Thomas F. Meyer

Identification of novel Cyclooxygenase-2-dependent genes in Helicobacter pyloriinfection in vivo (2009)

Walduck, Anna K, Weber, Matthias, Wunder, Christian, Juettner, Stefan, Stolte, Manfred, Vieth, Michael, ...

Abstract Background Helicobacter pylori is a crucial determining factor in the pathogenesis of benign and neoplastic gastric diseases. Cyclooxygenase-2 (Cox-2) is the inducible key enzyme of...

A large-scale chemical modification screen identifies design rules to generate siRNAs with high activity, high stability and low toxicity (2009)

Bramsen, Jesper B., Laursen, Maria B., Nielsen, Anne F., Hansen, Thomas B., Bus, Claus, Langkjær, Niels, ...

The use of chemically synthesized short interfering RNAs (siRNAs) is currently the method of choice to manipulate gene expression in mammalian cell culture, yet improvements of siRNA design is...

A large-scale chemical modification screen identifies design rules to generate siRNAs with high activity, high stability and low toxicity (2009)

Bramsen, Jesper B., Laursen, Maria B., Nielsen, Anne F., Hansen, Thomas B., Bus, Claus, Langkjaer, Niels, ...

The use of chemically synthesized short interfering RNAs (siRNAs) is currently the method of choice to manipulate gene expression in mammalian cell culture, yet improvements of siRNA design is...

Gene Expression Profiles of Chlamydophila pneumoniae during the Developmental Cycle and Iron Depletion–Mediated Persistence (2007)

André P. Mäurer, Adrian Mehlitz, Hans J. Mollenkopf, Thomas F. Meyer

The obligate intracellular, gram-negative bacterium Chlamydophila pneumoniae (Cpn) has impact as a human pathogen. Little is known about changes in the Cpn transcriptome during its biphasic...

Gene expression profiles of Chlamydophila pneumoniae during the developmental cycle and iron depletion-mediated persistence (2007)

Mäurer, André P., Mehlitz, Adrian, Mollenkopf, Hans J., Meyer, Thomas F.

The obligate intracellular, gram-negative bacterium Chlamydophila pneumoniae (Cpn) has impact as a human pathogen. Little is known about changes in the Cpn transcriptome during its biphasic...

Gain and Loss of Multiple Genes During the Evolution of Helicobacter pylori (2005)

Helga Gressmann, Bodo Linz, Rohit Ghai, Klaus-Peter Pleissner, Ralph Schlapbach, Yoshio Yamaoka, ...

Sequence diversity and gene content distinguish most isolates of Helicobacter pylori. Even greater sequence differences differentiate distinct populations of H. pylori from different continents, but...

Gain and loss of multiple genes during the evolution of Helicobacter pylori (2005)

Gressmann, Helga, Linz, Bodo, Ghai, Rohit, Pleissner, Klaus-Peter, Schlapbach, Ralph, Yamaoka, Yoshio, ...

Sequence diversity and gene content distinguish most isolates of Helicobacter pylori. Even greater sequence differences differentiate distinct populations of H. pylori from different continents, but...

Lack of stage-specific proteins in coccoid Helicobacter pylori cells (2004)

Bumann,Dirk, Habibi,Hajar, Kan,Biao, Schmid,Monika, Goosmann,Christian, Brinkmann,Volker, ...

Helicobacter pylori exists in two distinct forms, rod shaped or coccoid, in stomachs of infected patients. Based on in vitro proteome comparisons, there are no detectable coccoid-specific proteins,...

Action and reaction: Chlamydophila pneumoniae proteome alteration in a persistent infection induced by iron deficiency (2004)

Wehrl,Wolfgang, Meyer,Thomas F., Jungblut,Peter R., Muller,Eva-Christina, Szczepek,Agnes J.

Chlamydophila pneumoniae is an obligate intracellular pathogen implicated in a variety of acute and chronic diseases. Long-term infections are associated with a persistent life stage, in which...

Helicobacter pylori vaccine development based on combined subproteome analysis (2004)

Bumann,Dirk, Jungblut,Peter R., Meyer,Thomas F.

Effective vaccines could provide long-term solutions to many important infectious diseases, however, vaccine development has been hampered by the slow identification of protective antigens....

The Helicobacter pylori CagA protein induces tyrosine dephosphorylation of ezrin (2004)

Selbach,Matthias, Moese,Stefan, Backert,Steffen, Jungblut,Peter R., Meyer,Thomas F.

Helicobacter pylori is one of the most wide-spread bacterial pathogens and infects the human stomach to cause diseases, such as gastritis, gastric ulceration, and gastric cancer. A major virulence...

Transcription profiling analysis of the mechanisms of vaccine-induced protection against H-pylori (2004)

Walduck,Anna, Schmitt,Andrea, Lucas,Bernadette, Aebischer,Toni, Meyer,Thomas F.

Development of a vaccine against H. pylori is regarded as desirable alternative to the current antibiotic therapy regimens. Mice immunized with an attenuated recombinant Salmonella typhimurium...

Interaction of Chlamydia trachomatis serovar L2 with the host autophagic pathway (2004)

Al-Younes,Hesham M., Brinkmann,Volker, Meyer,Thomas F.

Chlamydiae are obligate intracellular pathogens that replicate within a membrane-bound compartment (the inclusion) and are associated with important human diseases, such as trachoma, pneumonia, and...

Impact of vector-priming on the immunogenicity of a live recombinant Salmonella enterica serovar typhi Ty21a vaccine expressing urease A and B from Helicobacter pylori in human volunteers (2004)

Metzger,Wolfram G., Mansouri,E., Kronawitter,M, Diescher,Susanne, Soerensen,Meike, Hurwitz,Robert, ...

Orally administered recombinant Salmonella vaccines represent an attractive option for mass vaccination programmes against various infectious diseases. Therefore, it is crucial to gather knowledge...

Helicobacter pylori induces AGS cell motility and elongation via independent signaling pathways (2004)

Moese,Stefan, Selbach,Matthias, Kwok,Terry, Brinkmann,Volker, Konig,Wolfgang, Meyer,Thomas F., ...

Helicobacter pylori induces motogenic and cytoskeletal responses in gastric epithelial cells. We demonstrate that these responses can be induced via independent signaling pathways that often occur in...

Type IV pilus retraction in pathogenic Neisseria is regulated by the PilC proteins (2004)

Morand,Philippe C., Bille,Emmanuelle, Morelle,Sandrine, Eugene,Emmanuel, Beretti,Jean-Luc, Wolfgang,Matthew, ...

Pathogenic Neisseria express type IV pili (tfp), which have been shown to play a central role in the interactions of bacteria with their environment. The regulation of piliation thus constitutes a...

Differential recognition of members of the carcinoembryonic antigen family by Afa/Dr adhesins of diffusely adhering Escherichia coli (Afa/Dr DAEC) (2004)

Berger,Cedric N., Billker,Oliver, Meyer,Thomas F., Servin,Alain L., Kansau,Imad

Little is known about the molecular bases underlying the virulence of diffusely adhering Escherichia coli (DAEC) harbouring the Afa/Dr family of adhesins. These adhesins recognize as receptors the...

