Ito, Masahiro, Guffanti, Arthur A., Oudega, Bauke, Krulwich, Terry A.
A 5.9-kb region of the Bacillus subtilis chromosome is transcribed as a single transcript that is predicted to encode seven membrane-spanning proteins. Homologues of the first gene of this operon,...
Ito, Masahiro, Guffanti, Arthur A., Wang, Wei, Krulwich, Terry A.
The Bacillus subtilis mrp (multiple resistance and pH) operon supports Na+ and alkali resistance via an Na+/H+ antiport, as well as cholate efflux and resistance. Among the individual mutants with...
Jin, Jie, Guffanti, Arthur A., Beck, Catherine, Krulwich, Terry A.
A “Tet(L)-12” version of Tet(L), a tetracycline efflux protein with 14 transmembrane segments (TMS), was constructed by deletion of two central TMS. Tet(L)-12 catalyzed Na+/H+ antiport and...
GerN, an Endospore Germination Protein of Bacillus cereus, Is an Na+/H+-K+ Antiporter
Southworth, Thomas W., Guffanti, Arthur A., Moir, Anne, Krulwich, Terry A.
GerN, a Bacillus cereus spore germination protein, exhibits homology to a widely distributed group of putative cation transporters or channel proteins. GerN complemented the Na+-sensitive phenotype...
Wang, Wei, Guffanti, Arthur A., Wei, Yi, Ito, Masahiro, Krulwich, Terry A.
The chromosomally encoded TetA(L) protein of Bacillus subtilis is a multifunctional tetracycline-metal/H+ antiporter that also exhibits monovalent cation/H+ antiport activity and a net K+ uptake...
All of the transmembrane glutamates of Tet(L) are essential for tetracycline (TET) resistance, and E397 has been shown to be essential for all catalytic modes, i.e., TET-Me2+ and Na+ efflux and K+...
Jin, Jie, Guffanti, Arthur A., Bechhofer, David H., Krulwich, Terry A.
The Tet(L) protein encoded in the Bacillus subtilis chromosome and the closely related Tet(K) protein from Staphylococcus aureus plasmids are multifunctional antiporters that have three cytoplasmic...
Wei, Yi, Southworth, Thomas W., Kloster, Hilde, Ito, Masahiro, Guffanti, Arthur A., Moir, Anne, ...
A putative transport protein (Orf9) of alkaliphilic Bacillus pseudofirmus OF4 belongs to a transporter family (CPA-2) of diverse K+ efflux proteins and cation antiporters. Orf9 greatly increased the...
A tenth atp gene and the conserved atpI gene of a Bacillus atp operon have a role in Mg2+ uptake
Hicks, David B., Wang, ZhenXiong, Wei, Yi, Kent, Rebecca, Guffanti, Arthur A., Banciu, Horia, ...
The atp operon of alkaliphilic Bacillus pseudofirmus OF4, as in most prokaryotes, contains the eight structural genes for the F-ATPase (ATP synthase), which are preceded by an atpI gene that encodes...
Ivey, D. Mack, Sturr, Michael G., Krulwich, Terry A., Hicks, David B.
Molecular biological and biochemical studies of the F1F0-ATP synthase of alkaliphilic Bacillus firmus OF4 show that the enzyme used at pH 7.5 and pH 10.5 is a unique product of the atp operon,...
Sobel, Mark E., Wolfson, Ellen B., Krulwich, Terry A.
Arthrobacter pyridinolis cannot grow on glucose as sole carbon source, although the cells possess catabolic enzymes of the Embden-Meyerhof and pentose phosphate pathways as well as a complete...
Ultrastructural Explanation for Snapping Postfission Movements in Arthrobacter crystallopoietes
Krulwich, Terry A., Pate, Jack L.
The ultrastructure of dividing rod-stage cells of Arthrobacter crystallopoietes was examined by electron microscopy. The cell walls consist of two layers. During cell division, the inner layer...
Krulwich, Terry A., Ensign, J. C.
