Thomas G. Ksiazek

Chloroquine is a potent inhibitor of SARS coronavirus infection and spread (2005)

Vincent, Martin J, Bergeron, Eric, Benjannet, Suzanne, Erickson, Bobbie R, Rollin, Pierre E, Ksiazek, Thomas G, ...

Abstract Background Severe acute respiratory syndrome (SARS) is caused by a newly discovered coronavirus (SARS-CoV). No effective prophylactic or post-exposure therapy is currently available. Results...

Viral Discovery and Sequence Recovery Using DNA Microarrays (2003)

David Wang, Anatoly Urisman, Yu-Tsueng Liu, Michael Springer, Thomas G. Ksiazek, Dean D. Erdman, ...

Know your enemy. Description of the ‘virus gene chip’ that helped to classify the SARS virus as a novel coronavirus.

Viral Discovery and Sequence Recovery Using DNA Microarrays (2003)

David Wang, Anatoly Urisman, Yu-Tsueng Liu, Michael Springer, Thomas G. Ksiazek, Dean D. Erdman, ...

Because of the constant threat posed by emerging infectious diseases and the limitations of existing approaches used to identify new pathogens, there is a great demand for new technological methods...

Anticorpos anti-hantavírus em escolares de Salvador, Bahia (1998)

Mascarenhas-Batista,Ana Veronica, Ksiazek,Thomas G., Leduc,James W., Pinheiro,Francisco, ...

As hantaviroses são doenças emergentes nas Américas e, em todo o mundo, os casos clínicos descritos foram infreqüentes entre crianças. O objetivo de investigar a freqüência de escolares...

HANTA VIRUSES AND THEIR RODENT RESERVOIRS IN THE UNITED STATES (1994)

Childs, James E., Ksiazek, Thomas G., Rollesf, Pierre E., Krebs, John W., Zaki, Sherif, Nichol, Stuart T., ...

Since 1993, three novel hantaviruses have been identified from the United States of which at least two can cause a severe respiratory disease termed hantavirus pulmonary syndrome (HPS). Rodent...

Enzyme-Linked Immunosorbent Assays for Detection of Antibodies to Ebola and Marburg Viruses Using Recombinant Nucleoproteins

Saijo, Masayuki, Niikura, Masahiro, Morikawa, Shigeru, Ksiazek, Thomas G., Meyer, Richard F., Peters, Clarence J., ...

The full-length nucleoprotein (NP) of Ebola virus (EBO) was expressed as a His-tagged recombinant protein (His-EBO-NP) by a baculovirus system. Carboxy-terminal halves of NPs of EBO and Marburg virus...

Genetic Analysis of the Diversity and Origin of Hantaviruses in Peromyscus leucopus Mice in North America

Morzunov, Sergey P., Rowe, Joan E., Ksiazek, Thomas G., Peters, Clarence J., St. Jeor, Stephen C., Nichol, Stuart T.

Nucleotide sequences were determined for the complete M genome segments of two distinct hantavirus genetic lineages which were detected in hantavirus antibody- and PCR-positive white-footed mice...

Genetic Diversity among Lassa Virus Strains

Bowen, Michael D., Rollin, Pierre E., Ksiazek, Thomas G., Hustad, Heather L., Bausch, Daniel G., Demby, Austin H., ...

The arenavirus Lassa virus causes Lassa fever, a viral hemorrhagic fever that is endemic in the countries of Nigeria, Sierra Leone, Liberia, and Guinea and perhaps elsewhere in West Africa. To...

Satellite imagery characterizes local animal reservoir populations of Sin Nombre virus in the southwestern United States

Glass, Gregory E., Yates, Terry L., Fine, Joshua B., Shields, Timothy M., Kendall, John B., Hope, Andrew G., ...

The relationship between the risk of hantaviral pulmonary syndrome (HPS), as estimated from satellite imagery, and local rodent populations was examined. HPS risk, predicted before rodent sampling,...

Antibody-Dependent Enhancement of Ebola Virus Infection

Takada, Ayato, Feldmann, Heinz, Ksiazek, Thomas G., Kawaoka, Yoshihiro

Most strains of Ebola virus cause a rapidly fatal hemorrhagic disease in humans, yet there are still no biologic explanations that adequately account for the extreme virulence of these emerging...

Viral Discovery and Sequence Recovery Using DNA Microarrays

Wang, David, Urisman, Anatoly, Liu, Yu-Tsueng, Springer, Michael, Ksiazek, Thomas G, Erdman, Dean D, ...

