The WASP and WAVE family proteins (2009)
Kurisu, Shusaku, Takenawa, Tadaomi
Summary All eukaryotic cells need to reorganize their actin cytoskeleton to change shape, divide, move, and take up nutrients for survival. The Wiskott-Aldrich syndrome protein (WASP) and WASP-family...
Effect of WAVE2 Phosphorylation on Activation of the Arp2/3 Complex (2007)
Nakanishi, Osamu, Suetsugu, Shiro, Yamazaki, Daisuke, Takenawa, Tadaomi
Members of the family of WASP-family Verprolin homologous proteins (WAVEs) activate the Arp2/3 complex to induce actin polymerization. The WAVE family comprises three proteins, namely, WAVE1, WAVE2...
Effect of WAVE2 phosphorylation on activation of the Arp2/3 complex (2007)
Nakanishi, Osamu, Suetsugu, Shiro, Yamazaki, Daisuke, Takenawa, Tadaomi
Members of the family of WASP-family Verprolin homologous proteins (WAVEs) activate the Arp2/3 complex to induce actin polymerization. The WAVE family comprises three proteins, namely, WAVE1, WAVE2,...
Effect of WAVE2 phosphorylation on activation of the Arp2/3 complex (2007)
Nakanishi, Osamu, Suetsugu, Shiro, Yamazaki, Daisuke, Takenawa, Tadaomi
Members of the family of WASP-family Verprolin homologous proteins (WAVEs) activate the Arp2/3 complex to induce actin polymerization. The WAVE family comprises three proteins, namely, WAVE1, WAVE2,...
Thin Layer Chromatography-Blotting, a Novel Method for the Detection of Phosphoinositides (2006)
Furutani, Masahiro, Itoh, Toshiki, Ijuin, Takeshi, Tsujita, Kazuya, Takenawa, Tadaomi
Phosphoinositides are believed to be involved in fundamental cellular events such as signal transduction and vesicular trafficking. Aberrant metabolisms of this lipid, caused by mutations in...
Akutagawa, Atsushi, Fukami, Kiyoko, Banno, Yoshiko, Takenawa, Tadaomi, Kannagi, Reiji, Yokoyama, Yukihiro, ...
Phospholipase Cδ4 (PLC δ4) gene has been cloned from the cDNA library of regenerating rat liver. Using PLC δ4 gene–disrupted mice (PLC δ4−/−), we studied a role of PLC δ4 during liver...
Akutagawa, Atsushi, Fukami, Kiyoko, Banno, Yoshiko, Takenawa, Tadaomi, Kannagi, Reiji, Yokoyama, Yukihiro, ...
Phospholipase Cδ4 (PLC δ4) gene has been cloned from the cDNA library of regenerating rat liver. Using PLC δ4 gene-disrupted mice (PLC δ4-/-), we studied a role of PLC δ4 during liver...
Phospholipase C-�1 and-�3 Are Essential in the Trophoblast for Placental Development (2005)
Yoshikazu Nakamura, Yoshio Hamada, Takashi Fujiwara, Hiroko Enomoto, Takeshi Hiroe, Satoshi Tanaka, ...
Phosphoinositide-specific phospholipase C (PLC) is a key enzyme in phosphoinositide turnover and is involved in a variety of physiological functions. We analyzed PLC�1 knockout mice and found that...
WAVE3 Functions as a Negative Regulator of LDOC1 (2005)
Mizutani, Kiyohito, Koike, Daisuke, Suetsugu, Shiro, Takenawa, Tadaomi
WAVE3 belongs to the Wiskott-Aldrich syndrome protein family that mediates actin reorganization through activation of the Arp2/3 complex. However, the physiological function of WAVE3 is poorly...
Regulation of Actin Dynamics by WASP Family Proteins (2003)
Miki, Hiroaki, Takenawa, Tadaomi
Rapid reorganization of the actin cytoskeleton underlies morphological changes and motility of cells. WASP family proteins have received a great deal of attention as the signal-regulated molecular...
Influence of age on the signal transduction of T cells in mice (1993)
Utsuyama, Masanori, Varga, Zsuzsa, Fukami, Kiyoko, Homma, Yoshimi, Takenawa, Tadaomi, Hirokawa, Katsuiku, ...
A 5- to 10-fold decline was observed in the proliferative activity of T cells stimulated with anti- CD3 mAb between young and old mice. However, the number of CD3 molecules on the T cell surface was...
