Scientific Commons: Takao Tsuji

Optimal Bidding Strategies for Generation Companies in a Day-Ahead Electricity Market with Risk Management Taken into Account (2009)

Azmi Saleh, Takao Tsuji, Tsutomu Oyama

Problem statement: In a competitive electricity market with limited number of producers, Generation Companies (Gencos) is facing an oligopoly market rather than a perfect competition. Under oligopoly...

Optimal Bidding Strategies for a Small Generation Company in a Day-Ahead Electricity Market with Bilateral Contracts Taken into Account (2009)

Azmi Saleh, Takao Tsuji, Tsutomu Oyama

Problem Statement: Deregulation power systems have been force to change their structures, from vertically integrated to open market systems. Each generation company (Genco) is required to compete...

Nucleotide sequence analysis of the enterotoxigenic Escherichia coli Ent plasmid (2009)

Ochi, Sadayuki, Shimizu, Tohru, Ohtani, Kaori, Ichinose, Yoshio, Arimitsu, Hideyuki, Tsukamoto, Kentaro, ...

We report here the complete nucleotide sequence of pEntH10407 (65 147 bp), an enterotoxigenic Escherichia coli enterotoxin plasmid (Ent plasmid), which is self-transmissible at low frequency. Within...

An immunofluorescent study on anti-synovial tissue antibody in the body fluid from patients with rheumatoid arthritis (2007)

Ogawa, Katsuo, Tsuji, Takao, Namba, Masayoshi, Hayama, Kazuo, Okamoto, Tsukasa, Miwa, Yasuhiko

There is as yet no plausible and convincing explanation for the etiology of rheumatoid arthritis. The authors investigated anti-synovial tissue antibody in the body fluid of rheumatoid arthritis by...

Tissue localization of C1q in HBs antigen positive liver disease patients by direct immunofluorescent technique (2007)

Tsuji, Takao, Naito, Kunihiko, Araki, Kiyonori, Onoue, Kimiaki, Nozaki, Hajime, Nagashima, Hideo

Tissue localization of a subcomponent of the first component of complement (CLq) was examined in one postmortem case of HBs antigen (HBs Ag) positive hepatocellular carcinoma and in six cases of...

Detection of HBs antigen in routine paraffin embedded liver tissue by enzyme-labelled antibody technique (2007)

Tsuji, Takao, Naito, Kunihiko, Tokuyama, Katsuyuki, Okada, Takeshi, Kosaka, Kiyowo

HB surface antigen (HBs Ag) was detected using the enzyme-labelled antibody technique on routinely processed liver biopsy material fixed in Bouin's fixative and embedded in paraffin. Of 85 examined...

Detection of liver HBc antigen and its antibody in sera from viral hepatitis by the immunofluorescent complement technique (2007)

Tsuji, Takao, Tokuyama, Katsuyuki, Okada, Takeshi, Takata, Shinji, Naito, Kunihiko, Kosaka, Kiyowo, ...

Hepatitis B core antigen (HBc Ag) and hepatitis B surface antigen (HBs Ag) were detected in the liver tissue of a patient with chronic aggressive hepatitis by the immunofluorescent complement...

Tissue immune complexes demonstrated in the liver of patients with chronic aggressive hepatitis using FITC-labelled human Clq. (2007)

Tsuji, Takao, Araki, Kiyonori, Inoue, Junichi, Onoue, Kimiaki, Tsuchiya, Masao, Shinohara, Toru, ...

Immune complexes in liver specimens from 10 patients with chronic liver diseases [2 with chronic persistent hepatitis (CPH), 3 with chronic aggressive hepatitis (CAH) of moderate activity, 3 with CAH...

Detection and characterization of circulating immune complexes during acute exacerbation of chronic viral hepatitis. (2007)

Araki, Kiyonori, Tsuji, Takao, Onoue, Kimiaki, Tuchiya, Masao, Shinohara, Toru, Inoue, Junichi, ...

For the detection and characterization of circulating immune complexes (CIC) in various liver diseases, a Clq binding test was used. Though the CIC level was almost normal in HB surface antigen...

Existence of serum HBe antigen and expression of liver HB surface and core antigens in hepatitis type B patients. (2007)

Tsuji, Takao, Naito, Kunihiko, Inoue, Junichi, Tsuchiya, Masao, Araki, Kiyonori, Shinohara, Toru, ...

A study of 52 liver biopsies (47 hepatitis type B and 5 asymptomatic carriers) was performed to clarify the roles of HBe antigen (HBeAg), HB surface antigen (HBsAg) and HB core antigen (HBcAg). In...

Detection and characterization of antibody to liver cell membrane in sera from patients with chronic active liver diseases. (2007)

Tsuji, Takao, Araki, Kiyonori, Naito, Kunihiko, Inoue, Junichi, Nagashima, Hideo

Sera from 84 patients with chronic liver disease [CLD] (74 chronic active) and from 53 blood donors were tested immunochemically for anti-liver cell membrane antibody (LMAb). LMAb to rat liver tested...

Detection of tissue T-cell in patients with chronic active hepatitis using fragmented sheep red blood cell (SRBC) membrane. (2007)

Tsuji, Takao, Inoue, Junichi, Shinohara, Toru, Tsuchiya, Masao, Onoue, Kimiaki, Araki, Kiyonori, ...

