Takahiro Tougan

Focused Microarray Analysis of Peripheral Mononuclear Blood Cells from Churg-Strauss Syndrome Patients (2008)

Tougan, Takahiro, Onda, Hiroaki, Okuzaki, Daisuke, Kobayashi, Shigeto, Hashimoto, Hiroshi, Nojima, Hiroshi

DNA diagnostics are useful but are hampered by difficult ethical issues. Moreover, it cannot provide enough information on the environmental factors that are important for pathogenesis of certain...

Chum-RNA allows preparation of a high-quality cDNA library from a single-cell quantity of mRNA without PCR amplification (2008)

Tougan, Takahiro, Okuzaki, Daisuke, Nojima, Hiroshi

Linear RNA amplification using T7 RNA polymerase is useful in genome-wide analysis of gene expression using DNA microarrays, but exponential amplification using polymerase chain reaction (PCR) is...

Isolation and Expression Profiling of Genes Upregulated in Bone Marrow-Derived Mononuclear Cells of Rheumatoid Arthritis Patients (2006)

Nakamura, Nobuo, Shimaoka, Yasunori, Tougan, Takahiro, Onda, Hiroaki, Okuzaki, Daisuke, Zhao, Hanjun, ...

We have comprehensively identified the genes whose expressions are augmented in bone marrow-derived mononuclear cells (BMMC) from patients with Rheumatoid Arthritis (RA) as compared with BMMCs from...

Mcp7, a meiosis-specific coiled-coil protein of fission yeast, associates with Meu13 and is required for meiotic recombination (2004)

Saito, Takamune T., Tougan, Takahiro, Kasama, Takashi, Okuzaki, Daisuke, Nojima, Hiroshi

We previously showed that Meu13 of Schizosaccharomyces pombe functions in homologous pairing and recombination at meiosis I. Here we show that a meiosis-specific gene encodes a coiled-coil protein...

Dmc1 of Schizosaccharomyces pombe plays a role in meiotic recombination (2000)

Fukushima, Kentaro, Tanaka, Yoshimi, Nabeshima, Kentaro, Yoneki, Takahiro, Tougan, Takahiro, Tanaka, Seiji, ...

We report here a Schizosaccharomyces pombe gene (dmc1+) that resembles budding yeast DMC1 in the region immediately upstream of the rad24+ gene. We showed by northern and Southern blot...

Replication Factor C3 of Schizosaccharomyces pombe, a Small Subunit of Replication Factor C Complex, Plays a Role in Both Replication and Damage Checkpoints

Shimada, Midori, Okuzaki, Daisuke, Tanaka, Seiji, Tougan, Takahiro, Tamai, Katsuyuki K., Shimoda, Chikashi, ...

We report here the isolation and functional analysis of the rfc3+ gene of Schizosaccharomyces pombe, which encodes the third subunit of replication factor C (RFC3). Because the rfc3+ gene was...

Dmc1 of Schizosaccharomyces pombe plays a role in meiotic recombination

Fukushima, Kentaro, Tanaka, Yoshimi, Nabeshima, Kentaro, Yoneki, Takahiro, Tougan, Takahiro, Tanaka, Seiji, ...

We report here a Schizosaccharomyces pombe gene (dmc1+) that resembles budding yeast DMC1 in the region immediately upstream of the rad24+ gene. We showed by northern and Southern blot analysis that...

The meiotic recombination checkpoint is regulated by checkpoint rad+ genes in fission yeast

Shimada, Midori, Nabeshima, Kentaro, Tougan, Takahiro, Nojima, Hiroshi

During the course of meiotic prophase, intrinsic double-strand breaks (DSBs) must be repaired before the cell can engage in meiotic nuclear division. Here we investigate the mechanism that controls...

Mcp7, a meiosis-specific coiled-coil protein of fission yeast, associates with Meu13 and is required for meiotic recombination

Saito, Takamune T., Tougan, Takahiro, Kasama, Takashi, Okuzaki, Daisuke, Nojima, Hiroshi

We previously showed that Meu13 of Schizosaccharomyces pombe functions in homologous pairing and recombination at meiosis I. Here we show that a meiosis-specific gene encodes a coiled-coil protein...

