Retrospective Study on Home Care for Patients with Hematologic Malignancies (2009)
Kodama, Yuko, Takita, Morihito, Kawagoe, Shohei, Hirahara, Satoshi, Kimura, Yukihiro, Onozawa, Shigeru, ...
Objective There is a lack of sufficient information on the employment of home care for the treatment of hematologic malignancies. Methods We provided home care to 580 patients from 1 January through...
Agawa, Yasuo, Sarhan, Moustafa, Kageyama, Yuji, Akagi, Kazutaka, Takai, Masayoshi, Hashiyama, Kazuya, ...
Regulatory mechanisms controlling the timing of developmental events are crucial for proper development to occur. ftz-fl is expressed in a temporally regulated manner following pulses of ecdysteroid...
Ando, Kozue, Hirao, Satoshi, Kabe, Yasuaki, Ogura, Yuji, Sato, Iwao, Yamaguchi, Yuki, ...
APE1/Ref-1 is thought to be a multifunctional protein involved in reduction–oxidation (redox) regulation and base excision DNA repair, and is required for early embryonic development in mice....
Yamamoto, Keisuke, Suzuki, Natsumi, Wada, Tadashi, Okada, Tetsuya, Yoshida, Hiderou, Kaufman, Randal J., ...
Quality control of proteins in the endoplasmic reticulum (ER) is achieved by two mechanisms, the productive folding mechanism, which is assisted by a number of ER-localized molecular chaperones and...
Plasma Membrane-associated Sialidase as a Crucial Regulator of Transmembrane Signalling (2008)
Miyagi, Taeko, Wada, Tadashi, Yamaguchi, Kazunori, Hata, Keiko, Shiozaki, Kazuhiro
Mammalian sialidases, glycosidases responsible for the removal of sialic acids from glycoproteins and glycolipids, has been implicated to participate in many biological processes as well as in...
Zhu, Wenyan, Wada, Tadashi, Okabe, Sachiko, Taneda, Takuya, Yamaguchi, Yuki, Handa, Hiroshi
The transcription elongation factor 5,6-dichloro-1-β-d-ribofuranosylbenzimidazole (DRB) sensitivity-inducing factor (DSIF) regulates RNA polymerase II (RNAPII) processivity by promoting, in concert...
Mutational Analysis of the Carboxyl-terminal Region of the SV40 Major Capsid Protein VP1 (2007)
Yokoyama, Naoki, Kawano, Masa-aki, Tsukamoto, Hiroko, Enomoto, Teruya, Inoue, Takamasa, Takahashi, Ryou-u, ...
Virus-like particles (VLPs), a promising next-generation drug delivery vehicle, can be formed in vitro using a recombinant viral capsid protein VP1 from SV40. Seventy-two VP1 pentamers interconnect...
Mutational analysis of the carboxyl-terminal region of the SV40 major capsid protein VP1 (2007)
Yokoyama, Naoki, Kawano, Masa-aki, Tsukamoto, Hiroko, Enomoto, Teruya, Inoue, Takamasa, Takahashi, Ryou-u, ...
Virus-like particles (VLPs), a promising next-generation drug delivery vehicle, can be formed in vitro using a recombinant viral capsid proteinVP1 from SV40. Seventy-two VP1 pentamers interconnect to...
Mutational analysis of the carboxyl-terminal region of the SV40 major capsid protein VP1 (2007)
Yokoyama, Naoki, Kawano, Masa-aki, Tsukamoto, Hiroko, Enomoto, Teruya, Inoue, Takamasa, Takahashi, Ryou-u, ...
Virus-like particles (VLPs), a promising next-generation drug delivery vehicle, can be formed in vitro using a recombinant viral capsid proteinVP1 from SV40. Seventy-two VP1 pentamers interconnect to...
Kubo, Tomohiro, Wada, Tadashi, Yamaguchi, Yuki, Shimizu, Akira, Handa, Hiroshi
Alternative polyadenylation leads to mRNAs with variable 3′ ends. Since a 3′-untranslated region (3′-UTR) often contains cis elements that impact stability or localization of mRNA or...
A Rapid Purification Method for Human RNA Polymerase II by Two-Step Affinity Chromatography (2003)
Hasegawa, Jun, Endou, Masaki, Narita, Takashi, Yamada, Tomoko, Yamaguchi, Yuki, Wada, Tadashi, ...
The molecular dissection of transcription mechanisms is greatly facilitated by constructing and manipulating defined transcription systems in vitro. This approach requires highly purified...