Fragmented sheep red blood cell (SRBC) membrane was used for detection of T-cells in liver biopsy specimens from patients with chronic active hepatitis. SRBC was separated with Lymphoprep, sonicated,...

Surgical treatment for Crohn's disease. (2007)

Perdomo, Jose Antonio, Iwagaki, Hiromi, Hizuta, Akio, Mizuno, Motowo, Nakagawa, Hitoshi, Tanaka, Noriaki, ...

The medical records of 16 consecutive patients with Crohn's disease surgically treated in our department from 1978 to 1993 were retrospectively reviewed. The indication for surgery was obstructive...

Immunohistochemical studies of PIVKA-II in hepatocellular carcinoma by indirect immunofluorescence (2007)

Kuwahara, Naoaki, Higashi, Toshihiro, Nouso, Kazuhiro, Ito, Toshio, Tsuji, Takao

Tissue PIVKA-II was examined in 32 hepatocellular carcinomas and 2 metastatic liver tumors using indirect immunofluorescence, and the results were compared with the size, histological grading and...

Immunoelectron Microscopic Localization of MHC Class 1 and 2 Antigens on Bile Duct Epithelial Cells in Patients with Primary Biliary Cirrhosis (2007)

Nishimoto, Hiroshi, Yamada, Gotaro, Mizuno, Motowo, Tsuji, Takao

We studied the distribution of class 1 and class 2 major histocompatibility complex (MHC) antigens on bile duct epithelial cells in liver from patients with primary biliary cirrhosis (PBC) by an...

Intrafamilial clustering of genotypes of hepatitis C virus RNA. (2007)

Takahashi, Michiko, Yamada, Gotaro, Doi, Toshihiko, Takatani, Masahiro, Kishi, Fumitoshi, Miyamoto, Rieko, ...

Hepatitis C virus (HCV)-RNA in the blood was measured by polymerase chain reaction (PCR) in 37 subjects from eight families in which 2 or more persons tested seropositive for antibodies against...

Distribution of complement regulatory proteins, decay-accelerating factor, CD59/homologous restriction factor 20 and membrane cofactor protein in human colorectal adenoma and cancer. (2007)

Inoue, Hiroshi, Mizuno, Motowo, Uesu, Tokurou, Ueki, Toru, Tsuji, Takao

To clarify the events related to complement-mediated immune responses in human colorectal cancers, we immunohistochemically examined the distribution of decay-accelerating factor (DAF),...

Immunohistochemical analysis of epithelial cell proliferation in normal-appearing rectal mucosa of patients with colorectal adenoma and cancer using an in vitro labeling method with bromodeoxyuridine. (2007)

Ikeda, Nobumasa, Mizuno, Motowo, Okada, Hiroyuki, Tomoda, Jun, Tsuji, Takao

To identify diffuse mucosal changes which may precede the development of colorectal cancer and a possible indicator for detecting high-risk populations, we immunohistochemically studied cell-cycle...

Endoscopical Segmental Piecemeal Tumorectomy for Nodular Elevation of Colorectal Tumor: Applicability and Patient's Quality of Life (2007)

Ariki, Norifumi, Iwagaki, Hiromi, Yoshino, Tadashi, Nonaka, Yasuyuki, Fujiki, Shigeatsu, Perdomo, Jose Antonio, ...

Endoscopical segmental piecemeal tumorectomy (ESPT) for nodular elevation of colorectal tumor is advantageous in terms of minimizing both surgical invasion and postoperative burden to the patients....

Evaluation of serum uric acid to creatinine ratio in fulminant hepatitis. (2007)

Omori, Nobuhiko, Ukida, Minoru, Mikami, Masayuki, Morimoto, Youichi, Tsuji, Takao

Of the eight patients with fulminant hepatitis placed under total parenteral nutrition with an amino acid solution rich in branched chain amino acids and treated by plasma exchange, four survived and...

Quantitation of Hepatitis C Virus RNA in Liver Tissue as a Predictive Marker of the Response to Interferon Therpay in Chronic Hepatitis C (2007)

Hasui, Toshimi, Shimomura, Hiroyuki, Tsuji, Hideyuki, Wato, Masaki, Tsuji, Takao

Recently, factors predicting the response to interferon (IFN) therapy against hepatitis C virus (HCV) have received much attention. To evaluate the usefulness of the quantitation of intrahepatic HCV...

Specificities and Clinical Significance of Anti-Cytoskeleton Antibodies in Anti-Smooth Muscle Antibody-Positive Patients with Chronic Liver Disease C (2007)

Takaki, Akinobu, Sakaguchi, Kohsaku, Ogawa, Shin, Kawamoto, Hirofumi, Tsuji, Takao

We investigated the specificities and characteristics of anti-cytoskeleton antibodies in 13 anti-smooth muscle antibody (ASMA)-positive patients with chronic liver disease C (CLD-C), and compared...

Administration of a Branched-Chain Amino Acid Preparation during Hepatic Failure: A Study Emphasizing Ammonia Metabolism (2007)

Nishikawa, Yoshiyuki, Ukida, Minoru, Matsuo, Ryuichi, Morimoto, Youichi, Omori, Nobuhiko, Mikami, Masayuki, ...

We administered a branched-chain amino acid (BCAA) infusion to 16 patients with hepatic failure and two healthy subjects, and then evaluated its effects on ammonia metabolism and amino acid metabolic...

