Maruyama, Yukio, Wakamatsu, Ai, Kawamura, Yoshifumi, Kimura, Kouichi, Yamamoto, Jun-ichi, Nishikawa, Tetsuo, ...
Completion of human genome sequencing has greatly accelerated functional genomic research. Full-length cDNA clones are essential experimental tools for functional analysis of human genes. In one of...
Wakamatsu, Ai, Kimura, Kouichi, Yamamoto, Jun-ichi, Nishikawa, Tetsuo, Nomura, Nobuo, Sugano, Sumio, ...
We analyzed diversity of mRNA produced as a result of alternative splicing in order to evaluate gene function. First, we predicted the number of human genes transcribed into protein-coding mRNAs by...
An Integrated Analysis and Database System for Full-Length cDNA (2007)
Annotation and database system of full-length cDNA sequences was developed. As the components of the system, ORF annotation system, functional annotation system based on database search results,...
Tetsuo Nishikawa, Toshio Ota, Takao Isogai
More than 100 millions of human cDNA fragment sequences have already been published by several EST projects. These ESTs, however, are often incomplete in the 5'-region of full length cDNA...
Tetsuo Nishikawa, Toshio Ota, Takao Isogai
More than 100 millions of human cDNA fragment sequences have already been published by several EST projects. These ESTs, however, are often incomplete in the 5'-region of full length cDNA...
Tetsuo Nishikawa, Jun-ichi Yamamoto, Ai Wakamatsu, Shizuko Ishii, Masashi Nemoto, ...
Whole human genome sequence determination was completed this year. To obtain accurate gene structures and promoter information, full-length cDNA sequence information as well as the genome sequences...
Energy and Industrial Technology Developmental (2007)
Jun-ichi Yamamoto, Naoto Hatano, Kenji Araki, Hiroshi Makita, Kouichi Kimura, Ai Wakamatsu, ...
In the full-length human cDNA sequencing project supported by New
Takeda, Jun-ichi, Suzuki, Yutaka, Nakao, Mitsuteru, Barrero, Roberto A., Koyanagi, Kanako O., Jin, Lihua, ...
We report the first genome-wide identification and characterization of alternative splicing in human gene transcripts based on analysis of the full-length cDNAs. Applying both manual and...
Takeda, Jun-ichi, Suzuki, Yutaka, Nakao, Mitsuteru, Barrero, Roberto A., Koyanagi, Kanako O., Jin, Lihua, ...
We report the first genome-wide identification and characterization of alternative splicing in human gene transcripts based on analysis of the full-length cDNAs. Applying both manual and...
Kimura, Kouichi, Wakamatsu, Ai, Suzuki, Yutaka, Ota, Toshio, Nishikawa, Tetsuo, Yamashita, Riu, ...
By analyzing 1,780,295 5′-end sequences of human full-length cDNAs derived from 164 kinds of oligo-cap cDNA libraries, we identified 269,774 independent positions of transcriptional start sites...
Takeda, Jun-ichi, Suzuki, Yutaka, Nakao, Mitsuteru, Barrero, Roberto A., Koyanagi, Kanako O., Jin, Lihua, ...
We report the first genome-wide identification and characterization of alternative splicing in human gene transcripts based on analysis of the full-length cDNAs. Applying both manual and...
Otsuki, Tetsuji, Ota, Toshio, Nishikawa, Tetsuo, Hayashi, Koji, Suzuki, Yutaka, Yamamoto, Jun-ichi, ...
We have developed an in silico method of selection of human full-length cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries. Fullness rates were increased to about 80% by...
Kimura, Kouichi, Wakamatsu, Ai, Suzuki, Yutaka, Ota, Toshio, Nishikawa, Tetsuo, Yamashita, Riu, ...
By analyzing 1,780,295 5′-end sequences of human full-length cDNAs derived from 164 kinds of oligo-cap cDNA libraries, we identified 269,774 independent positions of transcriptional start sites...
Integrative Annotation of 21,037 Human Genes Validated by Full-Length cDNA Clones (2004)
Tadashi Imanishi, Takeshi Itoh, Yutaka Suzuki, Claire O'Donovan, Satoshi Fukuchi, Kanako O. Koyanagi, ...
An international team has systematically validated and annotated just over 21,000 human genes using full-length cDNA, thereby providing a valuable new resource for the human genetics community.