Because of the constant threat posed by emerging infectious diseases and the limitations of existing approaches used to identify new pathogens, there is a great demand for new technological methods...

Rapid Diagnosis of Ebola Hemorrhagic Fever by Reverse Transcription-PCR in an Outbreak Setting and Assessment of Patient Viral Load as a Predictor of Outcome

Towner, Jonathan S., Rollin, Pierre E., Bausch, Daniel G., Sanchez, Anthony, Crary, Sharon M., Vincent, Martin, ...

The largest outbreak on record of Ebola hemorrhagic fever (EHF) occurred in Uganda from August 2000 to January 2001. The outbreak was centered in the Gulu district of northern Uganda, with secondary...

Marburgvirus Genomics and Association with a Large Hemorrhagic Fever Outbreak in Angola

Towner, Jonathan S., Khristova, Marina L., Sealy, Tara K., Vincent, Martin J., Erickson, Bobbie R., Bawiec, Darcy A., ...

In March 2005, the Centers for Disease Control and Prevention (CDC) investigated a large hemorrhagic fever (HF) outbreak in Uige Province in northern Angola, West Africa. In total, 15 initial...

Enzyme-Linked Immunosorbent Assays for Detection of Antibodies to Ebola and Marburg Viruses Using Recombinant Nucleoproteins

Saijo, Masayuki, Niikura, Masahiro, Morikawa, Shigeru, Ksiazek, Thomas G., Meyer, Richard F., Peters, Clarence J., ...

The full-length nucleoprotein (NP) of Ebola virus (EBO) was expressed as a His-tagged recombinant protein (His-EBO-NP) by a baculovirus system. Carboxy-terminal halves of NPs of EBO and Marburg virus...

Genetic Analysis of the Diversity and Origin of Hantaviruses in Peromyscus leucopus Mice in North America

Morzunov, Sergey P., Rowe, Joan E., Ksiazek, Thomas G., Peters, Clarence J., St. Jeor, Stephen C., Nichol, Stuart T.

Nucleotide sequences were determined for the complete M genome segments of two distinct hantavirus genetic lineages which were detected in hantavirus antibody- and PCR-positive white-footed mice...

Genetic Diversity among Lassa Virus Strains

Bowen, Michael D., Rollin, Pierre E., Ksiazek, Thomas G., Hustad, Heather L., Bausch, Daniel G., Demby, Austin H., ...

The arenavirus Lassa virus causes Lassa fever, a viral hemorrhagic fever that is endemic in the countries of Nigeria, Sierra Leone, Liberia, and Guinea and perhaps elsewhere in West Africa. To...

Satellite imagery characterizes local animal reservoir populations of Sin Nombre virus in the southwestern United States

Glass, Gregory E., Yates, Terry L., Fine, Joshua B., Shields, Timothy M., Kendall, John B., Hope, Andrew G., ...

The relationship between the risk of hantaviral pulmonary syndrome (HPS), as estimated from satellite imagery, and local rodent populations was examined. HPS risk, predicted before rodent sampling,...

Antibody-Dependent Enhancement of Ebola Virus Infection

Takada, Ayato, Feldmann, Heinz, Ksiazek, Thomas G., Kawaoka, Yoshihiro

Most strains of Ebola virus cause a rapidly fatal hemorrhagic disease in humans, yet there are still no biologic explanations that adequately account for the extreme virulence of these emerging...

Viral Discovery and Sequence Recovery Using DNA Microarrays

Wang, David, Urisman, Anatoly, Liu, Yu-Tsueng, Springer, Michael, Ksiazek, Thomas G, Erdman, Dean D, ...

Because of the constant threat posed by emerging infectious diseases and the limitations of existing approaches used to identify new pathogens, there is a great demand for new technological methods...

Rapid Diagnosis of Ebola Hemorrhagic Fever by Reverse Transcription-PCR in an Outbreak Setting and Assessment of Patient Viral Load as a Predictor of Outcome

Towner, Jonathan S., Rollin, Pierre E., Bausch, Daniel G., Sanchez, Anthony, Crary, Sharon M., Vincent, Martin, ...

The largest outbreak on record of Ebola hemorrhagic fever (EHF) occurred in Uganda from August 2000 to January 2001. The outbreak was centered in the Gulu district of northern Uganda, with secondary...

Marburgvirus Genomics and Association with a Large Hemorrhagic Fever Outbreak in Angola

Towner, Jonathan S., Khristova, Marina L., Sealy, Tara K., Vincent, Martin J., Erickson, Bobbie R., Bawiec, Darcy A., ...