Gene expression of liver-specific proteins in hepatocyte spheroids in primary culture. (2007)

Tamura, Tomoyuki, Koide, Norio, Hada, Hajime, Shiraha, Hidenori, Tsuji, Takao

Adult rat hepatocytes assemble to form multicellular spheroids under non-adherent environments such as immobilized chondroitin sulfate-proteoglycan in primary culture. Previously, we demonstrated...

Increased urinary excretion of non-albumin antigen detected with YO-2, a novel monoclonal antibody, in diabetic patients. (2007)

Yonei, Taiji, Watarai, Shinobu, Okada, Yoshio, Yasuda, Tatsuji, Tsuji, Takao

Monoclonal antibodies were raised against urine proteins from diabetic patients. An antibody, YO-2, stained three protein bands with apparent molecular weights of 66, 49, and 36 kDa. These bands were...

Expression of ICAM-I on M cells covering isolated lymphoid follicles of the human colon. (2007)

Ueki, Toru, Mizuno, Motowo, Uesu, Tokurou, Kiso, Takahiko, Tsuji, Takao

To clarify the immunological function of 'M' (microfold or membranous) cells in the large intestine, we examined the expression of intercellular adhesion molecule-1 (ICAM-1) and HLA-class II antigens...

Virological and serological characterization of asymptomatic blood donors positive for anti-hepatitis C virus antibody. (2007)

Tsuji, Hideyuki, Shimomura, Hiroyuki, Wato, Masaki, Kondo, Junichi, Tsuji, Takao

To study the virological and serological characteristics of asymptomatic hepatitis C virus (HCV) carriers, 165 blood donors positive for antibody against HCV proteins by the second generation assay,...

A 15N GC/MS Study of in Vivo Glutamine Synthesis in Liver Failure Rats (2007)

Morishita, Hirohumi, Ukida, Minoru, Morimoto, Youichi, Mikami, Masayuki, Tsuji, Takao

To clarify the nature of nitrogen metabolism between branched chain amino acid (BCAA) and glutamine (GIn) in liver failure, we measured arterial plasma concentrations of GIn and 15N uptake to amino-N...

Geranylgeranylacetone and cetraxate hydrochloride increase UDP-galactosyltransferase activity in rat gastric mucosa (2007)

Mikami, Yuichirou, Mizuno, Motowo, Maga, Toshirou, Kihara, Yasuhiro, Yoshinaga, Fumiya, Tanaka, Shouichi, ...

UDP-galactosyltransferase (UDP-Gal-T) is a key enzyme in the synthesis of mucus glycoprotein which plays an important role in gastric mucosal defensive mechanisms. Analysis of gastric UDP-Gal-T...

Increased serum levels of the carrier molecules of the carbohydrate antigen sialyl Lewis X in liver diseases (2007)

Sakai, Nobuyuki, Okada, Yoshio, Tsuji, Takao

The serum levels of the carbohydrate antigen sialyl Lewis X (SLEX) increase in liver diseases (Sunayama T, Okada Y, Tsuji T., J Hepatol 1994; 19: 451-458). However, it is not known whether the...

Cathepsin B in the Growth of Colorectal Cancer: Increased Activity of Cathepsin B in Human Colorectal Cancer (2007)

Satoh, Yasumasa, Higashi, Toshihiro, Nouso, Kazuhiro, Shiota, Tetsuya, Kinugasa, Nobuyuki, Yoshida, Keigo, ...

Cathepsin B, a thiol protease, is involved in cancer metastasis. To clarify the role of cathepsin B in tumor progression in human colorectal cancer, the relationship between its activity,...

Cathepsin B in the Growth of Colorectal Cancer: Suppressive Effect of Leupeptin on the Growth of DMH-Induced Rat Colon Neoplasm (2007)

Satoh, Yasumasa, Higashi, Toshihiro, Nouso, Kazuhiro, Shiota, Tetsuya, Kinugasa, Nobuyuki, Yoshida, Keigo, ...

Cathepsin B, a thiol protease, has been reported to be involved in cancer progression and metastasis. The suppressive effects of two kinds of protease inhibitors, leupeptin and dietary camostate...

Usefulness of indocyanine green injection during ultrasound-guided liver biopsy for the diagnosis of small hepatocellular carcinoma. (2007)

Kimura, Yoshihiro, Higashi, Toshihiro, Kuwahara, Naoaki, Nouso, Kazuhiro, Ohguchi, Shouhei, Hino, Naoki, ...

To diagnose hepatocellular carcinoma (HCC) functionally and immediately, we examined the usefulness of indocyanine green (ICG) injection during ultrasound-guided liver biopsy. Liver specimens were...

Primary Sclerosing Cholangitis in Japanese Patients: Association with Inflammatory Bowel Disease (2007)

Okada, Hiroyuki, Mizuno, Motowo, Yamamoto, Kazuhide, Tsuji, Takao

To characterize primary sclerosing cholangitis (PSC) in Japanese patients and its association with inflammatory bowel disease (IBD), 155 reported cases of PSC, including 6 cases of our own, were...

Comarison of Various Methods of Assaying the Cytotoxic Effects of Ethanol on Human Hepatoblastoma Cells (HUH-6 Line) (2007)

Kosaka, Tsunenori, Fukaya, Ken-ichi, Tsuboi, So, Pu, Hong, Ohno, Tadao, Tsuji, Takao, ...