Succinate and several other compounds which induce sphere to rod morphogenesis of A. crystallopoietes were found to suppress both catabolism and assimilation of glucose. Diauxic growth patterns...
Wolfson, Philip J., Krulwich, Terry A.
Growth of Arthrobacter atrocyaneus and A. pyridinolis on certain growth substrates was found to be inhibited by pyruvate and compounds which can be converted to pyruvate. Growth of A. atrocyaneus on...
Krulwich, Terry A., Ensign, Jerald C., Tipper, Donald J., Strominger, Jack L.
Cell walls of Arthrobacter crystallopoietes were prepared from cells grown as spheres and from peptone- and succinate-induced rod stage cells. Undegraded polysaccharide backbones of the...
Krulwich, Terry A., Ensign, Jerald C., Tipper, Donald J., Strominger, Jack L.
Cell walls of Arthrobacter crystallopoietes grown as spheres and as rods were solubilized by treatment with the B enzyme from Chalaropsis, an N-acetylmuramidase. The neutral glycopeptides were then...
Metabolism of d-Fructose by Arthrobacter pyridinolis
Sobel, Mark E., Krulwich, Terry A.
Previous studies showed that Arthrobacter pyridinolis can transport and utilize d-glucose only after prior growth on certain Krebs cycle intermediates. In contrast, we found that d-fructose was taken...
Stimulation of Nitrobacter agilis by Biotin
Krulwich, Terry A., Funk, Helen B.
Krulwich, Terry A. (Goucher College, Baltimore, Md.), and Helen B. Funk. Stimulation of Nitrobacter agilis by biotin. J. Bacteriol. 90:729–733. 1965.—Addition of biotin to nitrite-mineral medium...
Wolfson, Ellen B., Krulwich, Terry A.
Previous studies have shown that Arthrobacter pyridinolis can transport D-fructose or L-rhamnose using either a phosphoenolpyruvate:hexose phosphotransferase (phosphoenolpyruvate:protein...
Ito, Masahiro, Xu, Haoxing, Guffanti, Arthur A., Wei, Yi, Zvi, Lior, Clapham, David E., ...
The prokaryotic voltage-gated Na+ channel, NaChBac, is one of a growing channel superfamily of unknown function. Here we show that NaVBP, the NaChBac homologue encoded by ncbA in alkaliphilic...
Swartz, Talia H., Ito, Masahiro, Hicks, David B., Nuqui, Mark, Guffanti, Arthur A., Krulwich, Terry A.
Mrp catalyzes secondary Na+/H+ antiport and was hypothesized to have an additional primary energization mode. Mrp-dependent complementation of nonfermentative growth of an Escherichia coli...
Liu, Jun, Xue, Yanfen, Wang, Quanhui, Wei, Yi, Swartz, Talia H., Hicks, David B., ...
In extreme alkaliphiles, Na+/H+ antiporters play a central role in the Na+ cycle that supports pH homeostasis, Na+ resistance, solute uptake, and motility. Properties of individual antiporters have...
Terahara, Naoya, Fujisawa, Makoto, Powers, Benjamin, Henkin, Tina M., Krulwich, Terry A., Ito, Masahiro
A stem-loop mutation between ccpA and motP in the Bacillus subtilis ccpA-motPS operon increased motPS transcription and membrane-associated MotPS levels, motility, and number of flagella/cell when...
Ito, Masahiro, Guffanti, Arthur A., Oudega, Bauke, Krulwich, Terry A.
A 5.9-kb region of the Bacillus subtilis chromosome is transcribed as a single transcript that is predicted to encode seven membrane-spanning proteins. Homologues of the first gene of this operon,...
Ito, Masahiro, Guffanti, Arthur A., Wang, Wei, Krulwich, Terry A.
The Bacillus subtilis mrp (multiple resistance and pH) operon supports Na+ and alkali resistance via an Na+/H+ antiport, as well as cholate efflux and resistance. Among the individual mutants with...
Jin, Jie, Guffanti, Arthur A., Beck, Catherine, Krulwich, Terry A.
