Human P-selectin glycoprotein ligand-1 is a functional receptor for enterovirus 71 (2008)
Yorihiro Nishimura, Masayuki Shimojima, Yoshio Tano, Tatsuo Miyamura, Takaji Wakita, Hiroyuki Shimizu
Enterovirus 71 (EV71) is a major causative agent of hand, foot, and mouth disease (HFMD), a common febrile disease occurring mainly in young children. Although clinical manifestations of HFMD are...
Someya, Yuichi, Takeda, Naokazu, Wakita, Takaji
The norovirus 3C-like protease is a member of the chymotrypsin-like serine protease superfamily. Previous characterization of its crystal structure has implicated the Glu54–His30–Cys139 triad in...
Zeisel, Mirjam, Koutsoudakis, George, Schnober, Eva, Haberstroh, Anita, Blum, Hubert, Cosset, François-Loïc, ...
Hepatitis C virus (HCV) is a major cause of chronic hepatitis worldwide. Scavenger receptor class B type I (SR-BI) has been shown to bind HCV envelope glycoprotein E2, participate in entry of HCV...
Zeisel, Mirjam, Koutsoudakis, George, Schnober, Eva, Haberstroh, Anita, Blum, Hubert, Cosset, François-Loïc, ...
Hepatitis C virus (HCV) is a major cause of chronic hepatitis worldwide. Scavenger receptor class B type I (SR-BI) has been shown to bind HCV envelope glycoprotein E2, participate in entry of HCV...
Zeisel, Mirjam, Koutsoudakis, George, Schnober, Eva, Haberstroh, Anita, Blum, Hubert, Cosset, François-Loïc, ...
Hepatitis C virus (HCV) is a major cause of chronic hepatitis worldwide. Scavenger receptor class B type I (SR-BI) has been shown to bind HCV envelope glycoprotein E2, participate in entry of HCV...
Replication of a hepatitis C virus replicon clone in mouse cells (2006)
Uprichard, Susan L, Chung, Josan, Chisari, Francis V, Wakita, Takaji
Abstract Background Hepatitis C Virus (HCV) is a significant public health burden and small animal models are needed to study the pathology and immunobiology of the virus. In effort to develop...
The Native Form and Maturation Process of Hepatitis C Virus Core Protein
Yasui, Kohichiroh, Wakita, Takaji, Tsukiyama-Kohara, Kyoko, Funahashi, Shin-Ichi, Ichikawa, Masumi, Kajita, Tadahiro, ...
The maturation and subcellular localization of hepatitis C virus (HCV) core protein were investigated with both a vaccinia virus expression system and CHO cell lines stably transformed with HCV cDNA....
Zhao, Zijiang, Wakita, Takaji, Yasui, Kotaro
We established a simple and effective method for DNA immunization against Japanese encephalitis virus (JEV) infection with plasmids encoding the viral PrM and E proteins and colloidal gold....
Regulation of Hepatitis C Virus Replication by Interferon Regulatory Factor 1
Kanazawa, Nobuhiko, Kurosaki, Masayuki, Sakamoto, Naoya, Enomoto, Nobuyuki, Itsui, Yasuhiro, Yamashiro, Tsuyoshi, ...
Cellular antiviral responses are mediated partly by the expression of interferon-stimulated genes, triggered by viral genomes, their transcripts and replicative intermediates. Persistent replication...
Kato, Takanobu, Date, Tomoko, Miyamoto, Michiko, Zhao, Zijiang, Mizokami, Masashi, Wakita, Takaji
The hepatitis C virus (HCV) genotype 2a subgenomic replicon can replicate in two human non-hepatocyte-derived cell lines, HeLa and 293, with in vitro-transcribed replicon RNA. Sequencing analysis...
Nuclear Localization of Japanese Encephalitis Virus Core Protein Enhances Viral Replication†
Mori, Yoshio, Okabayashi, Tamaki, Yamashita, Tetsuo, Zhao, Zijiang, Wakita, Takaji, Yasui, Kotaro, ...