The sensitivity of five kinds of cytotoxicity assays using ethanol on human hepatoblastoma cells (HUH-6 line), which were cultured as monolayers or spheroids, was compared. Ethanol was chosen as a...

Relationship between Response to Interferon Therapy and Detection of Hepatitis C Virus RNA by Differential Flotation Centrifugation (2007)

Wato, Masaki, Shimomura, Hiroyuki, Fujio, Kozo, Tsuji, Hideyuki, Kondo, Junichi, Fujioka, Shin-ichi, ...

We purified an apurinic/apyrimidinic (AP) endonuclease from mouse ascites sarcoma (SR-C3H/He) cells. The enzyme showed nicking activity on acid-depurinated DNA but not on untreated, intact DNA. It...

Purification and characterization of a 39kDa apurinic/apyrimidinic endonuclease from mouse ascites sarcoma cells. (2007)

Wakabayashi, Hajime, Tsuji, Takao, Seki, Shuji

We purified an apurinic/apyrimidinic (AP) endonuclease from mouse ascites sarcoma (SR-C3H/He) cells. The enzyme showed nicking activity on acid-depurinated DNA but not on untreated, intact DNA. It...

Spheroid cultures of human hepatoblastoma cells (HuH-6 line) and their application for cytotoxicity assay of alcohols. (2007)

Kosaka, Tsunenori, Tsuboi, So, Fukaya, Ken-ichi, Pu, Hong, Ohno, Tadao, Tsuji, Takao, ...

Spheroid cultures of human hepatoblastoma cells (HuH-6 line) were established by rotating 3 x 10(6) cells/3 ml culture medium in 25-ml Erlenmeyer flasks on a gyratory shaker. The size of the...

Spheroid Cultures of Human Hepatoblastoma Cells (HuH-6 Line) and Their Application for Cytotoxicity Assay of Alcohols (2007)

Kosaka, Tsunenori, Tsuboi, So, Fukaya, Ken-ichi, Pu, Hong, Ohno, Tadao, Tsuji, Takao, ...

Spheroid cultures of human hepatoblastoma cells (HuH-6 line) were established by rotating 3 x 10(6) cells/3 ml culture medium in 25-ml Erlenmeyer flasks on a gyratory shaker. The size of the...

Recombinant mouse cytochromes P1-450 and P3-450: enzymatic characterization of the hemoprotein expressed in human cells infected with recombinant vaccinia virus. (2007)

Nouso, Kazuhiro, Battula, Narayama, Thorgeirsson, Snorri S, Higashi, Toshihiro, Tsuji, Takao

We expressed mouse cytochrome P1-450 and P3-450 using recombinant vaccinia virus gene expression system in HeLa cells that were devoid of significant basal levels of P-450. HeLa cells were infected...

Survival simulation of hepatocellular carcinoma derived from follow-up studies of 450 patients. (2007)

Kakio, Takeshi, Ito, Toshio, Sue, Kunihiko, Tanimizu, Masahito, Tsuji, Takao

A simulation model to predict the survival probability of individual patients with hepatocellular carcinoma (HCC) after therapy was derived from the results of various therapies and follow-up studies...

Detection of negative strand RNA of hepatitis C virus in infected liver and serum. (2007)

Nakagawa, Hiroshi, Shimomura, Hiroyuki, Hasui, Toshimi, Tsuji, Hideyuki, Tsuji, Takao

The negative strand RNA of hepatitis C virus, supposed to be a replicative intermediate of the virus appears to indicate viral replication. In this study, we detected the negative strand RNA by using...

Autoimmune responses as assessed by hypergammaglobulinemia and the presence of autoantibodies in patients with chronic hepatitis C. (2007)

Kawamoto, Hirofumi, Sakaguchi, Kohsaku, Takaki, Akinobu, Ogawa, Shin, Tsuji, Takao

We investigated autoimmunity, as assessed by hypergammaglobulinemia and the presence of autoantibodies including anti-nuclear antibodies (ANA) and anti-liver membrane antibodies (LMA), in 149...

Loss of ICG uptake in the process of rat hepatocarcinogenesis correlates to the disappearance of glutathione-S-transferase alpha subunit. (2007)

Ling, Liu, Higashi, Toshihiro, Tsuchida, Shigeki, Sato, Kiyomi, Tsuji, Takao

Reduced indocyanine green (ICG) uptake is one of the functional changes of human hepatocellular carcinoma (HCC). To clarify the mechanisms of loss of ICG uptake, and determine which subunit of...

Disappearance of pulmonary metastases by OK-432 treatment in a case of hepatocellular carcinoma. (2007)

Hino, Naoki, Higashi, Toshihiro, Ueki, Tooru, Nakatsukasa, Harushige, Ooguchi, Souhei, Ashida, Kouzou, ...

We report here a case of hepatocellular carcinoma (HCC) with multiple lung metastases, which were disappeared by treatment of OK-432. The patient was a 65-year-old man and was diagnosed in 1986 with...

Detection by western blotting of an antibody to the hepatitis C virus E1 envelope protein in sera of patients with chronic liver disease. (2007)

Hada, Hajime, Koide, Norio, Hanafusa, Tadashi, Sakaguchi, Kosaku, Shinji, Toshiyuki, Sasaki, Shunsuke, ...