Genome-wide analysis of transcriptional hierarchy and feedback regulation in the flagellar system of Helicobacter pylori (2004)

Niehus,Eike, Gressmann,Helga, Ye,Fang, Schlapbach,Ralph, Dehio,Michaela, Dehio,Christoph, ...

The flagellar system of Helicobacter pylori, which comprises more than 40 mostly unclustered genes, is essential for colonization of the human stomach mucosa. In order to elucidate the complex...

Web-accessible proteome databases for microbial research (2004)

Pleissner,Klaus-Peter, Eifert,Till, Buettner,Sven, Schmidt,Frank, Boehme,Martina, Meyer,Thomas F., ...

The analysis of proteomes of biological organisms represents a major challenge of the post-genome era. Classical proteomics combines two-dimensional electrophoresis (2-DE) and mass spectrometry (MS)...

Functional analysis of the cag pathogenicity island in Helicobacter pylori isolates from patients with gastritis, peptic ulcer, and gastric cancer (2004)

Backert,Steffen, Schwarz,Tobias, Miehlke,Stephan, Kirsch,Christian, Sommer,Christian, Kwok,Terry, ...

Helicobacter pylori is the causative agent of a variety of gastric diseases, but the clinical relevance of bacterial virulence factors is still controversial. Virulent strains carrying the cag...

Proteomic and gene profiling approaches to study host responses to bacterial infection (2004)

Walduck,Anna, Rudel,Thomas, Meyer,Thomas F.

Infectious disease is the result of an intimate relationship between the pathogen and host, which involves cross-talk. After an initial flood of mainly descriptive reports on the influence of acute...

Identification of candidate antigens for serologic detection of Helicobacter pylori-infected patients with gastric carcinoma (2004)

Krah,Alexander, Miehlke,Stephan, Pleissner,Klaus-Peter, Zimny-Arndt,Ursula, Kirsch,Christian, Lehn,Norbert, ...

Helicobacter pylori colonizes the stomach of almost half the world population and is a causative agent of gastric carcinomas and duodenal ulcers. Only a small fraction of infected people will develop...

From the inside out - processing of the Chlamydial autotransporter PmpD and its role in bacterial adhesion and activation of human host cells (2004)

Wehrl,Wolfgang, Brinkmann,Volker, Jungblut,Peter R., Meyer,Thomas F., Szczepek,Agnes J.

Summary Polymorphic membrane protein (Pmp)21 otherwise known as PmpD is the longest of 21 Pmps expressed by Chlamydophila pneumoniae. Recent bioinformatical analyses annotated PmpD as belonging to a...

Helicobacter pylori stimulates host vascular endothelial growth factor-A (vegf-A) gene expression via MEK/ERK-dependent activation of Sp1 and Sp3 (2004)

Strowski,Mathias Z., Cramer,Thorsten, Schafer,Georgia, Juttner,Stefan, Walduck,Anna, Schipani,Ernestina, ...

SPECIFIC AIMS Vascular endothelial growth factor-A (VEGF-A) is a key regulator of inflammatory and tumor-associated angiogenesis. Helicobacter pylori (H. pylori) plays a critical role in the...

Autodisplay: Efficacious surface exposure of antigenic UreA fragments from Helicobacter pylori in Salmonella vaccine strains (2003)

Rizos,Konstantin, Lattemann,Claus T., Bumann,Dirk, Meyer,Thomas F., Aebischer,Toni

Live attenuated Salmonella strains expressing antigens of pathogens are promising oral vaccine candidates. There is growing evidence that the topology of expression of the foreign antigens can have a...

Helicobacter pylori stimulates host cyclooxygenase-2 gene transcription: critical importance of MEK/ERK-dependent activation of USF1/-2 and CREB transcription factors (2003)

Juttner,Stefan, Cramer,Thorsten, Wessler,Silja, Walduck,Anna, Gao,Feng, Schmitz,Frank, ...

Cyclooxygenase-2 (COX-2) represents the inducible key enzyme of arachidonic acid metabolism and contributes to the pathogenesis of gastroduodenal ulcers and gastric cancer. Helicobacter pylori...

Expression and translocation of chlamydial protease during acute and persistent infection of the epithelial HEp-2 cells with Chlamydophila (Chlamydia) pneumoniae (2003)

Heuer,Dagmar, Brinkmann,Volker, Meyer,Thomas F., Szczepek,Agnes J.

Summary Chlamydial protease-like activity factor (CPAF) is secreted to the cytoplasm of the infected cells where it proteolytically cleaves eukaryotic transcription factor RFX5. Here, we determined...

The integration site of the iga gene in commensal Neisseria sp. (2003)

Jose,J., Otto,G. W., Meyer,Thomas F.

An IgA1 protease is produced by the human pathogens Neisseria gonorrhoeae and N. meningitidis but not by related non-pathogenic, commensal, Neisseria species. In this study, the chromosomal iga locus...

Helicobacter pylori CagA protein targets the c-Met receptor and enhances the motogenic response (2003)

Churin,Yuri, Al-Ghoul,Laila, Kepp,Oliver, Meyer,Thomas F., Birchmeier,Walter, Naumann,Michael

Infection with the human microbial pathogen Helicobacter pylori is assumed to lead to invasive gastric cancer. We find that H. pylori activates the hepatocyte growth factor/scatter factor receptor...

High throughput RNA interference for gene function analysis in mammalian cells (2003)

Rudel,Thomas, Machuy,Nikolaus, Meyer,Thomas F.

The release of the complete human genome sequence in 2001 marked an important point on the way to the ultimate goal to unravel the function of every protein. The prime technology for investigating...

The Helicobacter pylori CagA protein induces cortactin dephosphorylation and actin rearrangement by c-Src inactivation (2003)

Selbach,Matthias, Moese,Stefan, Hurwitz,Robert, Hauck,Christof R., Meyer,Thomas F., Backert,Steffen

The gastric pathogen Helicobacter pylori translocates the CagA protein into epithelial cells by a type IV secretion process. Translocated CagA is tyrosine phosphorylated (CagA(P-Tyr)) on specific...

'Small' talk: Opa proteins as mediators of Neisseria-host-cell communication (2003)

Hauck,Christof R., Meyer,Thomas F.

Opa proteins are variable outer membrane proteins of Neisseria gonorrhoeae and Neisseria meningitidis that mediate tight interaction of these pathogens with human cells. They have emerged as a...

Integrated, automated system for efficient gene silencing (2003)

Machuy,Nikolaus, Dimmler,Chris, Rudel,Thomas, Meyer,Thomas F., Dennig,Jorg, Ferfers,Claudia, ...

Automated procedures for RNA interference (RNAi) would be valuable tools for high-throughput gene silencing experiments, allowing efficient screening for proteins of interest. We used a range of...

'Small' talk : Opa proteins as mediators of Neisseria-host-cell communication (2003)

Hauck, Christof R., Meyer, Thomas F.

Opa proteins are variable outer membrane proteins of Neisseria gonorrhoeae and Neisseria meningitidis that mediate tight interaction of these pathogens with human cells. They have emerged as a...

Helicobacter pylori type IV secretion, host cell signalling and vaccine development. (2002)

Backert,Steffen, Churin,Yuri, Meyer,Thomas F.

