Thomas B. Acton

Crystal structure of AGR_C_4470p from Agrobacterium tumefaciens (2007)

Vorobiev, Sergey M., Neely, Helen, Seetharaman, Jayaraman, Ma, Li-Chung, Xiao, Rong, Acton, Thomas B., ...

We report here the crystal structure at 2.0 Å resolution of the AGR_C_4470p protein from the Gram-negative bacterium Agrobacterium tumefaciens. The protein is a tightly associated dimer, each...

Solution structure of Archaeglobus fulgidis peptidyl-tRNA hydrolase (Pth2) provides evidence for an extensive conserved family of Pth2 enzymes in archea, bacteria, and eukaryotes (2005)

Powers, Robert, Mirkovic, Nebojsa, Goldsmith-Fischman, Sharon, Acton, Thomas B., Chiang, Yi-wen, Huang, Yuanpeng J., ...

The solution structure of protein AF2095 from the thermophilic archaea Archaeglobus fulgidis, a 123- residue (13.6-kDa) protein, has been determined by NMR methods. The structure of AF2095 is...

Solution structure of Archaeglobus fulgidis peptidyl-tRNA hydrolase (Pth2) provides evidence for an extensive conserved family of Pth2 enzymes in archea, bacteria, and eukaryotes (2005)

Powers, Robert, Mirkovic, Nebojsa, Goldsmith-Fischman, Sharon, Acton, Thomas B., Chiang, Yiwen, Huang, Yuanpeng J., ...

The solution structure of protein AF2095 from the thermophilic archaea Archaeglobus fulgidis, a 123-residue (13.6-kDa) protein, has been determined by NMR methods. The structure of AF2095 is...

1H, 13C, and 15N assignments for the Archaeglobus fulgidis protein AF2095 (2004)

Powers, Robert, Acton, Thomas B., Chiang, Yi-wen, Rajan, P. K., Cort, John R., Kennedy, Michael A., ...

Structural genomics is providing a means to determine the molecular and cellular function for the vast amount of proteins in the Human proteome that lack any explicit experimental information by...

The NMR solution structure of the 30S ribosomal protein S27e encoded in gene RS27_ARCFU of Archaeoglobus fulgidis reveals a novel protein fold (2004)

Herve Du Penhoat, Catherine, Atreya, Hanudatta S., Shen, Yang, Liu, Gaohua, Acton, Thomas B., Xiao, Rong, ...

The Archaeoglobus fulgidis gene RS27_ARCFU encodes the 30S ribosomal protein S27e. Here, we present the high-quality NMR solution structure of this archaeal protein, which comprises a C4 zinc finger...

Solution NMR structure of the 30S ribosomal protein S28E from Pyrococcus horikoshii (2003)

Aramini, James M., Huang, Yuanpeng J., Cort, John R., Goldsmith-Fischman, Sharon, Xiao, Rong, Shih, Liang-Yu, ...

We report NMR assignments and solution structure of the 71-residue 30S ribosomal protein S28E from the archaean Pyrococcus horikoshii, target JR19 of the Northeast Structural Genomics Consortium. The...

Scanning Mutagenesis of Mcm1: Residues Required for DNA Binding, DNA Bending, and Transcriptional Activation by a MADS-Box Protein

Acton, Thomas B., Mead, Janet, Steiner, Andrew M., Vershon, Andrew K.

MCM1 is an essential gene in the yeast Saccharomyces cerevisiae and is a member of the MADS-box family of transcriptional regulatory factors. To understand the nature of the protein-DNA interactions...

Interactions of the Mcm1 MADS Box Protein with Cofactors That Regulate Mating in Yeast

Mead, Janet, Bruning, Adrian R., Gill, Michael K., Steiner, Andrew M., Acton, Thomas B., Vershon, Andrew K.

The yeast Mcm1 protein is a member of the MADS box family of transcriptional regulatory factors, a class of DNA-binding proteins that control numerous cellular and developmental processes in yeast,...

Structural and biochemical studies identify tobacco SABP2 as a methyl salicylate esterase and implicate it in plant innate immunity

Forouhar, Farhad, Yang, Yue, Kumar, Dhirendra, Chen, Yang, Fridman, Eyal, Park, Sang Wook, ...

Salicylic acid (SA) is a critical signal for the activation of plant defense responses against pathogen infections. We recently identified SA-binding protein 2 (SABP2) from tobacco as a protein that...