We detected an antibody to HCV envelope protein (E1) in sera of patients with HCV-related chronic liver diseases (20 patients with chronic hepatitis and 5 patients with liver cirrhosis) by Western...

Localization of hepatitis C virus RNA in human liver biopsies by in situ hybridization using thymine-thymine dimerized oligo DNA probes: improved method. (2007)

Endo, Hisashi, Yamada, Gotaro, Nakane, Paul K, Tsuji, Takao

To establish the most proper method of in situ hybridization in detection of HCV-RNA in the liver, various detailed procedures were examined using frozen as well as paraffin-embedded sections of...

The role of Kupffer cells in complement activation in D-Galactosamine/lipopolysaccharide-induced hepatic injury of rats. (2007)

Matsuo, Ryuichi, Ukida, Minoru, Nishikawa, Yoshiyuki, Omori, Nobuhiko, Tsuji, Takao

To investigate the role of Kupffer cells in complement activation, we used a rat model of acute hepatic injury induced by D-Galactosamine (GalN) and lipopolysaccharide (LPS). In in vivo study,...

NG-nitro-L-arginine attenuates flow debt repayment in the reactive hyperemic response of the open-chest dog coronary artery: contribution of endothelium-derived relaxing factor. (2007)

Marutani, Morio, Kusachi, Shouzo, Kajikawa, Yutaka, Yamasaki, Satoshi, Tsuji, Takao

To test the hypothesis that the endothelium-derived relaxing factor (EDRF) contributes to coronary vasodilation induced by myocardial ischemia, we examined the effect of NG-nitro-L-arginine (a potent...

Hepatitis C virus antibody titration in patients with chronic hepatitis C, before and after interferon treatment. (2007)

Takahashi, Michiko, Yamada, Gotaro, Miyamoto, Rieko, Doi, Toshihiko, Endo, Hisashi, Nishimoto, Hiroshi, ...

We measured hepatitis C virus antibody titers in 13 patients with chronic hepatitis C to determine whether titration of hepatitis C virus antibody was useful or not, to predict and evaluate the...

Anti-C100-3 antibody status, viral genomic sequences, and clinical features in chronic hepatitic patients with hepatitis C virus RNA in sera. (2007)

Morii, Kazuhiko, Shimomura, Hiroyuki, Nakagawa, Hiroshi, Hasui, Toshimi, Tsuji, Takao

Since detection of hepatitis C virus RNA by the polymerase chain reaction (PCR) showed that there existed anti-C100-3 (anti-HCV) antibody negative patients infected with HCV, we attempted to find out...

Combination of epidermal growth factor and insulin is required for multicellular spheroid formation of rat hepatocytes in primary culture. (2007)

Kawaguchi, Mitsuhiko, Koide, Norio, Sakaguchi, Kohsaku, Shinji, Toshiyuki, Tsuji, Takao

We showed that the combination of epidermal growth factor (EGF) and insulin is an essential supplement to Williams' #E medium for the formation of floating multicellular spheroids in primary culture...

Rat liver nodules induced by 2-acetylaminofluorene lose an ability to take up indocyanine green in the process of hepatocarcinogenesis. (2007)

Sato, Atsuhiko, Higashi, Toshihiro, Ling, Liu, Shiota, Tetsuya, Tsuji, Takao

Indocyanine green (ICG) was injected into rat liver nodules induced by 2-acetylaminofluorene (2-AAF) via portal vein. The relationship between ICG staining and cell atypism of liver nodules was...

Sequence variations in the envelope protein of the hepatitis C virus: comparison with partial cDNA sequence of a new variant virus obtained by the polymerase chain reaction. (2007)

Hada, Hajime, Koide, Norio, Takabatake, Hiroyuki, Hanafusa, Tadashi, Tsuji, Takao

It has been reported that the envelope region located at the 3' portion of the structural protein coding region is one of the most variable regions at both nucleotide and amino acid sequence levels...

Analysis of galactosyltransferase activity in rat gastric mucosa using crude mucosal homogenate. (2007)

Hongo, Toshiki, Tomoda, Jun, Mizuno, Motowo, Maga, Toshirou, Tsuji, Takao

Mucus glycoprotein is one of the major components of gastric mucus which plays an important role in mucosal defensive mechanisms as a mucus-bicarbonate barrier. Analysis of the mucus glycoprotein...

Increase in cell surface wheat germ agglutinin binding in a rat hepatoma cell line dRLa 74 treated with concanavalin A. (2007)

Shimoe, Tosinari, Okada, Yoshio, Tsuji, Takao

Wheat germ agglutinin binding to a rat hepatoma cell line dRLa 74 treated with concanavalin A was studied. It increased depending on the concanavalin A concentration in the culture medium. The cells...

Immunohistochemical studies on the class and the subclass of the anticolon antibody and the distribution of the antigen recognized by the anticolon antibody in patients with ulcerative colitis. (2007)

Kanzaki, Kohji, Mizuno, Motowo, Tsuji, Takao

The immunologic mechanisms mediated by anticolon antibodies have been suggested for the injury of colonic mucosa in ulcerative colitis (UC). For the understanding of pathogenetic relevance of the...

