Umeda, Mamoru, Yasuda, Tatsuji
We have already developed the liposome immune lysis assay (LILA) for the determination of C-reactive protein (CRP) by employing an inhibition method and a sandwich method. We herein report a new LILA...
Zhang, Daxian, Yasuda, Tatsuji, Yu, Yingyan, Okada, Shigeru
A carboxyfluorescein (CF)-enveloping soybean phosphatidylcholine liposome was used as a model of physicochemical damage of biomembranes. The liposomes were exposed to a metal-chelate complex [2 mM of...
Yonei, Taiji, Watarai, Shinobu, Okada, Yoshio, Yasuda, Tatsuji, Tsuji, Takao
Monoclonal antibodies were raised against urine proteins from diabetic patients. An antibody, YO-2, stained three protein bands with apparent molecular weights of 66, 49, and 36 kDa. These bands were...
Zhao, Dan-Dan, Watarai, Shinobu, Lee, Jin-tae, Kouchi, Shuuichi, Ohmori, Hitishi, Yasuda, Tatsuji
We compared the transfection efficiency of four types of positively charged liposomes composed of (i) N-(α-trimethylammonioacetyl)-didodecyl-D-glutamate chloride (TMAG), dilauroylphosphatidylcholine...
Availability of Liposomes as Drug Carriers to the Brain (2007)
Kobayashi, Kazuko, Han, Mei, Watarai, Shinobu, Yasuda, Tatsuji
Phospholipid vesicles, also known as liposomes, were examined for their ability to act as a drug carrier to the brain. 9-Amino-1,2,3,4-tetrahydroacridine (THA), a centrally acting...
Sensitive detection of ganglioside GD3 on the cell surface using liposome immune lysis assay. (2007)
Kobayashi, Kazuko, Watarai, Shinobu, Yasuda, Tatsuji
We developed a sensitive method for detection of glycosphingolipid (GSL) antigen(s) on the cell surface. As a model of GSL antigen, ganglioside GD3 was used. An IgM monoclonal antibody (DSG-1)...
ARIX and PHOX2B polymorphisms in patients with congenital superior oblique muscle palsy. (2006)
Jiang, Yan, Matsuo, Toshihiko, Fujiwara, Hirotake, Hasebe, Satoshi, Ohtsuki, Hiroshi, Yasuda, Tatsuji
To identify ARIX gene and PHOX2B gene polymorphisms in patients with congenital superior oblique muscle palsy, 3 exons of the ARIX gene and PHOX2B gene were sequenced by genomic DNA amplification...
Akane Kondo, Junko Inagaki, Kazuko Kobayashi, Hideo Tsukamoto, Daisuke Yamamoto, Mikiya Nakatsuka, ...
Laminin-1 is a structural glycoprotein that forms an integral part of the scaffolding of basement membranes, and plays an important role during embryonic development. We have recently demonstrated a...
Akane Kondo, Junko Inagaki, Kazuko Kobayashi, Hideo Tsukamoto, Daisuke Yamamoto, Mikiya Nakatsuka, ...
Laminin-1 is a structural glycoprotein that forms an integral part of the scaffolding of basement membranes, and plays an important role during embryonic development. We have recently demonstrated a...
Antigenic structures recognized by anti-{beta}2-glycoprotein I auto-antibodies (2005)
Kasahara, Hideki, Matsuura, Eiji, Kaihara, Keiko, Yamamoto, Daisuke, Kobayashi, Kazuko, Inagaki, Junko, ...
β2-Glycoprotein I (β2-GPI) is a major antigen for anti-cardiolipin antibodies and their epitopes are cryptic. Conformation of each domain of β2-GPI was optimized from its crystal structure by...
Antigenic structures recognized by anti-{beta}2-glycoprotein I auto-antibodies (2005)
Kasahara, Hideki, Matsuura, Eiji, Kaihara, Keiko, Yamamoto, Daisuke, Kobayashi, Kazuko, Inagaki, Junko, ...