Helicobacter pylori is a bacterial pathogen specialised to colonise and persist the gastric mucosa and to cause severe gastroduodenal disease. A major disease-associated bacterial component is a type...

A comparison of murine and human immunoproteomes of Helicobacter pylori validates the preclinical murine infection model for antigen screening (2002)

Bumann,Dirk, Holland,Petra, Siejak,Frank, Koesling,Jan, Sabarth,Nicolas, Lamer,Stefanie, ...

Preclinical mouse infection models are widely used for Helicobacter vaccine development, but how well such models mimic important aspects of human infections is unknown. A comparison of Helicobacter...

Multiparameter selection of Helicobacter pylori antigens identifies two novel antigens with high protective efficacy (2002)

Sabarth,Nicolas, Hurwitz,Robert, Meyer,Thomas F., Bumann,Dirk

A multiparameter selection of Helicobacter pylori antigens for vaccine development identified 15 candidates, 6 of which are known protective antigens. Two novel antigens with low homology to other...

Identification of surface proteins of Helicobacter pylori by selective biotinylation, affinity purification, and two- dimensional gel electrophoresis (2002)

Sabarth,Nicolas, Lamer,Stefanie, Zimny-Arndt,Ursula, Jungblut,Peter R., Meyer,Thomas F., Bumann,Dirk

Helicobacter pylori is a widespread human pathogen that can cause gastric ulcers and cancer. To identify surface proteins that may play a role in pathogen-host interactions and represent potential...

cag(+) Helicobacter pylori induces homotypic aggregation of macrophage-like cells by up-regulation and recruitment of intracellular adhesion molecule 1 to the cell surface (2002)

Moese,Stefan, Selbach,Matthias, Meyer,Thomas F., Backert,Steffen

Infection with cag(+) but not cag-negative Helicobacter pylori leads to the formation of large homotypic aggregates of macrophage-like cells. Intracellular adhesion molecule I is up- regulated and...

Proteome analysis of secreted proteins of the gastric pathogen Helicobacter pylori (2002)

Bumann,Dirk, Aksu,Sevil, Wendland,Meike, Janek,Katharina, Zimny-Arndt,Ursula, Sabarth,Nicolas, ...

Secreted proteins (the secretome) of the human pathogen Helicobacter pylori may mediate important pathogen-host interactions, but such proteins are technically difficult to analyze. Here, we report...

VDAC and the bacterial porin PorB of Neisseria gonorrhoeae share mitochondrial import pathways (2002)

Muller,Anne, Rassow,Joachim, Grimm,Jan, Machuy,Nikolaus, Meyer,Thomas F., Rudel,Thomas

The human pathogen Neisseria gonorrhoeae induces host cell apoptosis during infection by delivering the outer membrane protein PorB to the host cell's mitochondria. PorB is a pore- forming...

Specific entry of Helicobacter pylori into cultured gastric epithelial cells via a zipper-like mechanism (2002)

Kwok,Terry, Backert,Steffen, Schwarz,Heinz, Berger,Jurgen, Meyer,Thomas F.

Although Helicobacter pylori has generally been considered an extracellular pathogen, a number of in vitro infection experiments and biopsy examinations have shown that it is capable of occasionally...

Src is the kinase of the Helicobacter pylori CagA protein in vitro and in vivo (2002)

Selbach,Matthias, Moese,Stefan, Hauck,Christof R., Meyer,Thomas F., Backert,Steffen

The gastric pathogen Helicobacter pylori uses a type IV secretion system to inject the bacterial CagA protein into gastric epithelial cells. Within the host cell, CagA becomes phosphorylated on...

Nuclear factor-kappa B directs carcinoembryonic antigen-related cellular adhesion molecule 1 receptor expression in Neisseria gonorrhoeae-infected epithelial cells (2002)

Muenzner,Petra, Billker,Oliver, Meyer,Thomas F., Naumann,Michael

The human-specific pathogen Neisseria gonorrhoeae expresses opacity-associated (Opa) protein adhesins that bind to various members of the carcinoembryonic antigen-related cellular adhesion molecule...

B-Raf/Rap1 signaling, but not c-Raf-1/Ras, induces the histidine decarboxylase promoter in Helicobacter pylori infection (2002)

Wessler,Silja, Rapp,Ulf R., Wiedenmann,Bertram, Meyer,Thomas F., Schoneberg,Torsten, Hocker,Michael, ...

Histidine decarboxylase (HDC) is the key enzyme for gastric histamine synthesis, and enhanced HDC expression is critically involved in the pathogenesis of gastric disorders, including gastroduodenal...

Immunoproteomics of Helicobacter pylori infection and relation to gastric disease (2002)

Haas,Gaby, Karaali,Galip, Ebermayer,Karl, Metzger,Wolfram G., Lamer,Stefanie, Zimny-Arndt,Ursula, ...

The Gram negative bacterium Helicobacter pylori is a human pathogen which infects the gastric mucosa and causes an inflammatory process leading to gastritis, ulceration and cancer. A systematic,...

Distinct mechanisms of internalization of Neisseria gonorrhoeae by members of the CEACAM receptor family involving Rac1- and Cdc42-dependent and -independent pathways (2002)

Billker,Oliver, Popp,Andreas, Brinkmann,Volker, Wenig,Gerald, Schneider,Jutta, Caron,Emmanuelle, ...

Opa adhesins of pathogenic Neisseria species target four members of the human carcinoembryonic anti. gen-related cellular adhesion molecule (CEACAM) family. CEACAM receptors mediate...

Functional analysis of the Helicobacter pylori cag pathogenicity island reveals both VirD4-CagA-dependent and VirD4-CagA-independent mechanisms (2002)

Selbach,Matthias, Moese,Stefan, Meyer,Thomas F., Backert,Steffen

The type IV secretion machinery encoded by the cag pathogenicity island (PAI) of Helicobacter pylori has been implicated in a series of host responses during infection. Here, we analyzed the function...

Neisserial immunoglobulin A1 protease induces specific T-cell responses in humans (2002)

Tsirpouchtsidis,Anastasios, Hurwitz,Robert, Brinkmann,Volker, Meyer,Thomas F., Haas,Gaby

We have previously shown that immunoglobulin A1 (IgA1) protease, an exoenzyme of pathogenic neisseriae, can trigger the release of proinflammatory cytokines from human monocytic subpopulations. Here,...

Helicobacter pylori type IV secretion, host cell signalling and vaccine development. (2002)

Backert, Steffen, Churin, Yuri, Meyer, Thomas F.

Helicobacter pylori is a bacterial pathogen specialised to colonise and persist the gastric mucosa and to cause severe gastroduodenal disease. A major disease-associated bacterial component is a type...

The Helicobacter pylori CagA protein induces cortactin dephosphorylation and actin rearrangement by c-Src inactivation (2002)

Selbach, Matthias, Moese, Stefan, Hurwitz, Robert, Hauck, Christof R., Meyer, Thomas F., Backert, Steffen

The gastric pathogen Helicobacter pylori translocates the CagA protein into epithelial cells by a type IV secretion process. Translocated CagA is tyrosine phosphorylated (CagAP-Tyr) on specific EPIYA...