Japanese encephalitis virus (JEV) core protein was detected in both the nucleoli and cytoplasm of mammalian and insect cell lines infected with JEV or transfected with the expression plasmid of the...
Robust hepatitis C virus infection in vitro
Zhong, Jin, Gastaminza, Pablo, Cheng, Guofeng, Kapadia, Sharookh, Kato, Takanobu, Burton, Dennis R., ...
The absence of a robust cell culture model of hepatitis C virus (HCV) infection has severely limited analysis of the HCV life cycle and the development of effective antivirals and vaccines. Here we...
Cai, Zhaohui, Zhang, Chen, Chang, Kyung-Soo, Jiang, Jieyun, Ahn, Byung-Chul, Wakita, Takaji, ...
Hepatitis C virus (HCV) chronically infects approximately 170 million people worldwide, with an increased risk of developing cirrhosis and hepatocellular carcinoma. The study of HCV replication and...
Kato, Takanobu, Date, Tomoko, Miyamoto, Michiko, Sugiyama, Masaya, Tanaka, Yasuhito, Orito, Etsuro, ...
Although combination therapy with interferon and ribavirin has improved the treatment for chronic hepatitis C virus (HCV) infection, the detailed anti-HCV effect of ribavirin in clinical...
Rouillé, Yves, Helle, François, Delgrange, David, Roingeard, Philippe, Voisset, Cécile, Blanchard, Emmanuelle, ...
Due to the recent development of a cell culture model, hepatitis C virus (HCV) can be efficiently propagated in cell culture. This allowed us to reinvestigate the subcellular localization of HCV...
Yi, MinKyung, Villanueva, Rodrigo A., Thomas, David L., Wakita, Takaji, Lemon, Stanley M.
Infections with hepatitis C virus (HCV) are marked by frequent viral persistence, chronic liver disease, and extraordinary viral genetic diversity. Although much has been learned about HCV since its...
Generation of Infectious Hepatitis C Virus in Immortalized Human Hepatocytes
Kanda, Tatsuo, Basu, Arnab, Steele, Robert, Wakita, Takaji, Ryerse, Jan S., Ray, Ranjit, ...
Progress in understanding hepatitis C virus (HCV) biology has remained a challenge due to the lack of an efficient cell culture system for virus growth. In this study, we examined HCV core...
Diverse Effects of Cyclosporine on Hepatitis C Virus Strain Replication
Ishii, Naoto, Watashi, Koichi, Hishiki, Takayuki, Goto, Kaku, Inoue, Daisuke, Hijikata, Makoto, ...
Recently, a production system for infectious particles of hepatitis C virus (HCV) utilizing the genotype 2a JFH1 strain has been developed. This strain has a high capacity for replication in the...
Hepatitis C Virus Entry Depends on Clathrin-Mediated Endocytosis
Blanchard, Emmanuelle, Belouzard, Sandrine, Goueslain, Lucie, Wakita, Takaji, Dubuisson, Jean, Wychowski, Czeslaw, ...
Due to difficulties in cell culture propagation, the mechanisms of hepatitis C virus (HCV) entry are poorly understood. Here, postbinding cellular mechanisms of HCV entry were studied using both...
Replication of a hepatitis C virus replicon clone in mouse cells
Uprichard, Susan L, Chung, Josan, Chisari, Francis V, Wakita, Takaji
The Native Form and Maturation Process of Hepatitis C Virus Core Protein
Yasui, Kohichiroh, Wakita, Takaji, Tsukiyama-Kohara, Kyoko, Funahashi, Shin-Ichi, Ichikawa, Masumi, Kajita, Tadahiro, ...
The maturation and subcellular localization of hepatitis C virus (HCV) core protein were investigated with both a vaccinia virus expression system and CHO cell lines stably transformed with HCV cDNA....