β2-Glycoprotein I (β2-GPI) is a major antigen for anti-cardiolipin antibodies and their epitopes are cryptic. Conformation of each domain of β2-GPI was optimized from its crystal structure by...
An association of IgG anti-laminin-1 autoantibodies with endometriosis in infertile patients (2003)
Inagaki, Junko, Sugiura-Ogasawara, Mayumi, Nomizu, Motoyoshi, Nakatsuka, Mikiya, Ikuta, Katsuo, Suzuki, Nobuharu, ...
BACKGROUND: Laminin‐1, a multifunctional glycoprotein of the basement membrane, is thought to be important in embryogenesis, embryonic implantation, and placentation. We recently showed that...
Matsuura, Eiji, Inagaki, Junko, Kasahara, Hideki, Yamamoto, Daisuke, Atsumi, Tatsuya, Kobayashi, Kazuko, ...
Binding of β2-glycoprotein I (β2-GPI)-dependent anticardiolipin antibodies (aCL) derived from antiphospholipid syndrome (APS) is significantly reduced in aCL ELISA due to loss of the phospholipid...
Watarai, Shinobu, Kushi, Yasunori, Shigeto, Rie, Misawa, Naoko, Eishi, Yoshinobu, Handa, Shizuo, ...
We have established three kinds of monoclonal antibodies against gangliosides containing N-glycolylneuraminic acid (NeuGc) by immunization of BALB/c mice with the purified gangliosides inserted...
Watarai, Shinobu, Kiura, Katsuyuki, Shigeto, Rie, Shibayama, Takuo, Kimura, Ikuro, Yasuda, Tatsuji
Three kinds of anti-GMl monoclonal antibodies, AGM-1, -2, and -3, of the IgM class were produced by the immunization of BALB/c mice with ganglioside GMl inserted into liposomes with Salmonella...
Eiji, Matsuura, Igarashi, Makoto, Igarashi, Yoshiko, Nagae, Hisato, Ichikawa, Kenji, Yasuda, Tatsuji, ...
Human β2-glycoprotein I (β2-GPI) is involved in cardiolipin (CL) binding of anticardiolipin antibodies (aCL) in systemic lupus erythematosus (SLE). We examined the inter-species differences of...
Production of Monoclonal Antibodies Specific for Ganglioside GD3 (1991)
Watarai, Shinobu, Onuma, Misao, Yasuda, Tatsuji
Four kinds of anti-GD3 monoclonal antibodies, DSG-1, -2, -3, and -4, of the IgM class were obtained by the immunization of BALB/c mice with enzootic bovine leukosis tumor tissue-derived ganglioside...
Suzuki, Yasuo, Nishi, Hiroshi, Hidari, Kazuya, Hirabayashi, Yoshio, Matsumoto, Makoto, Kobayashi, Toshiyuki, ...
A new monoclonal antibody (NS24) directed to the N-acetylneuraminylα2-3Galβ1-4GlcNAc residue in type II sugar chain of N-acetylneuraminyllactoneotetraosylceramide [sialylparagloboside,...
Odaka, Chlkako, Utsunomiya, Naoko, Nakanishl, Mamoru, Arata, Yoji, Manabe, Sanae, Yasuda, Tatsuji, ...
Using la antigen-containing llpid vesicles, we investigated by stopped-flow fluorometry the requirements for helper T cell recognition and activation. When azobenzenearsonate-L-tyrosine...
Watarai, Shinobu, Onuma, Misao, Yamamoto, Satoshi, Yasuda, Tatsuji
The effect of galactocerebroside 3′-sulfate (sulfatide) or cholesterol sulfate on syncytium formation induced by bovine immunodeficiency virus (BIV)-infected cells was investigated in vitro....
KANEGASAKI, Shiro, TANAMOTO, Ken-ichi, YASUDA, Tatsuji, HOMMA, J. Yuzuru, MATSUURA, Motohiro, NAKATSUKA, Mitsunobu, ...