Src Is the Kinase of the Helicobacter pylori CagA Protein in Vitro and in Vivo (2002)

Selbach, Matthias, Moese, Stefan, Hauck, Christof R., Meyer, Thomas F., Backert, Steffen

The gastric pathogen Helicobacter pylori uses a type IV secretion system to inject the bacterial CagA protein into gastric epithelial cells. Within the host cell, CagA becomes phosphorylated on...

Safety and immunogenicity of live recombinant Salmonella enterica serovar Typhi Ty21a expressing urease A and B from Helicobacter pylori in human volunteers (2001)

Bumann,Dirk, Metzger,Wolfram G., Mansouri,E., Palme,O., Wendland,M., Hurwitz,Robert, ...

Helicobacter pylori urease was expressed in the common Eve typhoid vaccine Ty21 a yielding Ty21a(pDB1). Nine volunteers received Ty21a(pDB1) and three control volunteers received Ty21 a. No serious...

PrimeArray: genome-scale primer design for DNA-microarray construction (2001)

Raddatz, Günter, Dehio, Michaela, Meyer, Thomas F., Dehio, Christoph

Summary: PrimeArray is a Windows program that computes oligonuceotide primer pairs for genome-scale gene amplification by the Polymerase Chain Reaction (PCR). The program supports the automated...

Acid sphingomyelinase is involved in CEACAM receptor-mediated phagocytosis of Neisseria gonorrhoeae (2000)

Hauck, Christof R., Grassmé, Heike, Bock, J., Jendrossek, Verena, Ferlinz, Klaus, Meyer, Thomas F., ...

The interaction with human phagocytes is a hallmark of symptomatic Neisseria gonorrhoeae infections. Gonococcal outer membrane proteins of the Opa family induce the opsoninindependent uptake of the...

Tyrosine phosphatase SHP-1 is involved in CD66-mediated phagocytosis of Opa52-expressing Neisseria gonorrhoeae (1999)

Hauck, Christof R., Gulbins, Erich, Lang, Florian, Meyer, Thomas F.

Opa proteins of Neisseria gonorrhoeae bind to CD66 receptors on human phagocytes, thereby inducing efficient uptake of the bacteria in the absence of opsonins. The interaction of Opa proteins and...

CD66-mediated phagocytosis of Opa52 Neisseria gonorrhoeae requires a Src-like tyrosine kinase- and Rac1-dependent signalling pathway (1998)

Hauck, Christof R., Meyer, Thomas F., Lang, Florian, Gulbins, Erich

The interaction of Neisseria gonorrhoeae with human phagocytes is a hallmark of gonococcal infections. Recently, CD66 molecules have been characterized as receptors for Opa52-expressing gonococci on...

The lysosomalphagosomal membrane protein h-lamp-1 is a target of the IgA1 protease of Neisseria gonorrhoeae (1997)

Hauck, Christof R., Meyer, Thomas F.

Lysosomal/phagosomal membranes of mammalian cells are coated by highly conserved glycoproteins (lamps) that are thought to protect the membranes from degradation. Interestingly, we identified an...

An in vitro-differentiated human cell line as a model system to study the interaction of Neisseria gonorrhoeae with phagocytic cells (1997)

Hauck, Christof R., Lorenzen, Dirk, Saas, Joachim, Meyer, Thomas F.

The extreme host specificity of pathogenic neisseriae limits investigations aimed at the analysis of bacterialhost interactions almost completely to the use of in vitro models. Although permanent...

Characterization of the Essential Transport Function of the AIDA-I Autotransporter and Evidence Supporting Structural Predictions

Maurer, Jochen, Jose, Joachim, Meyer, Thomas F.

The current model for autodisplay suggests a mechanism that allows a passenger protein to be translocated across the outer membrane by coordinate action of a C-terminal β-barrel and its preceding...

Autodisplay: Functional Display of Active β-Lactamase on the Surface of Escherichia coli by the AIDA-I Autotransporter

Lattemann, Claus T., Maurer, Jochen, Gerland, Elke, Meyer, Thomas F.

Members of the protein family of immunoglobulin A1 protease-like autotransporters comprise multidomain precursors consisting of a C-terminal autotransporter domain that promotes the translocation of...

Roles of PilC and PilE Proteins in Pilus-Mediated Adherence of Neisseria gonorrhoeae and Neisseria meningitidis to Human Erythrocytes and Endothelial and Epithelial Cells

Scheuerpflug, Ina, Rudel, Thomas, Ryll, Roland, Pandit, Jasmine, Meyer, Thomas F.

Unlike other type 4 pili, the neisserial pili consist of at least two distinct proteins, the highly variable major subunit PilE forming the pilus fiber and the tip-associated adhesin PilC. PilC...

Tyrosine Phosphatase SHP-1 Is Involved in CD66-Mediated Phagocytosis of Opa52-Expressing Neisseria gonorrhoeae

Hauck, Christof R., Gulbins, Erich, Lang, Florian, Meyer, Thomas F.

Opa proteins of Neisseria gonorrhoeae bind to CD66 receptors on human phagocytes, thereby inducing efficient uptake of the bacteria in the absence of opsonins. The interaction of Opa proteins and...

Carcinoembryonic Antigen Family Receptor Specificity of Neisseria meningitidis Opa Variants Influences Adherence to and Invasion of Proinflammatory Cytokine-Activated Endothelial Cells

Muenzner, Petra, Dehio, Christoph, Fujiwara, Taku, Achtman, Mark, Meyer, Thomas F., Gray-Owen, Scott D.

The carcinoembryonic antigen (CEA) family member CEACAM1 (previously called biliary glycoprotein or CD66a) was previously shown to function as a receptor that can mediate the binding of Opa...

Neisseria gonorrhoeae Porin Modifies the Oxidative Burst of Human Professional Phagocytes

Lorenzen, Dirk R., Günther, Dirk, Pandit, Jasmine, Rudel, Thomas, Brandt, Ernst, Meyer, Thomas F.

A hallmark of infection with the gram-negative bacterium Neisseria gonorrhoeae is the local infiltration and subsequent activation of polymorphonuclear neutrophils. Several gonococcal outer membrane...

Immunity against Helicobacter pylori: Significance of Interleukin-4 Receptor α Chain Status and Gender of Infected Mice

Aebischer, Toni, Laforsch, Stephanie, Hurwitz, Robert, Brombacher, Frank, Meyer, Thomas F.

Vaccination of interleukin-4 (IL-4) receptor α (IL-4Rα) chain-deficient BALB/c mice with Helicobacter pylori urease and cholera toxin or with urease-expressing, live attenuated Salmonella enterica...

Adoptive Transfer of CD4+ T Cells Specific for Subunit A of Helicobacter pylori Urease Reduces H. pylori Stomach Colonization in Mice in the Absence of Interleukin-4 (IL-4)/IL-13 Receptor Signaling

Lucas, Bernadette, Bumann, Dirk, Walduck, Anna, Koesling, Jan, Develioglu, Leyla, Meyer, Thomas F., ...

Protection in the murine model of Helicobacter pylori infection may be mediated by CD4+ T cells, but the mechanism remains unclear. To better understand how protection occurs in this model, we...

Helicobacter pylori Resists Phagocytosis by Macrophages: Quantitative Assessment by Confocal Microscopy and Fluorescence-Activated Cell Sorting

Ramarao, Nalini, Meyer, Thomas F.