Zhao, Zijiang, Wakita, Takaji, Yasui, Kotaro
We established a simple and effective method for DNA immunization against Japanese encephalitis virus (JEV) infection with plasmids encoding the viral PrM and E proteins and colloidal gold....
Regulation of Hepatitis C Virus Replication by Interferon Regulatory Factor 1
Kanazawa, Nobuhiko, Kurosaki, Masayuki, Sakamoto, Naoya, Enomoto, Nobuyuki, Itsui, Yasuhiro, Yamashiro, Tsuyoshi, ...
Cellular antiviral responses are mediated partly by the expression of interferon-stimulated genes, triggered by viral genomes, their transcripts and replicative intermediates. Persistent replication...
Kato, Takanobu, Date, Tomoko, Miyamoto, Michiko, Zhao, Zijiang, Mizokami, Masashi, Wakita, Takaji
The hepatitis C virus (HCV) genotype 2a subgenomic replicon can replicate in two human non-hepatocyte-derived cell lines, HeLa and 293, with in vitro-transcribed replicon RNA. Sequencing analysis...
Nuclear Localization of Japanese Encephalitis Virus Core Protein Enhances Viral Replication†
Mori, Yoshio, Okabayashi, Tamaki, Yamashita, Tetsuo, Zhao, Zijiang, Wakita, Takaji, Yasui, Kotaro, ...
Japanese encephalitis virus (JEV) core protein was detected in both the nucleoli and cytoplasm of mammalian and insect cell lines infected with JEV or transfected with the expression plasmid of the...
Robust hepatitis C virus infection in vitro
Zhong, Jin, Gastaminza, Pablo, Cheng, Guofeng, Kapadia, Sharookh, Kato, Takanobu, Burton, Dennis R., ...
The absence of a robust cell culture model of hepatitis C virus (HCV) infection has severely limited analysis of the HCV life cycle and the development of effective antivirals and vaccines. Here we...
Cai, Zhaohui, Zhang, Chen, Chang, Kyung-Soo, Jiang, Jieyun, Ahn, Byung-Chul, Wakita, Takaji, ...
Hepatitis C virus (HCV) chronically infects approximately 170 million people worldwide, with an increased risk of developing cirrhosis and hepatocellular carcinoma. The study of HCV replication and...
Kato, Takanobu, Date, Tomoko, Miyamoto, Michiko, Sugiyama, Masaya, Tanaka, Yasuhito, Orito, Etsuro, ...
Although combination therapy with interferon and ribavirin has improved the treatment for chronic hepatitis C virus (HCV) infection, the detailed anti-HCV effect of ribavirin in clinical...
Rouillé, Yves, Helle, François, Delgrange, David, Roingeard, Philippe, Voisset, Cécile, Blanchard, Emmanuelle, ...
Due to the recent development of a cell culture model, hepatitis C virus (HCV) can be efficiently propagated in cell culture. This allowed us to reinvestigate the subcellular localization of HCV...
Yi, MinKyung, Villanueva, Rodrigo A., Thomas, David L., Wakita, Takaji, Lemon, Stanley M.
Infections with hepatitis C virus (HCV) are marked by frequent viral persistence, chronic liver disease, and extraordinary viral genetic diversity. Although much has been learned about HCV since its...
Generation of Infectious Hepatitis C Virus in Immortalized Human Hepatocytes
Kanda, Tatsuo, Basu, Arnab, Steele, Robert, Wakita, Takaji, Ryerse, Jan S., Ray, Ranjit, ...
Progress in understanding hepatitis C virus (HCV) biology has remained a challenge due to the lack of an efficient cell culture system for virus growth. In this study, we examined HCV core...
Diverse Effects of Cyclosporine on Hepatitis C Virus Strain Replication
Ishii, Naoto, Watashi, Koichi, Hishiki, Takayuki, Goto, Kaku, Inoue, Daisuke, Hijikata, Makoto, ...