Integrative Annotation of 21,037 Human Genes Validated by Full-Length cDNA Clones (2004)
Tadashi Imanishi, Takeshi Itoh, Yutaka Suzuki, Claire O'Donovan, Satoshi Fukuchi, Kanako O. Koyanagi, ...
The human genome sequence defines our inherent biological potential; the realization of the biology encoded therein requires knowledge of the function of each gene. Currently, our knowledge in this...
Stochastic Indicator of Regions and Application to (2003)
Protein Coding Region, Kouichi Kimura, Tetsuo Nishikawa, Keiichi Nagai, Sumio Sugano, Nobuo Nomura, ...
Local prediction of regions in a sequence is considered, where the sequence is assumed to be generated by a doubly stochastic Markov model and its transition probabilities are assumed to remain...
The Translated Region Inspector for cDNA Sequences (2003)
Kouichi Kimura, Tetsuo Nisikawa, Keiichi Nagai, Sumio Sugano, Nobuo Nomura, Takao Isogai
Introduction The human genome sequencing project is now being to complete the final stage of building the finished sequences. However, transcriptome sequencing will remain far from completion for...
Nishikawa, Tetsuo, Ota, Toshio, Kawai, Yuri, Ishii, Shizuko, Saito, Kaoru, Yamamoto, Jun-ichi, ...
We compared in detail the characteristics of the sequences of the cDNA clones obtainedb y the oligo-capping method(oligo-capping clones) with that of the sequences in the UniGene database. To compare...
HUNT: launch of a full-length cDNA database from the Helix Research Institute (2001)
Yudate, Henrik T., Suwa, Makiko, Irie, Ryotaro, Matsui, Hiroshi, Nishikawa, Tetsuo, Nakamura, Yoshitaka, ...
The Helix Research Institute (HRI) in Japan is releasing 4356 HUman Novel Transcripts and related information in the newly established HUNT database. The institute is a joint research project...
Suzuki, Yutaka, Tsunoda, Tatsuhiko, Sese, Jun, Taira, Hirotoshi, Mizushima-Sugano, Junko, Hata, Hiroko, ...
Suzuki, Yutaka, Tsunoda, Tatsuhiko, Sese, Jun, Taira, Hirotoshi, Mizushima-Sugano, Junko, Hata, Hiroko, ...
Suzuki, Yutaka, Tsunoda, Tatsuhiko, Sese, Jun, Taira, Hirotoshi, Mizushima-Sugano, Junko, Hata, Hiroko, ...
Nishikawa, Tetsuo, Ota, Toshio, Isogai, Takao
Motivation: In the previous works, we developed ATGpr, a computer program for predicting the fullness of a cDNA, i.e. whether it contains an initiation codon or not. Statistical information of short...
Isogai, Takao, Ono, Hiroki, Ishitani, Yohsuke, Kojo, Hitoshi, Ueda, Yoshio, Kohsaka, Masanobu
D-Amino acid oxidase (DAO) was extracted and purified from cultured mycelia of Fusarium solani M-0718 (FERM P-2688). The enzyme was able to oxidatively deaminate cephalosporin C to...
HUNT: launch of a full-length cDNA database from the Helix Research Institute
Yudate, Henrik T., Suwa, Makiko, Irie, Ryotaro, Matsui, Hiroshi, Nishikawa, Tetsuo, Nakamura, Yoshitaka, ...
The Helix Research Institute (HRI) in Japan is releasing 4356 HUman Novel Transcripts and related information in the newly established HUNT database. The institute is a joint research project...
Identification and Characterization of the Potential Promoter Regions of 1031 Kinds of Human Genes
Suzuki, Yutaka, Tsunoda, Tatsuhiko, Sese, Jun, Taira, Hirotoshi, Mizushima-Sugano, Junko, Hata, Hiroko, ...
To understand the mechanism of transcriptional regulation, it is essential to identify and characterize the promoter, which is located proximal to the mRNA start site. To identify the promoters from...
Integrative Annotation of 21,037 Human Genes Validated by Full-Length cDNA Clones
Imanishi, Tadashi, Itoh, Takeshi, Suzuki, Yutaka, O'Donovan, Claire, Fukuchi, Satoshi, Koyanagi, Kanako O, ...