In March 2005, the Centers for Disease Control and Prevention (CDC) investigated a large hemorrhagic fever (HF) outbreak in Uige Province in northern Angola, West Africa. In total, 15 initial...

Crimean-Congo Hemorrhagic Fever Virus Genomics and Global Diversity†

Deyde, Varough M., Khristova, Marina L., Rollin, Pierre E., Ksiazek, Thomas G., Nichol, Stuart T.

Crimean-Congo hemorrhagic fever (CCHF) is a severe illness with high case fatality that occurs in Africa, Europe, the Middle East, and Asia. The complete genomes of 13 geographically and temporally...

Hantavirus Pulmonary Syndrome: Pathogenesis of an Emerging Infectious Disease

Zaki, Sherif R., Greer, Patricia W., Coffield, Lisa M., Goldsmith, Cynthia S., Nolte, Kurt B., Foucar, Kathy, ...

A recent outbreak of a severe pulmonary disease in the southwestern United States was etiologically linked to a previously unrecognized bantavirus. The virus has been isolated from its...

Nipah Virus Infection: Pathology and Pathogenesis of an Emerging Paramyxoviral Zoonosis

Wong, Kum Thong, Shieh, Wun-Ju, Kumar, Shalini, Norain, Karim, Abdullah, Wahidah, Guarner, Jeannette, ...

In 1998, an outbreak of acute encephalitis with high mortality rates among pig handlers in Malaysia led to the discovery of a novel paramyxovirus named Nipah virus. A multidisciplinary investigation...

Complete Genome Analysis of 33 Ecologically and Biologically Diverse Rift Valley Fever Virus Strains Reveals Widespread Virus Movement and Low Genetic Diversity due to Recent Common Ancestry▿

Bird, Brian H., Khristova, Marina L., Rollin, Pierre E., Ksiazek, Thomas G., Nichol, Stuart T.

Rift Valley fever (RVF) virus is a mosquito-borne RNA virus responsible for large explosive outbreaks of acute febrile disease in humans and livestock in Africa with significant mortality and...

Andes and Prospect Hill Hantaviruses Differ in Early Induction of Interferon although Both Can Downregulate Interferon Signaling▿

Spiropoulou, Christina F., Albariño, César G., Ksiazek, Thomas G., Rollin, Pierre E.

Hantavirus pulmonary syndrome (HPS) is a severe respiratory disease which is thought to result from a dysregulated immune response to infection with pathogenic hantaviruses, such as Sin Nombre virus...

Marburg Virus Infection Detected in a Common African Bat

Towner, Jonathan S., Pourrut, Xavier, Albariño, César G., Nkogue, Chimène Nze, Bird, Brian H., Grard, Gilda, ...

Marburg and Ebola viruses can cause large hemorrhagic fever (HF) outbreaks with high case fatality (80–90%) in human and great apes. Identification of the natural reservoir of these viruses is one...

Highly Sensitive and Broadly Reactive Quantitative Reverse Transcription-PCR Assay for High-Throughput Detection of Rift Valley Fever Virus▿

Bird, Brian H., Bawiec, Darcy A., Ksiazek, Thomas G., Shoemaker, Trevor R., Nichol, Stuart T.

Rift Valley fever (RVF) virus is a mosquito-borne virus associated with large-scale epizootics/epidemics throughout Africa and the Arabian peninsula. Virus infection can result in economically...

Chapare Virus, a Newly Discovered Arenavirus Isolated from a Fatal Hemorrhagic Fever Case in Bolivia

Delgado, Simon, Erickson, Bobbie R., Agudo, Roberto, Blair, Patrick J., Vallejo, Efrain, Albariño, César G., ...

A small focus of hemorrhagic fever (HF) cases occurred near Cochabamba, Bolivia, in December 2003 and January 2004. Specimens were available from only one fatal case, which had a clinical course that...

Rift Valley Fever Virus Lacking the NSs and NSm Genes Is Highly Attenuated, Confers Protective Immunity from Virulent Virus Challenge, and Allows for Differential Identification of Infected and Vaccinated Animals▿

Bird, Brian H., Albariño, César G., Hartman, Amy L., Erickson, Bobbie Rae, Ksiazek, Thomas G., Nichol, Stuart T.

Rift Valley fever (RVF) virus is a mosquito-borne human and veterinary pathogen associated with large outbreaks of severe disease throughout Africa and more recently the Arabian peninsula. Infection...

