Kikuchi, Yutaka, Irie, Masachike, Ikebuchi, Hidehary, Sawada, Jun-ichi, Terao, Tadao, Nakayama, Shigeru, ...
In order to determine the epitope of metallothionein (MT) to a murine monoclonal antibody (MT 189– 14– 7) which had been produced by immunization with rat MT 2 (Kikuchi et al. (1988) Mol....
SUZUKI, Kazuhiro, SUZUKI, Sumiko, TERAO, Tadao, OSAWA, Toshiaki
A cyclic AMP-binding protein of human erythrocyte membranes was solubilized with 0.3% Triton X-100 in 10 mM Tris-HCl (pH 7.4) at 4�C, and purified by DEAE-cellulose column chromatography and...
SUZUKI, Kazuhiro, TERAO, Tadao, OSAWA, Toshiaki
Protein kinase [EC 2.7.1.37] of human erythrocyte membranes was solubilized with 0.5 M NaCl in 5 mM phosphate buffer, pH 6.7 at 4°C and purified on a CM-Sephadex C-50 column, followed by affinity...
KATAGIRI, Takuya, ADACHI, Isao, TERAO, Tadao, OSAWA, Toshiaki
The existence of specific binding proteins for α-casein, a well-known potent chemoattractant, on guinea pig peritoneal macrophages was demonstrated. Binding of 3H-α-casein to macrophages was found...
Covalently Cross-linked Monovalent, Divalent, and Tetravalent Derivatives of Concanavalin A (1979)
BEPPU, Masatoshi, TERAO, Tadao, OSAWA, Toshiaki
Dimeric succinyl-concanavalin A was cross-linked with ethylenediamine using 1-ethyl-3-(3- dimethylaminopropyl)carbodiiniide a condensing agent. Thus, a divalent dimer and a tetravalent tetramer...
Membrane Receptors of Mouse Lymphocytes for Various Lectins (1977)
IWATA, Makoto, IDE, Hayao, TERAO, Tadao, OSAWA, Toshiaki
The major glycoproteins which bind various lectins (Bauhinia purpurea, Lens culinaris, Pisum sativum, Ricinus communis, and wheat germ hemagglutinins) were isolated from splenic lymphocytes of...
KIYOHARA, Takao, TERAO, Tadao, SHIOIRI-NAKANO, Kohei, OSAWA, Toshiaki
Three β-N-acetylhexosaminidases [EC 3. 2. 1. 52] and one β-galactosidase [EC 3. 2. 1. 23] were purified from the culture filtrate of Streptococcus 6646 group K by a combination of column...
Preparation of Monovalent Succinyl-concanavalin A and Its Mitogenic Activity (1976)
BEPPU, Masatoshi, TERAO, Tadao, OSAWA, Toshiaki
Monovalent succinyl-concanavalin A was prepared by photoaffinity labeling of succinyl-concanavalin A with p-azidophenyl α-D-mannopyranoside. This concanavalin A derivative showed a weak but definite...
Activation of Mouse Splenic Lymphocyte Guanylate Cyclase by Calcium Ion (1976)
KATAGIRI, Takuya, TERAO, Tadao, OSAWA, Toshiaki
The guanosine 3', 5'-cyclic monophosphate (cGMP) level in the mouse splenic lymphocytes was increased about 2- to 3-fold by concanavalin A. This increase was completely dependent on the presence of...
BEPPU, Masatoshi, TERAO, Tadao, OSAWA, Toshiaki
Concanavalin A (Con A) was labeled with p-azidophenyl α-D-mannopyranoside under ultraviolet irradiation and the reaction products were separated by affinity chromatography on Sephadex G-100 at pH 5....