Spo5/Mug12, a Putative Meiosis-Specific RNA-Binding Protein, Is Essential for Meiotic Progression and Forms Mei2 Dot-Like Nuclear Foci†

Kasama, Takashi, Shigehisa, Akira, Hirata, Aiko, Saito, Takamune T., Tougan, Takahiro, Okuzaki, Daisuke, ...

We report here a functional analysis of spo5+(mug12+) of Schizosaccharomyces pombe, which encodes a putative RNA-binding protein. The disruption of spo5+ caused abnormal sporulation, generating...

Replication Factor C3 of Schizosaccharomyces pombe, a Small Subunit of Replication Factor C Complex, Plays a Role in Both Replication and Damage Checkpoints

Shimada, Midori, Okuzaki, Daisuke, Tanaka, Seiji, Tougan, Takahiro, Tamai, Katsuyuki K., Shimoda, Chikashi, ...

We report here the isolation and functional analysis of the rfc3+ gene of Schizosaccharomyces pombe, which encodes the third subunit of replication factor C (RFC3). Because the rfc3+ gene was...

Dmc1 of Schizosaccharomyces pombe plays a role in meiotic recombination

Fukushima, Kentaro, Tanaka, Yoshimi, Nabeshima, Kentaro, Yoneki, Takahiro, Tougan, Takahiro, Tanaka, Seiji, ...

We report here a Schizosaccharomyces pombe gene (dmc1+) that resembles budding yeast DMC1 in the region immediately upstream of the rad24+ gene. We showed by northern and Southern blot analysis that...

The meiotic recombination checkpoint is regulated by checkpoint rad+ genes in fission yeast

Shimada, Midori, Nabeshima, Kentaro, Tougan, Takahiro, Nojima, Hiroshi

During the course of meiotic prophase, intrinsic double-strand breaks (DSBs) must be repaired before the cell can engage in meiotic nuclear division. Here we investigate the mechanism that controls...

Mcp7, a meiosis-specific coiled-coil protein of fission yeast, associates with Meu13 and is required for meiotic recombination

Saito, Takamune T., Tougan, Takahiro, Kasama, Takashi, Okuzaki, Daisuke, Nojima, Hiroshi

We previously showed that Meu13 of Schizosaccharomyces pombe functions in homologous pairing and recombination at meiosis I. Here we show that a meiosis-specific gene encodes a coiled-coil protein...

Spo5/Mug12, a Putative Meiosis-Specific RNA-Binding Protein, Is Essential for Meiotic Progression and Forms Mei2 Dot-Like Nuclear Foci†

Kasama, Takashi, Shigehisa, Akira, Hirata, Aiko, Saito, Takamune T., Tougan, Takahiro, Okuzaki, Daisuke, ...

We report here a functional analysis of spo5+(mug12+) of Schizosaccharomyces pombe, which encodes a putative RNA-binding protein. The disruption of spo5+ caused abnormal sporulation, generating...

Chum-RNA allows preparation of a high-quality cDNA library from a single-cell quantity of mRNA without PCR amplification

Tougan, Takahiro, Okuzaki, Daisuke, Nojima, Hiroshi

Linear RNA amplification using T7 RNA polymerase is useful in genome-wide analysis of gene expression using DNA microarrays, but exponential amplification using polymerase chain reaction (PCR) is...

Direct Metagenomic Detection of Viral Pathogens in Nasal and Fecal Specimens Using an Unbiased High-Throughput Sequencing Approach

Nakamura, Shota, Yang, Cheng-Song, Sakon, Naomi, Ueda, Mayo, Tougan, Takahiro, Yamashita, Akifumi, ...

With the severe acute respiratory syndrome epidemic of 2003 and renewed attention on avian influenza viral pandemics, new surveillance systems are needed for the earlier detection of emerging...

Focused Microarray Analysis of Peripheral Mononuclear Blood Cells from Churg–Strauss Syndrome Patients

Tougan, Takahiro, Onda, Hiroaki, Okuzaki, Daisuke, Kobayashi, Shigeto, Hashimoto, Hiroshi, Nojima, Hiroshi

DNA diagnostics are useful but are hampered by difficult ethical issues. Moreover, it cannot provide enough information on the environmental factors that are important for pathogenesis of certain...