To investigate the structure-activity relationships, various biological activities, including pyrogenicity, lethal toxicity, elicitation of Shwartzman reaction, mitogenicity and tumor necrosis factor...
Glycosphingolipids of Normal Bovine and Enzootic Bovine Leukosis Lymph Node Cells (1986)
WATARAI, Shinobu, YASUDA, Tatsuji, ONUMA, Misao, HANDA, Shizuo
We analyzed glycosphingolipids from normal lymph node cells of seven cattle and lymph node cells of eight cattle with enzootic bovine leukosis. The neutral glycosphingolipids and gangliosides were...
HOMMA, J. Yuzuru, MATSUURA, Motohiro, KANEGASAKI, Shiro, KAWAKUBO, Yasuaki, KOJIMA, Yasuhiko, SHIBUKAWA, Nobuyuki, ...
To confirm the revised lipid A structure of Escherichia coli and to establish the structure responsible for its functions, biological activities of the synthetic compounds based on the presented...
Interaction of Hapten-Sensitized Liposomes with Cells Bearing Hapten-Specific Receptors (1983)
HASHIMOTO, Keiichiro, INOUE, Keizo, NOJIMA, Shoshichi, TADAKUMA, Takushi, YASUDA, Tatsuji
The binding of liposomes sensitized with 2,4-dinitrophenyl-6-N-aminocaproylphos-phatidylethanolamine (DNP-Cap-PE) to MOPC-315 cells, which secrete and bear on their surfaces anti-DNP immunoglobulins,...
HASHIMOTO, Keiichiro, INOUE, Keizo, NOJIMA, Shoshichi, TADAKUMA, Takushi, YASUDA, Tatsuji
The relation between the in vitro immunogenicity of phosphatidylcholine liposomes containing 2, 4-dinitrophenyl-6-N-aminocaproylphosphatidylethanolamine (DNP-Cap-PE) as a hapten and the topographical...
Phosphatidylinositol 4,5-bisphosphate stimulates vesicle formation from liposomes by brain cytosol
Kinuta, Masahiro, Yamada, Hiroshi, Abe, Tadashi, Watanabe, Masami, Li, Shun-Ai, Kamitani, Akihiro, ...
As a step toward the elucidation of mechanisms in vesicle budding, a cell-free assay that measures cytosol-induced vesicle generation from liposomes was established. This assay then was used to...
The stimulatory action of amphiphysin on dynamin function is dependent on lipid bilayer curvature
Yoshida, Yumi, Kinuta, Masahiro, Abe, Tadashi, Liang, Shuang, Araki, Kenta, Cremona, Ottavio, ...
Amphiphysin is a major dynamin-binding partner at the synapse; however, its function in fission is unclear. Incubation of large unilamellar liposomes with mice brain cytosol led to massive formation...
Phosphatidylinositol 4,5-bisphosphate stimulates vesicle formation from liposomes by brain cytosol
Kinuta, Masahiro, Yamada, Hiroshi, Abe, Tadashi, Watanabe, Masami, Li, Shun-Ai, Kamitani, Akihiro, ...
As a step toward the elucidation of mechanisms in vesicle budding, a cell-free assay that measures cytosol-induced vesicle generation from liposomes was established. This assay then was used to...
The stimulatory action of amphiphysin on dynamin function is dependent on lipid bilayer curvature
Yoshida, Yumi, Kinuta, Masahiro, Abe, Tadashi, Liang, Shuang, Araki, Kenta, Cremona, Ottavio, ...
Amphiphysin is a major dynamin-binding partner at the synapse; however, its function in fission is unclear. Incubation of large unilamellar liposomes with mice brain cytosol led to massive formation...
Kondo, Akane, Inagaki, Junko, Kobayashi, Kazuko, Tsukamoto, Hideo, Yamamoto, Daisuke, Nakatsuka, Mikiya, ...
Laminin-1 is a structural glycoprotein that forms an integral part of the scaffolding of basement membranes, and plays an important role during embryonic development. We have recently demonstrated a...