Tadaatsu Akagi

Expression of thyroglobulin on follicular dendritic cells of thyroid mucosa-associated lymphoid tissue (MALT) lymphoma (2009)

Munemasa, Mitsuru, Yoshino, Tadashi, Kobayashi, Keita, Miyake, Takayoshi, Sakugawa, Sumie Takase, Mannami, Tomohiko, ...

Reportedly, thyroid mucosa-associated lymphoid tissue (MALT) lymphoma is closely associated with Hashimoto's thyroiditis. However, it remains unknown which antigen is closely associated with thyroid...

Spontaneous neoplastic transformation in vitro of mouse whole embryo cells and newborn hamster lung cells (2007)

Akagi, Tadaatsu

Three cell lines, spontaneously transformed in vitro, MWE-l and MWE-2 originating from C3Hf/Bi mouse whole embryos and NHLu-l originating from newborn syrian hamster lungs were obtained. These...

Human cell line (HGC-27) derived from the metastatic lymph node of gastric cancer (2007)

Akagi, Tadaatsu, Kimoto, Tetsuo

A cell line (HGC-27) was established by culture of the metastatic lymph node from a gastric cancer patient diagnosed histologically as undifferentiated carcinoma. HGC-27 cells were polygonal or short...

Characterization of xenoantiserum produced against B cell acute lymphoblastic leukemia cell line (2007)

Akagi, Tadaatsu, Sonobe, Hiroshi, Miyoshi, Isao, Yoshimoto, Shizuo

Antiserum was produced in white rabbit by intravenously injecting living cells of a B cell acute lymphoblastic leukemia (ALL) line (BALL-1). The reactivity of the antiserum against various lymphoid...

Application of polymerase chain reaction (PCR) to the microscopically identified cells on the slides: evaluation of specificity and sensitivity of single cell PCR. (2007)

Teramoto, Norihiro, Tonoyama, Yuji, Akagi, Tadaatsu, Sarker, Ashit Baran, Yoshino, Tadashi, Yamadori, Ichiro, ...

The sensitivity and specificity of single cell polymerase chain reaction (PCR) were studied. Its high sensitivity enabled detection of a single-copy gene, such as human T-lymphotropic virus type I...

Partial Purification and Chraracterization of Dendritic Cell Differentiation Factor (2007)

Miyatani, Katsuya, Takahashi, Kiyoshi, Yanai, Hiroyuki, Yoshino, Tadashi, Akagi, Tadaatsu

Previously, we reported that interleukin-2 (IL-2)-stimulated helper T cells produced an unknown soluble factor which induced dendritic cell-like differentiation in primary cultures of monocytic...

Malignant lymphoma induction in rabbits by oral inoculation of crude virus fraction prepared from Ts-B6 cells (cynomolgus B-lymphoblastoid cells harboring Epstein-Barr virus-related simian herpesvirus) (2007)

Chen, Hong Li, Hayashi, Kazuhiko, Koirala, Tirtha Raj, Ino, Hideo, Fuimoto, Koji, Yoshikawa, Yasuhiro, ...

Malignant lymphoma was induced in Japanese (JWY), New Zealand (NZY) and Dutch (DUY) white rabbits by oral spray of cell-free pellets of culture fluid (crude virus fraction) of Ts-B6 cells (cynomolgus...

Variable expression of Epstein-Barr virus latent membrane protein I in Reed-Sternberg cells of Hodgkin's disease. (2007)

Teramoto, Norihito, Cao, Liu, Kawasaki, Nobuhiro, Tonoyama, Yuji, Sarker, Ashit Baran, Yoshino, Tadashi, ...

Reed-Sternberg cells (RS cells) of Hodgkin's disease (HD) are frequently infected with Epstein-Barr virus (EBV) and express EBV-encoded nonpolyadenylated RNA transcripts (EBER)-1. EBV latency has...

Regulation of Interleukin-2 Receptor y Chain mRNA Expression in Human Monocytic Cell Line THP-1 (2007)

Yanai, Hiroyuki, Yoshino, Tadashi, Takahashi, Kiyoshi, Ninomiya, Yoshifumi, Akagi, Tadaatsu

Circulating hepatitis C virus (HCV) particles can be fractionated by means of differential flotation centrifugation. It is reported that in the bottom fraction HCV is in the form immune complexes,...

Detection of Epstein-Barr virus RNA and related antigens in non-neoplastic lymphoid lesions. (2007)

Tonoyama, Yuji, Teramoto, Norihiro, Sarker, Ashit Baran, Yoshino, Tadashi, Hayashi, Kazuhiko, Takahashi, Kiyoshi, ...

To elucidate the latent state and reactivation of Epstein-Barr virus (EBV) in non-neoplastic lymphoid lesions, we investigated 144 non-neoplastic lymphoid lesions by in situ hybridization (ISH) to...