Yamaguchi, Yuki, Narita, Takashi, Inukai, Naoto, Wada, Tadashi, Handa, Hiroshi
Suppressor of Ty (SPT) genes were originally identified through a genetic screen for mutations in the yeast Saccharomyces cerevisiae that restore gene expression disrupted by the insertion of the...
Wada, Tadashi, Takagi, Toshiyuki, Yamaguchi, Yuki, Ferdous, Anwarul, Imai, Takeshi, Hirose, Susumu, ...
Hata, Keiko, Wada, Tadashi, Hasegawa, Akira, Kiso, Makoto, Miyagi, Taeko
A membrane-associated ganglioside-hydrolyzing sialidase was purified to apparent homogeneity from bovine brain. The enzyme was solubilized with Triton X-100 plus sodium cholate from the particulate...
Shimada, Hiroshi, Wada, Tadashi, Handa, Hiroshi, Ohta, Hiroyuki, Mizoguchi, Hiroshi, Nishimura, Kohji, ...
In the purple nonsulfur photosynthetic bacterium Rhodobacter sphaeroides the synthesis of components of the photosystem is regulated in response to oxygen tension and light intensity. We have...
Hashimoto, Toshihide, Morohashi, Ken-ichirou, Takayama, Koichi, Honda, Shin-Ichiro, Wada, Tadashi, Handa, Hiroshi, ...
We previously reported the presence of six different cis.-acting elements (Ad1 to Ad6) in the promoter region of the bovine CYP11B gene. Although the Ad1 site (TGACGTGA) was similar to a palindromic...
Kawaguchi, Haruma, Asai, Akira, Ohtsuka, Yasuji, Watanabe, Hajime, Wada, Tadashi, Handa, Hiroshi
A Simple method with the use of affinity latex particles has been developed for the fast and efficient purification of sequence-specific DNA-binding proteins on the basis of their ability to...
Tat-SF1 Protein Associates with RAP30 and Human SPT5 Proteins
Kim, Jae B., Yamaguchi, Yuki, Wada, Tadashi, Handa, Hiroshi, Sharp, Phillip A.
The potent transactivator Tat recognizes the transactivation response RNA element (TAR) of human immunodeficiency virus type 1 and stimulates the processivity of elongation of RNA polymerase (Pol) II...
Kakugawa, Yoichiro, Wada, Tadashi, Yamaguchi, Kazunori, Yamanami, Hideaki, Ouchi, Kiyoaki, Sato, Ikuro, ...
Human plasma membrane-associated sialidase (Neu3) is unique in specifically hydrolyzing gangliosides, thought to participate in cell differentiation and transmembrane signaling, thereby playing...
Yamaguchi, Yuki, Inukai, Naoto, Narita, Takashi, Wada, Tadashi, Handa, Hiroshi
Negative elongation factor (NELF) is a human transcription factor complex that cooperates with DRB sensitivity-inducing factor (DSIF)/hSpt4-hSpt5 to repress elongation by RNA polymerase II (RNAPII)....
Narita, Takashi, Yamaguchi, Yuki, Yano, Keiichi, Sugimoto, Seiji, Chanarat, Sittinan, Wada, Tadashi, ...
The multisubunit transcription elongation factor NELF (for negative elongation factor) acts together with DRB (5,6-dichloro-1-β-d-ribofuranosylbenzimidazole) sensitivity-inducing factor (DSIF)/human...
Wada, Tadashi, Takagi, Toshiyuki, Yamaguchi, Yuki, Ferdous, Anwarul, Imai, Takeshi, Hirose, Susumu, ...
We report the identification of a transcription elongation factor from HeLa cell nuclear extracts that causes pausing of RNA polymerase II (Pol II) in conjunction with the transcription inhibitor...
Hartzog, Grant A., Wada, Tadashi, Handa, Hiroshi, Winston, Fred
Previous characterization of the Saccharomyces cerevisiae Spt4, Spt5, and Spt6 proteins suggested that these proteins act as transcription factors that modify chromatin structure. In this work, we...
Human Spt6 Stimulates Transcription Elongation by RNA Polymerase II In Vitro
Endoh, Masaki, Zhu, Wenyan, Hasegawa, Jun, Watanabe, Hajime, Kim, Dong-Ki, Aida, Masatoshi, ...
Recent studies have suggested that Spt6 participates in the regulation of transcription by RNA polymerase II (RNAPII). However, its underlying mechanism remains largely unknown. One possibility,...