Newly Discovered Ebola Virus Associated with Hemorrhagic Fever Outbreak in Uganda

Towner, Jonathan S., Sealy, Tara K., Khristova, Marina L., Albariño, César G., Conlan, Sean, Reeder, Serena A., ...

Over the past 30 years, Zaire and Sudan ebolaviruses have been responsible for large hemorrhagic fever (HF) outbreaks with case fatalities ranging from 53% to 90%, while a third species, Côte...

Framework for Leadership and Training of Biosafety Level 4 Laboratory Workers

Le Duc, James W., Anderson, Kevin, Bloom, Marshall E., Estep, James E., Feldmann, Heinz, Geisbert, Joan B., ...

One-sentence summary for table of contents: Training should include theoretical consideration of biocontainment principles, practical hands-on training, and mentored on-the-job experience.

Multiple Virus Lineages Sharing Recent Common Ancestry Were Associated with a Large Rift Valley Fever Outbreak among Livestock in Kenya during 2006-2007▿ †

Bird, Brian H., Githinji, Jane W. K., Macharia, Joseph M., Kasiiti, Jacqueline L., Muriithi, Rees M., Gacheru, Stephen G., ...

Rift Valley fever (RVF) virus historically has caused widespread and extensive outbreaks of severe human and livestock disease throughout Africa, Madagascar, and the Arabian Peninsula. Following...

Isolation of Genetically Diverse Marburg Viruses from Egyptian Fruit Bats

Towner, Jonathan S., Amman, Brian R., Sealy, Tara K., Carroll, Serena A. Reeder, Comer, James A., Kemp, Alan, ...

In July and September 2007, miners working in Kitaka Cave, Uganda, were diagnosed with Marburg hemorrhagic fever. The likely source of infection in the cave was Egyptian fruit bats (Rousettus...

Nipah Virus Infection in Dogs, Malaysia, 1999

Mills, James N., Alim, Asiah N.M., Bunning, Michel L., Lee, Ong Bee, Wagoner, Kent D., Amman, Brian R., ...

The 1999 outbreak of Nipah virus encephalitis in humans and pigs in Peninsular Malaysia ended with the evacuation of humans and culling of pigs in the epidemic area. Serologic screening showed that,...

Pet Rodents and Fatal Lymphocytic Choriomeningitis in Transplant Patients

Amman, Brian R., Pavlin, Boris I., Albariño, Cesar G., Comer, James A., Erickson, Bobbie R., Oliver, Jennifer B., ...

A unique strain of this virus was traced back to hamsters from an Ohio rodent distribution facility.

An Outbreak of Rift Valley Fever in Northeastern Kenya, 1997-98

Woods, Christopher W., Karpati, Adam M., Grein, Thomas, McCarthy, Noel, Gaturuku, Peter, Muchiri, Eric, ...

In December 1997, 170 hemorrhagic fever-associated deaths were reported in Carissa District, Kenya. Laboratory testing identified evidence of acute Rift Valley fever virus (RVFV). Of the 171 persons...

Antibodies to Nipah-Like Virus in Bats (Pteropus lylei), Cambodia

Olson, James G., Rupprecht, Charles, Rollin, Pierre E., An, Ung Sam, Niezgoda, Michael, Clemins, Travis, ...

Serum specimens from fruit bats were obtained at restaurants in Cambodia. We detected antibodies cross-reactive to Nipah virus by enzyme immunoassay in 11 (11.5%) of 96 Lyle’s flying foxes...

Genetic Analysis of Viruses Associated with Emergence of Rift Valley Fever in Saudi Arabia and Yemen, 2000-01

Shoemaker, Trevor, Boulianne, Carla, Vincent, Martin J., Pezzanite, Linda, Al-Qahtani, Mohammed M., Al-Mazrou, Yagub, ...

The first confirmed Rift Valley fever outbreak outside Africa was reported in September 2000, in the Arabian Peninsula. As of February 2001, a total of 884 hospitalized patients were identified in...

Isolation and Genetic Characterization of Rift Valley fever virus from Aedes vexans arabiensis, Kingdom of Saudi Arabia

Miller, Barry R., Godsey, Marvin S., Crabtree, Mary B., Savage, Harry M., Al-Mazrao, Yagob, Al-Jeffri, Mohammed H., ...

An outbreak of Rift Valley fever in the Kingdom of Saudi Arabia and Yemen in 2000 was the first recognized occurrence of the illness outside of Africa and Madagascar. An assessment of potential...