Expression of a lymphocyte adhesion molecule (CD44) in malignant lymphomas: relevance to primary site, histological subtype and clinical stage. (2007)

Fujiwara, Kotaro, Yoshino, Tadashi, Miyake, Kenji, Ohara, Nobuya, Akagi, Tadaatsu

Lymphocyte adhesion molecules defined by anti-CD44 antibody (Hermes-3) may be involved in lymphocyte binding to high endothelial venules at sites where lymphocytes exist the blood. CD44 expression...

Distribution of Lectin Receptors in the Human Hyperplastic Tonsil: Histochemical and Flow Cytometric Analyses (2007)

Sarker, Ashit Baran, Akagi, Tadaatsu, Yoshino, Tadashi, Fujiwara, Kotaro, Nose, Soichiro

The distribution of lectin receptors in the human tonsil was studied using 16 biotinylated lectins. The avidin-biotin-peroxidase complex (ABC) method was used on frozen and paraffin-embedded tissue...

Kimura's disease: clinical, histological and immunohistochemical studies. (2007)

Motoi, Makoto, Wahid, Syariffudin, Horie, Yasushi, Akagi, Tadaatsu

In the present study, 14 cases of Kimura's disease were clinicopathologically studied. The disease occurred at ages ranging from 5 to 75 years. The average age was 37.8 years. Sexes were about...

Detection of oncogene rearrangements in human non-Hodgkin's lymphomas. (2007)

Kondo, Eisaku, Yoshino, Tadashi, Akagi, Tadaatsu, Hayashi, Kazuhiko, Takahashi, Kiyoshi

Southern blot hybridization was used to detect the rearrangement and amplification of five proto-oncogenes (bcl-2, bcl-1, c-myc, c-myb and c-Ha-ras) and one tumor suppressor gene (RB-1) in 55...

Establishment and characterization of a cell line, KaMi, from human lung large cell carcinoma. (2007)

Takata, Hiroshi, Yoshino, Tadashi, Hoshida, Yoshihiko, Takata, Ikuko, Akagi, Tadaatsu

A cell line of human lung large cell carcinoma (LCC) was established directly from the metastatic skin tumor tissue. The clinical course of the patient who carried this carcinoma was peculiar;...

A monoclonal antibody (OPT1) to T cells which is available for paraffin-embedded materials. (2007)

Mukuzono, Hiroshi, Yoshino, Tadashi, Akagi, Tadaatsu

A monoclonal antibody (MAb), OPT1, reactive with T cells in formalin-fixed, paraffin-embedded tissue sections, has been identified through immunization with activated T cells from peripheral blood...

Effect of a long-acting somatostatin analogue (SMS 201-995) on a growth hormone and thyroid stimulating hormone-producing pituitary tumor. (2007)

Hirasawa, Ryoto, Hashimoto, Hozo, Makino, Shinya, Suemaru, Shuso, Takao, Toshihiro, Ota, Zensuke, ...

A 46-year-old woman with acromegaly and hyperthyroidism due to a pituitary adenoma. She had high serum thyroid-stimulating hormone (TSH) levels and very high serum growth hormone (GH) levels....

Pre-elastofibroma-like colonic polyp: another cause of colonic polyp. (2007)

Hayashi, Kazuhiko, Ohtsuki, Yuji, Sonobe, Hiroshi, Iwata, Jun, Furihata, Matsuo, Hikita, Tomonori, ...

We present a case of pre-elastofibroma-like lesion, a kind of elastic-producing fibrous tumor. The small colonic polyp, which was found in a 49-year-old asymptomatic man in association with a large...

Comparison of monoclonal antibodies reactive with lymphocyte subsets in routinely fixed paraffin-embedded material: flow cytometric analyses, immunoperoxidase staining and influence of fixatives. (2007)

Yoshino, Tadashi, Hoshida, Yoshihiko, Murakami, Ichiro, Takahashi, Kiyoshi, Akagi, Tadaatsu

We have attempted to clarify the characteristics of monoclonal antibodies (MAbs) detecting lymphocyte subsets in fixed materials. We examined by means of flow cytometric technique influences of...

Ontogeny of S-100 protein-positive histiocytes and lymphocytes in the human fetal lymphoreticular system. (2007)

Akagi, Tadaatsu, Nose, Soichiro, Takahashi, Kiyoshi, Yoshino, Tadashi, Horie, Yasushi, Motoi, Makoto, ...

In the human lymphoreticular system, the alpha and beta subunits of S-100 protein are found in ordinary monocyte-macrophages and non-phagocytic histiocytes such as Langerhans cells and...

Immortalization of rat spleen and thymus T cells by human T-cell leukemia virus type I. (2007)

Akagi, Tadaatsu, Takata, Hiroshi, Yoshino, Tadashi, Teramoto, Norihiro, Yano, Shoki, Oka, Takashi

Co-cultivation of thymus and spleen cells of Fisher and Lewis rats with lethally irradiated MT-2 cells harboring human T-cell leukemia virus type I (HTLV-I) resulted in the establishment of lymphoid...

Metaplastic bone formation in a hyperplastic polyp of the stomach: a case report. (2007)

Ohtsuki, Yuji, Danbara, Yoshifumi, Takeda, Isao, Takahashi, Kiyoshi, Hayashi, Kazuhiko, Sonobe, Hiroshi, ...