Bromodomain Factor 1 (Bdf1) Is Phosphorylated by Protein Kinase CK2
Sawa, Chika, Nedea, Eduard, Krogan, Nevan, Wada, Tadashi, Handa, Hiroshi, Greenblatt, Jack, ...
Bromodomain factor 1 (Bdf1) associates with Saccharomyces cerevisiae TFIID and corresponds to the C-terminal half of higher eukaryotic TAF1. It also associates with the SWR-C complex, which is...
Mandal, Subhrangsu S., Chu, Chun, Wada, Tadashi, Handa, Hiroshi, Shatkin, Aaron J., Reinberg, Danny
Capping of the 5′ ends of nascent RNA polymerase II transcripts is the first pre-mRNA processing event in all eukaryotic cells. Capping enzyme (CE) is recruited to transcription complexes soon...
Kabe, Yasuaki, Yamada, Joe, Uga, Hitoshi, Yamaguchi, Yuki, Wada, Tadashi, Handa, Hiroshi
Osteoclast differentiation factor (ODF)/receptor activator of NF-κB ligand is essential for inducing the differentiation of mature osteoclasts. We find that nuclear factor Y (NF-Y) binds to the...
Evidence for mitochondrial localization of a novel human sialidase (NEU4)
Yamaguchi, Kazunori, Hata, Keiko, Koseki, Koichi, Shiozaki, Kazuhiro, Akita, Hirotoshi, Wada, Tadashi, ...
Based on the human cDNA sequence predicted to represent the NEU4 sialidase gene in public databases, a cDNA covering the entire coding sequence was isolated from human brain and expressed in...
Urano, Atsushi, Endoh, Masaki, Wada, Tadashi, Morikawa, Yoshihiro, Itoh, Miyuki, Kataoka, Yuki, ...
AF5q31 (also called MCEF) was identified by its involvement in chromosomal translocation with the gene MLL (mixed lineage leukemia), which is associated with infant acute lymphoblastic leukemia....
Aida, Masatoshi, Chen, Yexi, Nakajima, Koichi, Yamaguchi, Yuki, Wada, Tadashi, Handa, Hiroshi
Human 5,6-dichloro-1-β-d-ribofuranosylbenzimidazole sensitivity-inducing factor (DSIF) and negative elongation factor (NELF) negatively regulate transcription elongation by RNA polymerase II...
Tat-SF1 Protein Associates with RAP30 and Human SPT5 Proteins
Kim, Jae B., Yamaguchi, Yuki, Wada, Tadashi, Handa, Hiroshi, Sharp, Phillip A.
The potent transactivator Tat recognizes the transactivation response RNA element (TAR) of human immunodeficiency virus type 1 and stimulates the processivity of elongation of RNA polymerase (Pol) II...
Kakugawa, Yoichiro, Wada, Tadashi, Yamaguchi, Kazunori, Yamanami, Hideaki, Ouchi, Kiyoaki, Sato, Ikuro, ...
Human plasma membrane-associated sialidase (Neu3) is unique in specifically hydrolyzing gangliosides, thought to participate in cell differentiation and transmembrane signaling, thereby playing...
Yamaguchi, Yuki, Inukai, Naoto, Narita, Takashi, Wada, Tadashi, Handa, Hiroshi
Negative elongation factor (NELF) is a human transcription factor complex that cooperates with DRB sensitivity-inducing factor (DSIF)/hSpt4-hSpt5 to repress elongation by RNA polymerase II (RNAPII)....
Narita, Takashi, Yamaguchi, Yuki, Yano, Keiichi, Sugimoto, Seiji, Chanarat, Sittinan, Wada, Tadashi, ...
The multisubunit transcription elongation factor NELF (for negative elongation factor) acts together with DRB (5,6-dichloro-1-β-d-ribofuranosylbenzimidazole) sensitivity-inducing factor (DSIF)/human...
Wada, Tadashi, Takagi, Toshiyuki, Yamaguchi, Yuki, Ferdous, Anwarul, Imai, Takeshi, Hirose, Susumu, ...
We report the identification of a transcription elongation factor from HeLa cell nuclear extracts that causes pausing of RNA polymerase II (Pol II) in conjunction with the transcription inhibitor...
Hartzog, Grant A., Wada, Tadashi, Handa, Hiroshi, Winston, Fred
Previous characterization of the Saccharomyces cerevisiae Spt4, Spt5, and Spt6 proteins suggested that these proteins act as transcription factors that modify chromatin structure. In this work, we...
Human Spt6 Stimulates Transcription Elongation by RNA Polymerase II In Vitro
Endoh, Masaki, Zhu, Wenyan, Hasegawa, Jun, Watanabe, Hajime, Kim, Dong-Ki, Aida, Masatoshi, ...