Genetic variation of putative core gene in hepatitis C virus. (2007)

Fujio, Kozo, Shimomura, Hiroyuki, Tsuji, Takao

Genetic variation of hepatitis C virus was assessed. We prepared RNA fractions from 21 patients' sera which were positive for hepatitis C virus RNA, synthesized their cDNAs, and amplified fragments,...

Immunohistochemical characterization of the lymphocyte and the immunoglobulin-containing cell in the epithelium and the lamina propria of normal human intestines. (2007)

Matsueda, Kazuhiro, Yamada, Gotaro, Tsuji, Takao

In order to clarify difference of the mucosal immunity in various sites of normal large and small intestines, we studied the population of lymphocyte subsets and immunoglobulin (Ig)-containing cells...

Association between high grade ventricular arrhythmia and extent of left ventricular hypertrophy in hypertrophic cardiomyopathy. (2007)

Cheng, Xiao-shu, Kusachi, Shozo, Urabe, Norio, Nogami, Kunio, Takemoto, Masao, Morishita, Naoya, ...

The association between the extent of left ventricular (LV) hypertrophy and severity of ventricular or atrial arrhythmias are examined. Two-dimensional echocardiography and 24-h Holter...

Hepatitis B virus markers in patients with schistosomiasis, liver cirrhosis and hepatocellular carcinoma in Khartoum, Sudan. (2007)

Itoshima, Tatsuya, Fedail, Sulieman S., Suliman, Ikhlas, Ali, Abbas K., Higashi, Toshihiro, Tsuji, Takao

Markers of hepatitis A and B virus were tested in 88 adult Sudanese subjects in Khartoum, Sudan. The subjects consisted of 25 control hospitalized patients, 21 volunteer blood donors, 23 patients...

Factors influencing acute high-grade restenosis in emergency percutaneous transluminal coronary angioplasty for acute myocardial infarction. (2007)

Kusachi, Shozo, Iwasaki, Kohichirou, Nishiyama, Osamu, Ueda, Minoru, Kita, Toshimasa, Hata, Takato, ...

We studied the factors which may induce acute high grade restenosis in emergency percutaneous transluminal coronary angioplasty (PTCA). PTCA was attempted in 50 patients with acute myocardial...

Lipid malnutrition of patients with liver cirrhosis: effect of low intake of dietary lipid on plasma fatty acid composition. (2007)

Okita, Misako, Watanabe, Akihara, Tsuji, Takao

The plasma fatty acid composition of cirrhotic patients and their dietary intake of fatty acids were determined. Significantly lower plasma arachidonic, docosahexaenoic, dihomo-gamma-linolenic and...

The secretion of high molecular weight cathepsin B from cultured human liver cancers. (2007)

Ohsawa, Toshiya, Higashi, Toshihiro, Tsuji, Takao

The biochemical characteristics of cathepsin B secreted from cultured human liver cancer cells were examined. The enzyme activity of culture medium against a synthetic substrate,...

Effect of heart rate and myocardial contractile force on coronary resistance. (2007)

Saito, Daiji, Ueeda, Masayuki, Hina, Kazuyoshi, Watanabe, Hirofumi, Mima, Tsutomu, Hasui, Masahiro, ...

The effect of the heart rate and myocardial contractile force on the extravascular resistance to blood flow of the left anterior descending coronary artery (LAD) was evaluated in 15 mongrel dogs...

Phalloidin-induced alterations of bile canaliculi. (2007)

Yamamoto, Kazuhide, Makino, Yasuhiro, Itoshima, Tatsuya, Kobayashi, Toshinari, Tsuji, Takao

Phalloidin, a toxin from the plant Amanita phalloides, irreversibly polymerizes actin filaments and causes cholestasis. Three-dimensional structural changes induced by phalloidin in the bile...

Ultrasonographic characteristics of small hepatocellular carcinoma. (2007)

Higashi, Toshihiro, Tobe, Kazuo, Asano, Ken-ichiro, Ikeda, Hiroshi, Ohsawa, Toshiya, Iwasaki, Yoshiaki, ...

The ultrasonographic characteristics of hepatocellular carcinomas (HCC) were investigated. Four typical features of HCCs, "mosaic internal echo pattern", "halo", "lateral shadow" and "posterior echo...

Three-dimensional arrangement of ductular structures formed by oval cells during hepatocarcinogenesis. (2007)

Makino, Yasuhiro, Yamamoto, Kazuhide, Tsuji, Takao

The three-dimensional arrangement of ductular structures formed by oval cells in rats fed 2-acetylaminofluorene (2-AAF) was studied by scanning electron microscopy (SEM) of biliary tract casts and...

Type IV collagen-degrading enzyme activity in human serum. (2007)

Hashimoto, Noriaki, Kobayashi, Michio, Watanabe, Akiharu, Higashi, Toshiro, Tsuji, Takao

Type IV collagen-degrading enzyme activity was detected in human serum. Serum was preincubated with 4-aminophenylmercuric acetate and trypsin to activate the enzyme prior to assay. Type IV collagen,...

Serum type IV collagen-degrading enzyme in hepatocellular carcinoma with metastasis. (2007)

Hashimoto, Noriaki, Kobayashi, Michio, Tsuji, Takao

The activity of serum type IV collagen-degrading enzyme was measured in 18 patients with hepatocellular carcinoma (HCC). The enzyme activity was significantly higher, in HCC patients with a tumor...