Metaplastic bony tissue along with hyperplastic mucosal epithelium showing no atypism was detected in biopsy materials from a Yamada type I gastric polyp. The tissue was metaplastic woven bone...

Transformation of human fetal thymus and spleen lymphocytes by human t-cell leukemia virus type Ι (2007)

Akagi, Tadaatsu, Ohtsuki, Yuji, Shiraishi, Yukimasa, Miyoshi, Isao

Co-cultivation of human thymus and spleen lymphocytes, which were obtained from 26-week and 27-week fetuses, with a lethally-irradiated human cord T-cell line harboring human T-cell leukemia virus...

Detection of HLA-D-region-associated antigens on the surface of adult T-cell leukemia virus particles by immunoelectron microscopy. (2007)

Ohtsuki, Yuji, Akagi, Tadaatsu, Takeda, Isao, Miyoshi, Isao

To search for lymphocyte marker antigens on the surface of human T-cell leukemia virus (HTLV), an immunoelectron microscopic study was performed on a HTLV-producing human T-cell line, MT-2, using...

Antibody and Cytokine Responses in Helicobacter pylori-Infected Various Mouse Strains (2007)

Dey, Ashoka, Yokota, Kenji, Kobayashi, Keita, Oguma, Keiji, Hirai, Yoshikazu, Akagi, Tadaatsu

Helicobacter pylori (H. pylori) infection in the stomach is etiologically closely associated with chronic active gastritis, peptic ulcer, gastric cancer and gastric mucosa-associated lymphoid tissue...

Expression and intracellular localization of FKHRL1 in mammary gland neoplasms. (2006)

Jin, Gui-Shan, Kondo, Eisaku, Miyake, Takayoshi, Shibata, Masao, Takashima, Takako, Liu, Yi-Xuan, ...

FKHRL1 (FOXO3a), a member of the Forkhead family of genes, has been considered to be involved in the development of breast tumors; however, the in vivo expression and activation status of FKHRL1 in...

Recent advances in DNA microarrays. (2006)

Nakanishi, Tohru, Oka, Takashi, Akagi, Tadaatsu

The structure of the human genome is almost completely elucidated and the life sciences will now aim for a general and integrated study of gene expressions and the functional elucidation of proteins....

Characterization of epstein-barr virus-infected mantle cell lymphoma lines. (2006)

Jin, Zaishun, Teramoto, Norihiro, Yoshino, Tadashi, Takada, Kenzo, Oka, Takashi, Hayashi, Kazuhiko, ...

It has been reported that Epstein-Barr virus (EBV) resides in resting B cells in vivo. However, an ideal in vitro system for studying EBV latent infection in vivo has not yet been established. In...

A new experimental system for irradiating tumors in mice using a linear accelerator under specific pathogen-free conditions. (2006)

Kuroda, Masahiro, Inamura, Keiji, Tahara, Seiji, Kurabayashi, Yuzuru, Akagi, Tadaatsu, Asaumi, Junichi, ...

We developed a reliable system for the irradiation of xenografted tumors in mice which allows for accurate local irradiation under specific pathogen-free conditions. The system presented here...

Heterogeneity of Dendritic Cells in Human Superficial Lymph Node : In Vitro Maturation of Immature Dendritic Cells into Mature or Activated Interdigitating Reticulum Cells

Takahashi, Kiyoshi, Asagoe, Kenji, Zaishun, Jin, Yanai, Hiroyuki, Yoshino, Tadashi, Hayashi, Kazuhiko, ...

A two-color immunofluorescent analysis indicated that dendritic cells (DCs) in the human axillar lymph nodes (ie, lymph nodal DCs (LnDCs)) can be classified into three subsets. The first subset...

Reduction of Hematopoietic Cell-Specific Tyrosine Phosphatase SHP-1 Gene Expression in Natural Killer Cell Lymphoma and Various Types of Lymphomas/Leukemias : Combination Analysis with cDNA Expression Array and Tissue Microarray

Oka, Takashi, Yoshino, Tadashi, Hayashi, Kazuhiko, Ohara, Nobuya, Nakanishi, Tohru, Yamaai, Yuichiro, ...

To investigate the lymphomagenesis of NK/T lymphoma, we comprehensively and systematically analyzed the expression pattern of the human NK/T cell line (NK-YS) genome by cDNA expression array and...

An Animal Model for Human EBV-Associated Hemophagocytic Syndrome : Herpesvirus Papio Frequently Induces Fatal Lymphoproliferative Disorders with Hemophagocytic Syndrome in Rabbits

Hayashi, Kazuhiko, Ohara, Nobuya, Teramoto, Norihiro, Onoda, Sachiyo, Chen, Hong-Li, Oka, Takashi, ...

Epstein-Barr virus-associated hemophagocytic syndrome (EBV-AHS) is often associated with fatal infectious mononucleosis. However, the animal model for EBV-AHS has not been developed. We reported the...