A “Tet(L)-12” version of Tet(L), a tetracycline efflux protein with 14 transmembrane segments (TMS), was constructed by deletion of two central TMS. Tet(L)-12 catalyzed Na+/H+ antiport and...
GerN, an Endospore Germination Protein of Bacillus cereus, Is an Na+/H+-K+ Antiporter
Southworth, Thomas W., Guffanti, Arthur A., Moir, Anne, Krulwich, Terry A.
GerN, a Bacillus cereus spore germination protein, exhibits homology to a widely distributed group of putative cation transporters or channel proteins. GerN complemented the Na+-sensitive phenotype...
Wang, Wei, Guffanti, Arthur A., Wei, Yi, Ito, Masahiro, Krulwich, Terry A.
The chromosomally encoded TetA(L) protein of Bacillus subtilis is a multifunctional tetracycline-metal/H+ antiporter that also exhibits monovalent cation/H+ antiport activity and a net K+ uptake...
All of the transmembrane glutamates of Tet(L) are essential for tetracycline (TET) resistance, and E397 has been shown to be essential for all catalytic modes, i.e., TET-Me2+ and Na+ efflux and K+...
Jin, Jie, Guffanti, Arthur A., Bechhofer, David H., Krulwich, Terry A.
The Tet(L) protein encoded in the Bacillus subtilis chromosome and the closely related Tet(K) protein from Staphylococcus aureus plasmids are multifunctional antiporters that have three cytoplasmic...
Wei, Yi, Southworth, Thomas W., Kloster, Hilde, Ito, Masahiro, Guffanti, Arthur A., Moir, Anne, ...
A putative transport protein (Orf9) of alkaliphilic Bacillus pseudofirmus OF4 belongs to a transporter family (CPA-2) of diverse K+ efflux proteins and cation antiporters. Orf9 greatly increased the...
A tenth atp gene and the conserved atpI gene of a Bacillus atp operon have a role in Mg2+ uptake
Hicks, David B., Wang, ZhenXiong, Wei, Yi, Kent, Rebecca, Guffanti, Arthur A., Banciu, Horia, ...
The atp operon of alkaliphilic Bacillus pseudofirmus OF4, as in most prokaryotes, contains the eight structural genes for the F-ATPase (ATP synthase), which are preceded by an atpI gene that encodes...
Ivey, D. Mack, Sturr, Michael G., Krulwich, Terry A., Hicks, David B.
Molecular biological and biochemical studies of the F1F0-ATP synthase of alkaliphilic Bacillus firmus OF4 show that the enzyme used at pH 7.5 and pH 10.5 is a unique product of the atp operon,...
Sobel, Mark E., Wolfson, Ellen B., Krulwich, Terry A.
Arthrobacter pyridinolis cannot grow on glucose as sole carbon source, although the cells possess catabolic enzymes of the Embden-Meyerhof and pentose phosphate pathways as well as a complete...
Ultrastructural Explanation for Snapping Postfission Movements in Arthrobacter crystallopoietes
Krulwich, Terry A., Pate, Jack L.
The ultrastructure of dividing rod-stage cells of Arthrobacter crystallopoietes was examined by electron microscopy. The cell walls consist of two layers. During cell division, the inner layer...
Krulwich, Terry A., Ensign, J. C.
Succinate and several other compounds which induce sphere to rod morphogenesis of A. crystallopoietes were found to suppress both catabolism and assimilation of glucose. Diauxic growth patterns...
Wolfson, Philip J., Krulwich, Terry A.
Growth of Arthrobacter atrocyaneus and A. pyridinolis on certain growth substrates was found to be inhibited by pyruvate and compounds which can be converted to pyruvate. Growth of A. atrocyaneus on...
Krulwich, Terry A., Ensign, Jerald C., Tipper, Donald J., Strominger, Jack L.
Cell walls of Arthrobacter crystallopoietes were prepared from cells grown as spheres and from peptone- and succinate-induced rod stage cells. Undegraded polysaccharide backbones of the...
Krulwich, Terry A., Ensign, Jerald C., Tipper, Donald J., Strominger, Jack L.
