Infection of Nondividing Cells by Rous Sarcoma Virus
Hatziioannou, Theodora, Goff, Stephen P.
A direct comparison demonstrates that Rous sarcoma virus is capable of infecting aphidicolin-arrested cells 10-fold more efficiently than murine leukemia virus but less efficiently than human...
Hatziioannou, Theodora, Valsesia-Wittmann, Sandrine, Russell, Stephen J., Cosset, François-Loïc
We describe retrovirus particles carrying the fowl plague virus (FPV) hemagglutinin (HA). When expressed in cells providing Moloney murine leukemia virus (MoMLV) Gag and Pol proteins and a lacZ...
Cellular inhibitors with Fv1-like activity restrict human and simian immunodeficiency virus tropism
Cowan, Simone, Hatziioannou, Theodora, Cunningham, Tshaka, Muesing, Mark A., Gottlinger, Heinrich G., Bieniasz, Paul D.
Many nonhuman primate cells are unable to support the replication of HIV-1, whereas others are nonpermissive for infection by simian immunodeficiency virus from macaques (SIVmac). Here, we show that...
Zhang, Yi-jun, Hatziioannou, Theodora, Zang, Trinity, Braaten, Douglas, Luban, Jeremy, Goff, Stephen P., ...
Cell surface glycosaminoglycans (GAGs), in particular heparan sulfate (HS), have been proposed to mediate the attachment of human immunodeficiency virus type 1 (HIV-1) to target cells prior to virus...
Restriction of multiple divergent retroviruses by Lv1 and Ref1
Hatziioannou, Theodora, Cowan, Simone, Goff, Stephen P., Bieniasz, Paul D., Towers, Greg J.
The mouse gene Fv1 encodes a saturable restriction factor that selectively blocks infection by N-tropic or B-tropic murine leukemia virus (MLV) strains. Despite the absence of an Fv1 gene, a similar...
Hatziioannou, Theodora, Cowan, Simone, Bieniasz, Paul D.
Retrovirus tropism can be restricted by cellular factors such as Fv1, Ref1, and Lv1 that inhibit infection by targeting the incoming viral capsid. Here, we show that rodent cells exhibit differential...
Species-Specific Tropism Determinants in the Human Immunodeficiency Virus Type 1 Capsid
Hatziioannou, Theodora, Cowan, Simone, Von Schwedler, Uta K., Sundquist, Wesley I., Bieniasz, Paul D.
Retroviral tropism is determined in part by cellular restriction factors that block infection by targeting the incoming viral capsid. Indeed, human immunodeficiency virus type 1 (HIV-1) infection of...
Retrovirus resistance factors Ref1 and Lv1 are species-specific variants of TRIM5α
Hatziioannou, Theodora, Perez-Caballero, David, Yang, Annie, Cowan, Simone, Bieniasz, Paul D.
Mammalian cells express several factors that act in a cell-autonomous manner to inhibit retrovirus replication. Among these are the Friend virus susceptibility factor 1/lentivirus susceptibility...
Perez-Caballero, David, Hatziioannou, Theodora, Martin-Serrano, Juan, Bieniasz, Paul D.
Human immunodeficiency virus type 1 (HIV-1) Gag multimerization and membrane binding are required for particle formation. However, it is unclear what constitutes a minimal plasma membrane-specific...
Hatziioannou, Theodora, Perez-Caballero, David, Cowan, Simone, Bieniasz, Paul D.
Cyclophilin A (CypA) is a peptidyl-prolyl isomerase that binds to the capsid protein (CA) of human immunodeficiency virus type 1 (HIV-1) and by doing so facilitates HIV-1 replication. Although CypA...
Naghavi, Mojgan H., Hatziioannou, Theodora, Gao, Guangxia, Goff, Stephen P.
Two mutant Rat2 fibroblast cell lines, R3-2 and R4-7, have been previously isolated by a selection for retrovirus resistance. We have now further analyzed the basis of the block to retroviral...
Perez-Caballero, David, Hatziioannou, Theodora, Zhang, Fengwen, Cowan, Simone, Bieniasz, Paul D.
TRIM-CypA is an owl monkey-specific variant of the retrovirus restriction factor TRIM5α. Here, we exploit its modular domain organization and cyclosporine sensitivity to probe the kinetics and...