Recently, a production system for infectious particles of hepatitis C virus (HCV) utilizing the genotype 2a JFH1 strain has been developed. This strain has a high capacity for replication in the...
Hepatitis C Virus Entry Depends on Clathrin-Mediated Endocytosis
Blanchard, Emmanuelle, Belouzard, Sandrine, Goueslain, Lucie, Wakita, Takaji, Dubuisson, Jean, Wychowski, Czeslaw, ...
Due to difficulties in cell culture propagation, the mechanisms of hepatitis C virus (HCV) entry are poorly understood. Here, postbinding cellular mechanisms of HCV entry were studied using both...
Replication of a hepatitis C virus replicon clone in mouse cells
Uprichard, Susan L, Chung, Josan, Chisari, Francis V, Wakita, Takaji
Wagoner, Jessica, Austin, Michael, Green, Jamison, Imaizumi, Tadaatsu, Casola, Antonella, Brasier, Allan, ...
Hepatitis C virus (HCV) infection induces the α-chemokine interleukin-8 (CXCL-8), which is regulated at the levels of transcription and mRNA stability. In the current study, CXCL-8 regulation by...
E6AP Ubiquitin Ligase Mediates Ubiquitylation and Degradation of Hepatitis C Virus Core Protein▿
Shirakura, Masayuki, Murakami, Kyoko, Ichimura, Tohru, Suzuki, Ryosuke, Shimoji, Tetsu, Fukuda, Kouichirou, ...
Hepatitis C virus (HCV) core protein is a major component of viral nucleocapsid and a multifunctional protein involved in viral pathogenesis and hepatocarcinogenesis. We previously showed that the...
Upregulation of Indoleamine 2,3-Dioxygenase in Hepatitis C Virus Infection▿
Larrea, Esther, Riezu-Boj, José I., Gil-Guerrero, Lucía, Casares, Noelia, Aldabe, Rafael, Sarobe, Pablo, ...
Indoleamine 2,3-dioxygenase (IDO) is induced by proinflammatory cytokines and by CTLA-4-expressing T cells and constitutes an important mediator of peripheral immune tolerance. In chronic hepatitis...
Production of Infectious Hepatitis C Virus of Various Genotypes in Cell Cultures▿
Kato, Takanobu, Matsumura, Takuya, Heller, Theo, Saito, Satoru, Sapp, Ronda K., Murthy, Krishna, ...
A unique hepatitis C virus (HCV) strain JFH-1 has been shown to replicate efficiently in cell culture with production of infectious HCV. We previously developed a DNA expression system containing HCV...
Akazawa, Daisuke, Date, Tomoko, Morikawa, Kenichi, Murayama, Asako, Miyamoto, Michiko, Kaga, Minako, ...
Huh7 cells constitute a permissive cell line for cell culture of hepatitis C virus (HCV) particles. However, our Huh7 line shows limited permissiveness for HCV. Thus, in this study we set out to...
Murayama, Asako, Date, Tomoko, Morikawa, Kenichi, Akazawa, Daisuke, Miyamoto, Michiko, Kaga, Minako, ...
The JFH-1 strain of hepatitis C virus (HCV) is a genotype 2a strain that can replicate autonomously in Huh7 cells. The J6 strain is also a genotype 2a strain, but its full genomic RNA does not...
Monitoring the Antiviral Effect of Alpha Interferon on Individual Cells▿
Kim, Chon Saeng, Jung, Jong Ha, Wakita, Takaji, Yoon, Seung Kew, Jang, Sung Key
An infectious hepatitis C virus (HCV) cDNA clone (JFH1) was generated recently. However, quantitative analysis of HCV infection and observation of infected cells have proved to be difficult because...
Arita, Minetaro, Nagata, Noriyo, Iwata, Naoko, Ami, Yasushi, Suzaki, Yuriko, Mizuta, Katsumi, ...
Enterovirus 71 (EV71) is a causative agent of hand, foot, and mouth disease and is also sometimes associated with serious neurological disorders. In this study, we characterized the antigenicity and...