Detection of serum blocking factors and antibodies to the albumin receptor on HBsAG particles in healthy persons and patients with liver diseases. (2007)

Tsuji, Takao, Takahashi, Kenji, Naito, Kunihiko, Nagashima, Hideo

An enzyme-linked immunosorbent assay (ELISA) for the detection of serum blocking factors (BF), or antibodies to the albumin receptor on HBsAg particles, was developed, and its clinical usefulness was...

Albumin receptors on HBsAG particles and subsequent sero-conversion designate HBeAG-positive patients among chronic active liver disease patients. (2007)

Tsuji, Takao

An enzyme-linked immunosorbent assay (ELISA) for the purpose of detecting the albumin receptor on hepatitis B virus surface antigen (HBsAg) particles was developed. Patient sera with moderate to high...

Different expression of Tn and sialyl-Tn antigens between normal and diseased human gastric epithelial cells. (2007)

Yoshida, Atushi, Sotozono, Masaaki, Nakatou, Tatsuaki, Okada, Yoshio, Tsuji, Takao

Thomsen-Friedenreich antigen (T antigen) has been supposed to be a cancer-specific carbohydrate antigen. We have previously shown that one third of the Japanese population normally expressed T...

Relationship of serum markers of hepatitis B and C virus replication in coinfected patients. (2007)

Tsuji, Hideyuki, Shimomura, Hiroyuki, Fujio, Kozo, Wato, Masaki, Kondo, Junichi, Hasui, Toshimi, ...

To evaluate viral interference between hepatitis B and C, we studied coinfected patients serologically and molecular biologically. Twenty-seven patients positive for hepatitis B surface antigen...

Suppressive effects of transforming growth factor-beta1 produced by hepatocellular carcinoma cell lines on interferon-gamma production by peripheral blood mononuclear cells. (2006)

Mouri, Hirokazu, Sakaguchi, Kohsaku, Sawayama, Tomoyuki, Senoh, Tomonori, Ohta, Takeyuki, Nishimura, Mamoru, ...

Transforming growth factor-beta1 (TGF-beta1) exerts potent immunosuppressive effects. In this study, we investigated the potential role of TGF-beta1 produced by hepatocellular carcinoma (HCC) cell...

Effects of pulsing procedure of interleukin-12 in combination with interleukin-2 on the activation of peripheral blood lymphocytes derived from patients with hepatocellular carcinoma. (2006)

Sawayama, Tomoyuki, Sakaguchi, Kohsaku, Senoh, Tomonori, Ohta, Takeyuki, Nishimura, Mamoru, Takaki, Akinobu, ...

In patients with hepatocellular carcinoma (HCC), natural killer (NK) cell activity decreases significantly, and the reduced activity may be associated with the progression of HCC. In this study we...

Mutations in the hepatitis B virus preS2 region and abrogated receptor activity for polymerized human albumin. (2006)

Kondo, Junichi, Shimomura, Hiroyuki, Fujioka, Shin-ichi, Iwasaki, Yoshiaki, Takagi, Shinjiro, Ohnishi, Yasuhiro, ...

The preS2 region of the hepatitis B virus (HBV) has been reported to have human polymerized albumin receptor (PAR) activity, which correlates with viral replication. Here, we studied the genomic...

Identification of a target antigen recognized by a mouse monoclonal antibody to the bile canalicular surface of rat hepatocytes with a random phage display library. (2006)

Ariyoshi, Masanori, MIzuno, Motowo, Morisue, Yoshiko, Shimada, Morizou, Fujita, Shirou, Nasu, Junichirou, ...

We developed a monoclonal antibody (MoAb) (clone 5E8) against an antigen on the bile canalicular membrane of rat hepatocyte. By immunoblotting, MoAb 5E8 detected a band of 110 kD. In this study, we...

Improvements in the measurement of stool decay-accelerating factor in the detection of colorectal cancer. (2006)

Ohya, Shogen, Mizuno, Motowo, Kawada, Mikihiro, Nasu, Junichirou, Okada, Hiroyuki, Shimomura, Hiroyuki, ...

We have previously developed an enzyme-linked immunosorbent assay (ELISA) to measure stool decay-accelerating factor (DAF) and found that stool DAF concentrations were significantly elevated in...

Development of syngeneic monoclonal anti-idiotype antibodies to mouse monoclonal anti-asialoglycoprotein receptor antibody. (2006)

Hirai, Michio, Mizuno, Motowo, Morisue, Yoshiko, Yoshioka, Masao, Shimada, Morizou, Nasu, Junichirou, ...

Anti-idiotype antibodies (Ab2) play an important role in the homeostasis of immune responses and are related to the development and the disease activity of certain autoimmune diseases. The...

Anti-asialoglycoprotein receptor autoantibodies, detected by a capture-immunoassay, are associated with autoimmune liver diseases. (2006)

Yoshioka, Masao, Mizuno, Motowo, Morisue, Yoshiko, Shimada, Morizou, Hirai, Michio, Nasu, Junichirou, ...

In autoimmune chronic active hepatitis (AIH) and primary biliary cirrhosis (PBC), various autoantibodies including anti-asialoglycoprotein receptor (ASGPR) antibodies have been found in patients'...