Helicobacter pylori infection of the stomach epithelium is characterized by an infiltration of polymorphonuclear and mononuclear cells. These immune cells contribute to mucosal damage which may...

Epithelial Cells Infected with Chlamydophila pneumoniae (Chlamydia pneumoniae) Are Resistant to Apoptosis

Rajalingam, Krishnaraj, Al-Younes, Hesham, Müller, Anne, Meyer, Thomas F., Szczepek, Agnes J., Rudel, Thomas

The obligate intracellular pathogen Chlamydophila pneumoniae (Chlamydia pneumoniae) initiates infections in humans via the mucosal epithelia of the respiratory tract. Here, we report that epithelial...

Distinct mechanisms of internalization of Neisseria gonorrhoeae by members of the CEACAM receptor family involving Rac1- and Cdc42-dependent and -independent pathways

Billker, Oliver, Popp, Andreas, Brinkmann, Volker, Wenig, Gerald, Schneider, Jutta, Caron, Emmanuelle, ...

Opa adhesins of pathogenic Neisseria species target four members of the human carcinoembryonic antigen-related cellular adhesion molecule (CEACAM) family. CEACAM receptors mediate...

VDAC and the bacterial porin PorB of Neisseria gonorrhoeae share mitochondrial import pathways

Müller, Anne, Rassow, Joachim, Grimm, Jan, Machuy, Nikolaus, Meyer, Thomas F., Rudel, Thomas

The human pathogen Neisseria gonorrhoeae induces host cell apoptosis during infection by delivering the outer membrane protein PorB to the host cell’s mitochondria. PorB is a pore-forming β-barrel...

Neisserial Immunoglobulin A1 Protease Induces Specific T-Cell Responses in Humans

Tsirpouchtsidis, Anastasios, Hurwitz, Robert, Brinkmann, Volker, Meyer, Thomas F., Haas, Gaby

We have previously shown that immunoglobulin A1 (IgA1) protease, an exoenzyme of pathogenic neisseriae, can trigger the release of proinflammatory cytokines from human monocytic subpopulations. Here,...

Functional Analysis of the Helicobacter pylori cag Pathogenicity Island Reveals Both VirD4-CagA-Dependent and VirD4-CagA-Independent Mechanisms

Selbach, Matthias, Moese, Stefan, Meyer, Thomas F., Backert, Steffen

The type IV secretion machinery encoded by the cag pathogenicity island (PAI) of Helicobacter pylori has been implicated in a series of host responses during infection. Here, we analyzed the function...

Specific Entry of Helicobacter pylori into Cultured Gastric Epithelial Cells via a Zipper-Like Mechanism

Kwok, Terry, Backert, Steffen, Schwarz, Heinz, Berger, Jürgen, Meyer, Thomas F.

Although Helicobacter pylori has generally been considered an extracellular pathogen, a number of in vitro infection experiments and biopsy examinations have shown that it is capable of occasionally...

Proteome Analysis of Secreted Proteins of the Gastric Pathogen Helicobacter pylori

Bumann, Dirk, Aksu, Sevil, Wendland, Meike, Janek, Katharina, Zimny-Arndt, Uschi, Sabarth, Nicolas, ...

Secreted proteins (the secretome) of the human pathogen Helicobacter pylori may mediate important pathogen-host interactions, but such proteins are technically difficult to analyze. Here, we report...

cag+ Helicobacter pylori Induces Homotypic Aggregation of Macrophage-Like Cells by Up-Regulation and Recruitment of Intracellular Adhesion Molecule 1 to the Cell Surface

Moese, Stefan, Selbach, Matthias, Meyer, Thomas F., Backert, Steffen

Infection with cag+ but not cag-negative Helicobacter pylori leads to the formation of large homotypic aggregates of macrophage-like cells. Intracellular adhesion molecule 1 is up-regulated and...

A Comparison of Murine and Human Immunoproteomes of Helicobacter pylori Validates the Preclinical Murine Infection Model for Antigen Screening

Bumann, Dirk, Holland, Petra, Siejak, Frank, Koesling, Jan, Sabarth, Nicolas, Lamer, Stefanie, ...

Preclinical mouse infection models are widely used for Helicobacter vaccine development, but how well such models mimic important aspects of human infections is unknown. A comparison of Helicobacter...

The Helicobacter pylori CagA protein induces cortactin dephosphorylation and actin rearrangement by c-Src inactivation

Selbach, Matthias, Moese, Stefan, Hurwitz, Robert, Hauck, Christof R., Meyer, Thomas F., Backert, Steffen

The gastric pathogen Helicobacter pylori translocates the CagA protein into epithelial cells by a type IV secretion process. Translocated CagA is tyrosine phosphorylated (CagAP-Tyr) on specific...

Autodisplay: Efficacious Surface Exposure of Antigenic UreA Fragments from Helicobacter pylori in Salmonella Vaccine Strains

Rizos, Konstantin, Lattemann, Claus T., Bumann, Dirk, Meyer, Thomas F., Aebischer, Toni

Live attenuated Salmonella strains expressing antigens of pathogens are promising oral vaccine candidates. There is growing evidence that the topology of expression of the foreign antigens can have a...

Targeting of the pro-apoptotic VDAC-like porin (PorB) of Neisseria gonorrhoeae to mitochondria of infected cells

Müller, Anne, Günther, Dirk, Brinkmann, Volker, Hurwitz, Robert, Meyer, Thomas F., Rudel, Thomas

Infection of cell cultures with Neisseria gonorrhoeae results in apoptosis that is mediated by the PorB porin. During the infection process porin translocates from the outer bacterial membrane into...

Functional Analysis of the cag Pathogenicity Island in Helicobacter pylori Isolates from Patients with Gastritis, Peptic Ulcer, and Gastric Cancer

Backert, Steffen, Schwarz, Tobias, Miehlke, Stephan, Kirsch, Christian, Sommer, Christian, Kwok, Terry, ...

Helicobacter pylori is the causative agent of a variety of gastric diseases, but the clinical relevance of bacterial virulence factors is still controversial. Virulent strains carrying the cag...

Type IV pilus retraction in pathogenic Neisseria is regulated by the PilC proteins

Morand, Philippe C, Bille, Emmanuelle, Morelle, Sandrine, Eugène, Emmanuel, Beretti, Jean-Luc, Wolfgang, Matthew, ...

Pathogenic Neisseria express type IV pili (tfp), which have been shown to play a central role in the interactions of bacteria with their environment. The regulation of piliation thus constitutes a...

Helicobacter pylori Induces AGS Cell Motility and Elongation via Independent Signaling Pathways

Moese, Stefan, Selbach, Matthias, Kwok, Terry, Brinkmann, Volker, König, Wolfgang, Meyer, Thomas F., ...

Helicobacter pylori induces motogenic and cytoskeletal responses in gastric epithelial cells. We demonstrate that these responses can be induced via independent signaling pathways that often occur in...

Interaction of Chlamydia trachomatis Serovar L2 with the Host Autophagic Pathway

Al-Younes, Hesham M., Brinkmann, Volker, Meyer, Thomas F.

Chlamydiae are obligate intracellular pathogens that replicate within a membrane-bound compartment (the inclusion) and are associated with important human diseases, such as trachoma, pneumonia, and...