SUZUKI, Reiko, TAKENAWA, Tadaomi
otropin releasing hormone (TRH) caused significant breakdown of phosphatidylinositol 4, 5-bisphosphate (PIP2) in GH3 cells, but vasoactive intestinal peptide (VIP) did not. However, VIP enhanced the...
TAKENAWA, Tadaomi, MASAKI, Tomoh, GOTO, Katsutoshi
Surgical denervation of rat vas deferens causes supersensitivity in that the tissue sensitivity and the maximum response to a variety of agonists increase. To understand the molecular mechanism of...
Effect of Unsaturated Fatty Acids and Ca2+ on Phosphatidylinositol Synthesis and Breakdown (1982)
TAKENAWA, Tadaomi, NAGAI, Yoshitaka
CDP-diglyceride : inositol transferase was inhibited by unsaturated fatty acids. The inhibitory activity decreased in the following order: arachidonic acid≫linolenic acid≫linoleic acid≫oleic...
Yamaguchi, Hideki, Miki, Hiroaki, Suetsugu, Shiro, Ma, Le, Kirschner, Marc W., Takenawa, Tadaomi
All WASP family proteins share a common C terminus that consists of the verprolin homology domain (V), cofilin homology domain (C), and acidic region (A), through which they activate Arp2/3...
He, Hong, Watanabe, Takeshi, Zhan, Xi, Huang, Cai, Schuuring, Ed, Fukami, Kiyoko, ...
Oncogenic Ras mutants such as v-Ha-Ras cause a rapid rearrangement of actin cytoskeleton during malignant transformation of fibroblasts or epithelial cells. Both PI-3 kinase and Rac are required for...
SKIP Negatively Regulates Insulin-Induced GLUT4 Translocation and Membrane Ruffle Formation
Ijuin, Takeshi, Takenawa, Tadaomi
Skeletal muscle and kidney enriched inositol phosphatase (SKIP) is an inositol polyphosphate 5-phosphatase that hydrolyzes phosphatidylinositol 3,4,5-trisphosphate [PI(3,4,5)P3] to downregulate...
Phospholipase Cδ1 is required for skin stem cell lineage commitment
Nakamura, Yoshikazu, Fukami, Kiyoko, Yu, Haiyan, Takenaka, Kei, Kataoka, Yuki, Shirakata, Yuji, ...
Phosphoinositide-specific phospholipase C (PLC) is a key enzyme in phosphoinositide turnover and is involved in a variety of physiological functions. Here we report that PLCδ1-deficient mice...
Takenaka, Kei, Fukami, Kiyoko, Otsuki, Makiko, Nakamura, Yoshikazu, Kataoka, Yuki, Wada, Mika, ...
Phospholipase C (PLC) plays important roles in phosphoinositide turnover by regulating the calcium-protein kinase C signaling pathway. PLC-L2 is a novel PLC-like protein which lacks PLC activity,...
Regulation of actin cytoskeleton by mDab1 through N-WASP and ubiquitination of mDab1
Suetsugu, Shiro, Tezuka, Tohru, Morimura, Toshifumi, Hattori, Mitsuharu, Mikoshiba, Katsuhiko, Yamamoto, Tadashi, ...
Migration of cells is critical to development of the central nervous system. Reelin, which was identified from the reeler mutant mice having a defect in the multilamellar structure of the brain, is...
Park, Sun Joo, Suetsugu, Shiro, Takenawa, Tadaomi
Neural Wiskott–Aldrich syndrome protein (N-WASP) regulates reorganization of the actin cytoskeleton through activation of the Arp2/3 complex. Here, we show that heat shock protein 90 (HSP90)...
Takenawa, Tadaomi, Homma, Yoshimi, Nagai, Yoshitaka
The effects of calmodulin antagonists on the secretion of lysosomal enzyme and lipid metabolism in guinea-pig peritoneal macrophages were studied. Calmodulin antagonists, such as trifluoperazine,...
CRMP-2 Is Involved in Kinesin-1-Dependent Transport of the Sra-1/WAVE1 Complex and Axon Formation†
Kawano, Yoji, Yoshimura, Takeshi, Tsuboi, Daisuke, Kawabata, Saeko, Kaneko-Kawano, Takako, Shirataki, Hiromichi, ...
A neuron has two types of highly polarized cell processes, the single axon and multiple dendrites. One of the fundamental questions of neurobiology is how neurons acquire such specific and polarized...