Hatziioannou, Theodora, Martin-Serrano, Juan, Zang, Trinity, Bieniasz, Paul D.
Defective human immunodeficiency virus type 1 (HIV-1) assembly in murine cells is accompanied by poor plasma membrane binding and proteolytic processing of the HIV-1 Gag precursor. Here, we show that...
Human Tripartite Motif 5α Domains Responsible for Retrovirus Restriction Activity and Specificity
Perez-Caballero, David, Hatziioannou, Theodora, Yang, Annie, Cowan, Simone, Bieniasz, Paul D.
The tripartite motif 5α protein (TRIM5α) is one of several factors expressed by mammalian cells that inhibit retrovirus replication. Human TRIM5α (huTRIM5α) inhibits infection by N-tropic murine...
Infection of Nondividing Cells by Rous Sarcoma Virus
Hatziioannou, Theodora, Goff, Stephen P.
A direct comparison demonstrates that Rous sarcoma virus is capable of infecting aphidicolin-arrested cells 10-fold more efficiently than murine leukemia virus but less efficiently than human...
Hatziioannou, Theodora, Valsesia-Wittmann, Sandrine, Russell, Stephen J., Cosset, François-Loïc
We describe retrovirus particles carrying the fowl plague virus (FPV) hemagglutinin (HA). When expressed in cells providing Moloney murine leukemia virus (MoMLV) Gag and Pol proteins and a lacZ...
Cellular inhibitors with Fv1-like activity restrict human and simian immunodeficiency virus tropism
Cowan, Simone, Hatziioannou, Theodora, Cunningham, Tshaka, Muesing, Mark A., Gottlinger, Heinrich G., Bieniasz, Paul D.
Many nonhuman primate cells are unable to support the replication of HIV-1, whereas others are nonpermissive for infection by simian immunodeficiency virus from macaques (SIVmac). Here, we show that...
Zhang, Yi-jun, Hatziioannou, Theodora, Zang, Trinity, Braaten, Douglas, Luban, Jeremy, Goff, Stephen P., ...
Cell surface glycosaminoglycans (GAGs), in particular heparan sulfate (HS), have been proposed to mediate the attachment of human immunodeficiency virus type 1 (HIV-1) to target cells prior to virus...
Restriction of multiple divergent retroviruses by Lv1 and Ref1
Hatziioannou, Theodora, Cowan, Simone, Goff, Stephen P., Bieniasz, Paul D., Towers, Greg J.
The mouse gene Fv1 encodes a saturable restriction factor that selectively blocks infection by N-tropic or B-tropic murine leukemia virus (MLV) strains. Despite the absence of an Fv1 gene, a similar...
Hatziioannou, Theodora, Cowan, Simone, Bieniasz, Paul D.
Retrovirus tropism can be restricted by cellular factors such as Fv1, Ref1, and Lv1 that inhibit infection by targeting the incoming viral capsid. Here, we show that rodent cells exhibit differential...
Species-Specific Tropism Determinants in the Human Immunodeficiency Virus Type 1 Capsid
Hatziioannou, Theodora, Cowan, Simone, Von Schwedler, Uta K., Sundquist, Wesley I., Bieniasz, Paul D.
Retroviral tropism is determined in part by cellular restriction factors that block infection by targeting the incoming viral capsid. Indeed, human immunodeficiency virus type 1 (HIV-1) infection of...
Retrovirus resistance factors Ref1 and Lv1 are species-specific variants of TRIM5α
Hatziioannou, Theodora, Perez-Caballero, David, Yang, Annie, Cowan, Simone, Bieniasz, Paul D.
Mammalian cells express several factors that act in a cell-autonomous manner to inhibit retrovirus replication. Among these are the Friend virus susceptibility factor 1/lentivirus susceptibility...
Perez-Caballero, David, Hatziioannou, Theodora, Martin-Serrano, Juan, Bieniasz, Paul D.
Human immunodeficiency virus type 1 (HIV-1) Gag multimerization and membrane binding are required for particle formation. However, it is unclear what constitutes a minimal plasma membrane-specific...
Hatziioannou, Theodora, Perez-Caballero, David, Cowan, Simone, Bieniasz, Paul D.
Cyclophilin A (CypA) is a peptidyl-prolyl isomerase that binds to the capsid protein (CA) of human immunodeficiency virus type 1 (HIV-1) and by doing so facilitates HIV-1 replication. Although CypA...