Lack of Stage-Specific Proteins in Coccoid Helicobacter pylori Cells

Bumann, Dirk, Habibi, Hajar, Kan, Biao, Schmid, Monika, Goosmann, Christian, Brinkmann, Volker, ...

Helicobacter pylori exists in two distinct forms, rod shaped or coccoid, in stomachs of infected patients. Based on in vitro proteome comparisons, there are no detectable coccoid-specific proteins,...

CD46-Independent Binding of Neisserial Type IV Pili and the Major Pilus Adhesin, PilC, to Human Epithelial Cells

Kirchner, Marieluise, Heuer, Dagmar, Meyer, Thomas F.

Neisseria gonorrhoeae is a gram-negative bacterial pathogen which infects the human mucosal epithelium. An early critical event in neisserial infection is the type IV pilus-mediated adherence to the...

Analysis of Cell Type-Specific Responses Mediated by the Type IV Secretion System of Helicobacter pylori

Bauer, Bianca, Moese, Stefan, Bartfeld, Sina, Meyer, Thomas F., Selbach, Matthias

Helicobacter pylori persistently infects the human stomach and can cause gastritis, gastric ulceration, and gastric cancer. The type IV secretion system (TFSS) of virulent H. pylori strains...

Gain and Loss of Multiple Genes During the Evolution of Helicobacter pylori

Gressmann, Helga, Linz, Bodo, Ghai, Rohit, Pleissner, Klaus-Peter, Schlapbach, Ralph, Yamaoka, Yoshio, ...

Sequence diversity and gene content distinguish most isolates of Helicobacter pylori. Even greater sequence differences differentiate distinct populations of H. pylori from different continents, but...

Characterization of the ArsRS Regulon of Helicobacter pylori, Involved in Acid Adaptation†

Pflock, Michael, Finsterer, Nadja, Joseph, Biju, Mollenkopf, Hans, Meyer, Thomas F., Beier, Dagmar

The human gastric pathogen Helicobacter pylori is extremely well adapted to the highly acidic conditions encountered in the stomach. The pronounced acid resistance of H. pylori relies mainly on the...

Low-Phosphate-Dependent Invasion Resembles a General Way for Neisseria gonorrhoeae To Enter Host Cells†

Kühlewein, Christiane, Rechner, Cindy, Meyer, Thomas F., Rudel, Thomas

Obligate human-pathogenic Neisseria gonorrhoeae expresses numerous variant surface proteins mediating adherence to and invasion of target cells. The invariant major outer membrane porin PorB of...

Characterization of the Essential Transport Function of the AIDA-I Autotransporter and Evidence Supporting Structural Predictions

Maurer, Jochen, Jose, Joachim, Meyer, Thomas F.

The current model for autodisplay suggests a mechanism that allows a passenger protein to be translocated across the outer membrane by coordinate action of a C-terminal β-barrel and its preceding...

Autodisplay: Functional Display of Active β-Lactamase on the Surface of Escherichia coli by the AIDA-I Autotransporter

Lattemann, Claus T., Maurer, Jochen, Gerland, Elke, Meyer, Thomas F.

Members of the protein family of immunoglobulin A1 protease-like autotransporters comprise multidomain precursors consisting of a C-terminal autotransporter domain that promotes the translocation of...

Roles of PilC and PilE Proteins in Pilus-Mediated Adherence of Neisseria gonorrhoeae and Neisseria meningitidis to Human Erythrocytes and Endothelial and Epithelial Cells

Scheuerpflug, Ina, Rudel, Thomas, Ryll, Roland, Pandit, Jasmine, Meyer, Thomas F.

Unlike other type 4 pili, the neisserial pili consist of at least two distinct proteins, the highly variable major subunit PilE forming the pilus fiber and the tip-associated adhesin PilC. PilC...

Tyrosine Phosphatase SHP-1 Is Involved in CD66-Mediated Phagocytosis of Opa52-Expressing Neisseria gonorrhoeae

Hauck, Christof R., Gulbins, Erich, Lang, Florian, Meyer, Thomas F.

Opa proteins of Neisseria gonorrhoeae bind to CD66 receptors on human phagocytes, thereby inducing efficient uptake of the bacteria in the absence of opsonins. The interaction of Opa proteins and...

Carcinoembryonic Antigen Family Receptor Specificity of Neisseria meningitidis Opa Variants Influences Adherence to and Invasion of Proinflammatory Cytokine-Activated Endothelial Cells

Muenzner, Petra, Dehio, Christoph, Fujiwara, Taku, Achtman, Mark, Meyer, Thomas F., Gray-Owen, Scott D.

The carcinoembryonic antigen (CEA) family member CEACAM1 (previously called biliary glycoprotein or CD66a) was previously shown to function as a receptor that can mediate the binding of Opa...

Neisseria gonorrhoeae Porin Modifies the Oxidative Burst of Human Professional Phagocytes

Lorenzen, Dirk R., Günther, Dirk, Pandit, Jasmine, Rudel, Thomas, Brandt, Ernst, Meyer, Thomas F.

A hallmark of infection with the gram-negative bacterium Neisseria gonorrhoeae is the local infiltration and subsequent activation of polymorphonuclear neutrophils. Several gonococcal outer membrane...

Immunity against Helicobacter pylori: Significance of Interleukin-4 Receptor α Chain Status and Gender of Infected Mice

Aebischer, Toni, Laforsch, Stephanie, Hurwitz, Robert, Brombacher, Frank, Meyer, Thomas F.

Vaccination of interleukin-4 (IL-4) receptor α (IL-4Rα) chain-deficient BALB/c mice with Helicobacter pylori urease and cholera toxin or with urease-expressing, live attenuated Salmonella enterica...

Adoptive Transfer of CD4+ T Cells Specific for Subunit A of Helicobacter pylori Urease Reduces H. pylori Stomach Colonization in Mice in the Absence of Interleukin-4 (IL-4)/IL-13 Receptor Signaling

Lucas, Bernadette, Bumann, Dirk, Walduck, Anna, Koesling, Jan, Develioglu, Leyla, Meyer, Thomas F., ...

Protection in the murine model of Helicobacter pylori infection may be mediated by CD4+ T cells, but the mechanism remains unclear. To better understand how protection occurs in this model, we...

Helicobacter pylori Resists Phagocytosis by Macrophages: Quantitative Assessment by Confocal Microscopy and Fluorescence-Activated Cell Sorting

Ramarao, Nalini, Meyer, Thomas F.

Helicobacter pylori infection of the stomach epithelium is characterized by an infiltration of polymorphonuclear and mononuclear cells. These immune cells contribute to mucosal damage which may...

Epithelial Cells Infected with Chlamydophila pneumoniae (Chlamydia pneumoniae) Are Resistant to Apoptosis

Rajalingam, Krishnaraj, Al-Younes, Hesham, Müller, Anne, Meyer, Thomas F., Szczepek, Agnes J., Rudel, Thomas

The obligate intracellular pathogen Chlamydophila pneumoniae (Chlamydia pneumoniae) initiates infections in humans via the mucosal epithelia of the respiratory tract. Here, we report that epithelial...