The human genome sequence defines our inherent biological potential; the realization of the biology encoded therein requires knowledge of the function of each gene. Currently, our knowledge in this...
Diverse transcriptional initiation revealed by fine, large-scale mapping of mRNA start sites
Suzuki, Yutaka, Taira, Hirotoshi, Tsunoda, Tatsuhiko, Mizushima-Sugano, Junko, Sese, Jun, Hata, Hiroko, ...
Determination of the mRNA start site is the first step in identifying the promoter region, which is of key importance for transcriptional regulation of gene expression. The ‘oligo-capping’ method...
Kimura, Kouichi, Wakamatsu, Ai, Suzuki, Yutaka, Ota, Toshio, Nishikawa, Tetsuo, Yamashita, Riu, ...
By analyzing 1,780,295 5′-end sequences of human full-length cDNAs derived from 164 kinds of oligo-cap cDNA libraries, we identified 269,774 independent positions of transcriptional start sites...
Takeda, Jun-ichi, Suzuki, Yutaka, Nakao, Mitsuteru, Barrero, Roberto A., Koyanagi, Kanako O., Jin, Lihua, ...
We report the first genome-wide identification and characterization of alternative splicing in human gene transcripts based on analysis of the full-length cDNAs. Applying both manual and...
HUNT: launch of a full-length cDNA database from the Helix Research Institute
Yudate, Henrik T., Suwa, Makiko, Irie, Ryotaro, Matsui, Hiroshi, Nishikawa, Tetsuo, Nakamura, Yoshitaka, ...
The Helix Research Institute (HRI) in Japan is releasing 4356 HUman Novel Transcripts and related information in the newly established HUNT database. The institute is a joint research project...
Identification and Characterization of the Potential Promoter Regions of 1031 Kinds of Human Genes
Suzuki, Yutaka, Tsunoda, Tatsuhiko, Sese, Jun, Taira, Hirotoshi, Mizushima-Sugano, Junko, Hata, Hiroko, ...
To understand the mechanism of transcriptional regulation, it is essential to identify and characterize the promoter, which is located proximal to the mRNA start site. To identify the promoters from...
Integrative Annotation of 21,037 Human Genes Validated by Full-Length cDNA Clones
Imanishi, Tadashi, Itoh, Takeshi, Suzuki, Yutaka, O'Donovan, Claire, Fukuchi, Satoshi, Koyanagi, Kanako O, ...
The human genome sequence defines our inherent biological potential; the realization of the biology encoded therein requires knowledge of the function of each gene. Currently, our knowledge in this...
Diverse transcriptional initiation revealed by fine, large-scale mapping of mRNA start sites
Suzuki, Yutaka, Taira, Hirotoshi, Tsunoda, Tatsuhiko, Mizushima-Sugano, Junko, Sese, Jun, Hata, Hiroko, ...
Determination of the mRNA start site is the first step in identifying the promoter region, which is of key importance for transcriptional regulation of gene expression. The ‘oligo-capping’ method...
Kimura, Kouichi, Wakamatsu, Ai, Suzuki, Yutaka, Ota, Toshio, Nishikawa, Tetsuo, Yamashita, Riu, ...
By analyzing 1,780,295 5′-end sequences of human full-length cDNAs derived from 164 kinds of oligo-cap cDNA libraries, we identified 269,774 independent positions of transcriptional start sites...
Takeda, Jun-ichi, Suzuki, Yutaka, Nakao, Mitsuteru, Barrero, Roberto A., Koyanagi, Kanako O., Jin, Lihua, ...
We report the first genome-wide identification and characterization of alternative splicing in human gene transcripts based on analysis of the full-length cDNAs. Applying both manual and...
Maruyama, Yukio, Wakamatsu, Ai, Kawamura, Yoshifumi, Kimura, Kouichi, Yamamoto, Jun-ichi, Nishikawa, Tetsuo, ...
Completion of human genome sequencing has greatly accelerated functional genomic research. Full-length cDNA clones are essential experimental tools for functional analysis of human genes. In one of...
Wakamatsu, Ai, Kimura, Kouichi, Yamamoto, Jun-ichi, Nishikawa, Tetsuo, Nomura, Nobuo, Sugano, Sumio, ...
We analyzed diversity of mRNA produced as a result of alternative splicing in order to evaluate gene function. First, we predicted the number of human genes transcribed into protein-coding mRNAs by...