Recent studies have suggested that Spt6 participates in the regulation of transcription by RNA polymerase II (RNAPII). However, its underlying mechanism remains largely unknown. One possibility,...
Bromodomain Factor 1 (Bdf1) Is Phosphorylated by Protein Kinase CK2
Sawa, Chika, Nedea, Eduard, Krogan, Nevan, Wada, Tadashi, Handa, Hiroshi, Greenblatt, Jack, ...
Bromodomain factor 1 (Bdf1) associates with Saccharomyces cerevisiae TFIID and corresponds to the C-terminal half of higher eukaryotic TAF1. It also associates with the SWR-C complex, which is...
Mandal, Subhrangsu S., Chu, Chun, Wada, Tadashi, Handa, Hiroshi, Shatkin, Aaron J., Reinberg, Danny
Capping of the 5′ ends of nascent RNA polymerase II transcripts is the first pre-mRNA processing event in all eukaryotic cells. Capping enzyme (CE) is recruited to transcription complexes soon...
Kabe, Yasuaki, Yamada, Joe, Uga, Hitoshi, Yamaguchi, Yuki, Wada, Tadashi, Handa, Hiroshi
Osteoclast differentiation factor (ODF)/receptor activator of NF-κB ligand is essential for inducing the differentiation of mature osteoclasts. We find that nuclear factor Y (NF-Y) binds to the...
Evidence for mitochondrial localization of a novel human sialidase (NEU4)
Yamaguchi, Kazunori, Hata, Keiko, Koseki, Koichi, Shiozaki, Kazuhiro, Akita, Hirotoshi, Wada, Tadashi, ...
Based on the human cDNA sequence predicted to represent the NEU4 sialidase gene in public databases, a cDNA covering the entire coding sequence was isolated from human brain and expressed in...
Urano, Atsushi, Endoh, Masaki, Wada, Tadashi, Morikawa, Yoshihiro, Itoh, Miyuki, Kataoka, Yuki, ...
AF5q31 (also called MCEF) was identified by its involvement in chromosomal translocation with the gene MLL (mixed lineage leukemia), which is associated with infant acute lymphoblastic leukemia....
Aida, Masatoshi, Chen, Yexi, Nakajima, Koichi, Yamaguchi, Yuki, Wada, Tadashi, Handa, Hiroshi
Human 5,6-dichloro-1-β-d-ribofuranosylbenzimidazole sensitivity-inducing factor (DSIF) and negative elongation factor (NELF) negatively regulate transcription elongation by RNA polymerase II...
Kubo, Tomohiro, Wada, Tadashi, Yamaguchi, Yuki, Shimizu, Akira, Handa, Hiroshi
Alternative polyadenylation leads to mRNAs with variable 3′ ends. Since a 3′-untranslated region (3′-UTR) often contains cis elements that impact stability or localization of mRNA or...
Adeno-Associated Virus Site-Specific Integration Is Regulated by TRP-185▿
Yamamoto, Noriaki, Suzuki, Masato, Kawano, Masa-aki, Inoue, Takamasa, Takahashi, Ryou-u, Tsukamoto, Hiroko, ...
Adeno-associated virus (AAV) integrates site specifically into the AAVS1 locus on human chromosome 19. Although recruitment of the AAV nonstructural protein Rep78/68 to the Rep binding site (RBS) on...
Zhu, Wenyan, Wada, Tadashi, Okabe, Sachiko, Taneda, Takuya, Yamaguchi, Yuki, Handa, Hiroshi
The transcription elongation factor 5,6-dichloro-1-β-d-ribofuranosylbenzimidazole (DRB) sensitivity-inducing factor (DSIF) regulates RNA polymerase II (RNAPII) processivity by promoting, in concert...
Agawa, Yasuo, Sarhan, Moustafa, Kageyama, Yuji, Akagi, Kazutaka, Takai, Masayoshi, Hashiyama, Kazuya, ...
Regulatory mechanisms controlling the timing of developmental events are crucial for proper development to occur. ftz-f1 is expressed in a temporally regulated manner following pulses of ecdysteroid...
Ando, Kozue, Hirao, Satoshi, Kabe, Yasuaki, Ogura, Yuji, Sato, Iwao, Yamaguchi, Yuki, ...
APE1/Ref-1 is thought to be a multifunctional protein involved in reduction–oxidation (redox) regulation and base excision DNA repair, and is required for early embryonic development in mice....