Distinct mechanisms of internalization of Neisseria gonorrhoeae by members of the CEACAM receptor family involving Rac1- and Cdc42-dependent and -independent pathways

Billker, Oliver, Popp, Andreas, Brinkmann, Volker, Wenig, Gerald, Schneider, Jutta, Caron, Emmanuelle, ...

Opa adhesins of pathogenic Neisseria species target four members of the human carcinoembryonic antigen-related cellular adhesion molecule (CEACAM) family. CEACAM receptors mediate...

VDAC and the bacterial porin PorB of Neisseria gonorrhoeae share mitochondrial import pathways

Müller, Anne, Rassow, Joachim, Grimm, Jan, Machuy, Nikolaus, Meyer, Thomas F., Rudel, Thomas

The human pathogen Neisseria gonorrhoeae induces host cell apoptosis during infection by delivering the outer membrane protein PorB to the host cell’s mitochondria. PorB is a pore-forming β-barrel...

Neisserial Immunoglobulin A1 Protease Induces Specific T-Cell Responses in Humans

Tsirpouchtsidis, Anastasios, Hurwitz, Robert, Brinkmann, Volker, Meyer, Thomas F., Haas, Gaby

We have previously shown that immunoglobulin A1 (IgA1) protease, an exoenzyme of pathogenic neisseriae, can trigger the release of proinflammatory cytokines from human monocytic subpopulations. Here,...

Functional Analysis of the Helicobacter pylori cag Pathogenicity Island Reveals Both VirD4-CagA-Dependent and VirD4-CagA-Independent Mechanisms

Selbach, Matthias, Moese, Stefan, Meyer, Thomas F., Backert, Steffen

The type IV secretion machinery encoded by the cag pathogenicity island (PAI) of Helicobacter pylori has been implicated in a series of host responses during infection. Here, we analyzed the function...

Specific Entry of Helicobacter pylori into Cultured Gastric Epithelial Cells via a Zipper-Like Mechanism

Kwok, Terry, Backert, Steffen, Schwarz, Heinz, Berger, Jürgen, Meyer, Thomas F.

Although Helicobacter pylori has generally been considered an extracellular pathogen, a number of in vitro infection experiments and biopsy examinations have shown that it is capable of occasionally...

Proteome Analysis of Secreted Proteins of the Gastric Pathogen Helicobacter pylori

Bumann, Dirk, Aksu, Sevil, Wendland, Meike, Janek, Katharina, Zimny-Arndt, Uschi, Sabarth, Nicolas, ...

Secreted proteins (the secretome) of the human pathogen Helicobacter pylori may mediate important pathogen-host interactions, but such proteins are technically difficult to analyze. Here, we report...

cag+ Helicobacter pylori Induces Homotypic Aggregation of Macrophage-Like Cells by Up-Regulation and Recruitment of Intracellular Adhesion Molecule 1 to the Cell Surface

Moese, Stefan, Selbach, Matthias, Meyer, Thomas F., Backert, Steffen

Infection with cag+ but not cag-negative Helicobacter pylori leads to the formation of large homotypic aggregates of macrophage-like cells. Intracellular adhesion molecule 1 is up-regulated and...

A Comparison of Murine and Human Immunoproteomes of Helicobacter pylori Validates the Preclinical Murine Infection Model for Antigen Screening

Bumann, Dirk, Holland, Petra, Siejak, Frank, Koesling, Jan, Sabarth, Nicolas, Lamer, Stefanie, ...

Preclinical mouse infection models are widely used for Helicobacter vaccine development, but how well such models mimic important aspects of human infections is unknown. A comparison of Helicobacter...

The Helicobacter pylori CagA protein induces cortactin dephosphorylation and actin rearrangement by c-Src inactivation

Selbach, Matthias, Moese, Stefan, Hurwitz, Robert, Hauck, Christof R., Meyer, Thomas F., Backert, Steffen

The gastric pathogen Helicobacter pylori translocates the CagA protein into epithelial cells by a type IV secretion process. Translocated CagA is tyrosine phosphorylated (CagAP-Tyr) on specific...

Autodisplay: Efficacious Surface Exposure of Antigenic UreA Fragments from Helicobacter pylori in Salmonella Vaccine Strains

Rizos, Konstantin, Lattemann, Claus T., Bumann, Dirk, Meyer, Thomas F., Aebischer, Toni

Live attenuated Salmonella strains expressing antigens of pathogens are promising oral vaccine candidates. There is growing evidence that the topology of expression of the foreign antigens can have a...

Targeting of the pro-apoptotic VDAC-like porin (PorB) of Neisseria gonorrhoeae to mitochondria of infected cells

Müller, Anne, Günther, Dirk, Brinkmann, Volker, Hurwitz, Robert, Meyer, Thomas F., Rudel, Thomas

Infection of cell cultures with Neisseria gonorrhoeae results in apoptosis that is mediated by the PorB porin. During the infection process porin translocates from the outer bacterial membrane into...

Functional Analysis of the cag Pathogenicity Island in Helicobacter pylori Isolates from Patients with Gastritis, Peptic Ulcer, and Gastric Cancer

Backert, Steffen, Schwarz, Tobias, Miehlke, Stephan, Kirsch, Christian, Sommer, Christian, Kwok, Terry, ...

Helicobacter pylori is the causative agent of a variety of gastric diseases, but the clinical relevance of bacterial virulence factors is still controversial. Virulent strains carrying the cag...

Type IV pilus retraction in pathogenic Neisseria is regulated by the PilC proteins

Morand, Philippe C, Bille, Emmanuelle, Morelle, Sandrine, Eugène, Emmanuel, Beretti, Jean-Luc, Wolfgang, Matthew, ...

Pathogenic Neisseria express type IV pili (tfp), which have been shown to play a central role in the interactions of bacteria with their environment. The regulation of piliation thus constitutes a...

Helicobacter pylori Induces AGS Cell Motility and Elongation via Independent Signaling Pathways

Moese, Stefan, Selbach, Matthias, Kwok, Terry, Brinkmann, Volker, König, Wolfgang, Meyer, Thomas F., ...

Helicobacter pylori induces motogenic and cytoskeletal responses in gastric epithelial cells. We demonstrate that these responses can be induced via independent signaling pathways that often occur in...

Interaction of Chlamydia trachomatis Serovar L2 with the Host Autophagic Pathway

Al-Younes, Hesham M., Brinkmann, Volker, Meyer, Thomas F.

Chlamydiae are obligate intracellular pathogens that replicate within a membrane-bound compartment (the inclusion) and are associated with important human diseases, such as trachoma, pneumonia, and...

Lack of Stage-Specific Proteins in Coccoid Helicobacter pylori Cells

Bumann, Dirk, Habibi, Hajar, Kan, Biao, Schmid, Monika, Goosmann, Christian, Brinkmann, Volker, ...

Helicobacter pylori exists in two distinct forms, rod shaped or coccoid, in stomachs of infected patients. Based on in vitro proteome comparisons, there are no detectable coccoid-specific proteins,...

CD46-Independent Binding of Neisserial Type IV Pili and the Major Pilus Adhesin, PilC, to Human Epithelial Cells

Kirchner, Marieluise, Heuer, Dagmar, Meyer, Thomas F.