Naghavi, Mojgan H., Hatziioannou, Theodora, Gao, Guangxia, Goff, Stephen P.
Two mutant Rat2 fibroblast cell lines, R3-2 and R4-7, have been previously isolated by a selection for retrovirus resistance. We have now further analyzed the basis of the block to retroviral...
Human Tripartite Motif 5α Domains Responsible for Retrovirus Restriction Activity and Specificity
Perez-Caballero, David, Hatziioannou, Theodora, Yang, Annie, Cowan, Simone, Bieniasz, Paul D.
The tripartite motif 5α protein (TRIM5α) is one of several factors expressed by mammalian cells that inhibit retrovirus replication. Human TRIM5α (huTRIM5α) inhibits infection by N-tropic murine...
Perez-Caballero, David, Hatziioannou, Theodora, Zhang, Fengwen, Cowan, Simone, Bieniasz, Paul D.
TRIM-CypA is an owl monkey-specific variant of the retrovirus restriction factor TRIM5α. Here, we exploit its modular domain organization and cyclosporine sensitivity to probe the kinetics and...
Hatziioannou, Theodora, Martin-Serrano, Juan, Zang, Trinity, Bieniasz, Paul D.
Defective human immunodeficiency virus type 1 (HIV-1) assembly in murine cells is accompanied by poor plasma membrane binding and proteolytic processing of the HIV-1 Gag precursor. Here, we show that...
Moesin regulates stable microtubule formation and limits retroviral infection in cultured cells
Naghavi, Mojgan H, Valente, Susana, Hatziioannou, Theodora, De Los Santos, Kenia, Wen, Ying, Mott, Christina, ...
In a functional screen of mammalian complementary DNA libraries, we identified moesin as a novel gene whose overexpression blocks infection by murine leukemia viruses and human immunodeficiency virus...
Antiretroviral Activity and Vif Sensitivity of Rhesus Macaque APOBEC3 Proteins▿
Virgen, Cesar A., Hatziioannou, Theodora
The inability of human immunodeficiency virus type 1(HIV-1) to replicate in rhesus macaque cells is in part due to the failure of HIV-1 Vif to counteract the restriction factor APOBEC3G. However, in...
Independent genesis of chimeric TRIM5-cyclophilin proteins in two primate species
Virgen, Cesar A., Kratovac, Zerina, Bieniasz, Paul D., Hatziioannou, Theodora
The host range of retroviruses is influenced by antiviral proteins such as TRIM5, a restriction factor that recognizes and inactivates incoming retroviral capsids. Remarkably, in Owl monkeys (omk), a...
Primate Lentivirus Capsid Sensitivity to TRIM5 Proteins▿
Kratovac, Zerina, Virgen, Cesar A., Bibollet-Ruche, Frederick, Hahn, Beatrice H., Bieniasz, Paul D., Hatziioannou, Theodora
Mammalian cells express several factors that inhibit lentiviral infection and that have been under strong selective pressure. One of these factors, TRIM5, targets the capsid protein of incoming...
McNatt, Matthew W., Zang, Trinity, Hatziioannou, Theodora, Bartlett, Mackenzie, Fofana, Ismael Ben, Johnson, Welkin E., ...
Tetherin/BST-2/CD317 is a recently identified antiviral protein that blocks the release of nascent retrovirus, and other virus, particles from infected cells. An HIV-1 accessory protein, Vpu, acts as...
Broad-Spectrum Inhibition of Retroviral and Filoviral Particle Release by Tetherin▿
Jouvenet, Nolwenn, Neil, Stuart J. D., Zhadina, Maria, Zang, Trinity, Kratovac, Zerina, Lee, Youngnam, ...
The expression of many putative antiviral genes is upregulated when cells encounter type I interferon (IFN), but the actual mechanisms by which many IFN-induced gene products inhibit virus...
A macaque model of HIV-1 infection
Hatziioannou, Theodora, Ambrose, Zandrea, Chung, Nancy P. Y., Piatak, Michael, Yuan, Fang, Trubey, Charles M., ...
The lack of a primate model that utilizes HIV-1 as the challenge virus is an impediment to AIDS research; existing models generally employ simian viruses that are divergent from HIV-1, reducing their...