Phospholipase C-δ1 and -δ3 Are Essential in the Trophoblast for Placental Development
Nakamura, Yoshikazu, Hamada, Yoshio, Fujiwara, Takashi, Enomoto, Hiroko, Hiroe, Takeshi, Tanaka, Satoshi, ...
Phosphoinositide-specific phospholipase C (PLC) is a key enzyme in phosphoinositide turnover and is involved in a variety of physiological functions. We analyzed PLCδ1 knockout mice and found that...
Yamaguchi, Hideki, Miki, Hiroaki, Suetsugu, Shiro, Ma, Le, Kirschner, Marc W., Takenawa, Tadaomi
All WASP family proteins share a common C terminus that consists of the verprolin homology domain (V), cofilin homology domain (C), and acidic region (A), through which they activate Arp2/3...
He, Hong, Watanabe, Takeshi, Zhan, Xi, Huang, Cai, Schuuring, Ed, Fukami, Kiyoko, ...
Oncogenic Ras mutants such as v-Ha-Ras cause a rapid rearrangement of actin cytoskeleton during malignant transformation of fibroblasts or epithelial cells. Both PI-3 kinase and Rac are required for...
SKIP Negatively Regulates Insulin-Induced GLUT4 Translocation and Membrane Ruffle Formation
Ijuin, Takeshi, Takenawa, Tadaomi
Skeletal muscle and kidney enriched inositol phosphatase (SKIP) is an inositol polyphosphate 5-phosphatase that hydrolyzes phosphatidylinositol 3,4,5-trisphosphate [PI(3,4,5)P3] to downregulate...
Phospholipase Cδ1 is required for skin stem cell lineage commitment
Nakamura, Yoshikazu, Fukami, Kiyoko, Yu, Haiyan, Takenaka, Kei, Kataoka, Yuki, Shirakata, Yuji, ...
Phosphoinositide-specific phospholipase C (PLC) is a key enzyme in phosphoinositide turnover and is involved in a variety of physiological functions. Here we report that PLCδ1-deficient mice...
Takenaka, Kei, Fukami, Kiyoko, Otsuki, Makiko, Nakamura, Yoshikazu, Kataoka, Yuki, Wada, Mika, ...
Phospholipase C (PLC) plays important roles in phosphoinositide turnover by regulating the calcium-protein kinase C signaling pathway. PLC-L2 is a novel PLC-like protein which lacks PLC activity,...
Regulation of actin cytoskeleton by mDab1 through N-WASP and ubiquitination of mDab1
Suetsugu, Shiro, Tezuka, Tohru, Morimura, Toshifumi, Hattori, Mitsuharu, Mikoshiba, Katsuhiko, Yamamoto, Tadashi, ...
Migration of cells is critical to development of the central nervous system. Reelin, which was identified from the reeler mutant mice having a defect in the multilamellar structure of the brain, is...
Park, Sun Joo, Suetsugu, Shiro, Takenawa, Tadaomi
Neural Wiskott–Aldrich syndrome protein (N-WASP) regulates reorganization of the actin cytoskeleton through activation of the Arp2/3 complex. Here, we show that heat shock protein 90 (HSP90)...
Takenawa, Tadaomi, Homma, Yoshimi, Nagai, Yoshitaka
The effects of calmodulin antagonists on the secretion of lysosomal enzyme and lipid metabolism in guinea-pig peritoneal macrophages were studied. Calmodulin antagonists, such as trifluoperazine,...
CRMP-2 Is Involved in Kinesin-1-Dependent Transport of the Sra-1/WAVE1 Complex and Axon Formation†
Kawano, Yoji, Yoshimura, Takeshi, Tsuboi, Daisuke, Kawabata, Saeko, Kaneko-Kawano, Takako, Shirataki, Hiromichi, ...
A neuron has two types of highly polarized cell processes, the single axon and multiple dendrites. One of the fundamental questions of neurobiology is how neurons acquire such specific and polarized...
Phospholipase C-δ1 and -δ3 Are Essential in the Trophoblast for Placental Development
Nakamura, Yoshikazu, Hamada, Yoshio, Fujiwara, Takashi, Enomoto, Hiroko, Hiroe, Takeshi, Tanaka, Satoshi, ...
Phosphoinositide-specific phospholipase C (PLC) is a key enzyme in phosphoinositide turnover and is involved in a variety of physiological functions. We analyzed PLCδ1 knockout mice and found that...