DDX3 DEAD-Box RNA Helicase Is Required for Hepatitis C Virus RNA Replication▿
Ariumi, Yasuo, Kuroki, Misao, Abe, Ken-ichi, Dansako, Hiromichi, Ikeda, Masanori, Wakita, Takaji, ...
DDX3, a DEAD-box RNA helicase, binds to the hepatitis C virus (HCV) core protein. However, the role(s) of DDX3 in HCV replication is still not understood. Here we demonstrate that the accumulation of...
Arita, Minetaro, Ami, Yasushi, Wakita, Takaji, Shimizu, Hiroyuki
Enterovirus 71 (EV71) is a causative agent of hand, foot, and mouth disease and is also associated with serious neurological disorders. An attenuated EV71 strain [EV71(S1-3′)] has been established...
Critical Role of Virion-Associated Cholesterol and Sphingolipid in Hepatitis C Virus Infection▿
Aizaki, Hideki, Morikawa, Kenichi, Fukasawa, Masayoshi, Hara, Hiromichi, Inoue, Yasushi, Tani, Hideki, ...
In this study, we establish that cholesterol and sphingolipid associated with hepatitis C virus (HCV) particles are important for virion maturation and infectivity. In a recently developed culture...
Masaki, Takahiro, Suzuki, Ryosuke, Murakami, Kyoko, Aizaki, Hideki, Ishii, Koji, Murayama, Asako, ...
Nonstructural protein 5A (NS5A) of the hepatitis C virus (HCV) possesses multiple and diverse functions in RNA replication, interferon resistance, and viral pathogenesis. Recent studies suggest that...
Ariumi, Yasuo, Kuroki, Misao, Dansako, Hiromichi, Abe, Ken-Ichi, Ikeda, Masanori, Wakita, Takaji, ...
Cellular responses to DNA damage are crucial for maintaining genome integrity, virus infection, and preventing the development of cancer. Hepatitis C virus (HCV) infection and the expression of the...
Noroviruses Distinguish between Type 1 and Type 2 Histo-Blood Group Antigens for Binding▿
Shirato, Haruko, Ogawa, Satoko, Ito, Hiromi, Sato, Takashi, Kameyama, Akihiko, Narimatsu, Hisashi, ...
Norovirus (NoV) is a causative agent of acute gastroenteritis. NoV binds to histo-blood group antigens (HBGAs), namely, ABH antigens and Lewis (Le) antigens, in which type 1 and type 2 carbohydrate...
Tajiri-Utagawa, Etsuko, Hara, Masayuki, Takahashi, Kuniaki, Watanabe, Mayumi, Wakita, Takaji
We developed a simple, rapid, high-throughput detection and genotyping method for noroviruses using real-time reverse transcription-PCR (RT-PCR) and high-resolution melting (HRM) analysis to create a...
Kuroki, Misao, Ariumi, Yasuo, Ikeda, Masanori, Dansako, Hiromichi, Wakita, Takaji, Kato, Nobuyuki
Arsenic trioxide (ATO), a therapeutic reagent used for the treatment of acute promyelocytic leukemia, has recently been reported to increase human immunodeficiency virus type 1 infectivity. However,...
Suzuki, Ryosuke, Moriishi, Kohji, Fukuda, Kouichirou, Shirakura, Masayuki, Ishii, Koji, Shoji, Ikuo, ...
We have previously reported on the ubiquitylation and degradation of hepatitis C virus core protein. Here we demonstrate that proteasomal degradation of the core protein is mediated by two distinct...
Hara, Hiromichi, Aizaki, Hideki, Matsuda, Mami, Shinkai-Ouchi, Fumiko, Inoue, Yasushi, Murakami, Kyoko, ...
Persistent infection with hepatitis C virus (HCV) is a major cause of chronic liver diseases. The aim of this study was to identify host cell factor(s) participating in the HCV replication complex...