Effects of zinc deficiency/zinc supplementation on ammonia metabolism in patients with decompensated liver cirrhosis. (2006)

Yoshida, Yasuhiro, Higashi, Toshihiro, Nouso, Kazuhiro, Nakatsukasa, Harushige, Nakamura, Shin-ichiro, Watanabe, Akiharu, ...

Hepatic encephalopathy is one of the major complications in decompensated liver cirrhosis. The current study was conducted to clarify the mechanisms of zinc deficiency in liver cirrhosis and its...

Therapeutic effects of azathioprine in combination with low-dose prednisolone in patients with intractable autoimmune hepatitis type 1. (2006)

Takenami, Tatsuo, Sakaguchi, Kohsaku, Nishimura, Mamoru, Miyake, Yasuhiro, Miyashita, Manabi, Terao, Masako, ...

This study evaluated the effects of azathioprine in combination with low-dose prednisolone in the management of patients with intractable autoimmune hepatitis. Thirteen patients with intractable...

Cytoskeletal inhibitors, anti-adhesion molecule antibodies, and lectins inhibit hepatocyte spheroid formation. (2006)

Nakamura, Masaki, Shinji, Toshiyuki, Ujiike, Kozo, Hirasaki, Shoji, Koide, Norio, Tsuji, Takao

We investigated the role of cytoskeletons, adhesion molecules, membrane-glycosylations, and proteoglycans in forming the shape of adult rat hepatocyte spheroids. Isolated hepatocytes were cultured on...

Cold activation of serum complement in patients with chronic hepatitis C: study on activating pathway and involvement of IgG. (2006)

Ishii, Yasushi, Shimomura, Hiroyuki, Ito, Mamoru, Miyake, Masanobu, Ikeda, Fusao, Miyake, Jiro, ...

It has been documented that the serum complement activities measured by hemolytic assay (CH50) are decreased after storage of sera at a low temperature in some patients with chronic hepatitis C....

Enzyme-Linked Immunoassay for Midkine, and Its Application to Evaluation of Midkine Levels in Developing Mouse Brain and Sera from Patients with Hepatocellular Carcinomas (1996)

Muramatsu, Hisako, Song, Xiao-jun, Koide, Norio, Hada, Hajime, Tsuji, Takao, Kadomatsu, Kenji, ...

Midkine (MK) is a growth factor that promotes neurite outgrowth and survival of neurons, and enhances the plasminogen activator in endothelial cells. A highly sensitive enzyme-linked immunoassay for...

Reperfusion hastens appearance and extent of distribution of type I collagen in infarct zone: immunohistochemical study in rat experimental infarction (1995)

Yamasaki, Satoshi, Kusachi, Shozo, Moritani, Hiroki, Kondo, Jyun, Hirohata, Satoshi, Tamura, Akiko, ...

Background: The effects of reperfusion on time-dependent alteration of type I collagen have not been examined. Objectives: We compared the sequential changes in the appearance and distribution of...

Oxygen metabolism of the hypertrophic right ventricle in open chest dogs (1991)

Saito, Daiji, Tani, Hideki, Kusachi, Shozo, Uchida, Shinji, Ohbayashi, Naotsugu, Marutani, Morio, ...

Study objective — The aim was to investigate oxygen metabolism of the hypertrophic right ventricle in anaesthetised open chest dogs. Design — Right ventricular hypertrophy was induced by right...

Forskolin potentiates myocardial reactive hyperaemia in the open chest dog: the contribution of adenylate cyclase (1989)

HINA, KAZUYOSHI, WATANABE, HIROFUMI, UEEDA, MASAYUKI, YAMADA, NOBUYUKI, SAITO, DAIJI, HARAOKA, SHOICHI, ...

Forskolin, a diterpene, directly stimulates adenylate cyclase and also potentiates receptor mediated stimulation of this enzyme by many stimulatory agonists. We exploited the potentiating effect of...

Antibody to Heat Shock Protein Can Be Used for Early Serological Monitoring of Helicobacter pylori Eradication Treatment

Yunoki, Naoko, Yokota, Kenji, Mizuno, Motowo, Kawahara, Yoshiro, Adachi, Masayasu, Okada, Hiroyuki, ...

Infection with Helicobacter pylori induces humoral immune responses against various antigens of the bacterium. Heat shock proteins (hsps) are immunodominant antigens in various diseases including H....

Induction of Thymus-Derived γδ T Cells by Escherichia coli Enterotoxin B Subunit in Peritoneal Cavities of Mice

Shimizu, Toshiyasu, Sasaki, Keiko, Kato, Michio, Arimitsu, Hideyuki, Ochi, Sadayuki, Shigemori, Naoko, ...

We examined the activation of intraperitoneal T cells in BALB/c mice by the Escherichia coli enterotoxin B subunit, which induced a specific Th2 type of T-cell response to intraperitoneally...

Isolation of a Human Monoclonal Antibody with Strong Neutralizing Activity against Diphtheria Toxin

Kakita, Mai, Takahashi, Tsuyoshi, Komiya, Takako, Iba, Yoshitaka, Tsuji, Takao, Kurosawa, Yoshikazu, ...

We isolated a human monoclonal antibody against diphtheria toxin (DT). It bound to fragment B with a binding activity (Kd) of 3.01 nM. The neutralizing activity assayed by the rabbit skin test was...