Cell walls of Arthrobacter crystallopoietes grown as spheres and as rods were solubilized by treatment with the B enzyme from Chalaropsis, an N-acetylmuramidase. The neutral glycopeptides were then...
Metabolism of d-Fructose by Arthrobacter pyridinolis
Sobel, Mark E., Krulwich, Terry A.
Previous studies showed that Arthrobacter pyridinolis can transport and utilize d-glucose only after prior growth on certain Krebs cycle intermediates. In contrast, we found that d-fructose was taken...
Stimulation of Nitrobacter agilis by Biotin
Krulwich, Terry A., Funk, Helen B.
Krulwich, Terry A. (Goucher College, Baltimore, Md.), and Helen B. Funk. Stimulation of Nitrobacter agilis by biotin. J. Bacteriol. 90:729–733. 1965.—Addition of biotin to nitrite-mineral medium...
Wolfson, Ellen B., Krulwich, Terry A.
Previous studies have shown that Arthrobacter pyridinolis can transport D-fructose or L-rhamnose using either a phosphoenolpyruvate:hexose phosphotransferase (phosphoenolpyruvate:protein...
Ito, Masahiro, Xu, Haoxing, Guffanti, Arthur A., Wei, Yi, Zvi, Lior, Clapham, David E., ...
The prokaryotic voltage-gated Na+ channel, NaChBac, is one of a growing channel superfamily of unknown function. Here we show that NaVBP, the NaChBac homologue encoded by ncbA in alkaliphilic...
Swartz, Talia H., Ito, Masahiro, Hicks, David B., Nuqui, Mark, Guffanti, Arthur A., Krulwich, Terry A.
Mrp catalyzes secondary Na+/H+ antiport and was hypothesized to have an additional primary energization mode. Mrp-dependent complementation of nonfermentative growth of an Escherichia coli...
Liu, Jun, Xue, Yanfen, Wang, Quanhui, Wei, Yi, Swartz, Talia H., Hicks, David B., ...
In extreme alkaliphiles, Na+/H+ antiporters play a central role in the Na+ cycle that supports pH homeostasis, Na+ resistance, solute uptake, and motility. Properties of individual antiporters have...
Terahara, Naoya, Fujisawa, Makoto, Powers, Benjamin, Henkin, Tina M., Krulwich, Terry A., Ito, Masahiro
A stem-loop mutation between ccpA and motP in the Bacillus subtilis ccpA-motPS operon increased motPS transcription and membrane-associated MotPS levels, motility, and number of flagella/cell when...
Adaptive Gene Expression in Bacillus subtilis Strains Deleted for tetL
Wei, Yi, Deikus, Gintaras, Powers, Benjamin, Shelden, Victor, Krulwich, Terry A., Bechhofer, David H.
The growth properties of a new panel of Bacillus subtilis tetL deletion strains and of a derivative set of strains in which tetL is restored to the chromosome support earlier indications that...
Swartz, Talia H., Ito, Masahiro, Ohira, Takayuki, Natsui, Shinsuke, Hicks, David B., Krulwich, Terry A.
Monovalent cation proton antiporter-3 (Mrp) family antiporters are widely distributed and physiologically important in prokaryotes. Unlike other antiporters, they require six or seven hydrophobic...
Fujisawa, Makoto, Ito, Masahiro, Krulwich, Terry A.
Properties of four two-component bacterial transport systems of the cation/proton antiporter-2 (CPA2) family led to suggestions that this CPA2 subset may use a channel rather than an antiport...
Morino, Masato, Natsui, Shinsuke, Swartz, Talia H., Krulwich, Terry A., Ito, Masahiro
Mrp antiporters catalyze secondary Na+(Li+)/H+ antiport and/or K+/H+ antiport that is physiologically important in diverse bacteria. An additional capacity for anion flux has been observed for a few...
Terahara, Naoya, Krulwich, Terry A., Ito, Masahiro
Bacterial flagella contain membrane-embedded stators, Mot complexes, that harness the energy of either transmembrane proton or sodium ion gradients to power motility. Use of sodium ion gradients is...