Tsujita, Kazuya, Suetsugu, Shiro, Sasaki, Nobunari, Furutani, Masahiro, Oikawa, Tsukasa, Takenawa, Tadaomi
The conserved FER-CIP4 homology (FCH) domain is found in the pombe Cdc15 homology (PCH) protein family members, including formin-binding protein 17 (FBP17). However, the amino acid sequence homology...
Optimization of WAVE2 complex–induced actin polymerization by membrane-bound IRSp53, PIP3, and Rac
Suetsugu, Shiro, Kurisu, Shusaku, Oikawa, Tsukasa, Yamazaki, Daisuke, Oda, Atsushi, Takenawa, Tadaomi
WAVE2 activates the actin-related protein (Arp) 2/3 complex for Rac-induced actin polymerization during lamellipodium formation and exists as a large WAVE2 protein complex with Sra1/PIR121, Nap1,...
Yokoyama, Takashi, Takano, Kazunori, Yoshida, Akira, Katada, Fumiko, Sun, Peng, Takenawa, Tadaomi, ...
Ras activates Raf, leading to the extracellular-regulated kinase (ERK)–mitogen-activated protein kinase pathway, which is involved in a variety of cellular, physiological, and pathological...
Yanagida-Asanuma, Etsuko, Asanuma, Katsuhiko, Kim, Kwanghee, Donnelly, Mary, Young Choi, Hoon, Hyung Chang, Jae, ...
The actin-based foot processes of kidney podocytes and the interposed slit diaphragm form the final barrier to proteinuria. Mutations affecting several podocyte proteins cause disruption of the...
Yamaguchi, Hideki, Lorenz, Mike, Kempiak, Stephan, Sarmiento, Corina, Coniglio, Salvatore, Symons, Marc, ...
Invadopodia are actin-rich membrane protrusions with a matrix degradation activity formed by invasive cancer cells. We have studied the molecular mechanisms of invadopodium formation in metastatic...
Rho Family Gtpase Cdc42 Is Essential for the Actin-Based Motility of Shigella in Mammalian Cells
Suzuki, Toshihiko, Mimuro, Hitomi, Miki, Hiroaki, Takenawa, Tadaomi, Sasaki, Takuya, Nakanishi, Hiroyuki, ...
Shigella, the causative agent of bacillary dysentery, is capable of directing its movement within host cells by exploiting actin dynamics. The VirG protein expressed at one pole of the bacterium can...
Phospholipase Cδ4 is required for Ca2+ mobilization essential for acrosome reaction in sperm
Fukami, Kiyoko, Yoshida, Manabu, Inoue, Takafumi, Kurokawa, Manabu, Fissore, Rafael A., Yoshida, Nobuaki, ...
Zona pellucida (ZP)–induced acrosome reaction in sperm is a required step for mammalian fertilization. However, the precise mechanism of the acrosome reaction remains unclear. We previously...
Profilin Enhances Cdc42-Induced Nucleation of Actin Polymerization
Yang, Changsong, Huang, Minzhou, DeBiasio, John, Pring, Martin, Joyce, Michael, Miki, Hiroaki, ...
We find that profilin contributes in several ways to Cdc42-induced nucleation of actin filaments in high speed supernatant of lysed neutrophils. Depletion of profilin inhibited Cdc42-induced...
Fukuoka, Maiko, Suetsugu, Shiro, Miki, Hiroaki, Fukami, Kiyoko, Endo, Takeshi, Takenawa, Tadaomi
We identified a novel adaptor protein that contains a Src homology (SH)3 domain, SH3 binding proline-rich sequences, and a leucine zipper-like motif and termed this protein WASP interacting SH3...
Sequential signals toward podosome formation in NIH-src cells
Oikawa, Tsukasa, Itoh, Toshiki, Takenawa, Tadaomi
Podosomes (also termed invadopodia in cancer cells) are actin-rich adhesion structures with matrix degradation activity that develop in various cell types. Despite their significant physiological...
Increased Insulin Action in SKIP Heterozygous Knockout Mice▿ †
Ijuin, Takeshi, Yu, Y. Eugene, Mizutani, Kiyohito, Pao, Annie, Tateya, Sanshiro, Tamori, Yoshikazu, ...
Insulin controls glucose homeostasis and lipid metabolism, and insulin impairment plays a critical role in the pathogenesis of diabetes mellitus. Human skeletal muscle and kidney enriched inositol...
The WASP and WAVE family proteins
Kurisu, Shusaku, Takenawa, Tadaomi
A review of the cytoskeleton-organizing WASP and WAVE family proteins.