Neisseria gonorrhoeae is a gram-negative bacterial pathogen which infects the human mucosal epithelium. An early critical event in neisserial infection is the type IV pilus-mediated adherence to the...

Analysis of Cell Type-Specific Responses Mediated by the Type IV Secretion System of Helicobacter pylori

Bauer, Bianca, Moese, Stefan, Bartfeld, Sina, Meyer, Thomas F., Selbach, Matthias

Helicobacter pylori persistently infects the human stomach and can cause gastritis, gastric ulceration, and gastric cancer. The type IV secretion system (TFSS) of virulent H. pylori strains...

Gain and Loss of Multiple Genes During the Evolution of Helicobacter pylori

Gressmann, Helga, Linz, Bodo, Ghai, Rohit, Pleissner, Klaus-Peter, Schlapbach, Ralph, Yamaoka, Yoshio, ...

Sequence diversity and gene content distinguish most isolates of Helicobacter pylori. Even greater sequence differences differentiate distinct populations of H. pylori from different continents, but...

Characterization of the ArsRS Regulon of Helicobacter pylori, Involved in Acid Adaptation†

Pflock, Michael, Finsterer, Nadja, Joseph, Biju, Mollenkopf, Hans, Meyer, Thomas F., Beier, Dagmar

The human gastric pathogen Helicobacter pylori is extremely well adapted to the highly acidic conditions encountered in the stomach. The pronounced acid resistance of H. pylori relies mainly on the...

Low-Phosphate-Dependent Invasion Resembles a General Way for Neisseria gonorrhoeae To Enter Host Cells†

Kühlewein, Christiane, Rechner, Cindy, Meyer, Thomas F., Rudel, Thomas

Obligate human-pathogenic Neisseria gonorrhoeae expresses numerous variant surface proteins mediating adherence to and invasion of target cells. The invariant major outer membrane porin PorB of...

Gene Expression Profiles of Chlamydophila pneumoniae during the Developmental Cycle and Iron Depletion–Mediated Persistence

Mäurer, André P, Mehlitz, Adrian, Mollenkopf, Hans J, Meyer, Thomas F

The obligate intracellular, gram-negative bacterium Chlamydophila pneumoniae (Cpn) has impact as a human pathogen. Little is known about changes in the Cpn transcriptome during its biphasic...

The Orphan Response Regulator HP1021 of Helicobacter pylori Regulates Transcription of a Gene Cluster Presumably Involved in Acetone Metabolism▿ †

Pflock, Michael, Bathon, Melanie, Schär, Jennifer, Müller, Stefanie, Mollenkopf, Hans, Meyer, Thomas F., ...

Helicobacter pylori is a gastric pathogen for which no nonhuman reservoir is known. In accordance with the tight adaptation to its unique habitat, the human stomach, H. pylori is endowed with a very...

Immunoglobulin A1 Protease, an Exoenzyme of Pathogenic Neisseriae, Is a Potent Inducer of Proinflammatory Cytokines

Lorenzen, Dirk R., Düx, Frank, Wölk, Uwe, Tsirpouchtsidis, Anastasios, Haas, Gaby, Meyer, Thomas F.

A characteristic of human pathogenic Neisseriae is the production and secretion of an immunoglobulin (Ig)A1-specific serine protease (IgA1 protease) that cleaves preferentially human IgA1 and other...

Neisseria gonorrhoeae Epithelial Cell Interaction Leads to the Activation of the Transcription Factors Nuclear Factor κB and Activator Protein 1 and the Induction of Inflammatory Cytokines

Naumann, Michael, Weßler, Silja, Bartsch, Cornelia, Wieland, Björn, Meyer, Thomas F.

We have studied the effect of human bacterial pathogen Neisseria gonorrhoeae (Ngo) on the activation of nuclear factor (NF)-κB and the transcriptional activation of inflammatory cytokine genes upon...

Coordinate Activation of Activator Protein 1 and Inflammatory Cytokines in Response to Neisseria gonorrhoeae Epithelial Cell Contact Involves Stress Response Kinases

Naumann, Michael, Rudel, Thomas, Wieland, Björn, Bartsch, Cornelia, Meyer, Thomas F.

Neisseria gonorrhoeae (Ngo), the etiologic agent of gonorrhea, induce a number of proinflammatory cytokines by contact to epithelial cells. Cytokine genes and a variety of other immune response genes...

Helicobacter pylori CagA protein targets the c-Met receptor and enhances the motogenic response

Churin, Yuri, Al-Ghoul, Laila, Kepp, Oliver, Meyer, Thomas F., Birchmeier, Walter, Naumann, Michael

Infection with the human microbial pathogen Helicobacter pylori is assumed to lead to invasive gastric cancer. We find that H. pylori activates the hepatocyte growth factor/scatter factor receptor...

Competitive Inhibition of Amino Acid Uptake Suppresses Chlamydial Growth: Involvement of the Chlamydial Amino Acid Transporter BrnQ▿ †

Braun, Peter R., Al-Younes, Hesham, Gussmann, Joscha, Klein, Jeannette, Schneider, Erwin, Meyer, Thomas F.

Chlamydiaceae are obligate intracellular bacterial pathogens that strictly depend on host metabolites, such as nucleotides, lipids, and amino acids. Depletion of amino acids in cell culture media...

The Autodisplay Story, from Discovery to Biotechnical and Biomedical Applications

Jose, Joachim, Meyer, Thomas F.

Summary: Among the pathways used by gram-negative bacteria for protein secretion, the autotransporter pathway represents a solution of impressive simplicity. Proteins are transported, independent of...

IFN-γ-Inducible Irga6 Mediates Host Resistance against Chlamydia trachomatis via Autophagy

Al-Zeer, Munir A., Al-Younes, Hesham M., Braun, Peter R., Zerrahn, Jens, Meyer, Thomas F.

Chlamydial infection of the host cell induces Gamma interferon (IFNγ), a central immunoprotector for humans and mice. The primary defense against Chlamydia infection in the mouse involves the...

A large-scale chemical modification screen identifies design rules to generate siRNAs with high activity, high stability and low toxicity

Bramsen, Jesper B., Laursen, Maria B., Nielsen, Anne F., Hansen, Thomas B., Bus, Claus, Langkjær, Niels, ...

The use of chemically synthesized short interfering RNAs (siRNAs) is currently the method of choice to manipulate gene expression in mammalian cell culture, yet improvements of siRNA design is...

Neisseria meningitidis Differentially Controls Host Cell Motility through PilC1 and PilC2 Components of Type IV Pili

Morand, Philippe C., Drab, Marek, Rajalingam, Krishnaraj, Nassif, Xavier, Meyer, Thomas F.

Neisseria meningitidis is a strictly human pathogen that has two facets since asymptomatic carriage can unpredictably turn into fulminant forms of infection. Meningococcal pathogenesis relies on the...

Rab6 and Rab11 Regulate Chlamydia trachomatis Development and Golgin-84-Dependent Golgi Fragmentation

Lipinski, Anette Rejman, Heymann, Julia, Meissner, Charlotte, Karlas, Alexander, Brinkmann, Volker, Meyer, Thomas F., ...

Many intracellular pathogens that replicate in special membrane bound compartments exploit cellular trafficking pathways by targeting small GTPases, including Rab proteins. Members of the...