Scanning Mutagenesis of Mcm1: Residues Required for DNA Binding, DNA Bending, and Transcriptional Activation by a MADS-Box Protein

Acton, Thomas B., Mead, Janet, Steiner, Andrew M., Vershon, Andrew K.

MCM1 is an essential gene in the yeast Saccharomyces cerevisiae and is a member of the MADS-box family of transcriptional regulatory factors. To understand the nature of the protein-DNA interactions...

Interactions of the Mcm1 MADS Box Protein with Cofactors That Regulate Mating in Yeast

Mead, Janet, Bruning, Adrian R., Gill, Michael K., Steiner, Andrew M., Acton, Thomas B., Vershon, Andrew K.

The yeast Mcm1 protein is a member of the MADS box family of transcriptional regulatory factors, a class of DNA-binding proteins that control numerous cellular and developmental processes in yeast,...

Structural and biochemical studies identify tobacco SABP2 as a methyl salicylate esterase and implicate it in plant innate immunity

Forouhar, Farhad, Yang, Yue, Kumar, Dhirendra, Chen, Yang, Fridman, Eyal, Park, Sang Wook, ...

Salicylic acid (SA) is a critical signal for the activation of plant defense responses against pathogen infections. We recently identified SA-binding protein 2 (SABP2) from tobacco as a protein that...

Molecular insights into substrate recognition and catalysis by tryptophan 2,3-dioxygenase

Forouhar, Farhad, Anderson, J. L. Ross, Mowat, Christopher G., Vorobiev, Sergey M., Hussain, Arif, Abashidze, Mariam, ...

Tryptophan 2,3-dioxygenase (TDO) and indoleamine 2,3-dioxygenase (IDO) constitute an important, yet relatively poorly understood, family of heme-containing enzymes. Here, we report extensive...

Crystal structure of AGR_C_4470p from Agrobacterium tumefaciens

Vorobiev, Sergey M., Neely, Helen, Seetharaman, Jayaraman, Ma, Li-Chung, Xiao, Rong, Acton, Thomas B., ...

We report here the crystal structure at 2.0 Å resolution of the AGR_C_4470p protein from the Gram-negative bacterium Agrobacterium tumefaciens. The protein is a tightly associated dimer, each...

Solution structure of Archaeglobus fulgidis peptidyl-tRNA hydrolase (Pth2) provides evidence for an extensive conserved family of Pth2 enzymes in archea, bacteria, and eukaryotes

Powers, Robert, Mirkovic, Nebojsa, Goldsmith-Fischman, Sharon, Acton, Thomas B., Chiang, Yiwen, Huang, Yuanpeng J., ...

The solution structure of protein AF2095 from the thermophilic archaea Archaeglobus fulgidis, a 123-residue (13.6-kDa) protein, has been determined by NMR methods. The structure of AF2095 is...

The NMR solution structure of the 30S ribosomal protein S27e encoded in gene RS27_ARCFU of Archaeoglobus fulgidis reveals a novel protein fold

Herve Du Penhoat, Catherine, Atreya, Hanudatta S., Shen, Yang, Liu, Gaohua, Acton, Thomas B., Xiao, Rong, ...

The Archaeoglobus fulgidis gene RS27_ARCFU encodes the 30S ribosomal protein S27e. Here, we present the high-quality NMR solution structure of this archaeal protein, which comprises a C4 zinc finger...

Solution NMR structure of the 30S ribosomal protein S28E from Pyrococcus horikoshii

Aramini, James M., Huang, Yuanpeng J., Cort, John R., Goldsmith-Fischman, Sharon, Xiao, Rong, Shih, Liang-Yu, ...

We report NMR assignments and solution structure of the 71-residue 30S ribosomal protein S28E from the archaean Pyrococcus horikoshii, target JR19 of the Northeast Structural Genomics Consortium. The...

Molecular Insights into the Biosynthesis of the F420 Coenzyme*

Forouhar, Farhad, Abashidze, Mariam, Xu, Huimin, Grochowski, Laura L., Seetharaman, Jayaraman, Hussain, Munif, ...

Coenzyme F420, a hydride carrier, is found in Archaea and some bacteria and has crucial roles in methanogenesis, antibiotic biosynthesis, DNA repair, and activation of antitubercular compounds. CofD,...

Structural genomics is the largest contributor of novel structural leverage

Nair, Rajesh, Liu, Jinfeng, Soong, Ta-Tsen, Acton, Thomas B., Everett, John K., Kouranov, Andrei, ...

The Protein Structural Initiative (PSI) at the US National Institutes of Health (NIH) is funding four large-scale centers for structural genomics (SG). These centers systematically target many large...