granulocyte-macrophage colony-stimulating factor receptor transduces growth-promoting signals in mouse NIH 3T3 cells: comparison with signalling in BA/F3 pro-B cells.

Watanabe, S, Mui, A L, Muto, A, Chen, J X, Hayashida, K, Yokota, T, ...

Granulocyte-macrophage colony-stimulating factor (GM-CSF) plays a critical role in growth and differentiation of myeloid cells. We previously reconstituted high-affinity human GM-CSF receptor...

Isolation and characterization of a human interleukin cDNA clone, homologous to mouse B-cell stimulatory factor 1, that expresses B-cell- and T-cell-stimulating activities.

Yokota, T, Otsuka, T, Mosmann, T, Banchereau, J, DeFrance, T, Blanchard, D, ...

A cDNA sequence coding for a human interleukin has been isolated from a concanavalin A-activated human T-cell cDNA library based on homology with a mouse interleukin cDNA that expresses B-cell...

Isolation of cDNA for a human granulocyte-macrophage colony-stimulating factor by functional expression in mammalian cells.

Lee, F, Yokota, T, Otsuka, T, Gemmell, L, Larson, N, Luh, J, ...

A cDNA sequence coding for a human granulocyte-macrophage colony-stimulating factor has been isolated from cDNA libraries prepared from mRNA derived from concanavalin A-activated human T-cell clones....

ATL-derived factor (ADF), an IL-2 receptor/Tac inducer homologous to thioredoxin; possible involvement of dithiol-reduction in the IL-2 receptor induction.

Tagaya, Y, Maeda, Y, Mitsui, A, Kondo, N, Matsui, H, Hamuro, J, ...

Use of a cDNA expression vector for isolation of mouse interleukin 2 cDNA clones: expression of T-cell growth-factor activity after transfection of monkey cells.

Yokota, T, Arai, N, Lee, F, Rennick, D, Mosmann, T, Arai, K

A cDNA sequence coding for mouse interleukin 2 (IL-2) has been cloned from a cDNA library prepared from mRNA derived from a concanavalin A-activated mouse T-cell clone. The library was constructed by...

Structure of the chromosomal gene for murine interleukin 3.

Miyatake, S, Yokota, T, Lee, F, Arai, K

We have isolated the mouse interleukin 3 (IL-3) gene from a mouse sperm DNA library based on homology with the mouse mast-cell growth-factor (MCGF) cDNA sequence. The nucleotide sequence determined...

Suppression of apoptotic death in hematopoietic cells by signalling through the IL-3/GM-CSF receptors.

Kinoshita, T, Yokota, T, Arai, K, Miyajima, A

Interleukin 3 (IL-3) and granulocyte-macrophage colony stimulating factor (GM-CSF) exert their biological functions through acting on a specific receptor which consists of a ligand-specific alpha...

ATL-derived factor (ADF), an IL-2 receptor/Tac inducer homologous to thioredoxin; possible involvement of dithiol-reduction in the IL-2 receptor induction.

Tagaya, Y, Maeda, Y, Mitsui, A, Kondo, N, Matsui, H, Hamuro, J, ...

HTLV-I transformed T cells not only express a large number of interleukin-2 receptors [IL-2R/p55(Tac)], but also produce a factor named ATL-derived factor (ADF) that augments the expression of...

Increased adhesion of Chinese hamster ovary cells to substratum by cholera enterotoxin.

Nozawa, R, Kon, F, Yokota, T, Ohashi, M, Kuwahara, S

A new, simple assay method using Chinese hamster ovary cells was devised for cholera enterotoxin. The effect of the cholera toxin was measured by an increase in cell adhesion to the substratum. The...

Systemic reactive amyloidosis associated with Castleman's disease: serial changes of the concentrations of acute phase serum amyloid A and interleukin 6 in serum.

Ikeda, S, Chisuwa, H, Kawasaki, S, Ozawa, J, Hoshii, Y, Yokota, T, ...

A case is reported of a 21 year old woman who suffered from Castleman's disease and systemic reactive amyloidosis. The serum concentrations of serum amyloid A (SAA) and interleukin 6 (IL-6) were...

Structure of the chromosomal gene for granulocyte-macrophage colony stimulating factor: comparison of the mouse and human genes.

Miyatake, S, Otsuka, T, Yokota, T, Lee, F, Arai, K

A cDNA clone that expresses granulocyte-macrophage colony stimulating factor (GM-CSF) activity in COS-7 cells has been isolated from a pcD library prepared from mRNA derived from concanavalin...

STAT3 activation is sufficient to maintain an undifferentiated state of mouse embryonic stem cells.

Matsuda, T, Nakamura, T, Nakao, K, Arai, T, Katsuki, M, Heike, T, ...

Embryonic stem (ES) cells can be maintained in an undifferentiated state in the presence of leukemia inhibitory factor (LIF). LIF acts through a receptor complex composed of a low affinity LIF...

Depolarization of afferent terminals evoked by muscle stretch.

Muscle stretch and the presynaptic inhibition of the group Ia pathway to motoneurones.